RLG00000015278

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0012644
Physical Location & Seq
Reverse (-)
63948651 .. 63951275
2625 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015278

Sequence Viewer

Length: 519 bp
ATGCTTTATTGCATGCATGAAGAGGCAATATATATTTCGTACTGCAATTTTCGCAGTCATCAGTATCACCACTGCCGGCCGAAGCAGAAGTATCAAATCGTTGTTTGGGCCTCTTTTGACAATGATTTCAAGGCCGCTGCTCCTTCTCTTTCTCTCGCAACCCTATCCCCGCAGCCCAGCCTTCGCTCCTCTTCTTATCCGGCGGGACCGCCTTCAACGGCATCGTCGAGGAGCTCAAGAATTTCACCACTCGCGTCGCCCACGTCCTTCCAGTTTCCGGCGATGGAGGAAGCACCGCCGAGATTCTCCGCGTCCTCGGTGGTCCGGCCGTCGGAGATACATAGTCCGGCCGACGTATGCATTTGGGAAGCCGTCTTTTTCAAAGAAAAAAAACAGATCTTTTCTTTCCTCCAGATTTTGCAGATGATTTCCCAGTTGCCATACAGCTTAAAGAGGAAAGGAATCAAGATAGCTAGGAAGAGGGATGTCATAAGGAACACTACAGGAAACCTTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

173

Amino Acids

19.52

Weight (kDa)

9.79

Isoelectric Point (pI)

74.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 254, 311
AciI CCGC 6 cut(s) 135, 170, 203, 209, 296, 309
AcoI YGGCCR 3 cut(s) 77, 326, 348
AcsI RAATTY 1 cut(s) 240
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 2 cut(s) 277, 331
AgsI TTSAA 3 cut(s) 130, 216, 382
AjiI CACGTC 1 cut(s) 264
AluBI AGCT 3 cut(s) 234, 447, 473
AluI AGCT 3 cut(s) 234, 447, 473
Alw21I GWGCWC 1 cut(s) 236
AoxI GGCC 5 cut(s) 77, 108, 132, 326, 348
ApeKI GCWGC 2 cut(s) 137, 172
ApoI RAATTY 1 cut(s) 240
AspS9I GGNCC 3 cut(s) 108, 206, 322
AsuHPI GGTGA 2 cut(s) 59, 237
AvaII GGWCC 2 cut(s) 206, 322
BanII GRGCYC 1 cut(s) 236
Bbv12I GWGCWC 1 cut(s) 236
BbvI GCAGC 2 cut(s) 124, 184
BccI CCATC 1 cut(s) 277
BceAI ACGGC 3 cut(s) 234, 313, 356
BfaI CTAG 1 cut(s) 474
BfmI CTRYAG 1 cut(s) 501
BglII AGATCT 1 cut(s) 396
BisI GCNGC 3 cut(s) 135, 138, 173
BlsI GCNGC 3 cut(s) 136, 139, 174
Bme18I GGWCC 2 cut(s) 206, 322
BmgBI CACGTC 1 cut(s) 264
BmgT120I GGNCC 3 cut(s) 108, 206, 322
BmiI GGNNCC 1 cut(s) 207
BmrI ACTGGG 1 cut(s) 427
BmsI GCATC 1 cut(s) 230
BmuI ACTGGG 1 cut(s) 427
BpmI CTGGAG 1 cut(s) 395
BpuEI CTTGAG 1 cut(s) 220
BsaJI CCNNGG 1 cut(s) 315
Bsc4I CCNNNNNNNGG 2 cut(s) 277, 331
Bse118I RCCGGY 1 cut(s) 75
Bse1I ACTGG 2 cut(s) 271, 433
BseDI CCNNGG 1 cut(s) 315
BseGI GGATG 1 cut(s) 490
BseLI CCNNNNNNNGG 2 cut(s) 277, 331
BseNI ACTGG 2 cut(s) 271, 433
BseRI GAGGAG 2 cut(s) 178, 244
BseX3I CGGCCG 3 cut(s) 77, 326, 348
BseXI GCAGC 2 cut(s) 124, 184
BseYI CCCAGC 1 cut(s) 176
Bsh1236I CGCG 2 cut(s) 254, 311
Bsh1285I CGRYCG 3 cut(s) 80, 329, 351
BshFI GGCC 5 cut(s) 79, 110, 134, 328, 350
BsiEI CGRYCG 3 cut(s) 80, 329, 351
BsiHKAI GWGCWC 1 cut(s) 236
BsiSI CCGG 5 cut(s) 76, 200, 278, 325, 347
BslFI GGGAC 1 cut(s) 219
BslI CCNNNNNNNGG 2 cut(s) 277, 331
BsmFI GGGAC 1 cut(s) 219
BsnI GGCC 5 cut(s) 79, 110, 134, 328, 350
Bsp1286I GDGCHC 1 cut(s) 236
Bsp143I GATC 1 cut(s) 396
BspACI CCGC 6 cut(s) 135, 170, 203, 209, 296, 309
BspANI GGCC 5 cut(s) 79, 110, 134, 328, 350
BspFNI CGCG 2 cut(s) 254, 311
BspLI GGNNCC 1 cut(s) 207
BsrFI RCCGGY 1 cut(s) 75
BsrI ACTGG 2 cut(s) 271, 433
BssAI RCCGGY 1 cut(s) 75
BssECI CCNNGG 1 cut(s) 315
BssMI GATC 1 cut(s) 396
Bst6I CTCTTC 3 cut(s) 15, 196, 473
BstC8I GCNNGC 2 cut(s) 14, 77
BstDEI CTNAG 1 cut(s) 512
BstF5I GGATG 1 cut(s) 490
BstFNI CGCG 2 cut(s) 254, 311
BstKTI GATC 1 cut(s) 399
BstMBI GATC 1 cut(s) 396
BstMCI CGRYCG 3 cut(s) 80, 329, 351
BstMWI GCNNNNNNNGC 1 cut(s) 51
BstNSI RCATGY 1 cut(s) 16
BstSFI CTRYAG 1 cut(s) 501
BstUI CGCG 2 cut(s) 254, 311
BstV1I GCAGC 2 cut(s) 124, 184
BstX2I RGATCY 1 cut(s) 396
BstYI RGATCY 1 cut(s) 396
BstZI CGGCCG 3 cut(s) 77, 326, 348
BsuRI GGCC 5 cut(s) 79, 110, 134, 328, 350
BtgZI GCGATG 1 cut(s) 296
BtrI CACGTC 1 cut(s) 264
BtsCI GGATG 1 cut(s) 490
BtsI GCAGTG 1 cut(s) 70
BtsIMutI CAGTG 1 cut(s) 70
Cac8I GCNNGC 2 cut(s) 14, 77
Cfr10I RCCGGY 1 cut(s) 75
Cfr13I GGNCC 3 cut(s) 108, 206, 322
CseI GACGC 2 cut(s) 243, 300
Csp6I GTAC 1 cut(s) 40
CviAII CATG 2 cut(s) 13, 17
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 1 cut(s) 512
DpnI GATC 1 cut(s) 398
DpnII GATC 1 cut(s) 396
EaeI YGGCCR 3 cut(s) 77, 326, 348
EagI CGGCCG 3 cut(s) 77, 326, 348
Eam1104I CTCTTC 3 cut(s) 15, 196, 473
EarI CTCTTC 3 cut(s) 15, 196, 473
Ecl136II GAGCTC 1 cut(s) 234
EclXI CGGCCG 3 cut(s) 77, 326, 348
Eco24I GRGCYC 1 cut(s) 236
Eco47I GGWCC 2 cut(s) 206, 322
Eco52I CGGCCG 3 cut(s) 77, 326, 348
Eco53kI GAGCTC 1 cut(s) 234
EcoICRI GAGCTC 1 cut(s) 234
EcoT22I ATGCAT 2 cut(s) 18, 362
EcoT38I GRGCYC 1 cut(s) 236
FaeI CATG 2 cut(s) 16, 20
FaiI YATR 8 cut(s) 14, 18, 31, 33, 342, 358, 442, 491
FaqI GGGAC 1 cut(s) 219
FatI CATG 2 cut(s) 12, 16
FauI CCCGC 2 cut(s) 177, 196
Fnu4HI GCNGC 3 cut(s) 135, 138, 173
FokI GGATG 1 cut(s) 497
FriOI GRGCYC 1 cut(s) 236
Fsp4HI GCNGC 3 cut(s) 135, 138, 173
FspBI CTAG 1 cut(s) 474
GluI GCNGC 3 cut(s) 135, 138, 173
GsaI CCCAGC 1 cut(s) 180
GsuI CTGGAG 1 cut(s) 395
HaeIII GGCC 5 cut(s) 79, 110, 134, 328, 350
HapII CCGG 5 cut(s) 76, 200, 278, 325, 347
HgaI GACGC 2 cut(s) 243, 300
Hin1II CATG 2 cut(s) 16, 20
HinfI GANTC 2 cut(s) 303, 462
HpaII CCGG 5 cut(s) 76, 200, 278, 325, 347
HphI GGTGA 2 cut(s) 59, 237
Hpy188I TCNGA 1 cut(s) 334
Hpy188III TCNNGA 3 cut(s) 237, 412, 466
Hpy99I CGWCG 4 cut(s) 229, 259, 334, 356
HpyAV CCTTC 4 cut(s) 153, 191, 222, 277
HpyCH4IV ACGT 2 cut(s) 263, 354
HpyCH4V TGCA 5 cut(s) 12, 16, 45, 360, 421
HpyF10VI GCNNNNNNNGC 1 cut(s) 51
HpyF3I CTNAG 1 cut(s) 512
HpySE526I ACGT 2 cut(s) 263, 354
Hsp92II CATG 2 cut(s) 16, 20
KroI GCCGGC 1 cut(s) 75
KroNI GCCGGC 1 cut(s) 77
Kzo9I GATC 1 cut(s) 396
LmnI GCTCC 3 cut(s) 145, 191, 231
Lsp1109I GCAGC 2 cut(s) 124, 184
LweI GCATC 1 cut(s) 230
MaeI CTAG 1 cut(s) 474
MaeII ACGT 2 cut(s) 263, 354
MalI GATC 1 cut(s) 398
MboI GATC 1 cut(s) 396
MboII GAAGA 3 cut(s) 32, 183, 490
MflI RGATCY 1 cut(s) 396
MhlI GDGCHC 1 cut(s) 236
MluCI AATT 2 cut(s) 46, 240
MmeI TCCRAC 1 cut(s) 312
MnlI CCTC 9 cut(s) 16, 121, 199, 222, 280, 325, 419, 447, 474
Mph1103I ATGCAT 2 cut(s) 18, 362
MroNI GCCGGC 1 cut(s) 75
MseI TTAA 1 cut(s) 449
MspA1I CMGCKG 1 cut(s) 137
MspI CCGG 5 cut(s) 76, 200, 278, 325, 347
MvnI CGCG 2 cut(s) 254, 311
MwoI GCNNNNNNNGC 1 cut(s) 51
NaeI GCCGGC 1 cut(s) 77
NdeII GATC 1 cut(s) 396
NgoMIV GCCGGC 1 cut(s) 75
NlaIII CATG 2 cut(s) 16, 20
NlaIV GGNNCC 1 cut(s) 207
NmeAIII GCCGAG 1 cut(s) 324
NsiI ATGCAT 2 cut(s) 18, 362
NspI RCATGY 1 cut(s) 16
PaeI GCATGC 1 cut(s) 16
PcsI WCGNNNNNNNCGW 1 cut(s) 224
PdiI GCCGGC 1 cut(s) 77
PfeI GAWTC 2 cut(s) 303, 462
PkrI GCNGC 3 cut(s) 136, 139, 174
Psp124BI GAGCTC 1 cut(s) 236
PspFI CCCAGC 1 cut(s) 176
PspN4I GGNNCC 1 cut(s) 207
PspPI GGNCC 3 cut(s) 108, 206, 322
PsuI RGATCY 1 cut(s) 396
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
SacI GAGCTC 1 cut(s) 236
SaqAI TTAA 1 cut(s) 449
SatI GCNGC 3 cut(s) 135, 138, 173
Sau3AI GATC 1 cut(s) 396
Sau96I GGNCC 3 cut(s) 108, 206, 322
SduI GDGCHC 1 cut(s) 236
SetI ASST 6 cut(s) 236, 266, 357, 449, 475, 513
SfaNI GCATC 1 cut(s) 230
SfcI CTRYAG 1 cut(s) 501
SinI GGWCC 2 cut(s) 206, 322
SmlI CTYRAG 1 cut(s) 235
SmoI CTYRAG 1 cut(s) 235
SphI GCATGC 1 cut(s) 16
Sse9I AATT 2 cut(s) 46, 240
SsiI CCGC 6 cut(s) 135, 170, 203, 209, 296, 309
SspMI CTAG 1 cut(s) 474
SstI GAGCTC 1 cut(s) 236
TaiI ACGT 2 cut(s) 266, 357
TaqI TCGA 1 cut(s) 227
TasI AATT 2 cut(s) 46, 240
TauI GCSGC 1 cut(s) 137
TfiI GAWTC 2 cut(s) 303, 462
Tru1I TTAA 1 cut(s) 449
Tru9I TTAA 1 cut(s) 449
TscAI CASTG 1 cut(s) 77
TseI GCWGC 2 cut(s) 137, 172
TspDTI ATGAA 1 cut(s) 33
TspRI CASTG 1 cut(s) 77
VpaK11BI GGWCC 2 cut(s) 206, 322
XapI RAATTY 1 cut(s) 240
XceI RCATGY 1 cut(s) 16
XspI CTAG 1 cut(s) 474
Zsp2I ATGCAT 2 cut(s) 18, 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.