Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004036

Physical Location & Seq

Genomic Coordinates Forward (+)

44649516 .. 44654765

Gene Structure 5250 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1318200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1725 bp

ATGACTGCGGAGGTTCTCTCGCAGCCAAACGGCGTCGTCGCGAATGGCGACCTGAACGCAATCCCTACCAACAACGCCACCGCCGCCAAGAAGTCGAGGGAGAGCGAGCGACGTCGTCGCAGGAGAAAGCAGAAGAAGAACAACAAGGCCTCTCAGGCACCGGAGTCCAGCGCCGGCGAGACCGACGATGACGTCAAGGAGAACAACGATCCTCAACAGATTATTGAGCAAGTTCAAATTGAATATGTTCCAGAGAAACCTGAGCTAAGTGATGGCATGGATGAGGAATTCAGAAAAATTTTCGAGAAATTCTGCTTTCAGGACTCCGTGGGTGTTGAGGAGGATAAGAAGGATGAGTCTGAAGGAGCCCCGAAAAAGAAGGCTGACTCAGATTCTGAAGAGGAAGAGCAGGATAATGAACAAAAAGAGAAAGGCATATCAAACAAGAAGAAAAAGCTTCAACGGAGAATGAAGATTGCCGAATTGAAACAGATCTGCCAAAGGCCTGATGTTGTTGAGGTGTGGGATGCAACTGCAGCTGATCCTAAGCTGCTGGTGTTTCTAAAATCATATCGCAATACTGTACCTGTGCCAAGACATTGGTGTCAAAAAAGGAAATTTCTGCAGGGTAAACGTGGTATTGAGAAACAACCGTTTCAGCTTCCCGACTTCATTGCTGCTACTGGAATTGAGAAAATTAGACAGGCATACATTGAAAAAGAAGATAGTAAGAAGTTGAAGCAAAAACAACGAGAGCGTATGCAACCAAAGATGGGGAAAATGGATATTGATTATCAGGTTCTCCATGATGCATTTTTCAAGTACCAGACTAAGCCAAAGTTGACGACTCTTGGTGATCTGTACCATGAAGGGAAGGAGTTTGAGGTGAAATTGAGGGAGATGAAACCAGGCATGCTTTCACATGAACTAAAAGAAGCTCTGGGTATGCCAGATGGTGCTCCTCCCCCGTGGCTCATCAATATGCAGAGATATGGTCCTCCACCATCATATCCACATCTGAAAATTCCCGGGCTGAATGCTCCAATCCCTCCGGGTGCTAGCTTTGGTTACCATCCTGGTGGCTGGGGAAAGCCACCTGTTGATGAATATGGCCAACCGTTGTATGGGGATGTATTTGGCGTTCAGCAACAAGATCAGCCCAATTATGAGGAGCCTGTTGACAAGACAAAGCATTGGGGTGATTTGGAGGAAGAGGAGGAGGAGGAAGAAGAAGAGGAGGAGGAAGAGGAAGAACAGCCTGAGGAAGAAGATTTAGATGATGGCATTCAATCTGTAGACAGCCTCTCAAGTACTCCCACCGGCGTTGAGACACCTGCTCAAATTGAACTGCGCAAGGATCAGAAAAAAGAATCTGAAAAGCCTCTATACCAAGTACTTGAACAAAAAGAGGAAAGAATTGCTCCGGGGACACTTCTTGCAACGGGACACACGTATAATTTGAGTGGCACCCAAGACAAAGCAGGGCCCAAAAAGGTTGAGCTGCTTAGAGGTCAGAAAGCAGATAAAGTGGAGGTCACTATACAACCCGAAGAATTGGATGATATGGAGACCGTTTTGCCTGCAAAGTACGAAGAAGCAAGGGAGGAGGAGAAGCTGCGAAGTCAGAAGGAAGATTTCAGTGACATGGTCGCAGAGAACGAGAAGAAGAGGAAACGTAAGCAAGACAAGGAAGGGAAAAACAAGAAGAGGGACTTCAAGTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000375 GO:0000377 GO:0000398 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0005686 GO:0005689 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016604 GO:0016607 GO:0030532 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0070013 GO:0071011 GO:0071013 GO:0071704 GO:0090304 GO:0097525 GO:0120114 GO:1901360 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DUF382 PSP

Protein Analysis

575

Amino Acids

65.99

Weight (kDa)

5.18

Isoelectric Point (pI)

61.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF382	PF04037	167 - 293	9.2e-60	Domain of unknown function (DUF382)
PSP	PF04046	302 - 347	9.9e-23	PSP

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004036)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11520 AT4G21660 AT4G21660 AT4G21660
fragaria_vesca	FvH4_3g04740 FvH4_6g45300
malus_domestica	MD05G1318200.v1.1 MD10G1297600.v1.1
prunus_persica	Prupe.4G042600_v2.0.a1 Prupe.4G042600_v2.0.a1
pyrus_communis	pycom05g29460 pycom10g25110
rosa_chinensis	RchiOBHm_Chr2g0163041 RchiOBHm_Chr5g0007761
rosa_laevigata	RLG00000021367 RLG00000031486
rosa_multiflora	Rmu_co8286435.1_g000001 Rmu_co8351911.1_g000001 Rmu_sc0005098.1_g000019 Rmu_sc0007039.1_g000007
rosa_roxburghii	Rroxscaffold_1G00068650 Rroxscaffold_2G00087600
rosa_rugosa	Rorug02G0500000.1 Rorug04G0430100
rosa_samantha	Rh2AG566300 Rh2BG578600 Rh2CG548500 Rh5AG058500 Rh5BG055300 Rh5CG065100 Rh5DG054300
rosa_wichuraiana	Rw2G046870 Rw5G006120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1342
AasI	GACNNNNNNGTC	2 cut(s)	191, 603
AatII	GACGTC	2 cut(s)	115, 195
Acc16I	TGCGCA	1 cut(s)	1352
Acc36I	ACCTGC	1 cut(s)	1342
AccB1I	GGYRCC	2 cut(s)	157, 1466
AccI	GTMKAC	1 cut(s)	1296
AccII	CGCG	1 cut(s)	41
AciI	CCGC	3 cut(s)	8, 81, 84
AclWI	GGATC	3 cut(s)	203, 536, 1365
AcoI	YGGCCR	1 cut(s)	1111
AcsI	RAATTY	5 cut(s)	287, 297, 308, 617, 1023
AcuI	CTGAAG	2 cut(s)	381, 417
AcyI	GRCGYC	3 cut(s)	33, 112, 192
AfaI	GTAC	6 cut(s)	585, 824, 863, 1312, 1395, 1589
AfiI	CCNNNNNNNGG	2 cut(s)	773, 1124
AflIII	ACRYGT	1 cut(s)	1449
AjnI	CCWGG	2 cut(s)	907, 1075
AluBI	AGCT	9 cut(s)	265, 457, 539, 550, 661, 938, 1062, 1501, 1615
AluI	AGCT	9 cut(s)	265, 457, 539, 550, 661, 938, 1062, 1501, 1615
Alw21I	GWGCWC	1 cut(s)	961
Alw26I	GTCTC	3 cut(s)	173, 1322, 1562
AlwI	GGATC	3 cut(s)	203, 536, 1365
AlwNI	CAGNNNCTG	1 cut(s)	395
Ama87I	CYCGRG	1 cut(s)	1028
AoxI	GGCC	4 cut(s)	147, 503, 1111, 1484
ApaI	GGGCCC	1 cut(s)	1488
ApeKI	GCWGC	6 cut(s)	22, 536, 550, 677, 1501, 1615
ApoI	RAATTY	5 cut(s)	287, 297, 308, 617, 1023
Asp700I	GAANNNNTTC	1 cut(s)	246
AspLEI	GCGC	2 cut(s)	173, 1353
AspS9I	GGNCC	3 cut(s)	995, 1484, 1485
AsuC2I	CCSGG	4 cut(s)	1029, 1030, 1053, 1425
AsuHPI	GGTGA	3 cut(s)	866, 898, 1211
AsuNHI	GCTAGC	1 cut(s)	1058
AvaI	CYCGRG	1 cut(s)	1028
AvaII	GGWCC	1 cut(s)	995
AxyI	CCTNAGG	1 cut(s)	1260
BaeGI	GKGCMC	1 cut(s)	1488
BalI	TGGCCA	1 cut(s)	1113
BanI	GGYRCC	2 cut(s)	157, 1466
BanII	GRGCYC	2 cut(s)	370, 1488
Bbv12I	GWGCWC	1 cut(s)	961
BbvI	GCAGC	6 cut(s)	34, 537, 548, 664, 1488, 1602
BccI	CCATC	6 cut(s)	266, 766, 947, 1012, 1080, 1274
BceAI	ACGGC	1 cut(s)	46
BcgI	CGANNNNNNTGC	2 cut(s)	656, 690
BciT130I	CCWGG	2 cut(s)	909, 1077
BcnI	CCSGG	4 cut(s)	1029, 1030, 1053, 1425
BcoDI	GTCTC	3 cut(s)	173, 1322, 1562
BfaI	CTAG	1 cut(s)	1059
BfmI	CTRYAG	3 cut(s)	534, 623, 1293
BfoI	RGCGCY	1 cut(s)	174
BfuAI	ACCTGC	1 cut(s)	1342
BglI	GCCNNNNNGGC	1 cut(s)	155
BglII	AGATCT	1 cut(s)	492
BisI	GCNGC	7 cut(s)	23, 84, 537, 551, 678, 1502, 1616
BlsI	GCNGC	7 cut(s)	24, 85, 538, 552, 679, 1503, 1617
BmcAI	AGTACT	2 cut(s)	1312, 1395
Bme1390I	CCNGG	6 cut(s)	909, 1029, 1030, 1053, 1077, 1425
Bme18I	GGWCC	1 cut(s)	995
BmeT110I	CYCGRG	1 cut(s)	1028
BmgT120I	GGNCC	3 cut(s)	995, 1484, 1485
BmiI	GGNNCC	5 cut(s)	159, 367, 1173, 1468, 1486
BmrFI	CCNGG	6 cut(s)	909, 1029, 1030, 1053, 1077, 1425
BmsI	GCATC	2 cut(s)	517, 799
BmtI	GCTAGC	1 cut(s)	1062
BplI	GAGNNNNNCTC	1 cut(s)	34
Bpu10I	CCTNAGC	2 cut(s)	261, 546
BpuEI	CTTGAG	1 cut(s)	1291
BpuMI	CCSGG	4 cut(s)	1029, 1030, 1053, 1425
BsaAI	YACGTR	1 cut(s)	1452
BsaHI	GRCGYC	3 cut(s)	33, 112, 192
BsaI	GGTCTC	2 cut(s)	173, 1562
BsaJI	CCNNGG	4 cut(s)	327, 968, 1028, 1424
BsaWI	WCCGGW	1 cut(s)	160
Bsc4I	CCNNNNNNNGG	2 cut(s)	773, 1124
Bse118I	RCCGGY	2 cut(s)	173, 1319
Bse1I	ACTGG	1 cut(s)	688
Bse21I	CCTNAGG	1 cut(s)	1260
Bse3DI	GCAATG	1 cut(s)	672
BseBI	CCWGG	2 cut(s)	909, 1077
BseDI	CCNNGG	4 cut(s)	327, 968, 1028, 1424
BseGI	GGATG	6 cut(s)	286, 358, 532, 1072, 1135, 1564
BseLI	CCNNNNNNNGG	2 cut(s)	773, 1124
BseMI	GCAATG	1 cut(s)	672
BseMII	CTCAG	4 cut(s)	167, 252, 402, 1251
BseNI	ACTGG	1 cut(s)	688
BseSI	GKGCMC	1 cut(s)	1488
BseXI	GCAGC	6 cut(s)	34, 537, 548, 664, 1488, 1602
BseYI	CCCAGC	1 cut(s)	1083
Bsh1236I	CGCG	1 cut(s)	41
BshFI	GGCC	4 cut(s)	149, 505, 1113, 1486
BshNI	GGYRCC	2 cut(s)	157, 1466
BsiHKAI	GWGCWC	1 cut(s)	961
BsiHKCI	CYCGRG	1 cut(s)	1028
BsiSI	CCGG	6 cut(s)	161, 174, 1029, 1052, 1320, 1424
BslFI	GGGAC	2 cut(s)	1441, 1458
BslI	CCNNNNNNNGG	2 cut(s)	773, 1124
BsmAI	GTCTC	3 cut(s)	173, 1322, 1562
BsmFI	GGGAC	2 cut(s)	1441, 1458
BsmI	GAATGC	2 cut(s)	1042, 1284
BsnI	GGCC	4 cut(s)	149, 505, 1113, 1486
Bso31I	GGTCTC	2 cut(s)	173, 1562
BsoBI	CYCGRG	1 cut(s)	1028
Bsp120I	GGGCCC	1 cut(s)	1484
Bsp1286I	GDGCHC	3 cut(s)	370, 961, 1488
Bsp143I	GATC	6 cut(s)	208, 492, 541, 856, 1153, 1357
Bsp68I	TCGCGA	1 cut(s)	41
BspACI	CCGC	3 cut(s)	8, 81, 84
BspANI	GGCC	4 cut(s)	149, 505, 1113, 1486
BspCNI	CTCAG	4 cut(s)	166, 253, 401, 1252
BspFNI	CGCG	1 cut(s)	41
BspLI	GGNNCC	5 cut(s)	159, 367, 1173, 1468, 1486
BspMAI	CTGCAG	2 cut(s)	538, 627
BspMI	ACCTGC	1 cut(s)	1342
BspOI	GCTAGC	1 cut(s)	1062
BspPI	GGATC	3 cut(s)	203, 536, 1365
BspQI	GCTCTTC	1 cut(s)	399
BspT107I	GGYRCC	2 cut(s)	157, 1466
BspTNI	GGTCTC	2 cut(s)	173, 1562
BsrDI	GCAATG	1 cut(s)	672
BsrFI	RCCGGY	2 cut(s)	173, 1319
BsrI	ACTGG	1 cut(s)	688
BssAI	RCCGGY	2 cut(s)	173, 1319
BssECI	CCNNGG	4 cut(s)	327, 968, 1028, 1424
BssMI	GATC	6 cut(s)	208, 492, 541, 856, 1153, 1357
BssNI	GRCGYC	3 cut(s)	33, 112, 192
Bst2UI	CCWGG	2 cut(s)	909, 1077
Bst4CI	ACNGT	4 cut(s)	583, 654, 1119, 1573
Bst6I	CTCTTC	7 cut(s)	393, 399, 1206, 1227, 1239, 1661, 1700
BstACI	GRCGYC	3 cut(s)	33, 112, 192
BstBAI	YACGTR	1 cut(s)	1452
BstC8I	GCNNGC	5 cut(s)	107, 175, 914, 1060, 1581
BstDEI	CTNAG	8 cut(s)	153, 261, 266, 388, 546, 831, 1260, 1505
BstDSI	CCRYGG	2 cut(s)	327, 968
BstEII	GGTNACC	1 cut(s)	1067
BstF5I	GGATG	6 cut(s)	286, 358, 532, 1072, 1135, 1564
BstFNI	CGCG	1 cut(s)	41
BstH2I	RGCGCY	1 cut(s)	174
BstHHI	GCGC	2 cut(s)	173, 1353
BstKTI	GATC	6 cut(s)	211, 495, 544, 859, 1156, 1360
BstMAI	GTCTC	3 cut(s)	173, 1322, 1562
BstMBI	GATC	6 cut(s)	208, 492, 541, 856, 1153, 1357
BstMWI	GCNNNNNNNGC	3 cut(s)	83, 155, 536
BstNI	CCWGG	2 cut(s)	909, 1077
BstNSI	RCATGY	1 cut(s)	916
BstPI	GGTNACC	1 cut(s)	1067
BstSCI	CCNGG	6 cut(s)	907, 1027, 1028, 1051, 1075, 1423
BstSFI	CTRYAG	3 cut(s)	534, 623, 1293
BstSLI	GKGCMC	1 cut(s)	1488
BstUI	CGCG	1 cut(s)	41
BstV1I	GCAGC	6 cut(s)	34, 537, 548, 664, 1488, 1602
BstX2I	RGATCY	1 cut(s)	492
BstXI	CCANNNNNNTGG	2 cut(s)	600, 1079
BstYI	RGATCY	1 cut(s)	492
Bsu36I	CCTNAGG	1 cut(s)	1260
BsuRI	GGCC	4 cut(s)	149, 505, 1113, 1486
BtgI	CCRYGG	2 cut(s)	327, 968
BtsCI	GGATG	6 cut(s)	286, 358, 532, 1072, 1135, 1564
BtsIMutI	CAGTG	1 cut(s)	1645
BtuMI	TCGCGA	1 cut(s)	41
BveI	ACCTGC	1 cut(s)	1342
Cac8I	GCNNGC	5 cut(s)	107, 175, 914, 1060, 1581
CaiI	CAGNNNCTG	1 cut(s)	395
CfoI	GCGC	2 cut(s)	173, 1353
Cfr10I	RCCGGY	2 cut(s)	173, 1319
Cfr13I	GGNCC	3 cut(s)	995, 1484, 1485
Cfr9I	CCCGGG	1 cut(s)	1028
CseI	GACGC	1 cut(s)	22
Csp6I	GTAC	6 cut(s)	584, 823, 862, 1311, 1394, 1588
CspCI	CAANNNNNGTGG	2 cut(s)	1306, 1341
CviAII	CATG	6 cut(s)	277, 806, 866, 913, 923, 1645
CviQI	GTAC	6 cut(s)	584, 823, 862, 1311, 1394, 1588
DdeI	CTNAG	8 cut(s)	153, 261, 266, 388, 546, 831, 1260, 1505
DpnI	GATC	6 cut(s)	210, 494, 543, 858, 1155, 1359
DpnII	GATC	6 cut(s)	208, 492, 541, 856, 1153, 1357
DrdI	GACNNNNNNGTC	2 cut(s)	191, 603
DseDI	GACNNNNNNGTC	2 cut(s)	191, 603
EaeI	YGGCCR	1 cut(s)	1111
Eam1104I	CTCTTC	7 cut(s)	393, 399, 1206, 1227, 1239, 1661, 1700
EarI	CTCTTC	7 cut(s)	393, 399, 1206, 1227, 1239, 1661, 1700
Eco147I	AGGCCT	2 cut(s)	149, 505
Eco24I	GRGCYC	2 cut(s)	370, 1488
Eco31I	GGTCTC	2 cut(s)	173, 1562
Eco47I	GGWCC	1 cut(s)	995
Eco57I	CTGAAG	2 cut(s)	381, 417
Eco81I	CCTNAGG	1 cut(s)	1260
Eco88I	CYCGRG	1 cut(s)	1028
Eco91I	GGTNACC	1 cut(s)	1067
EcoO109I	RGGNCCY	1 cut(s)	1484
EcoO65I	GGTNACC	1 cut(s)	1067
EcoRI	GAATTC	1 cut(s)	287
EcoRII	CCWGG	2 cut(s)	907, 1075
EcoT22I	ATGCAT	1 cut(s)	814
EcoT38I	GRGCYC	2 cut(s)	370, 1488
FaeI	CATG	6 cut(s)	280, 809, 869, 916, 926, 1648
FalI	AAGNNNNNCTT	1 cut(s)	1697
FaqI	GGGAC	2 cut(s)	1441, 1458
FatI	CATG	6 cut(s)	276, 805, 865, 912, 922, 1644
FblI	GTMKAC	1 cut(s)	1296
Fnu4HI	GCNGC	7 cut(s)	23, 84, 537, 551, 678, 1502, 1616
FokI	GGATG	6 cut(s)	293, 365, 539, 1059, 1142, 1571
FriOI	GRGCYC	2 cut(s)	370, 1488
Fsp4HI	GCNGC	7 cut(s)	23, 84, 537, 551, 678, 1502, 1616
FspBI	CTAG	1 cut(s)	1059
FspI	TGCGCA	1 cut(s)	1352
GlaI	GCGC	2 cut(s)	172, 1352
GluI	GCNGC	7 cut(s)	23, 84, 537, 551, 678, 1502, 1616
GsaI	CCCAGC	1 cut(s)	1087
HaeII	RGCGCY	1 cut(s)	174
HaeIII	GGCC	4 cut(s)	149, 505, 1113, 1486
HapII	CCGG	6 cut(s)	161, 174, 1029, 1052, 1320, 1424
HgaI	GACGC	1 cut(s)	22
HhaI	GCGC	2 cut(s)	173, 1353
Hin1I	GRCGYC	3 cut(s)	33, 112, 192
Hin1II	CATG	6 cut(s)	280, 809, 869, 916, 926, 1648
Hin6I	GCGC	2 cut(s)	171, 1351
HinP1I	GCGC	2 cut(s)	171, 1351
HincII	GTYRAC	2 cut(s)	843, 1180
HindII	GTYRAC	2 cut(s)	843, 1180
HindIII	AAGCTT	1 cut(s)	455
HinfI	GANTC	7 cut(s)	164, 323, 356, 386, 392, 847, 1370
HpaII	CCGG	6 cut(s)	161, 174, 1029, 1052, 1320, 1424
HphI	GGTGA	3 cut(s)	866, 898, 1211
Hpy166II	GTNNAC	4 cut(s)	632, 843, 1180, 1297
Hpy188I	TCNGA	9 cut(s)	293, 361, 391, 397, 1020, 1362, 1375, 1515, 1626
Hpy188III	TCNNGA	5 cut(s)	40, 251, 304, 320, 665
Hpy8I	GTNNAC	4 cut(s)	632, 843, 1180, 1297
Hpy99I	CGWCG	6 cut(s)	38, 41, 114, 117, 120, 188
HpyAV	CCTTC	7 cut(s)	343, 356, 373, 863, 868, 1621, 1685
HpyCH4III	ACNGT	4 cut(s)	583, 654, 1119, 1573
HpyCH4IV	ACGT	5 cut(s)	112, 192, 634, 1451, 1675
HpyCH4V	TGCA	8 cut(s)	530, 536, 625, 763, 812, 985, 1439, 1583
HpyF10VI	GCNNNNNNNGC	3 cut(s)	83, 155, 536
HpyF3I	CTNAG	8 cut(s)	153, 261, 266, 388, 546, 831, 1260, 1505
HpySE526I	ACGT	5 cut(s)	112, 192, 634, 1451, 1675
Hsp92I	GRCGYC	3 cut(s)	33, 112, 192
Hsp92II	CATG	6 cut(s)	280, 809, 869, 916, 926, 1648
HspAI	GCGC	2 cut(s)	171, 1351
KroI	GCCGGC	1 cut(s)	173
KroNI	GCCGGC	1 cut(s)	175
Kzo9I	GATC	6 cut(s)	208, 492, 541, 856, 1153, 1357
LguI	GCTCTTC	1 cut(s)	399
LmnI	GCTCC	5 cut(s)	365, 964, 1045, 1171, 1426
Lsp1109I	GCAGC	6 cut(s)	34, 537, 548, 664, 1488, 1602
LweI	GCATC	2 cut(s)	517, 799
MaeI	CTAG	1 cut(s)	1059
MaeII	ACGT	5 cut(s)	112, 192, 634, 1451, 1675
MaeIII	GTNAC	3 cut(s)	1067, 1534, 1640
MalI	GATC	6 cut(s)	210, 494, 543, 858, 1155, 1359
MboI	GATC	6 cut(s)	208, 492, 541, 856, 1153, 1357
MflI	RGATCY	1 cut(s)	492
MhlI	GDGCHC	3 cut(s)	370, 961, 1488
MlsI	TGGCCA	1 cut(s)	1113
MluNI	TGGCCA	1 cut(s)	1113
MlyI	GAGTC	5 cut(s)	173, 317, 365, 380, 841
Mox20I	TGGCCA	1 cut(s)	1113
Mph1103I	ATGCAT	1 cut(s)	814
MreI	CGCCGGCG	1 cut(s)	173
MroNI	GCCGGC	1 cut(s)	173
MroXI	GAANNNNTTC	1 cut(s)	246
MscI	TGGCCA	1 cut(s)	1113
MslI	CAYNNNNRTG	3 cut(s)	980, 1077, 1197
Msp20I	TGGCCA	1 cut(s)	1113
MspA1I	CMGCKG	1 cut(s)	539
MspI	CCGG	6 cut(s)	161, 174, 1029, 1052, 1320, 1424
MspR9I	CCNGG	6 cut(s)	909, 1029, 1030, 1053, 1077, 1425
Mva1269I	GAATGC	2 cut(s)	1042, 1284
MvaI	CCWGG	2 cut(s)	909, 1077
MvnI	CGCG	1 cut(s)	41
MwoI	GCNNNNNNNGC	3 cut(s)	83, 155, 536
NaeI	GCCGGC	1 cut(s)	175
NciI	CCSGG	4 cut(s)	1029, 1030, 1053, 1425
NdeII	GATC	6 cut(s)	208, 492, 541, 856, 1153, 1357
NgoMIV	GCCGGC	1 cut(s)	173
NheI	GCTAGC	1 cut(s)	1058
NlaIII	CATG	6 cut(s)	280, 809, 869, 916, 926, 1648
NlaIV	GGNNCC	5 cut(s)	159, 367, 1173, 1468, 1486
NmuCI	GTSAC	2 cut(s)	1534, 1640
NruI	TCGCGA	1 cut(s)	41
NsbI	TGCGCA	1 cut(s)	1352
NsiI	ATGCAT	1 cut(s)	814
NspI	RCATGY	1 cut(s)	916
PaeI	GCATGC	1 cut(s)	916
PaqCI	CACCTGC	1 cut(s)	1342
PceI	AGGCCT	2 cut(s)	149, 505
PciSI	GCTCTTC	1 cut(s)	399
PcsI	WCGNNNNNNNCGW	3 cut(s)	45, 1448, 1656
PctI	GAATGC	2 cut(s)	1042, 1284
PdiI	GCCGGC	1 cut(s)	175
PdmI	GAANNNNTTC	1 cut(s)	246
PfeI	GAWTC	2 cut(s)	392, 1370
PflFI	GACNNNGTC	2 cut(s)	114, 1646
PkrI	GCNGC	7 cut(s)	24, 85, 538, 552, 679, 1503, 1617
PleI	GAGTC	5 cut(s)	172, 317, 364, 380, 841
PpsI	GAGTC	5 cut(s)	172, 317, 364, 380, 841
Ppu21I	YACGTR	1 cut(s)	1452
Psp6I	CCWGG	2 cut(s)	907, 1075
PspEI	GGTNACC	1 cut(s)	1067
PspFI	CCCAGC	1 cut(s)	1083
PspGI	CCWGG	2 cut(s)	907, 1075
PspN4I	GGNNCC	5 cut(s)	159, 367, 1173, 1468, 1486
PspOMI	GGGCCC	1 cut(s)	1484
PspPI	GGNCC	3 cut(s)	995, 1484, 1485
PsrI	GAACNNNNNNTAC	2 cut(s)	1125, 1157
PstI	CTGCAG	2 cut(s)	538, 627
PstNI	CAGNNNCTG	1 cut(s)	395
PsuI	RGATCY	1 cut(s)	492
PsyI	GACNNNGTC	2 cut(s)	114, 1646
PvuII	CAGCTG	1 cut(s)	539
RruI	TCGCGA	1 cut(s)	41
RsaI	GTAC	6 cut(s)	585, 824, 863, 1312, 1395, 1589
RsaNI	GTAC	6 cut(s)	584, 823, 862, 1311, 1394, 1588
RseI	CAYNNNNRTG	3 cut(s)	980, 1077, 1197
SapI	GCTCTTC	1 cut(s)	399
SatI	GCNGC	7 cut(s)	23, 84, 537, 551, 678, 1502, 1616
Sau3AI	GATC	6 cut(s)	208, 492, 541, 856, 1153, 1357
Sau96I	GGNCC	3 cut(s)	995, 1484, 1485
ScaI	AGTACT	2 cut(s)	1312, 1395
SchI	GAGTC	5 cut(s)	173, 317, 365, 380, 841
ScrFI	CCNGG	6 cut(s)	909, 1029, 1030, 1053, 1077, 1425
SduI	GDGCHC	3 cut(s)	370, 961, 1488
SfaNI	GCATC	2 cut(s)	517, 799
SfcI	CTRYAG	3 cut(s)	534, 623, 1293
SgrAI	CRCCGGYG	2 cut(s)	173, 1319
SinI	GGWCC	1 cut(s)	995
SmaI	CCCGGG	1 cut(s)	1030
SmiMI	CAYNNNNRTG	3 cut(s)	980, 1077, 1197
SmlI	CTYRAG	1 cut(s)	1306
SmoI	CTYRAG	1 cut(s)	1306
SphI	GCATGC	1 cut(s)	916
SseBI	AGGCCT	2 cut(s)	149, 505
SsiI	CCGC	3 cut(s)	8, 81, 84
SspMI	CTAG	1 cut(s)	1059
StuI	AGGCCT	2 cut(s)	149, 505
StyD4I	CCNGG	6 cut(s)	907, 1027, 1028, 1051, 1075, 1423
TaaI	ACNGT	4 cut(s)	583, 654, 1119, 1573
TaiI	ACGT	5 cut(s)	115, 195, 637, 1454, 1678
TaqI	TCGA	2 cut(s)	95, 303
TaqII	GACCGA	1 cut(s)	197
TatI	WGTACW	2 cut(s)	1310, 1393
TauI	GCSGC	1 cut(s)	86
TfiI	GAWTC	2 cut(s)	392, 1370
TscAI	CASTG	1 cut(s)	1645
TseFI	GTSAC	2 cut(s)	1534, 1640
TseI	GCWGC	6 cut(s)	22, 536, 550, 677, 1501, 1615
Tsp45I	GTSAC	2 cut(s)	1534, 1640
TspDTI	ATGAA	7 cut(s)	432, 485, 661, 882, 917, 939, 1119
TspGWI	ACGGA	2 cut(s)	316, 478
TspMI	CCCGGG	1 cut(s)	1028
TspRI	CASTG	1 cut(s)	1645
Tth111I	GACNNNGTC	2 cut(s)	114, 1646
VpaK11BI	GGWCC	1 cut(s)	995
XapI	RAATTY	5 cut(s)	287, 297, 308, 617, 1023
XceI	RCATGY	1 cut(s)	916
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1121
XmaI	CCCGGG	1 cut(s)	1028
XmiI	GTMKAC	1 cut(s)	1296
XmnI	GAANNNNTTC	1 cut(s)	246
XspI	CTAG	1 cut(s)	1059
ZraI	GACGTC	2 cut(s)	113, 193
ZrmI	AGTACT	2 cut(s)	1312, 1395
Zsp2I	ATGCAT	1 cut(s)	814

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.