Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0004036

Physical Location & Seq

Genomic Coordinates Forward (+)

38496936 .. 38501629

Gene Structure 4694 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1297600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1698 bp

ATGACTGCGGAGGTTCTCGCGCAGCCAAACGGCGCCGTCGCGAACGGCGACCTGAACGGAATCCCTAACAACAACGCCGCCGCCGCCAAGAAGTCGAGGGAGAGCGAGCGACGCCGTCGCAGGAGAAAGCAGAAGAAGAACAACAAAGCCTCTCAGGCGCCGGAGTCCAGCGCCGGCGAGACCGACGATGACGTCAAGGAGAATAATGATACTCAGCAGATTATTGAGCAAGTTCAAATTGAATATGTTCCAGAGAAACCTGAGTTAATTGATGGCATGGATGAGGAATTCAGAAAAATTTTCGAGAAATTCTCCTTCCAGGACTCTGAGGGTGTTGAGGTGGATAAGAAGGATGAGTCTGAAGGAGTCCCCAAAAAGAAGGCTGACTCAGATTCTGAAGAGGAAGAGCAGGATAATGAACAAAAAGAGAAAGGAATATCAAACAAGAAGAAAAAGCTTCAACGGAGAATGAAGATTGCAGAATTGAAACAGATTTGCCAAAGGCCTGATGTTGTTGAGGTGTGGGATGCAACTGCAGCTGATCCTAAGCTGCTGGTGTTTCTAAAATCATATCGCAATACTGTACCCGTGCCAAGACATTGGTGTCAGAAAAGGAAATTTCTGCAGGGTAAGCGTGGTATTGAGAAACAACCGTTTCAGCTTCCAGACTTCATTGCTGCTACAGGAATTGAGAAAATTAGACAGGCGTACATTGAAAAAGAAGATAGCAAGAAGTTGAAGCAAAAACAACGGGAGCGTATGCAACCAAAGATGGGGAAAATGGATATCGATTATCAGGTTCTCCATGATGCATTTTTCAAGTACCAGACTAAGCCAAAGTTGACAACTCTTGGTGATCTGTACCATGAAGGGAAGGAATTTGAGGTGAAATTGAGGGAGATGAAACCAGGCATGCTTTCACATGAACTAAAAGAAGCTCTGGGTATGCCAGATGGTGCTCCTCCCCCATGGCTCATCAATATGCAGAGATATGGTCCTCCACCATCATATCCACATCTGAAAATTCCCGGGCTGAATGCTCCTATCCCTCCTGGAGCCAGCTTTGGTTACCATCCTGGTGGCTGGGGAAAGCCTCCTGTTGATGAATATGGCCAACCGTTGTATGGAGATGTATTTGGCGTTCAGCAACAAGATCAGCCCAATTATGAGGAGCCTGTTGACAAGACAAAGCATTGGGGTGATTTGGAGGAAGAGGAAGAGGAGGAAGAAGAAGAAGAAGAGGAAGAGGAAGAACAGCCTGAGGACGAAGATTTAGAGGATGGCATTCAATCTGTAGACAGCATGTCAAGTACTCCCACTGGCGTTGAGACACCTGCTCAAATTGAACTTCGCAAGGATCAGAAAAAAGAACCTGAAAAGCCTTTATACCAAGTACTTGAACAAAAAGAGGAAAGAATTGCTCCCGGGACACTTCTTGCAACGGGTCACACGTATAATTTGGCTGGCACCCAAGACAAAGCAGGGGCCAAAAAGGTTGAGCTGCTTAGAGGTCAGAAAGCTGATAAAGTGGAGGTCACTATACAACCCGAAGAATTGGATGATATGGAGACCGTTTTGCCTGCAAAGTATGAAGAAGCAAGGGAGGAGGAGAAGCTGCGAAGTCAGAAGGAAGACTTCAGTGACATGGTCGCAGAGGTGTGTGCCTCTTCTCTACCTTTGGCAAATTCTAACAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000375 GO:0000377 GO:0000398 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0005686 GO:0005689 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016604 GO:0016607 GO:0030532 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0070013 GO:0071011 GO:0071013 GO:0071704 GO:0090304 GO:0097525 GO:0120114 GO:1901360 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DUF382 PSP

Protein Analysis

566

Amino Acids

64.4

Weight (kDa)

4.95

Isoelectric Point (pI)

59.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF382	PF04037	167 - 293	9e-60	Domain of unknown function (DUF382)
PSP	PF04046	302 - 347	9.7e-23	PSP

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004036)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11520 AT4G21660 AT4G21660 AT4G21660
fragaria_vesca	FvH4_3g04740 FvH4_6g45300
malus_domestica	MD05G1318200.v1.1 MD10G1297600.v1.1
prunus_persica	Prupe.4G042600_v2.0.a1 Prupe.4G042600_v2.0.a1
pyrus_communis	pycom05g29460 pycom10g25110
rosa_chinensis	RchiOBHm_Chr2g0163041 RchiOBHm_Chr5g0007761
rosa_laevigata	RLG00000021367 RLG00000031486
rosa_multiflora	Rmu_co8286435.1_g000001 Rmu_co8351911.1_g000001 Rmu_sc0005098.1_g000019 Rmu_sc0007039.1_g000007
rosa_roxburghii	Rroxscaffold_1G00068650 Rroxscaffold_2G00087600
rosa_rugosa	Rorug02G0500000.1 Rorug04G0430100
rosa_samantha	Rh2AG566300 Rh2BG578600 Rh2CG548500 Rh5AG058500 Rh5BG055300 Rh5CG065100 Rh5DG054300
rosa_wichuraiana	Rw2G046870 Rw5G006120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1342
AasI	GACNNNNNNGTC	2 cut(s)	191, 603
AatII	GACGTC	1 cut(s)	195
Acc36I	ACCTGC	1 cut(s)	1342
AccB1I	GGYRCC	3 cut(s)	32, 157, 1466
AccI	GTMKAC	1 cut(s)	1296
AccII	CGCG	2 cut(s)	20, 41
AciI	CCGC	4 cut(s)	8, 78, 81, 84
AclWI	GGATC	2 cut(s)	536, 1365
AcoI	YGGCCR	1 cut(s)	1111
AcsI	RAATTY	7 cut(s)	287, 297, 308, 617, 878, 1023, 1684
AcuI	CTGAAG	3 cut(s)	381, 417, 1621
AcyI	GRCGYC	4 cut(s)	33, 112, 158, 192
AfaI	GTAC	6 cut(s)	585, 710, 824, 863, 1312, 1395
AfiI	CCNNNNNNNGG	2 cut(s)	773, 1124
AflIII	ACRYGT	1 cut(s)	1449
AhdI	GACNNNNNGTC	1 cut(s)	1303
AjnI	CCWGG	4 cut(s)	318, 907, 1051, 1075
AluBI	AGCT	9 cut(s)	457, 539, 550, 661, 938, 1062, 1501, 1520, 1615
AluI	AGCT	9 cut(s)	457, 539, 550, 661, 938, 1062, 1501, 1520, 1615
Alw21I	GWGCWC	1 cut(s)	961
Alw26I	GTCTC	3 cut(s)	173, 1322, 1562
AlwI	GGATC	2 cut(s)	536, 1365
AlwNI	CAGNNNCTG	1 cut(s)	395
Ama87I	CYCGRG	2 cut(s)	1028, 1424
AoxI	GGCC	3 cut(s)	503, 1111, 1485
ApeKI	GCWGC	6 cut(s)	22, 536, 550, 677, 1501, 1615
ApoI	RAATTY	7 cut(s)	287, 297, 308, 617, 878, 1023, 1684
Asp700I	GAANNNNTTC	1 cut(s)	246
AspLEI	GCGC	4 cut(s)	22, 35, 160, 173
AspS9I	GGNCC	2 cut(s)	995, 1485
AsuC2I	CCSGG	4 cut(s)	1029, 1030, 1425, 1426
AsuHPI	GGTGA	3 cut(s)	866, 898, 1211
AvaI	CYCGRG	2 cut(s)	1028, 1424
AvaII	GGWCC	1 cut(s)	995
AxyI	CCTNAGG	1 cut(s)	1260
BalI	TGGCCA	1 cut(s)	1113
BanI	GGYRCC	3 cut(s)	32, 157, 1466
BbsI	GAAGAC	1 cut(s)	1638
Bbv12I	GWGCWC	1 cut(s)	961
BbvI	GCAGC	6 cut(s)	34, 537, 548, 664, 1488, 1602
BccI	CCATC	6 cut(s)	266, 766, 947, 1012, 1080, 1274
BceAI	ACGGC	4 cut(s)	20, 46, 61, 99
BciT130I	CCWGG	4 cut(s)	320, 909, 1053, 1077
BcnI	CCSGG	4 cut(s)	1029, 1030, 1425, 1426
BcoDI	GTCTC	3 cut(s)	173, 1322, 1562
BfmI	CTRYAG	4 cut(s)	534, 623, 681, 1293
BfoI	RGCGCY	3 cut(s)	36, 161, 174
BfuAI	ACCTGC	1 cut(s)	1342
BglI	GCCNNNNNGGC	1 cut(s)	155
BisI	GCNGC	9 cut(s)	23, 78, 81, 84, 537, 551, 678, 1502, 1616
BlsI	GCNGC	9 cut(s)	24, 79, 82, 85, 538, 552, 679, 1503, 1617
BmcAI	AGTACT	2 cut(s)	1312, 1395
Bme1390I	CCNGG	8 cut(s)	320, 909, 1029, 1030, 1053, 1077, 1425, 1426
Bme18I	GGWCC	1 cut(s)	995
BmeRI	GACNNNNNGTC	1 cut(s)	1303
BmeT110I	CYCGRG	2 cut(s)	1028, 1424
BmgT120I	GGNCC	2 cut(s)	995, 1485
BmiI	GGNNCC	6 cut(s)	34, 159, 1057, 1173, 1468, 1486
BmrFI	CCNGG	8 cut(s)	320, 909, 1029, 1030, 1053, 1077, 1425, 1426
BmsI	GCATC	2 cut(s)	517, 799
BpiI	GAAGAC	1 cut(s)	1638
BplI	GAGNNNNNCTC	2 cut(s)	296, 328
BpmI	CTGGAG	1 cut(s)	1074
Bpu10I	CCTNAGC	1 cut(s)	546
BpuMI	CCSGG	4 cut(s)	1029, 1030, 1425, 1426
Bsa29I	ATCGAT	1 cut(s)	789
BsaAI	YACGTR	1 cut(s)	1452
BsaHI	GRCGYC	4 cut(s)	33, 112, 158, 192
BsaI	GGTCTC	2 cut(s)	173, 1562
BsaJI	CCNNGG	3 cut(s)	968, 1028, 1424
Bsc4I	CCNNNNNNNGG	2 cut(s)	773, 1124
Bse118I	RCCGGY	1 cut(s)	173
Bse1I	ACTGG	1 cut(s)	1324
Bse21I	CCTNAGG	1 cut(s)	1260
Bse3DI	GCAATG	1 cut(s)	672
BseBI	CCWGG	4 cut(s)	320, 909, 1053, 1077
BseCI	ATCGAT	1 cut(s)	789
BseDI	CCNNGG	3 cut(s)	968, 1028, 1424
BseGI	GGATG	6 cut(s)	286, 358, 532, 1072, 1285, 1564
BseLI	CCNNNNNNNGG	2 cut(s)	773, 1124
BseMI	GCAATG	1 cut(s)	672
BseMII	CTCAG	6 cut(s)	167, 227, 252, 318, 402, 1251
BseNI	ACTGG	1 cut(s)	1324
BseRI	GAGGAG	5 cut(s)	951, 1184, 1235, 1619, 1622
BseXI	GCAGC	6 cut(s)	34, 537, 548, 664, 1488, 1602
BseYI	CCCAGC	1 cut(s)	1083
Bsh1236I	CGCG	2 cut(s)	20, 41
BshFI	GGCC	3 cut(s)	505, 1113, 1487
BshNI	GGYRCC	3 cut(s)	32, 157, 1466
BshVI	ATCGAT	1 cut(s)	789
BsiHKAI	GWGCWC	1 cut(s)	961
BsiHKCI	CYCGRG	2 cut(s)	1028, 1424
BsiSI	CCGG	4 cut(s)	161, 174, 1029, 1425
BslFI	GGGAC	2 cut(s)	353, 1441
BslI	CCNNNNNNNGG	2 cut(s)	773, 1124
BsmAI	GTCTC	3 cut(s)	173, 1322, 1562
BsmFI	GGGAC	2 cut(s)	353, 1441
BsmI	GAATGC	2 cut(s)	1042, 1284
BsnI	GGCC	3 cut(s)	505, 1113, 1487
Bso31I	GGTCTC	2 cut(s)	173, 1562
BsoBI	CYCGRG	2 cut(s)	1028, 1424
Bsp1286I	GDGCHC	1 cut(s)	961
Bsp143I	GATC	4 cut(s)	541, 856, 1153, 1357
Bsp19I	CCATGG	1 cut(s)	968
Bsp68I	TCGCGA	1 cut(s)	41
BspACI	CCGC	4 cut(s)	8, 78, 81, 84
BspANI	GGCC	3 cut(s)	505, 1113, 1487
BspCNI	CTCAG	6 cut(s)	166, 226, 253, 319, 401, 1252
BspDI	ATCGAT	1 cut(s)	789
BspFNI	CGCG	2 cut(s)	20, 41
BspLI	GGNNCC	6 cut(s)	34, 159, 1057, 1173, 1468, 1486
BspMAI	CTGCAG	2 cut(s)	538, 627
BspMI	ACCTGC	1 cut(s)	1342
BspPI	GGATC	2 cut(s)	536, 1365
BspQI	GCTCTTC	1 cut(s)	399
BspT107I	GGYRCC	3 cut(s)	32, 157, 1466
BspTNI	GGTCTC	2 cut(s)	173, 1562
BsrDI	GCAATG	1 cut(s)	672
BsrFI	RCCGGY	1 cut(s)	173
BsrI	ACTGG	1 cut(s)	1324
BssAI	RCCGGY	1 cut(s)	173
BssECI	CCNNGG	3 cut(s)	968, 1028, 1424
BssMI	GATC	4 cut(s)	541, 856, 1153, 1357
BssNI	GRCGYC	4 cut(s)	33, 112, 158, 192
BssT1I	CCWWGG	1 cut(s)	968
Bst2UI	CCWGG	4 cut(s)	320, 909, 1053, 1077
Bst4CI	ACNGT	4 cut(s)	583, 654, 1119, 1573
Bst6I	CTCTTC	7 cut(s)	393, 399, 1206, 1212, 1233, 1239, 1672
BstACI	GRCGYC	4 cut(s)	33, 112, 158, 192
BstBAI	YACGTR	1 cut(s)	1452
BstC8I	GCNNGC	6 cut(s)	107, 175, 914, 1060, 1465, 1581
BstDEI	CTNAG	9 cut(s)	153, 213, 261, 327, 388, 546, 831, 1260, 1505
BstDSI	CCRYGG	1 cut(s)	968
BstEII	GGTNACC	1 cut(s)	1067
BstF5I	GGATG	6 cut(s)	286, 358, 532, 1072, 1285, 1564
BstFNI	CGCG	2 cut(s)	20, 41
BstH2I	RGCGCY	3 cut(s)	36, 161, 174
BstHHI	GCGC	4 cut(s)	22, 35, 160, 173
BstKTI	GATC	4 cut(s)	544, 859, 1156, 1360
BstMAI	GTCTC	3 cut(s)	173, 1322, 1562
BstMBI	GATC	4 cut(s)	541, 856, 1153, 1357
BstMWI	GCNNNNNNNGC	5 cut(s)	83, 111, 155, 536, 631
BstNI	CCWGG	4 cut(s)	320, 909, 1053, 1077
BstNSI	RCATGY	2 cut(s)	916, 1306
BstPI	GGTNACC	1 cut(s)	1067
BstSCI	CCNGG	8 cut(s)	318, 907, 1027, 1028, 1051, 1075, 1423, 1424
BstSFI	CTRYAG	4 cut(s)	534, 623, 681, 1293
BstUI	CGCG	2 cut(s)	20, 41
BstV1I	GCAGC	6 cut(s)	34, 537, 548, 664, 1488, 1602
BstV2I	GAAGAC	1 cut(s)	1638
BstXI	CCANNNNNNTGG	2 cut(s)	600, 1079
Bsu15I	ATCGAT	1 cut(s)	789
Bsu36I	CCTNAGG	1 cut(s)	1260
BsuRI	GGCC	3 cut(s)	505, 1113, 1487
BsuTUI	ATCGAT	1 cut(s)	789
BtgI	CCRYGG	1 cut(s)	968
BtsCI	GGATG	6 cut(s)	286, 358, 532, 1072, 1285, 1564
BtsIMutI	CAGTG	2 cut(s)	1317, 1645
BtuMI	TCGCGA	1 cut(s)	41
BveI	ACCTGC	1 cut(s)	1342
Cac8I	GCNNGC	6 cut(s)	107, 175, 914, 1060, 1465, 1581
CaiI	CAGNNNCTG	1 cut(s)	395
CfoI	GCGC	4 cut(s)	22, 35, 160, 173
Cfr10I	RCCGGY	1 cut(s)	173
Cfr13I	GGNCC	2 cut(s)	995, 1485
Cfr9I	CCCGGG	2 cut(s)	1028, 1424
ClaI	ATCGAT	1 cut(s)	789
CseI	GACGC	1 cut(s)	120
Csp6I	GTAC	6 cut(s)	584, 709, 823, 862, 1311, 1394
CspCI	CAANNNNNGTGG	2 cut(s)	1306, 1341
CviAII	CATG	8 cut(s)	277, 806, 866, 913, 923, 969, 1303, 1645
CviQI	GTAC	6 cut(s)	584, 709, 823, 862, 1311, 1394
DdeI	CTNAG	9 cut(s)	153, 213, 261, 327, 388, 546, 831, 1260, 1505
DinI	GGCGCC	2 cut(s)	34, 159
DpnI	GATC	4 cut(s)	543, 858, 1155, 1359
DpnII	GATC	4 cut(s)	541, 856, 1153, 1357
DrdI	GACNNNNNNGTC	2 cut(s)	191, 603
DriI	GACNNNNNGTC	1 cut(s)	1303
DseDI	GACNNNNNNGTC	2 cut(s)	191, 603
EaeI	YGGCCR	1 cut(s)	1111
Eam1104I	CTCTTC	7 cut(s)	393, 399, 1206, 1212, 1233, 1239, 1672
Eam1105I	GACNNNNNGTC	1 cut(s)	1303
EarI	CTCTTC	7 cut(s)	393, 399, 1206, 1212, 1233, 1239, 1672
Eco130I	CCWWGG	1 cut(s)	968
Eco147I	AGGCCT	1 cut(s)	505
Eco31I	GGTCTC	2 cut(s)	173, 1562
Eco32I	GATATC	1 cut(s)	787
Eco47I	GGWCC	1 cut(s)	995
Eco57I	CTGAAG	3 cut(s)	381, 417, 1621
Eco81I	CCTNAGG	1 cut(s)	1260
Eco88I	CYCGRG	2 cut(s)	1028, 1424
Eco91I	GGTNACC	1 cut(s)	1067
EcoO65I	GGTNACC	1 cut(s)	1067
EcoRI	GAATTC	1 cut(s)	287
EcoRII	CCWGG	4 cut(s)	318, 907, 1051, 1075
EcoRV	GATATC	1 cut(s)	787
EcoT14I	CCWWGG	1 cut(s)	968
EcoT22I	ATGCAT	1 cut(s)	814
EgeI	GGCGCC	2 cut(s)	34, 159
EheI	GGCGCC	2 cut(s)	34, 159
ErhI	CCWWGG	1 cut(s)	968
FaeI	CATG	8 cut(s)	280, 809, 869, 916, 926, 972, 1306, 1648
FalI	AAGNNNNNCTT	2 cut(s)	1619, 1651
FaqI	GGGAC	2 cut(s)	353, 1441
FatI	CATG	8 cut(s)	276, 805, 865, 912, 922, 968, 1302, 1644
FblI	GTMKAC	1 cut(s)	1296
Fnu4HI	GCNGC	9 cut(s)	23, 78, 81, 84, 537, 551, 678, 1502, 1616
FokI	GGATG	6 cut(s)	293, 365, 539, 1059, 1292, 1571
Fsp4HI	GCNGC	9 cut(s)	23, 78, 81, 84, 537, 551, 678, 1502, 1616
GlaI	GCGC	4 cut(s)	21, 34, 159, 172
GluI	GCNGC	9 cut(s)	23, 78, 81, 84, 537, 551, 678, 1502, 1616
GsaI	CCCAGC	1 cut(s)	1087
GsuI	CTGGAG	1 cut(s)	1074
HaeII	RGCGCY	3 cut(s)	36, 161, 174
HaeIII	GGCC	3 cut(s)	505, 1113, 1487
HapII	CCGG	4 cut(s)	161, 174, 1029, 1425
HgaI	GACGC	1 cut(s)	120
HhaI	GCGC	4 cut(s)	22, 35, 160, 173
Hin1I	GRCGYC	4 cut(s)	33, 112, 158, 192
Hin1II	CATG	8 cut(s)	280, 809, 869, 916, 926, 972, 1306, 1648
Hin6I	GCGC	4 cut(s)	20, 33, 158, 171
HinP1I	GCGC	4 cut(s)	20, 33, 158, 171
HincII	GTYRAC	2 cut(s)	843, 1180
HindII	GTYRAC	2 cut(s)	843, 1180
HindIII	AAGCTT	1 cut(s)	455
HinfI	GANTC	7 cut(s)	60, 164, 323, 356, 366, 386, 392
HpaII	CCGG	4 cut(s)	161, 174, 1029, 1425
HphI	GGTGA	3 cut(s)	866, 898, 1211
Hpy166II	GTNNAC	3 cut(s)	843, 1180, 1297
Hpy188III	TCNNGA	4 cut(s)	40, 251, 304, 665
Hpy8I	GTNNAC	3 cut(s)	843, 1180, 1297
Hpy99I	CGWCG	4 cut(s)	41, 114, 120, 188
HpyAV	CCTTC	7 cut(s)	325, 343, 356, 373, 863, 868, 1621
HpyCH4III	ACNGT	4 cut(s)	583, 654, 1119, 1573
HpyCH4IV	ACGT	2 cut(s)	192, 1451
HpyCH4V	TGCA	9 cut(s)	479, 530, 536, 625, 763, 812, 985, 1439, 1583
HpyF10VI	GCNNNNNNNGC	5 cut(s)	83, 111, 155, 536, 631
HpyF3I	CTNAG	9 cut(s)	153, 213, 261, 327, 388, 546, 831, 1260, 1505
HpySE526I	ACGT	2 cut(s)	192, 1451
Hsp92I	GRCGYC	4 cut(s)	33, 112, 158, 192
Hsp92II	CATG	8 cut(s)	280, 809, 869, 916, 926, 972, 1306, 1648
HspAI	GCGC	4 cut(s)	20, 33, 158, 171
KasI	GGCGCC	2 cut(s)	32, 157
KroI	GCCGGC	1 cut(s)	173
KroNI	GCCGGC	1 cut(s)	175
Kzo9I	GATC	4 cut(s)	541, 856, 1153, 1357
LguI	GCTCTTC	1 cut(s)	399
LmnI	GCTCC	6 cut(s)	754, 964, 1045, 1055, 1171, 1426
Lsp1109I	GCAGC	6 cut(s)	34, 537, 548, 664, 1488, 1602
LweI	GCATC	2 cut(s)	517, 799
MaeII	ACGT	2 cut(s)	192, 1451
MaeIII	GTNAC	4 cut(s)	1067, 1445, 1534, 1640
MalI	GATC	4 cut(s)	543, 858, 1155, 1359
MboI	GATC	4 cut(s)	541, 856, 1153, 1357
MhlI	GDGCHC	1 cut(s)	961
MlsI	TGGCCA	1 cut(s)	1113
MluNI	TGGCCA	1 cut(s)	1113
Mly113I	GGCGCC	2 cut(s)	33, 158
MlyI	GAGTC	5 cut(s)	173, 317, 365, 375, 380
Mox20I	TGGCCA	1 cut(s)	1113
Mph1103I	ATGCAT	1 cut(s)	814
MreI	CGCCGGCG	1 cut(s)	173
MroNI	GCCGGC	1 cut(s)	173
MroXI	GAANNNNTTC	1 cut(s)	246
MscI	TGGCCA	1 cut(s)	1113
MseI	TTAA	1 cut(s)	266
MslI	CAYNNNNRTG	3 cut(s)	980, 1077, 1197
Msp20I	TGGCCA	1 cut(s)	1113
MspA1I	CMGCKG	1 cut(s)	539
MspI	CCGG	4 cut(s)	161, 174, 1029, 1425
MspR9I	CCNGG	8 cut(s)	320, 909, 1029, 1030, 1053, 1077, 1425, 1426
Mva1269I	GAATGC	2 cut(s)	1042, 1284
MvaI	CCWGG	4 cut(s)	320, 909, 1053, 1077
MvnI	CGCG	2 cut(s)	20, 41
MwoI	GCNNNNNNNGC	5 cut(s)	83, 111, 155, 536, 631
NaeI	GCCGGC	1 cut(s)	175
NarI	GGCGCC	2 cut(s)	33, 158
NciI	CCSGG	4 cut(s)	1029, 1030, 1425, 1426
NcoI	CCATGG	1 cut(s)	968
NdeII	GATC	4 cut(s)	541, 856, 1153, 1357
NgoMIV	GCCGGC	1 cut(s)	173
NlaIII	CATG	8 cut(s)	280, 809, 869, 916, 926, 972, 1306, 1648
NlaIV	GGNNCC	6 cut(s)	34, 159, 1057, 1173, 1468, 1486
NmuCI	GTSAC	3 cut(s)	1445, 1534, 1640
NruI	TCGCGA	1 cut(s)	41
NsiI	ATGCAT	1 cut(s)	814
NspI	RCATGY	2 cut(s)	916, 1306
PaeI	GCATGC	1 cut(s)	916
PaqCI	CACCTGC	1 cut(s)	1342
PceI	AGGCCT	1 cut(s)	505
PciSI	GCTCTTC	1 cut(s)	399
PcsI	WCGNNNNNNNCGW	2 cut(s)	45, 1448
PctI	GAATGC	2 cut(s)	1042, 1284
PdiI	GCCGGC	1 cut(s)	175
PdmI	GAANNNNTTC	1 cut(s)	246
PfeI	GAWTC	2 cut(s)	60, 392
PflFI	GACNNNGTC	2 cut(s)	114, 1646
PfoI	TCCNGGA	2 cut(s)	318, 1051
PkrI	GCNGC	9 cut(s)	24, 79, 82, 85, 538, 552, 679, 1503, 1617
PleI	GAGTC	5 cut(s)	172, 317, 364, 374, 380
PluTI	GGCGCC	2 cut(s)	36, 161
PpsI	GAGTC	5 cut(s)	172, 317, 364, 374, 380
Ppu21I	YACGTR	1 cut(s)	1452
Psp6I	CCWGG	4 cut(s)	318, 907, 1051, 1075
PspEI	GGTNACC	1 cut(s)	1067
PspFI	CCCAGC	1 cut(s)	1083
PspGI	CCWGG	4 cut(s)	318, 907, 1051, 1075
PspN4I	GGNNCC	6 cut(s)	34, 159, 1057, 1173, 1468, 1486
PspPI	GGNCC	2 cut(s)	995, 1485
PsrI	GAACNNNNNNTAC	2 cut(s)	1125, 1157
PstI	CTGCAG	2 cut(s)	538, 627
PstNI	CAGNNNCTG	1 cut(s)	395
PsyI	GACNNNGTC	2 cut(s)	114, 1646
PvuII	CAGCTG	1 cut(s)	539
RruI	TCGCGA	1 cut(s)	41
RsaI	GTAC	6 cut(s)	585, 710, 824, 863, 1312, 1395
RsaNI	GTAC	6 cut(s)	584, 709, 823, 862, 1311, 1394
RseI	CAYNNNNRTG	3 cut(s)	980, 1077, 1197
SapI	GCTCTTC	1 cut(s)	399
SaqAI	TTAA	1 cut(s)	266
SatI	GCNGC	9 cut(s)	23, 78, 81, 84, 537, 551, 678, 1502, 1616
Sau3AI	GATC	4 cut(s)	541, 856, 1153, 1357
Sau96I	GGNCC	2 cut(s)	995, 1485
ScaI	AGTACT	2 cut(s)	1312, 1395
SchI	GAGTC	5 cut(s)	173, 317, 365, 375, 380
ScrFI	CCNGG	8 cut(s)	320, 909, 1029, 1030, 1053, 1077, 1425, 1426
SduI	GDGCHC	1 cut(s)	961
SfaNI	GCATC	2 cut(s)	517, 799
SfcI	CTRYAG	4 cut(s)	534, 623, 681, 1293
SfoI	GGCGCC	2 cut(s)	34, 159
SgrAI	CRCCGGYG	1 cut(s)	173
SinI	GGWCC	1 cut(s)	995
SmaI	CCCGGG	2 cut(s)	1030, 1426
SmiMI	CAYNNNNRTG	3 cut(s)	980, 1077, 1197
SphI	GCATGC	1 cut(s)	916
SseBI	AGGCCT	1 cut(s)	505
SsiI	CCGC	4 cut(s)	8, 78, 81, 84
SspDI	GGCGCC	2 cut(s)	32, 157
StuI	AGGCCT	1 cut(s)	505
StyD4I	CCNGG	8 cut(s)	318, 907, 1027, 1028, 1051, 1075, 1423, 1424
StyI	CCWWGG	1 cut(s)	968
TaaI	ACNGT	4 cut(s)	583, 654, 1119, 1573
TaiI	ACGT	2 cut(s)	195, 1454
TaqI	TCGA	3 cut(s)	95, 303, 789
TaqII	GACCGA	1 cut(s)	197
TatI	WGTACW	2 cut(s)	1310, 1393
TauI	GCSGC	3 cut(s)	80, 83, 86
TfiI	GAWTC	2 cut(s)	60, 392
Tru1I	TTAA	1 cut(s)	266
Tru9I	TTAA	1 cut(s)	266
TscAI	CASTG	2 cut(s)	1324, 1645
TseFI	GTSAC	3 cut(s)	1445, 1534, 1640
TseI	GCWGC	6 cut(s)	22, 536, 550, 677, 1501, 1615
Tsp45I	GTSAC	3 cut(s)	1445, 1534, 1640
TspDTI	ATGAA	8 cut(s)	432, 485, 661, 882, 917, 939, 1119, 1605
TspGWI	ACGGA	2 cut(s)	72, 478
TspMI	CCCGGG	2 cut(s)	1028, 1424
TspRI	CASTG	2 cut(s)	1324, 1645
Tth111I	GACNNNGTC	2 cut(s)	114, 1646
VpaK11BI	GGWCC	1 cut(s)	995
XapI	RAATTY	7 cut(s)	287, 297, 308, 617, 878, 1023, 1684
XceI	RCATGY	2 cut(s)	916, 1306
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1121
XmaI	CCCGGG	2 cut(s)	1028, 1424
XmiI	GTMKAC	1 cut(s)	1296
XmnI	GAANNNNTTC	1 cut(s)	246
ZraI	GACGTC	1 cut(s)	193
ZrmI	AGTACT	2 cut(s)	1312, 1395
Zsp2I	ATGCAT	1 cut(s)	814

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.