MD05G1322200.v1.1

SNF2 family N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004421
Physical Location & Seq
Reverse (-)
44953958 .. 44962448
8491 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1322200.v1.1.491

Sequence Viewer

Length: 4164 bp
ATGTTGAAGGCGGAGGACTGCTCGAATTGGAACGAGTTACCGGCCGCCGATGGATTTGCAGGAGAGGAATTCGACGATGACGGCGACGAGATGTGCATAGATGTCGACACGCTCTATCGCCTTCTCGATGAAGAACCCACGAGTTGTCCAGAGAATTTGTCATTGGAAAATCGATTGGAAGCTGAAGCCGTTTCCGATGCTCAACTCCTAAGCGGATCTCAAGCAGTAAAAGAAGAACCGCCTTTAGAAACAAATTTAATGGGTCCAGGCTCATGGAATCCTCCATACAGATCGGAAGCTTCTCATTATGGGGCAGGGGGTCAAGATGGCTCTTTTGATTATGAAGGGAACCATGACATGAATTTTGATTACAAAGAAATGCAACCATCGTCACACACTGGTTCTCCCGGGCATACGTACTCTGCAAGTCTTAAAGACTGGATTTCCTTGATTCCAGCCAACGAGACTTGCTTTACAGAGAGGGTTGCAGTTTCACAGCCATGTAGCACGGCATCTAGTTTTTCTGAAGGGCATGTTAATCACATTTTGAACCACAGAGATCTGAATGTCTTGCAGGGGGAAGTTGATGTGCCAGGGAAAGCTGAATCTGAATATGCTTCACAATGCCTTGGTATTGAGAACATGGACTTAAATTCTAGAACCTATTTCAATTTGGGGCAAAATACCACGGAAACCTTAGGGCTATCTGAAAATAATTCCTGCACTTCTATGGAAATGTCCTCTACCCTCTGTCAAAATTCTGATGTTTTCAGCGACCCTTATACAGCTATGTGTGGCAGGAGCTTAGATAATACATATTTCACTGATGCCTTAATGCATCATAGTCCTACTAGGTATTATTTTCCGAGTAACGAAGAAATGATGCCTAATGTGAAGGATGAATCAGGGGAGTTTCCAACTGATAGTTCATGCTCAACTACCAAGATGAATTTAAATGGCCAGGAGGGGATAACTGTTAAATCTGTTTTTGAGCCTTCAATGATTGATGTTTTAAATGGAAACCAGTGGAATTTTGGATACGGTAATTGTATGTCAGCAATCAGTGGAAATTCTTCTTTTGATGCTGACTCGTTTCCTGCTGACAACAAGGCATCAGTTGAGCCTTTGTGTTCTAACCAGACATACATGTCAAGCAAAAAGGAAATGAATGGTTTGAAAAGTGAAAAGATTGATGAGTTTGTTGCACCAAGTAGCGTTATGCGTCAATCTTTTCGAGGCATGGATGAAGCTGTCAGTAGACAGTCTTATAGTGCTGATGATCACTTTTTTGACAAAGACTCAAAGCAGTCTGGTTTTGGTATATCAACTCAGAATCTTGGTAACTCAGTGGATGATGATGAAGTCATGATTGCTGCATGTAAGAGAGCTTGTCACTCTCAAGATTTTAGGAATGGAAGTTCCACATCCCCTGTTGATGGTCAATCTGTGAATTCAAATGCCTATTTTCCAGGTGCTCAGCCATTCACTTCATCTAGGAATCAGGGTTACATGAACGACAAAGTAACTCATTCTAAGAGCATGCATTTTTCGAAAGTCAGCCCTGAATCAATTCATAGTAACTTCTCTGACAAATCCCCTGCAGAGGATGATGTTGATGTATGTATAATTGAAGATAACAGTCATCCTGCACCCTCAAATCGCTCTTCAGTGGTTAGCAATACTAGTTACGCTGCTCCCGTGTATCAGTCTCCAGCACTTGGAAATACTGTTGTTAATTCGCAACAATTGACGCCGAGTGGTCATTACTCAGGAGTGGGAGGCATGAGATTCAAGACACGAGATGAACAATTGATTTTGAGAGCAGCGTTGCAGGACCTTTCACAGCCCAAGTCAGAAGCTATTCCACCGGATGGACTTTTAGCAGTTCCTCTGTTACGGCACCAGAGAATTGCATTGTCATGGATGGTTCAGAAAGAGACAGCAAGTTTGCACTGTTCTGGAGGAATTCTTGCTGATGATCAGGGATTGGGAAAGACAATATCAACAATTGCACTTATACTTAAGGAAAGGCCTTCATCTGGAGCTTGTCAAGATGTAAAAATGCACAAGCTGGAAACTCTGGATTTGGACAATGACGATGATATGCCTTTTGAGATTGGTGGAAAGAAGCAACATGCTGATGCCCATGAAGTTATTTCAAATGGAACTCCAAATGAGAGCATAAAACCTTTGATACAAGCAAAGGGAAGGCCAGCTGCTGGAACACTTGTTGTTTGTCCCACGAGTGTCCTCCGGCAATGGGCTGAGGAGCTGCAGAACAAGGTAACTGAAAAAGCTAATCTCTCGGTGCTAGTTTACCATGGATGTAACCGGACAAGGGATCCTTTGGAACTGGCTAAGCATGATGTTGTTCTCACAACATATTCAATTGTCAGCATGGAGGTTCCTAAGCAGCCTCTTGTTGATGACGACGATGAGGAGAAAGGAAAGCGAGATGAGTACGATGGTCCACATTACAGTAAGAAAAGGAAATACCCTCAAAATTCTGCAAATAAAGGCTCAAAAGGTAAAAAGGGATTAGAGAGTGCTGTGCTCGATTCTGTTGCTCGCCCTCTTGCTAAGGTTGCATGGTTTAGGGTTGTTCTGGATGAGGCTCAGAGTATTAAGAATCACAGAACTCAAGTAGCTAGGGCCTGTTGGGGTCTTCGTGCGAAACGTAGGTGGTGCTTATCAGGGACTCCTATCCAGAATGCAATTGATGACCTTTATAGCTACTTCAGATTTCTAAAATACGACCCTTATGCTGTATACAAGTCATTTTGCTCTACAATTAAGGTCCCAATCAATAGGAATCCAACAAAAGGGTACAGAAAACTTCAAGCTGTTCTGAAGACAATTATGTTACGCCGCACTAAAGGCACACTTCTTGACGGAGAACCTATTATTACTTTACCACCTAAATCTATAGAATTGAAAAGGGTGGAGTTTTCGAAGGAAGAACGGGATTTCTACTCGAGGCTAGAGGCTGATTCACGTGCTCAGTTTGAAGAATATGCAGCTGCTGGAACTGTTAAACAAAATTATGTTAACATTTTGTTGATGCTTTTGCGTCTTCGACAAGCTTGTGATCACCCTCTTCTTGTTAGACGGTATGATTCAAATTCTTTATGGAGATCTTCAGTTGAGAACGCAAAGAAACTTCCTCGTGATAAACAACAATCCCTTCTGAATTGTTTAGAAGCTTCTTTGGCAATATGCAGCATCTGCAATGATCCGCCTGAAGATGCTGTTGTTTCCGAATGTGGTCATGTCTTCTGTAGCCAATGCATTAATGAACATCTTACTGGCGATGACAACCAGTGCCCAAGCACAAATTGCAAAGTTCGACTCCACGCTTCTTCAGTGTTTTCAAAAGCCACTTTGAACAGTTCTCTTTCTGACCAGACTAATCCAGAAAGCATTGGTTCTGAAGTTTTTGATGCTGTAGAGACTTTCTATGAGGATCGCTCGTACAACTCTTCCAAGATTAAGGCTGCCCTTGACGTTCTCTGCGCAATGTGTAAACCACAGGGTTGTATTTCTGGAAATAGTTGCATAGAAGACACAGTTGATAGAAATGCCAACTCTTCCGAAAAGTCTTCTGATATTTGTGTGGTTGAACCACCTGAGGACGTTCCTAAGAGACAGAACTTGGATTTGGTGACTAATCCTCAGAATTCAAAGAAGGTAGTTAGAGAAAAAGCAATAGTCTTTTCACAGTGGACGAGGATGCTGGACTTGCTTGAAGATTGTCTTAAAACATCTTCCATTGAGTACAGAAGACTCGATGGAACAATGTCAGTAACAGCTAGGGATAAAGCTGTCAAGGATTTTAACACCCTTCCAGAGGTGACAGTCATGATAATGTCTTTGAAAGCAGCTAGTCTTGGTCTAAACATGGTTGCAGCTTGTCATGTTCTTCTTTTGGACCTCTGGTGGAATCCTACCACAGAAGATCAAGCAATTGATAGAGCTCATCGGATTGGGCAGACTCGTCCTGTTACAGTTTTGCGTTTGACCGTTAGAGATACTGTCGAAGATCGAATTTTAGCCCTTCAGCAAAAGAAGAGAGAGATGGTATCGTCTGCGTTTGGAGAGGATAAAACAGGTGGTGGACAGACTCGCCTGACGGTAGAGGATCTCAAGTACCTGTTTATGATGTGA

Protein Analysis

1388

Amino Acids

153.65

Weight (kDa)

5.22

Isoelectric Point (pI)

52.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNF2-rel_dom PF00176 634 - 1032 4.1e-65 SNF2-related domain
zf-C3HC4_3 PF13920 1072 - 1110 8.6e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 1073 - 1109 5.4e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 1073 - 1109 1e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 1073 - 1109 7.8e-06 RING-type zinc-finger
Helicase_C PF00271 1231 - 1329 6.6e-20 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1147
Acc16I TGCGCA 1 cut(s) 3514
AccB1I GGYRCC 1 cut(s) 1899
AccB7I CCANNNNNTGG 3 cut(s) 1718, 1871, 2219
AccI GTMKAC 3 cut(s) 105, 1258, 2769
AciI CCGC 6 cut(s) 11, 45, 213, 239, 2869, 3236
AclWI GGATC 6 cut(s) 223, 2336, 2349, 3227, 3471, 4146
AcoI YGGCCR 2 cut(s) 42, 958
AcvI CACGTG 1 cut(s) 2996
AcyI GRCGYC 1 cut(s) 1751
AfaI GTAC 6 cut(s) 419, 2462, 2828, 3473, 3776, 4148
AflII CTTAAG 1 cut(s) 2021
AflIII ACRYGT 1 cut(s) 1146
AhlI ACTAGT 1 cut(s) 1682
AjnI CCWGG 4 cut(s) 265, 592, 960, 1468
AjuI GAANNNNNNNTTGG 6 cut(s) 146, 178, 910, 942, 3641, 3673
AloI GAACNNNNNNTCC 2 cut(s) 1402, 1434
Alw21I GWGCWC 4 cut(s) 1477, 2556, 3001, 3976
Alw26I GTCTC 5 cut(s) 458, 1713, 1931, 3443, 3637
AlwI GGATC 6 cut(s) 223, 2336, 2349, 3227, 3471, 4146
AlwNI CAGNNNCTG 3 cut(s) 2219, 3023, 3225
Ama87I CYCGRG 2 cut(s) 407, 2974
AoxI GGCC 5 cut(s) 42, 958, 2030, 2210, 2652
ArsI GACNNNNNNTTYG 4 cut(s) 347, 379, 1930, 1962
AseI ATTAAT 1 cut(s) 3291
Asp700I GAANNNNTTC 3 cut(s) 1072, 1569, 1860
AspLEI GCGC 1 cut(s) 3515
AspS9I GGNCC 6 cut(s) 263, 1834, 2468, 2652, 2797, 3928
AsuC2I CCSGG 2 cut(s) 408, 409
AsuHPI GGTGA 3 cut(s) 3083, 3673, 3862
AsuII TTCGAA 2 cut(s) 1550, 2951
AvaI CYCGRG 2 cut(s) 407, 2974
AvaII GGWCC 5 cut(s) 263, 1834, 2468, 2797, 3928
AxyI CCTNAGG 2 cut(s) 697, 3627
BaeGI GKGCMC 1 cut(s) 3326
BalI TGGCCA 1 cut(s) 960
BamHI GGATCC 1 cut(s) 2341
BanI GGYRCC 1 cut(s) 1899
BanII GRGCYC 1 cut(s) 3976
BauI CACGAG 4 cut(s) 139, 1797, 2242, 3165
BbrPI CACGTG 1 cut(s) 2996
BbsI GAAGAC 7 cut(s) 2657, 2858, 3065, 3265, 3567, 3591, 3787
Bbv12I GWGCWC 4 cut(s) 1477, 2556, 3001, 3976
BbvCI CCTCAGC 1 cut(s) 2265
BccI CCATC 9 cut(s) 44, 320, 394, 1430, 1865, 1918, 2459, 3782, 4069
BceAI ACGGC 4 cut(s) 97, 173, 525, 1913
BcgI CGANNNNNNTGC 8 cut(s) 38, 72, 85, 119, 2546, 2580, 2744, 2778
BciT130I CCWGG 4 cut(s) 267, 594, 962, 1470
BciVI GTATCC 1 cut(s) 1031
BclI TGATCA 3 cut(s) 1279, 1978, 3088
BcnI CCSGG 2 cut(s) 408, 409
BcoDI GTCTC 5 cut(s) 458, 1713, 1931, 3443, 3637
BcuI ACTAGT 1 cut(s) 1682
BfmI CTRYAG 5 cut(s) 1599, 2273, 2925, 3277, 3444
BfrI CTTAAG 1 cut(s) 2021
BfuI GTATCC 1 cut(s) 1031
BglII AGATCT 2 cut(s) 559, 3134
BlpI GCTNAGC 2 cut(s) 1476, 2358
Bme1390I CCNGG 6 cut(s) 267, 408, 409, 594, 962, 1470
Bme18I GGWCC 5 cut(s) 263, 1834, 2468, 2797, 3928
BmeT110I CYCGRG 2 cut(s) 407, 2974
BmgT120I GGNCC 6 cut(s) 263, 1834, 2468, 2652, 2797, 3928
BmiI GGNNCC 6 cut(s) 264, 350, 1901, 2343, 2406, 2799
BmrFI CCNGG 6 cut(s) 267, 408, 409, 594, 962, 1470
BpiI GAAGAC 7 cut(s) 2657, 2858, 3065, 3265, 3567, 3591, 3787
BplI GAGNNNNNCTC 3 cut(s) 37, 3452, 3484
BpmI CTGGAG 3 cut(s) 1695, 1980, 2061
Bpu10I CCTNAGC 4 cut(s) 209, 2265, 2409, 2580
Bpu1102I GCTNAGC 2 cut(s) 1476, 2358
Bpu14I TTCGAA 2 cut(s) 1550, 2951
BpuEI CTTGAG 4 cut(s) 204, 1383, 2625, 4127
BpuMI CCSGG 2 cut(s) 408, 409
Bsa29I ATCGAT 1 cut(s) 172
BsaAI YACGTR 2 cut(s) 417, 2996
BsaHI GRCGYC 1 cut(s) 1751
BsaJI CCNNGG 5 cut(s) 407, 593, 628, 687, 2320
BsaWI WCCGGW 2 cut(s) 1867, 2331
BsaXI ACNNNNNCTCC 2 cut(s) 388, 418
Bse118I RCCGGY 1 cut(s) 40
Bse1I ACTGG 6 cut(s) 403, 443, 1024, 2358, 3310, 3319
Bse21I CCTNAGG 2 cut(s) 697, 3627
Bse3DI GCAATG 3 cut(s) 2264, 3235, 3522
BseBI CCWGG 4 cut(s) 267, 594, 962, 1470
BseCI ATCGAT 1 cut(s) 172
BseDI CCNNGG 5 cut(s) 407, 593, 628, 687, 2320
BseMI GCAATG 3 cut(s) 2264, 3235, 3522
BseMII CTCAG 9 cut(s) 1343, 1359, 1490, 1782, 2256, 2630, 3014, 3618, 3686
BseNI ACTGG 6 cut(s) 403, 443, 1024, 2358, 3310, 3319
BseRI GAGGAG 2 cut(s) 2282, 2453
BseSI GKGCMC 1 cut(s) 3326
BseX3I CGGCCG 1 cut(s) 42
BsgI GTGCAG 2 cut(s) 706, 1632
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 5 cut(s) 44, 960, 2032, 2212, 2654
BshNI GGYRCC 1 cut(s) 1899
BshVI ATCGAT 1 cut(s) 172
BsiEI CGRYCG 1 cut(s) 45
BsiHKAI GWGCWC 4 cut(s) 1477, 2556, 3001, 3976
BsiHKCI CYCGRG 2 cut(s) 407, 2974
BsiSI CCGG 5 cut(s) 41, 408, 1868, 2254, 2332
BslFI GGGAC 3 cut(s) 2223, 2710, 2783
BsmAI GTCTC 5 cut(s) 458, 1713, 1931, 3443, 3637
BsmFI GGGAC 3 cut(s) 2223, 2710, 2783
BsmI GAATGC 1 cut(s) 2716
BsnI GGCC 5 cut(s) 44, 960, 2032, 2212, 2654
BsoBI CYCGRG 2 cut(s) 407, 2974
Bsp119I TTCGAA 2 cut(s) 1550, 2951
Bsp1286I GDGCHC 5 cut(s) 1477, 2556, 3001, 3326, 3976
Bsp1720I GCTNAGC 2 cut(s) 1476, 2358
Bsp19I CCATGG 1 cut(s) 2320
BspACI CCGC 6 cut(s) 11, 45, 213, 239, 2869, 3236
BspANI GGCC 5 cut(s) 44, 960, 2032, 2212, 2654
BspCNI CTCAG 9 cut(s) 1342, 1358, 1489, 1781, 2257, 2629, 3013, 3619, 3685
BspDI ATCGAT 1 cut(s) 172
BspHI TCATGA 2 cut(s) 1365, 3858
BspLI GGNNCC 6 cut(s) 264, 350, 1901, 2343, 2406, 2799
BspMAI CTGCAG 2 cut(s) 1603, 2277
BspPI GGATC 6 cut(s) 223, 2336, 2349, 3227, 3471, 4146
BspQI GCTCTTC 1 cut(s) 1669
BspT104I TTCGAA 2 cut(s) 1550, 2951
BspT107I GGYRCC 1 cut(s) 1899
BspTI CTTAAG 1 cut(s) 2021
BsrDI GCAATG 3 cut(s) 2264, 3235, 3522
BsrFI RCCGGY 1 cut(s) 40
BsrI ACTGG 6 cut(s) 403, 443, 1024, 2358, 3310, 3319
BssAI RCCGGY 1 cut(s) 40
BssECI CCNNGG 5 cut(s) 407, 593, 628, 687, 2320
BssNAI GTATAC 1 cut(s) 2770
BssNI GRCGYC 1 cut(s) 1751
BssSI CACGAG 4 cut(s) 139, 1797, 2242, 3165
BssT1I CCWWGG 2 cut(s) 628, 2320
Bst1107I GTATAC 1 cut(s) 2770
Bst2BI CACGAG 4 cut(s) 139, 1797, 2242, 3165
Bst2UI CCWGG 4 cut(s) 267, 594, 962, 1470
Bst6I CTCTTC 5 cut(s) 1669, 3102, 3484, 3592, 4061
BstACI GRCGYC 1 cut(s) 1751
BstAFI CTTAAG 1 cut(s) 2021
BstAPI GCANNNNNTGC 2 cut(s) 3225, 3336
BstBAI YACGTR 2 cut(s) 417, 2996
BstBI TTCGAA 2 cut(s) 1550, 2951
BstC8I GCNNGC 3 cut(s) 1541, 2214, 2569
BstDSI CCRYGG 2 cut(s) 687, 2320
BstENI CCTNNNNNAGG 1 cut(s) 3845
BstHHI GCGC 1 cut(s) 3515
BstMAI GTCTC 5 cut(s) 458, 1713, 1931, 3443, 3637
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 6 cut(s) 2585, 2877, 3209, 3225, 3336, 3739
BstNI CCWGG 4 cut(s) 267, 594, 962, 1470
BstNSI RCATGY 5 cut(s) 536, 1150, 1380, 1543, 2138
BstSCI CCNGG 6 cut(s) 265, 406, 407, 592, 960, 1468
BstSFI CTRYAG 5 cut(s) 1599, 2273, 2925, 3277, 3444
BstSLI GKGCMC 1 cut(s) 3326
BstSNI TACGTA 1 cut(s) 417
BstV2I GAAGAC 7 cut(s) 2657, 2858, 3065, 3265, 3567, 3591, 3787
BstX2I RGATCY 5 cut(s) 215, 559, 2341, 3134, 4138
BstXI CCANNNNNNTGG 1 cut(s) 273
BstYI RGATCY 5 cut(s) 215, 559, 2341, 3134, 4138
BstZ17I GTATAC 1 cut(s) 2770
BstZI CGGCCG 1 cut(s) 42
Bsu15I ATCGAT 1 cut(s) 172
Bsu36I CCTNAGG 2 cut(s) 697, 3627
BsuI GTATCC 1 cut(s) 1031
BsuRI GGCC 5 cut(s) 44, 960, 2032, 2212, 2654
BsuTUI ATCGAT 1 cut(s) 172
BtgI CCRYGG 2 cut(s) 687, 2320
BtgZI GCGATG 1 cut(s) 3324
Cac8I GCNNGC 3 cut(s) 1541, 2214, 2569
CaiI CAGNNNCTG 3 cut(s) 2219, 3023, 3225
CciI TCATGA 2 cut(s) 1365, 3858
CfoI GCGC 1 cut(s) 3515
Cfr10I RCCGGY 1 cut(s) 40
Cfr13I GGNCC 6 cut(s) 263, 1834, 2468, 2652, 2797, 3928
Cfr9I CCCGGG 1 cut(s) 407
ClaI ATCGAT 1 cut(s) 172
CseI GACGC 3 cut(s) 1211, 1759, 3059
Csp6I GTAC 6 cut(s) 418, 2461, 2827, 3472, 3775, 4147
CviQI GTAC 6 cut(s) 418, 2461, 2827, 3472, 3775, 4147
DraI TTTAAA 2 cut(s) 954, 1014
DrdI GACNNNNNNGTC 1 cut(s) 1147
DseDI GACNNNNNNGTC 1 cut(s) 1147
EaeI YGGCCR 2 cut(s) 42, 958
EagI CGGCCG 1 cut(s) 42
Eam1104I CTCTTC 5 cut(s) 1669, 3102, 3484, 3592, 4061
EarI CTCTTC 5 cut(s) 1669, 3102, 3484, 3592, 4061
EciI GGCGGA 2 cut(s) 26, 3225
Ecl136II GAGCTC 1 cut(s) 3974
EclXI CGGCCG 1 cut(s) 42
Eco105I TACGTA 1 cut(s) 417
Eco130I CCWWGG 2 cut(s) 628, 2320
Eco147I AGGCCT 1 cut(s) 2032
Eco24I GRGCYC 1 cut(s) 3976
Eco47I GGWCC 5 cut(s) 263, 1834, 2468, 2797, 3928
Eco52I CGGCCG 1 cut(s) 42
Eco53kI GAGCTC 1 cut(s) 3974
Eco72I CACGTG 1 cut(s) 2996
Eco81I CCTNAGG 2 cut(s) 697, 3627
Eco88I CYCGRG 2 cut(s) 407, 2974
EcoICRI GAGCTC 1 cut(s) 3974
EcoNI CCTNNNNNAGG 1 cut(s) 3845
EcoO109I RGGNCCY 3 cut(s) 1834, 2652, 2797
EcoRI GAATTC 4 cut(s) 68, 1450, 1965, 3676
EcoRII CCWGG 4 cut(s) 265, 592, 960, 1468
EcoT14I CCWWGG 2 cut(s) 628, 2320
EcoT22I ATGCAT 3 cut(s) 840, 1545, 3290
EcoT38I GRGCYC 1 cut(s) 3976
ErhI CCWWGG 2 cut(s) 628, 2320
FalI AAGNNNNNCTT 6 cut(s) 2329, 2361, 2868, 2900, 3738, 3770
FaqI GGGAC 3 cut(s) 2223, 2710, 2783
FbaI TGATCA 3 cut(s) 1279, 1978, 3088
FblI GTMKAC 3 cut(s) 105, 1258, 2769
FriOI GRGCYC 1 cut(s) 3976
FspI TGCGCA 1 cut(s) 3514
GlaI GCGC 1 cut(s) 3514
GsuI CTGGAG 3 cut(s) 1695, 1980, 2061
HaeIII GGCC 5 cut(s) 44, 960, 2032, 2212, 2654
HapII CCGG 5 cut(s) 41, 408, 1868, 2254, 2332
HgaI GACGC 3 cut(s) 1211, 1759, 3059
HhaI GCGC 1 cut(s) 3515
Hin1I GRCGYC 1 cut(s) 1751
Hin6I GCGC 1 cut(s) 3513
HinP1I GCGC 1 cut(s) 3513
HincII GTYRAC 2 cut(s) 106, 3049
HindII GTYRAC 2 cut(s) 106, 3049
HindIII AAGCTT 3 cut(s) 297, 3081, 3201
HpaI GTTAAC 1 cut(s) 3049
HpaII CCGG 5 cut(s) 41, 408, 1868, 2254, 2332
HphI GGTGA 3 cut(s) 3083, 3673, 3862
Hpy166II GTNNAC 9 cut(s) 106, 1259, 2317, 2471, 2770, 3049, 3524, 3723, 4115
Hpy8I GTNNAC 9 cut(s) 106, 1259, 2317, 2471, 2770, 3049, 3524, 3723, 4115
Hpy99I CGWCG 3 cut(s) 77, 89, 2435
HpyCH4IV ACGT 5 cut(s) 416, 2677, 2995, 3504, 3633
HpyF10VI GCNNNNNNNGC 6 cut(s) 2585, 2877, 3209, 3225, 3336, 3739
HpySE526I ACGT 5 cut(s) 416, 2677, 2995, 3504, 3633
Hsp92I GRCGYC 1 cut(s) 1751
HspAI GCGC 1 cut(s) 3513
Ksp22I TGATCA 3 cut(s) 1279, 1978, 3088
KspAI GTTAAC 1 cut(s) 3049
LguI GCTCTTC 1 cut(s) 1669
LmnI GCTCC 4 cut(s) 801, 1699, 2042, 2269
MaeII ACGT 5 cut(s) 416, 2677, 2995, 3504, 3633
MfeI CAATTG 6 cut(s) 1745, 1808, 2007, 2388, 2715, 3963
MflI RGATCY 5 cut(s) 215, 559, 2341, 3134, 4138
MhlI GDGCHC 5 cut(s) 1477, 2556, 3001, 3326, 3976
MlsI TGGCCA 1 cut(s) 960
MluNI TGGCCA 1 cut(s) 960
MlyI GAGTC 7 cut(s) 1082, 1292, 2692, 3342, 3777, 3985, 4114
MmeI TCCRAC 2 cut(s) 941, 2840
Mox20I TGGCCA 1 cut(s) 960
Mph1103I ATGCAT 3 cut(s) 840, 1545, 3290
MroXI GAANNNNTTC 3 cut(s) 1072, 1569, 1860
MscI TGGCCA 1 cut(s) 960
MslI CAYNNNNRTG 5 cut(s) 499, 728, 1918, 2139, 3863
Msp20I TGGCCA 1 cut(s) 960
MspA1I CMGCKG 2 cut(s) 2216, 3020
MspCI CTTAAG 1 cut(s) 2021
MspI CCGG 5 cut(s) 41, 408, 1868, 2254, 2332
MspR9I CCNGG 6 cut(s) 267, 408, 409, 594, 962, 1470
MunI CAATTG 6 cut(s) 1745, 1808, 2007, 2388, 2715, 3963
Mva1269I GAATGC 1 cut(s) 2716
MvaI CCWGG 4 cut(s) 267, 594, 962, 1470
MwoI GCNNNNNNNGC 6 cut(s) 2585, 2877, 3209, 3225, 3336, 3739
NciI CCSGG 2 cut(s) 408, 409
NcoI CCATGG 1 cut(s) 2320
NlaIV GGNNCC 6 cut(s) 264, 350, 1901, 2343, 2406, 2799
NmeAIII GCCGAG 1 cut(s) 1779
NmuCI GTSAC 4 cut(s) 390, 1391, 3661, 3850
NsbI TGCGCA 1 cut(s) 3514
NsiI ATGCAT 3 cut(s) 840, 1545, 3290
NspI RCATGY 5 cut(s) 536, 1150, 1380, 1543, 2138
NspV TTCGAA 2 cut(s) 1550, 2951
PaeI GCATGC 1 cut(s) 1543
PaeR7I CTCGAG 1 cut(s) 2974
PagI TCATGA 2 cut(s) 1365, 3858
PceI AGGCCT 1 cut(s) 2032
PciI ACATGT 1 cut(s) 1146
PciSI GCTCTTC 1 cut(s) 1669
PcsI WCGNNNNNNNCGW 3 cut(s) 81, 1695, 2674
PctI GAATGC 1 cut(s) 2716
PdmI GAANNNNTTC 3 cut(s) 1072, 1569, 1860
PflMI CCANNNNNTGG 3 cut(s) 1718, 1871, 2219
PleI GAGTC 7 cut(s) 1082, 1292, 2692, 3342, 3777, 3985, 4114
PmaCI CACGTG 1 cut(s) 2996
PmlI CACGTG 1 cut(s) 2996
PpsI GAGTC 7 cut(s) 1082, 1292, 2692, 3342, 3777, 3985, 4114
Ppu21I YACGTR 2 cut(s) 417, 2996
PpuMI RGGWCCY 2 cut(s) 1834, 2797
PscI ACATGT 1 cut(s) 1146
PshBI ATTAAT 1 cut(s) 3291
Psp124BI GAGCTC 1 cut(s) 3976
Psp5II RGGWCCY 2 cut(s) 1834, 2797
Psp6I CCWGG 4 cut(s) 265, 592, 960, 1468
PspCI CACGTG 1 cut(s) 2996
PspGI CCWGG 4 cut(s) 265, 592, 960, 1468
PspN4I GGNNCC 6 cut(s) 264, 350, 1901, 2343, 2406, 2799
PspPI GGNCC 6 cut(s) 263, 1834, 2468, 2652, 2797, 3928
PspPPI RGGWCCY 2 cut(s) 1834, 2797
PspXI VCTCGAGB 1 cut(s) 2974
PstI CTGCAG 2 cut(s) 1603, 2277
PstNI CAGNNNCTG 3 cut(s) 2219, 3023, 3225
PsuI RGATCY 5 cut(s) 215, 559, 2341, 3134, 4138
PvuII CAGCTG 2 cut(s) 2216, 3020
RsaI GTAC 6 cut(s) 419, 2462, 2828, 3473, 3776, 4148
RsaNI GTAC 6 cut(s) 418, 2461, 2827, 3472, 3775, 4147
RseI CAYNNNNRTG 5 cut(s) 499, 728, 1918, 2139, 3863
SacI GAGCTC 1 cut(s) 3976
SalI GTCGAC 1 cut(s) 104
SapI GCTCTTC 1 cut(s) 1669
Sau96I GGNCC 6 cut(s) 263, 1834, 2468, 2652, 2797, 3928
SchI GAGTC 7 cut(s) 1082, 1292, 2692, 3342, 3777, 3985, 4114
ScrFI CCNGG 6 cut(s) 267, 408, 409, 594, 962, 1470
SduI GDGCHC 5 cut(s) 1477, 2556, 3001, 3326, 3976
SfcI CTRYAG 5 cut(s) 1599, 2273, 2925, 3277, 3444
Sfr274I CTCGAG 1 cut(s) 2974
SfuI TTCGAA 2 cut(s) 1550, 2951
SinI GGWCC 5 cut(s) 263, 1834, 2468, 2797, 3928
SlaI CTCGAG 1 cut(s) 2974
SmaI CCCGGG 1 cut(s) 409
SmiI ATTTAAAT 1 cut(s) 954
SmiMI CAYNNNNRTG 5 cut(s) 499, 728, 1918, 2139, 3863
SmlI CTYRAG 6 cut(s) 219, 1398, 2021, 2640, 2974, 4142
SmoI CTYRAG 6 cut(s) 219, 1398, 2021, 2640, 2974, 4142
SnaBI TACGTA 1 cut(s) 417
SpeI ACTAGT 1 cut(s) 1682
SphI GCATGC 1 cut(s) 1543
SseBI AGGCCT 1 cut(s) 2032
SsiI CCGC 6 cut(s) 11, 45, 213, 239, 2869, 3236
SstI GAGCTC 1 cut(s) 3976
StuI AGGCCT 1 cut(s) 2032
StyD4I CCNGG 6 cut(s) 265, 406, 407, 592, 960, 1468
StyI CCWWGG 2 cut(s) 628, 2320
SwaI ATTTAAAT 1 cut(s) 954
TaiI ACGT 5 cut(s) 419, 2680, 2998, 3507, 3636
TatI WGTACW 1 cut(s) 3774
TauI GCSGC 2 cut(s) 47, 2871
TseFI GTSAC 4 cut(s) 390, 1391, 3661, 3850
Tsp45I GTSAC 4 cut(s) 390, 1391, 3661, 3850
TspGWI ACGGA 2 cut(s) 704, 2907
TspMI CCCGGG 1 cut(s) 407
Van91I CCANNNNNTGG 3 cut(s) 1718, 1871, 2219
Vha464I CTTAAG 1 cut(s) 2021
VpaK11BI GGWCC 5 cut(s) 263, 1834, 2468, 2797, 3928
VspI ATTAAT 1 cut(s) 3291
XagI CCTNNNNNAGG 1 cut(s) 3845
XbaI TCTAGA 1 cut(s) 656
XceI RCATGY 5 cut(s) 536, 1150, 1380, 1543, 2138
XcmI CCANNNNNNNNNTGG 1 cut(s) 1031
XhoI CTCGAG 1 cut(s) 2974
XmaI CCCGGG 1 cut(s) 407
XmiI GTMKAC 3 cut(s) 105, 1258, 2769
XmnI GAANNNNTTC 3 cut(s) 1072, 1569, 1860
Zsp2I ATGCAT 3 cut(s) 840, 1545, 3290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.