RLG00000031464

SNF2 family N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0004421
Physical Location & Seq
Forward (+)
5419709 .. 5427826
8118 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031464

Sequence Viewer

Length: 4365 bp
ATGTTGATGGCGGTGGAGGAGAGTTCTAATTGTGGATTCGCGGCCGAGGATTTCGACGGCGAGGACATGTCGATCGATCTCGATACATTTTACCGCCTTCTCGAGGAAGAACCGCCGAGTTGTCCGGAGGATTCATCATTGGGAAGCGGATCACAAGCTGAAGCAGTTTCCGGTGCTGATTACCGCAAGGATACACAGCTCCAAAGTGGATCGCAAGTTTTAAAAGCAGAACCATCAGCTGATTTAGGGGGTTTCGGATCATGGTATCCTCCATACAGCTCTGAAGCTTCGGATTCTGGGGCAGGGGGTCCAGGTGGTTCCTTTGATTCTGCAGGGAACCCTGCAATGTTCTCTGACTACAAAGATAGCAAACCAACAGCATACACTGGCTCTCCTGGTCCATGGCATCCTCCATATATCTCAGAAGCATCAGATTCTGTGGCAGGGGGTCCAGGTGGTTCCTTTGAGTGTGGAGGGAATCCTGCAATGTCTTTTGACAGGAAACCACCAGCACAGACCAGCTCTCATGGGCACATGTTCTCTACAAGTCTTAAAGACTGGATTTCGTTGGTTCCGTGCACGGAGACTTACTTGACTGAGAGGGTTGACGTTTCACAGCCCACTAGTAGCACCACTTCTAGTTTTGAAGAAGGACATACTAATCATGTTTTAGACCACGGAGATCTTAATGTCCTGCAGGGAAAGGCAGATGTGGCAGGGAAAGTTGATTCTGAATATTCTTCACAAAACCTTGGTATTGATAACATGGACATGAATTCCAGACCGTATGGTGCTTTGGGAGAAAACACCCCAGAAACCTTGGGACCATCAGAAAATAATTCTTGTACATCTATGGAAATACCCTTTCTAGATGTAGATATATGTTCACCACATGTTACTTCTACCGAATCTACTCTCTGTCAAAATTCTGATCTTTTCGGTGACCATTATACAGCTGTGGATGGTATGAGCTTAGACAATAGATTTTTGGCAGAGTCCTCAATCCAGCATAGTCCTTATAGCTACTACTCTACATTATTTCCAAGTAACAAAGAAATGATGACTAATGTGAAGGATGAATCACTGGAGTTTCAAACTGATAGTTCATGCTCAAGCAGTAAGATGAATATCAATTGTCAGGAGGGAATGATCGGTGCATATGGCTTTGATTCGCCAATGATTGATGCTTCAGATATCAAGGAGTGGAATTTTGATTATGGAAGATATAATTGTATTTCGGCTGTTAGTGGGAATTCTTCATTGGATGCTGACTCTTGTCCTGTTGACAACAAGGCTTCAGTCAAGCCTTTGGGTTCTACTGAGACATACATGTCAAGCAAACGGGAATTCACTGGTGTGAAAGATGAAAATATTGATGAGTTGTTTGCTCCTAGTACTGATACGTTTCATTCTTTTTCATTCATGGATGACAGTAGAAAGCCATCTTACAATGCTGATGGTAGCTTTTTCGACAAGGAACCAAAGCTCTCAGGTTATGATGTTTCTACTCAGCCTTTTGGAAATTCAGGGCATGCTAAAGAAGAGATGATTGTTGATACTAAGAGAACTTATTATTCTGAAGGTAATATAAATGGAAGTTCTTTACCCCATGTTGGTTGTGCATCTGTGAATCTAAACGGTTTGGAACATCAGTTGCCAGCTGCTCAGCCATTCTCTTCAAACAGGAATCAGGTTTATAGTTCGGACAGACTTGAGGGTAAACACTGTCTAACTAAGAGCATGGGTTTTCCTTCTTCAAAAGTCAGCCCCGAGTCTATTCATAGTAACTTCTCTGAAAAATCCCCTGCAGAAGATGACTATGATATATGCATTATTGAAGATAAGAGTGATCCTGCACCCACATGTCGCCTTCCAGTGGTTAGCAATACTCGTTACCCGGCGCCCTTGAATCGCTCTCTAGCAGTTGGAAGTAACATTGTTAGTTCACAACAATCTAGTGATCACGACACAGGAGTGGGAGGCATACGATTCAGGACAAGGGATGAGCAATTGATCTTGAGAGTAGCATTGCAGGACCTTTCACAGCCCAAGTCAGAAGCTCTTCCACCAGATGGAGTTTTAACAGTACCTCTCTTACGACATCAGAGAATTGCATTGTCATGGATGGTTCAGAAGGAGACAGCTAGCTTGCATTGTTGCGGAGGAATACTTGCAGATGATCAGGGATTGGGAAAAACGATATCGACAATTGCACTCATACTTAAGGAAAGGCCTCCTGCTTCTGGAGCCTGTCTAGATGAAAAAAAGTGCAAGTTGGAAACTCTGGATTTGGACCAGGATGATGACATGCTTCCTGAGGTTAGCAGAAGGAAGCAAGATGCTGCTGCTCATTCAAGTGTCTCAAATGAAACTTCTGAGAAGAGCATGAAATCTTTGATACAAACAAAGGGAAGGCCAGCTTGTGGAACACTCGTTGTTTGTCCCACCAGTGTCTTGCGGCAATGGGCAGAGGAGTTGCGTAATAAAATAACAGAAAAAGCTAAACTCTCAGTGCTAGTCTACCATGGAGGCAATCGAACGAGGGATCCGTGTGAGCTAGCTAAGTATGATGTTGTCCTCACAACATATTCAATTGTCAGCATGGAGGTCCCAAAGCAGCCTCTTGCTGATGGAGAAGATGAGGAGAAAGGAAAGCGAGAGGAGTATGATTCTCCACATATGGGGTTTTCATCTAAGAAAAGGAAATATCCTAATAAATGCTCAAAGGGTAAGAAGGGATTGGAGACTGCAGCGCTTGAGTCTCTTGCTCGCCCTCTTGCTAAGGTTGGATGGTTTAGGGTTGTCTTGGATGAAGCCCAGAGTATTAAGAATCACAGAACTCAAGTAGCTAGGGCCTGTTGGGGGCTTCGAGCGAAACGTAGATGGTGCTTATCGGGGACACCTATCCAGAATGCAATTGATGATCTTTATAGCTACTTCAGATTTCTGAGATATGACCCTTATGCGGTGTACAAGTCGTTTTGTGCAAAAATAAAATTTCCAATTAGTAAGAATCCAACAAAAGGGTACAGAATGCTACAAGCTGTTCTGAAGACAATTATGTTACGCCGCACAAAAGGTACTCTTCTTGATGGACAACCTATTATTAATTTACCACCAAAATTTATAGAACTGAAAAGGGTGGAATTCTCCACGGAAGAACGAGATTTTTACTCAAGGCTAGAGAGTGATTCACGCGCTCAATTTGAAGAATATGCAGCTGCTGGAACTGTGAAACAAAATTATGTTAATATTTTATTGATGCTCTTGCGACTTCGACAAGCTTGTGATCACCCTCTTCTTGTCAGACGTTATGAATCACAGTCTTTGTGGAAATCTTCGGTTGAGAAGGCAAAGAAGCTTACTCATGACAAACAAGTATCCCTTCTGAATTGTTTGGAAGCTTCTTTGGCAATATGCGGTATCTGCAATGATGCACCTGAAGATGCCGTTGTTTCTGAATGTGGTCATGTGTTCTGTAGTCAATGCATTTGTGACTATCTCACTGGTGATGACAACCAGTGCCCCAACACAAGTTGCAAAGTTCGACTGAATGTGTCTTCCGTCTTTTCAAAAGCCACTTTAAACAGTTCGCTTTCTGACCAACCTAGTGAAGGGGGCATGGGTTCTGAAGTTTTTGATGCTGTAGAGTCTTTCTATGAGGATAGCTCGTACAATTCATCCAAGATTAAGGCTGCACTTGAGGTTCTCTGCGTGATGTGTAAACCAAAGACTTGCACTACAGAAAATAGGTGCTTACCGGAAAGCGTTGATAATAATGCCAGCTGTTCTACAACGTCTTTTGACATTGATGGGGCTGAGTCCCTTGAGGACGGTTCTGATGGACAGAACTTGGGTGTGGACAAAAGTCCTAAGAAAATTGAGAAGGTAGTTCGAGAAAAAGCAATAGTCTTTTCACAGTGGACTAGGATGCTGGACTTGCTTGAAGCGTGTCTTAAAACTTCCGGCCTTGAGTACAGAAGACTTGATGGAACGATGTCAGTTGTTGCCAGAGATAAAGCTGTAAAGGATTTTAACACTCTTCCTGAGGTGTCAGTTATGATTATGTCTTTGAAAGCTGCTAGTCTTGGTCTGAACATGGTTGCAGCCTGCCATGTACTTCTTTTGGACCTGTGGTGGAATCCTACGACTGAAGATCAAGCAATTGATAGAGCACATCGTATTGGGCAGACTCGTCCTGTTACGGTTTTACGGTTGACTGTGAGAGATACGGTCGAAGATCGTATTTTAGCTCTTCAGAAAAAGAAGAGAGAGATGGTGGCATCTGCATTTGGAGAGGATGAGACTGGTGGTCGTCAGACTCGCTTAACAGTTGATGATCTCAAGTACCTGTTTATGAAGTGA

Protein Analysis

1455

Amino Acids

159.89

Weight (kDa)

5.16

Isoelectric Point (pI)

52.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNF2-rel_dom PF00176 701 - 1099 5.2e-65 SNF2-related domain
zf-C3HC4 PF00097 1140 - 1176 4e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 1140 - 1176 1.2e-07 Zinc finger, C3HC4 type (RING finger)
Helicase_C PF00271 1296 - 1396 9.5e-20 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1330
AccB1I GGYRCC 1 cut(s) 1900
AccB7I CCANNNNNTGG 2 cut(s) 2072, 2423
AccI GTMKAC 1 cut(s) 2520
AccII CGCG 2 cut(s) 41, 3198
AccIII TCCGGA 1 cut(s) 124
AclWI GGATC 6 cut(s) 157, 217, 265, 1844, 2540, 2553
AcoI YGGCCR 1 cut(s) 42
AcsI RAATTY 9 cut(s) 775, 925, 1207, 1252, 1346, 1522, 2996, 3122, 3146
AcyI GRCGYC 1 cut(s) 1901
AfeI AGCGCT 1 cut(s) 2754
AflII CTTAAG 1 cut(s) 2222
AflIII ACRYGT 5 cut(s) 66, 534, 892, 1329, 1862
AhdI GACNNNNNGTC 1 cut(s) 4311
AhlI ACTAGT 1 cut(s) 623
AjnI CCWGG 4 cut(s) 310, 394, 451, 2295
AjuI GAANNNNNNNTTGG 2 cut(s) 1244, 1276
AleI CACNNNNGTG 2 cut(s) 1355, 1973
AloI GAACNNNNNNTCC 2 cut(s) 2530, 2562
Alw21I GWGCWC 2 cut(s) 581, 4177
Alw26I GTCTC 7 cut(s) 578, 1316, 2132, 2365, 2738, 2766, 4298
Alw44I GTGCAC 1 cut(s) 577
AlwI GGATC 6 cut(s) 157, 217, 265, 1844, 2540, 2553
AlwNI CAGNNNCTG 2 cut(s) 437, 3224
Ama87I CYCGRG 2 cut(s) 101, 1769
Aor13HI TCCGGA 1 cut(s) 124
Aor51HI AGCGCT 1 cut(s) 2754
AoxI GGCC 5 cut(s) 42, 2231, 2414, 2853, 3967
ApaLI GTGCAC 1 cut(s) 577
ApoI RAATTY 9 cut(s) 775, 925, 1207, 1252, 1346, 1522, 2996, 3122, 3146
AseI ATTAAT 1 cut(s) 3108
Asp700I GAANNNNTTC 1 cut(s) 2061
AspLEI GCGC 3 cut(s) 1903, 2755, 3200
AspS9I GGNCC 9 cut(s) 308, 398, 449, 824, 2035, 2293, 2608, 2853, 4129
AsuC2I CCSGG 1 cut(s) 1898
AsuHPI GGTGA 4 cut(s) 879, 953, 3284, 3521
AsuNHI GCTAGC 2 cut(s) 2144, 2557
AvaI CYCGRG 2 cut(s) 101, 1769
AvaII GGWCC 8 cut(s) 308, 398, 449, 824, 2035, 2293, 2608, 4129
AxyI CCTNAGG 2 cut(s) 2316, 4047
BaeGI GKGCMC 3 cut(s) 534, 581, 3527
BamHI GGATCC 1 cut(s) 2545
BanI GGYRCC 1 cut(s) 1900
BarI GAAGNNNNNNTAC 2 cut(s) 619, 651
BbsI GAAGAC 3 cut(s) 3059, 3552, 3988
Bbv12I GWGCWC 2 cut(s) 581, 4177
BceAI ACGGC 2 cut(s) 73, 3434
BciT130I CCWGG 4 cut(s) 312, 396, 453, 2297
BciVI GTATCC 3 cut(s) 184, 276, 3391
BclI TGATCA 3 cut(s) 1960, 2179, 3289
BcnI CCSGG 1 cut(s) 1898
BcoDI GTCTC 7 cut(s) 578, 1316, 2132, 2365, 2738, 2766, 4298
BcuI ACTAGT 1 cut(s) 623
BfmI CTRYAG 7 cut(s) 330, 695, 1806, 2748, 3478, 3645, 3741
BfoI RGCGCY 2 cut(s) 1904, 2756
BfrI CTTAAG 1 cut(s) 2222
BfuI GTATCC 3 cut(s) 184, 276, 3391
BglII AGATCT 1 cut(s) 682
BlpI GCTNAGC 1 cut(s) 1665
BmcAI AGTACT 1 cut(s) 1396
Bme1390I CCNGG 5 cut(s) 312, 396, 453, 1898, 2297
Bme18I GGWCC 8 cut(s) 308, 398, 449, 824, 2035, 2293, 2608, 4129
BmeRI GACNNNNNGTC 1 cut(s) 4311
BmeT110I CYCGRG 2 cut(s) 101, 1769
BmgT120I GGNCC 9 cut(s) 308, 398, 449, 824, 2035, 2293, 2608, 2853, 4129
BmrFI CCNGG 5 cut(s) 312, 396, 453, 1898, 2297
BmtI GCTAGC 2 cut(s) 2148, 2561
BoxI GACNNNNGTC 2 cut(s) 1275, 3867
BpiI GAAGAC 3 cut(s) 3059, 3552, 3988
BplI GAGNNNNNCTC 2 cut(s) 3653, 3685
BpmI CTGGAG 2 cut(s) 1106, 2265
Bpu10I CCTNAGC 1 cut(s) 2781
Bpu1102I GCTNAGC 1 cut(s) 1665
BpuMI CCSGG 1 cut(s) 1898
Bsa29I ATCGAT 1 cut(s) 75
BsaBI GATNNNNATC 1 cut(s) 1127
BsaHI GRCGYC 1 cut(s) 1901
BsaJI CCNNGG 7 cut(s) 45, 401, 676, 751, 819, 2524, 3153
BsaWI WCCGGW 3 cut(s) 124, 170, 3760
BsaXI ACNNNNNCTCC 2 cut(s) 376, 406
Bse1I ACTGG 9 cut(s) 391, 563, 1089, 1357, 1874, 2448, 3511, 3520, 4312
Bse21I CCTNAGG 2 cut(s) 2316, 4047
Bse3DI GCAATG 5 cut(s) 351, 492, 2027, 2468, 3436
Bse8I GATNNNNATC 1 cut(s) 1127
BseAI TCCGGA 1 cut(s) 124
BseBI CCWGG 4 cut(s) 312, 396, 453, 2297
BseCI ATCGAT 1 cut(s) 75
BseDI CCNNGG 7 cut(s) 45, 401, 676, 751, 819, 2524, 3153
BseJI GATNNNNATC 1 cut(s) 1127
BseMI GCAATG 5 cut(s) 351, 492, 2027, 2468, 3436
BseNI ACTGG 9 cut(s) 391, 563, 1089, 1357, 1874, 2448, 3511, 3520, 4312
BseRI GAGGAG 4 cut(s) 32, 2486, 2657, 2675
BseSI GKGCMC 3 cut(s) 534, 581, 3527
BseX3I CGGCCG 1 cut(s) 42
BsgI GTGCAG 2 cut(s) 1839, 3681
Bsh1236I CGCG 2 cut(s) 41, 3198
Bsh1285I CGRYCG 3 cut(s) 45, 75, 4236
BshFI GGCC 5 cut(s) 44, 2233, 2416, 2855, 3969
BshNI GGYRCC 1 cut(s) 1900
BshVI ATCGAT 1 cut(s) 75
BsiEI CGRYCG 3 cut(s) 45, 75, 4236
BsiHKAI GWGCWC 2 cut(s) 581, 4177
BsiHKCI CYCGRG 2 cut(s) 101, 1769
BsiSI CCGG 5 cut(s) 125, 171, 1898, 3761, 3966
BslFI GGGAC 5 cut(s) 837, 2427, 2594, 2911, 3808
BsmAI GTCTC 7 cut(s) 578, 1316, 2132, 2365, 2738, 2766, 4298
BsmFI GGGAC 5 cut(s) 837, 2427, 2594, 2911, 3808
BsmI GAATGC 2 cut(s) 2917, 3039
BsnI GGCC 5 cut(s) 44, 2233, 2416, 2855, 3969
BsoBI CYCGRG 2 cut(s) 101, 1769
Bsp1286I GDGCHC 4 cut(s) 534, 581, 3527, 4177
Bsp13I TCCGGA 1 cut(s) 124
Bsp1407I TGTACA 2 cut(s) 845, 2970
Bsp1720I GCTNAGC 1 cut(s) 1665
Bsp19I CCATGG 2 cut(s) 401, 2524
BspANI GGCC 5 cut(s) 44, 2233, 2416, 2855, 3969
BspDI ATCGAT 1 cut(s) 75
BspEI TCCGGA 1 cut(s) 124
BspFNI CGCG 2 cut(s) 41, 3198
BspHI TCATGA 1 cut(s) 3367
BspMAI CTGCAG 4 cut(s) 334, 699, 1810, 2752
BspOI GCTAGC 2 cut(s) 2148, 2561
BspPI GGATC 6 cut(s) 157, 217, 265, 1844, 2540, 2553
BspQI GCTCTTC 3 cut(s) 2067, 2375, 4260
BspT107I GGYRCC 1 cut(s) 1900
BspTI CTTAAG 1 cut(s) 2222
BsrDI GCAATG 5 cut(s) 351, 492, 2027, 2468, 3436
BsrGI TGTACA 2 cut(s) 845, 2970
BsrI ACTGG 9 cut(s) 391, 563, 1089, 1357, 1874, 2448, 3511, 3520, 4312
BssECI CCNNGG 7 cut(s) 45, 401, 676, 751, 819, 2524, 3153
BssNI GRCGYC 1 cut(s) 1901
BssT1I CCWWGG 4 cut(s) 401, 751, 819, 2524
Bst2UI CCWGG 4 cut(s) 312, 396, 453, 2297
Bst6I CTCTTC 9 cut(s) 1536, 1681, 2067, 2375, 3090, 3303, 4047, 4260, 4262
BstACI GRCGYC 1 cut(s) 1901
BstAFI CTTAAG 1 cut(s) 2222
BstAUI TGTACA 2 cut(s) 845, 2970
BstC8I GCNNGC 9 cut(s) 1533, 1659, 2146, 2150, 2418, 2559, 2770, 3784, 4111
BstDSI CCRYGG 4 cut(s) 401, 676, 2524, 3153
BstEII GGTNACC 1 cut(s) 941
BstENI CCTNNNNNAGG 2 cut(s) 101, 3612
BstFNI CGCG 2 cut(s) 41, 3198
BstH2I RGCGCY 2 cut(s) 1904, 2756
BstHHI GCGC 3 cut(s) 1903, 2755, 3200
BstMAI GTCTC 7 cut(s) 578, 1316, 2132, 2365, 2738, 2766, 4298
BstMCI CGRYCG 3 cut(s) 45, 75, 4236
BstMWI GCNNNNNNNGC 5 cut(s) 713, 2246, 3410, 3426, 3940
BstNI CCWGG 4 cut(s) 312, 396, 453, 2297
BstNSI RCATGY 7 cut(s) 70, 538, 896, 1333, 1535, 1866, 2311
BstPAI GACNNNNGTC 2 cut(s) 1275, 3867
BstPI GGTNACC 1 cut(s) 941
BstSCI CCNGG 5 cut(s) 310, 394, 451, 1896, 2295
BstSFI CTRYAG 7 cut(s) 330, 695, 1806, 2748, 3478, 3645, 3741
BstSLI GKGCMC 3 cut(s) 534, 581, 3527
BstUI CGCG 2 cut(s) 41, 3198
BstV2I GAAGAC 3 cut(s) 3059, 3552, 3988
BstX2I RGATCY 2 cut(s) 682, 2545
BstYI RGATCY 2 cut(s) 682, 2545
BstZI CGGCCG 1 cut(s) 42
Bsu15I ATCGAT 1 cut(s) 75
Bsu36I CCTNAGG 2 cut(s) 2316, 4047
BsuI GTATCC 3 cut(s) 184, 276, 3391
BsuRI GGCC 5 cut(s) 44, 2233, 2416, 2855, 3969
BsuTUI ATCGAT 1 cut(s) 75
BtgI CCRYGG 4 cut(s) 401, 676, 2524, 3153
Cac8I GCNNGC 9 cut(s) 1533, 1659, 2146, 2150, 2418, 2559, 2770, 3784, 4111
CaiI CAGNNNCTG 2 cut(s) 437, 3224
CciI TCATGA 1 cut(s) 3367
CfoI GCGC 3 cut(s) 1903, 2755, 3200
Cfr13I GGNCC 9 cut(s) 308, 398, 449, 824, 2035, 2293, 2608, 2853, 4129
ClaI ATCGAT 1 cut(s) 75
DinI GGCGCC 1 cut(s) 1902
DraI TTTAAA 2 cut(s) 222, 3585
DrdI GACNNNNNNGTC 1 cut(s) 1330
DriI GACNNNNNGTC 1 cut(s) 4311
DseDI GACNNNNNNGTC 1 cut(s) 1330
EaeI YGGCCR 1 cut(s) 42
EagI CGGCCG 1 cut(s) 42
Eam1104I CTCTTC 9 cut(s) 1536, 1681, 2067, 2375, 3090, 3303, 4047, 4260, 4262
Eam1105I GACNNNNNGTC 1 cut(s) 4311
EarI CTCTTC 9 cut(s) 1536, 1681, 2067, 2375, 3090, 3303, 4047, 4260, 4262
EclXI CGGCCG 1 cut(s) 42
Eco130I CCWWGG 4 cut(s) 401, 751, 819, 2524
Eco147I AGGCCT 1 cut(s) 2233
Eco32I GATATC 2 cut(s) 1195, 2202
Eco47I GGWCC 8 cut(s) 308, 398, 449, 824, 2035, 2293, 2608, 4129
Eco47III AGCGCT 1 cut(s) 2754
Eco52I CGGCCG 1 cut(s) 42
Eco81I CCTNAGG 2 cut(s) 2316, 4047
Eco88I CYCGRG 2 cut(s) 101, 1769
Eco91I GGTNACC 1 cut(s) 941
EcoNI CCTNNNNNAGG 2 cut(s) 101, 3612
EcoO109I RGGNCCY 3 cut(s) 2035, 2608, 2853
EcoO65I GGTNACC 1 cut(s) 941
EcoRI GAATTC 4 cut(s) 775, 1252, 1346, 3146
EcoRII CCWGG 4 cut(s) 310, 394, 451, 2295
EcoRV GATATC 2 cut(s) 1195, 2202
EcoT14I CCWWGG 4 cut(s) 401, 751, 819, 2524
EcoT22I ATGCAT 2 cut(s) 1832, 3491
EgeI GGCGCC 1 cut(s) 1902
EheI GGCGCC 1 cut(s) 1902
ErhI CCWWGG 4 cut(s) 401, 751, 819, 2524
FalI AAGNNNNNCTT 8 cut(s) 2404, 2436, 3069, 3101, 3369, 3401, 3939, 3971
FaqI GGGAC 5 cut(s) 837, 2427, 2594, 2911, 3808
FauNDI CATATG 2 cut(s) 1159, 2679
FbaI TGATCA 3 cut(s) 1960, 2179, 3289
FblI GTMKAC 1 cut(s) 2520
GlaI GCGC 3 cut(s) 1902, 2754, 3199
GsuI CTGGAG 2 cut(s) 1106, 2265
HaeII RGCGCY 2 cut(s) 1904, 2756
HaeIII GGCC 5 cut(s) 44, 2233, 2416, 2855, 3969
HapII CCGG 5 cut(s) 125, 171, 1898, 3761, 3966
HhaI GCGC 3 cut(s) 1903, 2755, 3200
Hin1I GRCGYC 1 cut(s) 1901
Hin6I GCGC 3 cut(s) 1901, 2753, 3198
HinP1I GCGC 3 cut(s) 1901, 2753, 3198
HincII GTYRAC 3 cut(s) 607, 1285, 4218
HindII GTYRAC 3 cut(s) 607, 1285, 4218
HindIII AAGCTT 4 cut(s) 285, 3282, 3359, 3402
HpaII CCGG 5 cut(s) 125, 171, 1898, 3761, 3966
HphI GGTGA 4 cut(s) 879, 953, 3284, 3521
Hpy99I CGWCG 1 cut(s) 59
HpyCH4IV ACGT 5 cut(s) 609, 1403, 2878, 3310, 3797
HpyF10VI GCNNNNNNNGC 5 cut(s) 713, 2246, 3410, 3426, 3940
HpySE526I ACGT 5 cut(s) 609, 1403, 2878, 3310, 3797
Hsp92I GRCGYC 1 cut(s) 1901
HspAI GCGC 3 cut(s) 1901, 2753, 3198
KasI GGCGCC 1 cut(s) 1900
Kpn2I TCCGGA 1 cut(s) 124
Ksp22I TGATCA 3 cut(s) 1960, 2179, 3289
LguI GCTCTTC 3 cut(s) 2067, 2375, 4260
LmnI GCTCC 3 cut(s) 204, 1393, 2246
MaeII ACGT 5 cut(s) 609, 1403, 2878, 3310, 3797
MaeIII GTNAC 9 cut(s) 895, 941, 1046, 1784, 1892, 1931, 3063, 3494, 4201
MfeI CAATTG 6 cut(s) 1132, 2009, 2208, 2592, 2916, 4164
MflI RGATCY 2 cut(s) 682, 2545
MhlI GDGCHC 4 cut(s) 534, 581, 3527, 4177
Mly113I GGCGCC 1 cut(s) 1901
MlyI GAGTC 8 cut(s) 1004, 1265, 1781, 2768, 3659, 3830, 4186, 4315
MmeI TCCRAC 4 cut(s) 1906, 2256, 2767, 3041
Mph1103I ATGCAT 2 cut(s) 1832, 3491
MroI TCCGGA 1 cut(s) 124
MroXI GAANNNNTTC 1 cut(s) 2061
MslI CAYNNNNRTG 6 cut(s) 770, 1355, 1861, 1973, 2119, 2355
MspA1I CMGCKG 5 cut(s) 239, 956, 1661, 3221, 3786
MspCI CTTAAG 1 cut(s) 2222
MspI CCGG 5 cut(s) 125, 171, 1898, 3761, 3966
MspR9I CCNGG 5 cut(s) 312, 396, 453, 1898, 2297
MunI CAATTG 6 cut(s) 1132, 2009, 2208, 2592, 2916, 4164
Mva1269I GAATGC 2 cut(s) 2917, 3039
MvaI CCWGG 4 cut(s) 312, 396, 453, 2297
MvnI CGCG 2 cut(s) 41, 3198
MwoI GCNNNNNNNGC 5 cut(s) 713, 2246, 3410, 3426, 3940
NarI GGCGCC 1 cut(s) 1901
NciI CCSGG 1 cut(s) 1898
NcoI CCATGG 2 cut(s) 401, 2524
NdeI CATATG 2 cut(s) 1159, 2679
NheI GCTAGC 2 cut(s) 2144, 2557
NmeAIII GCCGAG 2 cut(s) 70, 141
NmuCI GTSAC 2 cut(s) 941, 3494
NsiI ATGCAT 2 cut(s) 1832, 3491
NspI RCATGY 7 cut(s) 70, 538, 896, 1333, 1535, 1866, 2311
OliI CACNNNNGTG 2 cut(s) 1355, 1973
PaeI GCATGC 1 cut(s) 1535
PaeR7I CTCGAG 1 cut(s) 101
PagI TCATGA 1 cut(s) 3367
PceI AGGCCT 1 cut(s) 2233
PciI ACATGT 5 cut(s) 66, 534, 892, 1329, 1862
PciSI GCTCTTC 3 cut(s) 2067, 2375, 4260
PcsI WCGNNNNNNNCGW 3 cut(s) 572, 2546, 2875
PctI GAATGC 2 cut(s) 2917, 3039
PdmI GAANNNNTTC 1 cut(s) 2061
PflMI CCANNNNNTGG 2 cut(s) 2072, 2423
Ple19I CGATCG 1 cut(s) 75
PleI GAGTC 8 cut(s) 1003, 1265, 1780, 2767, 3658, 3829, 4186, 4315
PluTI GGCGCC 1 cut(s) 1904
PpsI GAGTC 8 cut(s) 1003, 1265, 1780, 2767, 3658, 3829, 4186, 4315
PpuMI RGGWCCY 2 cut(s) 2035, 2608
PscI ACATGT 5 cut(s) 66, 534, 892, 1329, 1862
PshAI GACNNNNGTC 2 cut(s) 1275, 3867
PshBI ATTAAT 1 cut(s) 3108
Psp5II RGGWCCY 2 cut(s) 2035, 2608
Psp6I CCWGG 4 cut(s) 310, 394, 451, 2295
PspEI GGTNACC 1 cut(s) 941
PspGI CCWGG 4 cut(s) 310, 394, 451, 2295
PspPI GGNCC 9 cut(s) 308, 398, 449, 824, 2035, 2293, 2608, 2853, 4129
PspPPI RGGWCCY 2 cut(s) 2035, 2608
PstI CTGCAG 4 cut(s) 334, 699, 1810, 2752
PstNI CAGNNNCTG 2 cut(s) 437, 3224
PsuI RGATCY 2 cut(s) 682, 2545
PvuI CGATCG 1 cut(s) 75
PvuII CAGCTG 5 cut(s) 239, 956, 1661, 3221, 3786
RseI CAYNNNNRTG 6 cut(s) 770, 1355, 1861, 1973, 2119, 2355
SapI GCTCTTC 3 cut(s) 2067, 2375, 4260
Sau96I GGNCC 9 cut(s) 308, 398, 449, 824, 2035, 2293, 2608, 2853, 4129
SbfI CCTGCAGG 1 cut(s) 699
ScaI AGTACT 1 cut(s) 1396
SchI GAGTC 8 cut(s) 1004, 1265, 1781, 2768, 3659, 3830, 4186, 4315
ScrFI CCNGG 5 cut(s) 312, 396, 453, 1898, 2297
SdaI CCTGCAGG 1 cut(s) 699
SduI GDGCHC 4 cut(s) 534, 581, 3527, 4177
SfcI CTRYAG 7 cut(s) 330, 695, 1806, 2748, 3478, 3645, 3741
SfoI GGCGCC 1 cut(s) 1902
Sfr274I CTCGAG 1 cut(s) 101
SinI GGWCC 8 cut(s) 308, 398, 449, 824, 2035, 2293, 2608, 4129
SlaI CTCGAG 1 cut(s) 101
SmiMI CAYNNNNRTG 6 cut(s) 770, 1355, 1861, 1973, 2119, 2355
SpeI ACTAGT 1 cut(s) 623
SphI GCATGC 1 cut(s) 1535
Sse8387I CCTGCAGG 1 cut(s) 699
SseBI AGGCCT 1 cut(s) 2233
SspDI GGCGCC 1 cut(s) 1900
SspI AATATT 3 cut(s) 737, 1372, 3253
StuI AGGCCT 1 cut(s) 2233
StyD4I CCNGG 5 cut(s) 310, 394, 451, 1896, 2295
StyI CCWWGG 4 cut(s) 401, 751, 819, 2524
TaiI ACGT 5 cut(s) 612, 1406, 2881, 3313, 3800
TatI WGTACW 5 cut(s) 845, 1394, 2970, 3975, 4117
TauI GCSGC 3 cut(s) 44, 2461, 3072
TseFI GTSAC 2 cut(s) 941, 3494
Tsp45I GTSAC 2 cut(s) 941, 3494
TspGWI ACGGA 6 cut(s) 564, 596, 693, 2538, 3170, 3553
Van91I CCANNNNNTGG 2 cut(s) 2072, 2423
Vha464I CTTAAG 1 cut(s) 2222
VneI GTGCAC 1 cut(s) 577
VpaK11BI GGWCC 8 cut(s) 308, 398, 449, 824, 2035, 2293, 2608, 4129
VspI ATTAAT 1 cut(s) 3108
XagI CCTNNNNNAGG 2 cut(s) 101, 3612
XapI RAATTY 9 cut(s) 775, 925, 1207, 1252, 1346, 1522, 2996, 3122, 3146
XbaI TCTAGA 2 cut(s) 868, 2254
XceI RCATGY 7 cut(s) 70, 538, 896, 1333, 1535, 1866, 2311
XhoI CTCGAG 1 cut(s) 101
XmiI GTMKAC 1 cut(s) 2520
XmnI GAANNNNTTC 1 cut(s) 2061
ZrmI AGTACT 1 cut(s) 1396
Zsp2I ATGCAT 2 cut(s) 1832, 3491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.