Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0004421

Physical Location & Seq

Genomic Coordinates Reverse (-)

38746752 .. 38755015

Gene Structure 8264 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1300200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4164 bp

ATGTTGAAGGCGGAGGACTGCTCGAATTGGGGAGAGTTACCGGCCGTCGATGGATTTGCAGGCGAGGAATTTGACGAAGACGAGGAGATGTGCATAGATGTCGACACGCTCTATCGCCTTCTCGATGAAGAACCCACGAGTTGTCCAGAGGATTTGTCGTTGGAAAATCAATTGGACGCTGAAGAAGTTTCCGATGCTCAGCTCCTAAGTGGATCCCAAGCAGTAAAAGAAGAACCACCAATAGAAACTGATTTAATGGGTCCAGGTGCGTGGAATCATCCGTACAGCTCAGAAGCTTCGCACTCTGGGGCAGGGGGTCCTGATGGCTCTTTTGATTATAAAGGGAACCATGAAATGAATTTTGATTACAAAGAAATGCAACCATCAGCAAACACTGGCTCTCTGGGGCATACATACTCTATGAGTCTTAAAGACTGGATTTCCTTGGTTCCAAGCGATGAGACTTGCTTTACAGAGAGGGTTGCAGTTTCACAGCCATGTAGTATGTCGTCTAGTATTTCTGAAGGGCATGCCAACCATGTTTTGGACCACAGAGATCTGAATGTCCTGCAGAGGGAAGTTGATGTGCCTGGGAAAGCTGAATCTGAATATGCTTTGCAGTGCCTCGATATTGAGAACATGGGCTTACGTTCCAGAACCTATTTCACTTTGGGACAAAATACCACGGAAACCTTAGGGCCATCTGAAAATAATTCCTGCACTTCTATGGAAATATGCTCTACTCTCTGTCAAAATTCTGATGTTTTCAGTGACCCTTATACTGCTATGTGTGGCATGAGCTTAGATAATACATATTTCACTGATGTCTTAATGCATCATAGTCCTACCAGTTATTATTTTCCGATTAATGAAGAAATGATGGCTAATGTGAAGGATGAATCAGGGGAGTTTCCAGCTGATAGTTCATGCTCAACTAGCAAGATGAATTTAAATGGTCAGGAGGTGATAACTGGTAGATCTGTTTTTGAGCCGTCAATGATTGATGTTTTAGATGGAAAGGAGTGGAATTTGAGATATGGTAATTGTATGTCAGCAATCAGTGGAAATTCTTCATTCGATGCTGACTCTTTTCCTGTTGACAATAAGGCATCAGTTGAGCCTTTGAGTTCTACTCAGACATACATGTCAAGCAAAAAGGAAAGTATTGGTGTGAAGGATGAAAAGAATGATGAGTTTGTTGCTCCAAGTAGTGTTATGTGTCATTCTTTTCGAGGCATGGATGAAGCTGTCAGTAGACAGTCTTCTTTTAGTGCTCATGATCACTTTTTTGAGAAAGACTTCAAGCAGTCTGGTTTTGGTATATCAACACAGAATCTTGGTAACTCATTGGATAATGAAGAAGTCACGATTGGTGCATGTAAGAGAGCTTGTCTTTCTCAAGATATTAAGCATGGAAGTTCCACATCCCCTGTTGATGGACAATCTGTGAATTCAAATGCCTATTCTCAAGGTGCTCAGTCATTCACTTCATGCAAGAGTCAGGGTTACATGAAGGATATAGTCACTCATTCTAAGAGCATGCACCCTTCGAAAGTCAGCCCTGAATCAATTCATAGTAACTTCTCTGACAAATCCCCTGCAGATGATGACGTTGATGTATGTATTATTGAACATATCAGTCATCCTGCACCCTCAAGTCGCTCTTCAGTGGTTAGCAATACTAATTACGCTGCGCCCATGAAACAGTCTCCAGCACTTGGAAATACTGTTGTTAATTCACAACAATTGATGCCGAGTGATCATTACGGAGGAGTGGGAGGCATGAGATTCAAGACACGAGATGAACAATTGGTTTTGAGAGTAGCATTGCAGGACCTTTCACAGCCCAAGTCAGAAGCTATTCCACCAGATGGACTTTTGGCAGTTCCTCTGTTACGACATCAGAGAATTGCATTGTCATGGATGGTTCAGAAAGAGACAGCTAGTTTGCACTGTTCTGGAGGAATTCTTGCGGATGATCAGGGATTAGGAAAGACAATATCGACAATTGCACTTATACTTAAGGAAAGGCCTTCATATGGAGCTTGTCAAGATGTAAAAAAGCAAAAGCTGGAAACTCTGGATTTGGACAATGATAATGATATGCATTTTGAGATTGGTGGAAGGAAGCAACATGCTGATGCCCATGAAGTTATTTCAAATGGAACTCCAAACGAGAGCATAAAACCTTTGATACAAGCAAAGGGAAGGCCAGCTGCTGGAACACTTGTTGTTTGTCCCACGAGTGTCCTCCGGCAATGGGCCGAGGAGTTGCAGAATAAGGTAACTGAAAAAGCTAATCTCTCGGTGCTAGTTTACCATGGAAGCAACCGGACAAGGGATCCTTTGGAGCTGGCTAAGCATGATGTTGTCCTCACAACTTATTCAATTGTCAGCATGGAGGTTCCTAAGCAACCTCTTGTTGATGACGATGATGAGGAGAAAGGAAACCGAGACGAGTACGATGGTCCACATTCCAGTAAGAAAAGGAAATACCCTCAAAGTTCCTCAAATAAAGGTTCAAAAGGTAAGAAGGGATTAGAAAGTGCTGTGCTCGAGTCTGTTGCTCGCCCTCTTGCTAAGGTTGCATGGTTTAGGGTTGTTCTGGATGAGGCTCAAAGTATTAAGAATCACAGAACTCAAGTAGCTAGGGCCTGTTGGGGTCTTCGCGCTAAACGTAGGTGGTGCTTATCAGGCACTCCTATCCAGAATGCAATTGATGACCTTTATAGCTACTTCAGATTTCTCAAATATGACCCTTATGCTGTATACAAGTCATTTATCTCTACAATTAAGGTCCCAATTAGTAGGAATCCAACAAAAGGGTACAGAAAACTTCAAGCTGTACTGAAGACAATTATGTTACGCCGCACAAAAGGCACACTTCTTGATGGACAACCAATTATTAATTTACCACCTAAATCCATAGAATTGAAAAGAGTGGAGTTTTCGAAGGAAGAACGAGATTTCTACTCAAGGCTAGAGGCTGATTCACGTGCTCAGTTTGAAGAATATGCAGCTGCGGGAACTGTCAAACAAAATTATGTTAACATTTTGTTGATGCTTTTGCGTCTTCGACAAGCTTGTGATCACCCTCTTCTTGTTAGACGGTATGATTCAAATTCTTTATGGAGATCTTCAGTTGAGAAGGCAAAGAAACTTCCTCTTGATAAACAACAATCCCTTCTGAATTGCTTAGAAGCTTCTTTGGCGATATGTGGCATCTGCAGTGATCCACCTGAAGACGCTGTTGTTTCCGAATGTGGTCATGTCTTCTGTAGCCAATGCATTAATGAACATCTCACCGGTGATGATAACCAGTGCCCAAACACAAATTGCAAAGTTCGACTCCACGCTTCTTCAGTGTTTTCAAAAGCCACTTTGAACAGTTCTCTTTCTGACCAGACTAATCCAGAAAGCATTGGTTCTGAAGTTTTTGATGCTGTAGAGACTTTCTACGAGGATCGCTCATACAATTCTTCCAAGATTAAGGCTGCTCTTGAGGTTCTGTGCTCAATGTGTAAACCACAGGGTTGCATTTCTGGAAATAGTTGCTTAGAGGACAGAGTTGATCGAAATGCCAGCTGTTCTGAAAATTCTTCCGATATTTCTGTGGTTGAACCACTTGAGGACGTTCCTAAGAGTCAGAAATTGGATGTGGTGGTGGGCTCTAAGAATACAAAGAAGGTAGTTAGAGAGAAAGCAATAGTCTTTTCACAGTGGACGAGGATGCTGGACTTGCTTGAAGCATGTCTTAAAACATCTTCCATTGAGTACAGAAGACTCGATGGAACAATGTCAGTAACAGCTAGGGATAAAGCTGTCAAGGATTTTAACACCCTTCCAGAGGTGACAGTCATGATTATGTCTTTGAAAGCAGCTAGTCTTGGTCTCAACATGGTTGCAGCTTGTCATGTTCTTCTTTTGGACCTTTGGTGGAATCCTACCACAGAAGATCAAGCAATTGATAGAGCTCATCGGATTGGGCAGACCCGTCCTGTTACAGTTTTGCGTTTGACTGTTAAAGACACCGTTGAAGATCGTATTTTAGCCCTTCAGCAAAAGAAGAGAGAGATGGTTGCGTCTGCATTTGGAGAGGATAAAACGGGTGGTGGACAGACTCGCCTGACAGTAGAGGATCTTAAGTACCTGTTTATGATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000724 GO:0000725 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009292 GO:0009294 GO:0009987 GO:0033554 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0044764 GO:0045003 GO:0046483 GO:0050896 GO:0051704 GO:0051716 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Helicase_C SNF2_N zf-C3HC4 zf-C3HC4_2

Protein Analysis

1388

Amino Acids

153.38

Weight (kDa)

5.28

Isoelectric Point (pI)

52.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SNF2-rel_dom	PF00176	634 - 1032	3.9e-66	SNF2-related domain
zf-C3HC4	PF00097	1073 - 1109	2.2e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2	PF13923	1073 - 1109	1.3e-07	Zinc finger, C3HC4 type (RING finger)
Helicase_C	PF00271	1229 - 1329	4.3e-20	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0004421)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11100 AT1G11100 AT1G11100 AT1G11100 AT1G11100 AT1G61140 AT1G61140 AT1G61140
fragaria_vesca	FvH4_3g04580 FvH4_3g04580 FvH4_3g04580 FvH4_3g04580 FvH4_3g04580
malus_domestica	MD05G1322200.v1.1 MD10G1300200.v1.1
prunus_persica	Prupe.4G040600_v2.0.a1 Prupe.4G040600_v2.0.a1
pyrus_communis	pycom05g29650 pycom10g25290
rosa_chinensis	RchiOBHm_Chr5g0007521
rosa_laevigata	RLG00000031464
rosa_multiflora	Rmu_co8522595.1_g000001 Rmu_ssc0000182.1_g000011
rosa_roxburghii	Rroxscaffold_1G00068890
rosa_rugosa	Rorug04G0427800 Rorug04G0427900 Rorug04G0428000
rosa_samantha	Rh5AG055800 Rh5BG053400 Rh5CG063200 Rh5DG052400
rosa_wichuraiana	Rw5G005420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	339
AasI	GACNNNNNNGTC	1 cut(s)	1144
AccB7I	CCANNNNNTGG	4 cut(s)	544, 1718, 1871, 2219
AccI	GTMKAC	3 cut(s)	102, 1255, 2769
AccII	CGCG	1 cut(s)	2670
AciI	CCGC	4 cut(s)	11, 1973, 2869, 3023
AclWI	GGATC	7 cut(s)	207, 220, 2336, 2349, 3227, 3471, 4146
AcoI	YGGCCR	1 cut(s)	42
AcvI	CACGTG	1 cut(s)	2996
AfaI	GTAC	6 cut(s)	284, 2462, 2828, 2847, 3776, 4148
AflII	CTTAAG	2 cut(s)	2021, 4142
AflIII	ACRYGT	1 cut(s)	1143
AgeI	ACCGGT	1 cut(s)	3305
AjnI	CCWGG	2 cut(s)	262, 589
AjuI	GAANNNNNNNTTGG	2 cut(s)	1353, 1385
Alw21I	GWGCWC	6 cut(s)	1276, 1477, 2556, 3001, 3515, 3976
Alw26I	GTCTC	6 cut(s)	455, 1713, 1931, 2448, 3443, 3896
AlwI	GGATC	7 cut(s)	207, 220, 2336, 2349, 3227, 3471, 4146
AlwNI	CAGNNNCTG	1 cut(s)	2219
Ama87I	CYCGRG	1 cut(s)	2555
AoxI	GGCC	6 cut(s)	42, 698, 2030, 2210, 2262, 2652
ApeKI	GCWGC	7 cut(s)	1691, 2216, 3017, 3020, 3494, 3878, 3905
ArsI	GACNNNNNNTTYG	2 cut(s)	1930, 1962
AseI	ATTAAT	3 cut(s)	867, 2907, 3291
AsiGI	ACCGGT	1 cut(s)	3305
Asp700I	GAANNNNTTC	4 cut(s)	1069, 1298, 1569, 1860
AspLEI	GCGC	2 cut(s)	1696, 2672
AsuHPI	GGTGA	5 cut(s)	976, 3083, 3295, 3320, 3862
AsuII	TTCGAA	2 cut(s)	1550, 2951
AvaI	CYCGRG	1 cut(s)	2555
AvaII	GGWCC	7 cut(s)	260, 317, 547, 1834, 2468, 2797, 3928
AxyI	CCTNAGG	1 cut(s)	694
BaeGI	GKGCMC	1 cut(s)	3326
BamHI	GGATCC	2 cut(s)	212, 2341
BanII	GRGCYC	2 cut(s)	3671, 3976
BarI	GAAGNNNNNNTAC	2 cut(s)	1246, 1278
BauI	CACGAG	3 cut(s)	136, 1797, 2242
BbrPI	CACGTG	1 cut(s)	2996
BbsI	GAAGAC	8 cut(s)	84, 1254, 2657, 2858, 3065, 3249, 3265, 3787
Bbv12I	GWGCWC	6 cut(s)	1276, 1477, 2556, 3001, 3515, 3976
BbvI	GCAGC	7 cut(s)	1678, 2203, 3007, 3029, 3481, 3890, 3917
BceAI	ACGGC	2 cut(s)	29, 976
BcgI	CGANNNNNNTGC	6 cut(s)	38, 72, 82, 116, 2546, 2580
BciT130I	CCWGG	2 cut(s)	264, 591
BclI	TGATCA	4 cut(s)	1279, 1759, 1978, 3088
BcoDI	GTCTC	6 cut(s)	455, 1713, 1931, 2448, 3443, 3896
BfaI	CTAG	8 cut(s)	513, 936, 1944, 2312, 2649, 2981, 3810, 3882
BfmI	CTRYAG	5 cut(s)	569, 1599, 3226, 3277, 3444
BfrI	CTTAAG	2 cut(s)	2021, 4142
BglII	AGATCT	3 cut(s)	556, 977, 3134
BisI	GCNGC	8 cut(s)	1692, 2217, 2869, 3018, 3021, 3495, 3879, 3906
BlpI	GCTNAGC	2 cut(s)	198, 2358
BlsI	GCNGC	8 cut(s)	1693, 2218, 2870, 3019, 3022, 3496, 3880, 3907
Bme1390I	CCNGG	2 cut(s)	264, 591
Bme18I	GGWCC	7 cut(s)	260, 317, 547, 1834, 2468, 2797, 3928
BmeT110I	CYCGRG	1 cut(s)	2555
BmiI	GGNNCC	8 cut(s)	214, 261, 318, 347, 450, 2343, 2406, 2799
BmrFI	CCNGG	2 cut(s)	264, 591
BpiI	GAAGAC	8 cut(s)	84, 1254, 2657, 2858, 3065, 3249, 3265, 3787
BplI	GAGNNNNNCTC	5 cut(s)	37, 1117, 1149, 3452, 3484
BpmI	CTGGAG	2 cut(s)	1695, 1980
Bpu10I	CCTNAGC	2 cut(s)	2409, 2580
Bpu1102I	GCTNAGC	2 cut(s)	198, 2358
Bpu14I	TTCGAA	2 cut(s)	1550, 2951
BpuEI	CTTGAG	7 cut(s)	1383, 1452, 1639, 2625, 2959, 3521, 3647
BsaAI	YACGTR	1 cut(s)	2996
BsaI	GGTCTC	1 cut(s)	3896
BsaJI	CCNNGG	5 cut(s)	444, 590, 684, 2265, 2320
BsaWI	WCCGGW	2 cut(s)	2331, 3305
Bse118I	RCCGGY	2 cut(s)	40, 3305
Bse1I	ACTGG	6 cut(s)	400, 440, 849, 976, 2478, 3319
Bse21I	CCTNAGG	1 cut(s)	694
Bse3DI	GCAATG	2 cut(s)	1826, 2264
BseBI	CCWGG	2 cut(s)	264, 591
BseDI	CCNNGG	5 cut(s)	444, 590, 684, 2265, 2320
BseMI	GCAATG	2 cut(s)	1826, 2264
BseMII	CTCAG	5 cut(s)	212, 303, 1148, 1490, 3014
BseNI	ACTGG	6 cut(s)	400, 440, 849, 976, 2478, 3319
BseRI	GAGGAG	4 cut(s)	98, 1785, 2282, 2453
BseSI	GKGCMC	1 cut(s)	3326
BseX3I	CGGCCG	1 cut(s)	42
BseXI	GCAGC	7 cut(s)	1678, 2203, 3007, 3029, 3481, 3890, 3917
BsgI	GTGCAG	2 cut(s)	703, 1632
Bsh1236I	CGCG	1 cut(s)	2670
Bsh1285I	CGRYCG	1 cut(s)	45
BshFI	GGCC	6 cut(s)	44, 700, 2032, 2212, 2264, 2654
BshTI	ACCGGT	1 cut(s)	3305
BsiEI	CGRYCG	1 cut(s)	45
BsiHKAI	GWGCWC	6 cut(s)	1276, 1477, 2556, 3001, 3515, 3976
BsiHKCI	CYCGRG	1 cut(s)	2555
BsiSI	CCGG	4 cut(s)	41, 2254, 2332, 3306
BslFI	GGGAC	3 cut(s)	687, 2223, 2783
BsmAI	GTCTC	6 cut(s)	455, 1713, 1931, 2448, 3443, 3896
BsmBI	CGTCTC	1 cut(s)	2448
BsmFI	GGGAC	3 cut(s)	687, 2223, 2783
BsmI	GAATGC	1 cut(s)	2716
BsnI	GGCC	6 cut(s)	44, 700, 2032, 2212, 2264, 2654
Bso31I	GGTCTC	1 cut(s)	3896
BsoBI	CYCGRG	1 cut(s)	2555
Bsp119I	TTCGAA	2 cut(s)	1550, 2951
Bsp1286I	GDGCHC	8 cut(s)	1276, 1477, 2556, 3001, 3326, 3515, 3671, 3976
Bsp1720I	GCTNAGC	2 cut(s)	198, 2358
Bsp19I	CCATGG	1 cut(s)	2320
BspACI	CCGC	4 cut(s)	11, 1973, 2869, 3023
BspANI	GGCC	6 cut(s)	44, 700, 2032, 2212, 2264, 2654
BspCNI	CTCAG	5 cut(s)	211, 302, 1147, 1489, 3013
BspFNI	CGCG	1 cut(s)	2670
BspHI	TCATGA	2 cut(s)	1276, 3858
BspLI	GGNNCC	8 cut(s)	214, 261, 318, 347, 450, 2343, 2406, 2799
BspMAI	CTGCAG	3 cut(s)	573, 1603, 3230
BspPI	GGATC	7 cut(s)	207, 220, 2336, 2349, 3227, 3471, 4146
BspQI	GCTCTTC	1 cut(s)	1669
BspT104I	TTCGAA	2 cut(s)	1550, 2951
BspTI	CTTAAG	2 cut(s)	2021, 4142
BspTNI	GGTCTC	1 cut(s)	3896
BsrDI	GCAATG	2 cut(s)	1826, 2264
BsrFI	RCCGGY	2 cut(s)	40, 3305
BsrI	ACTGG	6 cut(s)	400, 440, 849, 976, 2478, 3319
BssAI	RCCGGY	2 cut(s)	40, 3305
BssECI	CCNNGG	5 cut(s)	444, 590, 684, 2265, 2320
BssNAI	GTATAC	1 cut(s)	2770
BssSI	CACGAG	3 cut(s)	136, 1797, 2242
BssT1I	CCWWGG	2 cut(s)	444, 2320
Bst1107I	GTATAC	1 cut(s)	2770
Bst2BI	CACGAG	3 cut(s)	136, 1797, 2242
Bst2UI	CCWGG	2 cut(s)	264, 591
Bst6I	CTCTTC	3 cut(s)	1669, 3102, 4061
BstAFI	CTTAAG	2 cut(s)	2021, 4142
BstBAI	YACGTR	1 cut(s)	2996
BstBI	TTCGAA	2 cut(s)	1550, 2951
BstC8I	GCNNGC	7 cut(s)	61, 531, 1541, 2214, 2355, 2569, 3583
BstDSI	CCRYGG	2 cut(s)	684, 2320
BstENI	CCTNNNNNAGG	1 cut(s)	3845
BstFNI	CGCG	1 cut(s)	2670
BstHHI	GCGC	2 cut(s)	1696, 2672
BstMAI	GTCTC	6 cut(s)	455, 1713, 1931, 2448, 3443, 3896
BstMCI	CGRYCG	1 cut(s)	45
BstMWI	GCNNNNNNNGC	7 cut(s)	936, 2359, 2585, 2694, 2877, 3209, 3739
BstNI	CCWGG	2 cut(s)	264, 591
BstNSI	RCATGY	6 cut(s)	533, 1147, 1380, 1543, 2138, 3753
BstSCI	CCNGG	2 cut(s)	262, 589
BstSFI	CTRYAG	5 cut(s)	569, 1599, 3226, 3277, 3444
BstSLI	GKGCMC	1 cut(s)	3326
BstUI	CGCG	1 cut(s)	2670
BstV1I	GCAGC	7 cut(s)	1678, 2203, 3007, 3029, 3481, 3890, 3917
BstV2I	GAAGAC	8 cut(s)	84, 1254, 2657, 2858, 3065, 3249, 3265, 3787
BstX2I	RGATCY	6 cut(s)	212, 556, 977, 2341, 3134, 4138
BstXI	CCANNNNNNTGG	1 cut(s)	270
BstYI	RGATCY	6 cut(s)	212, 556, 977, 2341, 3134, 4138
BstZ17I	GTATAC	1 cut(s)	2770
BstZI	CGGCCG	1 cut(s)	42
Bsu36I	CCTNAGG	1 cut(s)	694
BsuRI	GGCC	6 cut(s)	44, 700, 2032, 2212, 2264, 2654
BtgI	CCRYGG	2 cut(s)	684, 2320
BtgZI	GCGATG	1 cut(s)	471
BtsI	GCAGTG	2 cut(s)	626, 3235
Cac8I	GCNNGC	7 cut(s)	61, 531, 1541, 2214, 2355, 2569, 3583
CaiI	CAGNNNCTG	1 cut(s)	2219
CciI	TCATGA	2 cut(s)	1276, 3858
CfoI	GCGC	2 cut(s)	1696, 2672
Cfr10I	RCCGGY	2 cut(s)	40, 3305
CseI	GACGC	4 cut(s)	185, 3059, 3254, 4071
Csp6I	GTAC	6 cut(s)	283, 2461, 2827, 2846, 3775, 4147
CspAI	ACCGGT	1 cut(s)	3305
CviQI	GTAC	6 cut(s)	283, 2461, 2827, 2846, 3775, 4147
DraI	TTTAAA	1 cut(s)	951
DrdI	GACNNNNNNGTC	1 cut(s)	1144
DseDI	GACNNNNNNGTC	1 cut(s)	1144
EaeI	YGGCCR	1 cut(s)	42
EagI	CGGCCG	1 cut(s)	42
Eam1104I	CTCTTC	3 cut(s)	1669, 3102, 4061
EarI	CTCTTC	3 cut(s)	1669, 3102, 4061
EciI	GGCGGA	1 cut(s)	26
Ecl136II	GAGCTC	1 cut(s)	3974
EclXI	CGGCCG	1 cut(s)	42
Eco130I	CCWWGG	2 cut(s)	444, 2320
Eco147I	AGGCCT	1 cut(s)	2032
Eco24I	GRGCYC	2 cut(s)	3671, 3976
Eco31I	GGTCTC	1 cut(s)	3896
Eco47I	GGWCC	7 cut(s)	260, 317, 547, 1834, 2468, 2797, 3928
Eco52I	CGGCCG	1 cut(s)	42
Eco53kI	GAGCTC	1 cut(s)	3974
Eco72I	CACGTG	1 cut(s)	2996
Eco81I	CCTNAGG	1 cut(s)	694
Eco88I	CYCGRG	1 cut(s)	2555
EcoICRI	GAGCTC	1 cut(s)	3974
EcoNI	CCTNNNNNAGG	1 cut(s)	3845
EcoO109I	RGGNCCY	4 cut(s)	317, 1834, 2652, 2797
EcoRI	GAATTC	2 cut(s)	1450, 1965
EcoRII	CCWGG	2 cut(s)	262, 589
EcoT14I	CCWWGG	2 cut(s)	444, 2320
EcoT22I	ATGCAT	3 cut(s)	837, 2109, 3290
EcoT38I	GRGCYC	2 cut(s)	3671, 3976
ErhI	CCWWGG	2 cut(s)	444, 2320
Esp3I	CGTCTC	1 cut(s)	2448
FalI	AAGNNNNNCTT	8 cut(s)	1648, 1680, 2329, 2361, 2868, 2900, 3738, 3770
FaqI	GGGAC	3 cut(s)	687, 2223, 2783
FauI	CCCGC	1 cut(s)	3016
FauNDI	CATATG	1 cut(s)	2038
FbaI	TGATCA	4 cut(s)	1279, 1759, 1978, 3088
FblI	GTMKAC	3 cut(s)	102, 1255, 2769
Fnu4HI	GCNGC	8 cut(s)	1692, 2217, 2869, 3018, 3021, 3495, 3879, 3906
FriOI	GRGCYC	2 cut(s)	3671, 3976
Fsp4HI	GCNGC	8 cut(s)	1692, 2217, 2869, 3018, 3021, 3495, 3879, 3906
FspBI	CTAG	8 cut(s)	513, 936, 1944, 2312, 2649, 2981, 3810, 3882
GlaI	GCGC	2 cut(s)	1695, 2671
GluI	GCNGC	8 cut(s)	1692, 2217, 2869, 3018, 3021, 3495, 3879, 3906
GsuI	CTGGAG	2 cut(s)	1695, 1980
HaeIII	GGCC	6 cut(s)	44, 700, 2032, 2212, 2264, 2654
HapII	CCGG	4 cut(s)	41, 2254, 2332, 3306
HgaI	GACGC	4 cut(s)	185, 3059, 3254, 4071
HhaI	GCGC	2 cut(s)	1696, 2672
Hin6I	GCGC	2 cut(s)	1694, 2670
HinP1I	GCGC	2 cut(s)	1694, 2670
HincII	GTYRAC	3 cut(s)	103, 1099, 3049
HindII	GTYRAC	3 cut(s)	103, 1099, 3049
HindIII	AAGCTT	3 cut(s)	294, 3081, 3201
HpaI	GTTAAC	1 cut(s)	3049
HpaII	CCGG	4 cut(s)	41, 2254, 2332, 3306
HphI	GGTGA	5 cut(s)	976, 3083, 3295, 3320, 3862
Hpy99I	CGWCG	1 cut(s)	50
HpyCH4IV	ACGT	5 cut(s)	649, 1611, 2677, 2995, 3633
HpyF10VI	GCNNNNNNNGC	7 cut(s)	936, 2359, 2585, 2694, 2877, 3209, 3739
HpySE526I	ACGT	5 cut(s)	649, 1611, 2677, 2995, 3633
HspAI	GCGC	2 cut(s)	1694, 2670
Ksp22I	TGATCA	4 cut(s)	1279, 1759, 1978, 3088
KspAI	GTTAAC	1 cut(s)	3049
LguI	GCTCTTC	1 cut(s)	1669
LmnI	GCTCC	4 cut(s)	207, 1207, 2042, 2350
Lsp1109I	GCAGC	7 cut(s)	1678, 2203, 3007, 3029, 3481, 3890, 3917
MaeI	CTAG	8 cut(s)	513, 936, 1944, 2312, 2649, 2981, 3810, 3882
MaeII	ACGT	5 cut(s)	649, 1611, 2677, 2995, 3633
MfeI	CAATTG	7 cut(s)	170, 1745, 1808, 2007, 2388, 2715, 3963
MflI	RGATCY	6 cut(s)	212, 556, 977, 2341, 3134, 4138
MhlI	GDGCHC	8 cut(s)	1276, 1477, 2556, 3001, 3326, 3515, 3671, 3976
MlyI	GAGTC	8 cut(s)	433, 1079, 1507, 2567, 3342, 3652, 3777, 4114
MmeI	TCCRAC	2 cut(s)	141, 2840
Mph1103I	ATGCAT	3 cut(s)	837, 2109, 3290
MroXI	GAANNNNTTC	4 cut(s)	1069, 1298, 1569, 1860
MslI	CAYNNNNRTG	5 cut(s)	496, 725, 1918, 2139, 3863
MspA1I	CMGCKG	4 cut(s)	917, 2216, 3020, 3585
MspCI	CTTAAG	2 cut(s)	2021, 4142
MspI	CCGG	4 cut(s)	41, 2254, 2332, 3306
MspR9I	CCNGG	2 cut(s)	264, 591
MunI	CAATTG	7 cut(s)	170, 1745, 1808, 2007, 2388, 2715, 3963
Mva1269I	GAATGC	1 cut(s)	2716
MvaI	CCWGG	2 cut(s)	264, 591
MvnI	CGCG	1 cut(s)	2670
MwoI	GCNNNNNNNGC	7 cut(s)	936, 2359, 2585, 2694, 2877, 3209, 3739
NcoI	CCATGG	1 cut(s)	2320
NdeI	CATATG	1 cut(s)	2038
NlaIV	GGNNCC	8 cut(s)	214, 261, 318, 347, 450, 2343, 2406, 2799
NmeAIII	GCCGAG	2 cut(s)	1779, 2290
NmuCI	GTSAC	4 cut(s)	770, 1363, 1522, 3850
NsiI	ATGCAT	3 cut(s)	837, 2109, 3290
NspI	RCATGY	6 cut(s)	533, 1147, 1380, 1543, 2138, 3753
NspV	TTCGAA	2 cut(s)	1550, 2951
PaeI	GCATGC	2 cut(s)	533, 1543
PaeR7I	CTCGAG	1 cut(s)	2555
PagI	TCATGA	2 cut(s)	1276, 3858
PceI	AGGCCT	1 cut(s)	2032
PciI	ACATGT	1 cut(s)	1143
PciSI	GCTCTTC	1 cut(s)	1669
PcsI	WCGNNNNNNNCGW	1 cut(s)	2674
PctI	GAATGC	1 cut(s)	2716
PdmI	GAANNNNTTC	4 cut(s)	1069, 1298, 1569, 1860
PflMI	CCANNNNNTGG	4 cut(s)	544, 1718, 1871, 2219
PinAI	ACCGGT	1 cut(s)	3305
PkrI	GCNGC	8 cut(s)	1693, 2218, 2870, 3019, 3022, 3496, 3880, 3907
PleI	GAGTC	8 cut(s)	432, 1079, 1506, 2566, 3342, 3651, 3777, 4114
PmaCI	CACGTG	1 cut(s)	2996
PmlI	CACGTG	1 cut(s)	2996
PpsI	GAGTC	8 cut(s)	432, 1079, 1506, 2566, 3342, 3651, 3777, 4114
Ppu21I	YACGTR	1 cut(s)	2996
PpuMI	RGGWCCY	3 cut(s)	317, 1834, 2797
PscI	ACATGT	1 cut(s)	1143
PshBI	ATTAAT	3 cut(s)	867, 2907, 3291
PsiI	TTATAA	1 cut(s)	339
Psp124BI	GAGCTC	1 cut(s)	3976
Psp5II	RGGWCCY	3 cut(s)	317, 1834, 2797
Psp6I	CCWGG	2 cut(s)	262, 589
PspCI	CACGTG	1 cut(s)	2996
PspGI	CCWGG	2 cut(s)	262, 589
PspN4I	GGNNCC	8 cut(s)	214, 261, 318, 347, 450, 2343, 2406, 2799
PspPPI	RGGWCCY	3 cut(s)	317, 1834, 2797
PspXI	VCTCGAGB	1 cut(s)	2555
PstI	CTGCAG	3 cut(s)	573, 1603, 3230
PstNI	CAGNNNCTG	1 cut(s)	2219
PsuI	RGATCY	6 cut(s)	212, 556, 977, 2341, 3134, 4138
PvuII	CAGCTG	4 cut(s)	917, 2216, 3020, 3585
RsaI	GTAC	6 cut(s)	284, 2462, 2828, 2847, 3776, 4148
RsaNI	GTAC	6 cut(s)	283, 2461, 2827, 2846, 3775, 4147
RseI	CAYNNNNRTG	5 cut(s)	496, 725, 1918, 2139, 3863
SacI	GAGCTC	1 cut(s)	3976
SalI	GTCGAC	1 cut(s)	101
SapI	GCTCTTC	1 cut(s)	1669
SatI	GCNGC	8 cut(s)	1692, 2217, 2869, 3018, 3021, 3495, 3879, 3906
SchI	GAGTC	8 cut(s)	433, 1079, 1507, 2567, 3342, 3652, 3777, 4114
ScrFI	CCNGG	2 cut(s)	264, 591
SduI	GDGCHC	8 cut(s)	1276, 1477, 2556, 3001, 3326, 3515, 3671, 3976
SfcI	CTRYAG	5 cut(s)	569, 1599, 3226, 3277, 3444
Sfr274I	CTCGAG	1 cut(s)	2555
SfuI	TTCGAA	2 cut(s)	1550, 2951
SgrAI	CRCCGGYG	1 cut(s)	3305
SinI	GGWCC	7 cut(s)	260, 317, 547, 1834, 2468, 2797, 3928
SlaI	CTCGAG	1 cut(s)	2555
SmiI	ATTTAAAT	1 cut(s)	951
SmiMI	CAYNNNNRTG	5 cut(s)	496, 725, 1918, 2139, 3863
SphI	GCATGC	2 cut(s)	533, 1543
SseBI	AGGCCT	1 cut(s)	2032
SsiI	CCGC	4 cut(s)	11, 1973, 2869, 3023
SspMI	CTAG	8 cut(s)	513, 936, 1944, 2312, 2649, 2981, 3810, 3882
SstI	GAGCTC	1 cut(s)	3976
StuI	AGGCCT	1 cut(s)	2032
StyD4I	CCNGG	2 cut(s)	262, 589
StyI	CCWWGG	2 cut(s)	444, 2320
SwaI	ATTTAAAT	1 cut(s)	951
TaiI	ACGT	5 cut(s)	652, 1614, 2680, 2998, 3636
TatI	WGTACW	2 cut(s)	2845, 3774
TauI	GCSGC	1 cut(s)	2871
TseFI	GTSAC	4 cut(s)	770, 1363, 1522, 3850
TseI	GCWGC	7 cut(s)	1691, 2216, 3017, 3020, 3494, 3878, 3905
Tsp45I	GTSAC	4 cut(s)	770, 1363, 1522, 3850
TspGWI	ACGGA	3 cut(s)	270, 701, 1782
Van91I	CCANNNNNTGG	4 cut(s)	544, 1718, 1871, 2219
Vha464I	CTTAAG	2 cut(s)	2021, 4142
VpaK11BI	GGWCC	7 cut(s)	260, 317, 547, 1834, 2468, 2797, 3928
VspI	ATTAAT	3 cut(s)	867, 2907, 3291
XagI	CCTNNNNNAGG	1 cut(s)	3845
XceI	RCATGY	6 cut(s)	533, 1147, 1380, 1543, 2138, 3753
XcmI	CCANNNNNNNNNTGG	1 cut(s)	541
XhoI	CTCGAG	1 cut(s)	2555
XmiI	GTMKAC	3 cut(s)	102, 1255, 2769
XmnI	GAANNNNTTC	4 cut(s)	1069, 1298, 1569, 1860
XspI	CTAG	8 cut(s)	513, 936, 1944, 2312, 2649, 2981, 3810, 3882
Zsp2I	ATGCAT	3 cut(s)	837, 2109, 3290

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.