MD05G1324200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014632
Physical Location & Seq
Forward (+)
45101219 .. 45102538
1320 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1324200.v1.1.491

Sequence Viewer

Length: 1320 bp
ATGGAGCATCAGCATAAAAAGAACGTCGTGAATATTAATGATCTCCCTGAGGACTTATTCGTTCGAATCCTTTCTCGGCTCCCTTGCAACATGGTTTTCCAATGCATGTGCGTGTCCAAGCGTTGGTTCGTGCTCCTCAATAGTTCGTATTTTGTTCGCCAGTTTTCACGTTTCCAAAGGGAATTAGAGCAAAATCCCATCACGGGTACTGTGTTGTTCTCTTGGTATTCATCACGTCACCACAATCGTTGCCTATATCCCAAGCTCCCAAAATCTATAACACACAGATTTTATACTAGGAGTTTCCTTCCCCGGCGTTCTGATGGTGTATTTTACAAATCACTGTTAGTGTCGATGTATAATGACTTAGCTTTGTGCAACAACGAACATGGGGGAAGTTATTACTACATCTGCAATACATACACCATGCAATGGGTTGCTCTTCCGCCATTGAAATTCGAGTGGAGGAGAGATAGAGTAGGATTCATCTGCGATCCTTATATGGAAGATGATAAAAATTTGGAACAAGATGGTACTAGTAGTTGTAACGCTGAATATAGGTGCAGGGTTGTGCTAATTCATCACAAACACAATGCTCTTAAATTAAACATGGTGATGTTTTCTTCCGAGACTGGTGAATGGAGAGAGTTTGTTGTACCATGCCGACCAAAAACTCATTTGGTGCTCTGCGAGTCTTACATGAATTTGGCCCAGCCGGAAGGAGTTGCTTTCAAAGGAAAGTTGTATTGGTCGGGTAAAGCCGGGATTATGGAGTTGGATCCGTTGGCATACGACAGTAACAAAAACATTAGTACTAGTAATGCTGATGTTTTCGATAAATGTCGCTTCATTAATATTGATCACGAGCATCCAGATGAATCGGATTACTCTAGATCTAGACGCTTATGTGTGCGGCGAGGCTGTTTGTGCCTCACAGGTGTGGTCAATGGGAGTCGCGTTCATATTTGGGAGTTCAAGGGAGAGATTGAGGAAGGAAACTTTTGCTTGATATATAACGTTTCCTTGTCCCCAATGCTCGATGAATCTGGTCTTCGTCACTTGCATCGTGCTTCATCTAAACTGCTGCATTTGAACTCGTTTGATGAGGATATCGTGTATATAGAAGCGGATAAATGCATCTTCATTTGCAACATTCGTACAGAGACGGTTCTGGCTAAGCATGAATCGGATGATAAAAAAATCCGTGCGTTCCCGTTTGTCCTCCCACGGTGGCCCACACCACTTCCTAGCATAGCCACCATGCGTGCTTCCACAACAAGCAGTTTAAGTAAGCTCAGATTAGAAGTCCAGAACTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

440

Amino Acids

51.32

Weight (kDa)

8.54

Isoelectric Point (pI)

51.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 12 - 54 3.9e-07 F-box-like
b-prop_At3g26010-like PF24750 83 - 396 7.5e-11 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 123, 432
AccII CGCG 1 cut(s) 957
AciI CCGC 3 cut(s) 446, 913, 1127
AclI AACGTT 1 cut(s) 1017
AclWI GGATC 3 cut(s) 488, 773, 786
AcsI RAATTY 3 cut(s) 455, 517, 703
AfaI GTAC 5 cut(s) 208, 535, 657, 814, 1159
AfiI CCNNNNNNNGG 3 cut(s) 123, 203, 432
AgsI TTSAA 4 cut(s) 454, 733, 976, 1093
AhlI ACTAGT 2 cut(s) 536, 815
AjiI CACGTC 1 cut(s) 236
AjuI GAANNNNNNNTTGG 4 cut(s) 110, 142, 730, 762
AleI CACNNNNGTG 1 cut(s) 938
AluBI AGCT 3 cut(s) 265, 371, 1294
AluI AGCT 3 cut(s) 265, 371, 1294
Alw21I GWGCWC 2 cut(s) 135, 687
Alw26I GTCTC 2 cut(s) 623, 1157
AlwI GGATC 3 cut(s) 488, 773, 786
AoxI GGCC 2 cut(s) 708, 1232
ApeKI GCWGC 1 cut(s) 1084
ApoI RAATTY 3 cut(s) 455, 517, 703
AseI ATTAAT 2 cut(s) 36, 852
Asp700I GAANNNNTTC 1 cut(s) 70
AspS9I GGNCC 2 cut(s) 709, 1233
AsuC2I CCSGG 2 cut(s) 313, 763
AsuHPI GGTGA 3 cut(s) 230, 625, 647
AsuII TTCGAA 1 cut(s) 64
AxyI CCTNAGG 1 cut(s) 48
BamHI GGATCC 1 cut(s) 778
BauI CACGAG 1 cut(s) 863
BbsI GAAGAC 1 cut(s) 1043
Bbv12I GWGCWC 2 cut(s) 135, 687
BbvI GCAGC 1 cut(s) 1071
BccI CCATC 3 cut(s) 206, 317, 524
BclI TGATCA 1 cut(s) 859
BcnI CCSGG 2 cut(s) 313, 763
BcoDI GTCTC 2 cut(s) 623, 1157
BcuI ACTAGT 2 cut(s) 536, 815
BfaI CTAG 6 cut(s) 297, 537, 816, 891, 897, 1248
BglII AGATCT 1 cut(s) 893
BisI GCNGC 2 cut(s) 914, 1085
BlpI GCTNAGC 1 cut(s) 1176
BlsI GCNGC 2 cut(s) 915, 1086
BmcAI AGTACT 1 cut(s) 814
Bme1390I CCNGG 2 cut(s) 313, 763
BmgBI CACGTC 1 cut(s) 236
BmgT120I GGNCC 2 cut(s) 709, 1233
BmiI GGNNCC 2 cut(s) 80, 780
BmrFI CCNGG 2 cut(s) 313, 763
BmsI GCATC 4 cut(s) 16, 877, 1072, 1146
BpiI GAAGAC 1 cut(s) 1043
Bpu1102I GCTNAGC 1 cut(s) 1176
Bpu14I TTCGAA 1 cut(s) 64
BpuMI CCSGG 2 cut(s) 313, 763
BsaJI CCNNGG 2 cut(s) 311, 1226
Bsc4I CCNNNNNNNGG 3 cut(s) 123, 203, 432
Bse1I ACTGG 2 cut(s) 160, 637
Bse21I CCTNAGG 1 cut(s) 48
Bse3DI GCAATG 1 cut(s) 437
BseDI CCNNGG 2 cut(s) 311, 1226
BseGI GGATG 2 cut(s) 868, 1195
BseLI CCNNNNNNNGG 3 cut(s) 123, 203, 432
BseMI GCAATG 1 cut(s) 437
BseMII CTCAG 2 cut(s) 39, 1309
BseNI ACTGG 2 cut(s) 160, 637
BseRI GAGGAG 2 cut(s) 125, 481
BseXI GCAGC 1 cut(s) 1071
BseYI CCCAGC 1 cut(s) 711
BsgI GTGCAG 1 cut(s) 583
Bsh1236I CGCG 1 cut(s) 957
BshFI GGCC 2 cut(s) 710, 1234
BsiHKAI GWGCWC 2 cut(s) 135, 687
BsiSI CCGG 3 cut(s) 313, 716, 762
BslFI GGGAC 1 cut(s) 1012
BslI CCNNNNNNNGG 3 cut(s) 123, 203, 432
BsmAI GTCTC 2 cut(s) 623, 1157
BsmBI CGTCTC 1 cut(s) 1157
BsmFI GGGAC 1 cut(s) 1012
BsnI GGCC 2 cut(s) 710, 1234
Bsp119I TTCGAA 1 cut(s) 64
Bsp1286I GDGCHC 2 cut(s) 135, 687
Bsp143I GATC 5 cut(s) 40, 493, 778, 859, 893
Bsp1720I GCTNAGC 1 cut(s) 1176
BspACI CCGC 3 cut(s) 446, 913, 1127
BspANI GGCC 2 cut(s) 710, 1234
BspCNI CTCAG 2 cut(s) 40, 1308
BspFNI CGCG 1 cut(s) 957
BspLI GGNNCC 2 cut(s) 80, 780
BspPI GGATC 3 cut(s) 488, 773, 786
BspQI GCTCTTC 1 cut(s) 447
BspT104I TTCGAA 1 cut(s) 64
BsrDI GCAATG 1 cut(s) 437
BsrI ACTGG 2 cut(s) 160, 637
BssECI CCNNGG 2 cut(s) 311, 1226
BssMI GATC 5 cut(s) 40, 493, 778, 859, 893
BssSI CACGAG 1 cut(s) 863
Bst2BI CACGAG 1 cut(s) 863
Bst4CI ACNGT 5 cut(s) 211, 345, 797, 1168, 1230
Bst6I CTCTTC 1 cut(s) 447
BstBI TTCGAA 1 cut(s) 64
BstC8I GCNNGC 1 cut(s) 1266
BstDEI CTNAG 4 cut(s) 48, 367, 1176, 1295
BstDSI CCRYGG 1 cut(s) 1226
BstF5I GGATG 2 cut(s) 868, 1195
BstFNI CGCG 1 cut(s) 957
BstKTI GATC 5 cut(s) 43, 496, 781, 862, 896
BstMAI GTCTC 2 cut(s) 623, 1157
BstMBI GATC 5 cut(s) 40, 493, 778, 859, 893
BstMWI GCNNNNNNNGC 1 cut(s) 927
BstNSI RCATGY 1 cut(s) 109
BstSCI CCNGG 2 cut(s) 311, 761
BstUI CGCG 1 cut(s) 957
BstV1I GCAGC 1 cut(s) 1071
BstV2I GAAGAC 1 cut(s) 1043
BstX2I RGATCY 2 cut(s) 778, 893
BstYI RGATCY 2 cut(s) 778, 893
Bsu36I CCTNAGG 1 cut(s) 48
BsuRI GGCC 2 cut(s) 710, 1234
BtgI CCRYGG 1 cut(s) 1226
BtrI CACGTC 1 cut(s) 236
BtsCI GGATG 2 cut(s) 868, 1195
BtsIMutI CAGTG 1 cut(s) 341
Cac8I GCNNGC 1 cut(s) 1266
Cfr13I GGNCC 2 cut(s) 709, 1233
CseI GACGC 1 cut(s) 909
Csp6I GTAC 5 cut(s) 207, 534, 656, 813, 1158
CspCI CAANNNNNGTGG 2 cut(s) 230, 265
CviAII CATG 9 cut(s) 91, 106, 389, 427, 610, 660, 700, 1181, 1261
CviQI GTAC 5 cut(s) 207, 534, 656, 813, 1158
DdeI CTNAG 4 cut(s) 48, 367, 1176, 1295
DpnI GATC 5 cut(s) 42, 495, 780, 861, 895
DpnII GATC 5 cut(s) 40, 493, 778, 859, 893
Eam1104I CTCTTC 1 cut(s) 447
EarI CTCTTC 1 cut(s) 447
EciI GGCGGA 1 cut(s) 435
Eco32I GATATC 1 cut(s) 1111
Eco81I CCTNAGG 1 cut(s) 48
EcoRV GATATC 1 cut(s) 1111
EcoT22I ATGCAT 2 cut(s) 107, 1139
Esp3I CGTCTC 1 cut(s) 1157
FaeI CATG 9 cut(s) 94, 109, 392, 430, 613, 663, 703, 1184, 1264
FaqI GGGAC 1 cut(s) 1012
FatI CATG 9 cut(s) 90, 105, 388, 426, 609, 659, 699, 1180, 1260
FbaI TGATCA 1 cut(s) 859
Fnu4HI GCNGC 2 cut(s) 914, 1085
FokI GGATG 2 cut(s) 855, 1202
Fsp4HI GCNGC 2 cut(s) 914, 1085
FspBI CTAG 6 cut(s) 297, 537, 816, 891, 897, 1248
GluI GCNGC 2 cut(s) 914, 1085
GsaI CCCAGC 1 cut(s) 715
HaeIII GGCC 2 cut(s) 710, 1234
HapII CCGG 3 cut(s) 313, 716, 762
HgaI GACGC 1 cut(s) 909
Hin1II CATG 9 cut(s) 94, 109, 392, 430, 613, 663, 703, 1184, 1264
HinfI GANTC 7 cut(s) 66, 483, 692, 878, 952, 1043, 1184
HpaII CCGG 3 cut(s) 313, 716, 762
HphI GGTGA 3 cut(s) 230, 625, 647
Hpy188I TCNGA 5 cut(s) 322, 628, 883, 1189, 1298
Hpy188III TCNNGA 6 cut(s) 28, 863, 872, 891, 897, 1309
Hpy99I CGWCG 1 cut(s) 29
HpyAV CCTTC 3 cut(s) 317, 713, 986
HpyCH4III ACNGT 5 cut(s) 211, 345, 797, 1168, 1230
HpyCH4IV ACGT 4 cut(s) 24, 169, 235, 1017
HpyF10VI GCNNNNNNNGC 1 cut(s) 927
HpyF3I CTNAG 4 cut(s) 48, 367, 1176, 1295
HpySE526I ACGT 4 cut(s) 24, 169, 235, 1017
Hsp92II CATG 9 cut(s) 94, 109, 392, 430, 613, 663, 703, 1184, 1264
Ksp22I TGATCA 1 cut(s) 859
Kzo9I GATC 5 cut(s) 40, 493, 778, 859, 893
LguI GCTCTTC 1 cut(s) 447
LmnI GCTCC 4 cut(s) 4, 84, 138, 270
Lsp1109I GCAGC 1 cut(s) 1071
LweI GCATC 4 cut(s) 16, 877, 1072, 1146
MaeI CTAG 6 cut(s) 297, 537, 816, 891, 897, 1248
MaeII ACGT 4 cut(s) 24, 169, 235, 1017
MaeIII GTNAC 4 cut(s) 236, 545, 797, 1055
MalI GATC 5 cut(s) 42, 495, 780, 861, 895
MboI GATC 5 cut(s) 40, 493, 778, 859, 893
MboII GAAGA 5 cut(s) 434, 518, 615, 1043, 1132
MflI RGATCY 2 cut(s) 778, 893
MhlI GDGCHC 2 cut(s) 135, 687
MluCI AATT 6 cut(s) 182, 455, 517, 576, 602, 703
MlyI GAGTC 2 cut(s) 701, 961
MmeI TCCRAC 1 cut(s) 756
MnlI CCTC 8 cut(s) 43, 146, 459, 911, 941, 982, 1099, 1232
Mph1103I ATGCAT 2 cut(s) 107, 1139
MroXI GAANNNNTTC 1 cut(s) 70
MseI TTAA 5 cut(s) 36, 600, 605, 852, 1286
MslI CAYNNNNRTG 4 cut(s) 110, 614, 873, 938
MspI CCGG 3 cut(s) 313, 716, 762
MspR9I CCNGG 2 cut(s) 313, 763
MvnI CGCG 1 cut(s) 957
MwoI GCNNNNNNNGC 1 cut(s) 927
NciI CCSGG 2 cut(s) 313, 763
NdeII GATC 5 cut(s) 40, 493, 778, 859, 893
NlaIII CATG 9 cut(s) 94, 109, 392, 430, 613, 663, 703, 1184, 1264
NlaIV GGNNCC 2 cut(s) 80, 780
NmeAIII GCCGAG 1 cut(s) 55
NmuCI GTSAC 2 cut(s) 236, 1055
NsiI ATGCAT 2 cut(s) 107, 1139
NspI RCATGY 1 cut(s) 109
NspV TTCGAA 1 cut(s) 64
OliI CACNNNNGTG 1 cut(s) 938
PciSI GCTCTTC 1 cut(s) 447
PdmI GAANNNNTTC 1 cut(s) 70
PfeI GAWTC 5 cut(s) 66, 483, 878, 1043, 1184
PflMI CCANNNNNTGG 2 cut(s) 123, 432
PkrI GCNGC 2 cut(s) 915, 1086
PleI GAGTC 2 cut(s) 700, 960
PpsI GAGTC 2 cut(s) 700, 960
PshBI ATTAAT 2 cut(s) 36, 852
Psp1406I AACGTT 1 cut(s) 1017
PspFI CCCAGC 1 cut(s) 711
PspN4I GGNNCC 2 cut(s) 80, 780
PspPI GGNCC 2 cut(s) 709, 1233
PsuI RGATCY 2 cut(s) 778, 893
RsaI GTAC 5 cut(s) 208, 535, 657, 814, 1159
RsaNI GTAC 5 cut(s) 207, 534, 656, 813, 1158
RseI CAYNNNNRTG 4 cut(s) 110, 614, 873, 938
SapI GCTCTTC 1 cut(s) 447
SaqAI TTAA 5 cut(s) 36, 600, 605, 852, 1286
SatI GCNGC 2 cut(s) 914, 1085
Sau3AI GATC 5 cut(s) 40, 493, 778, 859, 893
Sau96I GGNCC 2 cut(s) 709, 1233
ScaI AGTACT 1 cut(s) 814
SchI GAGTC 2 cut(s) 701, 961
ScrFI CCNGG 2 cut(s) 313, 763
SduI GDGCHC 2 cut(s) 135, 687
SetI ASST 9 cut(s) 27, 172, 238, 267, 373, 563, 940, 1020, 1296
SfaNI GCATC 4 cut(s) 16, 877, 1072, 1146
SfuI TTCGAA 1 cut(s) 64
SmiMI CAYNNNNRTG 4 cut(s) 110, 614, 873, 938
SpeI ACTAGT 2 cut(s) 536, 815
Sse9I AATT 6 cut(s) 182, 455, 517, 576, 602, 703
SsiI CCGC 3 cut(s) 446, 913, 1127
SspI AATATT 2 cut(s) 34, 856
SspMI CTAG 6 cut(s) 297, 537, 816, 891, 897, 1248
StyD4I CCNGG 2 cut(s) 311, 761
TaaI ACNGT 5 cut(s) 211, 345, 797, 1168, 1230
TaiI ACGT 4 cut(s) 27, 172, 238, 1020
TaqI TCGA 5 cut(s) 64, 353, 459, 834, 1038
TasI AATT 6 cut(s) 182, 455, 517, 576, 602, 703
TatI WGTACW 1 cut(s) 812
TauI GCSGC 1 cut(s) 916
TfiI GAWTC 5 cut(s) 66, 483, 878, 1043, 1184
Tru1I TTAA 5 cut(s) 36, 600, 605, 852, 1286
Tru9I TTAA 5 cut(s) 36, 600, 605, 852, 1286
TscAI CASTG 1 cut(s) 348
TseFI GTSAC 2 cut(s) 236, 1055
TseI GCWGC 1 cut(s) 1084
Tsp45I GTSAC 2 cut(s) 236, 1055
TspGWI ACGGA 2 cut(s) 771, 1193
TspRI CASTG 1 cut(s) 348
Van91I CCANNNNNTGG 2 cut(s) 123, 432
VspI ATTAAT 2 cut(s) 36, 852
XapI RAATTY 3 cut(s) 455, 517, 703
XbaI TCTAGA 2 cut(s) 890, 896
XceI RCATGY 1 cut(s) 109
XmnI GAANNNNTTC 1 cut(s) 70
XspI CTAG 6 cut(s) 297, 537, 816, 891, 897, 1248
ZrmI AGTACT 1 cut(s) 814
Zsp2I ATGCAT 2 cut(s) 107, 1139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.