Rh2DG540900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0014632
Physical Location & Seq
Forward (+)
77004990 .. 77005709
720 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG540900.1

Sequence Viewer

Length: 720 bp
ATGGACATCTTTTTCTCTGAGACCGGTGAATGGAGAGAATTTGTTGTATTATGCCCGGAAAGAGCTATTGAATATTGCAGTATCGACATTACTGAGAAAAACTCTTACAATAATAGTGGAGGCCTTGTGTACAATGGAAAGTTGTATTGGTTAGGCGAAGGTGGCATCATGGAGTTGGATCCCTTGGAATTTGACAGTAATACTAGTTATAGTGATCTCATCAATCAGGGGTATTGTCGTTTCATTCAATTTCAAGTTTTCGGAAACCCTATCTTCGAAAAGATTCGCCTAAGTGTATGCCGAGGGTGTATGTGCCTCGTGTGGTTTCGGGATAGTGACTTTGCAATTTGCGAGTTGGAATTAGGAGATGATGATCAGTTGGATGGAGGCAAATGGTGTTTTACACTTACTACACGGCGGTGCATACCTCTTAACCACGATACTGGGAGGCCTTGGCTGCTATCTTTGGACTCATTCAATGAGGATTGCTTCTATGTGATCTCCAAATCTGGATACTTCGGCAAGGGCAACATGAGGACCGGAGTGATAAAGTACAATACAGTGAAGCGATGCCCTGGTCTTGGCTTTCGGGATTCATTTGGAGCCTTCCCATTTGTGCTCCCATGGTGGCCGACACCAGTTCCTAGAATTAAAAATGACCACAAACAACCAGTTCCTAGTGGTATTGACATTCTGTGCAGTTTGTTACTGGATCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

239

Amino Acids

27.38

Weight (kDa)

4.86

Isoelectric Point (pI)

27.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 418
AclWI GGATC 3 cut(s) 173, 186, 707
AcoI YGGCCR 1 cut(s) 629
AcsI RAATTY 2 cut(s) 38, 188
AfaI GTAC 2 cut(s) 131, 554
AfiI CCNNNNNNNGG 2 cut(s) 30, 581
AgeI ACCGGT 1 cut(s) 23
AgsI TTSAA 4 cut(s) 71, 248, 254, 478
AhlI ACTAGT 1 cut(s) 203
AjnI CCWGG 1 cut(s) 574
AjuI GAANNNNNNNTTGG 2 cut(s) 130, 162
AleI CACNNNNGTG 1 cut(s) 418
AluBI AGCT 1 cut(s) 65
AluI AGCT 1 cut(s) 65
Alw21I GWGCWC 1 cut(s) 621
Alw26I GTCTC 1 cut(s) 14
AlwI GGATC 3 cut(s) 173, 186, 707
AoxI GGCC 3 cut(s) 121, 449, 629
ApeKI GCWGC 1 cut(s) 457
ApoI RAATTY 2 cut(s) 38, 188
AsiGI ACCGGT 1 cut(s) 23
Asp700I GAANNNNTTC 1 cut(s) 282
AspS9I GGNCC 1 cut(s) 537
AsuC2I CCSGG 1 cut(s) 56
AsuHPI GGTGA 1 cut(s) 38
AsuII TTCGAA 1 cut(s) 276
AvaII GGWCC 1 cut(s) 537
BamHI GGATCC 2 cut(s) 178, 712
BauI CACGAG 1 cut(s) 317
Bbv12I GWGCWC 1 cut(s) 621
BbvI GCAGC 1 cut(s) 444
BccI CCATC 1 cut(s) 377
BceAI ACGGC 1 cut(s) 431
BciT130I CCWGG 1 cut(s) 576
BciVI GTATCC 1 cut(s) 506
BclI TGATCA 1 cut(s) 373
BcnI CCSGG 1 cut(s) 56
BcoDI GTCTC 1 cut(s) 14
BcuI ACTAGT 1 cut(s) 203
BfaI CTAG 3 cut(s) 204, 645, 678
BfuI GTATCC 1 cut(s) 506
BisI GCNGC 1 cut(s) 458
BlsI GCNGC 1 cut(s) 459
Bme1390I CCNGG 2 cut(s) 56, 576
Bme18I GGWCC 1 cut(s) 537
BmgT120I GGNCC 1 cut(s) 537
BmiI GGNNCC 3 cut(s) 180, 604, 714
BmrFI CCNGG 2 cut(s) 56, 576
BmrI ACTGGG 1 cut(s) 453
BmsI GCATC 2 cut(s) 174, 560
BmuI ACTGGG 1 cut(s) 453
BplI GAGNNNNNCTC 2 cut(s) 86, 118
Bpu14I TTCGAA 1 cut(s) 276
BpuMI CCSGG 1 cut(s) 56
BsaBI GATNNNNATC 1 cut(s) 372
BsaI GGTCTC 1 cut(s) 14
BsaJI CCNNGG 5 cut(s) 183, 301, 452, 574, 623
BsaWI WCCGGW 2 cut(s) 23, 539
BsaXI ACNNNNNCTCC 2 cut(s) 111, 141
Bsc4I CCNNNNNNNGG 2 cut(s) 30, 581
Bse118I RCCGGY 1 cut(s) 23
Bse1I ACTGG 4 cut(s) 448, 638, 671, 714
Bse8I GATNNNNATC 1 cut(s) 372
BseBI CCWGG 1 cut(s) 576
BseDI CCNNGG 5 cut(s) 183, 301, 452, 574, 623
BseGI GGATG 1 cut(s) 388
BseJI GATNNNNATC 1 cut(s) 372
BseLI CCNNNNNNNGG 2 cut(s) 30, 581
BseMII CTCAG 2 cut(s) 9, 84
BseNI ACTGG 4 cut(s) 448, 638, 671, 714
BseXI GCAGC 1 cut(s) 444
BsgI GTGCAG 1 cut(s) 718
BshFI GGCC 3 cut(s) 123, 451, 631
BshTI ACCGGT 1 cut(s) 23
BsiHKAI GWGCWC 1 cut(s) 621
BsiSI CCGG 3 cut(s) 24, 56, 540
BslI CCNNNNNNNGG 2 cut(s) 30, 581
BsmAI GTCTC 1 cut(s) 14
BsnI GGCC 3 cut(s) 123, 451, 631
Bso31I GGTCTC 1 cut(s) 14
Bsp119I TTCGAA 1 cut(s) 276
Bsp1286I GDGCHC 1 cut(s) 621
Bsp1407I TGTACA 1 cut(s) 129
Bsp143I GATC 5 cut(s) 178, 214, 373, 498, 712
Bsp19I CCATGG 1 cut(s) 623
BspACI CCGC 1 cut(s) 418
BspANI GGCC 3 cut(s) 123, 451, 631
BspCNI CTCAG 2 cut(s) 10, 85
BspLI GGNNCC 3 cut(s) 180, 604, 714
BspPI GGATC 3 cut(s) 173, 186, 707
BspT104I TTCGAA 1 cut(s) 276
BspTNI GGTCTC 1 cut(s) 14
BsrFI RCCGGY 1 cut(s) 23
BsrGI TGTACA 1 cut(s) 129
BsrI ACTGG 4 cut(s) 448, 638, 671, 714
BssAI RCCGGY 1 cut(s) 23
BssECI CCNNGG 5 cut(s) 183, 301, 452, 574, 623
BssMI GATC 5 cut(s) 178, 214, 373, 498, 712
BssSI CACGAG 1 cut(s) 317
BssT1I CCWWGG 3 cut(s) 183, 452, 623
Bst2BI CACGAG 1 cut(s) 317
Bst2UI CCWGG 1 cut(s) 576
Bst4CI ACNGT 2 cut(s) 197, 562
BstAUI TGTACA 1 cut(s) 129
BstBI TTCGAA 1 cut(s) 276
BstDEI CTNAG 3 cut(s) 18, 93, 290
BstDSI CCRYGG 1 cut(s) 623
BstF5I GGATG 1 cut(s) 388
BstKTI GATC 5 cut(s) 181, 217, 376, 501, 715
BstMAI GTCTC 1 cut(s) 14
BstMBI GATC 5 cut(s) 178, 214, 373, 498, 712
BstMWI GCNNNNNNNGC 2 cut(s) 162, 457
BstNI CCWGG 1 cut(s) 576
BstSCI CCNGG 2 cut(s) 54, 574
BstV1I GCAGC 1 cut(s) 444
BstX2I RGATCY 2 cut(s) 178, 712
BstXI CCANNNNNNTGG 1 cut(s) 443
BstYI RGATCY 2 cut(s) 178, 712
BsuI GTATCC 1 cut(s) 506
BsuRI GGCC 3 cut(s) 123, 451, 631
BtgI CCRYGG 1 cut(s) 623
BtgZI GCGATG 1 cut(s) 583
BtsCI GGATG 1 cut(s) 388
BtsIMutI CAGTG 1 cut(s) 567
Cfr10I RCCGGY 1 cut(s) 23
Cfr13I GGNCC 1 cut(s) 537
Csp6I GTAC 2 cut(s) 130, 553
CspAI ACCGGT 1 cut(s) 23
CspCI CAANNNNNGTGG 2 cut(s) 97, 132
CviAII CATG 3 cut(s) 169, 532, 624
CviJI RGCY 7 cut(s) 65, 123, 451, 457, 585, 605, 631
CviKI_1 RGCY 7 cut(s) 65, 123, 451, 457, 585, 605, 631
CviQI GTAC 2 cut(s) 130, 553
DdeI CTNAG 3 cut(s) 18, 93, 290
DpnI GATC 5 cut(s) 180, 216, 375, 500, 714
DpnII GATC 5 cut(s) 178, 214, 373, 498, 712
EaeI YGGCCR 1 cut(s) 629
Eco130I CCWWGG 3 cut(s) 183, 452, 623
Eco147I AGGCCT 2 cut(s) 123, 451
Eco31I GGTCTC 1 cut(s) 14
Eco47I GGWCC 1 cut(s) 537
EcoRII CCWGG 1 cut(s) 574
EcoT14I CCWWGG 3 cut(s) 183, 452, 623
ErhI CCWWGG 3 cut(s) 183, 452, 623
FaeI CATG 3 cut(s) 172, 535, 627
FatI CATG 3 cut(s) 168, 531, 623
FbaI TGATCA 1 cut(s) 373
Fnu4HI GCNGC 1 cut(s) 458
FokI GGATG 1 cut(s) 395
Fsp4HI GCNGC 1 cut(s) 458
FspBI CTAG 3 cut(s) 204, 645, 678
GluI GCNGC 1 cut(s) 458
HaeIII GGCC 3 cut(s) 123, 451, 631
HapII CCGG 3 cut(s) 24, 56, 540
Hin1II CATG 3 cut(s) 172, 535, 627
HinfI GANTC 3 cut(s) 283, 470, 593
HpaII CCGG 3 cut(s) 24, 56, 540
HphI GGTGA 1 cut(s) 38
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 2 cut(s) 19, 263
Hpy188III TCNNGA 3 cut(s) 329, 510, 590
Hpy8I GTNNAC 1 cut(s) 130
HpyAV CCTTC 2 cut(s) 152, 616
HpyCH4III ACNGT 2 cut(s) 197, 562
HpyCH4V TGCA 4 cut(s) 78, 344, 423, 699
HpyF10VI GCNNNNNNNGC 2 cut(s) 162, 457
HpyF3I CTNAG 3 cut(s) 18, 93, 290
Hsp92II CATG 3 cut(s) 172, 535, 627
Ksp22I TGATCA 1 cut(s) 373
Kzo9I GATC 5 cut(s) 178, 214, 373, 498, 712
LmnI GCTCC 2 cut(s) 602, 624
Lsp1109I GCAGC 1 cut(s) 444
LweI GCATC 2 cut(s) 174, 560
MaeI CTAG 3 cut(s) 204, 645, 678
MaeIII GTNAC 2 cut(s) 335, 705
MalI GATC 5 cut(s) 180, 216, 375, 500, 714
MboI GATC 5 cut(s) 178, 214, 373, 498, 712
MboII GAAGA 1 cut(s) 265
MflI RGATCY 2 cut(s) 178, 712
MhlI GDGCHC 1 cut(s) 621
MluCI AATT 6 cut(s) 38, 188, 248, 345, 359, 648
MlyI GAGTC 1 cut(s) 464
MmeI TCCRAC 3 cut(s) 156, 336, 360
MnlI CCTC 8 cut(s) 113, 296, 326, 380, 438, 441, 475, 528
MroXI GAANNNNTTC 1 cut(s) 282
MseI TTAA 2 cut(s) 432, 651
MslI CAYNNNNRTG 1 cut(s) 418
MspI CCGG 3 cut(s) 24, 56, 540
MspR9I CCNGG 2 cut(s) 56, 576
MvaI CCWGG 1 cut(s) 576
MwoI GCNNNNNNNGC 2 cut(s) 162, 457
NciI CCSGG 1 cut(s) 56
NcoI CCATGG 1 cut(s) 623
NdeII GATC 5 cut(s) 178, 214, 373, 498, 712
NlaIII CATG 3 cut(s) 172, 535, 627
NlaIV GGNNCC 3 cut(s) 180, 604, 714
NmeAIII GCCGAG 1 cut(s) 326
NmuCI GTSAC 1 cut(s) 335
NspV TTCGAA 1 cut(s) 276
OliI CACNNNNGTG 1 cut(s) 418
PceI AGGCCT 2 cut(s) 123, 451
PdmI GAANNNNTTC 1 cut(s) 282
PfeI GAWTC 2 cut(s) 283, 593
PinAI ACCGGT 1 cut(s) 23
PkrI GCNGC 1 cut(s) 459
PleI GAGTC 1 cut(s) 464
PpsI GAGTC 1 cut(s) 464
Psp6I CCWGG 1 cut(s) 574
PspGI CCWGG 1 cut(s) 574
PspN4I GGNNCC 3 cut(s) 180, 604, 714
PspPI GGNCC 1 cut(s) 537
PsuI RGATCY 2 cut(s) 178, 712
RsaI GTAC 2 cut(s) 131, 554
RsaNI GTAC 2 cut(s) 130, 553
RseI CAYNNNNRTG 1 cut(s) 418
SaqAI TTAA 2 cut(s) 432, 651
SatI GCNGC 1 cut(s) 458
Sau3AI GATC 5 cut(s) 178, 214, 373, 498, 712
Sau96I GGNCC 1 cut(s) 537
SchI GAGTC 1 cut(s) 464
ScrFI CCNGG 2 cut(s) 56, 576
SduI GDGCHC 1 cut(s) 621
SetI ASST 3 cut(s) 67, 163, 430
SfaNI GCATC 2 cut(s) 174, 560
SfuI TTCGAA 1 cut(s) 276
SinI GGWCC 1 cut(s) 537
SmiMI CAYNNNNRTG 1 cut(s) 418
SpeI ACTAGT 1 cut(s) 203
Sse9I AATT 6 cut(s) 38, 188, 248, 345, 359, 648
SseBI AGGCCT 2 cut(s) 123, 451
SsiI CCGC 1 cut(s) 418
SspI AATATT 1 cut(s) 74
SspMI CTAG 3 cut(s) 204, 645, 678
StuI AGGCCT 2 cut(s) 123, 451
StyD4I CCNGG 2 cut(s) 54, 574
StyI CCWWGG 3 cut(s) 183, 452, 623
TaaI ACNGT 2 cut(s) 197, 562
TaqI TCGA 2 cut(s) 84, 276
TasI AATT 6 cut(s) 38, 188, 248, 345, 359, 648
TatI WGTACW 2 cut(s) 129, 552
TfiI GAWTC 2 cut(s) 283, 593
Tru1I TTAA 2 cut(s) 432, 651
Tru9I TTAA 2 cut(s) 432, 651
TscAI CASTG 1 cut(s) 567
TseFI GTSAC 1 cut(s) 335
TseI GCWGC 1 cut(s) 457
Tsp45I GTSAC 1 cut(s) 335
TspDTI ATGAA 2 cut(s) 232, 585
TspRI CASTG 1 cut(s) 567
VpaK11BI GGWCC 1 cut(s) 537
XapI RAATTY 2 cut(s) 38, 188
XmnI GAANNNNTTC 1 cut(s) 282
XspI CTAG 3 cut(s) 204, 645, 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.