MD05G1343000.v1.1
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0015787
Physical Location & Seq
Reverse (-)
46333471 .. 46335301
1831 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1343000.v1.1.491

Sequence Viewer

Length: 1176 bp
ATGACTTGCTTCTCGTGTTGCAAGGTGGACGAGGAGCCGGAAGAATCTGTGAGGAAGGCCAGAATAAGAAGGCAATACTCGAGTGGTTTGAAGAGATTGACCTCACTTATAGCAACTTTTTCGGTTAAGTCAGGTAGCAACGAATGCAGGAATTTACAGAAGGAGATACTCAAGACGGGACACGCGAAAAAAGTTGCTCAGCTTTTTACGTACGAGGAACTAGCTGCTGCAACTGATAACTTCAATCCTGATTCTCTGCTGGGAGAAGGTGGATTTGGGAAGGTGTACAGAGGTTACTTGGACAGCATTAACCAAATTGTGGCAGTGAAGAAACTTGACAGGAAGGGATTGCAAGGGAGCAGAGAGTTTTGTTCTGAAGTTATCATGTTGAGTTTGGTTCAACACGCGAACCTCGTCGATCTTATTGGCTATTGTGCAGAAGGAGACCAGAGAATGTTAGTGTATGAATTCATGGTCAATGGATCTTTGGAGAATCACCTTCTAGATGTATATTCGAATATGGAGCCTCTTGATTGGTATACGAGGATGAAAATAGCTGAAGGAGCAGCTAAAGGGCTCGAGTACCTTCATGAGACTGCCAGTCCGCCAGTGATTTACCGTGACTTCAAAGCTTCAAACATTATACTAGACGAAAAATTCTGTCCTAAGCTTTCTGATTTTGGACTTGCTAAGTTAGGTCCAACCGGAGACAAGGACCATGTGACTACTAGAGTGATGGGGACTTATGGCTATTGTGCACCAGAGTATGCTTCAACAGGACAGCTGACAACTAGGTCTGATGTCTACAGCTTTGGCGTTGTGCTTCTCGAGTTGATCACAGGACGGAGAGCTATTGATGAGCGCAGACCGGTTGGACAGCAGATCCTAGTCAGTTGGGCAAAGCCAATGCTTAAGGACAGAAAGAGATACGCGTCGATGGCTGACCCGTTGCTTCGAGGCAGATTTCCGGCAAAGGGTCTGAACCAAGCTGTTGCAATTGCTGCAATGTGTCTGAATGAGGAAGCTGATTGCCGGCCTTCGATGGGTGACGTTGTGACCGCTCTCGGGCACCTATCGTTGCAAGAATACGATGGAGAAGGTGCCAAGGGTGCATCGAGAAACAGAGGCCGGCACGTTGAGTCAATAAGAAGAGCAAGGGAAGACAAAGAGCTATAG

Protein Analysis

392

Amino Acids

43.59

Weight (kDa)

8.22

Isoelectric Point (pI)

32.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 84 - 350 1.2e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 85 - 354 1.2e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015787)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61860
fragaria_vesca FvH4_3g02440
malus_domestica MD05G1343000.v1.1 MD10G1318000.v1.1
prunus_persica Prupe.4G023800_v2.0.a1
pyrus_communis pycom05g31250
rosa_chinensis RchiOBHm_Chr5g0003691
rosa_laevigata RLG00000031166
rosa_multiflora Rmu_sc0000500.1_g000001
rosa_rugosa Rorug04G0405900
rosa_samantha Rh5AG032700 Rh5BG031300 Rh5CG035400 Rh5DG031900
rosa_wichuraiana Rw5G003030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 793
AccB1I GGYRCC 2 cut(s) 1068, 1100
AccB7I CCANNNNNTGG 1 cut(s) 319
AccBSI CCGCTC 1 cut(s) 1061
AccI GTMKAC 2 cut(s) 539, 804
AccII CGCG 3 cut(s) 185, 407, 932
AciI CCGC 2 cut(s) 605, 1059
AclWI GGATC 2 cut(s) 490, 877
AcsI RAATTY 3 cut(s) 151, 467, 656
AcuI CTGAAG 2 cut(s) 396, 579
AfaI GTAC 3 cut(s) 212, 287, 584
AfiI CCNNNNNNNGG 4 cut(s) 319, 974, 1043, 1065
AflII CTTAAG 1 cut(s) 911
AflIII ACRYGT 1 cut(s) 930
AgeI ACCGGT 1 cut(s) 868
AgsI TTSAA 6 cut(s) 91, 244, 401, 628, 636, 774
AhdI GACNNNNNGTC 1 cut(s) 600
AjuI GAANNNNNNNTTGG 2 cut(s) 258, 290
Alw21I GWGCWC 1 cut(s) 760
Alw26I GTCTC 3 cut(s) 438, 587, 702
Alw44I GTGCAC 1 cut(s) 756
AlwI GGATC 2 cut(s) 490, 877
Ama87I CYCGRG 4 cut(s) 79, 578, 827, 1064
AoxI GGCC 3 cut(s) 57, 1034, 1126
ApaLI GTGCAC 1 cut(s) 756
ApeKI GCWGC 4 cut(s) 224, 227, 566, 1001
ApoI RAATTY 3 cut(s) 151, 467, 656
AsiGI ACCGGT 1 cut(s) 868
AspLEI GCGC 1 cut(s) 864
AspS9I GGNCC 2 cut(s) 698, 715
AsuHPI GGTGA 2 cut(s) 488, 1058
AsuII TTCGAA 1 cut(s) 515
AvaI CYCGRG 4 cut(s) 79, 578, 827, 1064
AvaII GGWCC 2 cut(s) 698, 715
BaeGI GKGCMC 2 cut(s) 760, 1071
BanI GGYRCC 2 cut(s) 1068, 1100
BanII GRGCYC 1 cut(s) 579
BauI CACGAG 1 cut(s) 13
BbsI GAAGAC 1 cut(s) 1167
Bbv12I GWGCWC 1 cut(s) 760
BbvI GCAGC 4 cut(s) 211, 214, 578, 988
BccI CCATC 4 cut(s) 730, 931, 1036, 1085
BcgI CGANNNNNNTGC 2 cut(s) 102, 136
BclI TGATCA 1 cut(s) 834
BcoDI GTCTC 3 cut(s) 438, 587, 702
BfaI CTAG 6 cut(s) 221, 503, 647, 729, 792, 887
BfmI CTRYAG 2 cut(s) 805, 1172
BfrI CTTAAG 1 cut(s) 911
BisI GCNGC 4 cut(s) 225, 228, 567, 1002
BlpI GCTNAGC 1 cut(s) 198
BlsI GCNGC 4 cut(s) 226, 229, 568, 1003
Bme18I GGWCC 2 cut(s) 698, 715
BmeRI GACNNNNNGTC 1 cut(s) 600
BmeT110I CYCGRG 4 cut(s) 79, 578, 827, 1064
BmgT120I GGNCC 2 cut(s) 698, 715
BmiI GGNNCC 4 cut(s) 36, 525, 1070, 1102
BmsI GCATC 1 cut(s) 1121
BpiI GAAGAC 1 cut(s) 1167
Bpu10I CCTNAGC 1 cut(s) 666
Bpu1102I GCTNAGC 1 cut(s) 198
Bpu14I TTCGAA 1 cut(s) 515
BpuEI CTTGAG 1 cut(s) 155
BsaAI YACGTR 1 cut(s) 210
BsaI GGTCTC 1 cut(s) 438
BsaJI CCNNGG 1 cut(s) 1104
BsaWI WCCGGW 2 cut(s) 704, 868
Bsc4I CCNNNNNNNGG 4 cut(s) 319, 974, 1043, 1065
Bse118I RCCGGY 3 cut(s) 868, 1032, 1128
Bse1I ACTGG 2 cut(s) 600, 608
Bse3DI GCAATG 1 cut(s) 1011
BseDI CCNNGG 1 cut(s) 1104
BseGI GGATG 1 cut(s) 552
BseLI CCNNNNNNNGG 4 cut(s) 319, 974, 1043, 1065
BseMI GCAATG 1 cut(s) 1011
BseMII CTCAG 1 cut(s) 212
BseNI ACTGG 2 cut(s) 600, 608
BseRI GAGGAG 1 cut(s) 47
BseSI GKGCMC 2 cut(s) 760, 1071
BseXI GCAGC 4 cut(s) 211, 214, 578, 988
BseYI CCCAGC 1 cut(s) 259
BsgI GTGCAG 1 cut(s) 456
Bsh1236I CGCG 3 cut(s) 185, 407, 932
BshFI GGCC 3 cut(s) 59, 1036, 1128
BshNI GGYRCC 2 cut(s) 1068, 1100
BshTI ACCGGT 1 cut(s) 868
BsiHKAI GWGCWC 1 cut(s) 760
BsiHKCI CYCGRG 4 cut(s) 79, 578, 827, 1064
BsiSI CCGG 6 cut(s) 38, 705, 869, 968, 1033, 1129
BsiWI CGTACG 1 cut(s) 210
BslFI GGGAC 2 cut(s) 192, 754
BslI CCNNNNNNNGG 4 cut(s) 319, 974, 1043, 1065
BsmAI GTCTC 3 cut(s) 438, 587, 702
BsmFI GGGAC 2 cut(s) 192, 754
BsmI GAATGC 1 cut(s) 149
BsnI GGCC 3 cut(s) 59, 1036, 1128
Bso31I GGTCTC 1 cut(s) 438
BsoBI CYCGRG 4 cut(s) 79, 578, 827, 1064
Bsp119I TTCGAA 1 cut(s) 515
Bsp1286I GDGCHC 3 cut(s) 579, 760, 1071
Bsp1407I TGTACA 1 cut(s) 285
Bsp143I GATC 4 cut(s) 418, 482, 834, 882
Bsp1720I GCTNAGC 1 cut(s) 198
BspACI CCGC 2 cut(s) 605, 1059
BspANI GGCC 3 cut(s) 59, 1036, 1128
BspCNI CTCAG 1 cut(s) 211
BspFNI CGCG 3 cut(s) 185, 407, 932
BspHI TCATGA 1 cut(s) 589
BspLI GGNNCC 4 cut(s) 36, 525, 1070, 1102
BspPI GGATC 2 cut(s) 490, 877
BspQI GCTCTTC 1 cut(s) 1144
BspT104I TTCGAA 1 cut(s) 515
BspT107I GGYRCC 2 cut(s) 1068, 1100
BspTI CTTAAG 1 cut(s) 911
BspTNI GGTCTC 1 cut(s) 438
BsrBI CCGCTC 1 cut(s) 1061
BsrDI GCAATG 1 cut(s) 1011
BsrFI RCCGGY 3 cut(s) 868, 1032, 1128
BsrGI TGTACA 1 cut(s) 285
BsrI ACTGG 2 cut(s) 600, 608
BssAI RCCGGY 3 cut(s) 868, 1032, 1128
BssECI CCNNGG 1 cut(s) 1104
BssMI GATC 4 cut(s) 418, 482, 834, 882
BssNAI GTATAC 1 cut(s) 540
BssSI CACGAG 1 cut(s) 13
BssT1I CCWWGG 1 cut(s) 1104
Bst1107I GTATAC 1 cut(s) 540
Bst2BI CACGAG 1 cut(s) 13
Bst4CI ACNGT 1 cut(s) 620
Bst6I CTCTTC 2 cut(s) 86, 1144
BstAFI CTTAAG 1 cut(s) 911
BstAPI GCANNNNNTGC 2 cut(s) 144, 1001
BstAUI TGTACA 1 cut(s) 285
BstBAI YACGTR 1 cut(s) 210
BstBI TTCGAA 1 cut(s) 515
BstC8I GCNNGC 2 cut(s) 1034, 1130
BstDEI CTNAG 3 cut(s) 198, 666, 690
BstF5I GGATG 1 cut(s) 552
BstFNI CGCG 3 cut(s) 185, 407, 932
BstHHI GCGC 1 cut(s) 864
BstKTI GATC 4 cut(s) 421, 485, 837, 885
BstMAI GTCTC 3 cut(s) 438, 587, 702
BstMBI GATC 4 cut(s) 418, 482, 834, 882
BstMWI GCNNNNNNNGC 5 cut(s) 144, 563, 938, 1001, 1109
BstSFI CTRYAG 2 cut(s) 805, 1172
BstSLI GKGCMC 2 cut(s) 760, 1071
BstSNI TACGTA 1 cut(s) 210
BstUI CGCG 3 cut(s) 185, 407, 932
BstV1I GCAGC 4 cut(s) 211, 214, 578, 988
BstV2I GAAGAC 1 cut(s) 1167
BstX2I RGATCY 2 cut(s) 482, 882
BstYI RGATCY 2 cut(s) 482, 882
BstZ17I GTATAC 1 cut(s) 540
BsuRI GGCC 3 cut(s) 59, 1036, 1128
BtsCI GGATG 1 cut(s) 552
BtsI GCAGTG 1 cut(s) 330
BtsIMutI CAGTG 2 cut(s) 330, 615
Cac8I GCNNGC 2 cut(s) 1034, 1130
CciI TCATGA 1 cut(s) 589
CfoI GCGC 1 cut(s) 864
Cfr10I RCCGGY 3 cut(s) 868, 1032, 1128
Cfr13I GGNCC 2 cut(s) 698, 715
CseI GACGC 1 cut(s) 921
Csp6I GTAC 3 cut(s) 211, 286, 583
CspAI ACCGGT 1 cut(s) 868
CviAII CATG 4 cut(s) 385, 472, 590, 719
CviQI GTAC 3 cut(s) 211, 286, 583
DdeI CTNAG 3 cut(s) 198, 666, 690
DpnI GATC 4 cut(s) 420, 484, 836, 884
DpnII GATC 4 cut(s) 418, 482, 834, 882
DrdI GACNNNNNNGTC 1 cut(s) 793
DriI GACNNNNNGTC 1 cut(s) 600
DseDI GACNNNNNNGTC 1 cut(s) 793
Eam1104I CTCTTC 2 cut(s) 86, 1144
Eam1105I GACNNNNNGTC 1 cut(s) 600
EarI CTCTTC 2 cut(s) 86, 1144
EciI GGCGGA 1 cut(s) 594
Eco105I TACGTA 1 cut(s) 210
Eco130I CCWWGG 1 cut(s) 1104
Eco24I GRGCYC 1 cut(s) 579
Eco31I GGTCTC 1 cut(s) 438
Eco47I GGWCC 2 cut(s) 698, 715
Eco57I CTGAAG 2 cut(s) 396, 579
Eco88I CYCGRG 4 cut(s) 79, 578, 827, 1064
EcoRI GAATTC 1 cut(s) 467
EcoT14I CCWWGG 1 cut(s) 1104
EcoT38I GRGCYC 1 cut(s) 579
ErhI CCWWGG 1 cut(s) 1104
FaeI CATG 4 cut(s) 388, 475, 593, 722
FaqI GGGAC 2 cut(s) 192, 754
FatI CATG 4 cut(s) 384, 471, 589, 718
FbaI TGATCA 1 cut(s) 834
FblI GTMKAC 2 cut(s) 539, 804
Fnu4HI GCNGC 4 cut(s) 225, 228, 567, 1002
FokI GGATG 1 cut(s) 559
FriOI GRGCYC 1 cut(s) 579
Fsp4HI GCNGC 4 cut(s) 225, 228, 567, 1002
FspBI CTAG 6 cut(s) 221, 503, 647, 729, 792, 887
GlaI GCGC 1 cut(s) 863
GluI GCNGC 4 cut(s) 225, 228, 567, 1002
GsaI CCCAGC 1 cut(s) 263
HaeIII GGCC 3 cut(s) 59, 1036, 1128
HapII CCGG 6 cut(s) 38, 705, 869, 968, 1033, 1129
HgaI GACGC 1 cut(s) 921
HhaI GCGC 1 cut(s) 864
Hin1II CATG 4 cut(s) 388, 475, 593, 722
Hin6I GCGC 1 cut(s) 862
HinP1I GCGC 1 cut(s) 862
HindIII AAGCTT 2 cut(s) 630, 668
HinfI GANTC 4 cut(s) 44, 251, 493, 1139
HpaII CCGG 6 cut(s) 38, 705, 869, 968, 1033, 1129
HphI GGTGA 2 cut(s) 488, 1058
Hpy166II GTNNAC 5 cut(s) 28, 286, 540, 758, 805
Hpy188I TCNGA 5 cut(s) 376, 676, 799, 981, 1014
Hpy188III TCNNGA 7 cut(s) 172, 248, 503, 530, 590, 827, 1116
Hpy8I GTNNAC 5 cut(s) 28, 286, 540, 758, 805
Hpy99I CGWCG 2 cut(s) 419, 937
HpyCH4III ACNGT 1 cut(s) 620
HpyCH4IV ACGT 3 cut(s) 209, 1050, 1134
HpyF10VI GCNNNNNNNGC 5 cut(s) 144, 563, 938, 1001, 1109
HpyF3I CTNAG 3 cut(s) 198, 666, 690
HpySE526I ACGT 3 cut(s) 209, 1050, 1134
Hsp92II CATG 4 cut(s) 388, 475, 593, 722
HspAI GCGC 1 cut(s) 862
KroI GCCGGC 2 cut(s) 1032, 1128
KroNI GCCGGC 2 cut(s) 1034, 1130
Ksp22I TGATCA 1 cut(s) 834
Kzo9I GATC 4 cut(s) 418, 482, 834, 882
LguI GCTCTTC 1 cut(s) 1144
LmnI GCTCC 4 cut(s) 34, 357, 523, 563
Lsp1109I GCAGC 4 cut(s) 211, 214, 578, 988
LweI GCATC 1 cut(s) 1121
MaeI CTAG 6 cut(s) 221, 503, 647, 729, 792, 887
MaeII ACGT 3 cut(s) 209, 1050, 1134
MaeIII GTNAC 5 cut(s) 293, 620, 721, 1046, 1054
MalI GATC 4 cut(s) 420, 484, 836, 884
MbiI CCGCTC 1 cut(s) 1061
MboI GATC 4 cut(s) 418, 482, 834, 882
MboII GAAGA 5 cut(s) 53, 103, 340, 1161, 1172
MfeI CAATTG 1 cut(s) 996
MflI RGATCY 2 cut(s) 482, 882
MhlI GDGCHC 3 cut(s) 579, 760, 1071
MluCI AATT 5 cut(s) 151, 315, 467, 656, 996
MluI ACGCGT 1 cut(s) 930
MlyI GAGTC 1 cut(s) 1148
MmeI TCCRAC 2 cut(s) 725, 853
MroNI GCCGGC 2 cut(s) 1032, 1128
MseI TTAA 3 cut(s) 126, 309, 912
MspA1I CMGCKG 1 cut(s) 784
MspCI CTTAAG 1 cut(s) 911
MspI CCGG 6 cut(s) 38, 705, 869, 968, 1033, 1129
MunI CAATTG 1 cut(s) 996
Mva1269I GAATGC 1 cut(s) 149
MvnI CGCG 3 cut(s) 185, 407, 932
MwoI GCNNNNNNNGC 5 cut(s) 144, 563, 938, 1001, 1109
NaeI GCCGGC 2 cut(s) 1034, 1130
NdeII GATC 4 cut(s) 418, 482, 834, 882
NgoMIV GCCGGC 2 cut(s) 1032, 1128
NlaIII CATG 4 cut(s) 388, 475, 593, 722
NlaIV GGNNCC 4 cut(s) 36, 525, 1070, 1102
NmuCI GTSAC 4 cut(s) 620, 721, 1046, 1054
NspV TTCGAA 1 cut(s) 515
PaeR7I CTCGAG 3 cut(s) 79, 578, 827
PagI TCATGA 1 cut(s) 589
PciSI GCTCTTC 1 cut(s) 1144
PcsI WCGNNNNNNNCGW 2 cut(s) 182, 411
PctI GAATGC 1 cut(s) 149
PdiI GCCGGC 2 cut(s) 1034, 1130
PfeI GAWTC 3 cut(s) 44, 251, 493
Pfl23II CGTACG 1 cut(s) 210
PflMI CCANNNNNTGG 1 cut(s) 319
PinAI ACCGGT 1 cut(s) 868
PkrI GCNGC 4 cut(s) 226, 229, 568, 1003
PleI GAGTC 1 cut(s) 1147
PpsI GAGTC 1 cut(s) 1147
Ppu21I YACGTR 1 cut(s) 210
PspFI CCCAGC 1 cut(s) 259
PspLI CGTACG 1 cut(s) 210
PspN4I GGNNCC 4 cut(s) 36, 525, 1070, 1102
PspPI GGNCC 2 cut(s) 698, 715
PspXI VCTCGAGB 2 cut(s) 79, 578
PsuI RGATCY 2 cut(s) 482, 882
PvuII CAGCTG 1 cut(s) 784
RsaI GTAC 3 cut(s) 212, 287, 584
RsaNI GTAC 3 cut(s) 211, 286, 583
SapI GCTCTTC 1 cut(s) 1144
SaqAI TTAA 3 cut(s) 126, 309, 912
SatI GCNGC 4 cut(s) 225, 228, 567, 1002
Sau3AI GATC 4 cut(s) 418, 482, 834, 882
Sau96I GGNCC 2 cut(s) 698, 715
SchI GAGTC 1 cut(s) 1148
SduI GDGCHC 3 cut(s) 579, 760, 1071
SfaNI GCATC 1 cut(s) 1121
SfcI CTRYAG 2 cut(s) 805, 1172
Sfr274I CTCGAG 3 cut(s) 79, 578, 827
SfuI TTCGAA 1 cut(s) 515
SinI GGWCC 2 cut(s) 698, 715
SlaI CTCGAG 3 cut(s) 79, 578, 827
SmlI CTYRAG 5 cut(s) 79, 170, 578, 827, 911
SmoI CTYRAG 5 cut(s) 79, 170, 578, 827, 911
SnaBI TACGTA 1 cut(s) 210
Sse9I AATT 5 cut(s) 151, 315, 467, 656, 996
SsiI CCGC 2 cut(s) 605, 1059
SspMI CTAG 6 cut(s) 221, 503, 647, 729, 792, 887
StyI CCWWGG 1 cut(s) 1104
TaaI ACNGT 1 cut(s) 620
TaiI ACGT 3 cut(s) 212, 1053, 1137
TaqI TCGA 9 cut(s) 80, 417, 515, 579, 828, 935, 955, 1040, 1115
TasI AATT 5 cut(s) 151, 315, 467, 656, 996
TatI WGTACW 1 cut(s) 285
TfiI GAWTC 3 cut(s) 44, 251, 493
Tru1I TTAA 3 cut(s) 126, 309, 912
Tru9I TTAA 3 cut(s) 126, 309, 912
TscAI CASTG 2 cut(s) 330, 615
TseFI GTSAC 4 cut(s) 620, 721, 1046, 1054
TseI GCWGC 4 cut(s) 224, 227, 566, 1001
Tsp45I GTSAC 4 cut(s) 620, 721, 1046, 1054
TspDTI ATGAA 4 cut(s) 460, 480, 563, 578
TspGWI ACGGA 1 cut(s) 859
TspRI CASTG 2 cut(s) 330, 615
Van91I CCANNNNNTGG 1 cut(s) 319
Vha464I CTTAAG 1 cut(s) 911
VneI GTGCAC 1 cut(s) 756
VpaK11BI GGWCC 2 cut(s) 698, 715
XapI RAATTY 3 cut(s) 151, 467, 656
XbaI TCTAGA 1 cut(s) 502
XhoI CTCGAG 3 cut(s) 79, 578, 827
XmiI GTMKAC 2 cut(s) 539, 804
XspI CTAG 6 cut(s) 221, 503, 647, 729, 792, 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.