Rh5AG032700
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0015787
Physical Location & Seq
Forward (+)
2245691 .. 2247407
1717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG032700.1

Sequence Viewer

Length: 969 bp
ATGCGCTGCTTCTCTTTTTGCAAGCCGGTGGAGGATGATCCTCCGAAATTTGTAGTCAGAAAGACAGGCCGAAGGCCATATTCTAATACTGCCACGACATTGGACTCCTTTTTGGCAACGCTCTCCCTTAAGTCAGGTAGCAGCAGATGCAGGAATTTGCGCAATCAGATACTCAAGGTGGGAAGTGCAAAGCAATCTGCTCGGCTTTTTACGTTCAATGAGCTTCATGCTGCAACTGAGGGCTTCAAGGCTGCTTGTTTGCTTGGAGAAGGAGGATTTGGGAAGGTGTACAAAGGGTACATAGATAGCATCAATTCTAAACAGGCTGTGGCGGTTAAGAAACTTAACCGGAAAGGAATGCAAGGAAGTAGAGAGTTTTGTGCTGAAGTTATCATGTTGAGTCTGTTTCAACACCCAAACCTTGTCAGTCTAGTAGGGTACTGTGCGGAAGGAGAGCAGAGAGTTTTAGTGTATGAATATATGGCCAGTGGATCTTTGGAGGATCACCTTCTAGACATAGGTCCGAATCAGCAGCCTCTTGATTGGTACACTAGGATGACAATAGCTGAAGGAGCAGCTAAAGGACTTGATTTCGGGGTTGTCTTTCTTGAGTTAATCACCGGAAGAAGAGCTGTTGATGAGACAAAACCAGAGGAAGAGCAGATCCTAGTCAGTTGGGCACAACCACTGTTGAAGGATAGAAGTAAATTTTCATTAATTGCTGATCCGTTGCTTAGGGGAAACTTTCCGGTGAAGGGTCTGCATCAAGCTCTTGCAATTGCAGCAATGTGTCTCAATGAGGAAGCTGATTGCCGACCTGTTATGTCTGATGTTGTACTTGCTCTTGAGCATTTATCCAAGCCAAGAAATGAAGTTGTCATTGATGATGAATCAAAACACAGCCCTGTGCAGCTGACCAATGTCCAATCTCTAAAACGAAGGAGTTCCAAAGCAGAGCTAGATCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

322

Amino Acids

35.74

Weight (kDa)

8.54

Isoelectric Point (pI)

38.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 86 - 198 1.5e-20 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 87 - 176 8.7e-14 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015787)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61860
fragaria_vesca FvH4_3g02440
malus_domestica MD05G1343000.v1.1 MD10G1318000.v1.1
prunus_persica Prupe.4G023800_v2.0.a1
pyrus_communis pycom05g31250
rosa_chinensis RchiOBHm_Chr5g0003691
rosa_laevigata RLG00000031166
rosa_multiflora Rmu_sc0000500.1_g000001
rosa_rugosa Rorug04G0405900
rosa_samantha Rh5AG032700 Rh5BG031300 Rh5CG035400 Rh5DG031900
rosa_wichuraiana Rw5G003030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 161
AciI CCGC 2 cut(s) 332, 446
AclWI GGATC 5 cut(s) 32, 499, 510, 658, 719
AcoI YGGCCR 1 cut(s) 483
AcsI RAATTY 3 cut(s) 47, 154, 707
AcuI CTGAAG 2 cut(s) 405, 588
AfaI GTAC 5 cut(s) 290, 299, 440, 548, 837
AfiI CCNNNNNNNGG 1 cut(s) 755
AflII CTTAAG 1 cut(s) 128
AgsI TTSAA 4 cut(s) 217, 247, 410, 694
AjuI GAANNNNNNNTTGG 2 cut(s) 261, 293
AluBI AGCT 8 cut(s) 223, 566, 578, 632, 770, 806, 913, 958
AluI AGCT 8 cut(s) 223, 566, 578, 632, 770, 806, 913, 958
Alw26I GTCTC 2 cut(s) 635, 797
AlwI GGATC 5 cut(s) 32, 499, 510, 658, 719
AoxI GGCC 3 cut(s) 67, 74, 483
ApeKI GCWGC 8 cut(s) 6, 141, 230, 251, 532, 575, 782, 910
ApoI RAATTY 3 cut(s) 47, 154, 707
AseI ATTAAT 1 cut(s) 716
Asp700I GAANNNNTTC 1 cut(s) 943
AspLEI GCGC 2 cut(s) 6, 162
AspS9I GGNCC 1 cut(s) 521
AsuHPI GGTGA 3 cut(s) 497, 610, 763
AvaII GGWCC 1 cut(s) 521
BaeGI GKGCMC 1 cut(s) 682
BalI TGGCCA 1 cut(s) 485
BbvI GCAGC 7 cut(s) 153, 217, 238, 544, 587, 794, 922
BcgI CGANNNNNNTGC 2 cut(s) 182, 216
BcoDI GTCTC 2 cut(s) 635, 797
BfaI CTAG 5 cut(s) 431, 512, 552, 668, 959
BfmI CTRYAG 1 cut(s) 965
BfrI CTTAAG 1 cut(s) 128
BglII AGATCT 1 cut(s) 961
BisI GCNGC 8 cut(s) 7, 142, 231, 252, 533, 576, 783, 911
BlsI GCNGC 8 cut(s) 8, 143, 232, 253, 534, 577, 784, 912
Bme18I GGWCC 1 cut(s) 521
BmgT120I GGNCC 1 cut(s) 521
BmsI GCATC 3 cut(s) 137, 318, 772
BoxI GACNNNNGTC 2 cut(s) 519, 920
Bpu10I CCTNAGC 1 cut(s) 734
BpuEI CTTGAG 3 cut(s) 158, 629, 866
BsaWI WCCGGW 3 cut(s) 348, 620, 748
Bsc4I CCNNNNNNNGG 1 cut(s) 755
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 1 cut(s) 486
Bse3DI GCAATG 1 cut(s) 792
BseGI GGATG 2 cut(s) 40, 561
BseLI CCNNNNNNNGG 1 cut(s) 755
BseMI GCAATG 1 cut(s) 792
BseMII CTCAG 1 cut(s) 228
BseNI ACTGG 1 cut(s) 486
BseSI GKGCMC 1 cut(s) 682
BseXI GCAGC 7 cut(s) 153, 217, 238, 544, 587, 794, 922
BsgI GTGCAG 1 cut(s) 929
BshFI GGCC 3 cut(s) 69, 76, 485
BsiSI CCGG 4 cut(s) 26, 349, 621, 749
BslI CCNNNNNNNGG 1 cut(s) 755
BsmAI GTCTC 2 cut(s) 635, 797
BsmI GAATGC 1 cut(s) 363
BsnI GGCC 3 cut(s) 69, 76, 485
Bsp1286I GDGCHC 1 cut(s) 682
Bsp1407I TGTACA 1 cut(s) 288
Bsp143I GATC 6 cut(s) 37, 491, 502, 663, 724, 961
BspACI CCGC 2 cut(s) 332, 446
BspANI GGCC 3 cut(s) 69, 76, 485
BspCNI CTCAG 1 cut(s) 229
BspPI GGATC 5 cut(s) 32, 499, 510, 658, 719
BspQI GCTCTTC 2 cut(s) 622, 651
BspTI CTTAAG 1 cut(s) 128
BsrDI GCAATG 1 cut(s) 792
BsrFI RCCGGY 1 cut(s) 25
BsrGI TGTACA 1 cut(s) 288
BsrI ACTGG 1 cut(s) 486
BssAI RCCGGY 1 cut(s) 25
BssMI GATC 6 cut(s) 37, 491, 502, 663, 724, 961
Bst4CI ACNGT 2 cut(s) 443, 690
Bst6I CTCTTC 2 cut(s) 622, 651
BstAFI CTTAAG 1 cut(s) 128
BstAPI GCANNNNNTGC 1 cut(s) 147
BstAUI TGTACA 1 cut(s) 288
BstC8I GCNNGC 1 cut(s) 23
BstDEI CTNAG 2 cut(s) 237, 734
BstF5I GGATG 2 cut(s) 40, 561
BstHHI GCGC 2 cut(s) 6, 162
BstKTI GATC 6 cut(s) 40, 494, 505, 666, 727, 964
BstMAI GTCTC 2 cut(s) 635, 797
BstMBI GATC 6 cut(s) 37, 491, 502, 663, 724, 961
BstMWI GCNNNNNNNGC 3 cut(s) 147, 572, 782
BstPAI GACNNNNGTC 2 cut(s) 519, 920
BstSFI CTRYAG 1 cut(s) 965
BstSLI GKGCMC 1 cut(s) 682
BstV1I GCAGC 7 cut(s) 153, 217, 238, 544, 587, 794, 922
BstX2I RGATCY 3 cut(s) 491, 663, 961
BstXI CCANNNNNNTGG 1 cut(s) 100
BstYI RGATCY 3 cut(s) 491, 663, 961
BsuRI GGCC 3 cut(s) 69, 76, 485
BtsCI GGATG 2 cut(s) 40, 561
BtsIMutI CAGTG 2 cut(s) 493, 686
Cac8I GCNNGC 1 cut(s) 23
CfoI GCGC 2 cut(s) 6, 162
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 1 cut(s) 521
Csp6I GTAC 5 cut(s) 289, 298, 439, 547, 836
CviAII CATG 2 cut(s) 227, 394
CviQI GTAC 5 cut(s) 289, 298, 439, 547, 836
DdeI CTNAG 2 cut(s) 237, 734
DpnI GATC 6 cut(s) 39, 493, 504, 665, 726, 963
DpnII GATC 6 cut(s) 37, 491, 502, 663, 724, 961
EaeI YGGCCR 1 cut(s) 483
Eam1104I CTCTTC 2 cut(s) 622, 651
EarI CTCTTC 2 cut(s) 622, 651
Eco47I GGWCC 1 cut(s) 521
Eco57I CTGAAG 2 cut(s) 405, 588
FaeI CATG 2 cut(s) 230, 397
FatI CATG 2 cut(s) 226, 393
Fnu4HI GCNGC 8 cut(s) 7, 142, 231, 252, 533, 576, 783, 911
FokI GGATG 2 cut(s) 47, 568
Fsp4HI GCNGC 8 cut(s) 7, 142, 231, 252, 533, 576, 783, 911
FspBI CTAG 5 cut(s) 431, 512, 552, 668, 959
FspI TGCGCA 1 cut(s) 161
GlaI GCGC 2 cut(s) 5, 161
GluI GCNGC 8 cut(s) 7, 142, 231, 252, 533, 576, 783, 911
HaeIII GGCC 3 cut(s) 69, 76, 485
HapII CCGG 4 cut(s) 26, 349, 621, 749
HhaI GCGC 2 cut(s) 6, 162
Hin1II CATG 2 cut(s) 230, 397
Hin6I GCGC 2 cut(s) 4, 160
HinP1I GCGC 2 cut(s) 4, 160
HinfI GANTC 4 cut(s) 104, 400, 526, 890
HpaII CCGG 4 cut(s) 26, 349, 621, 749
HphI GGTGA 3 cut(s) 497, 610, 763
Hpy166II GTNNAC 2 cut(s) 289, 549
Hpy188I TCNGA 5 cut(s) 45, 59, 168, 525, 829
Hpy188III TCNNGA 4 cut(s) 512, 539, 608, 845
Hpy8I GTNNAC 2 cut(s) 289, 549
HpyAV CCTTC 9 cut(s) 66, 263, 277, 443, 518, 563, 688, 748, 933
HpyCH4III ACNGT 2 cut(s) 443, 690
HpyCH4IV ACGT 1 cut(s) 212
HpyCH4V TGCA 9 cut(s) 21, 150, 188, 233, 361, 763, 776, 782, 910
HpyF10VI GCNNNNNNNGC 3 cut(s) 147, 572, 782
HpyF3I CTNAG 2 cut(s) 237, 734
HpySE526I ACGT 1 cut(s) 212
Hsp92II CATG 2 cut(s) 230, 397
HspAI GCGC 2 cut(s) 4, 160
Kzo9I GATC 6 cut(s) 37, 491, 502, 663, 724, 961
LguI GCTCTTC 2 cut(s) 622, 651
LmnI GCTCC 1 cut(s) 572
Lsp1109I GCAGC 7 cut(s) 153, 217, 238, 544, 587, 794, 922
LweI GCATC 3 cut(s) 137, 318, 772
MaeI CTAG 5 cut(s) 431, 512, 552, 668, 959
MaeII ACGT 1 cut(s) 212
MalI GATC 6 cut(s) 39, 493, 504, 665, 726, 963
MboI GATC 6 cut(s) 37, 491, 502, 663, 724, 961
MboII GAAGA 3 cut(s) 636, 639, 668
MfeI CAATTG 1 cut(s) 777
MflI RGATCY 3 cut(s) 491, 663, 961
MhlI GDGCHC 1 cut(s) 682
MlsI TGGCCA 1 cut(s) 485
MluCI AATT 6 cut(s) 47, 154, 313, 707, 717, 777
MluNI TGGCCA 1 cut(s) 485
MlyI GAGTC 2 cut(s) 98, 409
MnlI CCTC 8 cut(s) 25, 51, 232, 266, 493, 546, 646, 793
Mox20I TGGCCA 1 cut(s) 485
MroXI GAANNNNTTC 1 cut(s) 943
MscI TGGCCA 1 cut(s) 485
MseI TTAA 5 cut(s) 129, 336, 345, 614, 716
MslI CAYNNNNRTG 1 cut(s) 554
Msp20I TGGCCA 1 cut(s) 485
MspA1I CMGCKG 1 cut(s) 913
MspCI CTTAAG 1 cut(s) 128
MspI CCGG 4 cut(s) 26, 349, 621, 749
MunI CAATTG 1 cut(s) 777
Mva1269I GAATGC 1 cut(s) 363
MwoI GCNNNNNNNGC 3 cut(s) 147, 572, 782
NdeII GATC 6 cut(s) 37, 491, 502, 663, 724, 961
NlaIII CATG 2 cut(s) 230, 397
NmeAIII GCCGAG 1 cut(s) 181
NsbI TGCGCA 1 cut(s) 161
PciSI GCTCTTC 2 cut(s) 622, 651
PctI GAATGC 1 cut(s) 363
PdmI GAANNNNTTC 1 cut(s) 943
PfeI GAWTC 2 cut(s) 526, 890
PkrI GCNGC 8 cut(s) 8, 143, 232, 253, 534, 577, 784, 912
PleI GAGTC 2 cut(s) 98, 408
PpsI GAGTC 2 cut(s) 98, 408
PshAI GACNNNNGTC 2 cut(s) 519, 920
PshBI ATTAAT 1 cut(s) 716
PspPI GGNCC 1 cut(s) 521
PsuI RGATCY 3 cut(s) 491, 663, 961
PvuII CAGCTG 1 cut(s) 913
RsaI GTAC 5 cut(s) 290, 299, 440, 548, 837
RsaNI GTAC 5 cut(s) 289, 298, 439, 547, 836
RseI CAYNNNNRTG 1 cut(s) 554
SapI GCTCTTC 2 cut(s) 622, 651
SaqAI TTAA 5 cut(s) 129, 336, 345, 614, 716
SatI GCNGC 8 cut(s) 7, 142, 231, 252, 533, 576, 783, 911
Sau3AI GATC 6 cut(s) 37, 491, 502, 663, 724, 961
Sau96I GGNCC 1 cut(s) 521
SchI GAGTC 2 cut(s) 98, 409
SduI GDGCHC 1 cut(s) 682
SfaNI GCATC 3 cut(s) 137, 318, 772
SfcI CTRYAG 1 cut(s) 965
SinI GGWCC 1 cut(s) 521
SmiMI CAYNNNNRTG 1 cut(s) 554
SmlI CTYRAG 4 cut(s) 128, 173, 608, 845
SmoI CTYRAG 4 cut(s) 128, 173, 608, 845
Sse9I AATT 6 cut(s) 47, 154, 313, 707, 717, 777
SsiI CCGC 2 cut(s) 332, 446
SspMI CTAG 5 cut(s) 431, 512, 552, 668, 959
TaaI ACNGT 2 cut(s) 443, 690
TaiI ACGT 1 cut(s) 215
TasI AATT 6 cut(s) 47, 154, 313, 707, 717, 777
TatI WGTACW 2 cut(s) 288, 835
TfiI GAWTC 2 cut(s) 526, 890
Tru1I TTAA 5 cut(s) 129, 336, 345, 614, 716
Tru9I TTAA 5 cut(s) 129, 336, 345, 614, 716
TscAI CASTG 2 cut(s) 493, 693
TseI GCWGC 8 cut(s) 6, 141, 230, 251, 532, 575, 782, 910
TspDTI ATGAA 5 cut(s) 215, 489, 702, 885, 903
TspGWI ACGGA 1 cut(s) 717
TspRI CASTG 2 cut(s) 493, 693
Vha464I CTTAAG 1 cut(s) 128
VpaK11BI GGWCC 1 cut(s) 521
VspI ATTAAT 1 cut(s) 716
XapI RAATTY 3 cut(s) 47, 154, 707
XbaI TCTAGA 1 cut(s) 511
XcmI CCANNNNNNNNNTGG 1 cut(s) 493
XmnI GAANNNNTTC 1 cut(s) 943
XspI CTAG 5 cut(s) 431, 512, 552, 668, 959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.