MD10G1318000.v1.1
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0015787
Physical Location & Seq
Reverse (-)
40096445 .. 40098268
1824 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1318000.v1.1.491

Sequence Viewer

Length: 1233 bp
ATGACGGCTCTGCCAGCGGCAGAGGCGCCGGGGAGGGAGGAGAGCAGGATGACTTGCTTCTCATGTTGCAGGGTGGATGTCGACGAGCCTGAAGAGTCTGCGAGGAGGGCCAGAATAAGAAGGCCATACTGTAGTGGTGGGATGAGATTGAACTCGCTTTTAGCAACTTTTTCGGTTAAGTCAGGCAGCAGCCAATGCAGGAATTTACAGAAGGAGATACTAAAGATAGGACACGCGAAGAAATCTGCGCAGCTTTTTACATACGAGGAACTGGCTGCTGCAACTGATAACTTCAATCCTGATTCTCTGCTGGGAGAAGGTGGATTTGGGAAGGTGTACAAAGGTTACTTGGACAGCATTAAACAGATTGTGGCGGTAAAGAAACTCGACAGGAAAGGGTTGCAAGGGAGCAGAGAGTTCTGCTCTGAAGTTATCATGTTGAGTTTGGTTCAACACGCGAACCTCGTCAATCTTATTGGCTATTGTGCAGAAGGAGATCAGAGAATTTTAGTGTATGAATTTATGGTCAATGGATCTTTGGAGAATCACCTTCTAGATATATATCCAAATAGGGAGCCTCTTGATTGGTACACAAGGATGAAAATAGCTGAAGGAGCAGCAAAAGGGCTCGAGTACTTGCACGAAACTGCCAATCCGCCAGTGATTTACCGTGATTTGAAAGCTTCAAACATTTTACTAGACGAAAAATTATGTCCAAAGCTCTCTGATTTTGGACTTGCTAAGTTAGGTCCAACCAGAGACAAGGACCATGTAACTACTAGGGTGATGGGGACTTTCGGCTACTGTGCACCGGAGTATGCTTCAACAGGACAGCTGACAATAATGTCCGATGTCTACAGCTTCGGAGTTGTGTTTCCCGAGTTAATCACAGGAAGGAGAGCTATTGATGATCAAAGACCGGTTGGGGAGCAGATCCTAGTCATTTGGGCGAAGCCAAAGCTAAAGGACAGAAAGAGATACGCATCGATGGTTGACCCGTTACTTCGAGGGAGATATCCGGCGAAGGGTCTGAACCAAGCCGTTGCAATTGCAGCAATGTGTCTGAACGAGGAAGCTGATTACCGGCCTGCGATGGGTGATGTTGTGACCGCTCTCAGGCACCTATCGTTGCCAGTATACGACAGAAAAGGTGCCAAGGGTGCATTGGGAAACAGAGGCGCGCACGTTGAGTCAGTAAGCAGAGGAAGTTTCGGAGGAGACAAAGAGCTATAG

Protein Analysis

411

Amino Acids

45.37

Weight (kDa)

8.66

Isoelectric Point (pI)

31.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 101 - 307 1.1e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 102 - 371 1.8e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015787)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61860
fragaria_vesca FvH4_3g02440
malus_domestica MD05G1343000.v1.1 MD10G1318000.v1.1
prunus_persica Prupe.4G023800_v2.0.a1
pyrus_communis pycom05g31250
rosa_chinensis RchiOBHm_Chr5g0003691
rosa_laevigata RLG00000031166
rosa_multiflora Rmu_sc0000500.1_g000001
rosa_rugosa Rorug04G0405900
rosa_samantha Rh5AG032700 Rh5BG031300 Rh5CG035400 Rh5DG031900
rosa_wichuraiana Rw5G003030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 844
Acc16I TGCGCA 1 cut(s) 249
AccB1I GGYRCC 3 cut(s) 25, 1119, 1151
AccBSI CCGCTC 1 cut(s) 1112
AccI GTMKAC 3 cut(s) 81, 855, 1137
AccII CGCG 3 cut(s) 236, 458, 1181
AciI CCGC 4 cut(s) 17, 374, 656, 1110
AclWI GGATC 2 cut(s) 541, 928
AcsI RAATTY 3 cut(s) 202, 504, 518
AcuI CTGAAG 3 cut(s) 111, 447, 630
AcyI GRCGYC 1 cut(s) 26
AfaI GTAC 3 cut(s) 338, 590, 635
AfiI CCNNNNNNNGG 3 cut(s) 1025, 1094, 1116
AgeI ACCGGT 1 cut(s) 919
AgsI TTSAA 6 cut(s) 151, 295, 452, 679, 687, 825
AjuI GAANNNNNNNTTGG 2 cut(s) 309, 341
Alw21I GWGCWC 1 cut(s) 811
Alw26I GTCTC 2 cut(s) 753, 1212
Alw44I GTGCAC 1 cut(s) 807
AlwI GGATC 2 cut(s) 541, 928
Ama87I CYCGRG 2 cut(s) 629, 878
AoxI GGCC 3 cut(s) 108, 122, 1085
ApaLI GTGCAC 1 cut(s) 807
ApeKI GCWGC 7 cut(s) 186, 189, 250, 275, 278, 617, 1052
ApoI RAATTY 3 cut(s) 202, 504, 518
AsiGI ACCGGT 1 cut(s) 919
AspLEI GCGC 4 cut(s) 28, 250, 1181, 1183
AspS9I GGNCC 3 cut(s) 108, 749, 766
AsuC2I CCSGG 1 cut(s) 30
AsuHPI GGTGA 3 cut(s) 539, 796, 1109
AvaI CYCGRG 2 cut(s) 629, 878
AvaII GGWCC 2 cut(s) 749, 766
BaeGI GKGCMC 1 cut(s) 811
BanI GGYRCC 3 cut(s) 25, 1119, 1151
BanII GRGCYC 1 cut(s) 630
Bbv12I GWGCWC 1 cut(s) 811
BbvI GCAGC 7 cut(s) 198, 201, 262, 262, 265, 629, 1064
BccI CCATC 3 cut(s) 781, 982, 1087
BceAI ACGGC 2 cut(s) 21, 1025
BcgI CGANNNNNNTGC 2 cut(s) 153, 187
BclI TGATCA 1 cut(s) 910
BcnI CCSGG 1 cut(s) 30
BcoDI GTCTC 2 cut(s) 753, 1212
BfaI CTAG 4 cut(s) 554, 698, 780, 938
BfmI CTRYAG 3 cut(s) 130, 856, 1229
BfoI RGCGCY 1 cut(s) 29
BisI GCNGC 8 cut(s) 18, 187, 190, 251, 276, 279, 618, 1053
BlsI GCNGC 8 cut(s) 19, 188, 191, 252, 277, 280, 619, 1054
BmcAI AGTACT 1 cut(s) 635
Bme1390I CCNGG 1 cut(s) 30
Bme18I GGWCC 2 cut(s) 749, 766
BmeT110I CYCGRG 2 cut(s) 629, 878
BmgT120I GGNCC 3 cut(s) 108, 749, 766
BmiI GGNNCC 4 cut(s) 27, 576, 1121, 1153
BmrFI CCNGG 1 cut(s) 30
BmsI GCATC 1 cut(s) 992
BplI GAGNNNNNCTC 2 cut(s) 407, 439
BpuMI CCSGG 1 cut(s) 30
Bsa29I ATCGAT 1 cut(s) 986
BsaBI GATNNNNATC 2 cut(s) 561, 982
BsaHI GRCGYC 1 cut(s) 26
BsaJI CCNNGG 2 cut(s) 29, 1155
BsaWI WCCGGW 2 cut(s) 811, 919
Bsc4I CCNNNNNNNGG 3 cut(s) 1025, 1094, 1116
Bse118I RCCGGY 2 cut(s) 919, 1083
Bse1I ACTGG 3 cut(s) 276, 659, 1133
Bse3DI GCAATG 1 cut(s) 1062
Bse8I GATNNNNATC 2 cut(s) 561, 982
BseCI ATCGAT 1 cut(s) 986
BseDI CCNNGG 2 cut(s) 29, 1155
BseGI GGATG 4 cut(s) 54, 82, 147, 603
BseJI GATNNNNATC 2 cut(s) 561, 982
BseLI CCNNNNNNNGG 3 cut(s) 1025, 1094, 1116
BseMI GCAATG 1 cut(s) 1062
BseMII CTCAG 1 cut(s) 1129
BseNI ACTGG 3 cut(s) 276, 659, 1133
BsePI GCGCGC 1 cut(s) 1179
BseRI GAGGAG 3 cut(s) 53, 118, 1230
BseSI GKGCMC 1 cut(s) 811
BseXI GCAGC 7 cut(s) 198, 201, 262, 262, 265, 629, 1064
BseYI CCCAGC 1 cut(s) 310
BsgI GTGCAG 1 cut(s) 507
Bsh1236I CGCG 3 cut(s) 236, 458, 1181
BshFI GGCC 3 cut(s) 110, 124, 1087
BshNI GGYRCC 3 cut(s) 25, 1119, 1151
BshTI ACCGGT 1 cut(s) 919
BshVI ATCGAT 1 cut(s) 986
BsiHKAI GWGCWC 1 cut(s) 811
BsiHKCI CYCGRG 2 cut(s) 629, 878
BsiSI CCGG 5 cut(s) 29, 812, 920, 1019, 1084
BslFI GGGAC 1 cut(s) 805
BslI CCNNNNNNNGG 3 cut(s) 1025, 1094, 1116
BsmAI GTCTC 2 cut(s) 753, 1212
BsmFI GGGAC 1 cut(s) 805
BsnI GGCC 3 cut(s) 110, 124, 1087
BsoBI CYCGRG 2 cut(s) 629, 878
Bsp1286I GDGCHC 2 cut(s) 630, 811
Bsp1407I TGTACA 1 cut(s) 336
Bsp143I GATC 4 cut(s) 496, 533, 910, 933
BspACI CCGC 4 cut(s) 17, 374, 656, 1110
BspANI GGCC 3 cut(s) 110, 124, 1087
BspCNI CTCAG 1 cut(s) 1128
BspDI ATCGAT 1 cut(s) 986
BspFNI CGCG 3 cut(s) 236, 458, 1181
BspLI GGNNCC 4 cut(s) 27, 576, 1121, 1153
BspPI GGATC 2 cut(s) 541, 928
BspT107I GGYRCC 3 cut(s) 25, 1119, 1151
BsrBI CCGCTC 1 cut(s) 1112
BsrDI GCAATG 1 cut(s) 1062
BsrFI RCCGGY 2 cut(s) 919, 1083
BsrGI TGTACA 1 cut(s) 336
BsrI ACTGG 3 cut(s) 276, 659, 1133
BssAI RCCGGY 2 cut(s) 919, 1083
BssECI CCNNGG 2 cut(s) 29, 1155
BssHII GCGCGC 1 cut(s) 1179
BssMI GATC 4 cut(s) 496, 533, 910, 933
BssNAI GTATAC 1 cut(s) 1138
BssNI GRCGYC 1 cut(s) 26
BssT1I CCWWGG 1 cut(s) 1155
Bst1107I GTATAC 1 cut(s) 1138
Bst4CI ACNGT 3 cut(s) 131, 671, 806
Bst6I CTCTTC 1 cut(s) 87
BstACI GRCGYC 1 cut(s) 26
BstAPI GCANNNNNTGC 1 cut(s) 195
BstAUI TGTACA 1 cut(s) 336
BstC8I GCNNGC 3 cut(s) 15, 1089, 1181
BstDEI CTNAG 2 cut(s) 741, 1115
BstF5I GGATG 4 cut(s) 54, 82, 147, 603
BstFNI CGCG 3 cut(s) 236, 458, 1181
BstH2I RGCGCY 1 cut(s) 29
BstHHI GCGC 4 cut(s) 28, 250, 1181, 1183
BstKTI GATC 4 cut(s) 499, 536, 913, 936
BstMAI GTCTC 2 cut(s) 753, 1212
BstMBI GATC 4 cut(s) 496, 533, 910, 933
BstMWI GCNNNNNNNGC 7 cut(s) 14, 23, 107, 195, 614, 1052, 1160
BstSCI CCNGG 1 cut(s) 28
BstSFI CTRYAG 3 cut(s) 130, 856, 1229
BstSLI GKGCMC 1 cut(s) 811
BstUI CGCG 3 cut(s) 236, 458, 1181
BstV1I GCAGC 7 cut(s) 198, 201, 262, 262, 265, 629, 1064
BstX2I RGATCY 2 cut(s) 533, 933
BstYI RGATCY 2 cut(s) 533, 933
BstZ17I GTATAC 1 cut(s) 1138
Bsu15I ATCGAT 1 cut(s) 986
BsuRI GGCC 3 cut(s) 110, 124, 1087
BsuTUI ATCGAT 1 cut(s) 986
BtgZI GCGATG 1 cut(s) 1106
BtsCI GGATG 4 cut(s) 54, 82, 147, 603
BtsIMutI CAGTG 1 cut(s) 666
Cac8I GCNNGC 3 cut(s) 15, 1089, 1181
CfoI GCGC 4 cut(s) 28, 250, 1181, 1183
Cfr10I RCCGGY 2 cut(s) 919, 1083
Cfr13I GGNCC 3 cut(s) 108, 749, 766
ClaI ATCGAT 1 cut(s) 986
Csp6I GTAC 3 cut(s) 337, 589, 634
CspAI ACCGGT 1 cut(s) 919
CviAII CATG 3 cut(s) 63, 436, 770
CviQI GTAC 3 cut(s) 337, 589, 634
DdeI CTNAG 2 cut(s) 741, 1115
DinI GGCGCC 1 cut(s) 27
DpnI GATC 4 cut(s) 498, 535, 912, 935
DpnII GATC 4 cut(s) 496, 533, 910, 933
DrdI GACNNNNNNGTC 1 cut(s) 844
DseDI GACNNNNNNGTC 1 cut(s) 844
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
EciI GGCGGA 1 cut(s) 645
Eco130I CCWWGG 1 cut(s) 1155
Eco24I GRGCYC 1 cut(s) 630
Eco32I GATATC 1 cut(s) 1016
Eco47I GGWCC 2 cut(s) 749, 766
Eco57I CTGAAG 3 cut(s) 111, 447, 630
Eco88I CYCGRG 2 cut(s) 629, 878
EcoRV GATATC 1 cut(s) 1016
EcoT14I CCWWGG 1 cut(s) 1155
EcoT38I GRGCYC 1 cut(s) 630
EgeI GGCGCC 1 cut(s) 27
EheI GGCGCC 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 1155
FaeI CATG 3 cut(s) 66, 439, 773
FaqI GGGAC 1 cut(s) 805
FatI CATG 3 cut(s) 62, 435, 769
FbaI TGATCA 1 cut(s) 910
FblI GTMKAC 3 cut(s) 81, 855, 1137
Fnu4HI GCNGC 8 cut(s) 18, 187, 190, 251, 276, 279, 618, 1053
FokI GGATG 4 cut(s) 61, 89, 154, 610
FriOI GRGCYC 1 cut(s) 630
Fsp4HI GCNGC 8 cut(s) 18, 187, 190, 251, 276, 279, 618, 1053
FspBI CTAG 4 cut(s) 554, 698, 780, 938
FspI TGCGCA 1 cut(s) 249
GlaI GCGC 4 cut(s) 27, 249, 1180, 1182
GluI GCNGC 8 cut(s) 18, 187, 190, 251, 276, 279, 618, 1053
GsaI CCCAGC 1 cut(s) 314
HaeII RGCGCY 1 cut(s) 29
HaeIII GGCC 3 cut(s) 110, 124, 1087
HapII CCGG 5 cut(s) 29, 812, 920, 1019, 1084
HhaI GCGC 4 cut(s) 28, 250, 1181, 1183
Hin1I GRCGYC 1 cut(s) 26
Hin1II CATG 3 cut(s) 66, 439, 773
Hin6I GCGC 4 cut(s) 26, 248, 1179, 1181
HinP1I GCGC 4 cut(s) 26, 248, 1179, 1181
HincII GTYRAC 2 cut(s) 82, 994
HindII GTYRAC 2 cut(s) 82, 994
HindIII AAGCTT 1 cut(s) 681
HinfI GANTC 4 cut(s) 95, 302, 544, 1190
HpaII CCGG 5 cut(s) 29, 812, 920, 1019, 1084
HphI GGTGA 3 cut(s) 539, 796, 1109
Hpy166II GTNNAC 7 cut(s) 82, 337, 591, 809, 856, 994, 1138
Hpy188I TCNGA 8 cut(s) 427, 501, 727, 850, 866, 1032, 1065, 1214
Hpy188III TCNNGA 4 cut(s) 299, 554, 581, 878
Hpy8I GTNNAC 7 cut(s) 82, 337, 591, 809, 856, 994, 1138
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 9 cut(s) 114, 205, 311, 325, 485, 560, 605, 888, 1018
HpyCH4III ACNGT 3 cut(s) 131, 671, 806
HpyCH4IV ACGT 1 cut(s) 1185
HpyF10VI GCNNNNNNNGC 7 cut(s) 14, 23, 107, 195, 614, 1052, 1160
HpyF3I CTNAG 2 cut(s) 741, 1115
HpySE526I ACGT 1 cut(s) 1185
Hsp92I GRCGYC 1 cut(s) 26
Hsp92II CATG 3 cut(s) 66, 439, 773
HspAI GCGC 4 cut(s) 26, 248, 1179, 1181
KasI GGCGCC 1 cut(s) 25
Ksp22I TGATCA 1 cut(s) 910
Kzo9I GATC 4 cut(s) 496, 533, 910, 933
LmnI GCTCC 4 cut(s) 408, 574, 614, 928
Lsp1109I GCAGC 7 cut(s) 198, 201, 262, 262, 265, 629, 1064
LweI GCATC 1 cut(s) 992
MaeI CTAG 4 cut(s) 554, 698, 780, 938
MaeII ACGT 1 cut(s) 1185
MaeIII GTNAC 4 cut(s) 344, 772, 999, 1105
MalI GATC 4 cut(s) 498, 535, 912, 935
MbiI CCGCTC 1 cut(s) 1112
MboI GATC 4 cut(s) 496, 533, 910, 933
MboII GAAGA 2 cut(s) 104, 250
MfeI CAATTG 1 cut(s) 1047
MflI RGATCY 2 cut(s) 533, 933
MhlI GDGCHC 2 cut(s) 630, 811
MluCI AATT 5 cut(s) 202, 504, 518, 707, 1047
Mly113I GGCGCC 1 cut(s) 26
MlyI GAGTC 2 cut(s) 104, 1199
MmeI TCCRAC 1 cut(s) 776
MseI TTAA 3 cut(s) 177, 360, 884
MslI CAYNNNNRTG 1 cut(s) 596
MspA1I CMGCKG 2 cut(s) 17, 835
MspI CCGG 5 cut(s) 29, 812, 920, 1019, 1084
MspR9I CCNGG 1 cut(s) 30
MunI CAATTG 1 cut(s) 1047
MvnI CGCG 3 cut(s) 236, 458, 1181
MwoI GCNNNNNNNGC 7 cut(s) 14, 23, 107, 195, 614, 1052, 1160
NarI GGCGCC 1 cut(s) 26
NciI CCSGG 1 cut(s) 30
NdeII GATC 4 cut(s) 496, 533, 910, 933
NlaIII CATG 3 cut(s) 66, 439, 773
NlaIV GGNNCC 4 cut(s) 27, 576, 1121, 1153
NmuCI GTSAC 1 cut(s) 1105
NsbI TGCGCA 1 cut(s) 249
PaeR7I CTCGAG 1 cut(s) 629
PauI GCGCGC 1 cut(s) 1179
PcsI WCGNNNNNNNCGW 1 cut(s) 462
PfeI GAWTC 2 cut(s) 302, 544
PinAI ACCGGT 1 cut(s) 919
PkrI GCNGC 8 cut(s) 19, 188, 191, 252, 277, 280, 619, 1054
PleI GAGTC 2 cut(s) 103, 1198
PluTI GGCGCC 1 cut(s) 29
PpsI GAGTC 2 cut(s) 103, 1198
PspFI CCCAGC 1 cut(s) 310
PspN4I GGNNCC 4 cut(s) 27, 576, 1121, 1153
PspPI GGNCC 3 cut(s) 108, 749, 766
PspXI VCTCGAGB 1 cut(s) 629
PsuI RGATCY 2 cut(s) 533, 933
PteI GCGCGC 1 cut(s) 1179
PvuII CAGCTG 1 cut(s) 835
RsaI GTAC 3 cut(s) 338, 590, 635
RsaNI GTAC 3 cut(s) 337, 589, 634
RseI CAYNNNNRTG 1 cut(s) 596
SalI GTCGAC 1 cut(s) 80
SaqAI TTAA 3 cut(s) 177, 360, 884
SatI GCNGC 8 cut(s) 18, 187, 190, 251, 276, 279, 618, 1053
Sau3AI GATC 4 cut(s) 496, 533, 910, 933
Sau96I GGNCC 3 cut(s) 108, 749, 766
ScaI AGTACT 1 cut(s) 635
SchI GAGTC 2 cut(s) 104, 1199
ScrFI CCNGG 1 cut(s) 30
SduI GDGCHC 2 cut(s) 630, 811
SfaNI GCATC 1 cut(s) 992
SfcI CTRYAG 3 cut(s) 130, 856, 1229
SfoI GGCGCC 1 cut(s) 27
Sfr274I CTCGAG 1 cut(s) 629
SinI GGWCC 2 cut(s) 749, 766
SlaI CTCGAG 1 cut(s) 629
SmiMI CAYNNNNRTG 1 cut(s) 596
SmlI CTYRAG 1 cut(s) 629
SmoI CTYRAG 1 cut(s) 629
Sse9I AATT 5 cut(s) 202, 504, 518, 707, 1047
SsiI CCGC 4 cut(s) 17, 374, 656, 1110
SspDI GGCGCC 1 cut(s) 25
SspMI CTAG 4 cut(s) 554, 698, 780, 938
StyD4I CCNGG 1 cut(s) 28
StyI CCWWGG 1 cut(s) 1155
TaaI ACNGT 3 cut(s) 131, 671, 806
TaiI ACGT 1 cut(s) 1188
TaqI TCGA 5 cut(s) 81, 387, 630, 986, 1006
TasI AATT 5 cut(s) 202, 504, 518, 707, 1047
TatI WGTACW 2 cut(s) 336, 633
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 2 cut(s) 302, 544
Tru1I TTAA 3 cut(s) 177, 360, 884
Tru9I TTAA 3 cut(s) 177, 360, 884
TscAI CASTG 1 cut(s) 666
TseFI GTSAC 1 cut(s) 1105
TseI GCWGC 7 cut(s) 186, 189, 250, 275, 278, 617, 1052
Tsp45I GTSAC 1 cut(s) 1105
TspDTI ATGAA 2 cut(s) 531, 614
TspRI CASTG 1 cut(s) 666
VneI GTGCAC 1 cut(s) 807
VpaK11BI GGWCC 2 cut(s) 749, 766
XapI RAATTY 3 cut(s) 202, 504, 518
XbaI TCTAGA 1 cut(s) 553
XcmI CCANNNNNNNNNTGG 1 cut(s) 1162
XhoI CTCGAG 1 cut(s) 629
XmiI GTMKAC 3 cut(s) 81, 855, 1137
XspI CTAG 4 cut(s) 554, 698, 780, 938
ZrmI AGTACT 1 cut(s) 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.