pycom05g31250
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0015787
Physical Location & Seq
Reverse (-)
31064897 .. 31066727
1831 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g31250.4

Sequence Viewer

Length: 1293 bp
ATGACTTGCTTCTCGTGTTGCAAGGTGGACGACGAGCCTGAAGAATCTGTGAGGAGGGCCAGAATAAGAAGGCAATACTCAAGTGGTTTGAAGAGATTGACCTCGCTTTTAGCAACTTTTTCGGTTAAGTCAGGTAGCAACGAATGCAGGAATTTACAGAAGGAGATACTCAAGACGGGACACGCGAAAAAAGTCGCTCAGCTTTTTACGTACGAGGAACTAGCTGCTGCAACTGATAACTTCAATCCTGATTCTCTGCTGGGAGAAGGTGGATTTGGGAAGGTGTACAGAGGTTACTTGGACAGCATTAAACAAATTGTGGCAGTGAAGAAACTTGACAGGAAAGGATTGCAAGGGAGCAGAGAGTTTTGTTCTGAAGTTATCATGTTGAGTTTGGTTCAACACGCGAACCTCGTCAATCTTATTGGCTATTGTGCAGAAGGAGACCAGAGAATGTTAGTGTATGAATTCATGGTCAATGGATCTTTGGAGAATCACCTTCTAAGTATGCACAAAAATTATTTGGATCCCGAAAATTTTTATGTATATTCGAATATGGAGCCTCTTGATTGGTATACGAGGATGAAAATAGCTGAAGGAGCAGCTAAAGGGCTCGAGTACCTTCATGAGACTGCCAATCCGCCAGTGATTTATCGCGACTTCAAAGCTTCAAACATTATACTAGACGAAAAATTCTGTCCTAAGCTTTCTGATTTTGGACTTGCTAAGTTAGGTCCAACCGGAGACAAGGACCATGTGACTACTAGAGTGATGGGGACTTATGGCTATTGTGCACCAGAGTATGCTTCAACAGGACAGCTGACAACTAGGTCCGATGTCTACAGCTTTGGCGTTGTGCTTCTTGAGTTGATCACAGGACGGAGAGCTATTGATGAGCGAAGACCGGTTGGACAGCAGATCCTAGTCAGTTGGGTATGTGAACATTCATCAAGTAGCAAAAGCCTTTACGAAGTTACTTGTTACTGTCTCTTTATCGAATCGTATAAGCAGTCTGCAAAGCCAATGCTTAAGGACAGAAAGAGATACGCGTCGATGGCTGACCCGTTGCTTCGAGGCAGATTTCCGGCAAAGGGTCTGAACCAAGCTGTTGCAATTGCTGCAATGTGTCTGAATGAGGAAGCTGATTGCCGGCCTTCGATGGGTGATGTTGTGACCGCTCTCGGGCACCTATCGTTGCCAGACTACGATGGAGAAGGTGCCAAGGGTGCATCGAGAAACAGAGGCAGGCACGTTGAGTCAATAAGAAGCGCAAGAGGAGACAAAGAGCTATAG

Protein Analysis

431

Amino Acids

48.09

Weight (kDa)

8.3

Isoelectric Point (pI)

36.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015787)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61860
fragaria_vesca FvH4_3g02440
malus_domestica MD05G1343000.v1.1 MD10G1318000.v1.1
prunus_persica Prupe.4G023800_v2.0.a1
pyrus_communis pycom05g31250
rosa_chinensis RchiOBHm_Chr5g0003691
rosa_laevigata RLG00000031166
rosa_multiflora Rmu_sc0000500.1_g000001
rosa_rugosa Rorug04G0405900
rosa_samantha Rh5AG032700 Rh5BG031300 Rh5CG035400 Rh5DG031900
rosa_wichuraiana Rw5G003030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 829
AccB1I GGYRCC 2 cut(s) 1185, 1217
AccBSI CCGCTC 1 cut(s) 1178
AccI GTMKAC 2 cut(s) 575, 840
AccII CGCG 4 cut(s) 185, 407, 657, 1049
AciI CCGC 2 cut(s) 641, 1176
AclWI GGATC 4 cut(s) 490, 521, 534, 913
AcsI RAATTY 4 cut(s) 151, 467, 535, 692
AcuI CTGAAG 3 cut(s) 60, 396, 615
AfaI GTAC 3 cut(s) 212, 287, 620
AfiI CCNNNNNNNGG 3 cut(s) 1091, 1160, 1182
AflII CTTAAG 1 cut(s) 1028
AflIII ACRYGT 1 cut(s) 1047
AgeI ACCGGT 1 cut(s) 904
AgsI TTSAA 6 cut(s) 91, 244, 401, 664, 672, 810
AjuI GAANNNNNNNTTGG 2 cut(s) 258, 290
Alw21I GWGCWC 1 cut(s) 796
Alw26I GTCTC 5 cut(s) 438, 623, 738, 992, 1272
Alw44I GTGCAC 1 cut(s) 792
AlwI GGATC 4 cut(s) 490, 521, 534, 913
Ama87I CYCGRG 2 cut(s) 614, 1181
AoxI GGCC 2 cut(s) 57, 1151
ApaLI GTGCAC 1 cut(s) 792
ApeKI GCWGC 4 cut(s) 224, 227, 602, 1118
ApoI RAATTY 4 cut(s) 151, 467, 535, 692
AsiGI ACCGGT 1 cut(s) 904
AspLEI GCGC 1 cut(s) 1271
AspS9I GGNCC 4 cut(s) 57, 734, 751, 831
AsuHPI GGTGA 2 cut(s) 488, 1175
AsuII TTCGAA 1 cut(s) 551
AvaI CYCGRG 2 cut(s) 614, 1181
AvaII GGWCC 3 cut(s) 734, 751, 831
BaeGI GKGCMC 2 cut(s) 796, 1188
BamHI GGATCC 1 cut(s) 526
BanI GGYRCC 2 cut(s) 1185, 1217
BanII GRGCYC 1 cut(s) 615
BauI CACGAG 1 cut(s) 13
BbsI GAAGAC 1 cut(s) 907
Bbv12I GWGCWC 1 cut(s) 796
BbvI GCAGC 4 cut(s) 211, 214, 614, 1105
BccI CCATC 4 cut(s) 766, 1048, 1153, 1202
BcgI CGANNNNNNTGC 2 cut(s) 102, 136
BclI TGATCA 1 cut(s) 870
BcoDI GTCTC 5 cut(s) 438, 623, 738, 992, 1272
BfaI CTAG 5 cut(s) 221, 683, 765, 828, 923
BfmI CTRYAG 2 cut(s) 841, 1289
BfrI CTTAAG 1 cut(s) 1028
BisI GCNGC 4 cut(s) 225, 228, 603, 1119
BlpI GCTNAGC 1 cut(s) 198
BlsI GCNGC 4 cut(s) 226, 229, 604, 1120
Bme18I GGWCC 3 cut(s) 734, 751, 831
BmeT110I CYCGRG 2 cut(s) 614, 1181
BmgT120I GGNCC 4 cut(s) 57, 734, 751, 831
BmiI GGNNCC 4 cut(s) 528, 561, 1187, 1219
BmsI GCATC 1 cut(s) 1238
BpiI GAAGAC 1 cut(s) 907
Bpu10I CCTNAGC 1 cut(s) 702
Bpu1102I GCTNAGC 1 cut(s) 198
Bpu14I TTCGAA 1 cut(s) 551
BpuEI CTTGAG 3 cut(s) 64, 155, 884
BsaAI YACGTR 1 cut(s) 210
BsaI GGTCTC 1 cut(s) 438
BsaJI CCNNGG 1 cut(s) 1221
BsaWI WCCGGW 2 cut(s) 740, 904
Bsc4I CCNNNNNNNGG 3 cut(s) 1091, 1160, 1182
Bse118I RCCGGY 2 cut(s) 904, 1149
Bse1I ACTGG 1 cut(s) 644
Bse3DI GCAATG 1 cut(s) 1128
BseDI CCNNGG 1 cut(s) 1221
BseGI GGATG 1 cut(s) 588
BseLI CCNNNNNNNGG 3 cut(s) 1091, 1160, 1182
BseMI GCAATG 1 cut(s) 1128
BseMII CTCAG 1 cut(s) 212
BseNI ACTGG 1 cut(s) 644
BseRI GAGGAG 2 cut(s) 67, 1290
BseSI GKGCMC 2 cut(s) 796, 1188
BseXI GCAGC 4 cut(s) 211, 214, 614, 1105
BseYI CCCAGC 1 cut(s) 259
BsgI GTGCAG 1 cut(s) 456
Bsh1236I CGCG 4 cut(s) 185, 407, 657, 1049
BshFI GGCC 2 cut(s) 59, 1153
BshNI GGYRCC 2 cut(s) 1185, 1217
BshTI ACCGGT 1 cut(s) 904
BsiHKAI GWGCWC 1 cut(s) 796
BsiHKCI CYCGRG 2 cut(s) 614, 1181
BsiSI CCGG 4 cut(s) 741, 905, 1085, 1150
BsiWI CGTACG 1 cut(s) 210
BslFI GGGAC 2 cut(s) 192, 790
BslI CCNNNNNNNGG 3 cut(s) 1091, 1160, 1182
BsmAI GTCTC 5 cut(s) 438, 623, 738, 992, 1272
BsmFI GGGAC 2 cut(s) 192, 790
BsmI GAATGC 1 cut(s) 149
BsnI GGCC 2 cut(s) 59, 1153
Bso31I GGTCTC 1 cut(s) 438
BsoBI CYCGRG 2 cut(s) 614, 1181
Bsp119I TTCGAA 1 cut(s) 551
Bsp1286I GDGCHC 3 cut(s) 615, 796, 1188
Bsp1407I TGTACA 1 cut(s) 285
Bsp143I GATC 4 cut(s) 482, 526, 870, 918
Bsp1720I GCTNAGC 1 cut(s) 198
Bsp68I TCGCGA 1 cut(s) 657
BspACI CCGC 2 cut(s) 641, 1176
BspANI GGCC 2 cut(s) 59, 1153
BspCNI CTCAG 1 cut(s) 211
BspFNI CGCG 4 cut(s) 185, 407, 657, 1049
BspHI TCATGA 1 cut(s) 625
BspLI GGNNCC 4 cut(s) 528, 561, 1187, 1219
BspPI GGATC 4 cut(s) 490, 521, 534, 913
BspT104I TTCGAA 1 cut(s) 551
BspT107I GGYRCC 2 cut(s) 1185, 1217
BspTI CTTAAG 1 cut(s) 1028
BspTNI GGTCTC 1 cut(s) 438
BsrBI CCGCTC 1 cut(s) 1178
BsrDI GCAATG 1 cut(s) 1128
BsrFI RCCGGY 2 cut(s) 904, 1149
BsrGI TGTACA 1 cut(s) 285
BsrI ACTGG 1 cut(s) 644
BssAI RCCGGY 2 cut(s) 904, 1149
BssECI CCNNGG 1 cut(s) 1221
BssMI GATC 4 cut(s) 482, 526, 870, 918
BssNAI GTATAC 1 cut(s) 576
BssSI CACGAG 1 cut(s) 13
BssT1I CCWWGG 1 cut(s) 1221
Bst1107I GTATAC 1 cut(s) 576
Bst2BI CACGAG 1 cut(s) 13
Bst4CI ACNGT 1 cut(s) 986
Bst6I CTCTTC 1 cut(s) 86
BstAFI CTTAAG 1 cut(s) 1028
BstAPI GCANNNNNTGC 2 cut(s) 144, 1118
BstAUI TGTACA 1 cut(s) 285
BstBAI YACGTR 1 cut(s) 210
BstBI TTCGAA 1 cut(s) 551
BstC8I GCNNGC 2 cut(s) 1151, 1247
BstDEI CTNAG 4 cut(s) 198, 503, 702, 726
BstF5I GGATG 1 cut(s) 588
BstFNI CGCG 4 cut(s) 185, 407, 657, 1049
BstHHI GCGC 1 cut(s) 1271
BstKTI GATC 4 cut(s) 485, 529, 873, 921
BstMAI GTCTC 5 cut(s) 438, 623, 738, 992, 1272
BstMBI GATC 4 cut(s) 482, 526, 870, 918
BstMWI GCNNNNNNNGC 5 cut(s) 144, 599, 1055, 1118, 1226
BstSFI CTRYAG 2 cut(s) 841, 1289
BstSLI GKGCMC 2 cut(s) 796, 1188
BstSNI TACGTA 1 cut(s) 210
BstUI CGCG 4 cut(s) 185, 407, 657, 1049
BstV1I GCAGC 4 cut(s) 211, 214, 614, 1105
BstV2I GAAGAC 1 cut(s) 907
BstX2I RGATCY 3 cut(s) 482, 526, 918
BstYI RGATCY 3 cut(s) 482, 526, 918
BstZ17I GTATAC 1 cut(s) 576
BsuRI GGCC 2 cut(s) 59, 1153
BtsCI GGATG 1 cut(s) 588
BtsI GCAGTG 1 cut(s) 330
BtsIMutI CAGTG 2 cut(s) 330, 651
BtuMI TCGCGA 1 cut(s) 657
Cac8I GCNNGC 2 cut(s) 1151, 1247
CciI TCATGA 1 cut(s) 625
CfoI GCGC 1 cut(s) 1271
Cfr10I RCCGGY 2 cut(s) 904, 1149
Cfr13I GGNCC 4 cut(s) 57, 734, 751, 831
CseI GACGC 1 cut(s) 1038
Csp6I GTAC 3 cut(s) 211, 286, 619
CspAI ACCGGT 1 cut(s) 904
CviAII CATG 4 cut(s) 385, 472, 626, 755
CviQI GTAC 3 cut(s) 211, 286, 619
DdeI CTNAG 4 cut(s) 198, 503, 702, 726
DpnI GATC 4 cut(s) 484, 528, 872, 920
DpnII GATC 4 cut(s) 482, 526, 870, 918
DrdI GACNNNNNNGTC 1 cut(s) 829
DseDI GACNNNNNNGTC 1 cut(s) 829
Eam1104I CTCTTC 1 cut(s) 86
EarI CTCTTC 1 cut(s) 86
EciI GGCGGA 1 cut(s) 630
Eco105I TACGTA 1 cut(s) 210
Eco130I CCWWGG 1 cut(s) 1221
Eco24I GRGCYC 1 cut(s) 615
Eco31I GGTCTC 1 cut(s) 438
Eco47I GGWCC 3 cut(s) 734, 751, 831
Eco57I CTGAAG 3 cut(s) 60, 396, 615
Eco88I CYCGRG 2 cut(s) 614, 1181
EcoRI GAATTC 1 cut(s) 467
EcoT14I CCWWGG 1 cut(s) 1221
EcoT38I GRGCYC 1 cut(s) 615
ErhI CCWWGG 1 cut(s) 1221
FaeI CATG 4 cut(s) 388, 475, 629, 758
FaqI GGGAC 2 cut(s) 192, 790
FatI CATG 4 cut(s) 384, 471, 625, 754
FbaI TGATCA 1 cut(s) 870
FblI GTMKAC 2 cut(s) 575, 840
Fnu4HI GCNGC 4 cut(s) 225, 228, 603, 1119
FokI GGATG 1 cut(s) 595
FriOI GRGCYC 1 cut(s) 615
Fsp4HI GCNGC 4 cut(s) 225, 228, 603, 1119
FspBI CTAG 5 cut(s) 221, 683, 765, 828, 923
GlaI GCGC 1 cut(s) 1270
GluI GCNGC 4 cut(s) 225, 228, 603, 1119
GsaI CCCAGC 1 cut(s) 263
HaeIII GGCC 2 cut(s) 59, 1153
HapII CCGG 4 cut(s) 741, 905, 1085, 1150
HgaI GACGC 1 cut(s) 1038
HhaI GCGC 1 cut(s) 1271
Hin1II CATG 4 cut(s) 388, 475, 629, 758
Hin6I GCGC 1 cut(s) 1269
HinP1I GCGC 1 cut(s) 1269
HindIII AAGCTT 2 cut(s) 666, 704
HinfI GANTC 5 cut(s) 44, 251, 493, 998, 1256
HpaII CCGG 4 cut(s) 741, 905, 1085, 1150
HphI GGTGA 2 cut(s) 488, 1175
Hpy166II GTNNAC 6 cut(s) 28, 286, 576, 794, 841, 941
Hpy188I TCNGA 5 cut(s) 376, 712, 835, 1098, 1131
Hpy188III TCNNGA 8 cut(s) 172, 248, 530, 566, 626, 656, 863, 1233
Hpy8I GTNNAC 6 cut(s) 28, 286, 576, 794, 841, 941
Hpy99I CGWCG 2 cut(s) 35, 1054
HpyCH4III ACNGT 1 cut(s) 986
HpyCH4IV ACGT 2 cut(s) 209, 1251
HpyF10VI GCNNNNNNNGC 5 cut(s) 144, 599, 1055, 1118, 1226
HpyF3I CTNAG 4 cut(s) 198, 503, 702, 726
HpySE526I ACGT 2 cut(s) 209, 1251
Hsp92II CATG 4 cut(s) 388, 475, 629, 758
HspAI GCGC 1 cut(s) 1269
KroI GCCGGC 1 cut(s) 1149
KroNI GCCGGC 1 cut(s) 1151
Ksp22I TGATCA 1 cut(s) 870
Kzo9I GATC 4 cut(s) 482, 526, 870, 918
LmnI GCTCC 3 cut(s) 357, 559, 599
Lsp1109I GCAGC 4 cut(s) 211, 214, 614, 1105
LweI GCATC 1 cut(s) 1238
MaeI CTAG 5 cut(s) 221, 683, 765, 828, 923
MaeII ACGT 2 cut(s) 209, 1251
MaeIII GTNAC 5 cut(s) 293, 757, 973, 980, 1171
MalI GATC 4 cut(s) 484, 528, 872, 920
MbiI CCGCTC 1 cut(s) 1178
MboI GATC 4 cut(s) 482, 526, 870, 918
MboII GAAGA 4 cut(s) 53, 103, 340, 912
MfeI CAATTG 1 cut(s) 1113
MflI RGATCY 3 cut(s) 482, 526, 918
MhlI GDGCHC 3 cut(s) 615, 796, 1188
MluCI AATT 7 cut(s) 151, 315, 467, 517, 535, 692, 1113
MluI ACGCGT 1 cut(s) 1047
MlyI GAGTC 1 cut(s) 1265
MmeI TCCRAC 2 cut(s) 761, 889
MroNI GCCGGC 1 cut(s) 1149
MseI TTAA 3 cut(s) 126, 309, 1029
MspA1I CMGCKG 1 cut(s) 820
MspCI CTTAAG 1 cut(s) 1028
MspI CCGG 4 cut(s) 741, 905, 1085, 1150
MunI CAATTG 1 cut(s) 1113
Mva1269I GAATGC 1 cut(s) 149
MvnI CGCG 4 cut(s) 185, 407, 657, 1049
MwoI GCNNNNNNNGC 5 cut(s) 144, 599, 1055, 1118, 1226
NaeI GCCGGC 1 cut(s) 1151
NdeII GATC 4 cut(s) 482, 526, 870, 918
NgoMIV GCCGGC 1 cut(s) 1149
NlaIII CATG 4 cut(s) 388, 475, 629, 758
NlaIV GGNNCC 4 cut(s) 528, 561, 1187, 1219
NmuCI GTSAC 2 cut(s) 757, 1171
NruI TCGCGA 1 cut(s) 657
NspV TTCGAA 1 cut(s) 551
PaeR7I CTCGAG 1 cut(s) 614
PagI TCATGA 1 cut(s) 625
PcsI WCGNNNNNNNCGW 2 cut(s) 182, 411
PctI GAATGC 1 cut(s) 149
PdiI GCCGGC 1 cut(s) 1151
PfeI GAWTC 4 cut(s) 44, 251, 493, 998
Pfl23II CGTACG 1 cut(s) 210
PinAI ACCGGT 1 cut(s) 904
PkrI GCNGC 4 cut(s) 226, 229, 604, 1120
PleI GAGTC 1 cut(s) 1264
PpsI GAGTC 1 cut(s) 1264
Ppu21I YACGTR 1 cut(s) 210
PspFI CCCAGC 1 cut(s) 259
PspLI CGTACG 1 cut(s) 210
PspN4I GGNNCC 4 cut(s) 528, 561, 1187, 1219
PspPI GGNCC 4 cut(s) 57, 734, 751, 831
PspXI VCTCGAGB 1 cut(s) 614
PsuI RGATCY 3 cut(s) 482, 526, 918
PvuII CAGCTG 1 cut(s) 820
RruI TCGCGA 1 cut(s) 657
RsaI GTAC 3 cut(s) 212, 287, 620
RsaNI GTAC 3 cut(s) 211, 286, 619
SaqAI TTAA 3 cut(s) 126, 309, 1029
SatI GCNGC 4 cut(s) 225, 228, 603, 1119
Sau3AI GATC 4 cut(s) 482, 526, 870, 918
Sau96I GGNCC 4 cut(s) 57, 734, 751, 831
SchI GAGTC 1 cut(s) 1265
SduI GDGCHC 3 cut(s) 615, 796, 1188
SfaNI GCATC 1 cut(s) 1238
SfcI CTRYAG 2 cut(s) 841, 1289
Sfr274I CTCGAG 1 cut(s) 614
SfuI TTCGAA 1 cut(s) 551
SinI GGWCC 3 cut(s) 734, 751, 831
SlaI CTCGAG 1 cut(s) 614
SmlI CTYRAG 5 cut(s) 79, 170, 614, 863, 1028
SmoI CTYRAG 5 cut(s) 79, 170, 614, 863, 1028
SnaBI TACGTA 1 cut(s) 210
Sse9I AATT 7 cut(s) 151, 315, 467, 517, 535, 692, 1113
SsiI CCGC 2 cut(s) 641, 1176
SspMI CTAG 5 cut(s) 221, 683, 765, 828, 923
StyI CCWWGG 1 cut(s) 1221
TaaI ACNGT 1 cut(s) 986
TaiI ACGT 2 cut(s) 212, 1254
TaqI TCGA 7 cut(s) 551, 615, 996, 1052, 1072, 1157, 1232
TasI AATT 7 cut(s) 151, 315, 467, 517, 535, 692, 1113
TatI WGTACW 1 cut(s) 285
TfiI GAWTC 4 cut(s) 44, 251, 493, 998
Tru1I TTAA 3 cut(s) 126, 309, 1029
Tru9I TTAA 3 cut(s) 126, 309, 1029
TscAI CASTG 2 cut(s) 330, 651
TseFI GTSAC 2 cut(s) 757, 1171
TseI GCWGC 4 cut(s) 224, 227, 602, 1118
Tsp45I GTSAC 2 cut(s) 757, 1171
TspDTI ATGAA 5 cut(s) 460, 480, 599, 614, 936
TspGWI ACGGA 1 cut(s) 895
TspRI CASTG 2 cut(s) 330, 651
Vha464I CTTAAG 1 cut(s) 1028
VneI GTGCAC 1 cut(s) 792
VpaK11BI GGWCC 3 cut(s) 734, 751, 831
XapI RAATTY 4 cut(s) 151, 467, 535, 692
XhoI CTCGAG 1 cut(s) 614
XmiI GTMKAC 2 cut(s) 575, 840
XspI CTAG 5 cut(s) 221, 683, 765, 828, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.