Prupe.4G023800_v2.0.a1
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0015787
Physical Location & Seq
Forward (+)
1128122 .. 1130416
2295 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G023800.1

Sequence Viewer

Length: 1266 bp
ATGGACTATTTGAGAGAGTTTGCAGAAAAAGTAACGTTTCTGCCGCCGGAGGCAGAGGCGCCAGGGAGGAGAGAAAAGATGAGCTGCTTCTCTTGTTGCAAGCGGGTGGATGAGCCTGAAGAATCTGTAAGGAATACCAGAACAGGGAGGACATATTCTAATGCTAGTGGCGGGAGGAGATTGACCGCCCTTATTGCAACTTTCTCTATTAAGTCAGGTAGCAGCCAATGTAGGAATTTGCACAAAGAGATACTCAAGACTGGACATGCAAAAAAATCTGCTCAGCTCTTTACATATCAGGAATTAGCTGCTGCAACTGATAACTTCAATCCTGATTCTCTGGTGGGAGAAGGTGGATTTGGGAAGGTGTACAAAGGACACATAGACAGCATCAAACAAGTTGTGGCCGTGAAGCAACTGGACCGGAAAGGATTGCAAGGGGGTAGAGAGTTCTGTTCTGAAGTTATCATGTTGAGTTTGGTTCAACACCCAAACCTCGTCAATCTGATTGGCTACTGTGCAGAAGGAGACCAGAAAATTTTAGTGTATGAATTCATGGCCAATGGTTCTTTGGAGAATCACTTTCTAGATCTATCTCCAAATAGAGAGCCTCTTGATTGGTATACCAGGATGAAAATAGCCGAAGGAGCAGCTAGAGGACTTGAGTACCTGCATGAGACTGCCAATCCACCGGTGATTTATCGCGACTTCAAAGCATCTAATATACTGTTAGATGACAAATTCAGTCCTAAGCTCTCTGATTTTGGACTTGCTAAGTTAGGTCCAACCGGAGACAAGGACCATGTGTCTACAAGGGTGATGGGAACTTATGGCTATTGTGCACCAGAGTATGCTTCAACAGGACAGCTGACAACCATGTCTGATGTATACAGCTTCGGCGTTGTATTTCTGGAGTTAATCACGGAAAGGAGAGCTATTGATGATAGAAGGCCAGTTAGAGAACAGAACCTAGTCAGTTGGGCAAAGCCAATGTTAATGGACAGAATAAACTATGCATCAATGGCTGACCCGTTGCTTGGAGGGAAATTTCCGGTAAAGGGTTTGAATCAAGCTGTTGCAATTGCAGCAATGTGTCTCAATGAGGAAGCTGACTGCCGACCTTCGATGGGTGATGTTGTAACTGCCCTTGAGCATCTATCCTTGCCAATATATGATGAACTGGATGCCAAGGGTGCATCAGGAAACAATGGTGGGCATGTTGAATCAGTAAAAAGAAAAAGTTTTAAAGGAGACCGAGAGCTATAG

Protein Analysis

422

Amino Acids

46.63

Weight (kDa)

6.53

Isoelectric Point (pI)

35.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015787)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61860
fragaria_vesca FvH4_3g02440
malus_domestica MD05G1343000.v1.1 MD10G1318000.v1.1
prunus_persica Prupe.4G023800_v2.0.a1
pyrus_communis pycom05g31250
rosa_chinensis RchiOBHm_Chr5g0003691
rosa_laevigata RLG00000031166
rosa_multiflora Rmu_sc0000500.1_g000001
rosa_rugosa Rorug04G0405900
rosa_samantha Rh5AG032700 Rh5BG031300 Rh5CG035400 Rh5DG031900
rosa_wichuraiana Rw5G003030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 877
Acc36I ACCTGC 1 cut(s) 678
AccB1I GGYRCC 1 cut(s) 58
AccI GTMKAC 3 cut(s) 623, 809, 888
AccII CGCG 1 cut(s) 705
AciI CCGC 4 cut(s) 44, 103, 171, 186
AclI AACGTT 1 cut(s) 35
AcoI YGGCCR 2 cut(s) 405, 558
AcsI RAATTY 5 cut(s) 235, 537, 551, 740, 1046
AcuI CTGAAG 2 cut(s) 138, 480
AcyI GRCGYC 1 cut(s) 59
AfaI GTAC 2 cut(s) 371, 668
AfiI CCNNNNNNNGG 4 cut(s) 144, 1037, 1058, 1127
AgeI ACCGGT 1 cut(s) 691
AgsI TTSAA 6 cut(s) 328, 485, 712, 858, 1066, 1223
AhdI GACNNNNNGTC 1 cut(s) 805
AjnI CCWGG 2 cut(s) 61, 626
AjuI GAANNNNNNNTTGG 2 cut(s) 342, 374
Alw21I GWGCWC 1 cut(s) 844
Alw26I GTCTC 5 cut(s) 522, 671, 786, 1100, 1245
Alw44I GTGCAC 1 cut(s) 840
AoxI GGCC 3 cut(s) 405, 558, 950
ApaLI GTGCAC 1 cut(s) 840
ApeKI GCWGC 6 cut(s) 84, 222, 308, 311, 650, 1085
ApoI RAATTY 5 cut(s) 235, 537, 551, 740, 1046
AsiGI ACCGGT 1 cut(s) 691
AspLEI GCGC 1 cut(s) 61
AspS9I GGNCC 3 cut(s) 421, 782, 799
AsuHPI GGTGA 3 cut(s) 706, 829, 1142
AvaII GGWCC 3 cut(s) 421, 782, 799
BaeGI GKGCMC 1 cut(s) 844
BaeI ACNNNNGTAYC 2 cut(s) 650, 683
BalI TGGCCA 1 cut(s) 560
BanI GGYRCC 1 cut(s) 58
Bbv12I GWGCWC 1 cut(s) 844
BbvI GCAGC 6 cut(s) 71, 234, 295, 298, 662, 1097
BccI CCATC 2 cut(s) 814, 1120
BceAI ACGGC 1 cut(s) 392
BciT130I CCWGG 2 cut(s) 63, 628
BcoDI GTCTC 5 cut(s) 522, 671, 786, 1100, 1245
BfaI CTAG 4 cut(s) 165, 587, 654, 971
BfmI CTRYAG 1 cut(s) 1262
BfoI RGCGCY 1 cut(s) 62
BfuAI ACCTGC 1 cut(s) 678
BglII AGATCT 1 cut(s) 589
BisI GCNGC 7 cut(s) 44, 85, 223, 309, 312, 651, 1086
BlpI GCTNAGC 1 cut(s) 282
BlsI GCNGC 7 cut(s) 45, 86, 224, 310, 313, 652, 1087
Bme1390I CCNGG 2 cut(s) 63, 628
Bme18I GGWCC 3 cut(s) 421, 782, 799
BmeRI GACNNNNNGTC 1 cut(s) 805
BmgT120I GGNCC 3 cut(s) 421, 782, 799
BmiI GGNNCC 1 cut(s) 60
BmrFI CCNGG 2 cut(s) 63, 628
BmsI GCATC 6 cut(s) 399, 725, 1025, 1162, 1174, 1205
BpmI CTGGAG 1 cut(s) 932
Bpu10I CCTNAGC 1 cut(s) 750
Bpu1102I GCTNAGC 1 cut(s) 282
BpuEI CTTGAG 3 cut(s) 239, 683, 1169
BsaHI GRCGYC 1 cut(s) 59
BsaI GGTCTC 2 cut(s) 522, 1245
BsaJI CCNNGG 2 cut(s) 62, 1188
BsaWI WCCGGW 4 cut(s) 423, 691, 788, 1051
Bsc4I CCNNNNNNNGG 4 cut(s) 144, 1037, 1058, 1127
Bse118I RCCGGY 1 cut(s) 691
Bse1I ACTGG 4 cut(s) 265, 423, 953, 1185
Bse3DI GCAATG 1 cut(s) 1095
BseBI CCWGG 2 cut(s) 63, 628
BseDI CCNNGG 2 cut(s) 62, 1188
BseGI GGATG 3 cut(s) 115, 636, 1189
BseLI CCNNNNNNNGG 4 cut(s) 144, 1037, 1058, 1127
BseMI GCAATG 1 cut(s) 1095
BseMII CTCAG 1 cut(s) 296
BseNI ACTGG 4 cut(s) 265, 423, 953, 1185
BseRI GAGGAG 2 cut(s) 82, 190
BseSI GKGCMC 1 cut(s) 844
BseXI GCAGC 6 cut(s) 71, 234, 295, 298, 662, 1097
BsgI GTGCAG 1 cut(s) 540
Bsh1236I CGCG 1 cut(s) 705
BshFI GGCC 3 cut(s) 407, 560, 952
BshNI GGYRCC 1 cut(s) 58
BshTI ACCGGT 1 cut(s) 691
BsiHKAI GWGCWC 1 cut(s) 844
BsiSI CCGG 5 cut(s) 47, 424, 692, 789, 1052
BslI CCNNNNNNNGG 4 cut(s) 144, 1037, 1058, 1127
BsmAI GTCTC 5 cut(s) 522, 671, 786, 1100, 1245
BsnI GGCC 3 cut(s) 407, 560, 952
Bso31I GGTCTC 2 cut(s) 522, 1245
Bsp1286I GDGCHC 1 cut(s) 844
Bsp1407I TGTACA 1 cut(s) 369
Bsp143I GATC 1 cut(s) 589
Bsp1720I GCTNAGC 1 cut(s) 282
Bsp68I TCGCGA 1 cut(s) 705
BspACI CCGC 4 cut(s) 44, 103, 171, 186
BspANI GGCC 3 cut(s) 407, 560, 952
BspCNI CTCAG 1 cut(s) 295
BspFNI CGCG 1 cut(s) 705
BspLI GGNNCC 1 cut(s) 60
BspMI ACCTGC 1 cut(s) 678
BspT107I GGYRCC 1 cut(s) 58
BspTNI GGTCTC 2 cut(s) 522, 1245
BsrDI GCAATG 1 cut(s) 1095
BsrFI RCCGGY 1 cut(s) 691
BsrGI TGTACA 1 cut(s) 369
BsrI ACTGG 4 cut(s) 265, 423, 953, 1185
BssAI RCCGGY 1 cut(s) 691
BssECI CCNNGG 2 cut(s) 62, 1188
BssMI GATC 1 cut(s) 589
BssNAI GTATAC 2 cut(s) 624, 889
BssNI GRCGYC 1 cut(s) 59
BssT1I CCWWGG 1 cut(s) 1188
Bst1107I GTATAC 2 cut(s) 624, 889
Bst2UI CCWGG 2 cut(s) 63, 628
Bst4CI ACNGT 2 cut(s) 518, 729
BstACI GRCGYC 1 cut(s) 59
BstAUI TGTACA 1 cut(s) 369
BstC8I GCNNGC 1 cut(s) 101
BstDEI CTNAG 3 cut(s) 282, 750, 774
BstF5I GGATG 3 cut(s) 115, 636, 1189
BstFNI CGCG 1 cut(s) 705
BstH2I RGCGCY 1 cut(s) 62
BstHHI GCGC 1 cut(s) 61
BstKTI GATC 1 cut(s) 592
BstMAI GTCTC 5 cut(s) 522, 671, 786, 1100, 1245
BstMBI GATC 1 cut(s) 589
BstMWI GCNNNNNNNGC 5 cut(s) 194, 647, 1022, 1085, 1193
BstNI CCWGG 2 cut(s) 63, 628
BstNSI RCATGY 2 cut(s) 269, 1220
BstSCI CCNGG 2 cut(s) 61, 626
BstSFI CTRYAG 1 cut(s) 1262
BstSLI GKGCMC 1 cut(s) 844
BstUI CGCG 1 cut(s) 705
BstV1I GCAGC 6 cut(s) 71, 234, 295, 298, 662, 1097
BstX2I RGATCY 1 cut(s) 589
BstYI RGATCY 1 cut(s) 589
BstZ17I GTATAC 2 cut(s) 624, 889
BsuRI GGCC 3 cut(s) 407, 560, 952
BtsCI GGATG 3 cut(s) 115, 636, 1189
BtuMI TCGCGA 1 cut(s) 705
BveI ACCTGC 1 cut(s) 678
Cac8I GCNNGC 1 cut(s) 101
CfoI GCGC 1 cut(s) 61
Cfr10I RCCGGY 1 cut(s) 691
Cfr13I GGNCC 3 cut(s) 421, 782, 799
Csp6I GTAC 2 cut(s) 370, 667
CspAI ACCGGT 1 cut(s) 691
CviAII CATG 7 cut(s) 266, 469, 556, 674, 803, 877, 1217
CviQI GTAC 2 cut(s) 370, 667
DdeI CTNAG 3 cut(s) 282, 750, 774
DinI GGCGCC 1 cut(s) 60
DpnI GATC 1 cut(s) 591
DpnII GATC 1 cut(s) 589
DraI TTTAAA 1 cut(s) 1246
DrdI GACNNNNNNGTC 1 cut(s) 877
DriI GACNNNNNGTC 1 cut(s) 805
DseDI GACNNNNNNGTC 1 cut(s) 877
EaeI YGGCCR 2 cut(s) 405, 558
Eam1105I GACNNNNNGTC 1 cut(s) 805
Eco130I CCWWGG 1 cut(s) 1188
Eco31I GGTCTC 2 cut(s) 522, 1245
Eco47I GGWCC 3 cut(s) 421, 782, 799
Eco57I CTGAAG 2 cut(s) 138, 480
EcoRI GAATTC 1 cut(s) 551
EcoRII CCWGG 2 cut(s) 61, 626
EcoT14I CCWWGG 1 cut(s) 1188
EcoT22I ATGCAT 1 cut(s) 1018
EgeI GGCGCC 1 cut(s) 60
EheI GGCGCC 1 cut(s) 60
ErhI CCWWGG 1 cut(s) 1188
FaeI CATG 7 cut(s) 269, 472, 559, 677, 806, 880, 1220
FatI CATG 7 cut(s) 265, 468, 555, 673, 802, 876, 1216
FauI CCCGC 2 cut(s) 96, 164
FblI GTMKAC 3 cut(s) 623, 809, 888
Fnu4HI GCNGC 7 cut(s) 44, 85, 223, 309, 312, 651, 1086
FokI GGATG 3 cut(s) 122, 643, 1196
Fsp4HI GCNGC 7 cut(s) 44, 85, 223, 309, 312, 651, 1086
FspBI CTAG 4 cut(s) 165, 587, 654, 971
GlaI GCGC 1 cut(s) 60
GluI GCNGC 7 cut(s) 44, 85, 223, 309, 312, 651, 1086
GsuI CTGGAG 1 cut(s) 932
HaeII RGCGCY 1 cut(s) 62
HaeIII GGCC 3 cut(s) 407, 560, 952
HapII CCGG 5 cut(s) 47, 424, 692, 789, 1052
HhaI GCGC 1 cut(s) 61
Hin1I GRCGYC 1 cut(s) 59
Hin1II CATG 7 cut(s) 269, 472, 559, 677, 806, 880, 1220
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HinfI GANTC 5 cut(s) 122, 335, 577, 1066, 1223
HpaII CCGG 5 cut(s) 47, 424, 692, 789, 1052
HphI GGTGA 3 cut(s) 706, 829, 1142
Hpy166II GTNNAC 5 cut(s) 370, 624, 810, 842, 889
Hpy188I TCNGA 4 cut(s) 460, 507, 760, 883
Hpy188III TCNNGA 8 cut(s) 256, 299, 332, 587, 614, 704, 911, 1200
Hpy8I GTNNAC 5 cut(s) 370, 624, 810, 842, 889
HpyAV CCTTC 6 cut(s) 344, 358, 518, 638, 942, 1131
HpyCH4III ACNGT 2 cut(s) 518, 729
HpyCH4IV ACGT 1 cut(s) 35
HpyF10VI GCNNNNNNNGC 5 cut(s) 194, 647, 1022, 1085, 1193
HpyF3I CTNAG 3 cut(s) 282, 750, 774
HpySE526I ACGT 1 cut(s) 35
Hsp92I GRCGYC 1 cut(s) 59
Hsp92II CATG 7 cut(s) 269, 472, 559, 677, 806, 880, 1220
HspAI GCGC 1 cut(s) 59
KasI GGCGCC 1 cut(s) 58
Kzo9I GATC 1 cut(s) 589
LmnI GCTCC 1 cut(s) 647
Lsp1109I GCAGC 6 cut(s) 71, 234, 295, 298, 662, 1097
LweI GCATC 6 cut(s) 399, 725, 1025, 1162, 1174, 1205
MaeI CTAG 4 cut(s) 165, 587, 654, 971
MaeII ACGT 1 cut(s) 35
MaeIII GTNAC 2 cut(s) 31, 1138
MalI GATC 1 cut(s) 591
MboI GATC 1 cut(s) 589
MboII GAAGA 1 cut(s) 131
MfeI CAATTG 1 cut(s) 1080
MflI RGATCY 1 cut(s) 589
MhlI GDGCHC 1 cut(s) 844
MlsI TGGCCA 1 cut(s) 560
MluCI AATT 7 cut(s) 235, 302, 537, 551, 740, 1046, 1080
MluNI TGGCCA 1 cut(s) 560
Mly113I GGCGCC 1 cut(s) 59
MmeI TCCRAC 1 cut(s) 809
Mox20I TGGCCA 1 cut(s) 560
Mph1103I ATGCAT 1 cut(s) 1018
MscI TGGCCA 1 cut(s) 560
MseI TTAA 4 cut(s) 210, 917, 995, 1245
Msp20I TGGCCA 1 cut(s) 560
MspA1I CMGCKG 1 cut(s) 868
MspI CCGG 5 cut(s) 47, 424, 692, 789, 1052
MspR9I CCNGG 2 cut(s) 63, 628
MunI CAATTG 1 cut(s) 1080
MvaI CCWGG 2 cut(s) 63, 628
MvnI CGCG 1 cut(s) 705
MwoI GCNNNNNNNGC 5 cut(s) 194, 647, 1022, 1085, 1193
NarI GGCGCC 1 cut(s) 59
NdeII GATC 1 cut(s) 589
NlaIII CATG 7 cut(s) 269, 472, 559, 677, 806, 880, 1220
NlaIV GGNNCC 1 cut(s) 60
NruI TCGCGA 1 cut(s) 705
NsiI ATGCAT 1 cut(s) 1018
NspI RCATGY 2 cut(s) 269, 1220
PfeI GAWTC 5 cut(s) 122, 335, 577, 1066, 1223
PinAI ACCGGT 1 cut(s) 691
PkrI GCNGC 7 cut(s) 45, 86, 224, 310, 313, 652, 1087
PluTI GGCGCC 1 cut(s) 62
Psp1406I AACGTT 1 cut(s) 35
Psp6I CCWGG 2 cut(s) 61, 626
PspGI CCWGG 2 cut(s) 61, 626
PspN4I GGNNCC 1 cut(s) 60
PspPI GGNCC 3 cut(s) 421, 782, 799
PsuI RGATCY 1 cut(s) 589
PvuII CAGCTG 1 cut(s) 868
RruI TCGCGA 1 cut(s) 705
RsaI GTAC 2 cut(s) 371, 668
RsaNI GTAC 2 cut(s) 370, 667
SaqAI TTAA 4 cut(s) 210, 917, 995, 1245
SatI GCNGC 7 cut(s) 44, 85, 223, 309, 312, 651, 1086
Sau3AI GATC 1 cut(s) 589
Sau96I GGNCC 3 cut(s) 421, 782, 799
ScrFI CCNGG 2 cut(s) 63, 628
SduI GDGCHC 1 cut(s) 844
SfaNI GCATC 6 cut(s) 399, 725, 1025, 1162, 1174, 1205
SfcI CTRYAG 1 cut(s) 1262
SfoI GGCGCC 1 cut(s) 60
SgrAI CRCCGGYG 1 cut(s) 691
SinI GGWCC 3 cut(s) 421, 782, 799
SmlI CTYRAG 3 cut(s) 254, 662, 1148
SmoI CTYRAG 3 cut(s) 254, 662, 1148
Sse9I AATT 7 cut(s) 235, 302, 537, 551, 740, 1046, 1080
SsiI CCGC 4 cut(s) 44, 103, 171, 186
SspDI GGCGCC 1 cut(s) 58
SspMI CTAG 4 cut(s) 165, 587, 654, 971
StyD4I CCNGG 2 cut(s) 61, 626
StyI CCWWGG 1 cut(s) 1188
TaaI ACNGT 2 cut(s) 518, 729
TaiI ACGT 1 cut(s) 38
TaqI TCGA 1 cut(s) 1124
TasI AATT 7 cut(s) 235, 302, 537, 551, 740, 1046, 1080
TatI WGTACW 1 cut(s) 369
TauI GCSGC 1 cut(s) 46
TfiI GAWTC 5 cut(s) 122, 335, 577, 1066, 1223
Tru1I TTAA 4 cut(s) 210, 917, 995, 1245
Tru9I TTAA 4 cut(s) 210, 917, 995, 1245
TseI GCWGC 6 cut(s) 84, 222, 308, 311, 650, 1085
TspDTI ATGAA 4 cut(s) 544, 564, 647, 1191
TspGWI ACGGA 1 cut(s) 938
VneI GTGCAC 1 cut(s) 840
VpaK11BI GGWCC 3 cut(s) 421, 782, 799
XapI RAATTY 5 cut(s) 235, 537, 551, 740, 1046
XbaI TCTAGA 1 cut(s) 586
XceI RCATGY 2 cut(s) 269, 1220
XcmI CCANNNNNNNNNTGG 1 cut(s) 568
XmiI GTMKAC 3 cut(s) 623, 809, 888
XspI CTAG 4 cut(s) 165, 587, 654, 971
Zsp2I ATGCAT 1 cut(s) 1018
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.