RLG00000031166
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0015787
Physical Location & Seq
Forward (+)
2648545 .. 2650253
1709 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031166

Sequence Viewer

Length: 1197 bp
ATGCGCTGCCTCTCTTGTTGCAAGCCGGTGGAGGATGATCCTCCGAAATTTGTAGTCAGAAAGACAGGCCGAAGGCCGTATTCTAATACTGCCACGCCATTGGACTCCTTTTTCTCAACGCTCTCCCTTAAGTCAGGCAGCAGCAGATGCAGGAATTTGCGCAATCAGATACTCAAGGTGGGAAGTGCAAAGCAATCTGCTCGGCTTTTTACGTTCAATGAGCTTCATGCTGCAACTGAGGGCTTCAAGGCTGCTTGTTTGCTTGGCGAAGGAGGATTTGGGAAGGTGTACAAAGGGTACATAGATAGCATCAATTCTAAACAGGCTGTGGCGGTTAAGAAACTTAACAGGAAAGGAATGCAAGGAAGTAGAGAGTTTTGTGCTGAAGTTATCATGTTGAGTCTGTTTCAACACCCAAACCTTGTCAGTCTAGTAGGGTACTGTGCGGAAGGAGAGCAGAGAGTTTTAGTGTATGAATATATGGCCAGTGGATCTTTGGAGGATCACCTTCTAGACATAGGTCCGAATCAGCAGCCTCTTGATTGGTACACTAGAATGACAATAGCTGAAGGAGCAGCTAAAGGACTTGAGTACTTGCATGATGTTGCCAAACCGGCAGTGATTTATCGCGATTTCAAAGCATCAAACATATTGTTGGATTCCCAATTCACTCCGAAGCTGTCTGATTTTGGACTTGCTAAGTTAGGTCCAACAGGAGACAAGTCCCATGTGTCTACAAGGGTGATGGGAACTTATGGCTATTGTGCCCCAGAGTATGCTTGCACAGGAAAACTCACAAAAATGTCTGATGTCTACAGTTTCGGGGTTGTCTTTCTTGAGTTAATCACCGGAAGAAGAGCTGTTGATGAGACAAAACCAGAGGAAGAGCAGATCCTAGTCAGTTGGGCACAACCACTGTTGAAGGATAGAAGTAAATTTTCATTAATTGCTGATCCGTTGCTTAGGGGAAACTTTCCGGTGAAGGGTCTGCATCAAGCTCTTGCAATTGCAGCAATGTGTCTCAATGAGGAAGCTGATTGCCGACCTGTTATGTCTGATGTTGTACTTGCTCTTGAGCATTTATCCAAGCCAAGAAATGAAGTTGTCATTGATGATGAATCAAAACACAGCCCTGTGCAGCTGACCAATGTCCAATCTCTAAAACGAAGAAGTTCCAAAGCAGAGCTAGATCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

399

Amino Acids

44.04

Weight (kDa)

8.63

Isoelectric Point (pI)

39.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 86 - 295 6.3e-45 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 87 - 297 7.4e-42 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015787)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61860
fragaria_vesca FvH4_3g02440
malus_domestica MD05G1343000.v1.1 MD10G1318000.v1.1
prunus_persica Prupe.4G023800_v2.0.a1
pyrus_communis pycom05g31250
rosa_chinensis RchiOBHm_Chr5g0003691
rosa_laevigata RLG00000031166
rosa_multiflora Rmu_sc0000500.1_g000001
rosa_rugosa Rorug04G0405900
rosa_samantha Rh5AG032700 Rh5BG031300 Rh5CG035400 Rh5DG031900
rosa_wichuraiana Rw5G003030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 161
AccI GTMKAC 2 cut(s) 734, 813
AccII CGCG 1 cut(s) 630
AciI CCGC 2 cut(s) 332, 446
AclWI GGATC 5 cut(s) 32, 499, 510, 886, 947
AcoI YGGCCR 1 cut(s) 483
AcsI RAATTY 3 cut(s) 47, 154, 935
AcuI CTGAAG 2 cut(s) 405, 588
AfaI GTAC 6 cut(s) 290, 299, 440, 548, 593, 1065
AfiI CCNNNNNNNGG 1 cut(s) 983
AflII CTTAAG 1 cut(s) 128
AgsI TTSAA 5 cut(s) 217, 247, 410, 637, 922
AjuI GAANNNNNNNTTGG 2 cut(s) 261, 293
AluBI AGCT 9 cut(s) 223, 566, 578, 679, 860, 998, 1034, 1141, 1186
AluI AGCT 9 cut(s) 223, 566, 578, 679, 860, 998, 1034, 1141, 1186
Alw26I GTCTC 3 cut(s) 711, 863, 1025
AlwI GGATC 5 cut(s) 32, 499, 510, 886, 947
AoxI GGCC 3 cut(s) 67, 74, 483
ApeKI GCWGC 9 cut(s) 6, 138, 141, 230, 251, 532, 575, 1010, 1138
ApoI RAATTY 3 cut(s) 47, 154, 935
AseI ATTAAT 1 cut(s) 944
Asp700I GAANNNNTTC 1 cut(s) 1171
AspLEI GCGC 2 cut(s) 6, 162
AspS9I GGNCC 2 cut(s) 521, 707
AsuHPI GGTGA 4 cut(s) 497, 754, 838, 991
AvaII GGWCC 2 cut(s) 521, 707
BaeGI GKGCMC 2 cut(s) 769, 910
BalI TGGCCA 1 cut(s) 485
BbvI GCAGC 8 cut(s) 150, 153, 217, 238, 544, 587, 1022, 1150
BccI CCATC 1 cut(s) 739
BceAI ACGGC 1 cut(s) 61
BcgI CGANNNNNNTGC 2 cut(s) 182, 216
BcoDI GTCTC 3 cut(s) 711, 863, 1025
BfaI CTAG 5 cut(s) 431, 512, 552, 896, 1187
BfmI CTRYAG 2 cut(s) 814, 1193
BfrI CTTAAG 1 cut(s) 128
BglI GCCNNNNNGGC 1 cut(s) 614
BglII AGATCT 1 cut(s) 1189
BisI GCNGC 9 cut(s) 7, 139, 142, 231, 252, 533, 576, 1011, 1139
BlsI GCNGC 9 cut(s) 8, 140, 143, 232, 253, 534, 577, 1012, 1140
BmcAI AGTACT 1 cut(s) 593
Bme18I GGWCC 2 cut(s) 521, 707
BmgT120I GGNCC 2 cut(s) 521, 707
BmsI GCATC 4 cut(s) 137, 318, 650, 1000
BoxI GACNNNNGTC 2 cut(s) 519, 1148
Bpu10I CCTNAGC 1 cut(s) 962
BpuEI CTTGAG 4 cut(s) 158, 608, 857, 1094
BsaWI WCCGGW 2 cut(s) 848, 976
Bsc4I CCNNNNNNNGG 1 cut(s) 983
Bse118I RCCGGY 2 cut(s) 25, 613
Bse1I ACTGG 1 cut(s) 486
Bse3DI GCAATG 1 cut(s) 1020
BseGI GGATG 1 cut(s) 40
BseLI CCNNNNNNNGG 1 cut(s) 983
BseMI GCAATG 1 cut(s) 1020
BseMII CTCAG 1 cut(s) 228
BseNI ACTGG 1 cut(s) 486
BseSI GKGCMC 2 cut(s) 769, 910
BseXI GCAGC 8 cut(s) 150, 153, 217, 238, 544, 587, 1022, 1150
BsgI GTGCAG 1 cut(s) 1157
Bsh1236I CGCG 1 cut(s) 630
BshFI GGCC 3 cut(s) 69, 76, 485
BsiSI CCGG 4 cut(s) 26, 614, 849, 977
BslFI GGGAC 1 cut(s) 709
BslI CCNNNNNNNGG 1 cut(s) 983
BsmAI GTCTC 3 cut(s) 711, 863, 1025
BsmFI GGGAC 1 cut(s) 709
BsmI GAATGC 1 cut(s) 363
BsnI GGCC 3 cut(s) 69, 76, 485
Bsp1286I GDGCHC 2 cut(s) 769, 910
Bsp1407I TGTACA 1 cut(s) 288
Bsp143I GATC 6 cut(s) 37, 491, 502, 891, 952, 1189
Bsp68I TCGCGA 1 cut(s) 630
BspACI CCGC 2 cut(s) 332, 446
BspANI GGCC 3 cut(s) 69, 76, 485
BspCNI CTCAG 1 cut(s) 229
BspFNI CGCG 1 cut(s) 630
BspPI GGATC 5 cut(s) 32, 499, 510, 886, 947
BspQI GCTCTTC 2 cut(s) 850, 879
BspTI CTTAAG 1 cut(s) 128
BsrDI GCAATG 1 cut(s) 1020
BsrFI RCCGGY 2 cut(s) 25, 613
BsrGI TGTACA 1 cut(s) 288
BsrI ACTGG 1 cut(s) 486
BssAI RCCGGY 2 cut(s) 25, 613
BssMI GATC 6 cut(s) 37, 491, 502, 891, 952, 1189
Bst4CI ACNGT 3 cut(s) 443, 818, 918
Bst6I CTCTTC 2 cut(s) 850, 879
BstAFI CTTAAG 1 cut(s) 128
BstAPI GCANNNNNTGC 1 cut(s) 147
BstAUI TGTACA 1 cut(s) 288
BstC8I GCNNGC 2 cut(s) 23, 781
BstDEI CTNAG 3 cut(s) 237, 699, 962
BstF5I GGATG 1 cut(s) 40
BstFNI CGCG 1 cut(s) 630
BstHHI GCGC 2 cut(s) 6, 162
BstKTI GATC 6 cut(s) 40, 494, 505, 894, 955, 1192
BstMAI GTCTC 3 cut(s) 711, 863, 1025
BstMBI GATC 6 cut(s) 37, 491, 502, 891, 952, 1189
BstMWI GCNNNNNNNGC 4 cut(s) 147, 572, 614, 1010
BstPAI GACNNNNGTC 2 cut(s) 519, 1148
BstSFI CTRYAG 2 cut(s) 814, 1193
BstSLI GKGCMC 2 cut(s) 769, 910
BstUI CGCG 1 cut(s) 630
BstV1I GCAGC 8 cut(s) 150, 153, 217, 238, 544, 587, 1022, 1150
BstX2I RGATCY 3 cut(s) 491, 891, 1189
BstXI CCANNNNNNTGG 1 cut(s) 100
BstYI RGATCY 3 cut(s) 491, 891, 1189
BsuRI GGCC 3 cut(s) 69, 76, 485
BtsCI GGATG 1 cut(s) 40
BtsI GCAGTG 1 cut(s) 624
BtsIMutI CAGTG 3 cut(s) 493, 624, 914
BtuMI TCGCGA 1 cut(s) 630
Cac8I GCNNGC 2 cut(s) 23, 781
CfoI GCGC 2 cut(s) 6, 162
Cfr10I RCCGGY 2 cut(s) 25, 613
Cfr13I GGNCC 2 cut(s) 521, 707
Csp6I GTAC 6 cut(s) 289, 298, 439, 547, 592, 1064
CviAII CATG 4 cut(s) 227, 394, 599, 728
CviQI GTAC 6 cut(s) 289, 298, 439, 547, 592, 1064
DdeI CTNAG 3 cut(s) 237, 699, 962
DpnI GATC 6 cut(s) 39, 493, 504, 893, 954, 1191
DpnII GATC 6 cut(s) 37, 491, 502, 891, 952, 1189
EaeI YGGCCR 1 cut(s) 483
Eam1104I CTCTTC 2 cut(s) 850, 879
EarI CTCTTC 2 cut(s) 850, 879
Eco47I GGWCC 2 cut(s) 521, 707
Eco57I CTGAAG 2 cut(s) 405, 588
FaeI CATG 4 cut(s) 230, 397, 602, 731
FaqI GGGAC 1 cut(s) 709
FatI CATG 4 cut(s) 226, 393, 598, 727
FblI GTMKAC 2 cut(s) 734, 813
Fnu4HI GCNGC 9 cut(s) 7, 139, 142, 231, 252, 533, 576, 1011, 1139
FokI GGATG 1 cut(s) 47
Fsp4HI GCNGC 9 cut(s) 7, 139, 142, 231, 252, 533, 576, 1011, 1139
FspBI CTAG 5 cut(s) 431, 512, 552, 896, 1187
FspI TGCGCA 1 cut(s) 161
GlaI GCGC 2 cut(s) 5, 161
GluI GCNGC 9 cut(s) 7, 139, 142, 231, 252, 533, 576, 1011, 1139
HaeIII GGCC 3 cut(s) 69, 76, 485
HapII CCGG 4 cut(s) 26, 614, 849, 977
HhaI GCGC 2 cut(s) 6, 162
Hin1II CATG 4 cut(s) 230, 397, 602, 731
Hin6I GCGC 2 cut(s) 4, 160
HinP1I GCGC 2 cut(s) 4, 160
HinfI GANTC 5 cut(s) 104, 400, 526, 659, 1118
HpaII CCGG 4 cut(s) 26, 614, 849, 977
HphI GGTGA 4 cut(s) 497, 754, 838, 991
Hpy166II GTNNAC 4 cut(s) 289, 549, 735, 814
Hpy188I TCNGA 8 cut(s) 45, 59, 168, 525, 675, 685, 808, 1057
Hpy188III TCNNGA 5 cut(s) 512, 539, 629, 836, 1073
Hpy8I GTNNAC 4 cut(s) 289, 549, 735, 814
HpyAV CCTTC 8 cut(s) 66, 263, 277, 443, 518, 563, 916, 976
HpyCH4III ACNGT 3 cut(s) 443, 818, 918
HpyCH4IV ACGT 1 cut(s) 212
HpyF10VI GCNNNNNNNGC 4 cut(s) 147, 572, 614, 1010
HpyF3I CTNAG 3 cut(s) 237, 699, 962
HpySE526I ACGT 1 cut(s) 212
Hsp92II CATG 4 cut(s) 230, 397, 602, 731
HspAI GCGC 2 cut(s) 4, 160
Kzo9I GATC 6 cut(s) 37, 491, 502, 891, 952, 1189
LguI GCTCTTC 2 cut(s) 850, 879
LmnI GCTCC 1 cut(s) 572
Lsp1109I GCAGC 8 cut(s) 150, 153, 217, 238, 544, 587, 1022, 1150
LweI GCATC 4 cut(s) 137, 318, 650, 1000
MaeI CTAG 5 cut(s) 431, 512, 552, 896, 1187
MaeII ACGT 1 cut(s) 212
MalI GATC 6 cut(s) 39, 493, 504, 893, 954, 1191
MboI GATC 6 cut(s) 37, 491, 502, 891, 952, 1189
MboII GAAGA 4 cut(s) 864, 867, 896, 1179
MfeI CAATTG 1 cut(s) 1005
MflI RGATCY 3 cut(s) 491, 891, 1189
MhlI GDGCHC 2 cut(s) 769, 910
MlsI TGGCCA 1 cut(s) 485
MluCI AATT 7 cut(s) 47, 154, 313, 665, 935, 945, 1005
MluNI TGGCCA 1 cut(s) 485
MlyI GAGTC 2 cut(s) 98, 409
MmeI TCCRAC 2 cut(s) 636, 734
MnlI CCTC 9 cut(s) 20, 25, 51, 232, 266, 493, 546, 874, 1021
Mox20I TGGCCA 1 cut(s) 485
MroXI GAANNNNTTC 1 cut(s) 1171
MscI TGGCCA 1 cut(s) 485
MseI TTAA 5 cut(s) 129, 336, 345, 842, 944
MslI CAYNNNNRTG 2 cut(s) 554, 800
Msp20I TGGCCA 1 cut(s) 485
MspA1I CMGCKG 1 cut(s) 1141
MspCI CTTAAG 1 cut(s) 128
MspI CCGG 4 cut(s) 26, 614, 849, 977
MunI CAATTG 1 cut(s) 1005
Mva1269I GAATGC 1 cut(s) 363
MvnI CGCG 1 cut(s) 630
MwoI GCNNNNNNNGC 4 cut(s) 147, 572, 614, 1010
NdeII GATC 6 cut(s) 37, 491, 502, 891, 952, 1189
NlaIII CATG 4 cut(s) 230, 397, 602, 731
NmeAIII GCCGAG 1 cut(s) 181
NruI TCGCGA 1 cut(s) 630
NsbI TGCGCA 1 cut(s) 161
PciSI GCTCTTC 2 cut(s) 850, 879
PctI GAATGC 1 cut(s) 363
PdmI GAANNNNTTC 1 cut(s) 1171
PfeI GAWTC 3 cut(s) 526, 659, 1118
PkrI GCNGC 9 cut(s) 8, 140, 143, 232, 253, 534, 577, 1012, 1140
PleI GAGTC 2 cut(s) 98, 408
PpsI GAGTC 2 cut(s) 98, 408
PshAI GACNNNNGTC 2 cut(s) 519, 1148
PshBI ATTAAT 1 cut(s) 944
PspPI GGNCC 2 cut(s) 521, 707
PsuI RGATCY 3 cut(s) 491, 891, 1189
PvuII CAGCTG 1 cut(s) 1141
RruI TCGCGA 1 cut(s) 630
RsaI GTAC 6 cut(s) 290, 299, 440, 548, 593, 1065
RsaNI GTAC 6 cut(s) 289, 298, 439, 547, 592, 1064
RseI CAYNNNNRTG 2 cut(s) 554, 800
SapI GCTCTTC 2 cut(s) 850, 879
SaqAI TTAA 5 cut(s) 129, 336, 345, 842, 944
SatI GCNGC 9 cut(s) 7, 139, 142, 231, 252, 533, 576, 1011, 1139
Sau3AI GATC 6 cut(s) 37, 491, 502, 891, 952, 1189
Sau96I GGNCC 2 cut(s) 521, 707
ScaI AGTACT 1 cut(s) 593
SchI GAGTC 2 cut(s) 98, 409
SduI GDGCHC 2 cut(s) 769, 910
SfaNI GCATC 4 cut(s) 137, 318, 650, 1000
SfcI CTRYAG 2 cut(s) 814, 1193
SinI GGWCC 2 cut(s) 521, 707
SmiMI CAYNNNNRTG 2 cut(s) 554, 800
SmlI CTYRAG 5 cut(s) 128, 173, 587, 836, 1073
SmoI CTYRAG 5 cut(s) 128, 173, 587, 836, 1073
Sse9I AATT 7 cut(s) 47, 154, 313, 665, 935, 945, 1005
SsiI CCGC 2 cut(s) 332, 446
SspMI CTAG 5 cut(s) 431, 512, 552, 896, 1187
TaaI ACNGT 3 cut(s) 443, 818, 918
TaiI ACGT 1 cut(s) 215
TasI AATT 7 cut(s) 47, 154, 313, 665, 935, 945, 1005
TatI WGTACW 3 cut(s) 288, 591, 1063
TfiI GAWTC 3 cut(s) 526, 659, 1118
Tru1I TTAA 5 cut(s) 129, 336, 345, 842, 944
Tru9I TTAA 5 cut(s) 129, 336, 345, 842, 944
TscAI CASTG 3 cut(s) 493, 624, 921
TseI GCWGC 9 cut(s) 6, 138, 141, 230, 251, 532, 575, 1010, 1138
TspDTI ATGAA 5 cut(s) 215, 489, 930, 1113, 1131
TspGWI ACGGA 1 cut(s) 945
TspRI CASTG 3 cut(s) 493, 624, 921
Vha464I CTTAAG 1 cut(s) 128
VpaK11BI GGWCC 2 cut(s) 521, 707
VspI ATTAAT 1 cut(s) 944
XapI RAATTY 3 cut(s) 47, 154, 935
XbaI TCTAGA 1 cut(s) 511
XcmI CCANNNNNNNNNTGG 1 cut(s) 493
XmiI GTMKAC 2 cut(s) 734, 813
XmnI GAANNNNTTC 1 cut(s) 1171
XspI CTAG 5 cut(s) 431, 512, 552, 896, 1187
ZrmI AGTACT 1 cut(s) 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.