Rh5DG031900
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0015787
Physical Location & Seq
Forward (+)
2254968 .. 2256369
1402 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG031900.1

Sequence Viewer

Length: 744 bp
ATGACTTTCAGCTTCATTTTCAAAGTTCATAAAGCATGCTTCTCTATATGTGCAGGTAGCAGCAGATGCAGGAATTTGCGCAATCAGATACTCAAGGTGGGAAGTGCAAAGCAATCTGCTCGGCTTTTTACGTTCAATGAGCTTCATGCTGCAACTGAGGGCTTCAAGGCTGCTTGTTTGCTTGGAGAAGGAGGATTTGGGAAGGTGTACAAAGGGTACATAGATAGCATCAATTCTAAACAGGCTGTGGCGGTTAAGAAACTTAACAGGAAAGGAATGCAAGGAAGTAGAGAGTTTTGTGCTGAAGTTATCATGTTGAGTCTGTTTCAACACCCAAACCTTGTCAGTCTAGTAGGGTACTGTGCGGAAGGAGAGCAGAGAGTTTTAGTGTATGAATATATGGCCAGTGGATCTTTGGAGGATCACCTTCTAGTGTCTAATGCAACCTTGTTTGCACAACCACTGTTGAAGGATAGAAGTAAATTTTCATTAATTGCTGATCCGTTGCTTAGGGGAAACTTTCCGGTGAAGGGTCTGCATCAAGCTCTTGCAATTGCAGCAATGTGTCTCAATGAGGAAGTTGATTGCCGACCTGTTATGTCTGATGTTGTACTTGCTCTTGAGCATTTATCCAAGCCAAGAAATGAAGTTGTCATTGATGAGGAATCAAAACACAGCCCTGTGCAGCTGACCAATGTCCAATCTCTAAAACGAAGGAGTTCCAAAGCAGAGCTAGATCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

247

Amino Acids

27.3

Weight (kDa)

8.99

Isoelectric Point (pI)

41.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 59 - 153 9.5e-20 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 60 - 144 8.4e-14 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015787)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61860
fragaria_vesca FvH4_3g02440
malus_domestica MD05G1343000.v1.1 MD10G1318000.v1.1
prunus_persica Prupe.4G023800_v2.0.a1
pyrus_communis pycom05g31250
rosa_chinensis RchiOBHm_Chr5g0003691
rosa_laevigata RLG00000031166
rosa_multiflora Rmu_sc0000500.1_g000001
rosa_rugosa Rorug04G0405900
rosa_samantha Rh5AG032700 Rh5BG031300 Rh5CG035400 Rh5DG031900
rosa_wichuraiana Rw5G003030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 80
Acc36I ACCTGC 1 cut(s) 44
AciI CCGC 2 cut(s) 251, 365
AclWI GGATC 3 cut(s) 418, 429, 494
AcoI YGGCCR 1 cut(s) 402
AcsI RAATTY 2 cut(s) 73, 482
AcuI CTGAAG 1 cut(s) 324
AfaI GTAC 4 cut(s) 209, 218, 359, 612
AfiI CCNNNNNNNGG 1 cut(s) 530
AgsI TTSAA 5 cut(s) 22, 136, 166, 329, 469
AjuI GAANNNNNNNTTGG 2 cut(s) 180, 212
AluBI AGCT 5 cut(s) 12, 142, 545, 688, 733
AluI AGCT 5 cut(s) 12, 142, 545, 688, 733
Alw26I GTCTC 1 cut(s) 572
AlwI GGATC 3 cut(s) 418, 429, 494
AoxI GGCC 1 cut(s) 402
ApeKI GCWGC 5 cut(s) 60, 149, 170, 557, 685
ApoI RAATTY 2 cut(s) 73, 482
AseI ATTAAT 1 cut(s) 491
Asp700I GAANNNNTTC 1 cut(s) 718
AspLEI GCGC 1 cut(s) 81
AsuHPI GGTGA 2 cut(s) 416, 538
BalI TGGCCA 1 cut(s) 404
BbvI GCAGC 5 cut(s) 72, 136, 157, 569, 697
BcgI CGANNNNNNTGC 2 cut(s) 101, 135
BcoDI GTCTC 1 cut(s) 572
BfaI CTAG 3 cut(s) 350, 431, 734
BfmI CTRYAG 1 cut(s) 740
BfuAI ACCTGC 1 cut(s) 44
BglII AGATCT 1 cut(s) 736
BisI GCNGC 5 cut(s) 61, 150, 171, 558, 686
BlsI GCNGC 5 cut(s) 62, 151, 172, 559, 687
BmsI GCATC 3 cut(s) 56, 237, 547
BoxI GACNNNNGTC 1 cut(s) 695
Bpu10I CCTNAGC 1 cut(s) 509
BpuEI CTTGAG 2 cut(s) 77, 641
BsaWI WCCGGW 1 cut(s) 523
Bsc4I CCNNNNNNNGG 1 cut(s) 530
Bse1I ACTGG 1 cut(s) 405
Bse3DI GCAATG 1 cut(s) 567
BseLI CCNNNNNNNGG 1 cut(s) 530
BseMI GCAATG 1 cut(s) 567
BseMII CTCAG 1 cut(s) 147
BseNI ACTGG 1 cut(s) 405
BseXI GCAGC 5 cut(s) 72, 136, 157, 569, 697
BsgI GTGCAG 2 cut(s) 72, 704
BshFI GGCC 1 cut(s) 404
BsiSI CCGG 1 cut(s) 524
BslI CCNNNNNNNGG 1 cut(s) 530
BsmAI GTCTC 1 cut(s) 572
BsmI GAATGC 1 cut(s) 282
BsnI GGCC 1 cut(s) 404
Bsp1407I TGTACA 1 cut(s) 207
Bsp143I GATC 4 cut(s) 410, 421, 499, 736
BspACI CCGC 2 cut(s) 251, 365
BspANI GGCC 1 cut(s) 404
BspCNI CTCAG 1 cut(s) 148
BspMI ACCTGC 1 cut(s) 44
BspPI GGATC 3 cut(s) 418, 429, 494
BsrDI GCAATG 1 cut(s) 567
BsrGI TGTACA 1 cut(s) 207
BsrI ACTGG 1 cut(s) 405
BssMI GATC 4 cut(s) 410, 421, 499, 736
Bst4CI ACNGT 2 cut(s) 362, 465
BstAPI GCANNNNNTGC 1 cut(s) 66
BstAUI TGTACA 1 cut(s) 207
BstC8I GCNNGC 1 cut(s) 37
BstDEI CTNAG 2 cut(s) 156, 509
BstHHI GCGC 1 cut(s) 81
BstKTI GATC 4 cut(s) 413, 424, 502, 739
BstMAI GTCTC 1 cut(s) 572
BstMBI GATC 4 cut(s) 410, 421, 499, 736
BstMWI GCNNNNNNNGC 2 cut(s) 66, 557
BstNSI RCATGY 1 cut(s) 39
BstPAI GACNNNNGTC 1 cut(s) 695
BstSFI CTRYAG 1 cut(s) 740
BstV1I GCAGC 5 cut(s) 72, 136, 157, 569, 697
BstX2I RGATCY 2 cut(s) 410, 736
BstYI RGATCY 2 cut(s) 410, 736
BsuRI GGCC 1 cut(s) 404
BtsIMutI CAGTG 2 cut(s) 412, 461
BveI ACCTGC 1 cut(s) 44
Cac8I GCNNGC 1 cut(s) 37
CfoI GCGC 1 cut(s) 81
Csp6I GTAC 4 cut(s) 208, 217, 358, 611
CviAII CATG 3 cut(s) 36, 146, 313
CviQI GTAC 4 cut(s) 208, 217, 358, 611
DdeI CTNAG 2 cut(s) 156, 509
DpnI GATC 4 cut(s) 412, 423, 501, 738
DpnII GATC 4 cut(s) 410, 421, 499, 736
EaeI YGGCCR 1 cut(s) 402
Eco57I CTGAAG 1 cut(s) 324
FaeI CATG 3 cut(s) 39, 149, 316
FatI CATG 3 cut(s) 35, 145, 312
Fnu4HI GCNGC 5 cut(s) 61, 150, 171, 558, 686
Fsp4HI GCNGC 5 cut(s) 61, 150, 171, 558, 686
FspBI CTAG 3 cut(s) 350, 431, 734
FspI TGCGCA 1 cut(s) 80
GlaI GCGC 1 cut(s) 80
GluI GCNGC 5 cut(s) 61, 150, 171, 558, 686
HaeIII GGCC 1 cut(s) 404
HapII CCGG 1 cut(s) 524
HhaI GCGC 1 cut(s) 81
Hin1II CATG 3 cut(s) 39, 149, 316
Hin6I GCGC 1 cut(s) 79
HinP1I GCGC 1 cut(s) 79
HinfI GANTC 2 cut(s) 319, 665
HpaII CCGG 1 cut(s) 524
HphI GGTGA 2 cut(s) 416, 538
Hpy166II GTNNAC 1 cut(s) 208
Hpy188I TCNGA 2 cut(s) 87, 604
Hpy188III TCNNGA 1 cut(s) 620
Hpy8I GTNNAC 1 cut(s) 208
HpyAV CCTTC 7 cut(s) 182, 196, 362, 437, 463, 523, 708
HpyCH4III ACNGT 2 cut(s) 362, 465
HpyCH4IV ACGT 1 cut(s) 131
HpyF10VI GCNNNNNNNGC 2 cut(s) 66, 557
HpyF3I CTNAG 2 cut(s) 156, 509
HpySE526I ACGT 1 cut(s) 131
Hsp92II CATG 3 cut(s) 39, 149, 316
HspAI GCGC 1 cut(s) 79
Kzo9I GATC 4 cut(s) 410, 421, 499, 736
LpnPI CCDG 8 cut(s) 39, 55, 227, 253, 418, 537, 606, 693
Lsp1109I GCAGC 5 cut(s) 72, 136, 157, 569, 697
LweI GCATC 3 cut(s) 56, 237, 547
MaeI CTAG 3 cut(s) 350, 431, 734
MaeII ACGT 1 cut(s) 131
MalI GATC 4 cut(s) 412, 423, 501, 738
MboI GATC 4 cut(s) 410, 421, 499, 736
MfeI CAATTG 1 cut(s) 552
MflI RGATCY 2 cut(s) 410, 736
MlsI TGGCCA 1 cut(s) 404
MluCI AATT 5 cut(s) 73, 232, 482, 492, 552
MluNI TGGCCA 1 cut(s) 404
MlyI GAGTC 1 cut(s) 328
MnlI CCTC 5 cut(s) 151, 185, 412, 568, 655
Mox20I TGGCCA 1 cut(s) 404
MroXI GAANNNNTTC 1 cut(s) 718
MscI TGGCCA 1 cut(s) 404
MseI TTAA 3 cut(s) 255, 264, 491
Msp20I TGGCCA 1 cut(s) 404
MspA1I CMGCKG 1 cut(s) 688
MspI CCGG 1 cut(s) 524
MunI CAATTG 1 cut(s) 552
Mva1269I GAATGC 1 cut(s) 282
MwoI GCNNNNNNNGC 2 cut(s) 66, 557
NdeII GATC 4 cut(s) 410, 421, 499, 736
NlaIII CATG 3 cut(s) 39, 149, 316
NmeAIII GCCGAG 1 cut(s) 100
NsbI TGCGCA 1 cut(s) 80
NspI RCATGY 1 cut(s) 39
PaeI GCATGC 1 cut(s) 39
PctI GAATGC 1 cut(s) 282
PdmI GAANNNNTTC 1 cut(s) 718
PfeI GAWTC 1 cut(s) 665
PkrI GCNGC 5 cut(s) 62, 151, 172, 559, 687
PleI GAGTC 1 cut(s) 327
PpsI GAGTC 1 cut(s) 327
PshAI GACNNNNGTC 1 cut(s) 695
PshBI ATTAAT 1 cut(s) 491
PsuI RGATCY 2 cut(s) 410, 736
PvuII CAGCTG 1 cut(s) 688
RsaI GTAC 4 cut(s) 209, 218, 359, 612
RsaNI GTAC 4 cut(s) 208, 217, 358, 611
SaqAI TTAA 3 cut(s) 255, 264, 491
SatI GCNGC 5 cut(s) 61, 150, 171, 558, 686
Sau3AI GATC 4 cut(s) 410, 421, 499, 736
SchI GAGTC 1 cut(s) 328
SfaNI GCATC 3 cut(s) 56, 237, 547
SfcI CTRYAG 1 cut(s) 740
SmlI CTYRAG 2 cut(s) 92, 620
SmoI CTYRAG 2 cut(s) 92, 620
SphI GCATGC 1 cut(s) 39
Sse9I AATT 5 cut(s) 73, 232, 482, 492, 552
SsiI CCGC 2 cut(s) 251, 365
SspMI CTAG 3 cut(s) 350, 431, 734
TaaI ACNGT 2 cut(s) 362, 465
TaiI ACGT 1 cut(s) 134
TasI AATT 5 cut(s) 73, 232, 482, 492, 552
TatI WGTACW 2 cut(s) 207, 610
TfiI GAWTC 1 cut(s) 665
Tru1I TTAA 3 cut(s) 255, 264, 491
Tru9I TTAA 3 cut(s) 255, 264, 491
TscAI CASTG 2 cut(s) 412, 468
TseI GCWGC 5 cut(s) 60, 149, 170, 557, 685
TspDTI ATGAA 6 cut(s) 4, 17, 134, 408, 477, 660
TspGWI ACGGA 1 cut(s) 492
TspRI CASTG 2 cut(s) 412, 468
VspI ATTAAT 1 cut(s) 491
XapI RAATTY 2 cut(s) 73, 482
XceI RCATGY 1 cut(s) 39
XcmI CCANNNNNNNNNTGG 1 cut(s) 412
XmnI GAANNNNTTC 1 cut(s) 718
XspI CTAG 3 cut(s) 350, 431, 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.