Rh5BG031300
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0015787
Physical Location & Seq
Forward (+)
2207411 .. 2209125
1715 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG031300.1

Sequence Viewer

Length: 825 bp
ATGCGCTGCTTCTCTTTTTGCAAGCCGGTGGAGGATGATCCTCCGAAATTTGTAGTCAGAAAGACAGGCCCAAGGCCATATTCTAATACTGCCACGACATTGGACTCCTTTTTCGCAACGCTCTCCCTTAAGTCAGGTAGCAGCAGATGCAGGAATTTGCGCAATCAGATACTCAAGGTGGGAAGTGCAAAGCAATCTGCTCGGCTTTTTACGTTCAATGAGCTTCATGCTGCAACTGAGGGCTTCAAGGCTGCTTGTTTGCTTGGAGAAGGAGGATTTGGGAAGGTGTACAAAGGGTACATAGATAGCATCAATTCTAAACAGGCTGTGGCGGTTAAGAAACTTAACAGGAAAGGAATGCAAGGAAGTAGAGAGTTTTGTGCTGAAGTTATCATGTTGAGTCTGTTTCAACACCCAAACCTTGTCAGTCTAGTAGGGTACTGTGCGGAAGGAGAGCAGAGAGTTTTAGTGTATGAATATATGGCCAGTGGATCTCTGGAGGATCACCTTCTAGTGTCAAATGCAACCTTGTTTGCACAACCACTGTTGAAGGATAGAAGTAAATTTTCATTAATTGCTGATCCGTTGCTTAGGGGAAACTTTCCGGTGAAGGGTCTGCATCAAGCTCTTGCAATTGCAGCAATGTGTCTCAATGAGGAAGCTGATTGCCGACCTGTAATGTCTGATGTTGTACTTGCTCTTGAGCATTTATCCAAGCCAAGAAATGAAGTTGTCATTGATGATGAATCAAAACACAGCCCTGTGCAGCTGACCAATGTCCAATCTCTAAAACGAAGGAGTTCCAAAGCAGAGCTAGATCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

274

Amino Acids

30.26

Weight (kDa)

9.06

Isoelectric Point (pI)

36.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 86 - 180 1.2e-19 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 87 - 171 9.7e-14 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015787)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61860
fragaria_vesca FvH4_3g02440
malus_domestica MD05G1343000.v1.1 MD10G1318000.v1.1
prunus_persica Prupe.4G023800_v2.0.a1
pyrus_communis pycom05g31250
rosa_chinensis RchiOBHm_Chr5g0003691
rosa_laevigata RLG00000031166
rosa_multiflora Rmu_sc0000500.1_g000001
rosa_rugosa Rorug04G0405900
rosa_samantha Rh5AG032700 Rh5BG031300 Rh5CG035400 Rh5DG031900
rosa_wichuraiana Rw5G003030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 161
AciI CCGC 2 cut(s) 332, 446
AclWI GGATC 4 cut(s) 32, 499, 510, 575
AcoI YGGCCR 1 cut(s) 483
AcsI RAATTY 3 cut(s) 47, 154, 563
AcuI CTGAAG 1 cut(s) 405
AfaI GTAC 4 cut(s) 290, 299, 440, 693
AfiI CCNNNNNNNGG 1 cut(s) 611
AflII CTTAAG 1 cut(s) 128
AgsI TTSAA 4 cut(s) 217, 247, 410, 550
AjuI GAANNNNNNNTTGG 4 cut(s) 64, 96, 261, 293
AluBI AGCT 5 cut(s) 223, 626, 662, 769, 814
AluI AGCT 5 cut(s) 223, 626, 662, 769, 814
Alw26I GTCTC 1 cut(s) 653
AlwI GGATC 4 cut(s) 32, 499, 510, 575
AoxI GGCC 3 cut(s) 67, 74, 483
ApeKI GCWGC 6 cut(s) 6, 141, 230, 251, 638, 766
ApoI RAATTY 3 cut(s) 47, 154, 563
ArsI GACNNNNNNTTYG 2 cut(s) 95, 127
AseI ATTAAT 1 cut(s) 572
Asp700I GAANNNNTTC 1 cut(s) 799
AspLEI GCGC 2 cut(s) 6, 162
AspS9I GGNCC 1 cut(s) 68
AsuHPI GGTGA 2 cut(s) 497, 619
BalI TGGCCA 1 cut(s) 485
BbvI GCAGC 5 cut(s) 153, 217, 238, 650, 778
BcgI CGANNNNNNTGC 2 cut(s) 182, 216
BcoDI GTCTC 1 cut(s) 653
BfaI CTAG 3 cut(s) 431, 512, 815
BfmI CTRYAG 1 cut(s) 821
BfrI CTTAAG 1 cut(s) 128
BglII AGATCT 1 cut(s) 817
BisI GCNGC 6 cut(s) 7, 142, 231, 252, 639, 767
BlsI GCNGC 6 cut(s) 8, 143, 232, 253, 640, 768
BmgT120I GGNCC 1 cut(s) 68
BmsI GCATC 3 cut(s) 137, 318, 628
BoxI GACNNNNGTC 1 cut(s) 776
BpmI CTGGAG 1 cut(s) 518
Bpu10I CCTNAGC 1 cut(s) 590
BpuEI CTTGAG 2 cut(s) 158, 722
BsaJI CCNNGG 1 cut(s) 71
BsaWI WCCGGW 1 cut(s) 604
Bsc4I CCNNNNNNNGG 1 cut(s) 611
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 1 cut(s) 486
Bse3DI GCAATG 1 cut(s) 648
BseDI CCNNGG 1 cut(s) 71
BseGI GGATG 1 cut(s) 40
BseLI CCNNNNNNNGG 1 cut(s) 611
BseMI GCAATG 1 cut(s) 648
BseMII CTCAG 1 cut(s) 228
BseNI ACTGG 1 cut(s) 486
BseXI GCAGC 5 cut(s) 153, 217, 238, 650, 778
BsgI GTGCAG 1 cut(s) 785
BshFI GGCC 3 cut(s) 69, 76, 485
BsiSI CCGG 2 cut(s) 26, 605
BslI CCNNNNNNNGG 1 cut(s) 611
BsmAI GTCTC 1 cut(s) 653
BsmI GAATGC 1 cut(s) 363
BsnI GGCC 3 cut(s) 69, 76, 485
Bsp1407I TGTACA 1 cut(s) 288
Bsp143I GATC 5 cut(s) 37, 491, 502, 580, 817
BspACI CCGC 2 cut(s) 332, 446
BspANI GGCC 3 cut(s) 69, 76, 485
BspCNI CTCAG 1 cut(s) 229
BspPI GGATC 4 cut(s) 32, 499, 510, 575
BspTI CTTAAG 1 cut(s) 128
BsrDI GCAATG 1 cut(s) 648
BsrFI RCCGGY 1 cut(s) 25
BsrGI TGTACA 1 cut(s) 288
BsrI ACTGG 1 cut(s) 486
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 1 cut(s) 71
BssMI GATC 5 cut(s) 37, 491, 502, 580, 817
BssT1I CCWWGG 1 cut(s) 71
Bst4CI ACNGT 2 cut(s) 443, 546
BstAFI CTTAAG 1 cut(s) 128
BstAPI GCANNNNNTGC 1 cut(s) 147
BstAUI TGTACA 1 cut(s) 288
BstC8I GCNNGC 1 cut(s) 23
BstDEI CTNAG 2 cut(s) 237, 590
BstF5I GGATG 1 cut(s) 40
BstHHI GCGC 2 cut(s) 6, 162
BstKTI GATC 5 cut(s) 40, 494, 505, 583, 820
BstMAI GTCTC 1 cut(s) 653
BstMBI GATC 5 cut(s) 37, 491, 502, 580, 817
BstMWI GCNNNNNNNGC 2 cut(s) 147, 638
BstPAI GACNNNNGTC 1 cut(s) 776
BstSFI CTRYAG 1 cut(s) 821
BstV1I GCAGC 5 cut(s) 153, 217, 238, 650, 778
BstX2I RGATCY 2 cut(s) 491, 817
BstXI CCANNNNNNTGG 1 cut(s) 100
BstYI RGATCY 2 cut(s) 491, 817
BsuRI GGCC 3 cut(s) 69, 76, 485
BtsCI GGATG 1 cut(s) 40
BtsIMutI CAGTG 2 cut(s) 493, 542
Cac8I GCNNGC 1 cut(s) 23
CfoI GCGC 2 cut(s) 6, 162
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 1 cut(s) 68
Csp6I GTAC 4 cut(s) 289, 298, 439, 692
CviAII CATG 2 cut(s) 227, 394
CviQI GTAC 4 cut(s) 289, 298, 439, 692
DdeI CTNAG 2 cut(s) 237, 590
DpnI GATC 5 cut(s) 39, 493, 504, 582, 819
DpnII GATC 5 cut(s) 37, 491, 502, 580, 817
EaeI YGGCCR 1 cut(s) 483
Eco130I CCWWGG 1 cut(s) 71
Eco57I CTGAAG 1 cut(s) 405
EcoT14I CCWWGG 1 cut(s) 71
ErhI CCWWGG 1 cut(s) 71
FaeI CATG 2 cut(s) 230, 397
FaiI YATR 8 cut(s) 79, 228, 302, 395, 474, 480, 482, 823
FatI CATG 2 cut(s) 226, 393
Fnu4HI GCNGC 6 cut(s) 7, 142, 231, 252, 639, 767
FokI GGATG 1 cut(s) 47
Fsp4HI GCNGC 6 cut(s) 7, 142, 231, 252, 639, 767
FspBI CTAG 3 cut(s) 431, 512, 815
FspI TGCGCA 1 cut(s) 161
GlaI GCGC 2 cut(s) 5, 161
GluI GCNGC 6 cut(s) 7, 142, 231, 252, 639, 767
GsuI CTGGAG 1 cut(s) 518
HaeIII GGCC 3 cut(s) 69, 76, 485
HapII CCGG 2 cut(s) 26, 605
HhaI GCGC 2 cut(s) 6, 162
Hin1II CATG 2 cut(s) 230, 397
Hin6I GCGC 2 cut(s) 4, 160
HinP1I GCGC 2 cut(s) 4, 160
HinfI GANTC 3 cut(s) 104, 400, 746
HpaII CCGG 2 cut(s) 26, 605
HphI GGTGA 2 cut(s) 497, 619
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 4 cut(s) 45, 59, 168, 685
Hpy188III TCNNGA 2 cut(s) 497, 701
Hpy8I GTNNAC 1 cut(s) 289
HpyAV CCTTC 7 cut(s) 263, 277, 443, 518, 544, 604, 789
HpyCH4III ACNGT 2 cut(s) 443, 546
HpyCH4IV ACGT 1 cut(s) 212
HpyF10VI GCNNNNNNNGC 2 cut(s) 147, 638
HpyF3I CTNAG 2 cut(s) 237, 590
HpySE526I ACGT 1 cut(s) 212
Hsp92II CATG 2 cut(s) 230, 397
HspAI GCGC 2 cut(s) 4, 160
Kzo9I GATC 5 cut(s) 37, 491, 502, 580, 817
Lsp1109I GCAGC 5 cut(s) 153, 217, 238, 650, 778
LweI GCATC 3 cut(s) 137, 318, 628
MaeI CTAG 3 cut(s) 431, 512, 815
MaeII ACGT 1 cut(s) 212
MalI GATC 5 cut(s) 39, 493, 504, 582, 819
MboI GATC 5 cut(s) 37, 491, 502, 580, 817
MfeI CAATTG 1 cut(s) 633
MflI RGATCY 2 cut(s) 491, 817
MlsI TGGCCA 1 cut(s) 485
MluCI AATT 6 cut(s) 47, 154, 313, 563, 573, 633
MluNI TGGCCA 1 cut(s) 485
MlyI GAGTC 2 cut(s) 98, 409
MnlI CCTC 6 cut(s) 25, 51, 232, 266, 493, 649
Mox20I TGGCCA 1 cut(s) 485
MroXI GAANNNNTTC 1 cut(s) 799
MscI TGGCCA 1 cut(s) 485
MseI TTAA 4 cut(s) 129, 336, 345, 572
Msp20I TGGCCA 1 cut(s) 485
MspA1I CMGCKG 1 cut(s) 769
MspCI CTTAAG 1 cut(s) 128
MspI CCGG 2 cut(s) 26, 605
MunI CAATTG 1 cut(s) 633
Mva1269I GAATGC 1 cut(s) 363
MwoI GCNNNNNNNGC 2 cut(s) 147, 638
NdeII GATC 5 cut(s) 37, 491, 502, 580, 817
NlaIII CATG 2 cut(s) 230, 397
NmeAIII GCCGAG 1 cut(s) 181
NsbI TGCGCA 1 cut(s) 161
PctI GAATGC 1 cut(s) 363
PdmI GAANNNNTTC 1 cut(s) 799
PfeI GAWTC 1 cut(s) 746
PkrI GCNGC 6 cut(s) 8, 143, 232, 253, 640, 768
PleI GAGTC 2 cut(s) 98, 408
PpsI GAGTC 2 cut(s) 98, 408
PshAI GACNNNNGTC 1 cut(s) 776
PshBI ATTAAT 1 cut(s) 572
PspPI GGNCC 1 cut(s) 68
PsuI RGATCY 2 cut(s) 491, 817
PvuII CAGCTG 1 cut(s) 769
RsaI GTAC 4 cut(s) 290, 299, 440, 693
RsaNI GTAC 4 cut(s) 289, 298, 439, 692
SaqAI TTAA 4 cut(s) 129, 336, 345, 572
SatI GCNGC 6 cut(s) 7, 142, 231, 252, 639, 767
Sau3AI GATC 5 cut(s) 37, 491, 502, 580, 817
Sau96I GGNCC 1 cut(s) 68
SchI GAGTC 2 cut(s) 98, 409
SfaNI GCATC 3 cut(s) 137, 318, 628
SfcI CTRYAG 1 cut(s) 821
SmlI CTYRAG 3 cut(s) 128, 173, 701
SmoI CTYRAG 3 cut(s) 128, 173, 701
Sse9I AATT 6 cut(s) 47, 154, 313, 563, 573, 633
SsiI CCGC 2 cut(s) 332, 446
SspMI CTAG 3 cut(s) 431, 512, 815
StyI CCWWGG 1 cut(s) 71
TaaI ACNGT 2 cut(s) 443, 546
TaiI ACGT 1 cut(s) 215
TasI AATT 6 cut(s) 47, 154, 313, 563, 573, 633
TatI WGTACW 2 cut(s) 288, 691
TfiI GAWTC 1 cut(s) 746
Tru1I TTAA 4 cut(s) 129, 336, 345, 572
Tru9I TTAA 4 cut(s) 129, 336, 345, 572
TscAI CASTG 2 cut(s) 493, 549
TseI GCWGC 6 cut(s) 6, 141, 230, 251, 638, 766
TspDTI ATGAA 5 cut(s) 215, 489, 558, 741, 759
TspGWI ACGGA 1 cut(s) 573
TspRI CASTG 2 cut(s) 493, 549
Vha464I CTTAAG 1 cut(s) 128
VspI ATTAAT 1 cut(s) 572
XapI RAATTY 3 cut(s) 47, 154, 563
XcmI CCANNNNNNNNNTGG 1 cut(s) 493
XmnI GAANNNNTTC 1 cut(s) 799
XspI CTAG 3 cut(s) 431, 512, 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.