Rw5G003030
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0015787
Physical Location & Seq
Forward (+)
2827090 .. 2828487
1398 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G003030.1

Sequence Viewer

Length: 1071 bp
ATGACTTTCAGCTTTATTTTCAAAGTTCATAAAGCATGCTTCTCTATATGTGCAGGTAGCAGCAGATGCAGGAATTTGCGCAATCAGATACTCAAGGTGGGAAGTGCAAAGCAATCTGCTCGGCTTTTTACGTTCAATGAGCTTCATGCTGCAACTGAGGGCTTCAAGGCTGCTTGTTTGCTTGGAGAAGGAGGATTTGGGAAGGTGTACAAAGGGTACATAGATAGCATCAATTCTAAACAGGCTGTGGCGGTTAAGAAACTTAACCGGAAAGGAATGCAAGGAAGTAGAGAGTTTTGTGCTGAAGTTATCATGTTGAGTCTGTTTCAACACCCAAACCTTGTCAGTCTAGTAGGGTACTGTGCGGAAGGAGAGCAGAGAGTTTTAGTGTATGAATATATGGCCAGTGGATCTTTGGAGGATCACCTTCTAGACATAGGTCCGAATCAGCAGCCTCTTGATTGGTACACTAGGATGAGAATAGCAGAAGGAGCAGCTAAAGGACTTGAGTACTTGCATGATGTTGCCAAACCGGCAGTGATTTATCGCGATTTCAAAGCATCAAACATATTGTTGGATTCCCAATTCACTCCGAAGCTGTCTGATTTTGGACTTGCTAAGTTAGGTCCAACAGGAGACAAGTCCCATGTGTCTACAAGGGTGATGGGAACTTATGGCTATTGTGCCCCAGAGTATGCTTGCACAGGAAAGCTCACAAAAATGTCTGATGTCTACAGTTTCGGGGTTGTCTTTCTTGAGTTAATCACCGGAAGAAGAGCTGTTGATGAGACAAAACCAGAGGAAGAGCAGATCCTAGTCAGTTGGGCACAACCACTGTTGAAGGATAGAAGTAAATTTTCATTAATTGCTGATCCGTTGCTTAGGGGAAACTTTCCGGTGAAGGGTCTGCATCAAGCTCTTGCAATTGCAGCAATGTGTCTCAATGAGGAACATTTATCCAAGCCAAGAAATGAAGTTGTCATTGATGATGAATCAAAACACAGCCCTGTGCAGCTGACCAATGTCCAATCTCTAAAACGAAGGAGTTCCAAAGCAGAGCTAGATCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

356

Amino Acids

39.5

Weight (kDa)

8.82

Isoelectric Point (pI)

37.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 59 - 268 5.6e-45 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 60 - 268 7.7e-42 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015787)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61860
fragaria_vesca FvH4_3g02440
malus_domestica MD05G1343000.v1.1 MD10G1318000.v1.1
prunus_persica Prupe.4G023800_v2.0.a1
pyrus_communis pycom05g31250
rosa_chinensis RchiOBHm_Chr5g0003691
rosa_laevigata RLG00000031166
rosa_multiflora Rmu_sc0000500.1_g000001
rosa_rugosa Rorug04G0405900
rosa_samantha Rh5AG032700 Rh5BG031300 Rh5CG035400 Rh5DG031900
rosa_wichuraiana Rw5G003030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 80
Acc36I ACCTGC 1 cut(s) 44
AccI GTMKAC 2 cut(s) 653, 732
AccII CGCG 1 cut(s) 549
AciI CCGC 2 cut(s) 251, 365
AclWI GGATC 4 cut(s) 418, 429, 805, 866
AcoI YGGCCR 1 cut(s) 402
AcsI RAATTY 2 cut(s) 73, 854
AcuI CTGAAG 1 cut(s) 324
AfaI GTAC 5 cut(s) 209, 218, 359, 467, 512
AfiI CCNNNNNNNGG 1 cut(s) 902
AgsI TTSAA 6 cut(s) 22, 136, 166, 329, 556, 841
AjuI GAANNNNNNNTTGG 2 cut(s) 180, 212
AluBI AGCT 9 cut(s) 12, 142, 497, 598, 712, 779, 917, 1015, 1060
AluI AGCT 9 cut(s) 12, 142, 497, 598, 712, 779, 917, 1015, 1060
Alw26I GTCTC 3 cut(s) 630, 782, 944
AlwI GGATC 4 cut(s) 418, 429, 805, 866
AoxI GGCC 1 cut(s) 402
ApeKI GCWGC 7 cut(s) 60, 149, 170, 451, 494, 929, 1012
ApoI RAATTY 2 cut(s) 73, 854
AseI ATTAAT 1 cut(s) 863
Asp700I GAANNNNTTC 1 cut(s) 1045
AspLEI GCGC 1 cut(s) 81
AspS9I GGNCC 2 cut(s) 440, 626
AsuHPI GGTGA 4 cut(s) 416, 673, 757, 910
AvaII GGWCC 2 cut(s) 440, 626
BaeGI GKGCMC 2 cut(s) 688, 829
BalI TGGCCA 1 cut(s) 404
BbvI GCAGC 7 cut(s) 72, 136, 157, 463, 506, 941, 1024
BccI CCATC 1 cut(s) 658
BcgI CGANNNNNNTGC 2 cut(s) 101, 135
BcoDI GTCTC 3 cut(s) 630, 782, 944
BfaI CTAG 5 cut(s) 350, 431, 471, 815, 1061
BfmI CTRYAG 2 cut(s) 733, 1067
BfuAI ACCTGC 1 cut(s) 44
BglI GCCNNNNNGGC 1 cut(s) 533
BglII AGATCT 1 cut(s) 1063
BisI GCNGC 7 cut(s) 61, 150, 171, 452, 495, 930, 1013
BlsI GCNGC 7 cut(s) 62, 151, 172, 453, 496, 931, 1014
BmcAI AGTACT 1 cut(s) 512
Bme18I GGWCC 2 cut(s) 440, 626
BmgT120I GGNCC 2 cut(s) 440, 626
BmsI GCATC 4 cut(s) 56, 237, 569, 919
BoxI GACNNNNGTC 2 cut(s) 438, 1022
Bpu10I CCTNAGC 1 cut(s) 881
BpuEI CTTGAG 3 cut(s) 77, 527, 776
BsaWI WCCGGW 3 cut(s) 267, 767, 895
Bsc4I CCNNNNNNNGG 1 cut(s) 902
Bse118I RCCGGY 1 cut(s) 532
Bse1I ACTGG 1 cut(s) 405
Bse3DI GCAATG 1 cut(s) 939
BseGI GGATG 1 cut(s) 480
BseLI CCNNNNNNNGG 1 cut(s) 902
BseMI GCAATG 1 cut(s) 939
BseMII CTCAG 1 cut(s) 147
BseNI ACTGG 1 cut(s) 405
BseSI GKGCMC 2 cut(s) 688, 829
BseXI GCAGC 7 cut(s) 72, 136, 157, 463, 506, 941, 1024
BsgI GTGCAG 2 cut(s) 72, 1031
Bsh1236I CGCG 1 cut(s) 549
BshFI GGCC 1 cut(s) 404
BsiSI CCGG 4 cut(s) 268, 533, 768, 896
BslFI GGGAC 1 cut(s) 628
BslI CCNNNNNNNGG 1 cut(s) 902
BsmAI GTCTC 3 cut(s) 630, 782, 944
BsmFI GGGAC 1 cut(s) 628
BsmI GAATGC 1 cut(s) 282
BsnI GGCC 1 cut(s) 404
Bsp1286I GDGCHC 2 cut(s) 688, 829
Bsp1407I TGTACA 1 cut(s) 207
Bsp143I GATC 5 cut(s) 410, 421, 810, 871, 1063
Bsp68I TCGCGA 1 cut(s) 549
BspACI CCGC 2 cut(s) 251, 365
BspANI GGCC 1 cut(s) 404
BspCNI CTCAG 1 cut(s) 148
BspFNI CGCG 1 cut(s) 549
BspMI ACCTGC 1 cut(s) 44
BspPI GGATC 4 cut(s) 418, 429, 805, 866
BspQI GCTCTTC 2 cut(s) 769, 798
BsrDI GCAATG 1 cut(s) 939
BsrFI RCCGGY 1 cut(s) 532
BsrGI TGTACA 1 cut(s) 207
BsrI ACTGG 1 cut(s) 405
BssAI RCCGGY 1 cut(s) 532
BssMI GATC 5 cut(s) 410, 421, 810, 871, 1063
Bst4CI ACNGT 3 cut(s) 362, 737, 837
Bst6I CTCTTC 2 cut(s) 769, 798
BstAPI GCANNNNNTGC 1 cut(s) 66
BstAUI TGTACA 1 cut(s) 207
BstC8I GCNNGC 2 cut(s) 37, 700
BstDEI CTNAG 3 cut(s) 156, 618, 881
BstF5I GGATG 1 cut(s) 480
BstFNI CGCG 1 cut(s) 549
BstHHI GCGC 1 cut(s) 81
BstKTI GATC 5 cut(s) 413, 424, 813, 874, 1066
BstMAI GTCTC 3 cut(s) 630, 782, 944
BstMBI GATC 5 cut(s) 410, 421, 810, 871, 1063
BstMWI GCNNNNNNNGC 4 cut(s) 66, 491, 533, 929
BstNSI RCATGY 1 cut(s) 39
BstPAI GACNNNNGTC 2 cut(s) 438, 1022
BstSFI CTRYAG 2 cut(s) 733, 1067
BstSLI GKGCMC 2 cut(s) 688, 829
BstUI CGCG 1 cut(s) 549
BstV1I GCAGC 7 cut(s) 72, 136, 157, 463, 506, 941, 1024
BstX2I RGATCY 3 cut(s) 410, 810, 1063
BstYI RGATCY 3 cut(s) 410, 810, 1063
BsuRI GGCC 1 cut(s) 404
BtsCI GGATG 1 cut(s) 480
BtsI GCAGTG 1 cut(s) 543
BtsIMutI CAGTG 3 cut(s) 412, 543, 833
BtuMI TCGCGA 1 cut(s) 549
BveI ACCTGC 1 cut(s) 44
Cac8I GCNNGC 2 cut(s) 37, 700
CfoI GCGC 1 cut(s) 81
Cfr10I RCCGGY 1 cut(s) 532
Cfr13I GGNCC 2 cut(s) 440, 626
Csp6I GTAC 5 cut(s) 208, 217, 358, 466, 511
CviAII CATG 5 cut(s) 36, 146, 313, 518, 647
CviQI GTAC 5 cut(s) 208, 217, 358, 466, 511
DdeI CTNAG 3 cut(s) 156, 618, 881
DpnI GATC 5 cut(s) 412, 423, 812, 873, 1065
DpnII GATC 5 cut(s) 410, 421, 810, 871, 1063
EaeI YGGCCR 1 cut(s) 402
Eam1104I CTCTTC 2 cut(s) 769, 798
EarI CTCTTC 2 cut(s) 769, 798
Eco47I GGWCC 2 cut(s) 440, 626
Eco57I CTGAAG 1 cut(s) 324
FaeI CATG 5 cut(s) 39, 149, 316, 521, 650
FaqI GGGAC 1 cut(s) 628
FatI CATG 5 cut(s) 35, 145, 312, 517, 646
FblI GTMKAC 2 cut(s) 653, 732
Fnu4HI GCNGC 7 cut(s) 61, 150, 171, 452, 495, 930, 1013
FokI GGATG 1 cut(s) 487
Fsp4HI GCNGC 7 cut(s) 61, 150, 171, 452, 495, 930, 1013
FspBI CTAG 5 cut(s) 350, 431, 471, 815, 1061
FspI TGCGCA 1 cut(s) 80
GlaI GCGC 1 cut(s) 80
GluI GCNGC 7 cut(s) 61, 150, 171, 452, 495, 930, 1013
HaeIII GGCC 1 cut(s) 404
HapII CCGG 4 cut(s) 268, 533, 768, 896
HhaI GCGC 1 cut(s) 81
Hin1II CATG 5 cut(s) 39, 149, 316, 521, 650
Hin6I GCGC 1 cut(s) 79
HinP1I GCGC 1 cut(s) 79
HinfI GANTC 4 cut(s) 319, 445, 578, 992
HpaII CCGG 4 cut(s) 268, 533, 768, 896
HphI GGTGA 4 cut(s) 416, 673, 757, 910
Hpy166II GTNNAC 4 cut(s) 208, 468, 654, 733
Hpy188I TCNGA 5 cut(s) 87, 444, 594, 604, 727
Hpy188III TCNNGA 4 cut(s) 431, 458, 548, 755
Hpy8I GTNNAC 4 cut(s) 208, 468, 654, 733
HpyAV CCTTC 8 cut(s) 182, 196, 362, 437, 482, 835, 895, 1035
HpyCH4III ACNGT 3 cut(s) 362, 737, 837
HpyCH4IV ACGT 1 cut(s) 131
HpyF10VI GCNNNNNNNGC 4 cut(s) 66, 491, 533, 929
HpyF3I CTNAG 3 cut(s) 156, 618, 881
HpySE526I ACGT 1 cut(s) 131
Hsp92II CATG 5 cut(s) 39, 149, 316, 521, 650
HspAI GCGC 1 cut(s) 79
Kzo9I GATC 5 cut(s) 410, 421, 810, 871, 1063
LguI GCTCTTC 2 cut(s) 769, 798
LmnI GCTCC 1 cut(s) 491
Lsp1109I GCAGC 7 cut(s) 72, 136, 157, 463, 506, 941, 1024
LweI GCATC 4 cut(s) 56, 237, 569, 919
MaeI CTAG 5 cut(s) 350, 431, 471, 815, 1061
MaeII ACGT 1 cut(s) 131
MalI GATC 5 cut(s) 412, 423, 812, 873, 1065
MboI GATC 5 cut(s) 410, 421, 810, 871, 1063
MboII GAAGA 3 cut(s) 783, 786, 815
MfeI CAATTG 1 cut(s) 924
MflI RGATCY 3 cut(s) 410, 810, 1063
MhlI GDGCHC 2 cut(s) 688, 829
MlsI TGGCCA 1 cut(s) 404
MluCI AATT 6 cut(s) 73, 232, 584, 854, 864, 924
MluNI TGGCCA 1 cut(s) 404
MlyI GAGTC 1 cut(s) 328
MmeI TCCRAC 2 cut(s) 555, 653
MnlI CCTC 6 cut(s) 151, 185, 412, 465, 793, 940
Mox20I TGGCCA 1 cut(s) 404
MroXI GAANNNNTTC 1 cut(s) 1045
MscI TGGCCA 1 cut(s) 404
MseI TTAA 4 cut(s) 255, 264, 761, 863
MslI CAYNNNNRTG 2 cut(s) 473, 719
Msp20I TGGCCA 1 cut(s) 404
MspA1I CMGCKG 1 cut(s) 1015
MspI CCGG 4 cut(s) 268, 533, 768, 896
MunI CAATTG 1 cut(s) 924
Mva1269I GAATGC 1 cut(s) 282
MvnI CGCG 1 cut(s) 549
MwoI GCNNNNNNNGC 4 cut(s) 66, 491, 533, 929
NdeII GATC 5 cut(s) 410, 421, 810, 871, 1063
NlaIII CATG 5 cut(s) 39, 149, 316, 521, 650
NmeAIII GCCGAG 1 cut(s) 100
NruI TCGCGA 1 cut(s) 549
NsbI TGCGCA 1 cut(s) 80
NspI RCATGY 1 cut(s) 39
PaeI GCATGC 1 cut(s) 39
PciSI GCTCTTC 2 cut(s) 769, 798
PctI GAATGC 1 cut(s) 282
PdmI GAANNNNTTC 1 cut(s) 1045
PfeI GAWTC 3 cut(s) 445, 578, 992
PkrI GCNGC 7 cut(s) 62, 151, 172, 453, 496, 931, 1014
PleI GAGTC 1 cut(s) 327
PpsI GAGTC 1 cut(s) 327
PshAI GACNNNNGTC 2 cut(s) 438, 1022
PshBI ATTAAT 1 cut(s) 863
PspPI GGNCC 2 cut(s) 440, 626
PsuI RGATCY 3 cut(s) 410, 810, 1063
PvuII CAGCTG 1 cut(s) 1015
RruI TCGCGA 1 cut(s) 549
RsaI GTAC 5 cut(s) 209, 218, 359, 467, 512
RsaNI GTAC 5 cut(s) 208, 217, 358, 466, 511
RseI CAYNNNNRTG 2 cut(s) 473, 719
SapI GCTCTTC 2 cut(s) 769, 798
SaqAI TTAA 4 cut(s) 255, 264, 761, 863
SatI GCNGC 7 cut(s) 61, 150, 171, 452, 495, 930, 1013
Sau3AI GATC 5 cut(s) 410, 421, 810, 871, 1063
Sau96I GGNCC 2 cut(s) 440, 626
ScaI AGTACT 1 cut(s) 512
SchI GAGTC 1 cut(s) 328
SduI GDGCHC 2 cut(s) 688, 829
SfaNI GCATC 4 cut(s) 56, 237, 569, 919
SfcI CTRYAG 2 cut(s) 733, 1067
SinI GGWCC 2 cut(s) 440, 626
SmiMI CAYNNNNRTG 2 cut(s) 473, 719
SmlI CTYRAG 3 cut(s) 92, 506, 755
SmoI CTYRAG 3 cut(s) 92, 506, 755
SphI GCATGC 1 cut(s) 39
Sse9I AATT 6 cut(s) 73, 232, 584, 854, 864, 924
SsiI CCGC 2 cut(s) 251, 365
SspMI CTAG 5 cut(s) 350, 431, 471, 815, 1061
TaaI ACNGT 3 cut(s) 362, 737, 837
TaiI ACGT 1 cut(s) 134
TasI AATT 6 cut(s) 73, 232, 584, 854, 864, 924
TatI WGTACW 2 cut(s) 207, 510
TfiI GAWTC 3 cut(s) 445, 578, 992
Tru1I TTAA 4 cut(s) 255, 264, 761, 863
Tru9I TTAA 4 cut(s) 255, 264, 761, 863
TscAI CASTG 3 cut(s) 412, 543, 840
TseI GCWGC 7 cut(s) 60, 149, 170, 451, 494, 929, 1012
TspDTI ATGAA 6 cut(s) 17, 134, 408, 849, 987, 1005
TspGWI ACGGA 1 cut(s) 864
TspRI CASTG 3 cut(s) 412, 543, 840
VpaK11BI GGWCC 2 cut(s) 440, 626
VspI ATTAAT 1 cut(s) 863
XapI RAATTY 2 cut(s) 73, 854
XbaI TCTAGA 1 cut(s) 430
XceI RCATGY 1 cut(s) 39
XcmI CCANNNNNNNNNTGG 1 cut(s) 412
XmiI GTMKAC 2 cut(s) 653, 732
XmnI GAANNNNTTC 1 cut(s) 1045
XspI CTAG 5 cut(s) 350, 431, 471, 815, 1061
ZrmI AGTACT 1 cut(s) 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.