Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003730

Physical Location & Seq

Genomic Coordinates Forward (+)

46678533 .. 46682086

Gene Structure 3554 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1348300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1623 bp

ATGAGGAAGCAGCAAAGCTCAAGCTCCTCTCCAATGGCGGTCACTAAGTTTCTCTCACTCTCAACCCTCCTCTTCTTCTTCGTCTTTGCATCCAAAGCCACATCTTCTCCACCGACATTCAAAACCACCAAGCTGCAAAATCAGGACCAAGAAAAAGAGCCGTTCGTCGGAGTGAACATTGGCACCGACGTTTCGAATCTTCTGTCTCCGTCGGACCTCGTTTCGTTTCTGCAACTCCAGCAGATCAACCACGTCCGCCTCTACGATGCCGATCCGGACATCCTCAAAGCCCTGTCCAAAACCAAGATCCGGGTCGTCATCAGCGTCCCCAACAACCAGCTCCTCGCCATCGGCTCTTCCAACACCACCGCCGCTTCCTGGGTCGGCCGAAACGTCGTCGCTTACTACCCCCAAACCCTCATCACTGGCATTGCGGTCGGCGACGAGGTGTTAACCACCGTGCCTTCTTCAGCTCCTCTGCTTCTTCCGGCAATTCAGTCGCTTTACAGTGCTTTAGTGGCTGCAAATTTACACACCCATGTGAAAATTTCGACCCCTCACGCCGCTTCGATAATTCTCGATCCGTTTCCGCCGTCACAGGCGTTCTTCAACCAAAGCTTAACCCAAATTGTTCTCCCATTGCTTCAGTTCTTGTCGAAAACCGGGTCGCCGCTGATGATGAATCTCTATCCTTACTATGTGTTTATGCAGAACAAAGGTGTGGTGCCTCTTGACAATGCGCTTTTCAAGCCTCTTACGCCGTCGAAAGAGATGGTGGATCCCAATACTTTGCTACATTATACTAATGTGCTGGATGCCATGATTGATGCTGCTTATTTTTCCATGAAAAACCTTAATATTACTGATGTGACGGTTCTTGTTTCGGAGAGCGGATGGCCTTCGAAGGGGGATTCTAAAGAGCCTTATGCTACTATCGACAATGCAGATACATACAATTCAAATCTTATCAAGCATGTTTTGGATCGTAGTGGAACCCCTTTGCACCCTGAGATTACTTCAGATGTGTACATTTATGAATTGTTCAATGAGGATTTGAGGTCTCCGCCAGTGTCCGAGGCCAATTGGGGCCTGTTTTATGGGAATTCGAGTGCGGTGTATTTGCTTCACGTATCAGGGAGTGGGTCTTTTTTGGCTAATGACACAACAAACCAGACATACTGCATTGCCATGGATGGGATGGATGGAAAGAGTTTGCAGGCTGCATTGGATTGGGCATGTGGACCTGGGCGGGCAAATTGCTCGGAGATTCAGCCGGGGGAGGATTGTTACTCTCCGAATAATGTGAAAAGCCATGCTTCATATGCATTTGATAGCTATTACCAGAAGCAAGGGAGGGCTGCCGGGTCTTGTGACTTCAATGGCGTAGCCACCATTACCACCACTGACCCAAGTCACGGGAGCTGTATATTTCCCGGAAGTAAGAAGGTGAGCAACAAGACGAGGCAGACGGTGAACTCGACACATTCCAGTAGCGGAGTAGACAGATTAAGATTTGTTGCCTTCGGCAGCAGCAGAATAAGTGCCATCAGCGAGAAGTTATCCGTATTTTTTGTTGTAATCTTCTACAGTTTGTTATTCTTTCCTTTTGTGACTCCATGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001871 GO:0003674 GO:0003824 GO:0004553 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0016787 GO:0016798 GO:0030246 GO:0030247 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00500 map00500

Pfam Domains

Protein Families

Glyco_hydro_17 X8

Protein Analysis

541

Amino Acids

58.85

Weight (kDa)

6.26

Isoelectric Point (pI)

43.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_17	PF00332	56 - 375	7.3e-72	Glycosyl hydrolases family 17
X8	PF07983	393 - 464	2.9e-22	X8 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003730)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11800
fragaria_vesca	FvH4_3g01860 FvH4_3g01880 FvH4_3g01880 FvH4_3g01880
malus_domestica	MD05G1348300.v1.1 MD10G1323100.v1.1 MD10G1323300.v1.1
prunus_persica	Prupe.4G019000_v2.0.a1 Prupe.4G019000_v2.0.a1 Prupe.4G019100_v2.0.a1
pyrus_communis	pycom05g31580 pycom10g27350 pycom10g27360
rosa_chinensis	RchiOBHm_Chr5g0002731 RchiOBHm_Chr5g0002751
rosa_laevigata	RLG00000031055
rosa_multiflora	Rmu_sc0013603.1_g000021 Rmu_sc0022235.1_g000001 Rmu_sc0042337.1_g000001
rosa_roxburghii	Rroxscaffold_1G00073150
rosa_rugosa	Rorug04G0398800 Rorug04G0398900 Rorug04G0399000
rosa_samantha	Rh5AG022000 Rh5AG022100 Rh5BG024900 Rh5BG025000 Rh5CG024300 Rh5CG024400 Rh5DG023300 Rh5DG023400
rosa_wichuraiana	Rw5G002050 Rw5G002060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	182, 724
AccBSI	CCGCTC	1 cut(s)	891
AccI	GTMKAC	1 cut(s)	1500
AccIII	TCCGGA	1 cut(s)	274
AclWI	GGATC	6 cut(s)	266, 301, 575, 773, 786, 990
AcoI	YGGCCR	1 cut(s)	385
AcsI	RAATTY	3 cut(s)	526, 546, 1102
AcuI	CTGAAG	3 cut(s)	453, 629, 1002
AfaI	GTAC	1 cut(s)	1028
AfiI	CCNNNNNNNGG	6 cut(s)	167, 309, 378, 905, 1194, 1415
AgsI	TTSAA	6 cut(s)	121, 610, 748, 960, 1045, 1378
AjiI	CACGTC	1 cut(s)	253
AjnI	CCWGG	2 cut(s)	377, 1243
AleI	CACNNNNGTG	1 cut(s)	539
AluBI	AGCT	8 cut(s)	18, 24, 133, 340, 473, 618, 1335, 1422
AluI	AGCT	8 cut(s)	18, 24, 133, 340, 473, 618, 1335, 1422
Alw26I	GTCTC	2 cut(s)	210, 1065
AlwI	GGATC	6 cut(s)	266, 301, 575, 773, 786, 990
Aor13HI	TCCGGA	1 cut(s)	274
AoxI	GGCC	4 cut(s)	385, 896, 1077, 1087
ApeKI	GCWGC	8 cut(s)	10, 133, 521, 830, 1220, 1358, 1527, 1530
ApoI	RAATTY	3 cut(s)	526, 546, 1102
ArsI	GACNNNNNNTTYG	4 cut(s)	188, 220, 650, 682
AspLEI	GCGC	1 cut(s)	742
AspS9I	GGNCC	4 cut(s)	145, 214, 1087, 1241
AsuC2I	CCSGG	5 cut(s)	311, 664, 1275, 1363, 1434
AsuHPI	GGTGA	2 cut(s)	1459, 1483
AsuII	TTCGAA	2 cut(s)	194, 902
AvaII	GGWCC	3 cut(s)	145, 214, 1241
BamHI	GGATCC	1 cut(s)	778
BanI	GGYRCC	2 cut(s)	182, 724
BbvI	GCAGC	8 cut(s)	22, 120, 508, 817, 1207, 1345, 1539, 1542
BccI	CCATC	7 cut(s)	356, 766, 888, 1187, 1192, 1196, 1553
BceAI	ACGGC	3 cut(s)	145, 577, 745
BcgI	CGANNNNNNTGC	6 cut(s)	418, 452, 480, 514, 1242, 1276
BciT130I	CCWGG	2 cut(s)	379, 1245
BcnI	CCSGG	5 cut(s)	311, 664, 1275, 1363, 1434
BcoDI	GTCTC	2 cut(s)	210, 1065
BfmI	CTRYAG	1 cut(s)	1585
Bme1390I	CCNGG	7 cut(s)	311, 379, 664, 1245, 1275, 1363, 1434
Bme18I	GGWCC	3 cut(s)	145, 214, 1241
BmgBI	CACGTC	1 cut(s)	253
BmgT120I	GGNCC	4 cut(s)	145, 214, 1087, 1241
BmiI	GGNNCC	5 cut(s)	184, 726, 780, 994, 1088
BmrFI	CCNGG	7 cut(s)	311, 379, 664, 1245, 1275, 1363, 1434
BmsI	GCATC	4 cut(s)	98, 256, 805, 817
BoxI	GACNNNNGTC	1 cut(s)	1410
BpmI	CTGGAG	1 cut(s)	221
Bpu14I	TTCGAA	2 cut(s)	194, 902
BpuEI	CTTGAG	1 cut(s)	4
BpuMI	CCSGG	5 cut(s)	311, 664, 1275, 1363, 1434
BsaAI	YACGTR	1 cut(s)	1129
BsaBI	GATNNNNATC	1 cut(s)	270
BsaI	GGTCTC	1 cut(s)	1065
BsaJI	CCNNGG	5 cut(s)	378, 1074, 1188, 1244, 1274
BsaWI	WCCGGW	1 cut(s)	274
BsaXI	ACNNNNNCTCC	2 cut(s)	91, 121
Bsc4I	CCNNNNNNNGG	6 cut(s)	167, 309, 378, 905, 1194, 1415
Bse1I	ACTGG	3 cut(s)	430, 1067, 1488
Bse3DI	GCAATG	3 cut(s)	429, 638, 1182
Bse8I	GATNNNNATC	1 cut(s)	270
BseAI	TCCGGA	1 cut(s)	274
BseBI	CCWGG	2 cut(s)	379, 1245
BseDI	CCNNGG	5 cut(s)	378, 1074, 1188, 1244, 1274
BseGI	GGATG	7 cut(s)	89, 279, 820, 899, 1198, 1203, 1207
BseJI	GATNNNNATC	1 cut(s)	270
BseLI	CCNNNNNNNGG	6 cut(s)	167, 309, 378, 905, 1194, 1415
BseMI	GCAATG	3 cut(s)	429, 638, 1182
BseMII	CTCAG	1 cut(s)	999
BseNI	ACTGG	3 cut(s)	430, 1067, 1488
BseRI	GAGGAG	4 cut(s)	16, 59, 332, 465
BseX3I	CGGCCG	1 cut(s)	385
BseXI	GCAGC	8 cut(s)	22, 120, 508, 817, 1207, 1345, 1539, 1542
Bsh1285I	CGRYCG	2 cut(s)	388, 438
BshFI	GGCC	4 cut(s)	387, 898, 1079, 1089
BshNI	GGYRCC	2 cut(s)	182, 724
BsiEI	CGRYCG	2 cut(s)	388, 438
BsiSI	CCGG	7 cut(s)	275, 310, 488, 663, 1274, 1362, 1434
BslFI	GGGAC	1 cut(s)	311
BslI	CCNNNNNNNGG	6 cut(s)	167, 309, 378, 905, 1194, 1415
BsmAI	GTCTC	2 cut(s)	210, 1065
BsmFI	GGGAC	1 cut(s)	311
BsnI	GGCC	4 cut(s)	387, 898, 1079, 1089
Bso31I	GGTCTC	1 cut(s)	1065
Bsp119I	TTCGAA	2 cut(s)	194, 902
Bsp13I	TCCGGA	1 cut(s)	274
Bsp1407I	TGTACA	1 cut(s)	1026
Bsp143I	GATC	6 cut(s)	243, 271, 306, 580, 778, 982
Bsp19I	CCATGG	1 cut(s)	1188
BspANI	GGCC	4 cut(s)	387, 898, 1079, 1089
BspCNI	CTCAG	1 cut(s)	1000
BspEI	TCCGGA	1 cut(s)	274
BspLI	GGNNCC	5 cut(s)	184, 726, 780, 994, 1088
BspPI	GGATC	6 cut(s)	266, 301, 575, 773, 786, 990
BspQI	GCTCTTC	1 cut(s)	361
BspT104I	TTCGAA	2 cut(s)	194, 902
BspT107I	GGYRCC	2 cut(s)	182, 724
BspTNI	GGTCTC	1 cut(s)	1065
BsrBI	CCGCTC	1 cut(s)	891
BsrDI	GCAATG	3 cut(s)	429, 638, 1182
BsrGI	TGTACA	1 cut(s)	1026
BsrI	ACTGG	3 cut(s)	430, 1067, 1488
BssECI	CCNNGG	5 cut(s)	378, 1074, 1188, 1244, 1274
BssMI	GATC	6 cut(s)	243, 271, 306, 580, 778, 982
BssT1I	CCWWGG	1 cut(s)	1188
Bst2UI	CCWGG	2 cut(s)	379, 1245
Bst4CI	ACNGT	5 cut(s)	460, 509, 874, 1471, 1589
Bst6I	CTCTTC	2 cut(s)	77, 361
BstAUI	TGTACA	1 cut(s)	1026
BstBAI	YACGTR	1 cut(s)	1129
BstBI	TTCGAA	2 cut(s)	194, 902
BstC8I	GCNNGC	2 cut(s)	1218, 1251
BstDEI	CTNAG	2 cut(s)	45, 1008
BstDSI	CCRYGG	1 cut(s)	1188
BstF5I	GGATG	7 cut(s)	89, 279, 820, 899, 1198, 1203, 1207
BstHHI	GCGC	1 cut(s)	742
BstKTI	GATC	6 cut(s)	246, 274, 309, 583, 781, 985
BstMAI	GTCTC	2 cut(s)	210, 1065
BstMBI	GATC	6 cut(s)	243, 271, 306, 580, 778, 982
BstMCI	CGRYCG	2 cut(s)	388, 438
BstMWI	GCNNNNNNNGC	6 cut(s)	95, 238, 518, 748, 757, 1322
BstNI	CCWGG	2 cut(s)	379, 1245
BstNSI	RCATGY	2 cut(s)	977, 1239
BstPAI	GACNNNNGTC	1 cut(s)	1410
BstSCI	CCNGG	7 cut(s)	309, 377, 662, 1243, 1273, 1361, 1432
BstSFI	CTRYAG	1 cut(s)	1585
BstV1I	GCAGC	8 cut(s)	22, 120, 508, 817, 1207, 1345, 1539, 1542
BstX2I	RGATCY	2 cut(s)	306, 778
BstYI	RGATCY	2 cut(s)	306, 778
BstZI	CGGCCG	1 cut(s)	385
BsuRI	GGCC	4 cut(s)	387, 898, 1079, 1089
BtgI	CCRYGG	1 cut(s)	1188
BtrI	CACGTC	1 cut(s)	253
BtsCI	GGATG	7 cut(s)	89, 279, 820, 899, 1198, 1203, 1207
BtsIMutI	CAGTG	4 cut(s)	423, 514, 1074, 1401
Cac8I	GCNNGC	2 cut(s)	1218, 1251
CfoI	GCGC	1 cut(s)	742
Cfr13I	GGNCC	4 cut(s)	145, 214, 1087, 1241
CseI	GACGC	1 cut(s)	313
Csp6I	GTAC	1 cut(s)	1027
CviAII	CATG	8 cut(s)	539, 820, 844, 974, 1189, 1236, 1313, 1617
CviQI	GTAC	1 cut(s)	1027
DdeI	CTNAG	2 cut(s)	45, 1008
DpnI	GATC	6 cut(s)	245, 273, 308, 582, 780, 984
DpnII	GATC	6 cut(s)	243, 271, 306, 580, 778, 982
EaeI	YGGCCR	1 cut(s)	385
EagI	CGGCCG	1 cut(s)	385
Eam1104I	CTCTTC	2 cut(s)	77, 361
EarI	CTCTTC	2 cut(s)	77, 361
EciI	GGCGGA	3 cut(s)	245, 579, 1053
EclXI	CGGCCG	1 cut(s)	385
Eco130I	CCWWGG	1 cut(s)	1188
Eco31I	GGTCTC	1 cut(s)	1065
Eco47I	GGWCC	3 cut(s)	145, 214, 1241
Eco52I	CGGCCG	1 cut(s)	385
Eco57I	CTGAAG	3 cut(s)	453, 629, 1002
EcoO109I	RGGNCCY	1 cut(s)	1087
EcoRI	GAATTC	1 cut(s)	1102
EcoRII	CCWGG	2 cut(s)	377, 1243
EcoT14I	CCWWGG	1 cut(s)	1188
EcoT22I	ATGCAT	1 cut(s)	1327
ErhI	CCWWGG	1 cut(s)	1188
FaeI	CATG	8 cut(s)	542, 823, 847, 977, 1192, 1239, 1316, 1620
FalI	AAGNNNNNCTT	2 cut(s)	1300, 1332
FaqI	GGGAC	1 cut(s)	311
FatI	CATG	8 cut(s)	538, 819, 843, 973, 1188, 1235, 1312, 1616
FauI	CCCGC	1 cut(s)	1242
FauNDI	CATATG	1 cut(s)	1321
FblI	GTMKAC	1 cut(s)	1500
FokI	GGATG	7 cut(s)	76, 266, 827, 906, 1205, 1210, 1214
GlaI	GCGC	1 cut(s)	741
GsuI	CTGGAG	1 cut(s)	221
HaeIII	GGCC	4 cut(s)	387, 898, 1079, 1089
HapII	CCGG	7 cut(s)	275, 310, 488, 663, 1274, 1362, 1434
HgaI	GACGC	1 cut(s)	313
HhaI	GCGC	1 cut(s)	742
Hin1II	CATG	8 cut(s)	542, 823, 847, 977, 1192, 1239, 1316, 1620
Hin6I	GCGC	1 cut(s)	740
HinP1I	GCGC	1 cut(s)	740
HincII	GTYRAC	1 cut(s)	453
HindII	GTYRAC	1 cut(s)	453
HindIII	AAGCTT	1 cut(s)	616
HinfI	GANTC	5 cut(s)	196, 682, 911, 1267, 1612
HpaI	GTTAAC	1 cut(s)	453
HpaII	CCGG	7 cut(s)	275, 310, 488, 663, 1274, 1362, 1434
HphI	GGTGA	2 cut(s)	1459, 1483
Hpy166II	GTNNAC	6 cut(s)	175, 453, 1027, 1241, 1474, 1501
Hpy188I	TCNGA	7 cut(s)	170, 214, 886, 1021, 1075, 1264, 1296
Hpy188III	TCNNGA	4 cut(s)	143, 275, 578, 731
Hpy8I	GTNNAC	6 cut(s)	175, 453, 1027, 1241, 1474, 1501
Hpy99I	CGWCG	7 cut(s)	170, 191, 214, 398, 401, 446, 766
HpyAV	CCTTC	5 cut(s)	474, 898, 909, 1438, 1531
HpyCH4III	ACNGT	5 cut(s)	460, 509, 874, 1471, 1589
HpyCH4IV	ACGT	4 cut(s)	189, 252, 393, 1128
HpyF10VI	GCNNNNNNNGC	6 cut(s)	95, 238, 518, 748, 757, 1322
HpyF3I	CTNAG	2 cut(s)	45, 1008
HpySE526I	ACGT	4 cut(s)	189, 252, 393, 1128
Hsp92II	CATG	8 cut(s)	542, 823, 847, 977, 1192, 1239, 1316, 1620
HspAI	GCGC	1 cut(s)	740
Kpn2I	TCCGGA	1 cut(s)	274
KspAI	GTTAAC	1 cut(s)	453
Kzo9I	GATC	6 cut(s)	243, 271, 306, 580, 778, 982
LguI	GCTCTTC	1 cut(s)	361
LmnI	GCTCC	4 cut(s)	29, 345, 478, 1419
Lsp1109I	GCAGC	8 cut(s)	22, 120, 508, 817, 1207, 1345, 1539, 1542
LweI	GCATC	4 cut(s)	98, 256, 805, 817
MaeII	ACGT	4 cut(s)	189, 252, 393, 1128
MaeIII	GTNAC	7 cut(s)	40, 594, 868, 1286, 1370, 1412, 1609
MalI	GATC	6 cut(s)	245, 273, 308, 582, 780, 984
MbiI	CCGCTC	1 cut(s)	891
MboI	GATC	6 cut(s)	243, 271, 306, 580, 778, 982
MfeI	CAATTG	1 cut(s)	1081
MflI	RGATCY	2 cut(s)	306, 778
MlyI	GAGTC	1 cut(s)	1606
MmeI	TCCRAC	3 cut(s)	148, 192, 384
Mph1103I	ATGCAT	1 cut(s)	1327
MroI	TCCGGA	1 cut(s)	274
MseI	TTAA	4 cut(s)	452, 620, 855, 1508
MslI	CAYNNNNRTG	3 cut(s)	537, 539, 1187
MspA1I	CMGCKG	1 cut(s)	673
MspI	CCGG	7 cut(s)	275, 310, 488, 663, 1274, 1362, 1434
MspR9I	CCNGG	7 cut(s)	311, 379, 664, 1245, 1275, 1363, 1434
MunI	CAATTG	1 cut(s)	1081
MvaI	CCWGG	2 cut(s)	379, 1245
MwoI	GCNNNNNNNGC	6 cut(s)	95, 238, 518, 748, 757, 1322
NciI	CCSGG	5 cut(s)	311, 664, 1275, 1363, 1434
NcoI	CCATGG	1 cut(s)	1188
NdeI	CATATG	1 cut(s)	1321
NdeII	GATC	6 cut(s)	243, 271, 306, 580, 778, 982
NlaIII	CATG	8 cut(s)	542, 823, 847, 977, 1192, 1239, 1316, 1620
NlaIV	GGNNCC	5 cut(s)	184, 726, 780, 994, 1088
NmuCI	GTSAC	6 cut(s)	40, 594, 868, 1370, 1412, 1609
NsiI	ATGCAT	1 cut(s)	1327
NspI	RCATGY	2 cut(s)	977, 1239
NspV	TTCGAA	2 cut(s)	194, 902
OliI	CACNNNNGTG	1 cut(s)	539
PciSI	GCTCTTC	1 cut(s)	361
PcsI	WCGNNNNNNNCGW	3 cut(s)	321, 390, 1475
PfeI	GAWTC	4 cut(s)	196, 682, 911, 1267
PfoI	TCCNGGA	1 cut(s)	1432
PleI	GAGTC	1 cut(s)	1606
PpsI	GAGTC	1 cut(s)	1606
Ppu21I	YACGTR	1 cut(s)	1129
PshAI	GACNNNNGTC	1 cut(s)	1410
Psp6I	CCWGG	2 cut(s)	377, 1243
PspGI	CCWGG	2 cut(s)	377, 1243
PspN4I	GGNNCC	5 cut(s)	184, 726, 780, 994, 1088
PspPI	GGNCC	4 cut(s)	145, 214, 1087, 1241
PsuI	RGATCY	2 cut(s)	306, 778
RsaI	GTAC	1 cut(s)	1028
RsaNI	GTAC	1 cut(s)	1027
RseI	CAYNNNNRTG	3 cut(s)	537, 539, 1187
SapI	GCTCTTC	1 cut(s)	361
SaqAI	TTAA	4 cut(s)	452, 620, 855, 1508
Sau3AI	GATC	6 cut(s)	243, 271, 306, 580, 778, 982
Sau96I	GGNCC	4 cut(s)	145, 214, 1087, 1241
SchI	GAGTC	1 cut(s)	1606
ScrFI	CCNGG	7 cut(s)	311, 379, 664, 1245, 1275, 1363, 1434
SfaNI	GCATC	4 cut(s)	98, 256, 805, 817
SfcI	CTRYAG	1 cut(s)	1585
SfuI	TTCGAA	2 cut(s)	194, 902
SinI	GGWCC	3 cut(s)	145, 214, 1241
SmiMI	CAYNNNNRTG	3 cut(s)	537, 539, 1187
SmlI	CTYRAG	1 cut(s)	19
SmoI	CTYRAG	1 cut(s)	19
SspI	AATATT	1 cut(s)	859
StyD4I	CCNGG	7 cut(s)	309, 377, 662, 1243, 1273, 1361, 1432
StyI	CCWWGG	1 cut(s)	1188
TaaI	ACNGT	5 cut(s)	460, 509, 874, 1471, 1589
TaiI	ACGT	4 cut(s)	192, 255, 396, 1131
TatI	WGTACW	1 cut(s)	1026
TauI	GCSGC	3 cut(s)	374, 566, 673
TfiI	GAWTC	4 cut(s)	196, 682, 911, 1267
Tru1I	TTAA	4 cut(s)	452, 620, 855, 1508
Tru9I	TTAA	4 cut(s)	452, 620, 855, 1508
TscAI	CASTG	4 cut(s)	430, 514, 1074, 1408
TseFI	GTSAC	6 cut(s)	40, 594, 868, 1370, 1412, 1609
TseI	GCWGC	8 cut(s)	10, 133, 521, 830, 1220, 1358, 1527, 1530
Tsp45I	GTSAC	6 cut(s)	40, 594, 868, 1370, 1412, 1609
TspDTI	ATGAA	4 cut(s)	695, 860, 1050, 1308
TspGWI	ACGGA	3 cut(s)	198, 573, 1552
TspRI	CASTG	4 cut(s)	430, 514, 1074, 1408
VpaK11BI	GGWCC	3 cut(s)	145, 214, 1241
XapI	RAATTY	3 cut(s)	526, 546, 1102
XceI	RCATGY	2 cut(s)	977, 1239
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1195
XmiI	GTMKAC	1 cut(s)	1500
Zsp2I	ATGCAT	1 cut(s)	1327

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.