Rh5DG023300

Tyrosyl-DNA phosphodiesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003730
Physical Location & Seq
Reverse (-)
1642551 .. 1646219
3669 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG023300.1

Sequence Viewer

Length: 1422 bp
ATGTCCTGGCAATGCAAAAACTGCACCTTTCTCAACCCCAAAACCCCCTCTTTACCAAAACCCACTTGCCAAATCTGCCTATCTTCACCTCCCCAATCCCCATCTTCATCTTCCTCCTCTGCCTCAAAATGGGCCTGCAAGGCCTGCACTTTCTTGAACCCTTACAAGCGCTCCAGCTGTGAGGTCTGTGACACAAGGGCCCCAATCTCGAGCTTCGAGGATCTGAATGATGACAATGGAGATGAGCTTGATGATCAAGTGGGCTCTGTTTTCTTGCCCTTGCAGAAGTGTAAGAGGAAGAGGGTTGAGGAAGATGAGGGTTCTGGGAATGGGTTTAAGGGGAGGAGGGTTCAAGACCCACTTGAAGTTGCTAAGGAAATTACTGTATCAGACCCAGTTCAGGTTGCTAAGGATTCTGCCACATTGAATGCGTTTAGAGGAGTCAAGGTGTCTGATAAGAAGAAGATCACTGTATCTGAGAGCACTAGTTCAGTTGAGTCTGTAAAGGATGATGCCAAATTCAGTTTGTTTCGAGGAGTCAAGGTGTCTGATAAGAAGGTTACTGTATCAGAGAGCAGTAGTTCCGGGTCTTCTGGAATGGGTTCGAATACGCTCAAGGTTTTGACTTACAATGTTTGGTTTCGAGAAGACTTAGAAATGCACAAGAGAATGAAAGCTATTGGTGACCTTGTCCTACAGCATTGCCCAGATGTCATTTGCTTTCAGGAGGTTACCCCCGATATATACGACATCTTTCAACAATCTAACTGGTGGAAAATGTATCAATGCTCAATTCCAAATCAGATGGCGATTTCACAACCTTACTTTTGCATGCAGTTAAGCAAATTGCAAGTGAAAGCCTTCAGCTGTAGACCATTTTCATACTCTGCGATGGGGAGAGAACTCTGTGTCACTGAAGTTGAAGTACAGGGGGACAAACGTCTTGTTGTTGCCACTAGCCATCTTGAGAGTCCTTGCCCAGGACCTCCAAAATGGGATCAGATGTACAGCAAGGAACGTGTAGATCAGGCAAAGGAGGCCCTCAACTTCTTAAAGAAGAACCCAAATGTGATTTTTTGCGGTGACATGAACTGGGATGACAAGTTGGATGGCCAAGTTCCTTTACCTGACAACTGGATTGATGCTTGGGTAGAGTTACGACCAGGGGAAGATGGATTGACGTATGATACCAAGTCCAACCAGATGTTGTCTGGTAACAGGTCACTGCGGAAGCGACTAGATCGATTCTTTTGCTGTCTGCACGATTTCAGGGTGAGCAAAATTGAAATGATTGGGACGAAAGCAATACCAGGTCTCTCATATATCAAGGAAAAGAAAGTGAAAGGGGAGATTAAGAAGCTGGAGCTCCCTGTTTTGCCTAGCGATCATTATGGCTTGCTTTTGACAATCTGTGGCCAGTAG

Protein Analysis

473

Amino Acids

53.13

Weight (kDa)

8.13

Isoelectric Point (pI)

48.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 208 - 463 4e-17 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 871
AciI CCGC 2 cut(s) 1080, 1228
AclWI GGATC 2 cut(s) 228, 1005
AcoI YGGCCR 2 cut(s) 1111, 1414
AcsI RAATTY 1 cut(s) 518
AcuI CTGAAG 2 cut(s) 847, 936
AfaI GTAC 2 cut(s) 927, 1007
AfeI AGCGCT 1 cut(s) 170
AfiI CCNNNNNNNGG 3 cut(s) 129, 400, 980
AflIII ACRYGT 1 cut(s) 1018
AgsI TTSAA 7 cut(s) 157, 353, 365, 427, 758, 923, 1286
AhlI ACTAGT 1 cut(s) 485
AjnI CCWGG 4 cut(s) 5, 979, 1162, 1309
AluBI AGCT 7 cut(s) 177, 213, 247, 677, 867, 1360, 1366
AluI AGCT 7 cut(s) 177, 213, 247, 677, 867, 1360, 1366
Alw21I GWGCWC 2 cut(s) 485, 1368
Alw26I GTCTC 1 cut(s) 1319
AlwI GGATC 2 cut(s) 228, 1005
Ama87I CYCGRG 1 cut(s) 208
Aor51HI AGCGCT 1 cut(s) 170
AoxI GGCC 6 cut(s) 132, 141, 198, 1038, 1111, 1414
ApaI GGGCCC 1 cut(s) 202
ApoI RAATTY 1 cut(s) 518
Asp700I GAANNNNTTC 2 cut(s) 601, 860
AspLEI GCGC 1 cut(s) 171
AspS9I GGNCC 5 cut(s) 132, 198, 199, 983, 1039
AsuC2I CCSGG 1 cut(s) 586
AsuHPI GGTGA 4 cut(s) 78, 695, 1094, 1285
AsuII TTCGAA 1 cut(s) 605
AvaI CYCGRG 1 cut(s) 208
AvaII GGWCC 1 cut(s) 983
BaeGI GKGCMC 1 cut(s) 202
BalI TGGCCA 2 cut(s) 1113, 1416
BanII GRGCYC 3 cut(s) 202, 266, 1368
BarI GAAGNNNNNNTAC 2 cut(s) 909, 941
BbsI GAAGAC 2 cut(s) 582, 654
Bbv12I GWGCWC 2 cut(s) 485, 1368
BccI CCATC 6 cut(s) 109, 799, 886, 969, 1103, 1166
BcgI CGANNNNNNTGC 2 cut(s) 1233, 1267
BciT130I CCWGG 4 cut(s) 7, 981, 1164, 1311
BclI TGATCA 1 cut(s) 253
BcnI CCSGG 1 cut(s) 586
BcoDI GTCTC 1 cut(s) 1319
BcuI ACTAGT 1 cut(s) 485
BfaI CTAG 4 cut(s) 486, 957, 1238, 1380
BfmI CTRYAG 2 cut(s) 695, 868
BfoI RGCGCY 1 cut(s) 172
BglI GCCNNNNNGGC 1 cut(s) 140
Bme1390I CCNGG 5 cut(s) 7, 586, 981, 1164, 1311
Bme18I GGWCC 1 cut(s) 983
BmeT110I CYCGRG 1 cut(s) 208
BmgT120I GGNCC 5 cut(s) 132, 198, 199, 983, 1039
BmiI GGNNCC 2 cut(s) 200, 201
BmrFI CCNGG 5 cut(s) 7, 586, 981, 1164, 1311
BmrI ACTGGG 2 cut(s) 389, 1102
BmsI GCATC 2 cut(s) 502, 1132
BmuI ACTGGG 2 cut(s) 389, 1102
BoxI GACNNNNGTC 1 cut(s) 939
BpiI GAAGAC 2 cut(s) 582, 654
BpmI CTGGAG 2 cut(s) 157, 1382
Bpu10I CCTNAGC 2 cut(s) 372, 408
Bpu14I TTCGAA 1 cut(s) 605
BpuEI CTTGAG 2 cut(s) 599, 986
BpuMI CCSGG 1 cut(s) 586
Bsa29I ATCGAT 1 cut(s) 1243
BsaI GGTCTC 1 cut(s) 1319
BsaJI CCNNGG 2 cut(s) 979, 1163
BsaXI ACNNNNNCTCC 6 cut(s) 155, 185, 432, 462, 528, 558
Bsc4I CCNNNNNNNGG 3 cut(s) 129, 400, 980
Bse1I ACTGG 5 cut(s) 395, 773, 1097, 1139, 1417
Bse3DI GCAATG 2 cut(s) 17, 700
BseBI CCWGG 4 cut(s) 7, 981, 1164, 1311
BseCI ATCGAT 1 cut(s) 1243
BseDI CCNNGG 2 cut(s) 979, 1163
BseGI GGATG 3 cut(s) 514, 1102, 1114
BseLI CCNNNNNNNGG 3 cut(s) 129, 400, 980
BseMI GCAATG 2 cut(s) 17, 700
BseMII CTCAG 1 cut(s) 468
BseNI ACTGG 5 cut(s) 395, 773, 1097, 1139, 1417
BseRI GAGGAG 4 cut(s) 106, 358, 453, 549
BseSI GKGCMC 1 cut(s) 202
BsgI GTGCAG 3 cut(s) 7, 130, 1244
BshFI GGCC 6 cut(s) 134, 143, 200, 1040, 1113, 1416
BshVI ATCGAT 1 cut(s) 1243
BsiHKAI GWGCWC 2 cut(s) 485, 1368
BsiHKCI CYCGRG 1 cut(s) 208
BsiSI CCGG 1 cut(s) 585
BslFI GGGAC 2 cut(s) 947, 1309
BslI CCNNNNNNNGG 3 cut(s) 129, 400, 980
BsmAI GTCTC 1 cut(s) 1319
BsmFI GGGAC 2 cut(s) 947, 1309
BsmI GAATGC 1 cut(s) 433
BsnI GGCC 6 cut(s) 134, 143, 200, 1040, 1113, 1416
Bso31I GGTCTC 1 cut(s) 1319
BsoBI CYCGRG 1 cut(s) 208
Bsp119I TTCGAA 1 cut(s) 605
Bsp120I GGGCCC 1 cut(s) 198
Bsp1286I GDGCHC 4 cut(s) 202, 266, 485, 1368
Bsp1407I TGTACA 1 cut(s) 1005
Bsp143I GATC 7 cut(s) 220, 253, 465, 997, 1024, 1240, 1384
BspACI CCGC 2 cut(s) 1080, 1228
BspANI GGCC 6 cut(s) 134, 143, 200, 1040, 1113, 1416
BspCNI CTCAG 1 cut(s) 469
BspDI ATCGAT 1 cut(s) 1243
BspLI GGNNCC 2 cut(s) 200, 201
BspPI GGATC 2 cut(s) 228, 1005
BspT104I TTCGAA 1 cut(s) 605
BspTNI GGTCTC 1 cut(s) 1319
BsrDI GCAATG 2 cut(s) 17, 700
BsrGI TGTACA 1 cut(s) 1005
BsrI ACTGG 5 cut(s) 395, 773, 1097, 1139, 1417
BssECI CCNNGG 2 cut(s) 979, 1163
BssMI GATC 7 cut(s) 220, 253, 465, 997, 1024, 1240, 1384
Bst2UI CCWGG 4 cut(s) 7, 981, 1164, 1311
Bst4CI ACNGT 3 cut(s) 385, 472, 565
Bst6I CTCTTC 1 cut(s) 293
BstAPI GCANNNNNTGC 2 cut(s) 21, 144
BstAUI TGTACA 1 cut(s) 1005
BstBI TTCGAA 1 cut(s) 605
BstC8I GCNNGC 4 cut(s) 136, 145, 833, 1397
BstDEI CTNAG 4 cut(s) 372, 408, 477, 652
BstEII GGTNACC 2 cut(s) 683, 730
BstENI CCTNNNNNAGG 1 cut(s) 978
BstF5I GGATG 3 cut(s) 514, 1102, 1114
BstH2I RGCGCY 1 cut(s) 172
BstHHI GCGC 1 cut(s) 171
BstKTI GATC 7 cut(s) 223, 256, 468, 1000, 1027, 1243, 1387
BstMAI GTCTC 1 cut(s) 1319
BstMBI GATC 7 cut(s) 220, 253, 465, 997, 1024, 1240, 1384
BstMWI GCNNNNNNNGC 5 cut(s) 21, 75, 140, 144, 1037
BstNI CCWGG 4 cut(s) 7, 981, 1164, 1311
BstNSI RCATGY 1 cut(s) 835
BstPAI GACNNNNGTC 1 cut(s) 939
BstPI GGTNACC 2 cut(s) 683, 730
BstSCI CCNGG 5 cut(s) 5, 584, 979, 1162, 1309
BstSFI CTRYAG 2 cut(s) 695, 868
BstSLI GKGCMC 1 cut(s) 202
BstV2I GAAGAC 2 cut(s) 582, 654
BstX2I RGATCY 1 cut(s) 220
BstYI RGATCY 1 cut(s) 220
Bsu15I ATCGAT 1 cut(s) 1243
BsuRI GGCC 6 cut(s) 134, 143, 200, 1040, 1113, 1416
BsuTUI ATCGAT 1 cut(s) 1243
BtgZI GCGATG 1 cut(s) 905
BtsCI GGATG 3 cut(s) 514, 1102, 1114
BtsI GCAGTG 1 cut(s) 1223
BtsIMutI CAGTG 3 cut(s) 468, 912, 1223
Cac8I GCNNGC 4 cut(s) 136, 145, 833, 1397
CfoI GCGC 1 cut(s) 171
Cfr13I GGNCC 5 cut(s) 132, 198, 199, 983, 1039
ClaI ATCGAT 1 cut(s) 1243
CsiI ACCWGGT 1 cut(s) 1309
Csp6I GTAC 2 cut(s) 926, 1006
CviAII CATG 2 cut(s) 832, 1087
CviQI GTAC 2 cut(s) 926, 1006
DdeI CTNAG 4 cut(s) 372, 408, 477, 652
DpnI GATC 7 cut(s) 222, 255, 467, 999, 1026, 1242, 1386
DpnII GATC 7 cut(s) 220, 253, 465, 997, 1024, 1240, 1384
EaeI YGGCCR 2 cut(s) 1111, 1414
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
Ecl136II GAGCTC 1 cut(s) 1366
Eco147I AGGCCT 1 cut(s) 143
Eco24I GRGCYC 3 cut(s) 202, 266, 1368
Eco31I GGTCTC 1 cut(s) 1319
Eco47I GGWCC 1 cut(s) 983
Eco47III AGCGCT 1 cut(s) 170
Eco53kI GAGCTC 1 cut(s) 1366
Eco57I CTGAAG 2 cut(s) 847, 936
Eco88I CYCGRG 1 cut(s) 208
Eco91I GGTNACC 2 cut(s) 683, 730
EcoICRI GAGCTC 1 cut(s) 1366
EcoNI CCTNNNNNAGG 1 cut(s) 978
EcoO109I RGGNCCY 4 cut(s) 198, 199, 983, 1039
EcoO65I GGTNACC 2 cut(s) 683, 730
EcoRII CCWGG 4 cut(s) 5, 979, 1162, 1309
EcoT38I GRGCYC 3 cut(s) 202, 266, 1368
FaeI CATG 2 cut(s) 835, 1090
FaiI YATR 9 cut(s) 743, 745, 833, 883, 1088, 1185, 1321, 1323, 1392
FalI AAGNNNNNCTT 2 cut(s) 345, 377
FaqI GGGAC 2 cut(s) 947, 1309
FatI CATG 2 cut(s) 831, 1086
FbaI TGATCA 1 cut(s) 253
FblI GTMKAC 1 cut(s) 871
FokI GGATG 3 cut(s) 521, 1109, 1121
FriOI GRGCYC 3 cut(s) 202, 266, 1368
FspBI CTAG 4 cut(s) 486, 957, 1238, 1380
GlaI GCGC 1 cut(s) 170
GsuI CTGGAG 2 cut(s) 157, 1382
HaeII RGCGCY 1 cut(s) 172
HaeIII GGCC 6 cut(s) 134, 143, 200, 1040, 1113, 1416
HapII CCGG 1 cut(s) 585
HhaI GCGC 1 cut(s) 171
Hin1II CATG 2 cut(s) 835, 1090
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HinfI GANTC 6 cut(s) 413, 441, 497, 537, 970, 1245
HpaII CCGG 1 cut(s) 585
HphI GGTGA 4 cut(s) 78, 695, 1094, 1285
Hpy166II GTNNAC 1 cut(s) 872
Hpy188I TCNGA 8 cut(s) 225, 391, 454, 478, 550, 571, 804, 1002
Hpy188III TCNNGA 7 cut(s) 154, 208, 353, 594, 644, 725, 965
Hpy8I GTNNAC 1 cut(s) 872
HpyAV CCTTC 2 cut(s) 550, 871
HpyCH4III ACNGT 3 cut(s) 385, 472, 565
HpyCH4IV ACGT 3 cut(s) 940, 1018, 1181
HpyF10VI GCNNNNNNNGC 5 cut(s) 21, 75, 140, 144, 1037
HpyF3I CTNAG 4 cut(s) 372, 408, 477, 652
HpySE526I ACGT 3 cut(s) 940, 1018, 1181
Hsp92II CATG 2 cut(s) 835, 1090
HspAI GCGC 1 cut(s) 169
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 7 cut(s) 220, 253, 465, 997, 1024, 1240, 1384
LmnI GCTCC 3 cut(s) 176, 1363, 1371
LweI GCATC 2 cut(s) 502, 1132
MabI ACCWGGT 1 cut(s) 1309
MaeI CTAG 4 cut(s) 486, 957, 1238, 1380
MaeII ACGT 3 cut(s) 940, 1018, 1181
MaeIII GTNAC 9 cut(s) 188, 559, 683, 730, 910, 1082, 1155, 1214, 1221
MalI GATC 7 cut(s) 222, 255, 467, 999, 1026, 1242, 1386
MboI GATC 7 cut(s) 220, 253, 465, 997, 1024, 1240, 1384
MflI RGATCY 1 cut(s) 220
MhlI GDGCHC 4 cut(s) 202, 266, 485, 1368
MlsI TGGCCA 2 cut(s) 1113, 1416
MluCI AATT 5 cut(s) 378, 518, 792, 845, 1281
MluNI TGGCCA 2 cut(s) 1113, 1416
MlyI GAGTC 4 cut(s) 450, 506, 546, 979
MmeI TCCRAC 2 cut(s) 1086, 1221
Mox20I TGGCCA 2 cut(s) 1113, 1416
MroXI GAANNNNTTC 2 cut(s) 601, 860
MscI TGGCCA 2 cut(s) 1113, 1416
MseI TTAA 4 cut(s) 336, 839, 1052, 1353
Msp20I TGGCCA 2 cut(s) 1113, 1416
MspA1I CMGCKG 2 cut(s) 177, 867
MspI CCGG 1 cut(s) 585
MspR9I CCNGG 5 cut(s) 7, 586, 981, 1164, 1311
Mva1269I GAATGC 1 cut(s) 433
MvaI CCWGG 4 cut(s) 7, 981, 1164, 1311
MwoI GCNNNNNNNGC 5 cut(s) 21, 75, 140, 144, 1037
NciI CCSGG 1 cut(s) 586
NdeII GATC 7 cut(s) 220, 253, 465, 997, 1024, 1240, 1384
NlaIII CATG 2 cut(s) 835, 1090
NlaIV GGNNCC 2 cut(s) 200, 201
NmuCI GTSAC 5 cut(s) 188, 683, 910, 1082, 1221
NspI RCATGY 1 cut(s) 835
NspV TTCGAA 1 cut(s) 605
PaeI GCATGC 1 cut(s) 835
PaeR7I CTCGAG 1 cut(s) 208
PceI AGGCCT 1 cut(s) 143
PctI GAATGC 1 cut(s) 433
PdmI GAANNNNTTC 2 cut(s) 601, 860
PfeI GAWTC 2 cut(s) 413, 1245
PflFI GACNNNGTC 1 cut(s) 689
PleI GAGTC 4 cut(s) 449, 505, 545, 978
PpsI GAGTC 4 cut(s) 449, 505, 545, 978
PpuMI RGGWCCY 1 cut(s) 983
PshAI GACNNNNGTC 1 cut(s) 939
Psp124BI GAGCTC 1 cut(s) 1368
Psp5II RGGWCCY 1 cut(s) 983
Psp6I CCWGG 4 cut(s) 5, 979, 1162, 1309
PspEI GGTNACC 2 cut(s) 683, 730
PspGI CCWGG 4 cut(s) 5, 979, 1162, 1309
PspN4I GGNNCC 2 cut(s) 200, 201
PspOMI GGGCCC 1 cut(s) 198
PspPI GGNCC 5 cut(s) 132, 198, 199, 983, 1039
PspPPI RGGWCCY 1 cut(s) 983
PsuI RGATCY 1 cut(s) 220
PsyI GACNNNGTC 1 cut(s) 689
PvuII CAGCTG 2 cut(s) 177, 867
RsaI GTAC 2 cut(s) 927, 1007
RsaNI GTAC 2 cut(s) 926, 1006
SacI GAGCTC 1 cut(s) 1368
SaqAI TTAA 4 cut(s) 336, 839, 1052, 1353
Sau3AI GATC 7 cut(s) 220, 253, 465, 997, 1024, 1240, 1384
Sau96I GGNCC 5 cut(s) 132, 198, 199, 983, 1039
SchI GAGTC 4 cut(s) 450, 506, 546, 979
ScrFI CCNGG 5 cut(s) 7, 586, 981, 1164, 1311
SduI GDGCHC 4 cut(s) 202, 266, 485, 1368
SexAI ACCWGGT 1 cut(s) 1309
SfaNI GCATC 2 cut(s) 502, 1132
SfcI CTRYAG 2 cut(s) 695, 868
SfiI GGCCNNNNNGGCC 1 cut(s) 140
Sfr274I CTCGAG 1 cut(s) 208
SfuI TTCGAA 1 cut(s) 605
SinI GGWCC 1 cut(s) 983
SlaI CTCGAG 1 cut(s) 208
SmlI CTYRAG 3 cut(s) 208, 614, 965
SmoI CTYRAG 3 cut(s) 208, 614, 965
SpeI ACTAGT 1 cut(s) 485
SphI GCATGC 1 cut(s) 835
Sse9I AATT 5 cut(s) 378, 518, 792, 845, 1281
SseBI AGGCCT 1 cut(s) 143
SsiI CCGC 2 cut(s) 1080, 1228
SspMI CTAG 4 cut(s) 486, 957, 1238, 1380
SstI GAGCTC 1 cut(s) 1368
StuI AGGCCT 1 cut(s) 143
StyD4I CCNGG 5 cut(s) 5, 584, 979, 1162, 1309
TaaI ACNGT 3 cut(s) 385, 472, 565
TaiI ACGT 3 cut(s) 943, 1021, 1184
TaqI TCGA 6 cut(s) 209, 216, 532, 605, 643, 1243
TasI AATT 5 cut(s) 378, 518, 792, 845, 1281
TatI WGTACW 2 cut(s) 925, 1005
TfiI GAWTC 2 cut(s) 413, 1245
Tru1I TTAA 4 cut(s) 336, 839, 1052, 1353
Tru9I TTAA 4 cut(s) 336, 839, 1052, 1353
TscAI CASTG 3 cut(s) 475, 919, 1230
TseFI GTSAC 5 cut(s) 188, 683, 910, 1082, 1221
Tsp45I GTSAC 5 cut(s) 188, 683, 910, 1082, 1221
TspDTI ATGAA 4 cut(s) 96, 686, 870, 1103
TspRI CASTG 3 cut(s) 475, 919, 1230
Tth111I GACNNNGTC 1 cut(s) 689
VpaK11BI GGWCC 1 cut(s) 983
XagI CCTNNNNNAGG 1 cut(s) 978
XapI RAATTY 1 cut(s) 518
XceI RCATGY 1 cut(s) 835
XcmI CCANNNNNNNNNTGG 1 cut(s) 1208
XhoI CTCGAG 1 cut(s) 208
XmiI GTMKAC 1 cut(s) 871
XmnI GAANNNNTTC 2 cut(s) 601, 860
XspI CTAG 4 cut(s) 486, 957, 1238, 1380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.