MD06G1006000.v1.1

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0010571
Physical Location & Seq
Reverse (-)
630908 .. 631671
764 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1006000.v1.1.491

Sequence Viewer

Length: 525 bp
ATGCAGTCTGAATTTGGGTTCACATCATGCTTCCACGACAACTTGCCTCTGATATTGACAAGGGTTTTCCTTGGAGTAGCCCTACAGATCCTATGCAGTTACATTACCTTCCCTCTTTATTGTTTGATTACACAAATGGGATCTCATATGAAGAAAGCAATTTTTGAGGAGCAAACGGCAAAGGCTCTTAAGAACTGGCAAAAGGCTGCAAAGCAAAGAAAGAAGTTGAGGAATACTAAAGGAGATGCTTCTTCCTCAGGATTTGTAAGCGGGGAAAATACGCCAAGTCGGGGTACATCACCCTTGCATCTGCTTCACAACCAAAAGCTCAGGTCCAATCAATCCGACCTTGAAAGCGTCCTCAATTCCCCAAGATCTTACCAATCTGACGCCGAGCTCTCGGAGATGGAAGGCTCCGCACATGACGGGAATGGTTTTAGAAGACTAGGAGACCAACCTGCAACAGCAATAGAAATGAAAGAAGGATCACATACTGTTGATTTTACATTTGCTAGTCGACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

175

Amino Acids

19.34

Weight (kDa)

8.81

Isoelectric Point (pI)

54.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 3 - 108 1.9e-37 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 466
AccI GTMKAC 1 cut(s) 517
AciI CCGC 2 cut(s) 270, 417
AclWI GGATC 3 cut(s) 82, 148, 493
AcsI RAATTY 1 cut(s) 11
AcyI GRCGYC 1 cut(s) 390
AfaI GTAC 1 cut(s) 295
AfiI CCNNNNNNNGG 1 cut(s) 290
AflII CTTAAG 1 cut(s) 188
AgsI TTSAA 1 cut(s) 353
AluBI AGCT 2 cut(s) 328, 397
AluI AGCT 2 cut(s) 328, 397
Alw21I GWGCWC 1 cut(s) 399
Alw26I GTCTC 1 cut(s) 444
AlwI GGATC 3 cut(s) 82, 148, 493
ApeKI GCWGC 1 cut(s) 206
ApoI RAATTY 1 cut(s) 11
AspS9I GGNCC 1 cut(s) 333
AsuHPI GGTGA 1 cut(s) 291
AvaII GGWCC 1 cut(s) 333
AxyI CCTNAGG 1 cut(s) 256
BanII GRGCYC 1 cut(s) 399
BbsI GAAGAC 1 cut(s) 448
Bbv12I GWGCWC 1 cut(s) 399
BbvI GCAGC 1 cut(s) 193
BccI CCATC 1 cut(s) 400
BceAI ACGGC 1 cut(s) 192
BcoDI GTCTC 1 cut(s) 444
BfaI CTAG 2 cut(s) 446, 513
BfmI CTRYAG 1 cut(s) 83
BfrI CTTAAG 1 cut(s) 188
BfuAI ACCTGC 1 cut(s) 466
BglII AGATCT 1 cut(s) 374
BisI GCNGC 1 cut(s) 207
BlsI GCNGC 1 cut(s) 208
Bme18I GGWCC 1 cut(s) 333
BmgT120I GGNCC 1 cut(s) 333
BmiI GGNNCC 1 cut(s) 415
BmsI GCATC 2 cut(s) 235, 316
BpiI GAAGAC 1 cut(s) 448
Bpu10I CCTNAGC 1 cut(s) 329
BsaHI GRCGYC 1 cut(s) 390
BsaI GGTCTC 1 cut(s) 444
BsaJI CCNNGG 1 cut(s) 70
Bsc4I CCNNNNNNNGG 1 cut(s) 290
Bse1I ACTGG 1 cut(s) 200
Bse21I CCTNAGG 1 cut(s) 256
BseDI CCNNGG 1 cut(s) 70
BseLI CCNNNNNNNGG 1 cut(s) 290
BseMII CTCAG 2 cut(s) 270, 343
BseNI ACTGG 1 cut(s) 200
BseRI GAGGAG 1 cut(s) 182
BseXI GCAGC 1 cut(s) 193
BsiHKAI GWGCWC 1 cut(s) 399
BslI CCNNNNNNNGG 1 cut(s) 290
BsmAI GTCTC 1 cut(s) 444
Bso31I GGTCTC 1 cut(s) 444
Bsp1286I GDGCHC 1 cut(s) 399
Bsp143I GATC 4 cut(s) 87, 140, 374, 485
BspACI CCGC 2 cut(s) 270, 417
BspCNI CTCAG 2 cut(s) 269, 342
BspLI GGNNCC 1 cut(s) 415
BspMI ACCTGC 1 cut(s) 466
BspPI GGATC 3 cut(s) 82, 148, 493
BspTI CTTAAG 1 cut(s) 188
BspTNI GGTCTC 1 cut(s) 444
BsrI ACTGG 1 cut(s) 200
BssECI CCNNGG 1 cut(s) 70
BssMI GATC 4 cut(s) 87, 140, 374, 485
BssNI GRCGYC 1 cut(s) 390
BssT1I CCWWGG 1 cut(s) 70
Bst4CI ACNGT 1 cut(s) 496
BstACI GRCGYC 1 cut(s) 390
BstAFI CTTAAG 1 cut(s) 188
BstDEI CTNAG 2 cut(s) 256, 329
BstKTI GATC 4 cut(s) 90, 143, 377, 488
BstMAI GTCTC 1 cut(s) 444
BstMBI GATC 4 cut(s) 87, 140, 374, 485
BstSFI CTRYAG 1 cut(s) 83
BstV1I GCAGC 1 cut(s) 193
BstV2I GAAGAC 1 cut(s) 448
BstX2I RGATCY 3 cut(s) 87, 140, 374
BstYI RGATCY 3 cut(s) 87, 140, 374
Bsu36I CCTNAGG 1 cut(s) 256
BveI ACCTGC 1 cut(s) 466
Cfr13I GGNCC 1 cut(s) 333
CseI GACGC 2 cut(s) 346, 398
Csp6I GTAC 1 cut(s) 294
CviAII CATG 2 cut(s) 27, 422
CviJI RGCY 6 cut(s) 80, 185, 206, 328, 397, 414
CviKI_1 RGCY 6 cut(s) 80, 185, 206, 328, 397, 414
CviQI GTAC 1 cut(s) 294
DdeI CTNAG 2 cut(s) 256, 329
DpnI GATC 4 cut(s) 89, 142, 376, 487
DpnII GATC 4 cut(s) 87, 140, 374, 485
Ecl136II GAGCTC 1 cut(s) 397
Eco130I CCWWGG 1 cut(s) 70
Eco24I GRGCYC 1 cut(s) 399
Eco31I GGTCTC 1 cut(s) 444
Eco47I GGWCC 1 cut(s) 333
Eco53kI GAGCTC 1 cut(s) 397
Eco81I CCTNAGG 1 cut(s) 256
EcoICRI GAGCTC 1 cut(s) 397
EcoT14I CCWWGG 1 cut(s) 70
EcoT38I GRGCYC 1 cut(s) 399
ErhI CCWWGG 1 cut(s) 70
FaeI CATG 2 cut(s) 30, 425
FaiI YATR 6 cut(s) 28, 94, 147, 149, 423, 492
FatI CATG 2 cut(s) 26, 421
FauI CCCGC 1 cut(s) 263
FauNDI CATATG 1 cut(s) 147
FblI GTMKAC 1 cut(s) 517
Fnu4HI GCNGC 1 cut(s) 207
FriOI GRGCYC 1 cut(s) 399
Fsp4HI GCNGC 1 cut(s) 207
FspBI CTAG 2 cut(s) 446, 513
GluI GCNGC 1 cut(s) 207
HgaI GACGC 2 cut(s) 346, 398
Hin1I GRCGYC 1 cut(s) 390
Hin1II CATG 2 cut(s) 30, 425
HincII GTYRAC 1 cut(s) 518
HindII GTYRAC 1 cut(s) 518
HphI GGTGA 1 cut(s) 291
Hpy166II GTNNAC 2 cut(s) 21, 518
Hpy188I TCNGA 5 cut(s) 10, 51, 346, 388, 403
Hpy188III TCNNGA 1 cut(s) 258
Hpy8I GTNNAC 2 cut(s) 21, 518
HpyAV CCTTC 3 cut(s) 118, 404, 476
HpyCH4III ACNGT 1 cut(s) 496
HpyCH4V TGCA 5 cut(s) 4, 96, 209, 307, 461
HpyF3I CTNAG 2 cut(s) 256, 329
Hsp92I GRCGYC 1 cut(s) 390
Hsp92II CATG 2 cut(s) 30, 425
Kzo9I GATC 4 cut(s) 87, 140, 374, 485
LmnI GCTCC 2 cut(s) 169, 419
LpnPI CCDG 4 cut(s) 181, 243, 316, 471
Lsp1109I GCAGC 1 cut(s) 193
LweI GCATC 2 cut(s) 235, 316
MaeI CTAG 2 cut(s) 446, 513
MaeIII GTNAC 1 cut(s) 98
MalI GATC 4 cut(s) 89, 142, 376, 487
MboI GATC 4 cut(s) 87, 140, 374, 485
MboII GAAGA 3 cut(s) 163, 243, 453
MflI RGATCY 3 cut(s) 87, 140, 374
MhlI GDGCHC 1 cut(s) 399
MluCI AATT 3 cut(s) 11, 159, 364
MmeI TCCRAC 1 cut(s) 369
MnlI CCTC 6 cut(s) 57, 123, 160, 222, 265, 371
MseI TTAA 1 cut(s) 189
MspCI CTTAAG 1 cut(s) 188
NdeI CATATG 1 cut(s) 147
NdeII GATC 4 cut(s) 87, 140, 374, 485
NlaIII CATG 2 cut(s) 30, 425
NlaIV GGNNCC 1 cut(s) 415
NmeAIII GCCGAG 1 cut(s) 418
PkrI GCNGC 1 cut(s) 208
Psp124BI GAGCTC 1 cut(s) 399
PspN4I GGNNCC 1 cut(s) 415
PspPI GGNCC 1 cut(s) 333
PsuI RGATCY 3 cut(s) 87, 140, 374
RsaI GTAC 1 cut(s) 295
RsaNI GTAC 1 cut(s) 294
SacI GAGCTC 1 cut(s) 399
SalI GTCGAC 1 cut(s) 516
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 1 cut(s) 207
Sau3AI GATC 4 cut(s) 87, 140, 374, 485
Sau96I GGNCC 1 cut(s) 333
SduI GDGCHC 1 cut(s) 399
SetI ASST 7 cut(s) 110, 330, 335, 351, 399, 460, 523
SfaNI GCATC 2 cut(s) 235, 316
SfcI CTRYAG 1 cut(s) 83
SinI GGWCC 1 cut(s) 333
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
Sse9I AATT 3 cut(s) 11, 159, 364
SsiI CCGC 2 cut(s) 270, 417
SspMI CTAG 2 cut(s) 446, 513
SstI GAGCTC 1 cut(s) 399
StyI CCWWGG 1 cut(s) 70
TaaI ACNGT 1 cut(s) 496
TaqI TCGA 1 cut(s) 517
TasI AATT 3 cut(s) 11, 159, 364
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TseI GCWGC 1 cut(s) 206
TspDTI ATGAA 2 cut(s) 164, 491
Vha464I CTTAAG 1 cut(s) 188
VpaK11BI GGWCC 1 cut(s) 333
XapI RAATTY 1 cut(s) 11
XmiI GTMKAC 1 cut(s) 517
XspI CTAG 2 cut(s) 446, 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.