MD06G1006100.v1.1

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0010571
Physical Location & Seq
Reverse (-)
637158 .. 642584
5427 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1006100.v1.1.491

Sequence Viewer

Length: 1152 bp
ATGGCAGCAGCAGCTGCGGGAGAAAGAAGTTTAAAACAGACGCCAACATGGGCTGTTGCCATCGCCTGTGCAGTTTTCATAGTTATTTCTATTATCATAGAACAAGCAATTCATGCTCTTGGAAAGTGGTTCCAGAAACGCAATAAGAAAGCTATGCTTGAAGCTTTGGAGAAAATAAAAGCTGAGCTAATGCTTTTGGGCTTCATATCTCTACTGCTTACTGTTGGAACACGATTTCTTCCAAAAATATGCATCTCAACCAAGTATGGTGACACCATGCTTCCATGTCAGAACGAGTCTAAGTACAGTGAGGGCGGCAAGGATGAAGGTAATAAAGGAGGAGGCGACGAGGATCGACGACGAAAGTTACTGTCACATGCTGAAAACATGATCTGGCGTCGAGTTTTAGCCTCTAGTGCTGGAGATTCATCTTCTTGCGCAGGAGGCAAAGTACCTTTGATATCTGCATCTGGGATGCACCAATTGCATGTATTCATCTTCGTGCTTGCTGTTTTTCATGTTTTCTACAGTGTCGTCACTATGGCACTTGCGCAGGCCAAAATGAAGAAATGGAAAGCTTGGGAATCAGAGACATCGTCCTTGGAATATCAGTTTACAAATGATCCTTCAAGATTCAGGTTTACTCGACAAACTTCGTTTGTGAAGCGTCACTCTGGCTTTTCTACAATGCCTGGAATAAGATGGATTGTGGCATTCTTCAGGCAATTCTTTGGTTCAGTAACAAGGGTGGACTATTTGACCATGCGCCACGGATTTATTAATGCACATTTTGCTCCAAATAGCAAATTTGACTTCCATAAGTATATTAAGAGATCCATGGAAGATGACTTTAAGGTCGTCGTGGGAATCAGTTTACCACTATGGATTTTTGCCGTCGTTTTTTTGCTTCTAAACGTTTACAAATGGTACACGCTCAATTGGTTGTCGTTGGCTCCTCTCATTATTCTTCTTCTGGTGGGTACAAAGCTTGAGCTTATCATCGTGGAGATGGCTCAAGAAATGCATGATCGGAATACAGTTGTTAAAGGCGCTCCCGTTGTGGAGCCAAACAACAAGTACTTTTGGTTTAACCGACCTGAGTGGACTCTTCTCTTAATTCATTTTACATTATTTCAGGTAAGCTTAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

384

Amino Acids

43.65

Weight (kDa)

9.68

Isoelectric Point (pI)

39.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 8 - 381 1.8e-175 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 854
Acc16I TGCGCA 2 cut(s) 439, 552
AciI CCGC 2 cut(s) 17, 315
AclI AACGTT 1 cut(s) 915
AclWI GGATC 3 cut(s) 360, 617, 828
AcsI RAATTY 1 cut(s) 806
AcuI CTGAAG 1 cut(s) 703
AcyI GRCGYC 2 cut(s) 41, 397
AfaI GTAC 5 cut(s) 305, 453, 929, 982, 1079
AgsI TTSAA 2 cut(s) 161, 630
AjnI CCWGG 1 cut(s) 691
AluBI AGCT 9 cut(s) 14, 152, 164, 182, 187, 578, 988, 994, 1143
AluI AGCT 9 cut(s) 14, 152, 164, 182, 187, 578, 988, 994, 1143
Alw26I GTCTC 1 cut(s) 584
AlwI GGATC 3 cut(s) 360, 617, 828
AlwNI CAGNNNCTG 1 cut(s) 14
AoxI GGCC 1 cut(s) 555
ApeKI GCWGC 4 cut(s) 5, 8, 11, 14
ApoI RAATTY 1 cut(s) 806
AseI ATTAAT 1 cut(s) 780
AspLEI GCGC 4 cut(s) 440, 553, 768, 1052
AsuHPI GGTGA 1 cut(s) 281
BbvI GCAGC 3 cut(s) 17, 20, 23
BccI CCATC 3 cut(s) 68, 696, 1003
BceAI ACGGC 1 cut(s) 878
BciT130I CCWGG 1 cut(s) 693
BcoDI GTCTC 1 cut(s) 584
BfaI CTAG 1 cut(s) 414
BfmI CTRYAG 1 cut(s) 526
BfoI RGCGCY 1 cut(s) 1053
BisI GCNGC 5 cut(s) 6, 9, 12, 15, 316
BlpI GCTNAGC 1 cut(s) 183
BlsI GCNGC 5 cut(s) 7, 10, 13, 16, 317
BmcAI AGTACT 1 cut(s) 1079
Bme1390I CCNGG 1 cut(s) 693
BmiI GGNNCC 3 cut(s) 131, 954, 1065
BmrFI CCNGG 1 cut(s) 693
BmsI GCATC 3 cut(s) 261, 465, 476
BpmI CTGGAG 1 cut(s) 441
Bpu1102I GCTNAGC 1 cut(s) 183
BpuEI CTTGAG 2 cut(s) 999, 1010
BsaHI GRCGYC 2 cut(s) 41, 397
BsaJI CCNNGG 3 cut(s) 600, 769, 837
BsaXI ACNNNNNCTCC 2 cut(s) 435, 465
BseBI CCWGG 1 cut(s) 693
BseDI CCNNGG 3 cut(s) 600, 769, 837
BseGI GGATG 2 cut(s) 328, 480
BseMII CTCAG 2 cut(s) 174, 1089
BseRI GAGGAG 2 cut(s) 354, 945
BseXI GCAGC 3 cut(s) 17, 20, 23
BsgI GTGCAG 1 cut(s) 90
BshFI GGCC 1 cut(s) 557
BsmAI GTCTC 1 cut(s) 584
BsmI GAATGC 1 cut(s) 713
BsnI GGCC 1 cut(s) 557
Bsp143I GATC 5 cut(s) 352, 390, 622, 833, 1027
Bsp1720I GCTNAGC 1 cut(s) 183
Bsp19I CCATGG 1 cut(s) 837
BspACI CCGC 2 cut(s) 17, 315
BspANI GGCC 1 cut(s) 557
BspCNI CTCAG 2 cut(s) 175, 1090
BspLI GGNNCC 3 cut(s) 131, 954, 1065
BspPI GGATC 3 cut(s) 360, 617, 828
BssECI CCNNGG 3 cut(s) 600, 769, 837
BssMI GATC 5 cut(s) 352, 390, 622, 833, 1027
BssNI GRCGYC 2 cut(s) 41, 397
BssT1I CCWWGG 2 cut(s) 600, 837
Bst2UI CCWGG 1 cut(s) 693
Bst4CI ACNGT 5 cut(s) 223, 308, 372, 530, 1039
Bst6I CTCTTC 1 cut(s) 1113
BstACI GRCGYC 2 cut(s) 41, 397
BstAPI GCANNNNNTGC 4 cut(s) 14, 113, 484, 791
BstC8I GCNNGC 2 cut(s) 507, 555
BstDEI CTNAG 3 cut(s) 183, 300, 1098
BstDSI CCRYGG 2 cut(s) 769, 837
BstF5I GGATG 2 cut(s) 328, 480
BstH2I RGCGCY 1 cut(s) 1053
BstHHI GCGC 4 cut(s) 440, 553, 768, 1052
BstKTI GATC 5 cut(s) 355, 393, 625, 836, 1030
BstMAI GTCTC 1 cut(s) 584
BstMBI GATC 5 cut(s) 352, 390, 622, 833, 1027
BstMWI GCNNNNNNNGC 7 cut(s) 11, 14, 113, 416, 444, 484, 791
BstNI CCWGG 1 cut(s) 693
BstNSI RCATGY 2 cut(s) 380, 491
BstSCI CCNGG 1 cut(s) 691
BstSFI CTRYAG 1 cut(s) 526
BstV1I GCAGC 3 cut(s) 17, 20, 23
BstX2I RGATCY 1 cut(s) 833
BstYI RGATCY 1 cut(s) 833
BsuRI GGCC 1 cut(s) 557
BtgI CCRYGG 2 cut(s) 769, 837
BtgZI GCGATG 1 cut(s) 46
BtsCI GGATG 2 cut(s) 328, 480
BtsIMutI CAGTG 2 cut(s) 313, 535
Cac8I GCNNGC 2 cut(s) 507, 555
CaiI CAGNNNCTG 1 cut(s) 14
CfoI GCGC 4 cut(s) 440, 553, 768, 1052
CseI GACGC 3 cut(s) 49, 386, 656
Csp6I GTAC 5 cut(s) 304, 452, 928, 981, 1078
CviQI GTAC 5 cut(s) 304, 452, 928, 981, 1078
DdeI CTNAG 3 cut(s) 183, 300, 1098
DpnI GATC 5 cut(s) 354, 392, 624, 835, 1029
DpnII GATC 5 cut(s) 352, 390, 622, 833, 1027
DraI TTTAAA 1 cut(s) 33
DrdI GACNNNNNNGTC 1 cut(s) 854
DseDI GACNNNNNNGTC 1 cut(s) 854
Eam1104I CTCTTC 1 cut(s) 1113
EarI CTCTTC 1 cut(s) 1113
Eco130I CCWWGG 2 cut(s) 600, 837
Eco32I GATATC 1 cut(s) 462
Eco57I CTGAAG 1 cut(s) 703
EcoRII CCWGG 1 cut(s) 691
EcoRV GATATC 1 cut(s) 462
EcoT14I CCWWGG 2 cut(s) 600, 837
EcoT22I ATGCAT 2 cut(s) 254, 1026
ErhI CCWWGG 2 cut(s) 600, 837
FalI AAGNNNNNCTT 2 cut(s) 141, 173
FauI CCCGC 1 cut(s) 10
Fnu4HI GCNGC 5 cut(s) 6, 9, 12, 15, 316
FokI GGATG 2 cut(s) 335, 487
Fsp4HI GCNGC 5 cut(s) 6, 9, 12, 15, 316
FspBI CTAG 1 cut(s) 414
FspI TGCGCA 2 cut(s) 439, 552
GlaI GCGC 4 cut(s) 439, 552, 767, 1051
GluI GCNGC 5 cut(s) 6, 9, 12, 15, 316
GsuI CTGGAG 1 cut(s) 441
HaeII RGCGCY 1 cut(s) 1053
HaeIII GGCC 1 cut(s) 557
HgaI GACGC 3 cut(s) 49, 386, 656
HhaI GCGC 4 cut(s) 440, 553, 768, 1052
Hin1I GRCGYC 2 cut(s) 41, 397
Hin6I GCGC 4 cut(s) 438, 551, 766, 1050
HinP1I GCGC 4 cut(s) 438, 551, 766, 1050
HindIII AAGCTT 4 cut(s) 162, 576, 986, 1141
HinfI GANTC 6 cut(s) 296, 425, 584, 633, 867, 1105
HphI GGTGA 1 cut(s) 281
Hpy166II GTNNAC 7 cut(s) 615, 642, 751, 875, 919, 930, 1104
Hpy188I TCNGA 3 cut(s) 291, 589, 1032
Hpy188III TCNNGA 3 cut(s) 133, 630, 1016
Hpy8I GTNNAC 7 cut(s) 615, 642, 751, 875, 919, 930, 1104
Hpy99I CGWCG 6 cut(s) 350, 360, 363, 402, 863, 899
HpyAV CCTTC 2 cut(s) 320, 636
HpyCH4III ACNGT 5 cut(s) 223, 308, 372, 530, 1039
HpyCH4IV ACGT 1 cut(s) 915
HpyCH4V TGCA 7 cut(s) 71, 252, 467, 478, 487, 785, 1024
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 14, 113, 416, 444, 484, 791
HpyF3I CTNAG 3 cut(s) 183, 300, 1098
HpySE526I ACGT 1 cut(s) 915
Hsp92I GRCGYC 2 cut(s) 41, 397
HspAI GCGC 4 cut(s) 438, 551, 766, 1050
Kzo9I GATC 5 cut(s) 352, 390, 622, 833, 1027
LmnI GCTCC 4 cut(s) 799, 958, 1057, 1063
Lsp1109I GCAGC 3 cut(s) 17, 20, 23
LweI GCATC 3 cut(s) 261, 465, 476
MaeI CTAG 1 cut(s) 414
MaeII ACGT 1 cut(s) 915
MaeIII GTNAC 6 cut(s) 269, 366, 372, 535, 668, 739
MalI GATC 5 cut(s) 354, 392, 624, 835, 1029
MboI GATC 5 cut(s) 352, 390, 622, 833, 1027
MboII GAAGA 9 cut(s) 230, 423, 490, 577, 709, 854, 959, 962, 1100
MfeI CAATTG 2 cut(s) 482, 937
MflI RGATCY 1 cut(s) 833
MluCI AATT 7 cut(s) 108, 482, 725, 806, 937, 1116, 1147
MlyI GAGTC 2 cut(s) 305, 1099
MmeI TCCRAC 1 cut(s) 205
MnlI CCTC 7 cut(s) 304, 332, 335, 343, 421, 437, 966
Mph1103I ATGCAT 2 cut(s) 254, 1026
MseI TTAA 9 cut(s) 32, 780, 828, 852, 1044, 1089, 1115, 1145, 1150
MslI CAYNNNNRTG 1 cut(s) 500
MspA1I CMGCKG 1 cut(s) 14
MspR9I CCNGG 1 cut(s) 693
MunI CAATTG 2 cut(s) 482, 937
Mva1269I GAATGC 1 cut(s) 713
MvaI CCWGG 1 cut(s) 693
MwoI GCNNNNNNNGC 7 cut(s) 11, 14, 113, 416, 444, 484, 791
NcoI CCATGG 1 cut(s) 837
NdeII GATC 5 cut(s) 352, 390, 622, 833, 1027
NlaIV GGNNCC 3 cut(s) 131, 954, 1065
NmuCI GTSAC 4 cut(s) 269, 372, 535, 668
NsbI TGCGCA 2 cut(s) 439, 552
NsiI ATGCAT 2 cut(s) 254, 1026
NspI RCATGY 2 cut(s) 380, 491
PctI GAATGC 1 cut(s) 713
PfeI GAWTC 4 cut(s) 425, 584, 633, 867
PflFI GACNNNGTC 1 cut(s) 595
PkrI GCNGC 5 cut(s) 7, 10, 13, 16, 317
PleI GAGTC 2 cut(s) 304, 1099
PpsI GAGTC 2 cut(s) 304, 1099
PshBI ATTAAT 1 cut(s) 780
Psp1406I AACGTT 1 cut(s) 915
Psp6I CCWGG 1 cut(s) 691
PspGI CCWGG 1 cut(s) 691
PspN4I GGNNCC 3 cut(s) 131, 954, 1065
PstNI CAGNNNCTG 1 cut(s) 14
PsuI RGATCY 1 cut(s) 833
PsyI GACNNNGTC 1 cut(s) 595
PvuII CAGCTG 1 cut(s) 14
RsaI GTAC 5 cut(s) 305, 453, 929, 982, 1079
RsaNI GTAC 5 cut(s) 304, 452, 928, 981, 1078
RseI CAYNNNNRTG 1 cut(s) 500
SaqAI TTAA 9 cut(s) 32, 780, 828, 852, 1044, 1089, 1115, 1145, 1150
SatI GCNGC 5 cut(s) 6, 9, 12, 15, 316
Sau3AI GATC 5 cut(s) 352, 390, 622, 833, 1027
ScaI AGTACT 1 cut(s) 1079
SchI GAGTC 2 cut(s) 305, 1099
ScrFI CCNGG 1 cut(s) 693
SfaNI GCATC 3 cut(s) 261, 465, 476
SfcI CTRYAG 1 cut(s) 526
SmiMI CAYNNNNRTG 1 cut(s) 500
SmlI CTYRAG 2 cut(s) 989, 1014
SmoI CTYRAG 2 cut(s) 989, 1014
Sse9I AATT 7 cut(s) 108, 482, 725, 806, 937, 1116, 1147
SsiI CCGC 2 cut(s) 17, 315
SspMI CTAG 1 cut(s) 414
StyD4I CCNGG 1 cut(s) 691
StyI CCWWGG 2 cut(s) 600, 837
TaaI ACNGT 5 cut(s) 223, 308, 372, 530, 1039
TaiI ACGT 1 cut(s) 918
TaqI TCGA 3 cut(s) 355, 400, 646
TasI AATT 7 cut(s) 108, 482, 725, 806, 937, 1116, 1147
TatI WGTACW 2 cut(s) 303, 1077
TauI GCSGC 1 cut(s) 318
TfiI GAWTC 4 cut(s) 425, 584, 633, 867
Tru1I TTAA 9 cut(s) 32, 780, 828, 852, 1044, 1089, 1115, 1145, 1150
Tru9I TTAA 9 cut(s) 32, 780, 828, 852, 1044, 1089, 1115, 1145, 1150
TscAI CASTG 2 cut(s) 313, 535
TseFI GTSAC 4 cut(s) 269, 372, 535, 668
TseI GCWGC 4 cut(s) 5, 8, 11, 14
Tsp45I GTSAC 4 cut(s) 269, 372, 535, 668
TspDTI ATGAA 9 cut(s) 67, 101, 193, 339, 417, 484, 506, 578, 1109
TspGWI ACGGA 1 cut(s) 786
TspRI CASTG 2 cut(s) 313, 535
Tth111I GACNNNGTC 1 cut(s) 595
VspI ATTAAT 1 cut(s) 780
XapI RAATTY 1 cut(s) 806
XceI RCATGY 2 cut(s) 380, 491
XspI CTAG 1 cut(s) 414
ZrmI AGTACT 1 cut(s) 1079
Zsp2I ATGCAT 2 cut(s) 254, 1026
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.