Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0007976

Physical Location & Seq

Genomic Coordinates Reverse (-)

716756 .. 721241

Gene Structure 4486 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1007000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2670 bp

ATGAGACGACCTCTCATCTTCCTTCTCCTATTTGCTCCGCTAATTGAATGTTTGACCAGAGAAGCAGCCATCACAGTCCCAGAACCAGAGACCGCGACTGCCCTCTCCATTCCACATTCCTCGCCTTCCAACCGCTTCAATTCTCTCCTGCCGAAGCTAGGAAATGATATAACGCTGGTTGTTGCATTGGATGGAACCATATATATGGTCAATACTAATCCATGGAGTATCCTGTGGGGCTTCCCTTCTGGGGCTCCAATTCATACTTCGTATCAGGATGCCAGTTTTTTGAACAATACTAGGTGTAATGATCACTTCATTGATTTGGGCGAAGACGGGGCGCTCTATAGCCATACCCGCAGCGGAAAAGAGAAATTGTCATCGAGTGTAGAAGAATTCATAGGAGGTGCTCCATATTGGTCAAAGGATGGAGGAGTCACAACGGGATCAAGGAGGAACACTGTGTTCCTTGTTAATGCCAAGACCGGAAAACTAATTCACATGTATAAATCGGCTGATACTCCGTCCAAGTTAGTGGTTTGGAGTACCGAAAGTGATCTGTCTCCGAAAGTGAAAGATGCTCACGGGTTGGTAGAGTCTGGTTCCATAGGCTTGGAAACCGTTGAACATATGCTTTACATTGTGAGAACAGATTATGAAATCACACATTATTCTTCTGGTAAGGTTGTTTGGACTGTGACATTTGCTACATTTGAATCTCGTCCCCAAATATCTAATACTGGAAATGAGCTTGCTTTAAGACACAGCGGGGTTTCCAACTCAGTATTGCCTAGTCAGAACAAAACTTATATCTCCCGTACTCTGGATCCCATATTGACAGAGTATCTTTCAAAATATGCTGGGATAAATGATAGCCATTCTGGAGACAGTTCTGCGTATGAGCATTCCCCTAATGCCAATGTTCCTGCGATTATTGAAGAGAATCATCCATCTCATGTTCCTGCGATTATTGAAGAGAATCATCCATCTCATGTTCCTGCGATTATTGAAGAGAATAATCCATCTGGTGGCAATGAAGGCATAATGGCTCAAGCTGTAACAAAATTACTGTCTCTGTGTCTCACACTATTGTCCATTGTAGTCTGTATCTGTCGCTGTCCAAGAGCATTTGGAAAACAACATAAACTCAAACAGGTGGCTGAGGAAACTAAGATGCAAGCTGGGGTTCCAAAGAAGAAGAGAACCCGTAGACTTGGAAACAATAAGAGAAATTCCATTGATGTCAAGAACATGAGCAATATGTCACATGAAGATAAGGTTGGAGAATCTAAATTTTTCATACAAAATGAAAGAAGTGAAATGAAGTTTCTCTTGACCTCAGCTGATCATCTTGATGGTCAAATAGAGGGGCGCAGGATTGGCAAGTTGCTTGTTTCCAACAATGAAATTGCTCAGGGAAGTAATGGTACCATTGTACTTGAGGGAATCTATGATGGTCGTGCGGTAGCTGTTAAACGTCTTGTGAGGACTCATCATGATGTGGCTTTCAAAGAGGTTCAGAATCTTATTGCTTCTGATCAGCATCCAAATATTGTACGTTGGCATGGAGTGGAGTATGATCAAGATTTTGTTTATCTATCCTTGGAGCGTTGTACATGTAGCTTATATGATCTTATTTATTTTCACTCTGGGTCCATTCAAAGCCAAATCACCACGGATCAGCATCCCCACTTTTGGACTGAGTATACCACTCGATTGCATTCCATGATGGGAAATAACAGGGACATTGAATTGTGGAAGGCAAATGGATACCCTTCACCCCAGTTGTTAAAATTAATGAGTGATCTGGTTTCTGGGCTTGCCCATTTACATGAACTTGGAATTATACATCGGGACTTAAAGCCTCAAAATGTTTTGATAATAAAGGGCAGGTCTTTATGTGCCAAGCTTTCAGATATGGGTATCAGCAAGCGCCTACAAGGGGACAAGTCTTCCATAACCCAGCATGCTACTGGTTATGGCAGTTCAGGCTGGCAAGCACCCGAACAACTTCTCCATCTGCGCCAAACGCGTGCAGTGGATTTGTTTAGTTTAGGCTGTCTACTCTTTTTCTGCGTCACTGGGGGCAAACACCCTTATGGTGATAGCATCGAGCGTGATGTAAATATAGTGAATGACCGCAAAGACTTGTTCTTAGTAGACACTATACCAGAAGCTGTAGACCTTTTTACTCGTCTCTTGAATCCCAACCCTGATCTTCGGCCAACATCCATGGATGTGTTGCATCATCCTTTCTTCTGGAGTTCGGAGACAAGACTTTCATTCCTTCGAGATGCAAGTGATCGGGTTGAATTGGAAGATAGGGAGAATGAGTCCGAACTACTAAATTCATTAGAAGGTACTGCAGCGGTGGCTTTAAATGGGAAGTGGGATGAAAAGATGGAACCTGCATTTATAAATAACATCGGACGATACAGGCGTTACAAGTTTGATAGCGTTCGTGATTTACTACGAGTGATACGGAACAAGTTAAATCACTACAGGGAACTTCCTGAGGAAGTGAAGGAGATATTGGGTCCAGTTCCTGAGGGGTTTGATGGATATTTTTCAAGTCGATTTCCAAAACTGTTAATCGAGGTTTATAAAGTGTTGTACAGGTGCTGCAAAGAGGAAGAGTTCTTCTGTAAGTATATAAAAAGCATCAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002376 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006983 GO:0006984 GO:0006986 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008380 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009816 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010467 GO:0010506 GO:0010508 GO:0012505 GO:0014070 GO:0016020 GO:0016070 GO:0019222 GO:0023052 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031984 GO:0033554 GO:0034263 GO:0034641 GO:0034976 GO:0035966 GO:0042175 GO:0042221 GO:0042493 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046483 GO:0046677 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071216 GO:0071704 GO:0090304 GO:0098542 GO:0098827 GO:1901360 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04140 ko04141 ko04210 ko04932 ko05010 map04140 map04141 map04210 map04932 map05010

Pfam Domains

Protein Families

Pkinase Ribonuc_2-5A

Protein Analysis

890

Amino Acids

100.02

Weight (kDa)

6.53

Isoelectric Point (pI)

46.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	467 - 753	1.5e-35	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	467 - 747	4.9e-26	Protein tyrosine and serine/threonine kinase
Ribonuc_2-5A	PF06479	759 - 884	1.2e-46	Ribonuclease 2-5A

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007976)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G24360 AT5G24360 AT5G24360
fragaria_vesca	FvH4_5g25750 FvH4_5g25750
malus_domestica	MD06G1007000.v1.1
prunus_persica	Prupe.5G008100_v2.0.a1 Prupe.5G008100_v2.0.a1 Prupe.5G008100_v2.0.a1
pyrus_communis	pycom02g08890 pycom06g00610
rosa_chinensis	RchiOBHm_Chr7g0216101
rosa_laevigata	RLG00000002594
rosa_multiflora	Rmu_co8312507.1_g000001 Rmu_sc0009767.1_g000002
rosa_roxburghii	Rroxscaffold_3G00243650
rosa_rugosa	Rorug07G0158900 Rorug07G0158900
rosa_samantha	Rh7AG295900 Rh7AG296000 Rh7BG288300 Rh7CG316300 Rh7DG298800
rosa_wichuraiana	Rw7G025170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2417, 2604
Acc36I	ACCTGC	2 cut(s)	1881, 2416
Acc65I	GGTACC	1 cut(s)	1427
AccB1I	GGYRCC	1 cut(s)	1427
AccB7I	CCANNNNNTGG	1 cut(s)	1028
AccI	GTMKAC	5 cut(s)	1210, 1706, 2062, 2160, 2181
AccII	CGCG	2 cut(s)	95, 2032
AciI	CCGC	9 cut(s)	38, 93, 133, 358, 363, 768, 1463, 2140, 2369
AclWI	GGATC	4 cut(s)	454, 821, 834, 1686
AcoI	YGGCCR	1 cut(s)	2222
AcsI	RAATTY	4 cut(s)	395, 1231, 1292, 2347
AfaI	GTAC	8 cut(s)	547, 820, 1429, 1437, 1557, 1615, 2362, 2615
AfiI	CCNNNNNNNGG	6 cut(s)	158, 250, 823, 1028, 1695, 1942
AflIII	ACRYGT	3 cut(s)	501, 1616, 2030
AjuI	GAANNNNNNNTTGG	6 cut(s)	250, 282, 1263, 1295, 2516, 2548
AloI	GAACNNNNNNTCC	2 cut(s)	1998, 2030
AluBI	AGCT	9 cut(s)	157, 751, 1055, 1181, 1343, 1469, 1623, 1909, 2177
AluI	AGCT	9 cut(s)	157, 751, 1055, 1181, 1343, 1469, 1623, 1909, 2177
Alw21I	GWGCWC	1 cut(s)	412
Alw26I	GTCTC	7 cut(s)	83, 567, 879, 1077, 1085, 2201, 2264
AlwI	GGATC	4 cut(s)	454, 821, 834, 1686
AlwNI	CAGNNNCTG	3 cut(s)	2177, 2546, 2622
AoxI	GGCC	1 cut(s)	2222
ApeKI	GCWGC	4 cut(s)	65, 360, 2366, 2622
ApoI	RAATTY	4 cut(s)	395, 1231, 1292, 2347
ArsI	GACNNNNNNTTYG	2 cut(s)	1055, 1087
AseI	ATTAAT	1 cut(s)	1796
Asp700I	GAANNNNTTC	1 cut(s)	2010
Asp718I	GGTACC	1 cut(s)	1427
AspLEI	GCGC	4 cut(s)	343, 1374, 1935, 2025
AspS9I	GGNCC	2 cut(s)	1653, 2537
AsuHPI	GGTGA	3 cut(s)	1663, 1770, 2114
AvaII	GGWCC	2 cut(s)	1653, 2537
AxyI	CCTNAGG	2 cut(s)	2514, 2547
BaeI	ACNNNNGTAYC	4 cut(s)	1419, 1452, 2425, 2458
BamHI	GGATCC	1 cut(s)	826
BanI	GGYRCC	1 cut(s)	1427
BanII	GRGCYC	1 cut(s)	256
BarI	GAAGNNNNNNTAC	2 cut(s)	1411, 1443
BbsI	GAAGAC	2 cut(s)	339, 1944
Bbv12I	GWGCWC	1 cut(s)	412
BbvCI	CCTCAGC	2 cut(s)	1161, 1339
BbvI	GCAGC	4 cut(s)	77, 372, 2378, 2609
BciVI	GTATCC	2 cut(s)	239, 1763
BclI	TGATCA	4 cut(s)	310, 1345, 1537, 1579
BcoDI	GTCTC	7 cut(s)	83, 567, 879, 1077, 1085, 2201, 2264
BfaI	CTAG	3 cut(s)	158, 300, 792
BfmI	CTRYAG	4 cut(s)	346, 2178, 2364, 2500
BfoI	RGCGCY	2 cut(s)	344, 1936
BfuAI	ACCTGC	2 cut(s)	1881, 2416
BfuI	GTATCC	2 cut(s)	239, 1763
BisI	GCNGC	4 cut(s)	66, 361, 2367, 2623
BlsI	GCNGC	4 cut(s)	67, 362, 2368, 2624
Bme18I	GGWCC	2 cut(s)	1653, 2537
BmgT120I	GGNCC	2 cut(s)	1653, 2537
BmiI	GGNNCC	9 cut(s)	196, 255, 604, 828, 1188, 1429, 1654, 2406, 2538
BmrI	ACTGGG	2 cut(s)	1777, 2091
BmsI	GCATC	8 cut(s)	268, 568, 1164, 1552, 1693, 2118, 2254, 2284
BmuI	ACTGGG	2 cut(s)	1777, 2091
BpiI	GAAGAC	2 cut(s)	339, 1944
BplI	GAGNNNNNCTC	1 cut(s)	27
BpmI	CTGGAG	2 cut(s)	903, 2281
Bpu10I	CCTNAGC	3 cut(s)	1161, 1339, 1413
BpuEI	CTTGAG	2 cut(s)	1035, 1460
BsaBI	GATNNNNATC	2 cut(s)	1542, 1683
BsaI	GGTCTC	1 cut(s)	83
BsaJI	CCNNGG	4 cut(s)	221, 1602, 1674, 2232
BsaWI	WCCGGW	1 cut(s)	485
BsaXI	ACNNNNNCTCC	4 cut(s)	1998, 2028, 2318, 2348
Bsc4I	CCNNNNNNNGG	6 cut(s)	158, 250, 823, 1028, 1695, 1942
Bse1I	ACTGG	6 cut(s)	282, 745, 1783, 1978, 2086, 2540
Bse21I	CCTNAGG	2 cut(s)	2514, 2547
Bse3DI	GCAATG	1 cut(s)	1039
Bse8I	GATNNNNATC	2 cut(s)	1542, 1683
BseDI	CCNNGG	4 cut(s)	221, 1602, 1674, 2232
BseJI	GATNNNNATC	2 cut(s)	1542, 1683
BseLI	CCNNNNNNNGG	6 cut(s)	158, 250, 823, 1028, 1695, 1942
BseMI	GCAATG	1 cut(s)	1039
BseMII	CTCAG	7 cut(s)	795, 1152, 1353, 1427, 1692, 2505, 2538
BseNI	ACTGG	6 cut(s)	282, 745, 1783, 1978, 2086, 2540
BseRI	GAGGAG	1 cut(s)	447
BseXI	GCAGC	4 cut(s)	77, 372, 2378, 2609
BseYI	CCCAGC	3 cut(s)	860, 1181, 1962
BsgI	GTGCAG	1 cut(s)	2055
Bsh1236I	CGCG	2 cut(s)	95, 2032
BshFI	GGCC	1 cut(s)	2224
BshNI	GGYRCC	1 cut(s)	1427
BsiHKAI	GWGCWC	1 cut(s)	412
BsiSI	CCGG	1 cut(s)	486
BslFI	GGGAC	5 cut(s)	62, 708, 1757, 1868, 1958
BslI	CCNNNNNNNGG	6 cut(s)	158, 250, 823, 1028, 1695, 1942
BsmAI	GTCTC	7 cut(s)	83, 567, 879, 1077, 1085, 2201, 2264
BsmBI	CGTCTC	1 cut(s)	2201
BsmFI	GGGAC	5 cut(s)	62, 708, 1757, 1868, 1958
BsmI	GAATGC	2 cut(s)	904, 1720
BsnI	GGCC	1 cut(s)	2224
Bso31I	GGTCTC	1 cut(s)	83
Bsp1286I	GDGCHC	2 cut(s)	256, 412
Bsp1407I	TGTACA	2 cut(s)	1613, 2613
Bsp19I	CCATGG	2 cut(s)	221, 2232
BspACI	CCGC	9 cut(s)	38, 93, 133, 358, 363, 768, 1463, 2140, 2369
BspANI	GGCC	1 cut(s)	2224
BspCNI	CTCAG	7 cut(s)	794, 1153, 1352, 1426, 1693, 2506, 2539
BspFNI	CGCG	2 cut(s)	95, 2032
BspHI	TCATGA	1 cut(s)	1495
BspLI	GGNNCC	9 cut(s)	196, 255, 604, 828, 1188, 1429, 1654, 2406, 2538
BspMAI	CTGCAG	1 cut(s)	2368
BspMI	ACCTGC	2 cut(s)	1881, 2416
BspPI	GGATC	4 cut(s)	454, 821, 834, 1686
BspT107I	GGYRCC	1 cut(s)	1427
BspTNI	GGTCTC	1 cut(s)	83
BsrDI	GCAATG	1 cut(s)	1039
BsrGI	TGTACA	2 cut(s)	1613, 2613
BsrI	ACTGG	6 cut(s)	282, 745, 1783, 1978, 2086, 2540
BssECI	CCNNGG	4 cut(s)	221, 1602, 1674, 2232
BssNAI	GTATAC	1 cut(s)	1707
BssT1I	CCWWGG	3 cut(s)	221, 1602, 2232
Bst1107I	GTATAC	1 cut(s)	1707
Bst4CI	ACNGT	7 cut(s)	76, 463, 622, 697, 890, 1071, 2589
Bst6I	CTCTTC	5 cut(s)	933, 969, 1005, 1193, 2628
BstAUI	TGTACA	2 cut(s)	1613, 2613
BstC8I	GCNNGC	8 cut(s)	753, 1179, 1821, 1931, 1968, 1994, 1998, 2034
BstDEI	CTNAG	9 cut(s)	781, 1161, 1170, 1339, 1413, 1701, 2155, 2514, 2547
BstDSI	CCRYGG	3 cut(s)	221, 1674, 2232
BstFNI	CGCG	2 cut(s)	95, 2032
BstH2I	RGCGCY	2 cut(s)	344, 1936
BstHHI	GCGC	4 cut(s)	343, 1374, 1935, 2025
BstMAI	GTCTC	7 cut(s)	83, 567, 879, 1077, 1085, 2201, 2264
BstMWI	GCNNNNNNNGC	6 cut(s)	357, 1038, 1380, 1989, 2029, 2372
BstNSI	RCATGY	3 cut(s)	505, 1620, 1970
BstSFI	CTRYAG	4 cut(s)	346, 2178, 2364, 2500
BstUI	CGCG	2 cut(s)	95, 2032
BstV1I	GCAGC	4 cut(s)	77, 372, 2378, 2609
BstV2I	GAAGAC	2 cut(s)	339, 1944
BstX2I	RGATCY	1 cut(s)	826
BstXI	CCANNNNNNTGG	3 cut(s)	205, 535, 613
BstYI	RGATCY	1 cut(s)	826
BstZ17I	GTATAC	1 cut(s)	1707
Bsu36I	CCTNAGG	2 cut(s)	2514, 2547
BsuI	GTATCC	2 cut(s)	239, 1763
BsuRI	GGCC	1 cut(s)	2224
BtgI	CCRYGG	3 cut(s)	221, 1674, 2232
BtsI	GCAGTG	1 cut(s)	2043
BtsIMutI	CAGTG	3 cut(s)	459, 2043, 2079
BveI	ACCTGC	2 cut(s)	1881, 2416
Cac8I	GCNNGC	8 cut(s)	753, 1179, 1821, 1931, 1968, 1994, 1998, 2034
CaiI	CAGNNNCTG	3 cut(s)	2177, 2546, 2622
CciI	TCATGA	1 cut(s)	1495
CfoI	GCGC	4 cut(s)	343, 1374, 1935, 2025
Cfr13I	GGNCC	2 cut(s)	1653, 2537
CseI	GACGC	1 cut(s)	2065
Csp6I	GTAC	8 cut(s)	546, 819, 1428, 1436, 1556, 1614, 2361, 2614
CviQI	GTAC	8 cut(s)	546, 819, 1428, 1436, 1556, 1614, 2361, 2614
DdeI	CTNAG	9 cut(s)	781, 1161, 1170, 1339, 1413, 1701, 2155, 2514, 2547
DraI	TTTAAA	1 cut(s)	2379
EaeI	YGGCCR	1 cut(s)	2222
Eam1104I	CTCTTC	5 cut(s)	933, 969, 1005, 1193, 2628
EarI	CTCTTC	5 cut(s)	933, 969, 1005, 1193, 2628
Eco130I	CCWWGG	3 cut(s)	221, 1602, 2232
Eco24I	GRGCYC	1 cut(s)	256
Eco31I	GGTCTC	1 cut(s)	83
Eco47I	GGWCC	2 cut(s)	1653, 2537
Eco81I	CCTNAGG	2 cut(s)	2514, 2547
EcoRI	GAATTC	1 cut(s)	395
EcoT14I	CCWWGG	3 cut(s)	221, 1602, 2232
EcoT38I	GRGCYC	1 cut(s)	256
ErhI	CCWWGG	3 cut(s)	221, 1602, 2232
Esp3I	CGTCTC	1 cut(s)	2201
FalI	AAGNNNNNCTT	2 cut(s)	1316, 1348
FaqI	GGGAC	5 cut(s)	62, 708, 1757, 1868, 1958
FauI	CCCGC	2 cut(s)	365, 761
FauNDI	CATATG	1 cut(s)	630
FbaI	TGATCA	4 cut(s)	310, 1345, 1537, 1579
FblI	GTMKAC	5 cut(s)	1210, 1706, 2062, 2160, 2181
Fnu4HI	GCNGC	4 cut(s)	66, 361, 2367, 2623
FriOI	GRGCYC	1 cut(s)	256
Fsp4HI	GCNGC	4 cut(s)	66, 361, 2367, 2623
FspBI	CTAG	3 cut(s)	158, 300, 792
GlaI	GCGC	4 cut(s)	342, 1373, 1934, 2024
GluI	GCNGC	4 cut(s)	66, 361, 2367, 2623
GsaI	CCCAGC	3 cut(s)	864, 1185, 1966
GsuI	CTGGAG	2 cut(s)	903, 2281
HaeII	RGCGCY	2 cut(s)	344, 1936
HaeIII	GGCC	1 cut(s)	2224
HapII	CCGG	1 cut(s)	486
HgaI	GACGC	1 cut(s)	2065
HhaI	GCGC	4 cut(s)	343, 1374, 1935, 2025
Hin6I	GCGC	4 cut(s)	341, 1372, 1933, 2023
HinP1I	GCGC	4 cut(s)	341, 1372, 1933, 2023
HindIII	AAGCTT	1 cut(s)	1907
HpaII	CCGG	1 cut(s)	486
HphI	GGTGA	3 cut(s)	1663, 1770, 2114
Hpy166II	GTNNAC	5 cut(s)	1211, 1707, 2063, 2161, 2182
Hpy188I	TCNGA	8 cut(s)	567, 798, 1521, 1537, 1915, 2269, 2338, 2429
Hpy8I	GTNNAC	5 cut(s)	1211, 1707, 2063, 2161, 2182
HpyAV	CCTTC	9 cut(s)	32, 135, 255, 1031, 1753, 1785, 2297, 2351, 2518
HpyCH4III	ACNGT	7 cut(s)	76, 463, 622, 697, 890, 1071, 2589
HpyCH4IV	ACGT	2 cut(s)	1477, 1558
HpyCH4V	TGCA	9 cut(s)	185, 1177, 1720, 2036, 2245, 2297, 2366, 2411, 2625
HpyF10VI	GCNNNNNNNGC	6 cut(s)	357, 1038, 1380, 1989, 2029, 2372
HpyF3I	CTNAG	9 cut(s)	781, 1161, 1170, 1339, 1413, 1701, 2155, 2514, 2547
HpySE526I	ACGT	2 cut(s)	1477, 1558
HspAI	GCGC	4 cut(s)	341, 1372, 1933, 2023
KpnI	GGTACC	1 cut(s)	1431
Ksp22I	TGATCA	4 cut(s)	310, 1345, 1537, 1579
LmnI	GCTCC	4 cut(s)	40, 259, 415, 1606
Lsp1109I	GCAGC	4 cut(s)	77, 372, 2378, 2609
LweI	GCATC	8 cut(s)	268, 568, 1164, 1552, 1693, 2118, 2254, 2284
MaeI	CTAG	3 cut(s)	158, 300, 792
MaeII	ACGT	2 cut(s)	1477, 1558
MaeIII	GTNAC	6 cut(s)	436, 697, 1057, 1263, 2077, 2441
MflI	RGATCY	1 cut(s)	826
MhlI	GDGCHC	2 cut(s)	256, 412
MluI	ACGCGT	1 cut(s)	2030
MlyI	GAGTC	4 cut(s)	444, 605, 1483, 2342
MmeI	TCCRAC	4 cut(s)	153, 801, 1261, 1422
MroXI	GAANNNNTTC	1 cut(s)	2010
MslI	CAYNNNNRTG	6 cut(s)	203, 1353, 1497, 1830, 2097, 2237
MspA1I	CMGCKG	4 cut(s)	363, 768, 1343, 2369
MspI	CCGG	1 cut(s)	486
Mva1269I	GAATGC	2 cut(s)	904, 1720
MvnI	CGCG	2 cut(s)	95, 2032
MwoI	GCNNNNNNNGC	6 cut(s)	357, 1038, 1380, 1989, 2029, 2372
NcoI	CCATGG	2 cut(s)	221, 2232
NdeI	CATATG	1 cut(s)	630
NlaIV	GGNNCC	9 cut(s)	196, 255, 604, 828, 1188, 1429, 1654, 2406, 2538
NmuCI	GTSAC	4 cut(s)	436, 697, 1263, 2077
NspI	RCATGY	3 cut(s)	505, 1620, 1970
PaeI	GCATGC	1 cut(s)	1970
PagI	TCATGA	1 cut(s)	1495
PciI	ACATGT	2 cut(s)	501, 1616
PcsI	WCGNNNNNNNCGW	1 cut(s)	2437
PctI	GAATGC	2 cut(s)	904, 1720
PdmI	GAANNNNTTC	1 cut(s)	2010
PfeI	GAWTC	7 cut(s)	716, 943, 979, 1286, 1446, 1522, 2203
PflMI	CCANNNNNTGG	1 cut(s)	1028
PkrI	GCNGC	4 cut(s)	67, 362, 2368, 2624
PleI	GAGTC	4 cut(s)	443, 604, 1483, 2341
PpsI	GAGTC	4 cut(s)	443, 604, 1483, 2341
PscI	ACATGT	2 cut(s)	501, 1616
PshBI	ATTAAT	1 cut(s)	1796
PsiI	TTATAA	2 cut(s)	2417, 2604
PspFI	CCCAGC	3 cut(s)	860, 1181, 1962
PspN4I	GGNNCC	9 cut(s)	196, 255, 604, 828, 1188, 1429, 1654, 2406, 2538
PspPI	GGNCC	2 cut(s)	1653, 2537
PstI	CTGCAG	1 cut(s)	2368
PstNI	CAGNNNCTG	3 cut(s)	2177, 2546, 2622
PsuI	RGATCY	1 cut(s)	826
PvuII	CAGCTG	1 cut(s)	1343
RsaI	GTAC	8 cut(s)	547, 820, 1429, 1437, 1557, 1615, 2362, 2615
RsaNI	GTAC	8 cut(s)	546, 819, 1428, 1436, 1556, 1614, 2361, 2614
RseI	CAYNNNNRTG	6 cut(s)	203, 1353, 1497, 1830, 2097, 2237
SatI	GCNGC	4 cut(s)	66, 361, 2367, 2623
Sau96I	GGNCC	2 cut(s)	1653, 2537
SchI	GAGTC	4 cut(s)	444, 605, 1483, 2342
SduI	GDGCHC	2 cut(s)	256, 412
SfaNI	GCATC	8 cut(s)	268, 568, 1164, 1552, 1693, 2118, 2254, 2284
SfcI	CTRYAG	4 cut(s)	346, 2178, 2364, 2500
SinI	GGWCC	2 cut(s)	1653, 2537
SmiMI	CAYNNNNRTG	6 cut(s)	203, 1353, 1497, 1830, 2097, 2237
SmlI	CTYRAG	2 cut(s)	1050, 1439
SmoI	CTYRAG	2 cut(s)	1050, 1439
SphI	GCATGC	1 cut(s)	1970
SsiI	CCGC	9 cut(s)	38, 93, 133, 358, 363, 768, 1463, 2140, 2369
SspI	AATATT	1 cut(s)	1552
SspMI	CTAG	3 cut(s)	158, 300, 792
StyI	CCWWGG	3 cut(s)	221, 1602, 2232
TaaI	ACNGT	7 cut(s)	76, 463, 622, 697, 890, 1071, 2589
TaiI	ACGT	2 cut(s)	1480, 1561
TaqI	TCGA	6 cut(s)	383, 1714, 2112, 2290, 2575, 2595
TatI	WGTACW	3 cut(s)	1435, 1613, 2613
TfiI	GAWTC	7 cut(s)	716, 943, 979, 1286, 1446, 1522, 2203
TscAI	CASTG	3 cut(s)	466, 2043, 2086
TseFI	GTSAC	4 cut(s)	436, 697, 1263, 2077
TseI	GCWGC	4 cut(s)	65, 360, 2366, 2622
Tsp45I	GTSAC	4 cut(s)	436, 697, 1263, 2077
TspGWI	ACGGA	3 cut(s)	513, 1691, 2497
TspRI	CASTG	3 cut(s)	466, 2043, 2086
Van91I	CCANNNNNTGG	1 cut(s)	1028
VpaK11BI	GGWCC	2 cut(s)	1653, 2537
VspI	ATTAAT	1 cut(s)	1796
XapI	RAATTY	4 cut(s)	395, 1231, 1292, 2347
XceI	RCATGY	3 cut(s)	505, 1620, 1970
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1970
XmiI	GTMKAC	5 cut(s)	1210, 1706, 2062, 2160, 2181
XmnI	GAANNNNTTC	1 cut(s)	2010
XspI	CTAG	3 cut(s)	158, 300, 792

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.