Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0007976

Physical Location & Seq

Genomic Coordinates Forward (+)

36748275 .. 36752404

Gene Structure 4130 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000002594

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2733 bp

ATGAGACGATCGGTGATCTTCCTTCTCCTATTTGCTCCCTTGATCGGCTGTTTCGTCAAGTCTGGTTCAGCCGAGGAGACCTCCCTCGCCAATCCAGATTCCGCCGGCGGCGCTCTTCAGCTCTTCAGCTCGCTTCTGCCTCCTGCACTTAGGAATGATGGAGCGCTTTTGGTCACATTGGATGGCAAGGTAGTTTCTGTCAACCCCAAGACGATGGAGATTGAGTGGGCCTTGTCCTCTGGTTCCCGAATTCACTCTTCCTATCAGAACGTCTCTTATTTCAACAACAGCAGGAGTGATGATGATGATGATGATCACTCTTTCGTTGATATTGGTGAAGACTGGGCCCTCTATCGTCACAGCAACACCAACCAGAAGAAGAAGAAACTGTCGTCGAGTGTTAGAGAATATGTTGCAAGTACTCCGCTAGTTTCTGAGGATGGGGGAGTCACATTGGGGTCAAAGATCACTAAAGTATTTGCCGTTAATGCTGTGACGGGACAAGTAATTCATAGTTTTAGGTTGGGTGATAATACTCCTTCAGCTACTTTAGGTATGGAACCCACCGGTGTCGAGGGGTTGGAAACATCTGGACTGGTGTTGTATCTCGAGAGAACAGATTACACACTGCAGCATTGTACAGTCAAGGGCGATCTTCTGTGGAAATTAGAAATTGCAGAGTTTGATGCTCATTTTCGATCTCCTAAAATTGGAAATGGGCTGGGTCTAGAGGACAGGCTCATTACTAACTCTACAGTGCCTTATCAGAAGGAGAAGCCTGTTGTCATTCGGGTTCCTTATCCTAGCTCGGTGGAATCTCGTTCTGTAATTGACGGTCTAACTGATGGGTATTATGGAGGCAAACCTCTCCCCCTTGAAGGTCCTCATAACGATATTCTGGCACTGCCTTTTGACAAAGGTGCTGTACCTCCTCACAACAATGAAGGCAGAGAAATGCTTGCTTTGCCCTCTCTTGAAGTTGTGGACTCTAGGATCTTTGCTCTCCCTAGTAGAGATGTTGGTAAACTGGACATCACAAGCTCAGTGGCTGAATCTATTCCTAACTTCCCCATACAATCTCTTTTTGTATTTCTTCTCACCCTATTATCCATTCTGGGATATATCTTTCGCTTTCTGACCGGAAAACAGCGTAAACTGAAAGTGGTGGCTGAGGAAACTCTGAAAGTGGTGGCTGAGGAAACTCTGAAAGTGGTGGCTGAGGAAACTAAAGCGCAAGCAGGAGCTCCCAAAAAGAAGAAAACTCGAAGATTGGGAAATAATAAGAAGAATATCAGTTATGAGAAAGACGCAAGAAATATGTCGTATGAATATAAGTTTGGTGAATCTAATGGACCTGCACATGGGGAAAGAAATGAAACAAAATTGCTCTTGACCTCTTCGGATTTTATTGATCGTGAAACCGAGGGACGTAGGATTGGCAAGTTACTTGTTTCCAGTAATGAAATTGCTAAGGGAAGTAACGGTACCATTGTACTTGAGGGTATCTATGATGGTCGTCCAGTAGCTGTTAAACGCCTTGTGCGAACTCATCATGACGTGGCTTTGAAAGAGATTCAGAATCTTATTGCGTCTGATCAGCATCCAAATATTGTCCGTTGGTATGGCGTGGAGCATGATCAAGATTTTGTCTATCTATCCTTGGAGCGCTGCTCATGTAGCTTAAATGACTTGATTTATTATTACTCAGAGTCTTCTCAAGGGCAAATTATCAACAAGGATGAGGATCCTCACTTTTTGGCCGAGTATCGCATTCAATTGCAGGCAATCATGGGAAAGAACAAGAATGTTGAATTGTGGAAGACAAATGGATACCCTTCACCCCAAATGTTAAAATTAATGAGTGATCTTGTTTCTGGACTTGCCCATTTGCATGAACTTGGAATTATACATCGGGATCTAAAGCCTCAAAATGTTTTGATAATCAAGGGCAGATCGTTATGTGCCAAGCTTTCAGATATGGGTATCAGCAAACGCCTTCAAGGGGACAGGTCTTCCATAACCCAGCATGCTACTGGTTATGGGAGTTCAGGTTGGCAAGCACCTGAACAACTTCTCCATCAGCGCCAAACACGTGCAGTAGATTTATTTAGTTTAGGTTGTCTCCTCTTTTTCTGTCTCACTGGGGGTAAACACCCTTATGGGGATAGTATCGAGCGTGATGTGAATATAGTGAATGATCGGAAAGACCTATTCTTGGTAGAGAATATACCAGAAGCAATGGATCTTTTTACTCGTCTCTTGGATCCCAACCCTGACATGAGGCCAACAGCCGTGGATGTGTTGCATCATCCCTTTTTCTGGAGTTCTGAGACGAGACTTTCATTTCTGCGTGATGCTAGTGATCGGGTTGAATTGGAGGACAGGGAGACTGCATCCCAACTCTTAAGTTCATTAGAAAGTACTGCAGCAGTTGCTCTAAATGGTAAGTGGGATGAAAAGATGGAACCTGCATTCCTCAATAACATTGGTCGCTACAGGCGTTACAAGTTTGACAGCATCCGTGACTTGCTGCGTGTAATAAGGAACAAGTTAAATCACTACAGAGAACTTCCCCAAGAGATCCAGGAATTATTGGGGACAGTTCCTGAGGGCTTCGACAGTTATTTTTCAAGTCGATTCCCTAAATTATTAATCGAGGTTTATAATGTGTTGTACAAGTACTGCAAAGAGGAGGAATTTTTTCGCAAGTATATACAAGGCAAATCGGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002376 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006983 GO:0006984 GO:0006986 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008380 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009816 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010467 GO:0010506 GO:0010508 GO:0012505 GO:0014070 GO:0016020 GO:0016070 GO:0019222 GO:0023052 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031984 GO:0033554 GO:0034263 GO:0034641 GO:0034976 GO:0035966 GO:0042175 GO:0042221 GO:0042493 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046483 GO:0046677 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071216 GO:0071704 GO:0090304 GO:0098542 GO:0098827 GO:1901360 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04140 ko04141 ko04210 ko04932 ko05010 map04140 map04141 map04210 map04932 map05010

Pfam Domains

Protein Families

PQQ Pkinase Ribonuc_2-5A

Protein Analysis

911

Amino Acids

102.16

Weight (kDa)

6.33

Isoelectric Point (pI)

41.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	486 - 767	5.2e-27	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	487 - 773	3e-37	Protein kinase domain
Ribonuc_2-5A	PF06479	779 - 904	1.4e-46	Ribonuclease 2-5A

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007976)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G24360 AT5G24360 AT5G24360
fragaria_vesca	FvH4_5g25750 FvH4_5g25750
malus_domestica	MD06G1007000.v1.1
prunus_persica	Prupe.5G008100_v2.0.a1 Prupe.5G008100_v2.0.a1 Prupe.5G008100_v2.0.a1
pyrus_communis	pycom02g08890 pycom06g00610
rosa_chinensis	RchiOBHm_Chr7g0216101
rosa_laevigata	RLG00000002594
rosa_multiflora	Rmu_co8312507.1_g000001 Rmu_sc0009767.1_g000002
rosa_roxburghii	Rroxscaffold_3G00243650
rosa_rugosa	Rorug07G0158900 Rorug07G0158900
rosa_samantha	Rh7AG295900 Rh7AG296000 Rh7BG288300 Rh7CG316300 Rh7DG298800
rosa_wichuraiana	Rw7G025170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2664
Acc36I	ACCTGC	2 cut(s)	1363, 2476
Acc65I	GGTACC	1 cut(s)	1484
AccB1I	GGYRCC	1 cut(s)	1484
AciI	CCGC	3 cut(s)	102, 108, 425
AclWI	GGATC	8 cut(s)	1001, 1739, 1752, 1923, 2250, 2258, 2271, 2575
AcoI	YGGCCR	1 cut(s)	1758
AcsI	RAATTY	2 cut(s)	249, 2696
AcuI	CTGAAG	3 cut(s)	101, 109, 525
AcvI	CACGTG	1 cut(s)	2093
AfaI	GTAC	8 cut(s)	421, 640, 927, 1486, 1494, 2422, 2675, 2681
AfeI	AGCGCT	2 cut(s)	165, 1667
AfiI	CCNNNNNNNGG	8 cut(s)	44, 710, 878, 1361, 2002, 2161, 2215, 2319
AflII	CTTAAG	1 cut(s)	2404
AflIII	ACRYGT	1 cut(s)	2090
AgeI	ACCGGT	1 cut(s)	566
AgsI	TTSAA	9 cut(s)	283, 878, 977, 1567, 1775, 1811, 2000, 2372, 2631
AjiI	CACGTC	1 cut(s)	1558
AjnI	CCWGG	1 cut(s)	2583
AjuI	GAANNNNNNNTTGG	2 cut(s)	1320, 1352
AloI	GAACNNNNNNTCC	4 cut(s)	2058, 2090, 2457, 2489
AluBI	AGCT	9 cut(s)	121, 129, 545, 807, 1041, 1244, 1526, 1680, 1969
AluI	AGCT	9 cut(s)	121, 129, 545, 807, 1041, 1244, 1526, 1680, 1969
Alw21I	GWGCWC	1 cut(s)	1246
Alw26I	GTCTC	8 cut(s)	71, 277, 2126, 2141, 2261, 2324, 2329, 2381
AlwI	GGATC	8 cut(s)	1001, 1739, 1752, 1923, 2250, 2258, 2271, 2575
AlwNI	CAGNNNCTG	3 cut(s)	1049, 1526, 2606
Ama87I	CYCGRG	1 cut(s)	608
Aor51HI	AGCGCT	2 cut(s)	165, 1667
AoxI	GGCC	4 cut(s)	228, 345, 1758, 2282
ApaI	GGGCCC	1 cut(s)	349
ApeKI	GCWGC	4 cut(s)	631, 1668, 2426, 2530
ApoI	RAATTY	2 cut(s)	249, 2696
AseI	ATTAAT	2 cut(s)	1856, 2651
AsiGI	ACCGGT	1 cut(s)	566
Asp700I	GAANNNNTTC	3 cut(s)	1056, 2070, 2700
Asp718I	GGTACC	1 cut(s)	1484
AspLEI	GCGC	5 cut(s)	113, 166, 1234, 1668, 2085
AspS9I	GGNCC	5 cut(s)	228, 345, 346, 881, 1352
AsuHPI	GGTGA	6 cut(s)	25, 347, 539, 1090, 1352, 1830
AvaI	CYCGRG	1 cut(s)	608
AvaII	GGWCC	2 cut(s)	881, 1352
AxyI	CCTNAGG	1 cut(s)	2607
BaeGI	GKGCMC	1 cut(s)	349
BaeI	ACNNNNGTAYC	2 cut(s)	1476, 1509
BamHI	GGATCC	2 cut(s)	1744, 2263
BanI	GGYRCC	1 cut(s)	1484
BanII	GRGCYC	2 cut(s)	349, 1246
BarI	GAAGNNNNNNTAC	2 cut(s)	1468, 1500
BbrPI	CACGTG	1 cut(s)	2093
BbsI	GAAGAC	4 cut(s)	345, 1704, 1826, 2004
Bbv12I	GWGCWC	1 cut(s)	1246
BbvCI	CCTCAGC	3 cut(s)	1170, 1194, 1218
BbvI	GCAGC	4 cut(s)	643, 1655, 2438, 2517
BccI	CCATC	8 cut(s)	152, 176, 208, 434, 839, 1505, 2085, 2455
BceAI	ACGGC	2 cut(s)	467, 2276
BciT130I	CCWGG	1 cut(s)	2585
BciVI	GTATCC	1 cut(s)	1823
BclI	TGATCA	3 cut(s)	313, 1594, 1636
BcoDI	GTCTC	8 cut(s)	71, 277, 2126, 2141, 2261, 2324, 2329, 2381
BfaI	CTAG	6 cut(s)	428, 728, 804, 990, 1008, 2358
BfmI	CTRYAG	5 cut(s)	629, 753, 2424, 2494, 2560
BfoI	RGCGCY	4 cut(s)	114, 167, 1669, 2086
BfrI	CTTAAG	1 cut(s)	2404
BfuAI	ACCTGC	2 cut(s)	1363, 2476
BfuI	GTATCC	1 cut(s)	1823
BisI	GCNGC	5 cut(s)	109, 632, 1669, 2427, 2531
BlsI	GCNGC	5 cut(s)	110, 633, 1670, 2428, 2532
BmcAI	AGTACT	3 cut(s)	421, 2422, 2681
Bme1390I	CCNGG	1 cut(s)	2585
Bme18I	GGWCC	2 cut(s)	881, 1352
BmeT110I	CYCGRG	1 cut(s)	608
BmgBI	CACGTC	1 cut(s)	1558
BmgT120I	GGNCC	5 cut(s)	228, 345, 346, 881, 1352
BmiI	GGNNCC	8 cut(s)	244, 347, 561, 795, 1486, 1746, 2265, 2466
BmrFI	CCNGG	1 cut(s)	2585
BmrI	ACTGGG	2 cut(s)	352, 2151
BmsI	GCATC	6 cut(s)	676, 1609, 2314, 2344, 2402, 2526
BmuI	ACTGGG	2 cut(s)	352, 2151
BpiI	GAAGAC	4 cut(s)	345, 1704, 1826, 2004
BplI	GAGNNNNNCTC	4 cut(s)	65, 97, 1655, 1687
BpmI	CTGGAG	1 cut(s)	2341
Bpu10I	CCTNAGC	4 cut(s)	1170, 1194, 1218, 1470
BpuEI	CTTGAG	2 cut(s)	1517, 1701
BsaAI	YACGTR	1 cut(s)	2093
BsaBI	GATNNNNATC	3 cut(s)	312, 1599, 1743
BsaI	GGTCTC	1 cut(s)	71
BsaJI	CCNNGG	4 cut(s)	72, 1422, 1659, 2292
BsaWI	WCCGGW	2 cut(s)	566, 1139
BsaXI	ACNNNNNCTCC	6 cut(s)	209, 239, 2035, 2058, 2065, 2088
Bsc4I	CCNNNNNNNGG	8 cut(s)	44, 710, 878, 1361, 2002, 2161, 2215, 2319
Bse118I	RCCGGY	2 cut(s)	104, 566
Bse1I	ACTGG	7 cut(s)	347, 600, 1032, 1455, 1520, 2038, 2146
Bse21I	CCTNAGG	1 cut(s)	2607
Bse3DI	GCAATG	1 cut(s)	2244
Bse8I	GATNNNNATC	3 cut(s)	312, 1599, 1743
BseBI	CCWGG	1 cut(s)	2585
BseDI	CCNNGG	4 cut(s)	72, 1422, 1659, 2292
BseGI	GGATG	9 cut(s)	187, 445, 1600, 1744, 2302, 2308, 2393, 2458, 2517
BseJI	GATNNNNATC	3 cut(s)	312, 1599, 1743
BseLI	CCNNNNNNNGG	8 cut(s)	44, 710, 878, 1361, 2002, 2161, 2215, 2319
BseMI	GCAATG	1 cut(s)	2244
BseMII	CTCAG	8 cut(s)	426, 1056, 1161, 1185, 1209, 1719, 2319, 2598
BseNI	ACTGG	7 cut(s)	347, 600, 1032, 1455, 1520, 2038, 2146
BseRI	GAGGAG	4 cut(s)	89, 921, 2114, 2705
BseSI	GKGCMC	1 cut(s)	349
BseXI	GCAGC	4 cut(s)	643, 1655, 2438, 2517
BseYI	CCCAGC	2 cut(s)	721, 2022
BsgI	GTGCAG	3 cut(s)	129, 1341, 2115
Bsh1285I	CGRYCG	1 cut(s)	11
BshFI	GGCC	4 cut(s)	230, 347, 1760, 2284
BshNI	GGYRCC	1 cut(s)	1484
BshTI	ACCGGT	1 cut(s)	566
BsiEI	CGRYCG	1 cut(s)	11
BsiHKAI	GWGCWC	1 cut(s)	1246
BsiHKCI	CYCGRG	1 cut(s)	608
BsiSI	CCGG	3 cut(s)	105, 567, 1140
BslFI	GGGAC	4 cut(s)	513, 1440, 2018, 2611
BslI	CCNNNNNNNGG	8 cut(s)	44, 710, 878, 1361, 2002, 2161, 2215, 2319
BsmAI	GTCTC	8 cut(s)	71, 277, 2126, 2141, 2261, 2324, 2329, 2381
BsmBI	CGTCTC	3 cut(s)	277, 2261, 2324
BsmFI	GGGAC	4 cut(s)	513, 1440, 2018, 2611
BsmI	GAATGC	2 cut(s)	1770, 2471
BsnI	GGCC	4 cut(s)	230, 347, 1760, 2284
Bso31I	GGTCTC	1 cut(s)	71
BsoBI	CYCGRG	1 cut(s)	608
Bsp120I	GGGCCC	1 cut(s)	345
Bsp1286I	GDGCHC	2 cut(s)	349, 1246
Bsp1407I	TGTACA	2 cut(s)	638, 2673
BspACI	CCGC	3 cut(s)	102, 108, 425
BspANI	GGCC	4 cut(s)	230, 347, 1760, 2284
BspCNI	CTCAG	8 cut(s)	427, 1055, 1162, 1186, 1210, 1718, 2320, 2599
BspHI	TCATGA	1 cut(s)	1552
BspLI	GGNNCC	8 cut(s)	244, 347, 561, 795, 1486, 1746, 2265, 2466
BspMAI	CTGCAG	2 cut(s)	633, 2428
BspMI	ACCTGC	2 cut(s)	1363, 2476
BspPI	GGATC	8 cut(s)	1001, 1739, 1752, 1923, 2250, 2258, 2271, 2575
BspQI	GCTCTTC	2 cut(s)	120, 128
BspT107I	GGYRCC	1 cut(s)	1484
BspTI	CTTAAG	1 cut(s)	2404
BspTNI	GGTCTC	1 cut(s)	71
BsrDI	GCAATG	1 cut(s)	2244
BsrFI	RCCGGY	2 cut(s)	104, 566
BsrGI	TGTACA	2 cut(s)	638, 2673
BsrI	ACTGG	7 cut(s)	347, 600, 1032, 1455, 1520, 2038, 2146
BssAI	RCCGGY	2 cut(s)	104, 566
BssECI	CCNNGG	4 cut(s)	72, 1422, 1659, 2292
BssT1I	CCWWGG	1 cut(s)	1659
Bst2UI	CCWGG	1 cut(s)	2585
Bst4CI	ACNGT	7 cut(s)	390, 643, 757, 836, 1484, 2602, 2621
Bst6I	CTCTTC	4 cut(s)	120, 128, 262, 1402
BstAFI	CTTAAG	1 cut(s)	2404
BstAPI	GCANNNNNTGC	1 cut(s)	2432
BstAUI	TGTACA	2 cut(s)	638, 2673
BstBAI	YACGTR	1 cut(s)	2093
BstC8I	GCNNGC	7 cut(s)	106, 131, 960, 1236, 1782, 2028, 2058
BstDSI	CCRYGG	1 cut(s)	2292
BstF5I	GGATG	9 cut(s)	187, 445, 1600, 1744, 2302, 2308, 2393, 2458, 2517
BstH2I	RGCGCY	4 cut(s)	114, 167, 1669, 2086
BstHHI	GCGC	5 cut(s)	113, 166, 1234, 1668, 2085
BstMAI	GTCTC	8 cut(s)	71, 277, 2126, 2141, 2261, 2324, 2329, 2381
BstMCI	CGRYCG	1 cut(s)	11
BstMWI	GCNNNNNNNGC	5 cut(s)	110, 488, 964, 1677, 2432
BstNI	CCWGG	1 cut(s)	2585
BstNSI	RCATGY	1 cut(s)	2030
BstSCI	CCNGG	1 cut(s)	2583
BstSFI	CTRYAG	5 cut(s)	629, 753, 2424, 2494, 2560
BstSLI	GKGCMC	1 cut(s)	349
BstV1I	GCAGC	4 cut(s)	643, 1655, 2438, 2517
BstV2I	GAAGAC	4 cut(s)	345, 1704, 1826, 2004
BstX2I	RGATCY	6 cut(s)	993, 1744, 1915, 2242, 2263, 2580
BstXI	CCANNNNNNTGG	1 cut(s)	214
BstYI	RGATCY	6 cut(s)	993, 1744, 1915, 2242, 2263, 2580
Bsu36I	CCTNAGG	1 cut(s)	2607
BsuI	GTATCC	1 cut(s)	1823
BsuRI	GGCC	4 cut(s)	230, 347, 1760, 2284
BtgI	CCRYGG	1 cut(s)	2292
BtrI	CACGTC	1 cut(s)	1558
BtsCI	GGATG	9 cut(s)	187, 445, 1600, 1744, 2302, 2308, 2393, 2458, 2517
BtsI	GCAGTG	2 cut(s)	626, 902
BtsIMutI	CAGTG	5 cut(s)	626, 762, 902, 1050, 2139
BveI	ACCTGC	2 cut(s)	1363, 2476
Cac8I	GCNNGC	7 cut(s)	106, 131, 960, 1236, 1782, 2028, 2058
CaiI	CAGNNNCTG	3 cut(s)	1049, 1526, 2606
CciI	TCATGA	1 cut(s)	1552
CfoI	GCGC	5 cut(s)	113, 166, 1234, 1668, 2085
Cfr10I	RCCGGY	2 cut(s)	104, 566
Cfr13I	GGNCC	5 cut(s)	228, 345, 346, 881, 1352
CseI	GACGC	2 cut(s)	1316, 1578
Csp6I	GTAC	8 cut(s)	420, 639, 926, 1485, 1493, 2421, 2674, 2680
CspAI	ACCGGT	1 cut(s)	566
CspCI	CAANNNNNGTGG	4 cut(s)	1026, 1061, 2274, 2309
CviAII	CATG	8 cut(s)	1361, 1553, 1634, 1674, 1789, 1892, 2027, 2278
CviQI	GTAC	8 cut(s)	420, 639, 926, 1485, 1493, 2421, 2674, 2680
EaeI	YGGCCR	1 cut(s)	1758
Eam1104I	CTCTTC	4 cut(s)	120, 128, 262, 1402
EarI	CTCTTC	4 cut(s)	120, 128, 262, 1402
EciI	GGCGGA	1 cut(s)	91
Ecl136II	GAGCTC	1 cut(s)	1244
Eco130I	CCWWGG	1 cut(s)	1659
Eco24I	GRGCYC	2 cut(s)	349, 1246
Eco31I	GGTCTC	1 cut(s)	71
Eco47I	GGWCC	2 cut(s)	881, 1352
Eco47III	AGCGCT	2 cut(s)	165, 1667
Eco53kI	GAGCTC	1 cut(s)	1244
Eco57I	CTGAAG	3 cut(s)	101, 109, 525
Eco72I	CACGTG	1 cut(s)	2093
Eco81I	CCTNAGG	1 cut(s)	2607
Eco88I	CYCGRG	1 cut(s)	608
EcoICRI	GAGCTC	1 cut(s)	1244
EcoO109I	RGGNCCY	2 cut(s)	346, 881
EcoRI	GAATTC	1 cut(s)	249
EcoRII	CCWGG	1 cut(s)	2583
EcoT14I	CCWWGG	1 cut(s)	1659
EcoT38I	GRGCYC	2 cut(s)	349, 1246
ErhI	CCWWGG	1 cut(s)	1659
Esp3I	CGTCTC	3 cut(s)	277, 2261, 2324
FaeI	CATG	8 cut(s)	1364, 1556, 1637, 1677, 1792, 1895, 2030, 2281
FaqI	GGGAC	4 cut(s)	513, 1440, 2018, 2611
FatI	CATG	8 cut(s)	1360, 1552, 1633, 1673, 1788, 1891, 2026, 2277
FbaI	TGATCA	3 cut(s)	313, 1594, 1636
Fnu4HI	GCNGC	5 cut(s)	109, 632, 1669, 2427, 2531
FokI	GGATG	9 cut(s)	194, 452, 1587, 1751, 2295, 2309, 2380, 2465, 2504
FriOI	GRGCYC	2 cut(s)	349, 1246
Fsp4HI	GCNGC	5 cut(s)	109, 632, 1669, 2427, 2531
FspBI	CTAG	6 cut(s)	428, 728, 804, 990, 1008, 2358
GlaI	GCGC	5 cut(s)	112, 165, 1233, 1667, 2084
GluI	GCNGC	5 cut(s)	109, 632, 1669, 2427, 2531
GsaI	CCCAGC	2 cut(s)	725, 2026
GsuI	CTGGAG	1 cut(s)	2341
HaeII	RGCGCY	4 cut(s)	114, 167, 1669, 2086
HaeIII	GGCC	4 cut(s)	230, 347, 1760, 2284
HapII	CCGG	3 cut(s)	105, 567, 1140
HgaI	GACGC	2 cut(s)	1316, 1578
HhaI	GCGC	5 cut(s)	113, 166, 1234, 1668, 2085
Hin1II	CATG	8 cut(s)	1364, 1556, 1637, 1677, 1792, 1895, 2030, 2281
Hin6I	GCGC	5 cut(s)	111, 164, 1232, 1666, 2083
HinP1I	GCGC	5 cut(s)	111, 164, 1232, 1666, 2083
HincII	GTYRAC	1 cut(s)	202
HindII	GTYRAC	1 cut(s)	202
HindIII	AAGCTT	1 cut(s)	1967
HpaII	CCGG	3 cut(s)	105, 567, 1140
HphI	GGTGA	6 cut(s)	25, 347, 539, 1090, 1352, 1830
Hpy166II	GTNNAC	5 cut(s)	202, 985, 1025, 1154, 2150
Hpy8I	GTNNAC	5 cut(s)	202, 985, 1025, 1154, 2150
Hpy99I	CGWCG	1 cut(s)	397
HpyAV	CCTTC	7 cut(s)	32, 549, 763, 872, 938, 1845, 2006
HpyCH4III	ACNGT	7 cut(s)	390, 643, 757, 836, 1484, 2602, 2621
HpyCH4IV	ACGT	4 cut(s)	270, 1429, 1557, 2092
HpyF10VI	GCNNNNNNNGC	5 cut(s)	110, 488, 964, 1677, 2432
HpySE526I	ACGT	4 cut(s)	270, 1429, 1557, 2092
Hsp92II	CATG	8 cut(s)	1364, 1556, 1637, 1677, 1792, 1895, 2030, 2281
HspAI	GCGC	5 cut(s)	111, 164, 1232, 1666, 2083
KpnI	GGTACC	1 cut(s)	1488
KroI	GCCGGC	1 cut(s)	104
KroNI	GCCGGC	1 cut(s)	106
Ksp22I	TGATCA	3 cut(s)	313, 1594, 1636
LguI	GCTCTTC	2 cut(s)	120, 128
LmnI	GCTCC	6 cut(s)	40, 161, 1241, 1249, 1630, 1663
Lsp1109I	GCAGC	4 cut(s)	643, 1655, 2438, 2517
LweI	GCATC	6 cut(s)	676, 1609, 2314, 2344, 2402, 2526
MaeI	CTAG	6 cut(s)	428, 728, 804, 990, 1008, 2358
MaeII	ACGT	4 cut(s)	270, 1429, 1557, 2092
MaeIII	GTNAC	8 cut(s)	172, 356, 448, 493, 1443, 1478, 2501, 2522
MfeI	CAATTG	1 cut(s)	1775
MflI	RGATCY	6 cut(s)	993, 1744, 1915, 2242, 2263, 2580
MhlI	GDGCHC	2 cut(s)	349, 1246
MlyI	GAGTC	3 cut(s)	456, 980, 1718
MmeI	TCCRAC	1 cut(s)	561
MreI	CGCCGGCG	1 cut(s)	104
MroNI	GCCGGC	1 cut(s)	104
MroXI	GAANNNNTTC	3 cut(s)	1056, 2070, 2700
MseI	TTAA	8 cut(s)	486, 1530, 1682, 1850, 1856, 2405, 2552, 2651
MslI	CAYNNNNRTG	3 cut(s)	939, 1890, 2157
MspCI	CTTAAG	1 cut(s)	2404
MspI	CCGG	3 cut(s)	105, 567, 1140
MspR9I	CCNGG	1 cut(s)	2585
MunI	CAATTG	1 cut(s)	1775
Mva1269I	GAATGC	2 cut(s)	1770, 2471
MvaI	CCWGG	1 cut(s)	2585
MwoI	GCNNNNNNNGC	5 cut(s)	110, 488, 964, 1677, 2432
NaeI	GCCGGC	1 cut(s)	106
NgoMIV	GCCGGC	1 cut(s)	104
NlaIII	CATG	8 cut(s)	1364, 1556, 1637, 1677, 1792, 1895, 2030, 2281
NlaIV	GGNNCC	8 cut(s)	244, 347, 561, 795, 1486, 1746, 2265, 2466
NmeAIII	GCCGAG	2 cut(s)	97, 1786
NmuCI	GTSAC	5 cut(s)	172, 356, 448, 493, 2522
NspI	RCATGY	1 cut(s)	2030
PaeI	GCATGC	1 cut(s)	2030
PaeR7I	CTCGAG	1 cut(s)	608
PagI	TCATGA	1 cut(s)	1552
PciSI	GCTCTTC	2 cut(s)	120, 128
PcsI	WCGNNNNNNNCGW	3 cut(s)	51, 1540, 2497
PctI	GAATGC	2 cut(s)	1770, 2471
PdiI	GCCGGC	1 cut(s)	106
PdmI	GAANNNNTTC	3 cut(s)	1056, 2070, 2700
PfeI	GAWTC	7 cut(s)	98, 815, 1052, 1343, 1573, 1579, 2637
PfoI	TCCNGGA	1 cut(s)	2583
PinAI	ACCGGT	1 cut(s)	566
PkrI	GCNGC	5 cut(s)	110, 633, 1670, 2428, 2532
Ple19I	CGATCG	1 cut(s)	11
PleI	GAGTC	3 cut(s)	455, 980, 1717
PmaCI	CACGTG	1 cut(s)	2093
PmlI	CACGTG	1 cut(s)	2093
PpsI	GAGTC	3 cut(s)	455, 980, 1717
Ppu21I	YACGTR	1 cut(s)	2093
PpuMI	RGGWCCY	1 cut(s)	881
PshBI	ATTAAT	2 cut(s)	1856, 2651
PsiI	TTATAA	1 cut(s)	2664
Psp124BI	GAGCTC	1 cut(s)	1246
Psp5II	RGGWCCY	1 cut(s)	881
Psp6I	CCWGG	1 cut(s)	2583
PspCI	CACGTG	1 cut(s)	2093
PspFI	CCCAGC	2 cut(s)	721, 2022
PspGI	CCWGG	1 cut(s)	2583
PspN4I	GGNNCC	8 cut(s)	244, 347, 561, 795, 1486, 1746, 2265, 2466
PspOMI	GGGCCC	1 cut(s)	345
PspPI	GGNCC	5 cut(s)	228, 345, 346, 881, 1352
PspPPI	RGGWCCY	1 cut(s)	881
PstI	CTGCAG	2 cut(s)	633, 2428
PstNI	CAGNNNCTG	3 cut(s)	1049, 1526, 2606
PsuI	RGATCY	6 cut(s)	993, 1744, 1915, 2242, 2263, 2580
PvuI	CGATCG	1 cut(s)	11
RsaI	GTAC	8 cut(s)	421, 640, 927, 1486, 1494, 2422, 2675, 2681
RsaNI	GTAC	8 cut(s)	420, 639, 926, 1485, 1493, 2421, 2674, 2680
RseI	CAYNNNNRTG	3 cut(s)	939, 1890, 2157
SacI	GAGCTC	1 cut(s)	1246
SapI	GCTCTTC	2 cut(s)	120, 128
SaqAI	TTAA	8 cut(s)	486, 1530, 1682, 1850, 1856, 2405, 2552, 2651
SatI	GCNGC	5 cut(s)	109, 632, 1669, 2427, 2531
Sau96I	GGNCC	5 cut(s)	228, 345, 346, 881, 1352
ScaI	AGTACT	3 cut(s)	421, 2422, 2681
SchI	GAGTC	3 cut(s)	456, 980, 1718
ScrFI	CCNGG	1 cut(s)	2585
SduI	GDGCHC	2 cut(s)	349, 1246
SfaNI	GCATC	6 cut(s)	676, 1609, 2314, 2344, 2402, 2526
SfcI	CTRYAG	5 cut(s)	629, 753, 2424, 2494, 2560
Sfr274I	CTCGAG	1 cut(s)	608
SgrAI	CRCCGGYG	2 cut(s)	104, 566
SinI	GGWCC	2 cut(s)	881, 1352
SlaI	CTCGAG	1 cut(s)	608
SmiMI	CAYNNNNRTG	3 cut(s)	939, 1890, 2157
SmlI	CTYRAG	4 cut(s)	608, 1496, 1716, 2404
SmoI	CTYRAG	4 cut(s)	608, 1496, 1716, 2404
SphI	GCATGC	1 cut(s)	2030
SsiI	CCGC	3 cut(s)	102, 108, 425
SspI	AATATT	1 cut(s)	1609
SspMI	CTAG	6 cut(s)	428, 728, 804, 990, 1008, 2358
SstI	GAGCTC	1 cut(s)	1246
StyD4I	CCNGG	1 cut(s)	2583
StyI	CCWWGG	1 cut(s)	1659
TaaI	ACNGT	7 cut(s)	390, 643, 757, 836, 1484, 2602, 2621
TaiI	ACGT	4 cut(s)	273, 1432, 1560, 2095
TaqI	TCGA	9 cut(s)	395, 573, 609, 697, 1264, 2172, 2616, 2635, 2655
TatI	WGTACW	6 cut(s)	419, 638, 1492, 2420, 2673, 2679
TauI	GCSGC	1 cut(s)	111
TfiI	GAWTC	7 cut(s)	98, 815, 1052, 1343, 1573, 1579, 2637
Tru1I	TTAA	8 cut(s)	486, 1530, 1682, 1850, 1856, 2405, 2552, 2651
Tru9I	TTAA	8 cut(s)	486, 1530, 1682, 1850, 1856, 2405, 2552, 2651
TscAI	CASTG	5 cut(s)	633, 762, 909, 1050, 2146
TseFI	GTSAC	5 cut(s)	172, 356, 448, 493, 2522
TseI	GCWGC	4 cut(s)	631, 1668, 2426, 2530
Tsp45I	GTSAC	5 cut(s)	172, 356, 448, 493, 2522
TspDTI	ATGAA	9 cut(s)	500, 957, 1341, 1389, 1476, 1908, 2331, 2400, 2469
TspGWI	ACGGA	2 cut(s)	1604, 2510
TspRI	CASTG	5 cut(s)	633, 762, 909, 1050, 2146
Vha464I	CTTAAG	1 cut(s)	2404
VpaK11BI	GGWCC	2 cut(s)	881, 1352
VspI	ATTAAT	2 cut(s)	1856, 2651
XapI	RAATTY	2 cut(s)	249, 2696
XbaI	TCTAGA	1 cut(s)	727
XceI	RCATGY	1 cut(s)	2030
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2030
XhoI	CTCGAG	1 cut(s)	608
XmnI	GAANNNNTTC	3 cut(s)	1056, 2070, 2700
XspI	CTAG	6 cut(s)	428, 728, 804, 990, 1008, 2358
ZrmI	AGTACT	3 cut(s)	421, 2422, 2681

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.