Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0007976

Physical Location & Seq

Genomic Coordinates Forward (+)

36103436 .. 36107684

Gene Structure 4249 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00243650.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2667 bp

ATGAGACGATCGGTGATCTTCCTTCTCCTATTTGCTCCCTTGATCGGCTGTTTCGTCAAGTCTGGTTCAGCTGAGGAGACCTCCCTCGCCAATCCAGATTCCGCCGGCGGCGCTGTTCAGCTCTTCAGCTCTCTTCTGCCTCCTGCACTTAGGAATGATGCAGCGCTTTTGGTCACATTGGATGGCAAGGTAGTTTCTGTCAACCCCAAGACGATGGAGATTGAGTGGGCCTTGTCCTCTGGTTCCCGAATTCACTCTTCCTATCACAACGCCTCTTATTTCAACAACTGCAGGAGTGATGATGATCGCTCTTTCGTTGATATTGGTGAAGACTGGGCCCTCTATCGTCACAGCAACACCAACACCATCAAGAAGAAGAAGAAGAAACTGTCGTCGAGTGTTAGAGAATATGTTGCAAGTACTCCGCTGGTTTCCGAGGATGGGGGAGTCACATTGGGGTCAAAGATCACTAAAGTATTTGCTGTTAATGCTGTGACCGGACAAGTAATTCATAGTTTTAGGTTGGGTGATAATACTCCTTCAGCTACTTTAGGTATGGAACCCACCGGTGCCGAGGGGTTGGAAACATCTGGACTGGTGCTTTATCTCGAGAGAACAGATTACACACTGCAGCATTGTACAGTCACGGGCGATCTTGTGTGGAAATTAGAAATTGCAGAGTTTGATGCTCATTTTCGATATCCTAAAATTGGAAATGGGCTTGGTCTAGAGGACAGGCTCATTGCTAACTCAACAGTGCCTTATCAGAAGGAGAAGCCTGTTGTCATTCGGGTTCCTTATCGTAACTCGGTGGAATCTCGTTCTGTAATTGACGGTCTAACTGATGGGTATTATGGAGGCAAACCTCTCCCCCTTGAAGGTCCTCATAATGATCTTCTGGCACTGCCTTTTGACAAAGATGCTGTACCTCGTCACAACAATGAAGGCAGAGAAATGTTTGCTTTGCCCTCTCTTGAATTTGTGGACTCTAGGATCTTGGCTCTCCCTAGTAGAGATGTTGGTAAACTGGACATCACAAGCTCAGTGGCTGAATCTATTACTAACTTCCCCATACAATCTCTTTTTGTATTTCTTCTCACCCTATTATCCATTCTGGGATATATCTTTCGCTTTCTGACTGGAAAACAGCGTAAACTGAAAGTGGTGGCTGAGGAAACTAAAGCGCAAGCAGGAGCTCCCAAAAAGAAGAAAACTCGAAGACTGGGAAATAATAAGAAGAATTTCAGTTATGAGAAAGACGCAAGAAATATGTCGTATGAATATAAGTTTGGTGAATCTAATGGACCTGCACATGGGGAAAGAAATGAAACAAAATTGCTCTTGACCTCTTCTGATTTTATTGATCGTGACACCGAGGGACGTAGGATTGGCAAGTTACTTGTTTCCAGTAATGAAATTGCTAAGGGAGTAACGGGTATCTATGATGGTCGTCCAGTAGCTGTTAAACGCCTTGTGCGAACTCATCATGATGTGGCTTCGAAAGAGATTCAGAATCTTATTGCGTCTGATCAGCATCCAAATATTGTCCGTTGGTATGGCGTGGAGCATGATCAAGATTTTGTCTATCTATCCTTGGAGCGCTGCTCATGTAGCTTAAATGACTTGATTTATTATTACTCAGAGTCTTCTCAAGGCCAAATTATCAACAAGGATGAGGATCCTCACTTTTTGGCCGAGTATCGCATTCAATTGCAGGCAATCATGGGAAAGAACAAGAATGTTGAATTGTGGAAGACAAATGGATACCCTTCACCCCAAATGTTAAAATTAATGAGTGATCTTGTTTCTGGACTTGCCCATTTGCATGAACTTGGAATTATACATCGGGATCTAAAGCCTCAGAATGTTTTGATAATCAAAGGCAGATCGTTATGTGCCAAGCTTTCAGATATGGGTATCAGCAAACGCCTTCAAGGGGACAGGTCTTCCATAACCCAGCATGCTACTGGTTATGGGAGTTCAGGTTGGCAAGCACCTGAACAACTTCTCCATCAGCGCCAAACACGTGCAGTAGATTTATTTAGTTTAGGTTGTCTCCTCTTTTTCTGTCTCACTGGGGGTAAACACCCTTATGGGGATAGTATTGAGCGTGATGTGAATATAGTGAATGATCGGAAAGACCTATTCTTGGTAGAGAATATACCAGAAGCAATGGATCTTTTTACTCGTCTCTTGGATCCCAACCCTGACATGAGGCCAACAGCTGTGGATGTGTTGCATCATCCCTTTTTCTGGAGTTCTGAGACGAGACTTTCATTTCTGCGCGATGCTAGTGATCGGGTTGAATTGGAGGATAGGGAGACTGCATCCCAACTCTTAAGTTCATTAGAAAGTACTGCAGCAGTTGCTCTAAATGGTAAGTGGGATGAAAAGATGGAACCTGCATTCCTCAATAACATTGGTCGCTACAGGCGTTACAAGTTTGACAGCATCCGTGACTTGCTGCGTGTAATAAGGAACAAGTTAAATCACTACAGAGAACTTCCCCAAGAGATCCAGGAATTATTGGGGACAGTTCCTGAGGGCTTCGACAGTTATTTTTCAAGTCGATTCCCTAAATTATTAATCGAGGTTTATAATGTGTTTTACAAGTACTGCAAAGGGGAGGAATTTTTTCGCAAGTATATACAAGGCAAATCGGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002376 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006983 GO:0006984 GO:0006986 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008380 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009816 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010467 GO:0010506 GO:0010508 GO:0012505 GO:0014070 GO:0016020 GO:0016070 GO:0019222 GO:0023052 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031984 GO:0033554 GO:0034263 GO:0034641 GO:0034976 GO:0035966 GO:0042175 GO:0042221 GO:0042493 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046483 GO:0046677 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071216 GO:0071704 GO:0090304 GO:0098542 GO:0098827 GO:1901360 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04140 ko04141 ko04210 ko04932 ko05010 map04140 map04141 map04210 map04932 map05010

Pfam Domains

Protein Families

PQQ Pkinase Ribonuc_2-5A

Protein Analysis

888

Amino Acids

100.01

Weight (kDa)

7.91

Isoelectric Point (pI)

39.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	482 - 751	3.6e-36	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	483 - 745	3.8e-25	Protein tyrosine and serine/threonine kinase
Ribonuc_2-5A	PF06479	757 - 882	4.4e-46	Ribonuclease 2-5A

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007976)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G24360 AT5G24360 AT5G24360
fragaria_vesca	FvH4_5g25750 FvH4_5g25750
malus_domestica	MD06G1007000.v1.1
prunus_persica	Prupe.5G008100_v2.0.a1 Prupe.5G008100_v2.0.a1 Prupe.5G008100_v2.0.a1
pyrus_communis	pycom02g08890 pycom06g00610
rosa_chinensis	RchiOBHm_Chr7g0216101
rosa_laevigata	RLG00000002594
rosa_multiflora	Rmu_co8312507.1_g000001 Rmu_sc0009767.1_g000002
rosa_roxburghii	Rroxscaffold_3G00243650
rosa_rugosa	Rorug07G0158900 Rorug07G0158900
rosa_samantha	Rh7AG295900 Rh7AG296000 Rh7BG288300 Rh7CG316300 Rh7DG298800
rosa_wichuraiana	Rw7G025170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2598
Acc36I	ACCTGC	2 cut(s)	1315, 2410
AccB1I	GGYRCC	1 cut(s)	569
AccII	CGCG	1 cut(s)	2286
AciI	CCGC	3 cut(s)	102, 108, 425
AclWI	GGATC	8 cut(s)	1001, 1673, 1686, 1857, 2184, 2192, 2205, 2509
AcoI	YGGCCR	1 cut(s)	1692
AcsI	RAATTY	4 cut(s)	249, 977, 1240, 2630
AcuI	CTGAAG	2 cut(s)	109, 525
AcvI	CACGTG	1 cut(s)	2027
AfaI	GTAC	5 cut(s)	421, 640, 927, 2356, 2615
AfeI	AGCGCT	2 cut(s)	165, 1601
AfiI	CCNNNNNNNGG	9 cut(s)	44, 441, 710, 878, 1313, 1936, 2095, 2149, 2253
AflII	CTTAAG	1 cut(s)	2338
AflIII	ACRYGT	1 cut(s)	2024
AgeI	ACCGGT	1 cut(s)	566
AgsI	TTSAA	8 cut(s)	283, 878, 977, 1709, 1745, 1934, 2306, 2565
AjnI	CCWGG	1 cut(s)	2517
AjuI	GAANNNNNNNTTGG	2 cut(s)	1272, 1304
AloI	GAACNNNNNNTCC	4 cut(s)	1992, 2024, 2391, 2423
Alw21I	GWGCWC	1 cut(s)	1198
Alw26I	GTCTC	7 cut(s)	71, 2060, 2075, 2195, 2258, 2263, 2315
AlwI	GGATC	8 cut(s)	1001, 1673, 1686, 1857, 2184, 2192, 2205, 2509
AlwNI	CAGNNNCTG	3 cut(s)	1049, 1460, 2540
Ama87I	CYCGRG	1 cut(s)	608
Aor51HI	AGCGCT	2 cut(s)	165, 1601
AoxI	GGCC	5 cut(s)	228, 336, 1654, 1692, 2216
ApaI	GGGCCC	1 cut(s)	340
ApeKI	GCWGC	5 cut(s)	161, 631, 1602, 2360, 2464
ApoI	RAATTY	4 cut(s)	249, 977, 1240, 2630
AseI	ATTAAT	2 cut(s)	1790, 2585
AsiGI	ACCGGT	1 cut(s)	566
Asp700I	GAANNNNTTC	3 cut(s)	1241, 2004, 2634
AspLEI	GCGC	6 cut(s)	113, 166, 1186, 1602, 2019, 2286
AspS9I	GGNCC	5 cut(s)	228, 336, 337, 881, 1304
AsuHPI	GGTGA	6 cut(s)	25, 338, 539, 1090, 1304, 1764
AsuII	TTCGAA	1 cut(s)	1499
AvaI	CYCGRG	1 cut(s)	608
AvaII	GGWCC	2 cut(s)	881, 1304
AxyI	CCTNAGG	1 cut(s)	2541
BaeGI	GKGCMC	1 cut(s)	340
BamHI	GGATCC	2 cut(s)	1678, 2197
BanI	GGYRCC	1 cut(s)	569
BanII	GRGCYC	2 cut(s)	340, 1198
BbrPI	CACGTG	1 cut(s)	2027
BbsI	GAAGAC	5 cut(s)	336, 1225, 1638, 1760, 1938
Bbv12I	GWGCWC	1 cut(s)	1198
BbvCI	CCTCAGC	2 cut(s)	72, 1170
BbvI	GCAGC	5 cut(s)	173, 643, 1589, 2372, 2451
BccI	CCATC	8 cut(s)	176, 208, 374, 434, 839, 1439, 2019, 2389
BciT130I	CCWGG	1 cut(s)	2519
BciVI	GTATCC	1 cut(s)	1757
BclI	TGATCA	2 cut(s)	1528, 1570
BcoDI	GTCTC	7 cut(s)	71, 2060, 2075, 2195, 2258, 2263, 2315
BfaI	CTAG	4 cut(s)	728, 990, 1008, 2292
BfmI	CTRYAG	5 cut(s)	289, 629, 2358, 2428, 2494
BfoI	RGCGCY	4 cut(s)	114, 167, 1603, 2020
BfrI	CTTAAG	1 cut(s)	2338
BfuAI	ACCTGC	2 cut(s)	1315, 2410
BfuI	GTATCC	1 cut(s)	1757
BisI	GCNGC	6 cut(s)	109, 162, 632, 1603, 2361, 2465
BlsI	GCNGC	6 cut(s)	110, 163, 633, 1604, 2362, 2466
BmcAI	AGTACT	3 cut(s)	421, 2356, 2615
Bme1390I	CCNGG	1 cut(s)	2519
Bme18I	GGWCC	2 cut(s)	881, 1304
BmeT110I	CYCGRG	1 cut(s)	608
BmgT120I	GGNCC	5 cut(s)	228, 336, 337, 881, 1304
BmiI	GGNNCC	8 cut(s)	244, 338, 561, 571, 795, 1680, 2199, 2400
BmrFI	CCNGG	1 cut(s)	2519
BmrI	ACTGGG	3 cut(s)	343, 1232, 2085
BmsI	GCATC	8 cut(s)	148, 676, 910, 1543, 2248, 2278, 2336, 2460
BmuI	ACTGGG	3 cut(s)	343, 1232, 2085
BpiI	GAAGAC	5 cut(s)	336, 1225, 1638, 1760, 1938
BplI	GAGNNNNNCTC	4 cut(s)	65, 97, 1589, 1621
BpmI	CTGGAG	1 cut(s)	2275
Bpu10I	CCTNAGC	3 cut(s)	72, 1170, 1422
Bpu14I	TTCGAA	1 cut(s)	1499
BpuEI	CTTGAG	1 cut(s)	1635
BsaAI	YACGTR	1 cut(s)	2027
BsaBI	GATNNNNATC	3 cut(s)	303, 1533, 1677
BsaI	GGTCTC	1 cut(s)	71
BsaJI	CCNNGG	4 cut(s)	435, 573, 1374, 1593
BsaWI	WCCGGW	2 cut(s)	497, 566
BsaXI	ACNNNNNCTCC	6 cut(s)	209, 239, 1969, 1992, 1999, 2022
Bsc4I	CCNNNNNNNGG	9 cut(s)	44, 441, 710, 878, 1313, 1936, 2095, 2149, 2253
Bse118I	RCCGGY	2 cut(s)	104, 566
Bse1I	ACTGG	9 cut(s)	338, 600, 1032, 1144, 1227, 1407, 1454, 1972, 2080
Bse21I	CCTNAGG	1 cut(s)	2541
Bse3DI	GCAATG	2 cut(s)	741, 2178
Bse8I	GATNNNNATC	3 cut(s)	303, 1533, 1677
BseBI	CCWGG	1 cut(s)	2519
BseDI	CCNNGG	4 cut(s)	435, 573, 1374, 1593
BseGI	GGATG	9 cut(s)	187, 445, 1534, 1678, 2236, 2242, 2327, 2392, 2451
BseJI	GATNNNNATC	3 cut(s)	303, 1533, 1677
BseLI	CCNNNNNNNGG	9 cut(s)	44, 441, 710, 878, 1313, 1936, 2095, 2149, 2253
BseMI	GCAATG	2 cut(s)	741, 2178
BseMII	CTCAG	7 cut(s)	63, 1056, 1161, 1653, 1874, 2253, 2532
BseNI	ACTGG	9 cut(s)	338, 600, 1032, 1144, 1227, 1407, 1454, 1972, 2080
BseRI	GAGGAG	2 cut(s)	89, 2048
BseSI	GKGCMC	1 cut(s)	340
BseXI	GCAGC	5 cut(s)	173, 643, 1589, 2372, 2451
BseYI	CCCAGC	1 cut(s)	1956
BsgI	GTGCAG	3 cut(s)	129, 1293, 2049
Bsh1236I	CGCG	1 cut(s)	2286
Bsh1285I	CGRYCG	1 cut(s)	11
BshFI	GGCC	5 cut(s)	230, 338, 1656, 1694, 2218
BshNI	GGYRCC	1 cut(s)	569
BshTI	ACCGGT	1 cut(s)	566
BsiEI	CGRYCG	1 cut(s)	11
BsiHKAI	GWGCWC	1 cut(s)	1198
BsiHKCI	CYCGRG	1 cut(s)	608
BsiSI	CCGG	3 cut(s)	105, 498, 567
BslFI	GGGAC	3 cut(s)	1392, 1952, 2545
BslI	CCNNNNNNNGG	9 cut(s)	44, 441, 710, 878, 1313, 1936, 2095, 2149, 2253
BsmAI	GTCTC	7 cut(s)	71, 2060, 2075, 2195, 2258, 2263, 2315
BsmBI	CGTCTC	2 cut(s)	2195, 2258
BsmFI	GGGAC	3 cut(s)	1392, 1952, 2545
BsmI	GAATGC	2 cut(s)	1704, 2405
BsnI	GGCC	5 cut(s)	230, 338, 1656, 1694, 2218
Bso31I	GGTCTC	1 cut(s)	71
BsoBI	CYCGRG	1 cut(s)	608
Bsp119I	TTCGAA	1 cut(s)	1499
Bsp120I	GGGCCC	1 cut(s)	336
Bsp1286I	GDGCHC	2 cut(s)	340, 1198
Bsp1407I	TGTACA	1 cut(s)	638
BspACI	CCGC	3 cut(s)	102, 108, 425
BspANI	GGCC	5 cut(s)	230, 338, 1656, 1694, 2218
BspCNI	CTCAG	7 cut(s)	64, 1055, 1162, 1652, 1873, 2254, 2533
BspFNI	CGCG	1 cut(s)	2286
BspHI	TCATGA	1 cut(s)	1486
BspLI	GGNNCC	8 cut(s)	244, 338, 561, 571, 795, 1680, 2199, 2400
BspMAI	CTGCAG	3 cut(s)	293, 633, 2362
BspMI	ACCTGC	2 cut(s)	1315, 2410
BspPI	GGATC	8 cut(s)	1001, 1673, 1686, 1857, 2184, 2192, 2205, 2509
BspQI	GCTCTTC	1 cut(s)	128
BspT104I	TTCGAA	1 cut(s)	1499
BspT107I	GGYRCC	1 cut(s)	569
BspTI	CTTAAG	1 cut(s)	2338
BspTNI	GGTCTC	1 cut(s)	71
BsrDI	GCAATG	2 cut(s)	741, 2178
BsrFI	RCCGGY	2 cut(s)	104, 566
BsrGI	TGTACA	1 cut(s)	638
BsrI	ACTGG	9 cut(s)	338, 600, 1032, 1144, 1227, 1407, 1454, 1972, 2080
BssAI	RCCGGY	2 cut(s)	104, 566
BssECI	CCNNGG	4 cut(s)	435, 573, 1374, 1593
BssT1I	CCWWGG	1 cut(s)	1593
Bst2UI	CCWGG	1 cut(s)	2519
Bst4CI	ACNGT	6 cut(s)	390, 643, 757, 836, 2536, 2555
Bst6I	CTCTTC	4 cut(s)	128, 138, 262, 1354
BstAFI	CTTAAG	1 cut(s)	2338
BstAPI	GCANNNNNTGC	1 cut(s)	2366
BstAUI	TGTACA	1 cut(s)	638
BstBAI	YACGTR	1 cut(s)	2027
BstBI	TTCGAA	1 cut(s)	1499
BstC8I	GCNNGC	5 cut(s)	106, 1188, 1716, 1962, 1992
BstDEI	CTNAG	9 cut(s)	72, 149, 1042, 1170, 1422, 1639, 1860, 2262, 2541
BstF5I	GGATG	9 cut(s)	187, 445, 1534, 1678, 2236, 2242, 2327, 2392, 2451
BstFNI	CGCG	1 cut(s)	2286
BstH2I	RGCGCY	4 cut(s)	114, 167, 1603, 2020
BstHHI	GCGC	6 cut(s)	113, 166, 1186, 1602, 2019, 2286
BstMAI	GTCTC	7 cut(s)	71, 2060, 2075, 2195, 2258, 2263, 2315
BstMCI	CGRYCG	1 cut(s)	11
BstMWI	GCNNNNNNNGC	4 cut(s)	110, 488, 1611, 2366
BstNI	CCWGG	1 cut(s)	2519
BstNSI	RCATGY	1 cut(s)	1964
BstSCI	CCNGG	1 cut(s)	2517
BstSFI	CTRYAG	5 cut(s)	289, 629, 2358, 2428, 2494
BstSLI	GKGCMC	1 cut(s)	340
BstUI	CGCG	1 cut(s)	2286
BstV1I	GCAGC	5 cut(s)	173, 643, 1589, 2372, 2451
BstV2I	GAAGAC	5 cut(s)	336, 1225, 1638, 1760, 1938
BstX2I	RGATCY	6 cut(s)	993, 1678, 1849, 2176, 2197, 2514
BstXI	CCANNNNNNTGG	1 cut(s)	214
BstYI	RGATCY	6 cut(s)	993, 1678, 1849, 2176, 2197, 2514
Bsu36I	CCTNAGG	1 cut(s)	2541
BsuI	GTATCC	1 cut(s)	1757
BsuRI	GGCC	5 cut(s)	230, 338, 1656, 1694, 2218
BtgZI	GCGATG	1 cut(s)	2301
BtsCI	GGATG	9 cut(s)	187, 445, 1534, 1678, 2236, 2242, 2327, 2392, 2451
BtsI	GCAGTG	2 cut(s)	626, 902
BtsIMutI	CAGTG	5 cut(s)	626, 762, 902, 1050, 2073
BveI	ACCTGC	2 cut(s)	1315, 2410
Cac8I	GCNNGC	5 cut(s)	106, 1188, 1716, 1962, 1992
CaiI	CAGNNNCTG	3 cut(s)	1049, 1460, 2540
CciI	TCATGA	1 cut(s)	1486
CfoI	GCGC	6 cut(s)	113, 166, 1186, 1602, 2019, 2286
Cfr10I	RCCGGY	2 cut(s)	104, 566
Cfr13I	GGNCC	5 cut(s)	228, 336, 337, 881, 1304
CseI	GACGC	2 cut(s)	1268, 1512
Csp6I	GTAC	5 cut(s)	420, 639, 926, 2355, 2614
CspAI	ACCGGT	1 cut(s)	566
CspCI	CAANNNNNGTGG	4 cut(s)	1026, 1061, 2208, 2243
CviAII	CATG	8 cut(s)	1313, 1487, 1568, 1608, 1723, 1826, 1961, 2212
CviQI	GTAC	5 cut(s)	420, 639, 926, 2355, 2614
DdeI	CTNAG	9 cut(s)	72, 149, 1042, 1170, 1422, 1639, 1860, 2262, 2541
EaeI	YGGCCR	1 cut(s)	1692
Eam1104I	CTCTTC	4 cut(s)	128, 138, 262, 1354
EarI	CTCTTC	4 cut(s)	128, 138, 262, 1354
EciI	GGCGGA	1 cut(s)	91
Ecl136II	GAGCTC	1 cut(s)	1196
Eco130I	CCWWGG	1 cut(s)	1593
Eco24I	GRGCYC	2 cut(s)	340, 1198
Eco31I	GGTCTC	1 cut(s)	71
Eco32I	GATATC	1 cut(s)	701
Eco47I	GGWCC	2 cut(s)	881, 1304
Eco47III	AGCGCT	2 cut(s)	165, 1601
Eco53kI	GAGCTC	1 cut(s)	1196
Eco57I	CTGAAG	2 cut(s)	109, 525
Eco72I	CACGTG	1 cut(s)	2027
Eco81I	CCTNAGG	1 cut(s)	2541
Eco88I	CYCGRG	1 cut(s)	608
EcoICRI	GAGCTC	1 cut(s)	1196
EcoO109I	RGGNCCY	2 cut(s)	337, 881
EcoRI	GAATTC	1 cut(s)	249
EcoRII	CCWGG	1 cut(s)	2517
EcoRV	GATATC	1 cut(s)	701
EcoT14I	CCWWGG	1 cut(s)	1593
EcoT38I	GRGCYC	2 cut(s)	340, 1198
ErhI	CCWWGG	1 cut(s)	1593
Esp3I	CGTCTC	2 cut(s)	2195, 2258
FaeI	CATG	8 cut(s)	1316, 1490, 1571, 1611, 1726, 1829, 1964, 2215
FaqI	GGGAC	3 cut(s)	1392, 1952, 2545
FatI	CATG	8 cut(s)	1312, 1486, 1567, 1607, 1722, 1825, 1960, 2211
FbaI	TGATCA	2 cut(s)	1528, 1570
Fnu4HI	GCNGC	6 cut(s)	109, 162, 632, 1603, 2361, 2465
FokI	GGATG	9 cut(s)	194, 452, 1521, 1685, 2229, 2243, 2314, 2399, 2438
FriOI	GRGCYC	2 cut(s)	340, 1198
Fsp4HI	GCNGC	6 cut(s)	109, 162, 632, 1603, 2361, 2465
FspBI	CTAG	4 cut(s)	728, 990, 1008, 2292
GlaI	GCGC	6 cut(s)	112, 165, 1185, 1601, 2018, 2285
GluI	GCNGC	6 cut(s)	109, 162, 632, 1603, 2361, 2465
GsaI	CCCAGC	1 cut(s)	1960
GsuI	CTGGAG	1 cut(s)	2275
HaeII	RGCGCY	4 cut(s)	114, 167, 1603, 2020
HaeIII	GGCC	5 cut(s)	230, 338, 1656, 1694, 2218
HapII	CCGG	3 cut(s)	105, 498, 567
HgaI	GACGC	2 cut(s)	1268, 1512
HhaI	GCGC	6 cut(s)	113, 166, 1186, 1602, 2019, 2286
Hin1II	CATG	8 cut(s)	1316, 1490, 1571, 1611, 1726, 1829, 1964, 2215
Hin6I	GCGC	6 cut(s)	111, 164, 1184, 1600, 2017, 2284
HinP1I	GCGC	6 cut(s)	111, 164, 1184, 1600, 2017, 2284
HincII	GTYRAC	1 cut(s)	202
HindII	GTYRAC	1 cut(s)	202
HindIII	AAGCTT	1 cut(s)	1901
HpaII	CCGG	3 cut(s)	105, 498, 567
HphI	GGTGA	6 cut(s)	25, 338, 539, 1090, 1304, 1764
Hpy166II	GTNNAC	5 cut(s)	202, 985, 1025, 1154, 2084
Hpy8I	GTNNAC	5 cut(s)	202, 985, 1025, 1154, 2084
Hpy99I	CGWCG	1 cut(s)	397
HpyAV	CCTTC	7 cut(s)	32, 549, 763, 872, 938, 1779, 1940
HpyCH4III	ACNGT	6 cut(s)	390, 643, 757, 836, 2536, 2555
HpyCH4IV	ACGT	2 cut(s)	1381, 2026
HpyF10VI	GCNNNNNNNGC	4 cut(s)	110, 488, 1611, 2366
HpyF3I	CTNAG	9 cut(s)	72, 149, 1042, 1170, 1422, 1639, 1860, 2262, 2541
HpySE526I	ACGT	2 cut(s)	1381, 2026
Hsp92II	CATG	8 cut(s)	1316, 1490, 1571, 1611, 1726, 1829, 1964, 2215
HspAI	GCGC	6 cut(s)	111, 164, 1184, 1600, 2017, 2284
KroI	GCCGGC	1 cut(s)	104
KroNI	GCCGGC	1 cut(s)	106
Ksp22I	TGATCA	2 cut(s)	1528, 1570
LguI	GCTCTTC	1 cut(s)	128
LmnI	GCTCC	5 cut(s)	40, 1193, 1201, 1564, 1597
Lsp1109I	GCAGC	5 cut(s)	173, 643, 1589, 2372, 2451
LweI	GCATC	8 cut(s)	148, 676, 910, 1543, 2248, 2278, 2336, 2460
MaeI	CTAG	4 cut(s)	728, 990, 1008, 2292
MaeII	ACGT	2 cut(s)	1381, 2026
MfeI	CAATTG	1 cut(s)	1709
MflI	RGATCY	6 cut(s)	993, 1678, 1849, 2176, 2197, 2514
MhlI	GDGCHC	2 cut(s)	340, 1198
MlyI	GAGTC	3 cut(s)	456, 980, 1652
MmeI	TCCRAC	1 cut(s)	561
MreI	CGCCGGCG	1 cut(s)	104
MroNI	GCCGGC	1 cut(s)	104
MroXI	GAANNNNTTC	3 cut(s)	1241, 2004, 2634
MseI	TTAA	8 cut(s)	486, 1464, 1616, 1784, 1790, 2339, 2486, 2585
MslI	CAYNNNNRTG	4 cut(s)	939, 1488, 1824, 2091
MspA1I	CMGCKG	3 cut(s)	71, 427, 2225
MspCI	CTTAAG	1 cut(s)	2338
MspI	CCGG	3 cut(s)	105, 498, 567
MspR9I	CCNGG	1 cut(s)	2519
MunI	CAATTG	1 cut(s)	1709
Mva1269I	GAATGC	2 cut(s)	1704, 2405
MvaI	CCWGG	1 cut(s)	2519
MvnI	CGCG	1 cut(s)	2286
MwoI	GCNNNNNNNGC	4 cut(s)	110, 488, 1611, 2366
NaeI	GCCGGC	1 cut(s)	106
NgoMIV	GCCGGC	1 cut(s)	104
NlaIII	CATG	8 cut(s)	1316, 1490, 1571, 1611, 1726, 1829, 1964, 2215
NlaIV	GGNNCC	8 cut(s)	244, 338, 561, 571, 795, 1680, 2199, 2400
NmeAIII	GCCGAG	2 cut(s)	598, 1720
NmuCI	GTSAC	8 cut(s)	172, 347, 448, 493, 643, 932, 1367, 2456
NspI	RCATGY	1 cut(s)	1964
NspV	TTCGAA	1 cut(s)	1499
PaeI	GCATGC	1 cut(s)	1964
PaeR7I	CTCGAG	1 cut(s)	608
PagI	TCATGA	1 cut(s)	1486
PciSI	GCTCTTC	1 cut(s)	128
PcsI	WCGNNNNNNNCGW	3 cut(s)	51, 1474, 2431
PctI	GAATGC	2 cut(s)	1704, 2405
PdiI	GCCGGC	1 cut(s)	106
PdmI	GAANNNNTTC	3 cut(s)	1241, 2004, 2634
PfeI	GAWTC	7 cut(s)	98, 815, 1052, 1295, 1507, 1513, 2571
PfoI	TCCNGGA	1 cut(s)	2517
PinAI	ACCGGT	1 cut(s)	566
PkrI	GCNGC	6 cut(s)	110, 163, 633, 1604, 2362, 2466
Ple19I	CGATCG	1 cut(s)	11
PleI	GAGTC	3 cut(s)	455, 980, 1651
PmaCI	CACGTG	1 cut(s)	2027
PmlI	CACGTG	1 cut(s)	2027
PpsI	GAGTC	3 cut(s)	455, 980, 1651
Ppu21I	YACGTR	1 cut(s)	2027
PpuMI	RGGWCCY	1 cut(s)	881
PshBI	ATTAAT	2 cut(s)	1790, 2585
PsiI	TTATAA	1 cut(s)	2598
Psp124BI	GAGCTC	1 cut(s)	1198
Psp5II	RGGWCCY	1 cut(s)	881
Psp6I	CCWGG	1 cut(s)	2517
PspCI	CACGTG	1 cut(s)	2027
PspFI	CCCAGC	1 cut(s)	1956
PspGI	CCWGG	1 cut(s)	2517
PspN4I	GGNNCC	8 cut(s)	244, 338, 561, 571, 795, 1680, 2199, 2400
PspOMI	GGGCCC	1 cut(s)	336
PspPI	GGNCC	5 cut(s)	228, 336, 337, 881, 1304
PspPPI	RGGWCCY	1 cut(s)	881
PstI	CTGCAG	3 cut(s)	293, 633, 2362
PstNI	CAGNNNCTG	3 cut(s)	1049, 1460, 2540
PsuI	RGATCY	6 cut(s)	993, 1678, 1849, 2176, 2197, 2514
PvuI	CGATCG	1 cut(s)	11
PvuII	CAGCTG	2 cut(s)	71, 2225
RsaI	GTAC	5 cut(s)	421, 640, 927, 2356, 2615
RsaNI	GTAC	5 cut(s)	420, 639, 926, 2355, 2614
RseI	CAYNNNNRTG	4 cut(s)	939, 1488, 1824, 2091
SacI	GAGCTC	1 cut(s)	1198
SapI	GCTCTTC	1 cut(s)	128
SaqAI	TTAA	8 cut(s)	486, 1464, 1616, 1784, 1790, 2339, 2486, 2585
SatI	GCNGC	6 cut(s)	109, 162, 632, 1603, 2361, 2465
Sau96I	GGNCC	5 cut(s)	228, 336, 337, 881, 1304
ScaI	AGTACT	3 cut(s)	421, 2356, 2615
SchI	GAGTC	3 cut(s)	456, 980, 1652
ScrFI	CCNGG	1 cut(s)	2519
SduI	GDGCHC	2 cut(s)	340, 1198
SfaNI	GCATC	8 cut(s)	148, 676, 910, 1543, 2248, 2278, 2336, 2460
SfcI	CTRYAG	5 cut(s)	289, 629, 2358, 2428, 2494
Sfr274I	CTCGAG	1 cut(s)	608
SfuI	TTCGAA	1 cut(s)	1499
SgrAI	CRCCGGYG	2 cut(s)	104, 566
SinI	GGWCC	2 cut(s)	881, 1304
SlaI	CTCGAG	1 cut(s)	608
SmiMI	CAYNNNNRTG	4 cut(s)	939, 1488, 1824, 2091
SmlI	CTYRAG	3 cut(s)	608, 1650, 2338
SmoI	CTYRAG	3 cut(s)	608, 1650, 2338
SphI	GCATGC	1 cut(s)	1964
SsiI	CCGC	3 cut(s)	102, 108, 425
SspI	AATATT	1 cut(s)	1543
SspMI	CTAG	4 cut(s)	728, 990, 1008, 2292
SstI	GAGCTC	1 cut(s)	1198
StyD4I	CCNGG	1 cut(s)	2517
StyI	CCWWGG	1 cut(s)	1593
TaaI	ACNGT	6 cut(s)	390, 643, 757, 836, 2536, 2555
TaiI	ACGT	2 cut(s)	1384, 2029
TaqI	TCGA	8 cut(s)	395, 609, 697, 1216, 1499, 2550, 2569, 2589
TatI	WGTACW	4 cut(s)	419, 638, 2354, 2613
TauI	GCSGC	1 cut(s)	111
TfiI	GAWTC	7 cut(s)	98, 815, 1052, 1295, 1507, 1513, 2571
Tru1I	TTAA	8 cut(s)	486, 1464, 1616, 1784, 1790, 2339, 2486, 2585
Tru9I	TTAA	8 cut(s)	486, 1464, 1616, 1784, 1790, 2339, 2486, 2585
TscAI	CASTG	5 cut(s)	633, 762, 909, 1050, 2080
TseFI	GTSAC	8 cut(s)	172, 347, 448, 493, 643, 932, 1367, 2456
TseI	GCWGC	5 cut(s)	161, 631, 1602, 2360, 2464
Tsp45I	GTSAC	8 cut(s)	172, 347, 448, 493, 643, 932, 1367, 2456
TspDTI	ATGAA	9 cut(s)	500, 957, 1293, 1341, 1428, 1842, 2265, 2334, 2403
TspGWI	ACGGA	2 cut(s)	1538, 2444
TspRI	CASTG	5 cut(s)	633, 762, 909, 1050, 2080
Vha464I	CTTAAG	1 cut(s)	2338
VpaK11BI	GGWCC	2 cut(s)	881, 1304
VspI	ATTAAT	2 cut(s)	1790, 2585
XapI	RAATTY	4 cut(s)	249, 977, 1240, 2630
XbaI	TCTAGA	1 cut(s)	727
XceI	RCATGY	1 cut(s)	1964
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1964
XhoI	CTCGAG	1 cut(s)	608
XmnI	GAANNNNTTC	3 cut(s)	1241, 2004, 2634
XspI	CTAG	4 cut(s)	728, 990, 1008, 2292
ZrmI	AGTACT	3 cut(s)	421, 2356, 2615

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.