Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0007976

Physical Location & Seq

Genomic Coordinates Reverse (-)

31457082 .. 31461599

Gene Structure 4518 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G025170.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2130 bp

ATGGAACCCACTGGTGCCGAGGGGTTGGAAACATCTGGACTGGTGTTGTATCTCGAGAGAACAGATTACACACTGCAGCATTGTACAGTCAAGGGCGATCTTCTGTGGAAATTAGAAATTGCAGAGTTTGATGCTCACTTTCGATATCCTAAAATTGGAAATGGGCTTGGTCCAGAGGACAGGCTTATTGCTAACTCAACAGTGCCTTATCAGAAGGAGAAGCCTGTTGTCATTCGCGTTCCTTATCCTAATTCGGTGGAATCTCGTTCTGTAATTGACGGTCTAACTGATGGGTATTTTGGAGGCAAACCTCTCCCCCTTGAAGGTCCTCATAACGATCTTCTGGCACTGCCTTTTGACAAAGGTGCTGTACCTCCTCACAACAATGAAGGCAGAGAAATGCTTGCTTTGCCCTCTCTTGAATTTGTGGACTCTAGGATCTTGGCTCTCCCTAGTAGAGATGTTGGTAAACTGGACACCACAAGCTCTGTGGCTGAATCTATTACTAACTTCCCCATACAATCTCTTTTTGTATTTCTTCTCACCCTATTATCCATTCTGGGATATATCTTTCGTTTTCTGACTGGAAAACAGCGTAAACTGAAAGTGGTGGCTGAGGAAACTAAAGCGCAAGCAGGAGCTCCCAAAAAGAAGAAAACTCGAAGATTGGGAAATAATAAGAAGAATTTCAGTTATGAGAAAGACGCAAGAAATATGTCGTATGAATATAAGTTTGGTGAATCTAATGGACCTGCCCATGGGGAAAGAAATGAAAAAAAATTGCTCTTGACCTCTTCTGATTTTATTGATCGTGACACCGAGGGACGTAGGATTGGCAAGTTACTTGTTTCCAGTAATGAAATTGCTAAGGGGAGTAACGGTACCATTGTACTTGAGGGTATCTATGATGGTCGTCCAGTAGCTGTTAAACGCCTTGTGCGAACTCATCATGATGTGGCTTTGAAAGAGATTCAGAATCTTATTGCGTCTGATCAGCATCCAAATATTGTCCGTTGGTATGGCGTGGAGCATGATCAAGATTTTGTCTATCTATCCTTGGAGCGCTGCTCATGTAGCTTAAATGACTTGATTTATTATTACTCAGAGTCTTCTCAAGGCCAAATTATCAACAAGGATGAGGATCCTCACTTTTTGGCCGAGTATCGCATTCAATTGCAGGCAATCATGGGAAAGAACAAGAATGTTGAATTGTGGAAGACAAATGGATACCCTTCACCCCAAATGTTAAAATTAATGAGTGATCTTGTTTCTGGACTTGCCCATTTGCATGAACTTGGAATTATACATCGGGATCTAAAGCCTCAGAATGTTTTGATAATCAAAGGCAGATCGTTATGTGCCAAGCTTTCAGATATGGGTATCAGCAAACGCCTTCAAGGGGACAGGTCTTCCATAACCCAGCATGCTACTGGTTATGGGAGTTCAGGTTGGCAAGCACCTGAACAACTTCTCCATCAGCGGCAAACACGTGCAGTAGATTTATTTAGTTTAGGTTGTCTCCTCTTTTTCTGTCTCACTGGGGGTAAACACCCTTATGGGGATAGTATCGAGCGTGATGTGAATATAGTGAATGATCGGAAAGACCTATTCTTGGTAGAGAATATACCAGAAGCAATGGATCTTTTTACTCGTCTCTTGGATCCCAACCCTGACATGAGGCCAACAGCTGTGGATGTGTTGCATCATCCCTTTTTCTGGAGTTCTGAGACGAGACTTTCGTTTCTGCGTGATGCTAGTGATCGGGTTGAATTGGAGGATAGGGAGACTGCATCCCAGCTCTTAAGTTCATTAGAAAGTACTGCAGCAGTTGCTCTAAATGGTAAGTGGGATGAAAAGATGGAACCTGCATTCCTCAATAACATTGGTCGCTACAGGCGTTACAAGTTTGACAGCATCCGTGACTTGCTGCGTGTAATAAGGAACAAGTTAAATCACTACAGAGAACTTCCCCAAGAGATCCAGGAATTATTGGGGACAGTTCCTGAGGGCTTCGACAGTTATTTTTCAAGTCGATTCCCTAAATTATTAATCGAGGTTTATAATGTGTTGTACAAGTCCTGCAAAGAGGAGGAATTTTTTCGCAAGTATATACAAGGCAAATTGGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002376 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006983 GO:0006984 GO:0006986 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008380 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009816 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010467 GO:0010506 GO:0010508 GO:0012505 GO:0014070 GO:0016020 GO:0016070 GO:0019222 GO:0023052 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031984 GO:0033554 GO:0034263 GO:0034641 GO:0034976 GO:0035966 GO:0042175 GO:0042221 GO:0042493 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046483 GO:0046677 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071216 GO:0071704 GO:0090304 GO:0098542 GO:0098827 GO:1901360 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04140 ko04141 ko04210 ko04932 ko05010 map04140 map04141 map04210 map04932 map05010

Pfam Domains

Protein Families

PQQ Pkinase Ribonuc_2-5A

Protein Analysis

709

Amino Acids

80.54

Weight (kDa)

6.85

Isoelectric Point (pI)

40.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	285 - 566	3.1e-27	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	286 - 572	1.8e-37	Protein kinase domain
Ribonuc_2-5A	PF06479	578 - 703	1.2e-46	Ribonuclease 2-5A

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007976)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G24360 AT5G24360 AT5G24360
fragaria_vesca	FvH4_5g25750 FvH4_5g25750
malus_domestica	MD06G1007000.v1.1
prunus_persica	Prupe.5G008100_v2.0.a1 Prupe.5G008100_v2.0.a1 Prupe.5G008100_v2.0.a1
pyrus_communis	pycom02g08890 pycom06g00610
rosa_chinensis	RchiOBHm_Chr7g0216101
rosa_laevigata	RLG00000002594
rosa_multiflora	Rmu_co8312507.1_g000001 Rmu_sc0009767.1_g000002
rosa_roxburghii	Rroxscaffold_3G00243650
rosa_rugosa	Rorug07G0158900 Rorug07G0158900
rosa_samantha	Rh7AG295900 Rh7AG296000 Rh7BG288300 Rh7CG316300 Rh7DG298800
rosa_wichuraiana	Rw7G025170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2061
Acc36I	ACCTGC	2 cut(s)	760, 1873
Acc65I	GGTACC	1 cut(s)	881
AccB1I	GGYRCC	2 cut(s)	14, 881
AccII	CGCG	1 cut(s)	237
AciI	CCGC	1 cut(s)	1480
AclWI	GGATC	8 cut(s)	446, 1136, 1149, 1320, 1647, 1655, 1668, 1972
AcoI	YGGCCR	1 cut(s)	1155
AcsI	RAATTY	3 cut(s)	422, 685, 2093
AcvI	CACGTG	1 cut(s)	1490
AfaI	GTAC	6 cut(s)	85, 372, 883, 891, 1819, 2072
AfeI	AGCGCT	1 cut(s)	1064
AfiI	CCNNNNNNNGG	7 cut(s)	155, 323, 758, 1399, 1558, 1612, 1716
AflII	CTTAAG	1 cut(s)	1801
AflIII	ACRYGT	1 cut(s)	1487
AgsI	TTSAA	8 cut(s)	323, 422, 964, 1172, 1208, 1397, 1769, 2028
AjnI	CCWGG	1 cut(s)	1980
AjuI	GAANNNNNNNTTGG	2 cut(s)	717, 749
AloI	GAACNNNNNNTCC	4 cut(s)	1455, 1487, 1854, 1886
AluBI	AGCT	7 cut(s)	486, 641, 923, 1077, 1366, 1688, 1798
AluI	AGCT	7 cut(s)	486, 641, 923, 1077, 1366, 1688, 1798
Alw21I	GWGCWC	1 cut(s)	643
Alw26I	GTCTC	6 cut(s)	1523, 1538, 1658, 1721, 1726, 1778
AlwI	GGATC	8 cut(s)	446, 1136, 1149, 1320, 1647, 1655, 1668, 1972
AlwNI	CAGNNNCTG	2 cut(s)	923, 2003
Ama87I	CYCGRG	1 cut(s)	53
Aor51HI	AGCGCT	1 cut(s)	1064
AoxI	GGCC	3 cut(s)	1117, 1155, 1679
ApeKI	GCWGC	4 cut(s)	76, 1065, 1823, 1927
ApoI	RAATTY	3 cut(s)	422, 685, 2093
ArsI	GACNNNNNNTTYG	2 cut(s)	1720, 1752
AseI	ATTAAT	2 cut(s)	1253, 2048
Asp700I	GAANNNNTTC	3 cut(s)	686, 1467, 2097
Asp718I	GGTACC	1 cut(s)	881
AspLEI	GCGC	2 cut(s)	631, 1065
AspS9I	GGNCC	3 cut(s)	170, 326, 749
AsuHPI	GGTGA	3 cut(s)	535, 749, 1227
AvaI	CYCGRG	1 cut(s)	53
AvaII	GGWCC	3 cut(s)	170, 326, 749
AxyI	CCTNAGG	1 cut(s)	2004
BaeI	ACNNNNGTAYC	2 cut(s)	873, 906
BamHI	GGATCC	2 cut(s)	1141, 1660
BanI	GGYRCC	2 cut(s)	14, 881
BanII	GRGCYC	1 cut(s)	643
BbrPI	CACGTG	1 cut(s)	1490
BbsI	GAAGAC	3 cut(s)	1101, 1223, 1401
Bbv12I	GWGCWC	1 cut(s)	643
BbvCI	CCTCAGC	1 cut(s)	615
BbvI	GCAGC	4 cut(s)	88, 1052, 1835, 1914
BccI	CCATC	4 cut(s)	284, 902, 1482, 1852
BciT130I	CCWGG	1 cut(s)	1982
BciVI	GTATCC	1 cut(s)	1220
BclI	TGATCA	2 cut(s)	991, 1033
BcoDI	GTCTC	6 cut(s)	1523, 1538, 1658, 1721, 1726, 1778
BfaI	CTAG	3 cut(s)	435, 453, 1755
BfmI	CTRYAG	4 cut(s)	74, 1821, 1891, 1957
BfoI	RGCGCY	1 cut(s)	1066
BfrI	CTTAAG	1 cut(s)	1801
BfuAI	ACCTGC	2 cut(s)	760, 1873
BfuI	GTATCC	1 cut(s)	1220
BisI	GCNGC	5 cut(s)	77, 1066, 1481, 1824, 1928
BlsI	GCNGC	5 cut(s)	78, 1067, 1482, 1825, 1929
BmcAI	AGTACT	1 cut(s)	1819
Bme1390I	CCNGG	1 cut(s)	1982
Bme18I	GGWCC	3 cut(s)	170, 326, 749
BmeT110I	CYCGRG	1 cut(s)	53
BmgT120I	GGNCC	3 cut(s)	170, 326, 749
BmiI	GGNNCC	6 cut(s)	6, 16, 883, 1143, 1662, 1863
BmrFI	CCNGG	1 cut(s)	1982
BmrI	ACTGGG	1 cut(s)	1548
BmsI	GCATC	6 cut(s)	121, 1006, 1711, 1741, 1799, 1923
BmuI	ACTGGG	1 cut(s)	1548
BpiI	GAAGAC	3 cut(s)	1101, 1223, 1401
BplI	GAGNNNNNCTC	2 cut(s)	1052, 1084
BpmI	CTGGAG	1 cut(s)	1738
Bpu10I	CCTNAGC	2 cut(s)	615, 867
BpuEI	CTTGAG	2 cut(s)	914, 1098
BsaAI	YACGTR	1 cut(s)	1490
BsaBI	GATNNNNATC	2 cut(s)	996, 1140
BsaJI	CCNNGG	4 cut(s)	18, 757, 819, 1056
BsaXI	ACNNNNNCTCC	6 cut(s)	865, 895, 1432, 1455, 1462, 1485
Bsc4I	CCNNNNNNNGG	7 cut(s)	155, 323, 758, 1399, 1558, 1612, 1716
Bse1I	ACTGG	8 cut(s)	16, 45, 477, 589, 852, 917, 1435, 1543
Bse21I	CCTNAGG	1 cut(s)	2004
Bse3DI	GCAATG	1 cut(s)	1641
Bse8I	GATNNNNATC	2 cut(s)	996, 1140
BseBI	CCWGG	1 cut(s)	1982
BseDI	CCNNGG	4 cut(s)	18, 757, 819, 1056
BseGI	GGATG	7 cut(s)	997, 1141, 1699, 1705, 1790, 1855, 1914
BseJI	GATNNNNATC	2 cut(s)	996, 1140
BseLI	CCNNNNNNNGG	7 cut(s)	155, 323, 758, 1399, 1558, 1612, 1716
BseMI	GCAATG	1 cut(s)	1641
BseMII	CTCAG	5 cut(s)	606, 1116, 1337, 1716, 1995
BseNI	ACTGG	8 cut(s)	16, 45, 477, 589, 852, 917, 1435, 1543
BseRI	GAGGAG	3 cut(s)	366, 1511, 2102
BseXI	GCAGC	4 cut(s)	88, 1052, 1835, 1914
BseYI	CCCAGC	2 cut(s)	1419, 1794
BsgI	GTGCAG	1 cut(s)	1512
Bsh1236I	CGCG	1 cut(s)	237
BshFI	GGCC	3 cut(s)	1119, 1157, 1681
BshNI	GGYRCC	2 cut(s)	14, 881
BsiHKAI	GWGCWC	1 cut(s)	643
BsiHKCI	CYCGRG	1 cut(s)	53
BslFI	GGGAC	3 cut(s)	837, 1415, 2008
BslI	CCNNNNNNNGG	7 cut(s)	155, 323, 758, 1399, 1558, 1612, 1716
BsmAI	GTCTC	6 cut(s)	1523, 1538, 1658, 1721, 1726, 1778
BsmBI	CGTCTC	2 cut(s)	1658, 1721
BsmFI	GGGAC	3 cut(s)	837, 1415, 2008
BsmI	GAATGC	2 cut(s)	1167, 1868
BsnI	GGCC	3 cut(s)	1119, 1157, 1681
BsoBI	CYCGRG	1 cut(s)	53
Bsp1286I	GDGCHC	1 cut(s)	643
Bsp1407I	TGTACA	2 cut(s)	83, 2070
Bsp19I	CCATGG	1 cut(s)	757
BspACI	CCGC	1 cut(s)	1480
BspANI	GGCC	3 cut(s)	1119, 1157, 1681
BspCNI	CTCAG	5 cut(s)	607, 1115, 1336, 1717, 1996
BspFNI	CGCG	1 cut(s)	237
BspHI	TCATGA	1 cut(s)	949
BspLI	GGNNCC	6 cut(s)	6, 16, 883, 1143, 1662, 1863
BspMAI	CTGCAG	2 cut(s)	78, 1825
BspMI	ACCTGC	2 cut(s)	760, 1873
BspPI	GGATC	8 cut(s)	446, 1136, 1149, 1320, 1647, 1655, 1668, 1972
BspT107I	GGYRCC	2 cut(s)	14, 881
BspTI	CTTAAG	1 cut(s)	1801
BsrDI	GCAATG	1 cut(s)	1641
BsrGI	TGTACA	2 cut(s)	83, 2070
BsrI	ACTGG	8 cut(s)	16, 45, 477, 589, 852, 917, 1435, 1543
BssECI	CCNNGG	4 cut(s)	18, 757, 819, 1056
BssT1I	CCWWGG	2 cut(s)	757, 1056
Bst2UI	CCWGG	1 cut(s)	1982
Bst4CI	ACNGT	6 cut(s)	88, 202, 281, 881, 1999, 2018
Bst6I	CTCTTC	1 cut(s)	799
BstAFI	CTTAAG	1 cut(s)	1801
BstAPI	GCANNNNNTGC	1 cut(s)	1829
BstAUI	TGTACA	2 cut(s)	83, 2070
BstBAI	YACGTR	1 cut(s)	1490
BstC8I	GCNNGC	5 cut(s)	405, 633, 1179, 1425, 1455
BstDEI	CTNAG	6 cut(s)	615, 867, 1102, 1323, 1725, 2004
BstDSI	CCRYGG	1 cut(s)	757
BstF5I	GGATG	7 cut(s)	997, 1141, 1699, 1705, 1790, 1855, 1914
BstFNI	CGCG	1 cut(s)	237
BstH2I	RGCGCY	1 cut(s)	1066
BstHHI	GCGC	2 cut(s)	631, 1065
BstMAI	GTCTC	6 cut(s)	1523, 1538, 1658, 1721, 1726, 1778
BstMWI	GCNNNNNNNGC	3 cut(s)	409, 1074, 1829
BstNI	CCWGG	1 cut(s)	1982
BstNSI	RCATGY	1 cut(s)	1427
BstSCI	CCNGG	1 cut(s)	1980
BstSFI	CTRYAG	4 cut(s)	74, 1821, 1891, 1957
BstUI	CGCG	1 cut(s)	237
BstV1I	GCAGC	4 cut(s)	88, 1052, 1835, 1914
BstV2I	GAAGAC	3 cut(s)	1101, 1223, 1401
BstX2I	RGATCY	6 cut(s)	438, 1141, 1312, 1639, 1660, 1977
BstYI	RGATCY	6 cut(s)	438, 1141, 1312, 1639, 1660, 1977
Bsu36I	CCTNAGG	1 cut(s)	2004
BsuI	GTATCC	1 cut(s)	1220
BsuRI	GGCC	3 cut(s)	1119, 1157, 1681
BtgI	CCRYGG	1 cut(s)	757
BtsCI	GGATG	7 cut(s)	997, 1141, 1699, 1705, 1790, 1855, 1914
BtsI	GCAGTG	2 cut(s)	71, 347
BtsIMutI	CAGTG	5 cut(s)	9, 71, 207, 347, 1536
BveI	ACCTGC	2 cut(s)	760, 1873
Cac8I	GCNNGC	5 cut(s)	405, 633, 1179, 1425, 1455
CaiI	CAGNNNCTG	2 cut(s)	923, 2003
CciI	TCATGA	1 cut(s)	949
CfoI	GCGC	2 cut(s)	631, 1065
Cfr13I	GGNCC	3 cut(s)	170, 326, 749
CseI	GACGC	2 cut(s)	713, 975
Csp6I	GTAC	6 cut(s)	84, 371, 882, 890, 1818, 2071
CspCI	CAANNNNNGTGG	4 cut(s)	471, 506, 1671, 1706
CviAII	CATG	8 cut(s)	758, 950, 1031, 1071, 1186, 1289, 1424, 1675
CviQI	GTAC	6 cut(s)	84, 371, 882, 890, 1818, 2071
DdeI	CTNAG	6 cut(s)	615, 867, 1102, 1323, 1725, 2004
EaeI	YGGCCR	1 cut(s)	1155
Eam1104I	CTCTTC	1 cut(s)	799
EarI	CTCTTC	1 cut(s)	799
Ecl136II	GAGCTC	1 cut(s)	641
Eco130I	CCWWGG	2 cut(s)	757, 1056
Eco24I	GRGCYC	1 cut(s)	643
Eco32I	GATATC	1 cut(s)	146
Eco47I	GGWCC	3 cut(s)	170, 326, 749
Eco47III	AGCGCT	1 cut(s)	1064
Eco53kI	GAGCTC	1 cut(s)	641
Eco72I	CACGTG	1 cut(s)	1490
Eco81I	CCTNAGG	1 cut(s)	2004
Eco88I	CYCGRG	1 cut(s)	53
EcoICRI	GAGCTC	1 cut(s)	641
EcoO109I	RGGNCCY	1 cut(s)	326
EcoRII	CCWGG	1 cut(s)	1980
EcoRV	GATATC	1 cut(s)	146
EcoT14I	CCWWGG	2 cut(s)	757, 1056
EcoT38I	GRGCYC	1 cut(s)	643
ErhI	CCWWGG	2 cut(s)	757, 1056
Esp3I	CGTCTC	2 cut(s)	1658, 1721
FaeI	CATG	8 cut(s)	761, 953, 1034, 1074, 1189, 1292, 1427, 1678
FaqI	GGGAC	3 cut(s)	837, 1415, 2008
FatI	CATG	8 cut(s)	757, 949, 1030, 1070, 1185, 1288, 1423, 1674
FbaI	TGATCA	2 cut(s)	991, 1033
Fnu4HI	GCNGC	5 cut(s)	77, 1066, 1481, 1824, 1928
FokI	GGATG	7 cut(s)	984, 1148, 1692, 1706, 1777, 1862, 1901
FriOI	GRGCYC	1 cut(s)	643
Fsp4HI	GCNGC	5 cut(s)	77, 1066, 1481, 1824, 1928
FspBI	CTAG	3 cut(s)	435, 453, 1755
GlaI	GCGC	2 cut(s)	630, 1064
GluI	GCNGC	5 cut(s)	77, 1066, 1481, 1824, 1928
GsaI	CCCAGC	2 cut(s)	1423, 1798
GsuI	CTGGAG	1 cut(s)	1738
HaeII	RGCGCY	1 cut(s)	1066
HaeIII	GGCC	3 cut(s)	1119, 1157, 1681
HgaI	GACGC	2 cut(s)	713, 975
HhaI	GCGC	2 cut(s)	631, 1065
Hin1II	CATG	8 cut(s)	761, 953, 1034, 1074, 1189, 1292, 1427, 1678
Hin6I	GCGC	2 cut(s)	629, 1063
HinP1I	GCGC	2 cut(s)	629, 1063
HindIII	AAGCTT	1 cut(s)	1364
HinfI	GANTC	8 cut(s)	260, 431, 497, 740, 970, 976, 1106, 2034
HphI	GGTGA	3 cut(s)	535, 749, 1227
Hpy166II	GTNNAC	4 cut(s)	430, 470, 599, 1547
Hpy8I	GTNNAC	4 cut(s)	430, 470, 599, 1547
HpyAV	CCTTC	5 cut(s)	208, 317, 383, 1242, 1403
HpyCH4III	ACNGT	6 cut(s)	88, 202, 281, 881, 1999, 2018
HpyCH4IV	ACGT	2 cut(s)	826, 1489
HpyF10VI	GCNNNNNNNGC	3 cut(s)	409, 1074, 1829
HpyF3I	CTNAG	6 cut(s)	615, 867, 1102, 1323, 1725, 2004
HpySE526I	ACGT	2 cut(s)	826, 1489
Hsp92II	CATG	8 cut(s)	761, 953, 1034, 1074, 1189, 1292, 1427, 1678
HspAI	GCGC	2 cut(s)	629, 1063
KpnI	GGTACC	1 cut(s)	885
Ksp22I	TGATCA	2 cut(s)	991, 1033
LmnI	GCTCC	4 cut(s)	638, 646, 1027, 1060
Lsp1109I	GCAGC	4 cut(s)	88, 1052, 1835, 1914
LweI	GCATC	6 cut(s)	121, 1006, 1711, 1741, 1799, 1923
MaeI	CTAG	3 cut(s)	435, 453, 1755
MaeII	ACGT	2 cut(s)	826, 1489
MaeIII	GTNAC	5 cut(s)	812, 840, 875, 1898, 1919
MfeI	CAATTG	1 cut(s)	1172
MflI	RGATCY	6 cut(s)	438, 1141, 1312, 1639, 1660, 1977
MhlI	GDGCHC	1 cut(s)	643
MlyI	GAGTC	2 cut(s)	425, 1115
MmeI	TCCRAC	1 cut(s)	6
MroXI	GAANNNNTTC	3 cut(s)	686, 1467, 2097
MseI	TTAA	7 cut(s)	927, 1079, 1247, 1253, 1802, 1949, 2048
MslI	CAYNNNNRTG	4 cut(s)	384, 951, 1287, 1554
MspA1I	CMGCKG	2 cut(s)	1480, 1688
MspCI	CTTAAG	1 cut(s)	1801
MspR9I	CCNGG	1 cut(s)	1982
MunI	CAATTG	1 cut(s)	1172
Mva1269I	GAATGC	2 cut(s)	1167, 1868
MvaI	CCWGG	1 cut(s)	1982
MvnI	CGCG	1 cut(s)	237
MwoI	GCNNNNNNNGC	3 cut(s)	409, 1074, 1829
NcoI	CCATGG	1 cut(s)	757
NlaIII	CATG	8 cut(s)	761, 953, 1034, 1074, 1189, 1292, 1427, 1678
NlaIV	GGNNCC	6 cut(s)	6, 16, 883, 1143, 1662, 1863
NmeAIII	GCCGAG	2 cut(s)	43, 1183
NmuCI	GTSAC	2 cut(s)	812, 1919
NspI	RCATGY	1 cut(s)	1427
PaeI	GCATGC	1 cut(s)	1427
PaeR7I	CTCGAG	1 cut(s)	53
PagI	TCATGA	1 cut(s)	949
PcsI	WCGNNNNNNNCGW	3 cut(s)	937, 1736, 1894
PctI	GAATGC	2 cut(s)	1167, 1868
PdmI	GAANNNNTTC	3 cut(s)	686, 1467, 2097
PfeI	GAWTC	6 cut(s)	260, 497, 740, 970, 976, 2034
PfoI	TCCNGGA	1 cut(s)	1980
PkrI	GCNGC	5 cut(s)	78, 1067, 1482, 1825, 1929
PleI	GAGTC	2 cut(s)	425, 1114
PmaCI	CACGTG	1 cut(s)	1490
PmlI	CACGTG	1 cut(s)	1490
PpsI	GAGTC	2 cut(s)	425, 1114
Ppu21I	YACGTR	1 cut(s)	1490
PpuMI	RGGWCCY	1 cut(s)	326
PshBI	ATTAAT	2 cut(s)	1253, 2048
PsiI	TTATAA	1 cut(s)	2061
Psp124BI	GAGCTC	1 cut(s)	643
Psp5II	RGGWCCY	1 cut(s)	326
Psp6I	CCWGG	1 cut(s)	1980
PspCI	CACGTG	1 cut(s)	1490
PspFI	CCCAGC	2 cut(s)	1419, 1794
PspGI	CCWGG	1 cut(s)	1980
PspN4I	GGNNCC	6 cut(s)	6, 16, 883, 1143, 1662, 1863
PspPI	GGNCC	3 cut(s)	170, 326, 749
PspPPI	RGGWCCY	1 cut(s)	326
PstI	CTGCAG	2 cut(s)	78, 1825
PstNI	CAGNNNCTG	2 cut(s)	923, 2003
PsuI	RGATCY	6 cut(s)	438, 1141, 1312, 1639, 1660, 1977
PvuII	CAGCTG	1 cut(s)	1688
RsaI	GTAC	6 cut(s)	85, 372, 883, 891, 1819, 2072
RsaNI	GTAC	6 cut(s)	84, 371, 882, 890, 1818, 2071
RseI	CAYNNNNRTG	4 cut(s)	384, 951, 1287, 1554
SacI	GAGCTC	1 cut(s)	643
SaqAI	TTAA	7 cut(s)	927, 1079, 1247, 1253, 1802, 1949, 2048
SatI	GCNGC	5 cut(s)	77, 1066, 1481, 1824, 1928
Sau96I	GGNCC	3 cut(s)	170, 326, 749
ScaI	AGTACT	1 cut(s)	1819
SchI	GAGTC	2 cut(s)	425, 1115
ScrFI	CCNGG	1 cut(s)	1982
SduI	GDGCHC	1 cut(s)	643
SfaNI	GCATC	6 cut(s)	121, 1006, 1711, 1741, 1799, 1923
SfcI	CTRYAG	4 cut(s)	74, 1821, 1891, 1957
Sfr274I	CTCGAG	1 cut(s)	53
SinI	GGWCC	3 cut(s)	170, 326, 749
SlaI	CTCGAG	1 cut(s)	53
SmiMI	CAYNNNNRTG	4 cut(s)	384, 951, 1287, 1554
SmlI	CTYRAG	4 cut(s)	53, 893, 1113, 1801
SmoI	CTYRAG	4 cut(s)	53, 893, 1113, 1801
SphI	GCATGC	1 cut(s)	1427
SsiI	CCGC	1 cut(s)	1480
SspI	AATATT	1 cut(s)	1006
SspMI	CTAG	3 cut(s)	435, 453, 1755
SstI	GAGCTC	1 cut(s)	643
StyD4I	CCNGG	1 cut(s)	1980
StyI	CCWWGG	2 cut(s)	757, 1056
TaaI	ACNGT	6 cut(s)	88, 202, 281, 881, 1999, 2018
TaiI	ACGT	2 cut(s)	829, 1492
TaqI	TCGA	7 cut(s)	54, 142, 661, 1569, 2013, 2032, 2052
TatI	WGTACW	4 cut(s)	83, 889, 1817, 2070
TauI	GCSGC	1 cut(s)	1483
TfiI	GAWTC	6 cut(s)	260, 497, 740, 970, 976, 2034
Tru1I	TTAA	7 cut(s)	927, 1079, 1247, 1253, 1802, 1949, 2048
Tru9I	TTAA	7 cut(s)	927, 1079, 1247, 1253, 1802, 1949, 2048
TscAI	CASTG	5 cut(s)	16, 78, 207, 354, 1543
TseFI	GTSAC	2 cut(s)	812, 1919
TseI	GCWGC	4 cut(s)	76, 1065, 1823, 1927
Tsp45I	GTSAC	2 cut(s)	812, 1919
TspDTI	ATGAA	7 cut(s)	402, 738, 786, 873, 1305, 1797, 1866
TspGWI	ACGGA	2 cut(s)	1001, 1907
TspRI	CASTG	5 cut(s)	16, 78, 207, 354, 1543
Vha464I	CTTAAG	1 cut(s)	1801
VpaK11BI	GGWCC	3 cut(s)	170, 326, 749
VspI	ATTAAT	2 cut(s)	1253, 2048
XapI	RAATTY	3 cut(s)	422, 685, 2093
XceI	RCATGY	1 cut(s)	1427
XcmI	CCANNNNNNNNNTGG	2 cut(s)	487, 1427
XhoI	CTCGAG	1 cut(s)	53
XmnI	GAANNNNTTC	3 cut(s)	686, 1467, 2097
XspI	CTAG	3 cut(s)	435, 453, 1755
ZrmI	AGTACT	1 cut(s)	1819

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.