Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0006268

Physical Location & Seq

Genomic Coordinates Forward (+)

5197223 .. 5200991

Gene Structure 3769 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1040300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 492 bp

ATGGCTCCACCACGATCTCTGATCCTTCACCGGCTCACGCCCTACTTTGCAGCTCGAATCAGGCAGAACCAGAGGCTCCTATCCTCTTCTTTCTTCTATTCCTCTTCCTCTTCCGCCGCAGAAGAAGCCTCCTCTTCTTCTCCATCTCCGTCCGTCGACCTCGATTCCGTTCACATGACTGATACCTGTGTTCAAAGAATGAAGCAGCTGCAAGCCAGTGAAGCGGAGGGAAAGGTGCTACGTTTGAGTGTAGAAACCGGTGGATGTTCTGGGTTCCAATATGTTTTTGATCTGGATGACAAAAGCCATCCGGATGACAGAGTATTTGAGAAAGGAGGAGTAAAGTTGGTGGTCGACAATATTTCATATGATTTTGTGAAAGGAGCAACTGTTGATTATGTTGAGGAGCTGATTCGTTCTGCTTTCCAAGTGACTACAAATCCAAGTGCGGTTGGGGGGTGCAGTTGTAAAAGTTCTTTCATGGTGAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005506 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016043 GO:0016226 GO:0019538 GO:0022607 GO:0031163 GO:0043167 GO:0043169 GO:0043170 GO:0044085 GO:0044237 GO:0044238 GO:0046872 GO:0046914 GO:0048037 GO:0051186 GO:0051536 GO:0051537 GO:0051539 GO:0051540 GO:0051604 GO:0071704 GO:0071840 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Fe-S_biosyn

Protein Analysis

164

Amino Acids

17.82

Weight (kDa)

5.9

Isoelectric Point (pI)

49.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Fe-S_biosyn	PF01521	57 - 157	9.8e-15	Iron-sulphur cluster biosynthesis

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0006268)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G03905 AT5G03905
fragaria_vesca	FvH4_6g18690 FvH4_6g18690
malus_domestica	MD06G1040300.v1.1 MD16G1269100.v1.1
prunus_persica	Prupe.5G046500_v2.0.a1 Prupe.5G046500_v2.0.a1 Prupe.5G046500_v2.0.a1 Prupe.5G046500_v2.0.a1
pyrus_communis	pycom06g03360 pycom16g23810
rosa_chinensis	RchiOBHm_Chr3g0473661
rosa_laevigata	RLG00000023996
rosa_multiflora	Rmu_co8353519.1_g000001 Rmu_sc0003069.1_g000005 Rmu_sc0004730.1_g000005 Rmu_sc0004984.1_g000003 Rmu_sc0005978.1_g000002
rosa_roxburghii	Rroxscaffold_6G00407970
rosa_rugosa	Rorug03G0133700
rosa_samantha	Rh3AG185300 Rh3BG213300 Rh3CG210000 Rh3DG209200 Rh5AG365500
rosa_wichuraiana	Rw0G008900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	2 cut(s)	156, 354
AccIII	TCCGGA	1 cut(s)	310
AciI	CCGC	4 cut(s)	114, 117, 224, 449
AclWI	GGATC	1 cut(s)	16
AgeI	ACCGGT	1 cut(s)	257
AgsI	TTSAA	1 cut(s)	194
AluBI	AGCT	3 cut(s)	53, 208, 409
AluI	AGCT	3 cut(s)	53, 208, 409
AlwI	GGATC	1 cut(s)	16
Aor13HI	TCCGGA	1 cut(s)	310
ApeKI	GCWGC	3 cut(s)	50, 205, 208
ArsI	GACNNNNNNTTYG	2 cut(s)	308, 340
AsiGI	ACCGGT	1 cut(s)	257
AsuHPI	GGTGA	1 cut(s)	20
BaeI	ACNNNNGTAYC	2 cut(s)	174, 207
BbvI	GCAGC	3 cut(s)	62, 195, 217
BccI	CCATC	2 cut(s)	151, 315
BisI	GCNGC	4 cut(s)	51, 117, 206, 209
BlsI	GCNGC	4 cut(s)	52, 118, 207, 210
BmiI	GGNNCC	3 cut(s)	6, 77, 275
BsaWI	WCCGGW	2 cut(s)	257, 310
BsaXI	ACNNNNNCTCC	2 cut(s)	375, 405
Bse118I	RCCGGY	2 cut(s)	30, 257
Bse1I	ACTGG	1 cut(s)	216
BseAI	TCCGGA	1 cut(s)	310
BseGI	GGATG	4 cut(s)	269, 301, 307, 319
BseNI	ACTGG	1 cut(s)	216
BseRI	GAGGAG	3 cut(s)	121, 351, 419
BseXI	GCAGC	3 cut(s)	62, 195, 217
BsgI	GTGCAG	1 cut(s)	481
BshTI	ACCGGT	1 cut(s)	257
BsiSI	CCGG	3 cut(s)	31, 258, 311
Bsp13I	TCCGGA	1 cut(s)	310
Bsp143I	GATC	3 cut(s)	14, 21, 289
BspACI	CCGC	4 cut(s)	114, 117, 224, 449
BspEI	TCCGGA	1 cut(s)	310
BspLI	GGNNCC	3 cut(s)	6, 77, 275
BspPI	GGATC	1 cut(s)	16
BsrFI	RCCGGY	2 cut(s)	30, 257
BsrI	ACTGG	1 cut(s)	216
BssAI	RCCGGY	2 cut(s)	30, 257
BssMI	GATC	3 cut(s)	14, 21, 289
Bst4CI	ACNGT	1 cut(s)	391
Bst6I	CTCTTC	4 cut(s)	91, 109, 115, 139
BstC8I	GCNNGC	1 cut(s)	213
BstF5I	GGATG	4 cut(s)	269, 301, 307, 319
BstKTI	GATC	3 cut(s)	17, 24, 292
BstMBI	GATC	3 cut(s)	14, 21, 289
BstMWI	GCNNNNNNNGC	2 cut(s)	125, 221
BstV1I	GCAGC	3 cut(s)	62, 195, 217
BtsCI	GGATG	4 cut(s)	269, 301, 307, 319
BtsIMutI	CAGTG	1 cut(s)	223
Cac8I	GCNNGC	1 cut(s)	213
Cfr10I	RCCGGY	2 cut(s)	30, 257
CspAI	ACCGGT	1 cut(s)	257
CviAII	CATG	2 cut(s)	175, 481
CviJI	RGCY	9 cut(s)	5, 34, 53, 76, 128, 208, 215, 306, 409
CviKI_1	RGCY	9 cut(s)	5, 34, 53, 76, 128, 208, 215, 306, 409
DpnI	GATC	3 cut(s)	16, 23, 291
DpnII	GATC	3 cut(s)	14, 21, 289
Eam1104I	CTCTTC	4 cut(s)	91, 109, 115, 139
EarI	CTCTTC	4 cut(s)	91, 109, 115, 139
EciI	GGCGGA	1 cut(s)	103
FaeI	CATG	2 cut(s)	178, 484
FaiI	YATR	6 cut(s)	176, 282, 367, 369, 399, 482
FatI	CATG	2 cut(s)	174, 480
FauNDI	CATATG	1 cut(s)	367
FblI	GTMKAC	2 cut(s)	156, 354
Fnu4HI	GCNGC	4 cut(s)	51, 117, 206, 209
FokI	GGATG	4 cut(s)	276, 294, 308, 326
Fsp4HI	GCNGC	4 cut(s)	51, 117, 206, 209
GluI	GCNGC	4 cut(s)	51, 117, 206, 209
HapII	CCGG	3 cut(s)	31, 258, 311
Hin1II	CATG	2 cut(s)	178, 484
HincII	GTYRAC	2 cut(s)	157, 355
HindII	GTYRAC	2 cut(s)	157, 355
HinfI	GANTC	3 cut(s)	57, 164, 412
HpaII	CCGG	3 cut(s)	31, 258, 311
HphI	GGTGA	1 cut(s)	20
Hpy166II	GTNNAC	3 cut(s)	157, 172, 355
Hpy188I	TCNGA	1 cut(s)	21
Hpy188III	TCNNGA	2 cut(s)	293, 311
Hpy8I	GTNNAC	3 cut(s)	157, 172, 355
Hpy99I	CGWCG	1 cut(s)	158
HpyAV	CCTTC	1 cut(s)	35
HpyCH4III	ACNGT	1 cut(s)	391
HpyCH4IV	ACGT	1 cut(s)	241
HpyCH4V	TGCA	3 cut(s)	50, 211, 462
HpyF10VI	GCNNNNNNNGC	2 cut(s)	125, 221
HpySE526I	ACGT	1 cut(s)	241
Hsp92II	CATG	2 cut(s)	178, 484
Kpn2I	TCCGGA	1 cut(s)	310
Kzo9I	GATC	3 cut(s)	14, 21, 289
LmnI	GCTCC	4 cut(s)	10, 81, 383, 406
LpnPI	CCDG	9 cut(s)	44, 46, 83, 199, 229, 255, 271, 278, 324
Lsp1109I	GCAGC	3 cut(s)	62, 195, 217
MaeII	ACGT	1 cut(s)	241
MaeIII	GTNAC	1 cut(s)	430
MalI	GATC	3 cut(s)	16, 23, 291
MboI	GATC	3 cut(s)	14, 21, 289
MboII	GAAGA	7 cut(s)	78, 85, 96, 102, 126, 129, 134
MroI	TCCGGA	1 cut(s)	310
MslI	CAYNNNNRTG	1 cut(s)	312
MspA1I	CMGCKG	1 cut(s)	208
MspI	CCGG	3 cut(s)	31, 258, 311
MwoI	GCNNNNNNNGC	2 cut(s)	125, 221
NdeI	CATATG	1 cut(s)	367
NdeII	GATC	3 cut(s)	14, 21, 289
NlaIII	CATG	2 cut(s)	178, 484
NlaIV	GGNNCC	3 cut(s)	6, 77, 275
NmuCI	GTSAC	1 cut(s)	430
PfeI	GAWTC	3 cut(s)	57, 164, 412
PinAI	ACCGGT	1 cut(s)	257
PkrI	GCNGC	4 cut(s)	52, 118, 207, 210
PspN4I	GGNNCC	3 cut(s)	6, 77, 275
PvuII	CAGCTG	1 cut(s)	208
RseI	CAYNNNNRTG	1 cut(s)	312
SalI	GTCGAC	2 cut(s)	155, 353
SatI	GCNGC	4 cut(s)	51, 117, 206, 209
Sau3AI	GATC	3 cut(s)	14, 21, 289
SetI	ASST	7 cut(s)	55, 162, 188, 210, 237, 244, 411
SmiMI	CAYNNNNRTG	1 cut(s)	312
SsiI	CCGC	4 cut(s)	114, 117, 224, 449
SspI	AATATT	1 cut(s)	361
TaaI	ACNGT	1 cut(s)	391
TaiI	ACGT	1 cut(s)	244
TaqI	TCGA	4 cut(s)	55, 156, 162, 354
TauI	GCSGC	1 cut(s)	119
TfiI	GAWTC	3 cut(s)	57, 164, 412
TscAI	CASTG	1 cut(s)	223
TseFI	GTSAC	1 cut(s)	430
TseI	GCWGC	3 cut(s)	50, 205, 208
Tsp45I	GTSAC	1 cut(s)	430
TspDTI	ATGAA	3 cut(s)	215, 354, 469
TspGWI	ACGGA	3 cut(s)	138, 142, 157
TspRI	CASTG	1 cut(s)	223
XmiI	GTMKAC	2 cut(s)	156, 354

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.