RLG00000023996

Iron-sulfur assembly protein IscA-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0006268
Physical Location & Seq
Forward (+)
30756952 .. 30759396
2445 bp
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UTR
Exon/CDS
Intron
RLM00000023996

Sequence Viewer

Length: 480 bp
ATGGCTCCGAGGACGTTGATTCTTCACCGGTTGACGGCGCACTTTGCCGGTCGAATCAGGCAGAACCAGAGGCTTCTCAGCTCTCATTTTTATTCCTCTACGGCGTTGGAAGAAGCCCCTTCTTCTTCTTCCTCGCCGAACTTGGATGGCGTTTATATGACGGACACATGTGTTCAGAAAATGAAACAACTGCAAGCCAGTGAAGCACAGGAAAAGCTGCTCCGCTTGAGTGTAGAAACAGGTGGATGTTCTGGGTTCCAATATGTTTTTGATCTGGTGGATGACAAGACCAATCCAGATGACAGGGTGTTCGAGAAAGAAGGAGTAAAATTGGTAGTCGACAATATTTCATATGATTTTTTGAAAGGGGCAACCGTTGATTATGTTGAGGAGCTGATCCGTTCTGCTTTCCAAGTGACAGAAAATCCAAGTGCAGTCCAGGGTTGCAGTTGTAAAAGTTCTTTCATGGTGAAATTATAG

Protein Analysis

160

Amino Acids

17.71

Weight (kDa)

5.61

Isoelectric Point (pI)

42.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fe-S_biosyn PF01521 52 - 152 3.1e-16 Iron-sulphur cluster biosynthesis
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 339
AciI CCGC 1 cut(s) 223
AclWI GGATC 1 cut(s) 391
AfiI CCNNNNNNNGG 1 cut(s) 34
AflIII ACRYGT 1 cut(s) 167
AgeI ACCGGT 1 cut(s) 27
AgsI TTSAA 1 cut(s) 364
AjnI CCWGG 1 cut(s) 438
AluBI AGCT 3 cut(s) 81, 217, 394
AluI AGCT 3 cut(s) 81, 217, 394
AlwI GGATC 1 cut(s) 391
ApeKI GCWGC 1 cut(s) 217
ArsI GACNNNNNNTTYG 2 cut(s) 293, 325
AsiGI ACCGGT 1 cut(s) 27
AspLEI GCGC 1 cut(s) 40
AsuHPI GGTGA 1 cut(s) 17
BbvI GCAGC 1 cut(s) 204
BccI CCATC 1 cut(s) 140
BceAI ACGGC 2 cut(s) 51, 117
BciT130I CCWGG 1 cut(s) 440
BisI GCNGC 1 cut(s) 218
BlsI GCNGC 1 cut(s) 219
Bme1390I CCNGG 1 cut(s) 440
BmiI GGNNCC 2 cut(s) 6, 257
BmrFI CCNGG 1 cut(s) 440
BpuEI CTTGAG 1 cut(s) 247
BsaJI CCNNGG 2 cut(s) 8, 439
BsaWI WCCGGW 1 cut(s) 27
Bsc4I CCNNNNNNNGG 1 cut(s) 34
Bse118I RCCGGY 2 cut(s) 27, 47
Bse1I ACTGG 1 cut(s) 198
BseBI CCWGG 1 cut(s) 440
BseDI CCNNGG 2 cut(s) 8, 439
BseGI GGATG 3 cut(s) 151, 251, 286
BseLI CCNNNNNNNGG 1 cut(s) 34
BseMII CTCAG 1 cut(s) 91
BseNI ACTGG 1 cut(s) 198
BseRI GAGGAG 1 cut(s) 404
BseXI GCAGC 1 cut(s) 204
BsgI GTGCAG 1 cut(s) 453
Bsh1285I CGRYCG 1 cut(s) 52
BshTI ACCGGT 1 cut(s) 27
BsiEI CGRYCG 1 cut(s) 52
BsiSI CCGG 2 cut(s) 28, 48
BslI CCNNNNNNNGG 1 cut(s) 34
Bsp143I GATC 2 cut(s) 271, 396
BspACI CCGC 1 cut(s) 223
BspCNI CTCAG 1 cut(s) 90
BspLI GGNNCC 2 cut(s) 6, 257
BspPI GGATC 1 cut(s) 391
BsrFI RCCGGY 2 cut(s) 27, 47
BsrI ACTGG 1 cut(s) 198
BssAI RCCGGY 2 cut(s) 27, 47
BssECI CCNNGG 2 cut(s) 8, 439
BssMI GATC 2 cut(s) 271, 396
Bst2UI CCWGG 1 cut(s) 440
Bst4CI ACNGT 1 cut(s) 376
BstC8I GCNNGC 1 cut(s) 195
BstDEI CTNAG 1 cut(s) 77
BstF5I GGATG 3 cut(s) 151, 251, 286
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 2 cut(s) 274, 399
BstMBI GATC 2 cut(s) 271, 396
BstMCI CGRYCG 1 cut(s) 52
BstMWI GCNNNNNNNGC 2 cut(s) 44, 203
BstNI CCWGG 1 cut(s) 440
BstNSI RCATGY 1 cut(s) 171
BstSCI CCNGG 1 cut(s) 438
BstV1I GCAGC 1 cut(s) 204
BtsCI GGATG 3 cut(s) 151, 251, 286
BtsIMutI CAGTG 1 cut(s) 205
Cac8I GCNNGC 1 cut(s) 195
CfoI GCGC 1 cut(s) 40
Cfr10I RCCGGY 2 cut(s) 27, 47
CspAI ACCGGT 1 cut(s) 27
CviAII CATG 2 cut(s) 168, 466
CviJI RGCY 7 cut(s) 5, 73, 81, 116, 197, 217, 394
CviKI_1 RGCY 7 cut(s) 5, 73, 81, 116, 197, 217, 394
DdeI CTNAG 1 cut(s) 77
DpnI GATC 2 cut(s) 273, 398
DpnII GATC 2 cut(s) 271, 396
EcoRII CCWGG 1 cut(s) 438
FaeI CATG 2 cut(s) 171, 469
FaiI YATR 9 cut(s) 156, 158, 169, 264, 352, 354, 384, 467, 478
FatI CATG 2 cut(s) 167, 465
FauNDI CATATG 1 cut(s) 352
FblI GTMKAC 1 cut(s) 339
Fnu4HI GCNGC 1 cut(s) 218
FokI GGATG 3 cut(s) 158, 258, 293
Fsp4HI GCNGC 1 cut(s) 218
GlaI GCGC 1 cut(s) 39
GluI GCNGC 1 cut(s) 218
HapII CCGG 2 cut(s) 28, 48
HhaI GCGC 1 cut(s) 40
Hin1II CATG 2 cut(s) 171, 469
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HincII GTYRAC 2 cut(s) 33, 340
HindII GTYRAC 2 cut(s) 33, 340
HinfI GANTC 2 cut(s) 19, 54
HpaII CCGG 2 cut(s) 28, 48
HphI GGTGA 1 cut(s) 17
Hpy166II GTNNAC 2 cut(s) 33, 340
Hpy188I TCNGA 2 cut(s) 9, 177
Hpy188III TCNNGA 2 cut(s) 296, 313
Hpy8I GTNNAC 2 cut(s) 33, 340
HpyAV CCTTC 2 cut(s) 129, 314
HpyCH4III ACNGT 1 cut(s) 376
HpyCH4IV ACGT 1 cut(s) 14
HpyCH4V TGCA 3 cut(s) 193, 434, 447
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 203
HpyF3I CTNAG 1 cut(s) 77
HpySE526I ACGT 1 cut(s) 14
Hsp92II CATG 2 cut(s) 171, 469
HspAI GCGC 1 cut(s) 38
Kzo9I GATC 2 cut(s) 271, 396
LmnI GCTCC 3 cut(s) 10, 225, 391
Lsp1109I GCAGC 1 cut(s) 204
MaeII ACGT 1 cut(s) 14
MaeIII GTNAC 1 cut(s) 415
MalI GATC 2 cut(s) 273, 398
MboI GATC 2 cut(s) 271, 396
MboII GAAGA 5 cut(s) 14, 114, 117, 120, 122
MluCI AATT 2 cut(s) 329, 473
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 5 cut(s) 3, 63, 106, 142, 382
MspI CCGG 2 cut(s) 28, 48
MspR9I CCNGG 1 cut(s) 440
MvaI CCWGG 1 cut(s) 440
MwoI GCNNNNNNNGC 2 cut(s) 44, 203
NdeI CATATG 1 cut(s) 352
NdeII GATC 2 cut(s) 271, 396
NlaIII CATG 2 cut(s) 171, 469
NlaIV GGNNCC 2 cut(s) 6, 257
NmuCI GTSAC 1 cut(s) 415
NspI RCATGY 1 cut(s) 171
PciI ACATGT 1 cut(s) 167
PfeI GAWTC 2 cut(s) 19, 54
PinAI ACCGGT 1 cut(s) 27
PkrI GCNGC 1 cut(s) 219
PscI ACATGT 1 cut(s) 167
Psp6I CCWGG 1 cut(s) 438
PspGI CCWGG 1 cut(s) 438
PspN4I GGNNCC 2 cut(s) 6, 257
SalI GTCGAC 1 cut(s) 338
SatI GCNGC 1 cut(s) 218
Sau3AI GATC 2 cut(s) 271, 396
ScrFI CCNGG 1 cut(s) 440
SetI ASST 5 cut(s) 17, 83, 219, 244, 396
SmlI CTYRAG 1 cut(s) 226
SmoI CTYRAG 1 cut(s) 226
Sse9I AATT 2 cut(s) 329, 473
SsiI CCGC 1 cut(s) 223
SspI AATATT 1 cut(s) 346
StyD4I CCNGG 1 cut(s) 438
TaaI ACNGT 1 cut(s) 376
TaiI ACGT 1 cut(s) 17
TaqI TCGA 3 cut(s) 52, 312, 339
TasI AATT 2 cut(s) 329, 473
TfiI GAWTC 2 cut(s) 19, 54
TscAI CASTG 1 cut(s) 205
TseFI GTSAC 1 cut(s) 415
TseI GCWGC 1 cut(s) 217
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 3 cut(s) 197, 339, 454
TspGWI ACGGA 2 cut(s) 176, 389
TspRI CASTG 1 cut(s) 205
XceI RCATGY 1 cut(s) 171
XmiI GTMKAC 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.