RchiOBHm_Chr3g0473661

Iron-sulfur assembly protein IscA-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0006268
Physical Location & Seq
Reverse (-)
19458572 .. 19461605
3034 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43932

Sequence Viewer

Length: 489 bp
ATGGCTCCGAGGACGTTGATTCTTCACCGGTTGACGGCGCACTTTGCCGGTCGAATCAGGCAGAACCAGAGGCTTCTCAGCTCTCATTTTTATTCCTCCACGGCGTTGGAAGAAGCCCCTTCCTCTTCTTCTTCTTCCTCCTCGCCGAACTTGGACGGCGTTTACATGACGGACACTTGTGTTCAGAAAATGAAACAACTGCAAGCCAGTGAAGCACAGGAAAAGCTGCTCCGCTTGAGTGTAGAAACAGGTGGATGTTCTGGGTTCCAATATGTTTTTGATCTGGTGGATGACAAAACCAATCCAGATGACAGGGTGTTCGAGAAAGAAGGAGTAAAATTGGTAGTCGACAATATTTCATATGATTTTTTGAAAGGGGCAACCGTTGATTATGTTGAGGAGCTGATCCGTTCTGCTTTCCAAGTGACAGAAAATCCAAGTGCAGTGCAGGGTTGCAGTTGTAAAAGTTCTTTCATGGTGAAATTATAG

Protein Analysis

162

Amino Acids

17.97

Weight (kDa)

5.61

Isoelectric Point (pI)

45.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fe-S_biosyn PF01521 55 - 155 3.3e-16 Iron-sulphur cluster biosynthesis
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 348
AciI CCGC 1 cut(s) 232
AclWI GGATC 1 cut(s) 400
AfiI CCNNNNNNNGG 1 cut(s) 34
AgeI ACCGGT 1 cut(s) 27
AgsI TTSAA 1 cut(s) 373
AluBI AGCT 3 cut(s) 81, 226, 403
AluI AGCT 3 cut(s) 81, 226, 403
AlwI GGATC 1 cut(s) 400
ApeKI GCWGC 1 cut(s) 226
ArsI GACNNNNNNTTYG 2 cut(s) 302, 334
AsiGI ACCGGT 1 cut(s) 27
AspLEI GCGC 1 cut(s) 40
AsuHPI GGTGA 1 cut(s) 17
BbvI GCAGC 1 cut(s) 213
BceAI ACGGC 3 cut(s) 51, 117, 172
BisI GCNGC 1 cut(s) 227
BlsI GCNGC 1 cut(s) 228
BmiI GGNNCC 2 cut(s) 6, 266
BpuEI CTTGAG 1 cut(s) 256
BsaJI CCNNGG 2 cut(s) 8, 99
BsaWI WCCGGW 1 cut(s) 27
Bsc4I CCNNNNNNNGG 1 cut(s) 34
Bse118I RCCGGY 2 cut(s) 27, 47
Bse1I ACTGG 1 cut(s) 207
BseDI CCNNGG 2 cut(s) 8, 99
BseGI GGATG 2 cut(s) 260, 295
BseLI CCNNNNNNNGG 1 cut(s) 34
BseMII CTCAG 1 cut(s) 91
BseNI ACTGG 1 cut(s) 207
BseRI GAGGAG 2 cut(s) 130, 413
BseXI GCAGC 1 cut(s) 213
BsgI GTGCAG 2 cut(s) 462, 467
Bsh1285I CGRYCG 1 cut(s) 52
BshTI ACCGGT 1 cut(s) 27
BsiEI CGRYCG 1 cut(s) 52
BsiSI CCGG 2 cut(s) 28, 48
BslI CCNNNNNNNGG 1 cut(s) 34
Bsp143I GATC 2 cut(s) 280, 405
BspACI CCGC 1 cut(s) 232
BspCNI CTCAG 1 cut(s) 90
BspLI GGNNCC 2 cut(s) 6, 266
BspPI GGATC 1 cut(s) 400
BsrFI RCCGGY 2 cut(s) 27, 47
BsrI ACTGG 1 cut(s) 207
BssAI RCCGGY 2 cut(s) 27, 47
BssECI CCNNGG 2 cut(s) 8, 99
BssMI GATC 2 cut(s) 280, 405
Bst4CI ACNGT 1 cut(s) 385
Bst6I CTCTTC 1 cut(s) 130
BstC8I GCNNGC 1 cut(s) 204
BstDEI CTNAG 1 cut(s) 77
BstDSI CCRYGG 1 cut(s) 99
BstF5I GGATG 2 cut(s) 260, 295
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 2 cut(s) 283, 408
BstMBI GATC 2 cut(s) 280, 405
BstMCI CGRYCG 1 cut(s) 52
BstMWI GCNNNNNNNGC 2 cut(s) 44, 212
BstV1I GCAGC 1 cut(s) 213
BstXI CCANNNNNNTGG 1 cut(s) 106
BtgI CCRYGG 1 cut(s) 99
BtsCI GGATG 2 cut(s) 260, 295
BtsI GCAGTG 1 cut(s) 450
BtsIMutI CAGTG 2 cut(s) 214, 450
Cac8I GCNNGC 1 cut(s) 204
CfoI GCGC 1 cut(s) 40
Cfr10I RCCGGY 2 cut(s) 27, 47
CspAI ACCGGT 1 cut(s) 27
CviAII CATG 2 cut(s) 166, 475
CviJI RGCY 7 cut(s) 5, 73, 81, 116, 206, 226, 403
CviKI_1 RGCY 7 cut(s) 5, 73, 81, 116, 206, 226, 403
DdeI CTNAG 1 cut(s) 77
DpnI GATC 2 cut(s) 282, 407
DpnII GATC 2 cut(s) 280, 405
Eam1104I CTCTTC 1 cut(s) 130
EarI CTCTTC 1 cut(s) 130
FaeI CATG 2 cut(s) 169, 478
FaiI YATR 7 cut(s) 167, 273, 361, 363, 393, 476, 487
FatI CATG 2 cut(s) 165, 474
FauNDI CATATG 1 cut(s) 361
FblI GTMKAC 1 cut(s) 348
Fnu4HI GCNGC 1 cut(s) 227
FokI GGATG 2 cut(s) 267, 302
Fsp4HI GCNGC 1 cut(s) 227
GlaI GCGC 1 cut(s) 39
GluI GCNGC 1 cut(s) 227
HapII CCGG 2 cut(s) 28, 48
HhaI GCGC 1 cut(s) 40
Hin1II CATG 2 cut(s) 169, 478
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HincII GTYRAC 2 cut(s) 33, 349
HindII GTYRAC 2 cut(s) 33, 349
HinfI GANTC 2 cut(s) 19, 54
HpaII CCGG 2 cut(s) 28, 48
HphI GGTGA 1 cut(s) 17
Hpy166II GTNNAC 3 cut(s) 33, 163, 349
Hpy188I TCNGA 2 cut(s) 9, 186
Hpy188III TCNNGA 2 cut(s) 305, 322
Hpy8I GTNNAC 3 cut(s) 33, 163, 349
HpyAV CCTTC 2 cut(s) 129, 323
HpyCH4III ACNGT 1 cut(s) 385
HpyCH4IV ACGT 1 cut(s) 14
HpyCH4V TGCA 4 cut(s) 202, 443, 448, 456
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 212
HpyF3I CTNAG 1 cut(s) 77
HpySE526I ACGT 1 cut(s) 14
Hsp92II CATG 2 cut(s) 169, 478
HspAI GCGC 1 cut(s) 38
Kzo9I GATC 2 cut(s) 280, 405
LmnI GCTCC 3 cut(s) 10, 234, 400
Lsp1109I GCAGC 1 cut(s) 213
MaeII ACGT 1 cut(s) 14
MaeIII GTNAC 1 cut(s) 424
MalI GATC 2 cut(s) 282, 407
MboI GATC 2 cut(s) 280, 405
MboII GAAGA 6 cut(s) 14, 117, 120, 122, 123, 126
MluCI AATT 2 cut(s) 338, 482
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 7 cut(s) 3, 63, 106, 133, 148, 151, 391
MspI CCGG 2 cut(s) 28, 48
MwoI GCNNNNNNNGC 2 cut(s) 44, 212
NdeI CATATG 1 cut(s) 361
NdeII GATC 2 cut(s) 280, 405
NlaIII CATG 2 cut(s) 169, 478
NlaIV GGNNCC 2 cut(s) 6, 266
NmuCI GTSAC 1 cut(s) 424
PfeI GAWTC 2 cut(s) 19, 54
PinAI ACCGGT 1 cut(s) 27
PkrI GCNGC 1 cut(s) 228
PspN4I GGNNCC 2 cut(s) 6, 266
SalI GTCGAC 1 cut(s) 347
SatI GCNGC 1 cut(s) 227
Sau3AI GATC 2 cut(s) 280, 405
SetI ASST 5 cut(s) 17, 83, 228, 253, 405
SmlI CTYRAG 1 cut(s) 235
SmoI CTYRAG 1 cut(s) 235
Sse9I AATT 2 cut(s) 338, 482
SsiI CCGC 1 cut(s) 232
SspI AATATT 1 cut(s) 355
TaaI ACNGT 1 cut(s) 385
TaiI ACGT 1 cut(s) 17
TaqI TCGA 3 cut(s) 52, 321, 348
TasI AATT 2 cut(s) 338, 482
TfiI GAWTC 2 cut(s) 19, 54
TscAI CASTG 2 cut(s) 214, 450
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 1 cut(s) 226
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 3 cut(s) 206, 348, 463
TspGWI ACGGA 2 cut(s) 185, 398
TspRI CASTG 2 cut(s) 214, 450
XmiI GTMKAC 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.