Rh3DG209200

Iron-sulfur assembly protein IscA-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0006268
Physical Location & Seq
Reverse (-)
19362313 .. 19364725
2413 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG209200.1

Sequence Viewer

Length: 492 bp
ATGGCTCCGAGGACGTTGATTCTTCACCGGTTGACGGCGCACTTTGCCGGTCGAATCAGGCAGAACCGGAGGCTTCTCAGCTCTCATTTTTATTCCTCTACGGCGTTGGAAGAAGCCCCTTCCTCTTCCTCTTCTTCTTCCTCCTCGCCGAACTTGGACGGCGTTTACATGACGGACACTTGTGTTCAGAAAATGAAACAACTGCAAGCCAGTGAAGCACAGGAAAAGCTGCTCCGCTTGAGTGTAGAAACAGGTGGATGTTCTGGGTTCCAATATGTTTTTGATCTGGTGGATGACAAAACCAATCCAGATGACAGGGTGTTCGAGAAAGAAGGAGTAAAATTGGTAGTCGACAATATTTCATATGATTTTTTGAAAGGGGCAACCGTTGATTATGTTGAGGAGCTGATCCGTTCTGCTTTCCAAGTGACAGAAAATCCAAGTGCAGTGCAGGGTTGCAGTTGTAAAAGTTCTTTCATGGTGAAATTATAG

Protein Analysis

163

Amino Acids

18.09

Weight (kDa)

5.92

Isoelectric Point (pI)

50.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fe-S_biosyn PF01521 56 - 156 3.4e-16 Iron-sulphur cluster biosynthesis
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 351
AciI CCGC 1 cut(s) 235
AclWI GGATC 1 cut(s) 403
AfiI CCNNNNNNNGG 1 cut(s) 34
AgeI ACCGGT 1 cut(s) 27
AgsI TTSAA 1 cut(s) 376
AluBI AGCT 3 cut(s) 81, 229, 406
AluI AGCT 3 cut(s) 81, 229, 406
AlwI GGATC 1 cut(s) 403
ApeKI GCWGC 1 cut(s) 229
ArsI GACNNNNNNTTYG 2 cut(s) 305, 337
AsiGI ACCGGT 1 cut(s) 27
AspLEI GCGC 1 cut(s) 40
AsuHPI GGTGA 1 cut(s) 17
BbvI GCAGC 1 cut(s) 216
BceAI ACGGC 3 cut(s) 51, 117, 175
BisI GCNGC 1 cut(s) 230
BlsI GCNGC 1 cut(s) 231
BmiI GGNNCC 2 cut(s) 6, 269
BpuEI CTTGAG 1 cut(s) 259
BsaJI CCNNGG 1 cut(s) 8
BsaWI WCCGGW 2 cut(s) 27, 66
Bsc4I CCNNNNNNNGG 1 cut(s) 34
Bse118I RCCGGY 2 cut(s) 27, 47
Bse1I ACTGG 1 cut(s) 210
BseDI CCNNGG 1 cut(s) 8
BseGI GGATG 2 cut(s) 263, 298
BseLI CCNNNNNNNGG 1 cut(s) 34
BseMII CTCAG 1 cut(s) 91
BseNI ACTGG 1 cut(s) 210
BseRI GAGGAG 2 cut(s) 133, 416
BseXI GCAGC 1 cut(s) 216
BsgI GTGCAG 2 cut(s) 465, 470
Bsh1285I CGRYCG 1 cut(s) 52
BshTI ACCGGT 1 cut(s) 27
BsiEI CGRYCG 1 cut(s) 52
BsiSI CCGG 3 cut(s) 28, 48, 67
BslI CCNNNNNNNGG 1 cut(s) 34
Bsp143I GATC 2 cut(s) 283, 408
BspACI CCGC 1 cut(s) 235
BspCNI CTCAG 1 cut(s) 90
BspLI GGNNCC 2 cut(s) 6, 269
BspPI GGATC 1 cut(s) 403
BsrFI RCCGGY 2 cut(s) 27, 47
BsrI ACTGG 1 cut(s) 210
BssAI RCCGGY 2 cut(s) 27, 47
BssECI CCNNGG 1 cut(s) 8
BssMI GATC 2 cut(s) 283, 408
Bst4CI ACNGT 1 cut(s) 388
Bst6I CTCTTC 2 cut(s) 130, 136
BstC8I GCNNGC 1 cut(s) 207
BstDEI CTNAG 1 cut(s) 77
BstF5I GGATG 2 cut(s) 263, 298
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 2 cut(s) 286, 411
BstMBI GATC 2 cut(s) 283, 408
BstMCI CGRYCG 1 cut(s) 52
BstMWI GCNNNNNNNGC 2 cut(s) 44, 215
BstV1I GCAGC 1 cut(s) 216
BtsCI GGATG 2 cut(s) 263, 298
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 2 cut(s) 217, 453
Cac8I GCNNGC 1 cut(s) 207
CfoI GCGC 1 cut(s) 40
Cfr10I RCCGGY 2 cut(s) 27, 47
CspAI ACCGGT 1 cut(s) 27
CviAII CATG 2 cut(s) 169, 478
CviJI RGCY 7 cut(s) 5, 73, 81, 116, 209, 229, 406
CviKI_1 RGCY 7 cut(s) 5, 73, 81, 116, 209, 229, 406
DdeI CTNAG 1 cut(s) 77
DpnI GATC 2 cut(s) 285, 410
DpnII GATC 2 cut(s) 283, 408
Eam1104I CTCTTC 2 cut(s) 130, 136
EarI CTCTTC 2 cut(s) 130, 136
FaeI CATG 2 cut(s) 172, 481
FaiI YATR 7 cut(s) 170, 276, 364, 366, 396, 479, 490
FatI CATG 2 cut(s) 168, 477
FauNDI CATATG 1 cut(s) 364
FblI GTMKAC 1 cut(s) 351
Fnu4HI GCNGC 1 cut(s) 230
FokI GGATG 2 cut(s) 270, 305
Fsp4HI GCNGC 1 cut(s) 230
GlaI GCGC 1 cut(s) 39
GluI GCNGC 1 cut(s) 230
HapII CCGG 3 cut(s) 28, 48, 67
HhaI GCGC 1 cut(s) 40
Hin1II CATG 2 cut(s) 172, 481
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HincII GTYRAC 2 cut(s) 33, 352
HindII GTYRAC 2 cut(s) 33, 352
HinfI GANTC 2 cut(s) 19, 54
HpaII CCGG 3 cut(s) 28, 48, 67
HphI GGTGA 1 cut(s) 17
Hpy166II GTNNAC 3 cut(s) 33, 166, 352
Hpy188I TCNGA 2 cut(s) 9, 189
Hpy188III TCNNGA 2 cut(s) 308, 325
Hpy8I GTNNAC 3 cut(s) 33, 166, 352
HpyAV CCTTC 2 cut(s) 129, 326
HpyCH4III ACNGT 1 cut(s) 388
HpyCH4IV ACGT 1 cut(s) 14
HpyCH4V TGCA 4 cut(s) 205, 446, 451, 459
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 215
HpyF3I CTNAG 1 cut(s) 77
HpySE526I ACGT 1 cut(s) 14
Hsp92II CATG 2 cut(s) 172, 481
HspAI GCGC 1 cut(s) 38
Kzo9I GATC 2 cut(s) 283, 408
LmnI GCTCC 3 cut(s) 10, 237, 403
Lsp1109I GCAGC 1 cut(s) 216
MaeII ACGT 1 cut(s) 14
MaeIII GTNAC 1 cut(s) 427
MalI GATC 2 cut(s) 285, 410
MboI GATC 2 cut(s) 283, 408
MboII GAAGA 6 cut(s) 14, 117, 122, 123, 126, 129
MluCI AATT 2 cut(s) 341, 485
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 8 cut(s) 3, 63, 106, 133, 139, 151, 154, 394
MspI CCGG 3 cut(s) 28, 48, 67
MwoI GCNNNNNNNGC 2 cut(s) 44, 215
NdeI CATATG 1 cut(s) 364
NdeII GATC 2 cut(s) 283, 408
NlaIII CATG 2 cut(s) 172, 481
NlaIV GGNNCC 2 cut(s) 6, 269
NmuCI GTSAC 1 cut(s) 427
PfeI GAWTC 2 cut(s) 19, 54
PinAI ACCGGT 1 cut(s) 27
PkrI GCNGC 1 cut(s) 231
PspN4I GGNNCC 2 cut(s) 6, 269
SalI GTCGAC 1 cut(s) 350
SatI GCNGC 1 cut(s) 230
Sau3AI GATC 2 cut(s) 283, 408
SetI ASST 5 cut(s) 17, 83, 231, 256, 408
SmlI CTYRAG 1 cut(s) 238
SmoI CTYRAG 1 cut(s) 238
Sse9I AATT 2 cut(s) 341, 485
SsiI CCGC 1 cut(s) 235
SspI AATATT 1 cut(s) 358
TaaI ACNGT 1 cut(s) 388
TaiI ACGT 1 cut(s) 17
TaqI TCGA 3 cut(s) 52, 324, 351
TasI AATT 2 cut(s) 341, 485
TfiI GAWTC 2 cut(s) 19, 54
TscAI CASTG 2 cut(s) 217, 453
TseFI GTSAC 1 cut(s) 427
TseI GCWGC 1 cut(s) 229
Tsp45I GTSAC 1 cut(s) 427
TspDTI ATGAA 3 cut(s) 209, 351, 466
TspGWI ACGGA 2 cut(s) 188, 401
TspRI CASTG 2 cut(s) 217, 453
XmiI GTMKAC 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.