Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0006690

Physical Location & Seq

Genomic Coordinates Forward (+)

5233402 .. 5237650

Gene Structure 4249 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1041200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 750 bp

ATGACAGCTTCTTCCGGTGAAAACTCTCTGGACAGCAAAAGTTCACTCTCTGGAGTTGCTCCACTTGAAGCAGTATTATTTGATATTGATGGAACGCTGTGTGATTCGGATCCAATTCATTACTATGCCTTCCGTGAAATGCTACCAGAGATAGGTTTCAACGGAGGTGTTCCGATTACCGAGGAGTACTATATTGAGAATTTCGCTGGGAAGCATAATGATGATGTTGCCAGGATACTCTTTCCTGATGACTTCGAAAGAGGCTTAAAGTTCACAGAAGACAAGGAAGCCATGTTTCGCAGATTGGCCGTAGATAAATTGAAGCCTGTGAATGGTCTCTATAAAGTGAAGAAATGGATTGAAGATCGTGGGTTGAAACGGGCTGCTGTTACTAATGCTCCAAGAGTGAATGCAGAGCTGATGATTTCAATGCTTGGCCTCTCAGATTTTTTTCAAGGTCTTGTCATTGGGAATGAGTGTGAGCATGCTAAGCCACACCCCGAGCCCTACTTGAAGGGTCTTGAAATACTCAAAGCATCAAAAGAACACACTTTCGTGTTCGAGGATTCTGTCTCGGGGATTAAAGCAGCAGTTGCAGCCGGTATGCCAGTCGTTGGATTAACCACTGGGAATCCAGAACACATATTAGTGGAAGCAAAACCCACCTTTCTCATAAAGGACTATGAGGACCCAAAGTTGTGGGCGGCATTGGAAGAACTTGATAAGAAGAGCGGTACAAGTAGAGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003850 GO:0004346 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019203 GO:0042578 GO:0044237 GO:0050308 GO:0050309

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HAD_2

Protein Analysis

250

Amino Acids

27.52

Weight (kDa)

5.09

Isoelectric Point (pI)

38.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydrolase	PF00702	23 - 201	6.9e-26	haloacid dehalogenase-like hydrolase
HAD_2	PF13419	25 - 206	6.6e-29	Haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006690)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38740
fragaria_vesca	FvH4_5g21050 FvH4_5g21050 FvH4_5g21050 FvH4_5g21050 FvH4_5g21050
malus_domestica	MD06G1041200.v1.1 MD12G1227700.v1.1 MD15G1033000.v1.1
prunus_persica	Prupe.5G047500_v2.0.a1 Prupe.5G047600_v2.0.a1 Prupe.5G047600_v2.0.a1
pyrus_communis	pycom06g03420
rosa_chinensis	RchiOBHm_Chr7g0208111
rosa_laevigata	RLG00000003254
rosa_multiflora	Rmu_sc0000706.1_g000042
rosa_roxburghii	Rroxscaffold_3G00250810
rosa_rugosa	Rorug07G0101400 Rorug07G0101500 Rorug07G0101600 Rorug07G0101700
rosa_samantha	Rh7AG234500 Rh7BG231100 Rh7CG250700 Rh7DG241500
rosa_wichuraiana	Rw7G020080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	614
AccBSI	CCGCTC	1 cut(s)	732
AciI	CCGC	2 cut(s)	704, 732
AclWI	GGATC	2 cut(s)	104, 117
AcoI	YGGCCR	1 cut(s)	306
AcsI	RAATTY	1 cut(s)	199
AfaI	GTAC	2 cut(s)	188, 736
AfiI	CCNNNNNNNGG	3 cut(s)	152, 332, 614
AgsI	TTSAA	9 cut(s)	68, 160, 322, 362, 376, 429, 455, 514, 524
AjnI	CCWGG	1 cut(s)	230
AleI	CACNNNNGTG	1 cut(s)	554
AluBI	AGCT	2 cut(s)	8, 418
AluI	AGCT	2 cut(s)	8, 418
Alw26I	GTCTC	2 cut(s)	341, 577
AlwI	GGATC	2 cut(s)	104, 117
Ama87I	CYCGRG	2 cut(s)	500, 574
AoxI	GGCC	2 cut(s)	306, 436
ApeKI	GCWGC	3 cut(s)	383, 587, 596
ApoI	RAATTY	1 cut(s)	199
AspS9I	GGNCC	1 cut(s)	688
AsuHPI	GGTGA	1 cut(s)	29
AsuII	TTCGAA	1 cut(s)	255
AvaI	CYCGRG	2 cut(s)	500, 574
AvaII	GGWCC	1 cut(s)	688
BamHI	GGATCC	1 cut(s)	109
BanII	GRGCYC	1 cut(s)	507
BbsI	GAAGAC	1 cut(s)	285
BbvI	GCAGC	3 cut(s)	370, 599, 608
BccI	CCATC	1 cut(s)	83
BceAI	ACGGC	1 cut(s)	293
BciT130I	CCWGG	1 cut(s)	232
BciVI	GTATCC	1 cut(s)	228
BcoDI	GTCTC	2 cut(s)	341, 577
BfuI	GTATCC	1 cut(s)	228
BisI	GCNGC	4 cut(s)	384, 588, 597, 705
BlpI	GCTNAGC	1 cut(s)	489
BlsI	GCNGC	4 cut(s)	385, 589, 598, 706
BmcAI	AGTACT	1 cut(s)	188
Bme1390I	CCNGG	1 cut(s)	232
Bme18I	GGWCC	1 cut(s)	688
BmeT110I	CYCGRG	2 cut(s)	500, 574
BmgT120I	GGNCC	1 cut(s)	688
BmiI	GGNNCC	2 cut(s)	111, 690
BmrFI	CCNGG	1 cut(s)	232
BmrI	ACTGGG	1 cut(s)	636
BmsI	GCATC	1 cut(s)	545
BmuI	ACTGGG	1 cut(s)	636
BpiI	GAAGAC	1 cut(s)	285
BpmI	CTGGAG	1 cut(s)	72
Bpu1102I	GCTNAGC	1 cut(s)	489
Bpu14I	TTCGAA	1 cut(s)	255
BsaBI	GATNNNNATC	1 cut(s)	108
BsaI	GGTCTC	1 cut(s)	341
BsaJI	CCNNGG	1 cut(s)	180
BsaWI	WCCGGW	1 cut(s)	14
BsaXI	ACNNNNNCTCC	2 cut(s)	382, 412
Bsc4I	CCNNNNNNNGG	3 cut(s)	152, 332, 614
Bse118I	RCCGGY	1 cut(s)	599
Bse1I	ACTGG	2 cut(s)	608, 631
Bse8I	GATNNNNATC	1 cut(s)	108
BseBI	CCWGG	1 cut(s)	232
BseDI	CCNNGG	1 cut(s)	180
BseJI	GATNNNNATC	1 cut(s)	108
BseLI	CCNNNNNNNGG	3 cut(s)	152, 332, 614
BseMII	CTCAG	1 cut(s)	456
BseNI	ACTGG	2 cut(s)	608, 631
BseRI	GAGGAG	1 cut(s)	197
BseXI	GCAGC	3 cut(s)	370, 599, 608
BseYI	CCCAGC	1 cut(s)	206
BshFI	GGCC	2 cut(s)	308, 438
BsiHKCI	CYCGRG	2 cut(s)	500, 574
BsiSI	CCGG	2 cut(s)	15, 600
BslI	CCNNNNNNNGG	3 cut(s)	152, 332, 614
BsmAI	GTCTC	2 cut(s)	341, 577
BsmI	GAATGC	1 cut(s)	415
BsnI	GGCC	2 cut(s)	308, 438
Bso31I	GGTCTC	1 cut(s)	341
BsoBI	CYCGRG	2 cut(s)	500, 574
Bsp119I	TTCGAA	1 cut(s)	255
Bsp1286I	GDGCHC	1 cut(s)	507
Bsp143I	GATC	2 cut(s)	109, 364
Bsp1720I	GCTNAGC	1 cut(s)	489
BspACI	CCGC	2 cut(s)	704, 732
BspANI	GGCC	2 cut(s)	308, 438
BspCNI	CTCAG	1 cut(s)	455
BspLI	GGNNCC	2 cut(s)	111, 690
BspPI	GGATC	2 cut(s)	104, 117
BspQI	GCTCTTC	1 cut(s)	722
BspT104I	TTCGAA	1 cut(s)	255
BspTNI	GGTCTC	1 cut(s)	341
BsrBI	CCGCTC	1 cut(s)	732
BsrFI	RCCGGY	1 cut(s)	599
BsrI	ACTGG	2 cut(s)	608, 631
BssAI	RCCGGY	1 cut(s)	599
BssECI	CCNNGG	1 cut(s)	180
BssMI	GATC	2 cut(s)	109, 364
Bst2UI	CCWGG	1 cut(s)	232
Bst6I	CTCTTC	1 cut(s)	722
BstAPI	GCANNNNNTGC	1 cut(s)	593
BstBI	TTCGAA	1 cut(s)	255
BstC8I	GCNNGC	1 cut(s)	486
BstDEI	CTNAG	2 cut(s)	442, 489
BstKTI	GATC	2 cut(s)	112, 367
BstMAI	GTCTC	2 cut(s)	341, 577
BstMBI	GATC	2 cut(s)	109, 364
BstMWI	GCNNNNNNNGC	3 cut(s)	490, 593, 596
BstNI	CCWGG	1 cut(s)	232
BstNSI	RCATGY	1 cut(s)	488
BstSCI	CCNGG	1 cut(s)	230
BstV1I	GCAGC	3 cut(s)	370, 599, 608
BstV2I	GAAGAC	1 cut(s)	285
BstX2I	RGATCY	1 cut(s)	109
BstXI	CCANNNNNNTGG	1 cut(s)	699
BstYI	RGATCY	1 cut(s)	109
BsuI	GTATCC	1 cut(s)	228
BsuRI	GGCC	2 cut(s)	308, 438
BtsIMutI	CAGTG	1 cut(s)	624
Cac8I	GCNNGC	1 cut(s)	486
Cfr10I	RCCGGY	1 cut(s)	599
Cfr13I	GGNCC	1 cut(s)	688
Csp6I	GTAC	2 cut(s)	187, 735
CviAII	CATG	3 cut(s)	292, 485, 747
CviQI	GTAC	2 cut(s)	187, 735
DdeI	CTNAG	2 cut(s)	442, 489
DpnI	GATC	2 cut(s)	111, 366
DpnII	GATC	2 cut(s)	109, 364
EaeI	YGGCCR	1 cut(s)	306
Eam1104I	CTCTTC	1 cut(s)	722
EarI	CTCTTC	1 cut(s)	722
Eco24I	GRGCYC	1 cut(s)	507
Eco31I	GGTCTC	1 cut(s)	341
Eco47I	GGWCC	1 cut(s)	688
Eco88I	CYCGRG	2 cut(s)	500, 574
EcoO109I	RGGNCCY	1 cut(s)	688
EcoRII	CCWGG	1 cut(s)	230
EcoT38I	GRGCYC	1 cut(s)	507
FaeI	CATG	3 cut(s)	295, 488, 750
FatI	CATG	3 cut(s)	291, 484, 746
Fnu4HI	GCNGC	4 cut(s)	384, 588, 597, 705
FriOI	GRGCYC	1 cut(s)	507
Fsp4HI	GCNGC	4 cut(s)	384, 588, 597, 705
GluI	GCNGC	4 cut(s)	384, 588, 597, 705
GsaI	CCCAGC	1 cut(s)	210
GsuI	CTGGAG	1 cut(s)	72
HaeIII	GGCC	2 cut(s)	308, 438
HapII	CCGG	2 cut(s)	15, 600
Hin1II	CATG	3 cut(s)	295, 488, 750
HinfI	GANTC	3 cut(s)	104, 566, 631
HpaII	CCGG	2 cut(s)	15, 600
HphI	GGTGA	1 cut(s)	29
Hpy166II	GTNNAC	2 cut(s)	44, 273
Hpy188I	TCNGA	3 cut(s)	109, 174, 445
Hpy188III	TCNNGA	5 cut(s)	29, 51, 245, 521, 635
Hpy8I	GTNNAC	2 cut(s)	44, 273
HpyAV	CCTTC	2 cut(s)	139, 508
HpyCH4V	TGCA	2 cut(s)	413, 596
HpyF10VI	GCNNNNNNNGC	3 cut(s)	490, 593, 596
HpyF3I	CTNAG	2 cut(s)	442, 489
Hsp92II	CATG	3 cut(s)	295, 488, 750
Kzo9I	GATC	2 cut(s)	109, 364
LguI	GCTCTTC	1 cut(s)	722
LmnI	GCTCC	2 cut(s)	64, 403
Lsp1109I	GCAGC	3 cut(s)	370, 599, 608
LweI	GCATC	1 cut(s)	545
MaeIII	GTNAC	1 cut(s)	388
MalI	GATC	2 cut(s)	111, 366
MbiI	CCGCTC	1 cut(s)	732
MboI	GATC	2 cut(s)	109, 364
MboII	GAAGA	6 cut(s)	3, 290, 361, 374, 725, 739
MflI	RGATCY	1 cut(s)	109
MhlI	GDGCHC	1 cut(s)	507
MluCI	AATT	3 cut(s)	114, 199, 317
MmeI	TCCRAC	1 cut(s)	595
MnlI	CCTC	6 cut(s)	158, 175, 254, 449, 556, 679
MseI	TTAA	3 cut(s)	266, 582, 620
MslI	CAYNNNNRTG	4 cut(s)	123, 219, 554, 647
MspI	CCGG	2 cut(s)	15, 600
MspR9I	CCNGG	1 cut(s)	232
Mva1269I	GAATGC	1 cut(s)	415
MvaI	CCWGG	1 cut(s)	232
MwoI	GCNNNNNNNGC	3 cut(s)	490, 593, 596
NdeII	GATC	2 cut(s)	109, 364
NlaIII	CATG	3 cut(s)	295, 488, 750
NlaIV	GGNNCC	2 cut(s)	111, 690
NspI	RCATGY	1 cut(s)	488
NspV	TTCGAA	1 cut(s)	255
OliI	CACNNNNGTG	1 cut(s)	554
PaeI	GCATGC	1 cut(s)	488
PciSI	GCTCTTC	1 cut(s)	722
PctI	GAATGC	1 cut(s)	415
PfeI	GAWTC	3 cut(s)	104, 566, 631
PflMI	CCANNNNNTGG	1 cut(s)	614
PkrI	GCNGC	4 cut(s)	385, 589, 598, 706
PpuMI	RGGWCCY	1 cut(s)	688
Psp5II	RGGWCCY	1 cut(s)	688
Psp6I	CCWGG	1 cut(s)	230
PspFI	CCCAGC	1 cut(s)	206
PspGI	CCWGG	1 cut(s)	230
PspN4I	GGNNCC	2 cut(s)	111, 690
PspPI	GGNCC	1 cut(s)	688
PspPPI	RGGWCCY	1 cut(s)	688
PsuI	RGATCY	1 cut(s)	109
RsaI	GTAC	2 cut(s)	188, 736
RsaNI	GTAC	2 cut(s)	187, 735
RseI	CAYNNNNRTG	4 cut(s)	123, 219, 554, 647
SapI	GCTCTTC	1 cut(s)	722
SaqAI	TTAA	3 cut(s)	266, 582, 620
SatI	GCNGC	4 cut(s)	384, 588, 597, 705
Sau3AI	GATC	2 cut(s)	109, 364
Sau96I	GGNCC	1 cut(s)	688
ScaI	AGTACT	1 cut(s)	188
ScrFI	CCNGG	1 cut(s)	232
SduI	GDGCHC	1 cut(s)	507
SetI	ASST	6 cut(s)	10, 157, 169, 420, 460, 668
SfaNI	GCATC	1 cut(s)	545
SfuI	TTCGAA	1 cut(s)	255
SinI	GGWCC	1 cut(s)	688
SmiMI	CAYNNNNRTG	4 cut(s)	123, 219, 554, 647
SphI	GCATGC	1 cut(s)	488
Sse9I	AATT	3 cut(s)	114, 199, 317
SsiI	CCGC	2 cut(s)	704, 732
StyD4I	CCNGG	1 cut(s)	230
TaqI	TCGA	2 cut(s)	255, 561
TasI	AATT	3 cut(s)	114, 199, 317
TatI	WGTACW	1 cut(s)	186
TauI	GCSGC	1 cut(s)	707
TfiI	GAWTC	3 cut(s)	104, 566, 631
Tru1I	TTAA	3 cut(s)	266, 582, 620
Tru9I	TTAA	3 cut(s)	266, 582, 620
TscAI	CASTG	1 cut(s)	631
TseI	GCWGC	3 cut(s)	383, 587, 596
TspDTI	ATGAA	1 cut(s)	107
TspGWI	ACGGA	2 cut(s)	122, 177
TspRI	CASTG	1 cut(s)	631
Van91I	CCANNNNNTGG	1 cut(s)	614
VpaK11BI	GGWCC	1 cut(s)	688
XapI	RAATTY	1 cut(s)	199
XceI	RCATGY	1 cut(s)	488
ZrmI	AGTACT	1 cut(s)	188

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.