MD15G1033000.v1.1

Haloacid dehalogenase-like hydrolase domain-containing protein Sgpp

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006690
Physical Location & Seq
Forward (+)
2311496 .. 2315834
4339 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1033000.v1.1.491

Sequence Viewer

Length: 495 bp
ATGGAAGCCTGGTGTTGTACGTTGGATTCAGAGTGCAGCATGACAATTGGAACCAGCAAAAGTTCACTCTCTGGAGTTGCTCCACTTGAAGTTGTATTATTTGATATTGATGGAACGCTGTGTGATTCGGATCAAATTCATTACTATGCCTTTCGTGAAATGCTACCAGAGATAGGTTTCAACGGAGGTGTTCCGATTACTGAGGAGTACTATATTGAGAATTTTGCTGGGAAGCATAACGATGATGTTGCCAGGATACTGTTTCCTGATGACTTAGAAAGAGGCTTAAAGTTCACAGAAGACAAGGAAGCCATGTTTCGCAGATTGGCCGTAGATAAATTGAAGCCTGTGAATGGTCTCTATAAAGTGAAGAAATGGATTGAAGATCGTGGGTTGAAACGGACAAAATCCATGACCTCATTGGAACTCATACTGATGTTCCAGCATCCGATATGGGCACCAATGCTCAGACCATCCTTAAATATCTATTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003850 GO:0004346 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019203 GO:0042578 GO:0044237 GO:0050308 GO:0050309
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2

Protein Analysis

165

Amino Acids

18.84

Weight (kDa)

5.1

Isoelectric Point (pI)

51.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAD_2 PF13419 32 - 133 6.2e-07 Haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 457
AclWI GGATC 1 cut(s) 138
AcoI YGGCCR 1 cut(s) 327
AcsI RAATTY 2 cut(s) 135, 220
AfaI GTAC 2 cut(s) 19, 209
AfiI CCNNNNNNNGG 2 cut(s) 173, 353
AgsI TTSAA 5 cut(s) 89, 181, 343, 383, 397
AjnI CCWGG 2 cut(s) 8, 251
Alw26I GTCTC 1 cut(s) 362
AlwI GGATC 1 cut(s) 138
AoxI GGCC 1 cut(s) 327
ApeKI GCWGC 1 cut(s) 36
ApoI RAATTY 2 cut(s) 135, 220
BaeGI GKGCMC 1 cut(s) 460
BanI GGYRCC 1 cut(s) 457
BbsI GAAGAC 1 cut(s) 306
BbvI GCAGC 1 cut(s) 48
BccI CCATC 2 cut(s) 104, 481
BceAI ACGGC 1 cut(s) 314
BcgI CGANNNNNNTGC 2 cut(s) 230, 264
BciT130I CCWGG 2 cut(s) 10, 253
BciVI GTATCC 1 cut(s) 249
BcoDI GTCTC 1 cut(s) 362
BfuI GTATCC 1 cut(s) 249
BisI GCNGC 1 cut(s) 37
BlsI GCNGC 1 cut(s) 38
BmcAI AGTACT 1 cut(s) 209
Bme1390I CCNGG 2 cut(s) 10, 253
BmiI GGNNCC 2 cut(s) 52, 459
BmrFI CCNGG 2 cut(s) 10, 253
BmsI GCATC 1 cut(s) 454
BpiI GAAGAC 1 cut(s) 306
BpmI CTGGAG 1 cut(s) 93
BsaBI GATNNNNATC 1 cut(s) 129
BsaI GGTCTC 1 cut(s) 362
Bsc4I CCNNNNNNNGG 2 cut(s) 173, 353
Bse8I GATNNNNATC 1 cut(s) 129
BseBI CCWGG 2 cut(s) 10, 253
BseGI GGATG 2 cut(s) 445, 473
BseJI GATNNNNATC 1 cut(s) 129
BseLI CCNNNNNNNGG 2 cut(s) 173, 353
BseMII CTCAG 2 cut(s) 192, 481
BseRI GAGGAG 1 cut(s) 218
BseSI GKGCMC 1 cut(s) 460
BseXI GCAGC 1 cut(s) 48
BseYI CCCAGC 1 cut(s) 227
BsgI GTGCAG 1 cut(s) 55
BshFI GGCC 1 cut(s) 329
BshNI GGYRCC 1 cut(s) 457
BslI CCNNNNNNNGG 2 cut(s) 173, 353
BsmAI GTCTC 1 cut(s) 362
BsnI GGCC 1 cut(s) 329
Bso31I GGTCTC 1 cut(s) 362
Bsp1286I GDGCHC 1 cut(s) 460
Bsp143I GATC 2 cut(s) 130, 385
BspANI GGCC 1 cut(s) 329
BspCNI CTCAG 2 cut(s) 193, 480
BspLI GGNNCC 2 cut(s) 52, 459
BspPI GGATC 1 cut(s) 138
BspT107I GGYRCC 1 cut(s) 457
BspTNI GGTCTC 1 cut(s) 362
BssMI GATC 2 cut(s) 130, 385
Bst2UI CCWGG 2 cut(s) 10, 253
Bst4CI ACNGT 1 cut(s) 261
BstDEI CTNAG 3 cut(s) 201, 274, 467
BstF5I GGATG 2 cut(s) 445, 473
BstKTI GATC 2 cut(s) 133, 388
BstMAI GTCTC 1 cut(s) 362
BstMBI GATC 2 cut(s) 130, 385
BstNI CCWGG 2 cut(s) 10, 253
BstSCI CCNGG 2 cut(s) 8, 251
BstSLI GKGCMC 1 cut(s) 460
BstV1I GCAGC 1 cut(s) 48
BstV2I GAAGAC 1 cut(s) 306
BsuI GTATCC 1 cut(s) 249
BsuRI GGCC 1 cut(s) 329
BtsCI GGATG 2 cut(s) 445, 473
Csp6I GTAC 2 cut(s) 18, 208
CviAII CATG 3 cut(s) 40, 313, 412
CviJI RGCY 5 cut(s) 8, 285, 311, 329, 346
CviKI_1 RGCY 5 cut(s) 8, 285, 311, 329, 346
CviQI GTAC 2 cut(s) 18, 208
DdeI CTNAG 3 cut(s) 201, 274, 467
DpnI GATC 2 cut(s) 132, 387
DpnII GATC 2 cut(s) 130, 385
EaeI YGGCCR 1 cut(s) 327
Eco31I GGTCTC 1 cut(s) 362
EcoRII CCWGG 2 cut(s) 8, 251
FaeI CATG 3 cut(s) 43, 316, 415
FaiI YATR 9 cut(s) 41, 147, 213, 237, 314, 363, 413, 431, 454
FatI CATG 3 cut(s) 39, 312, 411
Fnu4HI GCNGC 1 cut(s) 37
FokI GGATG 2 cut(s) 432, 460
Fsp4HI GCNGC 1 cut(s) 37
GluI GCNGC 1 cut(s) 37
GsaI CCCAGC 1 cut(s) 231
GsuI CTGGAG 1 cut(s) 93
HaeIII GGCC 1 cut(s) 329
Hin1II CATG 3 cut(s) 43, 316, 415
HinfI GANTC 2 cut(s) 26, 125
Hpy166II GTNNAC 2 cut(s) 65, 294
Hpy188I TCNGA 5 cut(s) 31, 130, 195, 450, 470
Hpy188III TCNNGA 3 cut(s) 72, 155, 266
Hpy8I GTNNAC 2 cut(s) 65, 294
HpyCH4III ACNGT 1 cut(s) 261
HpyCH4IV ACGT 1 cut(s) 20
HpyCH4V TGCA 1 cut(s) 36
HpyF3I CTNAG 3 cut(s) 201, 274, 467
HpySE526I ACGT 1 cut(s) 20
Hsp92II CATG 3 cut(s) 43, 316, 415
Kzo9I GATC 2 cut(s) 130, 385
LmnI GCTCC 1 cut(s) 85
Lsp1109I GCAGC 1 cut(s) 48
LweI GCATC 1 cut(s) 454
MaeII ACGT 1 cut(s) 20
MalI GATC 2 cut(s) 132, 387
MboI GATC 2 cut(s) 130, 385
MboII GAAGA 3 cut(s) 311, 382, 395
MfeI CAATTG 1 cut(s) 45
MhlI GDGCHC 1 cut(s) 460
MluCI AATT 4 cut(s) 45, 135, 220, 338
MnlI CCTC 4 cut(s) 179, 196, 275, 427
MseI TTAA 2 cut(s) 287, 479
MslI CAYNNNNRTG 3 cut(s) 144, 240, 434
MspR9I CCNGG 2 cut(s) 10, 253
MunI CAATTG 1 cut(s) 45
MvaI CCWGG 2 cut(s) 10, 253
NdeII GATC 2 cut(s) 130, 385
NlaIII CATG 3 cut(s) 43, 316, 415
NlaIV GGNNCC 2 cut(s) 52, 459
PfeI GAWTC 2 cut(s) 26, 125
PkrI GCNGC 1 cut(s) 38
Psp6I CCWGG 2 cut(s) 8, 251
PspFI CCCAGC 1 cut(s) 227
PspGI CCWGG 2 cut(s) 8, 251
PspN4I GGNNCC 2 cut(s) 52, 459
RsaI GTAC 2 cut(s) 19, 209
RsaNI GTAC 2 cut(s) 18, 208
RseI CAYNNNNRTG 3 cut(s) 144, 240, 434
SaqAI TTAA 2 cut(s) 287, 479
SatI GCNGC 1 cut(s) 37
Sau3AI GATC 2 cut(s) 130, 385
ScaI AGTACT 1 cut(s) 209
ScrFI CCNGG 2 cut(s) 10, 253
SduI GDGCHC 1 cut(s) 460
SetI ASST 4 cut(s) 23, 178, 190, 419
SfaNI GCATC 1 cut(s) 454
SmiMI CAYNNNNRTG 3 cut(s) 144, 240, 434
Sse9I AATT 4 cut(s) 45, 135, 220, 338
StyD4I CCNGG 2 cut(s) 8, 251
TaaI ACNGT 1 cut(s) 261
TaiI ACGT 1 cut(s) 23
TasI AATT 4 cut(s) 45, 135, 220, 338
TatI WGTACW 1 cut(s) 207
TfiI GAWTC 2 cut(s) 26, 125
Tru1I TTAA 2 cut(s) 287, 479
Tru9I TTAA 2 cut(s) 287, 479
TseI GCWGC 1 cut(s) 36
TspDTI ATGAA 1 cut(s) 128
TspGWI ACGGA 2 cut(s) 198, 415
XapI RAATTY 2 cut(s) 135, 220
XcmI CCANNNNNNNNNTGG 1 cut(s) 418
ZrmI AGTACT 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.