Rorug07G0101500

Haloacid dehalogenase-like hydrolase domain-containing protein Sgpp

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0006690
Physical Location & Seq
Forward (+)
8011541 .. 8012860
1320 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0101500.1

Sequence Viewer

Length: 1320 bp
ATGAGTCTCGCCGTCGATTCTCCGATTACCGACTCATCCGCCAGCAGCGATCCCGTCGATGACTTCGCCGGCTTTCTCGACGAAGTACTCGATTCCGATTCCGACGATGACGTGGCAAGTTTGCAGAGCACAAAACGGCGTAAGGTGGAGGTGAATAGTAATGGGTCGTCTTCAATTTCGAGCTCGGAAGTCTTGGAAGTAGAGAAGAATGGTTCTTTGTGTGAACATCTGGCTGGTTCTTACAAAGATATGTGCTTGATTTGTGGGCAAAGGTTGGATCAGAATGAGAATGAGAAATCCGAGTTGGAGGAGTTGAGGGTTAACAGTGTTCGTGAAATTGACCCAGGAAAACGAAATTCAAAGACTAGGGAGTTGCTGCTAGGACACAAGAAACTTCATTTGGTGCTTGATCTGGATCACACATTGCTCAATACTACTGCGGTGGGGTACTTGACGCCGCAGGAACGGTACTTGTTAGAGGGAGATCACTCTCTGGAAGATGTTTTTGTGGTGCGAAATTCGCCTGTGGATTTGGTGACCAAGTTGAGGCCGTTTGTTGGGACGTTTTTGAAGGAGGCCAGTGAGATGTTTGAGATGTCTGTGTACACAATGGGGGATCGACCCTATGCGCAGCAGATGGTTAAGCTGCTGGATCCTCGAAATGAGTACTTTGGTGATAGAGTTGTATCGAGAGATGATAGCACCAGGAAATTCGAAAAGAATTTGGATGTTGTGTGTGCTAAAGAGAGTGCTGTTTTGGTGCTTGATGATACACAGCAAGTGTGGATAAAGCAGAATCAGGACAATGTGGTACTAATGCGGAGGTATCTGTTCTTTAGGAACAGTTATGACTCTTTTGGCTTCAAGTATTGTATGTCTCATTCGGAGTTGAAGACTGATGAAAGTGATGAGCAGACTCAAGGAGCTTATCTAGCAGCTATTCTTCAGCTTCTTAAGCTTATCCACAGCATGTTCTTCGATGATAAAGTGGCTGCGCGGGACAATCTAATTGATAGAGATGTGAGGCAAGTGTTAAAAAGTGTTAGGAGTCAAGTCTTGAAAGGGTGTAAAATCGCCTTCGGCTGTGTTTTGGCATCAGAAGCCCAAGCTGATTTGTGGAACATGGCAGAAAAGTTGGGAGCAGTTTGTTCCACACAAGTAATGGATCCATCAGTCACACATGTGATCGAAACAGATGCGGAAACAGAAAATTCTCAATGGGCAGTGAAAGACAGCAAGTTTCTGGTCCATCCACAGTGGATTCAGGCTGCAAGTTTTATGTGGCAAAAGCAACCTGAAGACATGTTCTCTATATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003850 GO:0004346 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019203 GO:0042578 GO:0044237 GO:0050308 GO:0050309
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2

Protein Analysis

439

Amino Acids

49.67

Weight (kDa)

4.84

Isoelectric Point (pI)

39.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 133 - 284 2.9e-27 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 630
AccII CGCG 1 cut(s) 997
AciI CCGC 6 cut(s) 39, 440, 458, 820, 997, 1199
AclWI GGATC 8 cut(s) 44, 285, 423, 624, 647, 660, 1160, 1173
AcsI RAATTY 5 cut(s) 355, 517, 710, 721, 1210
AcuI CTGAAG 2 cut(s) 929, 1317
AcyI GRCGYC 1 cut(s) 455
AfaI GTAC 6 cut(s) 87, 449, 470, 605, 668, 813
AfiI CCNNNNNNNGG 2 cut(s) 546, 557
AflII CTTAAG 1 cut(s) 953
AflIII ACRYGT 2 cut(s) 1180, 1302
AgsI TTSAA 6 cut(s) 174, 360, 571, 865, 892, 1060
AjiI CACGTC 1 cut(s) 112
AjnI CCWGG 2 cut(s) 343, 704
AjuI GAANNNNNNNTTGG 4 cut(s) 287, 319, 383, 415
AleI CACNNNNGTG 1 cut(s) 1181
AluBI AGCT 7 cut(s) 183, 646, 926, 938, 949, 958, 1109
AluI AGCT 7 cut(s) 183, 646, 926, 938, 949, 958, 1109
Alw21I GWGCWC 2 cut(s) 131, 185
Alw26I GTCTC 2 cut(s) 11, 882
AlwI GGATC 8 cut(s) 44, 285, 423, 624, 647, 660, 1160, 1173
AoxI GGCC 2 cut(s) 548, 576
ApeKI GCWGC 7 cut(s) 45, 376, 631, 646, 935, 992, 1268
ApoI RAATTY 5 cut(s) 355, 517, 710, 721, 1210
AspLEI GCGC 2 cut(s) 631, 997
AspS9I GGNCC 1 cut(s) 1246
AsuHPI GGTGA 3 cut(s) 163, 547, 686
AsuII TTCGAA 1 cut(s) 714
AvaII GGWCC 1 cut(s) 1246
BamHI GGATCC 2 cut(s) 652, 1165
BanII GRGCYC 1 cut(s) 185
BbsI GAAGAC 3 cut(s) 162, 899, 1305
Bbv12I GWGCWC 2 cut(s) 131, 185
BbvI GCAGC 7 cut(s) 57, 363, 633, 643, 947, 979, 1255
BccI CCATC 3 cut(s) 631, 1177, 1257
BceAI ACGGC 2 cut(s) 152, 535
BcgI CGANNNNNNTGC 4 cut(s) 958, 992, 1178, 1212
BciT130I CCWGG 2 cut(s) 345, 706
BcoDI GTCTC 2 cut(s) 11, 882
BfaI CTAG 3 cut(s) 366, 380, 932
BfrI CTTAAG 1 cut(s) 953
BisI GCNGC 8 cut(s) 46, 377, 458, 632, 647, 936, 993, 1269
BlsI GCNGC 8 cut(s) 47, 378, 459, 633, 648, 937, 994, 1270
BmcAI AGTACT 2 cut(s) 87, 668
Bme1390I CCNGG 2 cut(s) 345, 706
Bme18I GGWCC 1 cut(s) 1246
BmgBI CACGTC 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 1246
BmiI GGNNCC 2 cut(s) 654, 1167
BmrFI CCNGG 2 cut(s) 345, 706
BmsI GCATC 2 cut(s) 1103, 1186
BpiI GAAGAC 3 cut(s) 162, 899, 1305
Bpu14I TTCGAA 1 cut(s) 714
BpuEI CTTGAG 1 cut(s) 903
BsaBI GATNNNNATC 1 cut(s) 414
BsaHI GRCGYC 1 cut(s) 455
BsaJI CCNNGG 1 cut(s) 343
Bsc4I CCNNNNNNNGG 2 cut(s) 546, 557
Bse118I RCCGGY 1 cut(s) 68
Bse1I ACTGG 1 cut(s) 579
Bse3DI GCAATG 1 cut(s) 422
Bse8I GATNNNNATC 1 cut(s) 414
BseBI CCWGG 2 cut(s) 345, 706
BseDI CCNNGG 1 cut(s) 343
BseGI GGATG 3 cut(s) 35, 733, 1249
BseJI GATNNNNATC 1 cut(s) 414
BseLI CCNNNNNNNGG 2 cut(s) 546, 557
BseMI GCAATG 1 cut(s) 422
BseNI ACTGG 1 cut(s) 579
BseRI GAGGAG 1 cut(s) 323
BseXI GCAGC 7 cut(s) 57, 363, 633, 643, 947, 979, 1255
Bsh1236I CGCG 1 cut(s) 997
BshFI GGCC 2 cut(s) 550, 578
BsiHKAI GWGCWC 2 cut(s) 131, 185
BsiSI CCGG 1 cut(s) 69
BslFI GGGAC 2 cut(s) 574, 1013
BslI CCNNNNNNNGG 2 cut(s) 546, 557
BsmAI GTCTC 2 cut(s) 11, 882
BsmFI GGGAC 2 cut(s) 574, 1013
BsnI GGCC 2 cut(s) 550, 578
Bsp119I TTCGAA 1 cut(s) 714
Bsp1286I GDGCHC 2 cut(s) 131, 185
Bsp1407I TGTACA 1 cut(s) 603
Bsp143I GATC 9 cut(s) 49, 277, 409, 415, 484, 616, 652, 1165, 1185
BspACI CCGC 6 cut(s) 39, 440, 458, 820, 997, 1199
BspANI GGCC 2 cut(s) 550, 578
BspFNI CGCG 1 cut(s) 997
BspLI GGNNCC 2 cut(s) 654, 1167
BspPI GGATC 8 cut(s) 44, 285, 423, 624, 647, 660, 1160, 1173
BspT104I TTCGAA 1 cut(s) 714
BspTI CTTAAG 1 cut(s) 953
BsrDI GCAATG 1 cut(s) 422
BsrFI RCCGGY 1 cut(s) 68
BsrGI TGTACA 1 cut(s) 603
BsrI ACTGG 1 cut(s) 579
BssAI RCCGGY 1 cut(s) 68
BssECI CCNNGG 1 cut(s) 343
BssMI GATC 9 cut(s) 49, 277, 409, 415, 484, 616, 652, 1165, 1185
BssNI GRCGYC 1 cut(s) 455
Bst2UI CCWGG 2 cut(s) 345, 706
Bst4CI ACNGT 4 cut(s) 326, 468, 845, 1257
BstACI GRCGYC 1 cut(s) 455
BstAFI CTTAAG 1 cut(s) 953
BstAUI TGTACA 1 cut(s) 603
BstBI TTCGAA 1 cut(s) 714
BstC8I GCNNGC 2 cut(s) 43, 70
BstEII GGTNACC 1 cut(s) 535
BstF5I GGATG 3 cut(s) 35, 733, 1249
BstFNI CGCG 1 cut(s) 997
BstHHI GCGC 2 cut(s) 631, 997
BstKTI GATC 9 cut(s) 52, 280, 412, 418, 487, 619, 655, 1168, 1188
BstMAI GTCTC 2 cut(s) 11, 882
BstMBI GATC 9 cut(s) 49, 277, 409, 415, 484, 616, 652, 1165, 1185
BstMWI GCNNNNNNNGC 4 cut(s) 520, 932, 955, 1100
BstNI CCWGG 2 cut(s) 345, 706
BstNSI RCATGY 3 cut(s) 973, 1184, 1306
BstPI GGTNACC 1 cut(s) 535
BstSCI CCNGG 2 cut(s) 343, 704
BstUI CGCG 1 cut(s) 997
BstV1I GCAGC 7 cut(s) 57, 363, 633, 643, 947, 979, 1255
BstV2I GAAGAC 3 cut(s) 162, 899, 1305
BstX2I RGATCY 2 cut(s) 652, 1165
BstYI RGATCY 2 cut(s) 652, 1165
BsuRI GGCC 2 cut(s) 550, 578
BtrI CACGTC 1 cut(s) 112
BtsCI GGATG 3 cut(s) 35, 733, 1249
BtsI GCAGTG 1 cut(s) 1230
BtsIMutI CAGTG 4 cut(s) 331, 586, 1230, 1262
Cac8I GCNNGC 2 cut(s) 43, 70
CfoI GCGC 2 cut(s) 631, 997
Cfr10I RCCGGY 1 cut(s) 68
Cfr13I GGNCC 1 cut(s) 1246
CseI GACGC 1 cut(s) 463
Csp6I GTAC 6 cut(s) 86, 448, 469, 604, 667, 812
CviAII CATG 4 cut(s) 970, 1123, 1181, 1303
CviQI GTAC 6 cut(s) 86, 448, 469, 604, 667, 812
DpnI GATC 9 cut(s) 51, 279, 411, 417, 486, 618, 654, 1167, 1187
DpnII GATC 9 cut(s) 49, 277, 409, 415, 484, 616, 652, 1165, 1185
EciI GGCGGA 1 cut(s) 28
Ecl136II GAGCTC 1 cut(s) 183
Eco24I GRGCYC 1 cut(s) 185
Eco47I GGWCC 1 cut(s) 1246
Eco53kI GAGCTC 1 cut(s) 183
Eco57I CTGAAG 2 cut(s) 929, 1317
Eco91I GGTNACC 1 cut(s) 535
EcoICRI GAGCTC 1 cut(s) 183
EcoO65I GGTNACC 1 cut(s) 535
EcoRII CCWGG 2 cut(s) 343, 704
EcoT38I GRGCYC 1 cut(s) 185
FaeI CATG 4 cut(s) 973, 1126, 1184, 1306
FaqI GGGAC 2 cut(s) 574, 1013
FatI CATG 4 cut(s) 969, 1122, 1180, 1302
FauI CCCGC 1 cut(s) 990
Fnu4HI GCNGC 8 cut(s) 46, 377, 458, 632, 647, 936, 993, 1269
FokI GGATG 3 cut(s) 22, 740, 1236
FriOI GRGCYC 1 cut(s) 185
Fsp4HI GCNGC 8 cut(s) 46, 377, 458, 632, 647, 936, 993, 1269
FspBI CTAG 3 cut(s) 366, 380, 932
FspI TGCGCA 1 cut(s) 630
GlaI GCGC 2 cut(s) 630, 996
GluI GCNGC 8 cut(s) 46, 377, 458, 632, 647, 936, 993, 1269
HaeIII GGCC 2 cut(s) 550, 578
HapII CCGG 1 cut(s) 69
HgaI GACGC 1 cut(s) 463
HhaI GCGC 2 cut(s) 631, 997
Hin1I GRCGYC 1 cut(s) 455
Hin1II CATG 4 cut(s) 973, 1126, 1184, 1306
Hin6I GCGC 2 cut(s) 629, 995
HinP1I GCGC 2 cut(s) 629, 995
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HindIII AAGCTT 1 cut(s) 956
HpaI GTTAAC 1 cut(s) 322
HpaII CCGG 1 cut(s) 69
HphI GGTGA 3 cut(s) 163, 547, 686
Hpy166II GTNNAC 4 cut(s) 224, 322, 604, 606
Hpy188I TCNGA 8 cut(s) 24, 97, 103, 187, 282, 301, 886, 1099
Hpy188III TCNNGA 7 cut(s) 77, 332, 413, 494, 690, 800, 1057
Hpy8I GTNNAC 4 cut(s) 224, 322, 604, 606
Hpy99I CGWCG 4 cut(s) 17, 59, 83, 107
HpyAV CCTTC 2 cut(s) 565, 1087
HpyCH4III ACNGT 4 cut(s) 326, 468, 845, 1257
HpyCH4IV ACGT 2 cut(s) 111, 563
HpyCH4V TGCA 2 cut(s) 124, 1271
HpyF10VI GCNNNNNNNGC 4 cut(s) 520, 932, 955, 1100
HpySE526I ACGT 2 cut(s) 111, 563
Hsp92I GRCGYC 1 cut(s) 455
Hsp92II CATG 4 cut(s) 973, 1126, 1184, 1306
HspAI GCGC 2 cut(s) 629, 995
KroI GCCGGC 1 cut(s) 68
KroNI GCCGGC 1 cut(s) 70
KspAI GTTAAC 1 cut(s) 322
Kzo9I GATC 9 cut(s) 49, 277, 409, 415, 484, 616, 652, 1165, 1185
LmnI GCTCC 2 cut(s) 923, 1139
Lsp1109I GCAGC 7 cut(s) 57, 363, 633, 643, 947, 979, 1255
LweI GCATC 2 cut(s) 1103, 1186
MaeI CTAG 3 cut(s) 366, 380, 932
MaeII ACGT 2 cut(s) 111, 563
MaeIII GTNAC 2 cut(s) 535, 1174
MalI GATC 9 cut(s) 51, 279, 411, 417, 486, 618, 654, 1167, 1187
MboI GATC 9 cut(s) 49, 277, 409, 415, 484, 616, 652, 1165, 1185
MboII GAAGA 7 cut(s) 162, 217, 509, 904, 935, 967, 1310
MflI RGATCY 2 cut(s) 652, 1165
MhlI GDGCHC 2 cut(s) 131, 185
MluCI AATT 8 cut(s) 174, 336, 355, 517, 710, 721, 1008, 1210
MlyI GAGTC 5 cut(s) 13, 26, 845, 910, 1057
MmeI TCCRAC 3 cut(s) 126, 255, 285
MnlI CCTC 9 cut(s) 142, 301, 309, 472, 540, 568, 666, 816, 1017
MroNI GCCGGC 1 cut(s) 68
MseI TTAA 4 cut(s) 321, 642, 954, 1034
MslI CAYNNNNRTG 1 cut(s) 1181
MspCI CTTAAG 1 cut(s) 953
MspI CCGG 1 cut(s) 69
MspR9I CCNGG 2 cut(s) 345, 706
MvaI CCWGG 2 cut(s) 345, 706
MvnI CGCG 1 cut(s) 997
MwoI GCNNNNNNNGC 4 cut(s) 520, 932, 955, 1100
NaeI GCCGGC 1 cut(s) 70
NdeII GATC 9 cut(s) 49, 277, 409, 415, 484, 616, 652, 1165, 1185
NgoMIV GCCGGC 1 cut(s) 68
NlaIII CATG 4 cut(s) 973, 1126, 1184, 1306
NlaIV GGNNCC 2 cut(s) 654, 1167
NmuCI GTSAC 2 cut(s) 535, 1174
NsbI TGCGCA 1 cut(s) 630
NspI RCATGY 3 cut(s) 973, 1184, 1306
NspV TTCGAA 1 cut(s) 714
OliI CACNNNNGTG 1 cut(s) 1181
PciI ACATGT 2 cut(s) 1180, 1302
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PdiI GCCGGC 1 cut(s) 70
PfeI GAWTC 5 cut(s) 17, 92, 98, 796, 1261
PkrI GCNGC 8 cut(s) 47, 378, 459, 633, 648, 937, 994, 1270
PleI GAGTC 5 cut(s) 12, 26, 845, 910, 1056
PpsI GAGTC 5 cut(s) 12, 26, 845, 910, 1056
PscI ACATGT 2 cut(s) 1180, 1302
Psp124BI GAGCTC 1 cut(s) 185
Psp6I CCWGG 2 cut(s) 343, 704
PspEI GGTNACC 1 cut(s) 535
PspGI CCWGG 2 cut(s) 343, 704
PspN4I GGNNCC 2 cut(s) 654, 1167
PspPI GGNCC 1 cut(s) 1246
PsuI RGATCY 2 cut(s) 652, 1165
RsaI GTAC 6 cut(s) 87, 449, 470, 605, 668, 813
RsaNI GTAC 6 cut(s) 86, 448, 469, 604, 667, 812
RseI CAYNNNNRTG 1 cut(s) 1181
SacI GAGCTC 1 cut(s) 185
SaqAI TTAA 4 cut(s) 321, 642, 954, 1034
SatI GCNGC 8 cut(s) 46, 377, 458, 632, 647, 936, 993, 1269
Sau3AI GATC 9 cut(s) 49, 277, 409, 415, 484, 616, 652, 1165, 1185
Sau96I GGNCC 1 cut(s) 1246
ScaI AGTACT 2 cut(s) 87, 668
SchI GAGTC 5 cut(s) 13, 26, 845, 910, 1057
ScrFI CCNGG 2 cut(s) 345, 706
SduI GDGCHC 2 cut(s) 131, 185
SfaNI GCATC 2 cut(s) 1103, 1186
SfuI TTCGAA 1 cut(s) 714
SinI GGWCC 1 cut(s) 1246
SmiMI CAYNNNNRTG 1 cut(s) 1181
SmlI CTYRAG 2 cut(s) 918, 953
SmoI CTYRAG 2 cut(s) 918, 953
Sse9I AATT 8 cut(s) 174, 336, 355, 517, 710, 721, 1008, 1210
SsiI CCGC 6 cut(s) 39, 440, 458, 820, 997, 1199
SspMI CTAG 3 cut(s) 366, 380, 932
SstI GAGCTC 1 cut(s) 185
StyD4I CCNGG 2 cut(s) 343, 704
TaaI ACNGT 4 cut(s) 326, 468, 845, 1257
TaiI ACGT 2 cut(s) 114, 566
TasI AATT 8 cut(s) 174, 336, 355, 517, 710, 721, 1008, 1210
TatI WGTACW 3 cut(s) 85, 603, 666
TauI GCSGC 1 cut(s) 460
TfiI GAWTC 5 cut(s) 17, 92, 98, 796, 1261
Tru1I TTAA 4 cut(s) 321, 642, 954, 1034
Tru9I TTAA 4 cut(s) 321, 642, 954, 1034
TscAI CASTG 4 cut(s) 331, 586, 1230, 1262
TseFI GTSAC 2 cut(s) 535, 1174
TseI GCWGC 7 cut(s) 45, 376, 631, 646, 935, 992, 1268
Tsp45I GTSAC 2 cut(s) 535, 1174
TspDTI ATGAA 2 cut(s) 386, 915
TspRI CASTG 4 cut(s) 331, 586, 1230, 1262
Vha464I CTTAAG 1 cut(s) 953
VpaK11BI GGWCC 1 cut(s) 1246
XapI RAATTY 5 cut(s) 355, 517, 710, 721, 1210
XceI RCATGY 3 cut(s) 973, 1184, 1306
XcmI CCANNNNNNNNNTGG 1 cut(s) 1159
XspI CTAG 3 cut(s) 366, 380, 932
ZrmI AGTACT 2 cut(s) 87, 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.