Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006690

Physical Location & Seq

Genomic Coordinates Reverse (-)

30321030 .. 30330298

Gene Structure 9269 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1227700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 618 bp

ATGGGTATTATGGAGGACCCTGTGCGCTCAGTGGTGACTTTGGCGATAAAGATGGAGCTCCTGCTGATTTCAAGCCTTCTTTCGGGACTGTTCTGTTCAGTGATATGTTCTGATCCTAAACTCGATTCACACCGTTTCAATGATATTCTTAATGAGGCAATAACTGCAGGTGCTGGAACCAGCAAAAGTTCACTCTCTGGAGTTGCTCCACTTGAAGCAGTAATATTTGATATTGATGGAACGCTGTGTGATTCGGATCCAATTCATTACTATGCCTTCCGTGAAATGCTACAAGAGATAGGTTTCAACGGAGGTGTTCCGATTACTGAGGAGTACTATATTGAGAATTTCGCTGGGAAGCATAATGATGATGTTGCCAGGATACTCTTTCCTGATGACTTCGAAAGAGGCTTAAAGTTCACAGAAGACAAGGAAGCCATGTTTCGCAGATTGGCTGTATATAAATTGAAGCCCGTGAATGGTCTCTATAAAGTGAAGAAATGGATTGAAGATCGTGGGTTGAAATGGAGAAAATCCATGACCTCATTGGAACTCATACTGTTCCCGCACCTGATATGGGCACCAATGCTCAGACCATGCTTAAATATCTATTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003850 GO:0004346 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019203 GO:0042578 GO:0044237 GO:0050308 GO:0050309

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HAD_2

Protein Analysis

206

Amino Acids

23.27

Weight (kDa)

5.43

Isoelectric Point (pI)

44.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydrolase	PF00702	72 - 151	3.8e-06	haloacid dehalogenase-like hydrolase
HAD_2	PF13419	74 - 175	3.9e-07	Haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006690)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38740
fragaria_vesca	FvH4_5g21050 FvH4_5g21050 FvH4_5g21050 FvH4_5g21050 FvH4_5g21050
malus_domestica	MD06G1041200.v1.1 MD12G1227700.v1.1 MD15G1033000.v1.1
prunus_persica	Prupe.5G047500_v2.0.a1 Prupe.5G047600_v2.0.a1 Prupe.5G047600_v2.0.a1
pyrus_communis	pycom06g03420
rosa_chinensis	RchiOBHm_Chr7g0208111
rosa_laevigata	RLG00000003254
rosa_multiflora	Rmu_sc0000706.1_g000042
rosa_roxburghii	Rroxscaffold_3G00250810
rosa_rugosa	Rorug07G0101400 Rorug07G0101500 Rorug07G0101600 Rorug07G0101700
rosa_samantha	Rh7AG234500 Rh7BG231100 Rh7CG250700 Rh7DG241500
rosa_wichuraiana	Rw7G020080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	158
Acc36I	ACCTGC	1 cut(s)	158
AccB1I	GGYRCC	1 cut(s)	580
AciI	CCGC	1 cut(s)	566
AclWI	GGATC	3 cut(s)	107, 251, 264
AcsI	RAATTY	1 cut(s)	346
AfaI	GTAC	1 cut(s)	335
AfiI	CCNNNNNNNGG	3 cut(s)	82, 479, 577
AgsI	TTSAA	7 cut(s)	72, 139, 215, 307, 469, 509, 523
AjnI	CCWGG	1 cut(s)	377
AluBI	AGCT	1 cut(s)	58
AluI	AGCT	1 cut(s)	58
Alw21I	GWGCWC	1 cut(s)	60
Alw26I	GTCTC	1 cut(s)	488
AlwI	GGATC	3 cut(s)	107, 251, 264
AlwNI	CAGNNNCTG	1 cut(s)	173
ApoI	RAATTY	1 cut(s)	346
AspLEI	GCGC	1 cut(s)	27
AspS9I	GGNCC	1 cut(s)	16
AsuHPI	GGTGA	1 cut(s)	46
AsuII	TTCGAA	1 cut(s)	402
AvaII	GGWCC	1 cut(s)	16
BaeGI	GKGCMC	1 cut(s)	583
BamHI	GGATCC	1 cut(s)	256
BanI	GGYRCC	1 cut(s)	580
BanII	GRGCYC	1 cut(s)	60
BbsI	GAAGAC	1 cut(s)	432
Bbv12I	GWGCWC	1 cut(s)	60
BccI	CCATC	2 cut(s)	46, 230
BciT130I	CCWGG	1 cut(s)	379
BciVI	GTATCC	1 cut(s)	375
BcoDI	GTCTC	1 cut(s)	488
BfmI	CTRYAG	1 cut(s)	165
BfuAI	ACCTGC	1 cut(s)	158
BfuI	GTATCC	1 cut(s)	375
BmcAI	AGTACT	1 cut(s)	335
Bme1390I	CCNGG	1 cut(s)	379
Bme18I	GGWCC	1 cut(s)	16
BmgT120I	GGNCC	1 cut(s)	16
BmiI	GGNNCC	4 cut(s)	18, 178, 258, 582
BmrFI	CCNGG	1 cut(s)	379
BpiI	GAAGAC	1 cut(s)	432
BpmI	CTGGAG	1 cut(s)	219
Bpu14I	TTCGAA	1 cut(s)	402
BsaBI	GATNNNNATC	1 cut(s)	255
BsaI	GGTCTC	1 cut(s)	488
Bsc4I	CCNNNNNNNGG	3 cut(s)	82, 479, 577
Bse8I	GATNNNNATC	1 cut(s)	255
BseBI	CCWGG	1 cut(s)	379
BseJI	GATNNNNATC	1 cut(s)	255
BseLI	CCNNNNNNNGG	3 cut(s)	82, 479, 577
BseMII	CTCAG	3 cut(s)	42, 318, 604
BseRI	GAGGAG	1 cut(s)	344
BseSI	GKGCMC	1 cut(s)	583
BseYI	CCCAGC	1 cut(s)	353
BshNI	GGYRCC	1 cut(s)	580
BsiHKAI	GWGCWC	1 cut(s)	60
BslFI	GGGAC	1 cut(s)	99
BslI	CCNNNNNNNGG	3 cut(s)	82, 479, 577
BsmAI	GTCTC	1 cut(s)	488
BsmFI	GGGAC	1 cut(s)	99
Bso31I	GGTCTC	1 cut(s)	488
Bsp119I	TTCGAA	1 cut(s)	402
Bsp1286I	GDGCHC	2 cut(s)	60, 583
Bsp143I	GATC	3 cut(s)	112, 256, 511
BspACI	CCGC	1 cut(s)	566
BspCNI	CTCAG	3 cut(s)	41, 319, 603
BspLI	GGNNCC	4 cut(s)	18, 178, 258, 582
BspMAI	CTGCAG	1 cut(s)	169
BspMI	ACCTGC	1 cut(s)	158
BspPI	GGATC	3 cut(s)	107, 251, 264
BspT104I	TTCGAA	1 cut(s)	402
BspT107I	GGYRCC	1 cut(s)	580
BspTNI	GGTCTC	1 cut(s)	488
BssMI	GATC	3 cut(s)	112, 256, 511
Bst2UI	CCWGG	1 cut(s)	379
Bst4CI	ACNGT	3 cut(s)	90, 134, 561
BstAPI	GCANNNNNTGC	1 cut(s)	164
BstBI	TTCGAA	1 cut(s)	402
BstDEI	CTNAG	3 cut(s)	28, 327, 590
BstHHI	GCGC	1 cut(s)	27
BstKTI	GATC	3 cut(s)	115, 259, 514
BstMAI	GTCTC	1 cut(s)	488
BstMBI	GATC	3 cut(s)	112, 256, 511
BstMWI	GCNNNNNNNGC	1 cut(s)	164
BstNI	CCWGG	1 cut(s)	379
BstSCI	CCNGG	1 cut(s)	377
BstSFI	CTRYAG	1 cut(s)	165
BstSLI	GKGCMC	1 cut(s)	583
BstV2I	GAAGAC	1 cut(s)	432
BstX2I	RGATCY	1 cut(s)	256
BstYI	RGATCY	1 cut(s)	256
BsuI	GTATCC	1 cut(s)	375
BtsIMutI	CAGTG	2 cut(s)	36, 105
BveI	ACCTGC	1 cut(s)	158
CaiI	CAGNNNCTG	1 cut(s)	173
CfoI	GCGC	1 cut(s)	27
Cfr13I	GGNCC	1 cut(s)	16
Csp6I	GTAC	1 cut(s)	334
CviAII	CATG	3 cut(s)	439, 538, 597
CviJI	RGCY	6 cut(s)	58, 75, 411, 437, 455, 472
CviKI_1	RGCY	6 cut(s)	58, 75, 411, 437, 455, 472
CviQI	GTAC	1 cut(s)	334
DdeI	CTNAG	3 cut(s)	28, 327, 590
DpnI	GATC	3 cut(s)	114, 258, 513
DpnII	GATC	3 cut(s)	112, 256, 511
Ecl136II	GAGCTC	1 cut(s)	58
Eco24I	GRGCYC	1 cut(s)	60
Eco31I	GGTCTC	1 cut(s)	488
Eco47I	GGWCC	1 cut(s)	16
Eco53kI	GAGCTC	1 cut(s)	58
EcoICRI	GAGCTC	1 cut(s)	58
EcoO109I	RGGNCCY	1 cut(s)	16
EcoRII	CCWGG	1 cut(s)	377
EcoT38I	GRGCYC	1 cut(s)	60
FaeI	CATG	3 cut(s)	442, 541, 600
FaqI	GGGAC	1 cut(s)	99
FatI	CATG	3 cut(s)	438, 537, 596
FauI	CCCGC	1 cut(s)	573
FriOI	GRGCYC	1 cut(s)	60
GlaI	GCGC	1 cut(s)	26
GsaI	CCCAGC	1 cut(s)	357
GsuI	CTGGAG	1 cut(s)	219
HhaI	GCGC	1 cut(s)	27
Hin1II	CATG	3 cut(s)	442, 541, 600
Hin6I	GCGC	1 cut(s)	25
HinP1I	GCGC	1 cut(s)	25
HinfI	GANTC	2 cut(s)	125, 251
HphI	GGTGA	1 cut(s)	46
Hpy166II	GTNNAC	2 cut(s)	191, 420
Hpy188I	TCNGA	4 cut(s)	112, 256, 321, 593
Hpy188III	TCNNGA	3 cut(s)	84, 198, 392
Hpy8I	GTNNAC	2 cut(s)	191, 420
HpyAV	CCTTC	2 cut(s)	86, 286
HpyCH4III	ACNGT	3 cut(s)	90, 134, 561
HpyCH4V	TGCA	1 cut(s)	167
HpyF10VI	GCNNNNNNNGC	1 cut(s)	164
HpyF3I	CTNAG	3 cut(s)	28, 327, 590
Hsp92II	CATG	3 cut(s)	442, 541, 600
HspAI	GCGC	1 cut(s)	25
Kzo9I	GATC	3 cut(s)	112, 256, 511
LmnI	GCTCC	3 cut(s)	55, 63, 211
MaeIII	GTNAC	1 cut(s)	34
MalI	GATC	3 cut(s)	114, 258, 513
MboI	GATC	3 cut(s)	112, 256, 511
MboII	GAAGA	3 cut(s)	437, 508, 521
MflI	RGATCY	1 cut(s)	256
MhlI	GDGCHC	2 cut(s)	60, 583
MluCI	AATT	3 cut(s)	261, 346, 464
MnlI	CCTC	6 cut(s)	7, 148, 305, 322, 401, 553
MseI	TTAA	3 cut(s)	150, 413, 602
MslI	CAYNNNNRTG	2 cut(s)	270, 366
MspR9I	CCNGG	1 cut(s)	379
MvaI	CCWGG	1 cut(s)	379
MwoI	GCNNNNNNNGC	1 cut(s)	164
NdeII	GATC	3 cut(s)	112, 256, 511
NlaIII	CATG	3 cut(s)	442, 541, 600
NlaIV	GGNNCC	4 cut(s)	18, 178, 258, 582
NmuCI	GTSAC	1 cut(s)	34
NspV	TTCGAA	1 cut(s)	402
PaqCI	CACCTGC	1 cut(s)	158
PfeI	GAWTC	2 cut(s)	125, 251
PpuMI	RGGWCCY	1 cut(s)	16
Psp124BI	GAGCTC	1 cut(s)	60
Psp5II	RGGWCCY	1 cut(s)	16
Psp6I	CCWGG	1 cut(s)	377
PspFI	CCCAGC	1 cut(s)	353
PspGI	CCWGG	1 cut(s)	377
PspN4I	GGNNCC	4 cut(s)	18, 178, 258, 582
PspPI	GGNCC	1 cut(s)	16
PspPPI	RGGWCCY	1 cut(s)	16
PstI	CTGCAG	1 cut(s)	169
PstNI	CAGNNNCTG	1 cut(s)	173
PsuI	RGATCY	1 cut(s)	256
RsaI	GTAC	1 cut(s)	335
RsaNI	GTAC	1 cut(s)	334
RseI	CAYNNNNRTG	2 cut(s)	270, 366
SacI	GAGCTC	1 cut(s)	60
SaqAI	TTAA	3 cut(s)	150, 413, 602
Sau3AI	GATC	3 cut(s)	112, 256, 511
Sau96I	GGNCC	1 cut(s)	16
ScaI	AGTACT	1 cut(s)	335
ScrFI	CCNGG	1 cut(s)	379
SduI	GDGCHC	2 cut(s)	60, 583
SetI	ASST	6 cut(s)	60, 172, 304, 316, 545, 573
SfcI	CTRYAG	1 cut(s)	165
SfuI	TTCGAA	1 cut(s)	402
SinI	GGWCC	1 cut(s)	16
SmiMI	CAYNNNNRTG	2 cut(s)	270, 366
Sse9I	AATT	3 cut(s)	261, 346, 464
SsiI	CCGC	1 cut(s)	566
SspI	AATATT	1 cut(s)	225
SstI	GAGCTC	1 cut(s)	60
StyD4I	CCNGG	1 cut(s)	377
TaaI	ACNGT	3 cut(s)	90, 134, 561
TaqI	TCGA	2 cut(s)	123, 402
TasI	AATT	3 cut(s)	261, 346, 464
TatI	WGTACW	1 cut(s)	333
TfiI	GAWTC	2 cut(s)	125, 251
Tru1I	TTAA	3 cut(s)	150, 413, 602
Tru9I	TTAA	3 cut(s)	150, 413, 602
TscAI	CASTG	2 cut(s)	36, 105
TseFI	GTSAC	1 cut(s)	34
Tsp45I	GTSAC	1 cut(s)	34
TspDTI	ATGAA	1 cut(s)	254
TspGWI	ACGGA	2 cut(s)	269, 324
TspRI	CASTG	2 cut(s)	36, 105
VpaK11BI	GGWCC	1 cut(s)	16
XapI	RAATTY	1 cut(s)	346
XcmI	CCANNNNNNNNNTGG	1 cut(s)	544
ZrmI	AGTACT	1 cut(s)	335

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.