MD06G1069100.v1.1

YT521-B-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0002140
Physical Location & Seq
Reverse (-)
16609737 .. 16612809
3073 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1069100.v1.1.491

Sequence Viewer

Length: 789 bp
ATGAATGAACCAATTTTGGAAGAAGCCTTTCATAACTCTGGTAAAGTAATTTTAATATTCAGTGTCAACATGAGTGGTGCCTTCCAAGGGTATGCTCAAATGATATCTTCTGTTGGATGGAGACGAGACAATGTATGGAGTCAAGGAAGTAGTAGAAGTAATCCATGGGGACGAAGTTTTAAGGTCAAATGGCTTCGGCTAAATGATCTTCCTTTCCAAAAGACTCTTCATCTCAAAAATCCATTGAATGAGTACAAACCCGTTAAAATTAGCAGAGACTGCCAGGAGTTGTCTCCAGATGTTGGAGAAGTTCTTTGTGAGCTTCTTGATGGGACGGATGATGTGCACGGCTTACCGAACGGCAGTATTCCCATGGATGATTTTCCTTCAAAAAGGCCTTGTTTAGAGCCTCCATGTTCATTAGGGGAGGAAGAGTATAATGTGGGTCCCATGCATCTATCGTGGTCCCAGACACCCATGATGTATCCTTCGTTTCTTTACCAGCAGCAGGCTGAAGCAAATAGATATCCTTTAGCAAATCAGAGATCTACTGAAAGTCGTCTAGCTAGCTTTCTTCTGACTGAAGATGATTTTTTGGAAATGTCTTATGAAGAATACCTGGAGGCCCAAAGCAGAAGTTGCAAGGAATTATGTCTGCCTGGTTCTAGATCAGAGGAATCATCAAGAAGTAGAATGCAGGAACATGATTCAAACTTAAGCTATGCAAGCTACCGCAGCTGCTCAAGGAAAAGGCCTCATCATTCATCCCACAGGCAATCACAATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YTH

Protein Analysis

263

Amino Acids

30.13

Weight (kDa)

5.79

Isoelectric Point (pI)

72.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YTH PF04146 2 - 109 1.9e-36 YT521-B-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 77
AccB7I CCANNNNNTGG 1 cut(s) 302
AciI CCGC 1 cut(s) 733
AcuI CTGAAG 2 cut(s) 534, 603
AfaI GTAC 1 cut(s) 254
AfiI CCNNNNNNNGG 3 cut(s) 87, 302, 508
AflII CTTAAG 1 cut(s) 715
AgsI TTSAA 3 cut(s) 247, 390, 711
AjnI CCWGG 3 cut(s) 282, 618, 658
AjuI GAANNNNNNNTTGG 2 cut(s) 210, 242
AluBI AGCT 6 cut(s) 322, 566, 570, 720, 729, 738
AluI AGCT 6 cut(s) 322, 566, 570, 720, 729, 738
Alw21I GWGCWC 1 cut(s) 348
Alw26I GTCTC 4 cut(s) 115, 120, 270, 297
Alw44I GTGCAC 1 cut(s) 344
AlwNI CAGNNNCTG 1 cut(s) 279
AoxI GGCC 3 cut(s) 395, 624, 752
ApaLI GTGCAC 1 cut(s) 344
ApeKI GCWGC 3 cut(s) 505, 735, 738
Asp700I GAANNNNTTC 1 cut(s) 27
AspS9I GGNCC 3 cut(s) 446, 465, 625
AsuNHI GCTAGC 1 cut(s) 566
AvaII GGWCC 2 cut(s) 446, 465
BaeGI GKGCMC 1 cut(s) 348
BanI GGYRCC 1 cut(s) 77
Bbv12I GWGCWC 1 cut(s) 348
BbvI GCAGC 3 cut(s) 517, 725, 747
BccI CCATC 2 cut(s) 111, 323
BceAI ACGGC 2 cut(s) 364, 376
BciT130I CCWGG 3 cut(s) 284, 620, 660
BciVI GTATCC 1 cut(s) 495
BcoDI GTCTC 4 cut(s) 115, 120, 270, 297
BfaI CTAG 3 cut(s) 563, 567, 666
BfrI CTTAAG 1 cut(s) 715
BfuI GTATCC 1 cut(s) 495
BglII AGATCT 1 cut(s) 545
BisI GCNGC 3 cut(s) 506, 736, 739
BlsI GCNGC 3 cut(s) 507, 737, 740
Bme1390I CCNGG 3 cut(s) 284, 620, 660
Bme18I GGWCC 2 cut(s) 446, 465
BmgT120I GGNCC 3 cut(s) 446, 465, 625
BmiI GGNNCC 4 cut(s) 79, 447, 448, 467
BmrFI CCNGG 3 cut(s) 284, 620, 660
BmsI GCATC 1 cut(s) 463
BmtI GCTAGC 1 cut(s) 570
BpmI CTGGAG 2 cut(s) 279, 641
BpuEI CTTGAG 1 cut(s) 727
BsaJI CCNNGG 3 cut(s) 85, 164, 372
BsaXI ACNNNNNCTCC 2 cut(s) 419, 449
Bsc4I CCNNNNNNNGG 3 cut(s) 87, 302, 508
BseBI CCWGG 3 cut(s) 284, 620, 660
BseDI CCNNGG 3 cut(s) 85, 164, 372
BseGI GGATG 4 cut(s) 122, 343, 382, 764
BseLI CCNNNNNNNGG 3 cut(s) 87, 302, 508
BseSI GKGCMC 1 cut(s) 348
BseXI GCAGC 3 cut(s) 517, 725, 747
BshFI GGCC 3 cut(s) 397, 626, 754
BshNI GGYRCC 1 cut(s) 77
BsiHKAI GWGCWC 1 cut(s) 348
BslFI GGGAC 4 cut(s) 183, 346, 432, 451
BslI CCNNNNNNNGG 3 cut(s) 87, 302, 508
BsmAI GTCTC 4 cut(s) 115, 120, 270, 297
BsmBI CGTCTC 1 cut(s) 115
BsmFI GGGAC 4 cut(s) 183, 346, 432, 451
BsmI GAATGC 1 cut(s) 699
BsnI GGCC 3 cut(s) 397, 626, 754
Bsp1286I GDGCHC 1 cut(s) 348
Bsp143I GATC 3 cut(s) 205, 545, 668
Bsp19I CCATGG 2 cut(s) 164, 372
BspACI CCGC 1 cut(s) 733
BspANI GGCC 3 cut(s) 397, 626, 754
BspLI GGNNCC 4 cut(s) 79, 447, 448, 467
BspOI GCTAGC 1 cut(s) 570
BspT107I GGYRCC 1 cut(s) 77
BspTI CTTAAG 1 cut(s) 715
BssECI CCNNGG 3 cut(s) 85, 164, 372
BssMI GATC 3 cut(s) 205, 545, 668
BssT1I CCWWGG 3 cut(s) 85, 164, 372
Bst2UI CCWGG 3 cut(s) 284, 620, 660
Bst6I CTCTTC 2 cut(s) 231, 426
BstAFI CTTAAG 1 cut(s) 715
BstAPI GCANNNNNTGC 2 cut(s) 279, 639
BstC8I GCNNGC 3 cut(s) 510, 568, 727
BstDSI CCRYGG 2 cut(s) 164, 372
BstF5I GGATG 4 cut(s) 122, 343, 382, 764
BstKTI GATC 3 cut(s) 208, 548, 671
BstMAI GTCTC 4 cut(s) 115, 120, 270, 297
BstMBI GATC 3 cut(s) 205, 545, 668
BstMWI GCNNNNNNNGC 4 cut(s) 279, 639, 726, 735
BstNI CCWGG 3 cut(s) 284, 620, 660
BstSCI CCNGG 3 cut(s) 282, 618, 658
BstSLI GKGCMC 1 cut(s) 348
BstV1I GCAGC 3 cut(s) 517, 725, 747
BstX2I RGATCY 1 cut(s) 545
BstYI RGATCY 1 cut(s) 545
BsuI GTATCC 1 cut(s) 495
BsuRI GGCC 3 cut(s) 397, 626, 754
BtgI CCRYGG 2 cut(s) 164, 372
BtsCI GGATG 4 cut(s) 122, 343, 382, 764
BtsIMutI CAGTG 1 cut(s) 67
Cac8I GCNNGC 3 cut(s) 510, 568, 727
CaiI CAGNNNCTG 1 cut(s) 279
Cfr13I GGNCC 3 cut(s) 446, 465, 625
Csp6I GTAC 1 cut(s) 253
CspCI CAANNNNNGTGG 2 cut(s) 55, 90
CviAII CATG 7 cut(s) 70, 165, 373, 414, 451, 478, 704
CviQI GTAC 1 cut(s) 253
DpnI GATC 3 cut(s) 207, 547, 670
DpnII GATC 3 cut(s) 205, 545, 668
Eam1104I CTCTTC 2 cut(s) 231, 426
EarI CTCTTC 2 cut(s) 231, 426
Eco130I CCWWGG 3 cut(s) 85, 164, 372
Eco147I AGGCCT 2 cut(s) 397, 754
Eco32I GATATC 2 cut(s) 105, 527
Eco47I GGWCC 2 cut(s) 446, 465
Eco57I CTGAAG 2 cut(s) 534, 603
EcoO109I RGGNCCY 1 cut(s) 446
EcoRII CCWGG 3 cut(s) 282, 618, 658
EcoRV GATATC 2 cut(s) 105, 527
EcoT14I CCWWGG 3 cut(s) 85, 164, 372
EcoT22I ATGCAT 1 cut(s) 456
ErhI CCWWGG 3 cut(s) 85, 164, 372
Esp3I CGTCTC 1 cut(s) 115
FaeI CATG 7 cut(s) 73, 168, 376, 417, 454, 481, 707
FaqI GGGAC 4 cut(s) 183, 346, 432, 451
FatI CATG 7 cut(s) 69, 164, 372, 413, 450, 477, 703
Fnu4HI GCNGC 3 cut(s) 506, 736, 739
FokI GGATG 4 cut(s) 129, 350, 389, 751
Fsp4HI GCNGC 3 cut(s) 506, 736, 739
FspBI CTAG 3 cut(s) 563, 567, 666
GluI GCNGC 3 cut(s) 506, 736, 739
GsuI CTGGAG 2 cut(s) 279, 641
HaeIII GGCC 3 cut(s) 397, 626, 754
Hin1II CATG 7 cut(s) 73, 168, 376, 417, 454, 481, 707
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HinfI GANTC 4 cut(s) 139, 223, 677, 707
Hpy166II GTNNAC 2 cut(s) 67, 346
Hpy188I TCNGA 3 cut(s) 543, 579, 673
Hpy188III TCNNGA 4 cut(s) 296, 326, 666, 684
Hpy8I GTNNAC 2 cut(s) 67, 346
HpyAV CCTTC 3 cut(s) 91, 396, 498
HpyCH4V TGCA 5 cut(s) 346, 454, 642, 697, 725
HpyF10VI GCNNNNNNNGC 4 cut(s) 279, 639, 726, 735
Hsp92II CATG 7 cut(s) 73, 168, 376, 417, 454, 481, 707
KflI GGGWCCC 1 cut(s) 446
Kzo9I GATC 3 cut(s) 205, 545, 668
Lsp1109I GCAGC 3 cut(s) 517, 725, 747
LweI GCATC 1 cut(s) 463
MaeI CTAG 3 cut(s) 563, 567, 666
MalI GATC 3 cut(s) 207, 547, 670
MboI GATC 3 cut(s) 205, 545, 668
MboII GAAGA 8 cut(s) 32, 99, 200, 218, 443, 566, 596, 623
MflI RGATCY 1 cut(s) 545
MhlI GDGCHC 1 cut(s) 348
MluCI AATT 4 cut(s) 12, 48, 267, 647
MlyI GAGTC 2 cut(s) 148, 217
MmeI TCCRAC 2 cut(s) 94, 283
MnlI CCTC 5 cut(s) 420, 421, 616, 667, 765
Mph1103I ATGCAT 1 cut(s) 456
MroXI GAANNNNTTC 1 cut(s) 27
MseI TTAA 4 cut(s) 53, 180, 264, 716
MspA1I CMGCKG 1 cut(s) 738
MspCI CTTAAG 1 cut(s) 715
MspR9I CCNGG 3 cut(s) 284, 620, 660
Mva1269I GAATGC 1 cut(s) 699
MvaI CCWGG 3 cut(s) 284, 620, 660
MwoI GCNNNNNNNGC 4 cut(s) 279, 639, 726, 735
NcoI CCATGG 2 cut(s) 164, 372
NdeII GATC 3 cut(s) 205, 545, 668
NheI GCTAGC 1 cut(s) 566
NlaIII CATG 7 cut(s) 73, 168, 376, 417, 454, 481, 707
NlaIV GGNNCC 4 cut(s) 79, 447, 448, 467
NsiI ATGCAT 1 cut(s) 456
PceI AGGCCT 2 cut(s) 397, 754
PctI GAATGC 1 cut(s) 699
PdmI GAANNNNTTC 1 cut(s) 27
PfeI GAWTC 2 cut(s) 677, 707
PflMI CCANNNNNTGG 1 cut(s) 302
PkrI GCNGC 3 cut(s) 507, 737, 740
PleI GAGTC 2 cut(s) 147, 217
PpsI GAGTC 2 cut(s) 147, 217
PpuMI RGGWCCY 1 cut(s) 446
Psp5II RGGWCCY 1 cut(s) 446
Psp6I CCWGG 3 cut(s) 282, 618, 658
PspGI CCWGG 3 cut(s) 282, 618, 658
PspN4I GGNNCC 4 cut(s) 79, 447, 448, 467
PspPI GGNCC 3 cut(s) 446, 465, 625
PspPPI RGGWCCY 1 cut(s) 446
PstNI CAGNNNCTG 1 cut(s) 279
PsuI RGATCY 1 cut(s) 545
PvuII CAGCTG 1 cut(s) 738
RsaI GTAC 1 cut(s) 254
RsaNI GTAC 1 cut(s) 253
SaqAI TTAA 4 cut(s) 53, 180, 264, 716
SatI GCNGC 3 cut(s) 506, 736, 739
Sau3AI GATC 3 cut(s) 205, 545, 668
Sau96I GGNCC 3 cut(s) 446, 465, 625
SchI GAGTC 2 cut(s) 148, 217
ScrFI CCNGG 3 cut(s) 284, 620, 660
SduI GDGCHC 1 cut(s) 348
SetI ASST 8 cut(s) 186, 324, 568, 572, 621, 722, 731, 740
SfaNI GCATC 1 cut(s) 463
SinI GGWCC 2 cut(s) 446, 465
SmlI CTYRAG 2 cut(s) 715, 742
SmoI CTYRAG 2 cut(s) 715, 742
Sse9I AATT 4 cut(s) 12, 48, 267, 647
SseBI AGGCCT 2 cut(s) 397, 754
SsiI CCGC 1 cut(s) 733
SspI AATATT 1 cut(s) 57
SspMI CTAG 3 cut(s) 563, 567, 666
StuI AGGCCT 2 cut(s) 397, 754
StyD4I CCNGG 3 cut(s) 282, 618, 658
StyI CCWWGG 3 cut(s) 85, 164, 372
TasI AATT 4 cut(s) 12, 48, 267, 647
TatI WGTACW 1 cut(s) 252
TfiI GAWTC 2 cut(s) 677, 707
Tru1I TTAA 4 cut(s) 53, 180, 264, 716
Tru9I TTAA 4 cut(s) 53, 180, 264, 716
TscAI CASTG 1 cut(s) 67
TseI GCWGC 3 cut(s) 505, 735, 738
TspDTI ATGAA 7 cut(s) 17, 20, 21, 218, 408, 624, 753
TspGWI ACGGA 1 cut(s) 350
TspRI CASTG 1 cut(s) 67
Van91I CCANNNNNTGG 1 cut(s) 302
Vha464I CTTAAG 1 cut(s) 715
VneI GTGCAC 1 cut(s) 344
VpaK11BI GGWCC 2 cut(s) 446, 465
XbaI TCTAGA 1 cut(s) 665
XmnI GAANNNNTTC 1 cut(s) 27
XspI CTAG 3 cut(s) 563, 567, 666
Zsp2I ATGCAT 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.