Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0005626

Physical Location & Seq

Genomic Coordinates Forward (+)

20387139 .. 20393995

Gene Structure 6857 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1082900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1833 bp

ATGAGGAACCGGCGGTTTGCGCAGGTCACGACGAGCGACGACGAAGACGACGACGTCGTACCCCGGAGCCACCAGCAGTCGGCGGGGGATACATCGTCGCGGAAGAGGAAGCAGGTGAAGCTTCGAGAAGGGGACGACGAGGAGTCGGGGGAGGAGGAGAAGCAGAAGAGTAAGAAGAAGAGGAGAGGGAAGGAGAAAGAGGCCGAAACGGCGTCGGGTAGCGAAGACGAGGAGGAGGAGCCACACGTGGTGGAGGACGCGAAGCCGATTGGCGAGGTCGTTAAGCTTTCCGGGAAGGGAAGGGGCCGGAGGCAGCACTACAAGGCGTTTGAGTACGACGGGACCCGATTTGACCTGGAGGATCCGGTTCTTCTAACTCCTGAGGATACAAAACAAAAGCCTTATGTGGCAATTATCAAGGATATAAGTAGGACCGGAGGTGGGAGCATGATGGTATTGGGACAGTGGTTTTATCGTCCTGAAGAGGCAGAGAAAAAAGCTGGTGGAAACTGGCAGTCAACTGATACAAGGGAGCTGTTCTATAGTTTCCATCGTGATGAGGTTCCTGCAGAATCTGTAATGCATAAATGTTTGGTGCATTTTGTTCCGTTAAACAAACAGCTTCCAAGTCGTAAACAGCATCCTGGCTTCATTGTGCAAAAGGTGTATGACACCCAAGGGAAAAAACTCTGGAAGCTGACAGATAGAGACTACGAAGAGGATAAGCAACATGAGATTGACTTGCTAGTTCAGAAAACTATTAAACTATTGGGAGAACTTCCTGATATAGAGATTGCAGATAACAATGCTTATCAGGATGATCAGTTGAAGACTAAAAGGGGTCTGATTAAAAAGAATATATCACCCCTTGATGTTTCTAGGGTGGAAGAAGCAACTGCTAGGCCTGGTCACAATCAAAAAGCTGAAACGCCTGGAAGCTGCCCAACAAGCAACTCAGAATATCATTCCATTTTAGCAAAGTCCATGGCACTAACTGGAGACACACTACGTGACAGATGGTTGGAGAAACTTCTACAAAATATCCGGCACCACGTATGGAACTCTGCTGACACTACAAACGGTATTGAAAAGAGAAAATCTGGCTCTGATGATATTGATCAAGAAAGTGACCGTAAGTCCACAGAAGTTGGAAATGGATCTCAGGATAATCTCAAGAGTGGCAACACGTCTTTTCTCTGGCCAGAGGATGCTGTTCGTGCAGTTACTTCTCTAGAGAAGGCCTCGCATGAGTCTCTCTCCTCAGATTTCCAAAAGTATAACCAGAAGTTGCGGCAACTGGGATTCAATCTCCAGAAAAATTCATTCCTAGCCCGTCGTCTGCTAAATCGAGAGTTGGAACCTTTAACAATAGTGAACATGTCACCTGAGGAGTTAAAGGAGGGTTTAACAGCTGAGGAGAGAGCAAAGAAGGAGCCTGATGATTGGGAGCGTATGCAGATGACTGACGCTCGTTGCTCAAGATGCTCAGAGTTCAAAGTGGGAGTTAGGGACATTATTCAAGCAGGACACAGTGCTCGCTATCAGCTGGAGTGTATTGCCTGTGGTCATTCATGGTATGCTGCCCGGGATGCGGTTTCAATGCTCACAATTGATGCACCCAGTTCTAACAAGAATGTGGGGACGGCTCCTTTGGCCACTGCCAAGTTTGAAGATGTTGAAAAGAAGCTGGCGAGCCCACGTGAGCCTGACAAGGCAACCAAGGACACTCTAAGAAAAACAACTGAAGCATATATGCCGGTGTTGGAAGCGCAAAGATCACTTAGCAAGTCCAAAAAGGAAGAAAATTCTGATTCTGCAAAGAAAGCCGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BAH DUF3527 TFIIS_M

Protein Analysis

611

Amino Acids

69.25

Weight (kDa)

6.28

Isoelectric Point (pI)

54.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BAH	PF01426	119 - 233	3.6e-20	BAH domain
TFIIS_M	PF07500	402 - 481	3.5e-14	Transcription factor S-II (TFIIS), central domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005626)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11560 AT4G11560
fragaria_vesca	FvH4_4g24221 FvH4_4g36830 FvH4_5g02390 FvH4_5g02390 FvH4_5g02390 FvH4_5g02390 FvH4_5g02390
malus_domestica	MD06G1082900.v1.1 MD14G1104300.v1.1
prunus_persica	Prupe.5G100000_v2.0.a1 Prupe.5G100000_v2.0.a1
pyrus_communis	pycom06g07970 pycom06g07980 pycom14g10050
rosa_chinensis	RchiOBHm_Chr7g0197721
rosa_laevigata	RLG00000003917 RLG00000015451
rosa_multiflora	Rmu_sc0006732.1_g000010 Rmu_sc0029951.1_g000001 Rmu_sc0029952.1_g000001
rosa_roxburghii	Rroxscaffold_3G00258270
rosa_rugosa	Rorug07G0042600
rosa_samantha	Rh7AG166900 Rh7BG169000 Rh7CG175400 Rh7DG168700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	103
AasI	GACNNNNNNGTC	1 cut(s)	53
AatII	GACGTC	1 cut(s)	57
Acc16I	TGCGCA	1 cut(s)	21
Acc36I	ACCTGC	2 cut(s)	13, 103
AccB1I	GGYRCC	1 cut(s)	1047
AccII	CGCG	2 cut(s)	100, 260
AciI	CCGC	5 cut(s)	13, 83, 100, 1291, 1592
AclWI	GGATC	3 cut(s)	356, 369, 1165
AcoI	YGGCCR	2 cut(s)	1199, 1653
AcsI	RAATTY	2 cut(s)	1318, 1804
AcuI	CTGAAG	2 cut(s)	501, 1764
AcvI	CACGTG	2 cut(s)	247, 1700
AcyI	GRCGYC	2 cut(s)	54, 212
AdeI	CACNNNGTG	2 cut(s)	250, 1010
AfaI	GTAC	2 cut(s)	60, 335
AfiI	CCNNNNNNNGG	3 cut(s)	79, 441, 1591
AflIII	ACRYGT	3 cut(s)	244, 1185, 1377
AgsI	TTSAA	8 cut(s)	829, 1088, 1306, 1495, 1520, 1599, 1670, 1679
AhdI	GACNNNNNGTC	2 cut(s)	142, 1135
AjiI	CACGTC	1 cut(s)	1188
AjnI	CCWGG	4 cut(s)	354, 643, 904, 931
Alw21I	GWGCWC	1 cut(s)	1537
Alw26I	GTCTC	3 cut(s)	702, 993, 1257
AlwI	GGATC	3 cut(s)	356, 369, 1165
AlwNI	CAGNNNCTG	1 cut(s)	575
Ama87I	CYCGRG	1 cut(s)	1584
AoxI	GGCC	6 cut(s)	201, 304, 902, 1199, 1239, 1653
ApeKI	GCWGC	3 cut(s)	313, 939, 1580
ApoI	RAATTY	2 cut(s)	1318, 1804
ArsI	GACNNNNNNTTYG	2 cut(s)	1633, 1665
AspLEI	GCGC	2 cut(s)	22, 1771
AspS9I	GGNCC	3 cut(s)	304, 342, 432
AsuC2I	CCSGG	4 cut(s)	64, 292, 1585, 1586
AsuHPI	GGTGA	3 cut(s)	127, 855, 1374
AvaI	CYCGRG	1 cut(s)	1584
AvaII	GGWCC	2 cut(s)	342, 432
AxyI	CCTNAGG	2 cut(s)	381, 1386
BalI	TGGCCA	2 cut(s)	1201, 1655
BamHI	GGATCC	1 cut(s)	361
BanI	GGYRCC	1 cut(s)	1047
BanII	GRGCYC	1 cut(s)	1697
BbrPI	CACGTG	2 cut(s)	247, 1700
BbsI	GAAGAC	3 cut(s)	51, 231, 836
Bbv12I	GWGCWC	1 cut(s)	1537
BbvCI	CCTCAGC	1 cut(s)	1413
BbvI	GCAGC	3 cut(s)	325, 926, 1567
BccI	CCATC	3 cut(s)	445, 558, 1011
BceAI	ACGGC	2 cut(s)	225, 1659
BciT130I	CCWGG	4 cut(s)	356, 645, 906, 933
BciVI	GTATCC	2 cut(s)	82, 379
BclI	TGATCA	2 cut(s)	820, 1117
BcnI	CCSGG	4 cut(s)	64, 292, 1585, 1586
BcoDI	GTCTC	3 cut(s)	702, 993, 1257
BfaI	CTAG	5 cut(s)	746, 879, 900, 1232, 1328
BfmI	CTRYAG	2 cut(s)	541, 567
BfuAI	ACCTGC	2 cut(s)	13, 103
BfuI	GTATCC	2 cut(s)	82, 379
BglI	GCCNNNNNGGC	1 cut(s)	209
BisI	GCNGC	4 cut(s)	314, 940, 1292, 1581
BlsI	GCNGC	4 cut(s)	315, 941, 1293, 1582
Bme1390I	CCNGG	8 cut(s)	64, 292, 356, 645, 906, 933, 1585, 1586
Bme18I	GGWCC	2 cut(s)	342, 432
BmeRI	GACNNNNNGTC	2 cut(s)	142, 1135
BmeT110I	CYCGRG	1 cut(s)	1584
BmgBI	CACGTC	1 cut(s)	1188
BmgT120I	GGNCC	3 cut(s)	304, 342, 432
BmrFI	CCNGG	8 cut(s)	64, 292, 356, 645, 906, 933, 1585, 1586
BmrI	ACTGGG	2 cut(s)	1307, 1614
BmsI	GCATC	5 cut(s)	649, 1198, 1472, 1579, 1603
BmuI	ACTGGG	2 cut(s)	1307, 1614
BpiI	GAAGAC	3 cut(s)	51, 231, 836
BplI	GAGNNNNNCTC	4 cut(s)	1226, 1258, 1241, 1273
BpmI	CTGGAG	4 cut(s)	377, 1017, 1295, 1568
Bpu10I	CCTNAGC	1 cut(s)	1413
BpuEI	CTTGAG	2 cut(s)	1157, 1462
BpuMI	CCSGG	4 cut(s)	64, 292, 1585, 1586
BsaAI	YACGTR	4 cut(s)	247, 1010, 1054, 1700
BsaBI	GATNNNNATC	1 cut(s)	1116
BsaHI	GRCGYC	2 cut(s)	54, 212
BsaJI	CCNNGG	5 cut(s)	62, 676, 984, 1584, 1719
BsaWI	WCCGGW	2 cut(s)	364, 434
Bsc4I	CCNNNNNNNGG	3 cut(s)	79, 441, 1591
Bse118I	RCCGGY	2 cut(s)	9, 1756
Bse1I	ACTGG	4 cut(s)	515, 1000, 1302, 1620
Bse21I	CCTNAGG	2 cut(s)	381, 1386
Bse8I	GATNNNNATC	1 cut(s)	1116
BseBI	CCWGG	4 cut(s)	356, 645, 906, 933
BseDI	CCNNGG	5 cut(s)	62, 676, 984, 1584, 1719
BseGI	GGATG	4 cut(s)	640, 823, 1213, 1594
BseJI	GATNNNNATC	1 cut(s)	1116
BseLI	CCNNNNNNNGG	3 cut(s)	79, 441, 1591
BseMII	CTCAG	7 cut(s)	372, 969, 1175, 1275, 1377, 1404, 1500
BseNI	ACTGG	4 cut(s)	515, 1000, 1302, 1620
BseXI	GCAGC	3 cut(s)	325, 926, 1567
BsgI	GTGCAG	1 cut(s)	1239
Bsh1236I	CGCG	2 cut(s)	100, 260
BshFI	GGCC	6 cut(s)	203, 306, 904, 1201, 1241, 1655
BshNI	GGYRCC	1 cut(s)	1047
BsiHKAI	GWGCWC	1 cut(s)	1537
BsiHKCI	CYCGRG	1 cut(s)	1584
BsiSI	CCGG	9 cut(s)	10, 64, 291, 307, 365, 435, 1045, 1585, 1757
BslFI	GGGAC	5 cut(s)	146, 355, 474, 1523, 1654
BslI	CCNNNNNNNGG	3 cut(s)	79, 441, 1591
BsmAI	GTCTC	3 cut(s)	702, 993, 1257
BsmFI	GGGAC	5 cut(s)	146, 355, 474, 1523, 1654
BsnI	GGCC	6 cut(s)	203, 306, 904, 1201, 1241, 1655
BsoBI	CYCGRG	1 cut(s)	1584
Bsp1286I	GDGCHC	2 cut(s)	1537, 1697
Bsp143I	GATC	5 cut(s)	361, 820, 1117, 1157, 1775
Bsp19I	CCATGG	1 cut(s)	984
BspACI	CCGC	5 cut(s)	13, 83, 100, 1291, 1592
BspANI	GGCC	6 cut(s)	203, 306, 904, 1201, 1241, 1655
BspCNI	CTCAG	7 cut(s)	373, 968, 1174, 1274, 1378, 1405, 1499
BspFNI	CGCG	2 cut(s)	100, 260
BspMAI	CTGCAG	1 cut(s)	571
BspMI	ACCTGC	2 cut(s)	13, 103
BspPI	GGATC	3 cut(s)	356, 369, 1165
BspT107I	GGYRCC	1 cut(s)	1047
BsrFI	RCCGGY	2 cut(s)	9, 1756
BsrI	ACTGG	4 cut(s)	515, 1000, 1302, 1620
BssAI	RCCGGY	2 cut(s)	9, 1756
BssECI	CCNNGG	5 cut(s)	62, 676, 984, 1584, 1719
BssMI	GATC	5 cut(s)	361, 820, 1117, 1157, 1775
BssNI	GRCGYC	2 cut(s)	54, 212
BssT1I	CCWWGG	3 cut(s)	676, 984, 1719
Bst2UI	CCWGG	4 cut(s)	356, 645, 906, 933
Bst4CI	ACNGT	4 cut(s)	465, 1082, 1133, 1532
Bst6I	CTCTTC	5 cut(s)	98, 161, 173, 477, 711
BstACI	GRCGYC	2 cut(s)	54, 212
BstBAI	YACGTR	4 cut(s)	247, 1010, 1054, 1700
BstC8I	GCNNGC	3 cut(s)	1537, 1689, 1693
BstDEI	CTNAG	9 cut(s)	381, 955, 1161, 1261, 1386, 1413, 1486, 1730, 1781
BstDSI	CCRYGG	1 cut(s)	984
BstF5I	GGATG	4 cut(s)	640, 823, 1213, 1594
BstFNI	CGCG	2 cut(s)	100, 260
BstHHI	GCGC	2 cut(s)	22, 1771
BstKTI	GATC	5 cut(s)	364, 823, 1120, 1160, 1778
BstMAI	GTCTC	3 cut(s)	702, 993, 1257
BstMBI	GATC	5 cut(s)	361, 820, 1117, 1157, 1775
BstMWI	GCNNNNNNNGC	9 cut(s)	19, 118, 209, 948, 1217, 1482, 1589, 1652, 1823
BstNI	CCWGG	4 cut(s)	356, 645, 906, 933
BstNSI	RCATGY	1 cut(s)	1381
BstSCI	CCNGG	8 cut(s)	62, 290, 354, 643, 904, 931, 1583, 1584
BstSFI	CTRYAG	2 cut(s)	541, 567
BstUI	CGCG	2 cut(s)	100, 260
BstV1I	GCAGC	3 cut(s)	325, 926, 1567
BstV2I	GAAGAC	3 cut(s)	51, 231, 836
BstX2I	RGATCY	2 cut(s)	361, 1157
BstYI	RGATCY	2 cut(s)	361, 1157
Bsu36I	CCTNAGG	2 cut(s)	381, 1386
BsuI	GTATCC	2 cut(s)	82, 379
BsuRI	GGCC	6 cut(s)	203, 306, 904, 1201, 1241, 1655
BtgI	CCRYGG	1 cut(s)	984
BtrI	CACGTC	1 cut(s)	1188
BtsCI	GGATG	4 cut(s)	640, 823, 1213, 1594
BtsI	GCAGTG	1 cut(s)	1656
BtsIMutI	CAGTG	3 cut(s)	470, 1537, 1656
BveI	ACCTGC	2 cut(s)	13, 103
Cac8I	GCNNGC	3 cut(s)	1537, 1689, 1693
CaiI	CAGNNNCTG	1 cut(s)	575
CfoI	GCGC	2 cut(s)	22, 1771
Cfr10I	RCCGGY	2 cut(s)	9, 1756
Cfr13I	GGNCC	3 cut(s)	304, 342, 432
Cfr9I	CCCGGG	1 cut(s)	1584
CseI	GACGC	3 cut(s)	201, 266, 1475
Csp6I	GTAC	2 cut(s)	59, 334
CspCI	CAANNNNNGTGG	2 cut(s)	1129, 1164
CviAII	CATG	6 cut(s)	448, 731, 985, 1247, 1378, 1572
CviQI	GTAC	2 cut(s)	59, 334
DdeI	CTNAG	9 cut(s)	381, 955, 1161, 1261, 1386, 1413, 1486, 1730, 1781
DpnI	GATC	5 cut(s)	363, 822, 1119, 1159, 1777
DpnII	GATC	5 cut(s)	361, 820, 1117, 1157, 1775
DraIII	CACNNNGTG	2 cut(s)	250, 1010
DrdI	GACNNNNNNGTC	1 cut(s)	53
DriI	GACNNNNNGTC	2 cut(s)	142, 1135
DseDI	GACNNNNNNGTC	1 cut(s)	53
EaeI	YGGCCR	2 cut(s)	1199, 1653
Eam1104I	CTCTTC	5 cut(s)	98, 161, 173, 477, 711
Eam1105I	GACNNNNNGTC	2 cut(s)	142, 1135
EarI	CTCTTC	5 cut(s)	98, 161, 173, 477, 711
Eco130I	CCWWGG	3 cut(s)	676, 984, 1719
Eco147I	AGGCCT	2 cut(s)	904, 1241
Eco24I	GRGCYC	1 cut(s)	1697
Eco47I	GGWCC	2 cut(s)	342, 432
Eco57I	CTGAAG	2 cut(s)	501, 1764
Eco72I	CACGTG	2 cut(s)	247, 1700
Eco81I	CCTNAGG	2 cut(s)	381, 1386
Eco88I	CYCGRG	1 cut(s)	1584
EcoO109I	RGGNCCY	1 cut(s)	342
EcoRII	CCWGG	4 cut(s)	354, 643, 904, 931
EcoT14I	CCWWGG	3 cut(s)	676, 984, 1719
EcoT22I	ATGCAT	1 cut(s)	585
EcoT38I	GRGCYC	1 cut(s)	1697
ErhI	CCWWGG	3 cut(s)	676, 984, 1719
FaeI	CATG	6 cut(s)	451, 734, 988, 1250, 1381, 1575
FaqI	GGGAC	5 cut(s)	146, 355, 474, 1523, 1654
FatI	CATG	6 cut(s)	447, 730, 984, 1246, 1377, 1571
FauI	CCCGC	1 cut(s)	76
FbaI	TGATCA	2 cut(s)	820, 1117
Fnu4HI	GCNGC	4 cut(s)	314, 940, 1292, 1581
FokI	GGATG	4 cut(s)	627, 830, 1220, 1601
FriOI	GRGCYC	1 cut(s)	1697
Fsp4HI	GCNGC	4 cut(s)	314, 940, 1292, 1581
FspBI	CTAG	5 cut(s)	746, 879, 900, 1232, 1328
FspI	TGCGCA	1 cut(s)	21
GlaI	GCGC	2 cut(s)	21, 1770
GluI	GCNGC	4 cut(s)	314, 940, 1292, 1581
GsuI	CTGGAG	4 cut(s)	377, 1017, 1295, 1568
HaeIII	GGCC	6 cut(s)	203, 306, 904, 1201, 1241, 1655
HapII	CCGG	9 cut(s)	10, 64, 291, 307, 365, 435, 1045, 1585, 1757
HgaI	GACGC	3 cut(s)	201, 266, 1475
HhaI	GCGC	2 cut(s)	22, 1771
Hin1I	GRCGYC	2 cut(s)	54, 212
Hin1II	CATG	6 cut(s)	451, 734, 988, 1250, 1381, 1575
Hin6I	GCGC	2 cut(s)	20, 1769
HinP1I	GCGC	2 cut(s)	20, 1769
HincII	GTYRAC	1 cut(s)	519
HindII	GTYRAC	1 cut(s)	519
HindIII	AAGCTT	2 cut(s)	119, 284
HinfI	GANTC	5 cut(s)	143, 572, 1250, 1302, 1811
HpaII	CCGG	9 cut(s)	10, 64, 291, 307, 365, 435, 1045, 1585, 1757
HphI	GGTGA	3 cut(s)	127, 855, 1374
Hpy166II	GTNNAC	4 cut(s)	519, 635, 1140, 1375
Hpy188I	TCNGA	7 cut(s)	753, 846, 958, 1108, 1264, 1489, 1810
Hpy8I	GTNNAC	4 cut(s)	519, 635, 1140, 1375
HpyAV	CCTTC	6 cut(s)	122, 184, 289, 294, 1231, 1423
HpyCH4III	ACNGT	4 cut(s)	465, 1082, 1133, 1532
HpyCH4IV	ACGT	6 cut(s)	54, 246, 1009, 1053, 1187, 1699
HpyCH4V	TGCA	9 cut(s)	569, 583, 598, 658, 797, 1220, 1456, 1616, 1817
HpyF10VI	GCNNNNNNNGC	9 cut(s)	19, 118, 209, 948, 1217, 1482, 1589, 1652, 1823
HpyF3I	CTNAG	9 cut(s)	381, 955, 1161, 1261, 1386, 1413, 1486, 1730, 1781
HpySE526I	ACGT	6 cut(s)	54, 246, 1009, 1053, 1187, 1699
Hsp92I	GRCGYC	2 cut(s)	54, 212
Hsp92II	CATG	6 cut(s)	451, 734, 988, 1250, 1381, 1575
HspAI	GCGC	2 cut(s)	20, 1769
KflI	GGGWCCC	1 cut(s)	342
Ksp22I	TGATCA	2 cut(s)	820, 1117
Kzo9I	GATC	5 cut(s)	361, 820, 1117, 1157, 1775
LmnI	GCTCC	7 cut(s)	66, 238, 444, 532, 1432, 1447, 1651
Lsp1109I	GCAGC	3 cut(s)	325, 926, 1567
LweI	GCATC	5 cut(s)	649, 1198, 1472, 1579, 1603
MaeI	CTAG	5 cut(s)	746, 879, 900, 1232, 1328
MaeII	ACGT	6 cut(s)	54, 246, 1009, 1053, 1187, 1699
MaeIII	GTNAC	6 cut(s)	25, 908, 1010, 1127, 1222, 1380
MalI	GATC	5 cut(s)	363, 822, 1119, 1159, 1777
MboI	GATC	5 cut(s)	361, 820, 1117, 1157, 1775
MfeI	CAATTG	1 cut(s)	1608
MflI	RGATCY	2 cut(s)	361, 1157
MhlI	GDGCHC	2 cut(s)	1537, 1697
MlsI	TGGCCA	2 cut(s)	1201, 1655
MluCI	AATT	4 cut(s)	411, 1318, 1608, 1804
MluNI	TGGCCA	2 cut(s)	1201, 1655
MlyI	GAGTC	2 cut(s)	152, 1259
MmeI	TCCRAC	4 cut(s)	1002, 1129, 1335, 1743
Mox20I	TGGCCA	2 cut(s)	1201, 1655
Mph1103I	ATGCAT	1 cut(s)	585
MscI	TGGCCA	2 cut(s)	1201, 1655
MseI	TTAA	7 cut(s)	282, 611, 762, 849, 1364, 1394, 1406
MslI	CAYNNNNRTG	1 cut(s)	555
Msp20I	TGGCCA	2 cut(s)	1201, 1655
MspA1I	CMGCKG	2 cut(s)	1412, 1546
MspI	CCGG	9 cut(s)	10, 64, 291, 307, 365, 435, 1045, 1585, 1757
MspR9I	CCNGG	8 cut(s)	64, 292, 356, 645, 906, 933, 1585, 1586
MunI	CAATTG	1 cut(s)	1608
MvaI	CCWGG	4 cut(s)	356, 645, 906, 933
MvnI	CGCG	2 cut(s)	100, 260
MwoI	GCNNNNNNNGC	9 cut(s)	19, 118, 209, 948, 1217, 1482, 1589, 1652, 1823
NciI	CCSGG	4 cut(s)	64, 292, 1585, 1586
NcoI	CCATGG	1 cut(s)	984
NdeII	GATC	5 cut(s)	361, 820, 1117, 1157, 1775
NlaIII	CATG	6 cut(s)	451, 734, 988, 1250, 1381, 1575
NmuCI	GTSAC	5 cut(s)	25, 908, 1010, 1127, 1380
NsbI	TGCGCA	1 cut(s)	21
NsiI	ATGCAT	1 cut(s)	585
NspI	RCATGY	1 cut(s)	1381
PaqCI	CACCTGC	1 cut(s)	103
PceI	AGGCCT	2 cut(s)	904, 1241
PciI	ACATGT	1 cut(s)	1377
PcsI	WCGNNNNNNNCGW	3 cut(s)	45, 48, 54
PfeI	GAWTC	3 cut(s)	572, 1302, 1811
PflFI	GACNNNGTC	1 cut(s)	53
PfoI	TCCNGGA	1 cut(s)	290
PkrI	GCNGC	4 cut(s)	315, 941, 1293, 1582
PleI	GAGTC	2 cut(s)	151, 1258
PmaCI	CACGTG	2 cut(s)	247, 1700
PmlI	CACGTG	2 cut(s)	247, 1700
PpsI	GAGTC	2 cut(s)	151, 1258
Ppu21I	YACGTR	4 cut(s)	247, 1010, 1054, 1700
PpuMI	RGGWCCY	1 cut(s)	342
PscI	ACATGT	1 cut(s)	1377
Psp5II	RGGWCCY	1 cut(s)	342
Psp6I	CCWGG	4 cut(s)	354, 643, 904, 931
PspCI	CACGTG	2 cut(s)	247, 1700
PspGI	CCWGG	4 cut(s)	354, 643, 904, 931
PspPI	GGNCC	3 cut(s)	304, 342, 432
PspPPI	RGGWCCY	1 cut(s)	342
PstI	CTGCAG	1 cut(s)	571
PstNI	CAGNNNCTG	1 cut(s)	575
PsuI	RGATCY	2 cut(s)	361, 1157
PsyI	GACNNNGTC	1 cut(s)	53
PvuII	CAGCTG	2 cut(s)	1412, 1546
RsaI	GTAC	2 cut(s)	60, 335
RsaNI	GTAC	2 cut(s)	59, 334
RseI	CAYNNNNRTG	1 cut(s)	555
SaqAI	TTAA	7 cut(s)	282, 611, 762, 849, 1364, 1394, 1406
SatI	GCNGC	4 cut(s)	314, 940, 1292, 1581
Sau3AI	GATC	5 cut(s)	361, 820, 1117, 1157, 1775
Sau96I	GGNCC	3 cut(s)	304, 342, 432
SchI	GAGTC	2 cut(s)	152, 1259
ScrFI	CCNGG	8 cut(s)	64, 292, 356, 645, 906, 933, 1585, 1586
SduI	GDGCHC	2 cut(s)	1537, 1697
SfaNI	GCATC	5 cut(s)	649, 1198, 1472, 1579, 1603
SfcI	CTRYAG	2 cut(s)	541, 567
SinI	GGWCC	2 cut(s)	342, 432
SmaI	CCCGGG	1 cut(s)	1586
SmiMI	CAYNNNNRTG	1 cut(s)	555
SmlI	CTYRAG	2 cut(s)	1172, 1477
SmoI	CTYRAG	2 cut(s)	1172, 1477
Sse9I	AATT	4 cut(s)	411, 1318, 1608, 1804
SseBI	AGGCCT	2 cut(s)	904, 1241
SsiI	CCGC	5 cut(s)	13, 83, 100, 1291, 1592
SspMI	CTAG	5 cut(s)	746, 879, 900, 1232, 1328
StuI	AGGCCT	2 cut(s)	904, 1241
StyD4I	CCNGG	8 cut(s)	62, 290, 354, 643, 904, 931, 1583, 1584
StyI	CCWWGG	3 cut(s)	676, 984, 1719
TaaI	ACNGT	4 cut(s)	465, 1082, 1133, 1532
TaiI	ACGT	6 cut(s)	57, 249, 1012, 1056, 1190, 1702
TaqI	TCGA	2 cut(s)	124, 1348
TasI	AATT	4 cut(s)	411, 1318, 1608, 1804
TauI	GCSGC	1 cut(s)	1294
TfiI	GAWTC	3 cut(s)	572, 1302, 1811
Tru1I	TTAA	7 cut(s)	282, 611, 762, 849, 1364, 1394, 1406
Tru9I	TTAA	7 cut(s)	282, 611, 762, 849, 1364, 1394, 1406
TscAI	CASTG	3 cut(s)	470, 1537, 1663
TseFI	GTSAC	5 cut(s)	25, 908, 1010, 1127, 1380
TseI	GCWGC	3 cut(s)	313, 939, 1580
Tsp45I	GTSAC	5 cut(s)	25, 908, 1010, 1127, 1380
TspDTI	ATGAA	3 cut(s)	640, 1311, 1560
TspGWI	ACGGA	1 cut(s)	597
TspMI	CCCGGG	1 cut(s)	1584
TspRI	CASTG	3 cut(s)	470, 1537, 1663
Tth111I	GACNNNGTC	1 cut(s)	53
VpaK11BI	GGWCC	2 cut(s)	342, 432
XapI	RAATTY	2 cut(s)	1318, 1804
XbaI	TCTAGA	1 cut(s)	1231
XceI	RCATGY	1 cut(s)	1381
XmaI	CCCGGG	1 cut(s)	1584
XspI	CTAG	5 cut(s)	746, 879, 900, 1232, 1328
ZraI	GACGTC	1 cut(s)	55
Zsp2I	ATGCAT	1 cut(s)	585

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.