pycom06g07970

Transcription factor S-II (TFIIS), central domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0005626
Physical Location & Seq
Forward (+)
13030229 .. 13033355
3127 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g07970.1

Sequence Viewer

Length: 951 bp
ATGCTACTGTCGTCCTCAGAGCTTCCCAGTCTGAGACACAGCAGATATAGAGAGAGAAGGGCCCTTGACAATCAGATTACCAAAACGCAGCGTTTCACCTGGAAAATCATGAGGAACCGGCGGTTTGCACAGGTCACGACGAGCGACGACGAAGACGACGACGTCGCACCCCGGAGCCACCAGCAGTCGGCGGGGGATACATCGTCGCGGAAGAGGAAGCAGGTGAAGCTTCGAGAAGGGGACGACGAAGAGTCGGGGGAGGAGGAGAAGCAGAAGAGTAAGAAGAAGAGGAGAGGGAAGGAGAAAGAGGCCGAAGCGGCGTCAGGTAGCGAAGACGAGGAGGAGGAGCCACACGTGGTGGAGGACGCAAAGCCGATTGGCGAGGTCGTTAAGCTTTCCGGGAAGGGAAGGGGCCGGAGGCAGCATTACAAGGCGTTTGAGTATGACGGGACCCGATTTGACCTGGAGGATCCGGTTCTTCTAACTCCTGAGGATACCAGACAAAAGCCTTATGTGGCAATTATCAAGGTACATATAAGCAGGACCGGAGGTGGGAGCATGATGGTATTAGGACAGTGGTTTTATCGTCCTGAAGAGGCAGAGAAAAAAGCTGGTGGAAACTGGCAGTCAACTGATACAAGGGAGTTGTTCTATAGTTTCCATCGTGATGAGGTTCCTGCAGAATCCGTAATGCATAAATGTTTGGTGCATTTTGTTCCGTTAAACAAACAGCTTCCAAGTCGTAAACAGCATCCTGGCTTCATTGTGCAAAAGGTGTATGACACCCAAGGGAAAAAACTCTGGAAGTTGACAGATAGAGACTACGAAGAGGATAAGCAACATGAGATTGACTTGCTAGTTCAGAAAACTATTAAACTATTGGGAGAACTTCCTGATATAGAGATTGCAGATAACAATGTTTATCAGGATGATCGATCAGTTGAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BAH DUF3527 TFIIS_M

Protein Analysis

317

Amino Acids

36.73

Weight (kDa)

6.26

Isoelectric Point (pI)

60.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BAH PF01426 155 - 270 8.4e-19 BAH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 211
AasI GACNNNNNNGTC 1 cut(s) 161
AatII GACGTC 1 cut(s) 165
Acc36I ACCTGC 1 cut(s) 211
AccII CGCG 1 cut(s) 208
AciI CCGC 4 cut(s) 121, 191, 208, 317
AclWI GGATC 2 cut(s) 464, 477
AcuI CTGAAG 1 cut(s) 612
AcvI CACGTG 1 cut(s) 355
AcyI GRCGYC 2 cut(s) 162, 320
AdeI CACNNNGTG 1 cut(s) 358
AfaI GTAC 1 cut(s) 531
AfiI CCNNNNNNNGG 2 cut(s) 187, 552
AflIII ACRYGT 1 cut(s) 352
AgsI TTSAA 1 cut(s) 944
AhdI GACNNNNNGTC 1 cut(s) 250
AjnI CCWGG 3 cut(s) 98, 462, 754
AluBI AGCT 5 cut(s) 22, 229, 394, 611, 733
AluI AGCT 5 cut(s) 22, 229, 394, 611, 733
Alw26I GTCTC 2 cut(s) 28, 813
AlwI GGATC 2 cut(s) 464, 477
AoxI GGCC 3 cut(s) 60, 309, 412
ApaI GGGCCC 1 cut(s) 64
ApeKI GCWGC 2 cut(s) 88, 421
AspS9I GGNCC 5 cut(s) 60, 61, 412, 450, 543
AsuC2I CCSGG 2 cut(s) 172, 400
AsuHPI GGTGA 2 cut(s) 88, 235
AvaII GGWCC 2 cut(s) 450, 543
AxyI CCTNAGG 1 cut(s) 489
BaeGI GKGCMC 1 cut(s) 64
BamHI GGATCC 1 cut(s) 469
BanII GRGCYC 1 cut(s) 64
BbrPI CACGTG 1 cut(s) 355
BbsI GAAGAC 2 cut(s) 159, 339
BbvI GCAGC 2 cut(s) 100, 433
BccI CCATC 2 cut(s) 556, 669
BciT130I CCWGG 3 cut(s) 100, 464, 756
BciVI GTATCC 2 cut(s) 190, 487
BcnI CCSGG 2 cut(s) 172, 400
BcoDI GTCTC 2 cut(s) 28, 813
BfaI CTAG 1 cut(s) 857
BfmI CTRYAG 2 cut(s) 652, 678
BfuAI ACCTGC 1 cut(s) 211
BfuI GTATCC 2 cut(s) 190, 487
BglI GCCNNNNNGGC 1 cut(s) 317
BisI GCNGC 3 cut(s) 89, 318, 422
BlsI GCNGC 3 cut(s) 90, 319, 423
Bme1390I CCNGG 5 cut(s) 100, 172, 400, 464, 756
Bme18I GGWCC 2 cut(s) 450, 543
BmeRI GACNNNNNGTC 1 cut(s) 250
BmgT120I GGNCC 5 cut(s) 60, 61, 412, 450, 543
BmiI GGNNCC 9 cut(s) 62, 116, 176, 348, 413, 451, 452, 471, 675
BmrFI CCNGG 5 cut(s) 100, 172, 400, 464, 756
BmrI ACTGGG 1 cut(s) 21
BmsI GCATC 1 cut(s) 760
BmuI ACTGGG 1 cut(s) 21
BpiI GAAGAC 2 cut(s) 159, 339
BpmI CTGGAG 1 cut(s) 485
BpuMI CCSGG 2 cut(s) 172, 400
Bsa29I ATCGAT 1 cut(s) 934
BsaAI YACGTR 1 cut(s) 355
BsaHI GRCGYC 2 cut(s) 162, 320
BsaJI CCNNGG 2 cut(s) 170, 787
BsaWI WCCGGW 2 cut(s) 472, 545
Bsc4I CCNNNNNNNGG 2 cut(s) 187, 552
Bse118I RCCGGY 1 cut(s) 117
Bse1I ACTGG 2 cut(s) 27, 626
Bse21I CCTNAGG 1 cut(s) 489
BseBI CCWGG 3 cut(s) 100, 464, 756
BseCI ATCGAT 1 cut(s) 934
BseDI CCNNGG 2 cut(s) 170, 787
BseGI GGATG 2 cut(s) 751, 934
BseLI CCNNNNNNNGG 2 cut(s) 187, 552
BseMII CTCAG 3 cut(s) 23, 30, 480
BseNI ACTGG 2 cut(s) 27, 626
BseRI GAGGAG 6 cut(s) 275, 278, 304, 353, 356, 359
BseSI GKGCMC 1 cut(s) 64
BseXI GCAGC 2 cut(s) 100, 433
Bsh1236I CGCG 1 cut(s) 208
BshFI GGCC 3 cut(s) 62, 311, 414
BshVI ATCGAT 1 cut(s) 934
BsiSI CCGG 6 cut(s) 118, 172, 399, 415, 473, 546
BslFI GGGAC 2 cut(s) 254, 463
BslI CCNNNNNNNGG 2 cut(s) 187, 552
BsmAI GTCTC 2 cut(s) 28, 813
BsmFI GGGAC 2 cut(s) 254, 463
BsnI GGCC 3 cut(s) 62, 311, 414
Bsp120I GGGCCC 1 cut(s) 60
Bsp1286I GDGCHC 1 cut(s) 64
Bsp143I GATC 3 cut(s) 469, 931, 935
BspACI CCGC 4 cut(s) 121, 191, 208, 317
BspANI GGCC 3 cut(s) 62, 311, 414
BspCNI CTCAG 3 cut(s) 24, 29, 481
BspDI ATCGAT 1 cut(s) 934
BspFNI CGCG 1 cut(s) 208
BspHI TCATGA 1 cut(s) 108
BspLI GGNNCC 9 cut(s) 62, 116, 176, 348, 413, 451, 452, 471, 675
BspMAI CTGCAG 1 cut(s) 682
BspMI ACCTGC 1 cut(s) 211
BspPI GGATC 2 cut(s) 464, 477
BsrFI RCCGGY 1 cut(s) 117
BsrI ACTGG 2 cut(s) 27, 626
BssAI RCCGGY 1 cut(s) 117
BssECI CCNNGG 2 cut(s) 170, 787
BssMI GATC 3 cut(s) 469, 931, 935
BssNI GRCGYC 2 cut(s) 162, 320
BssT1I CCWWGG 1 cut(s) 787
Bst2UI CCWGG 3 cut(s) 100, 464, 756
Bst4CI ACNGT 2 cut(s) 9, 576
Bst6I CTCTTC 6 cut(s) 206, 243, 269, 281, 588, 822
BstACI GRCGYC 2 cut(s) 162, 320
BstBAI YACGTR 1 cut(s) 355
BstDEI CTNAG 3 cut(s) 16, 32, 489
BstF5I GGATG 2 cut(s) 751, 934
BstFNI CGCG 1 cut(s) 208
BstKTI GATC 3 cut(s) 472, 934, 938
BstMAI GTCTC 2 cut(s) 28, 813
BstMBI GATC 3 cut(s) 469, 931, 935
BstMWI GCNNNNNNNGC 2 cut(s) 226, 317
BstNI CCWGG 3 cut(s) 100, 464, 756
BstSCI CCNGG 5 cut(s) 98, 170, 398, 462, 754
BstSFI CTRYAG 2 cut(s) 652, 678
BstSLI GKGCMC 1 cut(s) 64
BstUI CGCG 1 cut(s) 208
BstV1I GCAGC 2 cut(s) 100, 433
BstV2I GAAGAC 2 cut(s) 159, 339
BstX2I RGATCY 1 cut(s) 469
BstYI RGATCY 1 cut(s) 469
Bsu15I ATCGAT 1 cut(s) 934
Bsu36I CCTNAGG 1 cut(s) 489
BsuI GTATCC 2 cut(s) 190, 487
BsuRI GGCC 3 cut(s) 62, 311, 414
BsuTUI ATCGAT 1 cut(s) 934
BtsCI GGATG 2 cut(s) 751, 934
BtsIMutI CAGTG 1 cut(s) 581
BveI ACCTGC 1 cut(s) 211
CciI TCATGA 1 cut(s) 108
Cfr10I RCCGGY 1 cut(s) 117
Cfr13I GGNCC 5 cut(s) 60, 61, 412, 450, 543
ClaI ATCGAT 1 cut(s) 934
CseI GACGC 2 cut(s) 309, 374
Csp6I GTAC 1 cut(s) 530
CviAII CATG 3 cut(s) 109, 559, 842
CviQI GTAC 1 cut(s) 530
DdeI CTNAG 3 cut(s) 16, 32, 489
DpnI GATC 3 cut(s) 471, 933, 937
DpnII GATC 3 cut(s) 469, 931, 935
DraIII CACNNNGTG 1 cut(s) 358
DrdI GACNNNNNNGTC 1 cut(s) 161
DriI GACNNNNNGTC 1 cut(s) 250
DseDI GACNNNNNNGTC 1 cut(s) 161
Eam1104I CTCTTC 6 cut(s) 206, 243, 269, 281, 588, 822
Eam1105I GACNNNNNGTC 1 cut(s) 250
EarI CTCTTC 6 cut(s) 206, 243, 269, 281, 588, 822
Eco130I CCWWGG 1 cut(s) 787
Eco24I GRGCYC 1 cut(s) 64
Eco47I GGWCC 2 cut(s) 450, 543
Eco57I CTGAAG 1 cut(s) 612
Eco72I CACGTG 1 cut(s) 355
Eco81I CCTNAGG 1 cut(s) 489
EcoO109I RGGNCCY 3 cut(s) 60, 61, 450
EcoRII CCWGG 3 cut(s) 98, 462, 754
EcoT14I CCWWGG 1 cut(s) 787
EcoT22I ATGCAT 1 cut(s) 696
EcoT38I GRGCYC 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 787
FaeI CATG 3 cut(s) 112, 562, 845
FaqI GGGAC 2 cut(s) 254, 463
FatI CATG 3 cut(s) 108, 558, 841
FauI CCCGC 1 cut(s) 184
Fnu4HI GCNGC 3 cut(s) 89, 318, 422
FokI GGATG 2 cut(s) 738, 941
FriOI GRGCYC 1 cut(s) 64
Fsp4HI GCNGC 3 cut(s) 89, 318, 422
FspBI CTAG 1 cut(s) 857
GluI GCNGC 3 cut(s) 89, 318, 422
GsuI CTGGAG 1 cut(s) 485
HaeIII GGCC 3 cut(s) 62, 311, 414
HapII CCGG 6 cut(s) 118, 172, 399, 415, 473, 546
HgaI GACGC 2 cut(s) 309, 374
Hin1I GRCGYC 2 cut(s) 162, 320
Hin1II CATG 3 cut(s) 112, 562, 845
HincII GTYRAC 2 cut(s) 630, 810
HindII GTYRAC 2 cut(s) 630, 810
HindIII AAGCTT 2 cut(s) 227, 392
HinfI GANTC 2 cut(s) 251, 683
HpaII CCGG 6 cut(s) 118, 172, 399, 415, 473, 546
HphI GGTGA 2 cut(s) 88, 235
Hpy166II GTNNAC 3 cut(s) 630, 746, 810
Hpy188I TCNGA 4 cut(s) 19, 33, 75, 864
Hpy188III TCNNGA 9 cut(s) 109, 136, 233, 488, 590, 665, 802, 893, 926
Hpy8I GTNNAC 3 cut(s) 630, 746, 810
Hpy99I CGWCG 8 cut(s) 142, 149, 152, 161, 164, 167, 208, 248
HpyAV CCTTC 5 cut(s) 51, 230, 292, 397, 402
HpyCH4III ACNGT 2 cut(s) 9, 576
HpyCH4IV ACGT 2 cut(s) 162, 354
HpyCH4V TGCA 6 cut(s) 128, 680, 694, 709, 769, 908
HpyF10VI GCNNNNNNNGC 2 cut(s) 226, 317
HpyF3I CTNAG 3 cut(s) 16, 32, 489
HpySE526I ACGT 2 cut(s) 162, 354
Hsp92I GRCGYC 2 cut(s) 162, 320
Hsp92II CATG 3 cut(s) 112, 562, 845
KflI GGGWCCC 1 cut(s) 450
Kzo9I GATC 3 cut(s) 469, 931, 935
LmnI GCTCC 3 cut(s) 174, 346, 555
Lsp1109I GCAGC 2 cut(s) 100, 433
LweI GCATC 1 cut(s) 760
MaeI CTAG 1 cut(s) 857
MaeII ACGT 2 cut(s) 162, 354
MaeIII GTNAC 1 cut(s) 133
MalI GATC 3 cut(s) 471, 933, 937
MboI GATC 3 cut(s) 469, 931, 935
MflI RGATCY 1 cut(s) 469
MhlI GDGCHC 1 cut(s) 64
MluCI AATT 1 cut(s) 519
MlyI GAGTC 1 cut(s) 260
Mph1103I ATGCAT 1 cut(s) 696
MseI TTAA 3 cut(s) 390, 722, 873
MslI CAYNNNNRTG 1 cut(s) 666
MspI CCGG 6 cut(s) 118, 172, 399, 415, 473, 546
MspR9I CCNGG 5 cut(s) 100, 172, 400, 464, 756
MvaI CCWGG 3 cut(s) 100, 464, 756
MvnI CGCG 1 cut(s) 208
MwoI GCNNNNNNNGC 2 cut(s) 226, 317
NciI CCSGG 2 cut(s) 172, 400
NdeII GATC 3 cut(s) 469, 931, 935
NlaIII CATG 3 cut(s) 112, 562, 845
NlaIV GGNNCC 9 cut(s) 62, 116, 176, 348, 413, 451, 452, 471, 675
NmuCI GTSAC 1 cut(s) 133
NsiI ATGCAT 1 cut(s) 696
PagI TCATGA 1 cut(s) 108
PaqCI CACCTGC 1 cut(s) 211
PcsI WCGNNNNNNNCGW 2 cut(s) 153, 156
PfeI GAWTC 1 cut(s) 683
PflFI GACNNNGTC 1 cut(s) 161
PfoI TCCNGGA 1 cut(s) 398
PkrI GCNGC 3 cut(s) 90, 319, 423
PleI GAGTC 1 cut(s) 259
PmaCI CACGTG 1 cut(s) 355
PmlI CACGTG 1 cut(s) 355
PpsI GAGTC 1 cut(s) 259
Ppu21I YACGTR 1 cut(s) 355
PpuMI RGGWCCY 1 cut(s) 450
Psp5II RGGWCCY 1 cut(s) 450
Psp6I CCWGG 3 cut(s) 98, 462, 754
PspCI CACGTG 1 cut(s) 355
PspGI CCWGG 3 cut(s) 98, 462, 754
PspN4I GGNNCC 9 cut(s) 62, 116, 176, 348, 413, 451, 452, 471, 675
PspOMI GGGCCC 1 cut(s) 60
PspPI GGNCC 5 cut(s) 60, 61, 412, 450, 543
PspPPI RGGWCCY 1 cut(s) 450
PstI CTGCAG 1 cut(s) 682
PsuI RGATCY 1 cut(s) 469
PsyI GACNNNGTC 1 cut(s) 161
RsaI GTAC 1 cut(s) 531
RsaNI GTAC 1 cut(s) 530
RseI CAYNNNNRTG 1 cut(s) 666
SaqAI TTAA 3 cut(s) 390, 722, 873
SatI GCNGC 3 cut(s) 89, 318, 422
Sau3AI GATC 3 cut(s) 469, 931, 935
Sau96I GGNCC 5 cut(s) 60, 61, 412, 450, 543
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 5 cut(s) 100, 172, 400, 464, 756
SduI GDGCHC 1 cut(s) 64
SfaNI GCATC 1 cut(s) 760
SfcI CTRYAG 2 cut(s) 652, 678
SinI GGWCC 2 cut(s) 450, 543
SmiMI CAYNNNNRTG 1 cut(s) 666
Sse9I AATT 1 cut(s) 519
SsiI CCGC 4 cut(s) 121, 191, 208, 317
SspMI CTAG 1 cut(s) 857
StyD4I CCNGG 5 cut(s) 98, 170, 398, 462, 754
StyI CCWWGG 1 cut(s) 787
TaaI ACNGT 2 cut(s) 9, 576
TaiI ACGT 2 cut(s) 165, 357
TaqI TCGA 2 cut(s) 232, 934
TasI AATT 1 cut(s) 519
TauI GCSGC 1 cut(s) 320
TfiI GAWTC 1 cut(s) 683
Tru1I TTAA 3 cut(s) 390, 722, 873
Tru9I TTAA 3 cut(s) 390, 722, 873
TscAI CASTG 1 cut(s) 581
TseFI GTSAC 1 cut(s) 133
TseI GCWGC 2 cut(s) 88, 421
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 1 cut(s) 751
TspGWI ACGGA 2 cut(s) 676, 708
TspRI CASTG 1 cut(s) 581
Tth111I GACNNNGTC 1 cut(s) 161
VpaK11BI GGWCC 2 cut(s) 450, 543
XspI CTAG 1 cut(s) 857
ZraI GACGTC 1 cut(s) 163
Zsp2I ATGCAT 1 cut(s) 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.