Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0001833

Physical Location & Seq

Genomic Coordinates Reverse (-)

24798955 .. 24801467

Gene Structure 2513 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1108500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1413 bp

ATGTGGAGGAGCACCCGCGTCCCCTACCGCCATGTATCCCCGAGGGAATATTCTCGAATATGCCCTCCGCCGCCCGCGGGCGGCGAGCCGCTCATCCGGGTGTCAAACCACGTGGCTAAATTGGGCCCGCCGAAGGAAGGCCCGAAGCCGAGGCAACTCCTGTCTTTGCCGCCTTTTCCCGGCCACCCTTTGCCGGGGAAGAGGTCACACGAAGACGGCGCCGTTTCGCGCGTCACCGCCGTCAGCTGGGTTAAGTATTACTTCGACGAGATTCGTGACTCTGCCATTCAGTCCCACTTCAACAAAGGCCTTGTTCAGATGGAATGCCCAAATTTGATTGAAAAGGAAGGGCGAGTGAAACCCATGAGACAGATTAGGCCTAGTGATGTAGTGGATGTTGGGGCAAGAGTGTTTGTGCCGGTTTCGGTTGCCGAGAGTAGGGTTTCGAGGAGATTCGACTGCATTCCAAGCGGAACATTAAACCCGAATGCTGATGAGATTAAATACTTGCAGAGGCTTGTCAAGTACAAGGACTCTGCTATCATCGTGCTGAACAAGCCCCCGAAATTGCCGGTCAAGGGTAATCTGCCTGTACATAACGGCATGGATGCATTGGCAGCGGCAGCGTTATCTTATGATTGTAAAGAAGGTCCTAAGTTGGTTCATCGGCTTGACACAGAGAGCAGTGGCCTCCACTTAATGGGGAGAACAAGAGAGAGTGTTTCTCTTCTGCAATGGTTATACACTGACATCAATAAGGGAAAATCGTCATGCAAGGCATGGAACAATGCATGCGATGCAAAATATCAGAGTTATTGGGCATTGGTCATTGGCTCTCCGAAGGAGAAGGAAGGCTTGATCTGTGCTCCTCTTACAAAGGTTGTTCTTGACGATGGGAAGACAGAGAGGGTCATCTTGGCTCATCAATCAGATTTCCAAACTTCCCAACAGGCTATAACTCAATACCGGGTACTTGGTCCTACAATAAATGGATGCTCATGGATTGAGTTACGTCCACTCACTAGTCGGAAGCATCAGCTCCGTGTCCATTGTGCTGAAGCCCTCGGTACGCCAATTGTGGGCGACTTTAAGTACGGGTGGTTTGTGCACAAGAGATGGAAACAAATACCCCAAGTTGATATTGAGCCAACATCTGGGCTACCATACAAGTTGCGGAGGCCAGCAGGCTTGGATGTGCAGAAGGGAAGTGTTCTGTCAAAAGTTCCTCTGTTACATCTACACTGTAGAGAGCTCGTACTTCCGAATATTGCCAAGTTCCTTCTGTTATTGGATCAGAAACTGGACGACCAGCACCCTGCAGTTGTTTCGAAACCAGATCTCCTCCGGTTTTGTGCCTCAATGCCTTCCCACATGAAAATTAGTTGGAATCTCATGTCATCCTACTTAGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001522 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009451 GO:0009982 GO:0009987 GO:0010467 GO:0016070 GO:0016853 GO:0016866 GO:0031119 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PseudoU_synth_2

Protein Analysis

471

Amino Acids

52.74

Weight (kDa)

9.57

Isoelectric Point (pI)

51.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PseudoU_synth_2	PF00849	181 - 352	5.5e-19	RNA pseudouridylate synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001833)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G78910 AT1G78910
fragaria_vesca	FvH4_5g04730 FvH4_5g04730 FvH4_5g04730 FvH4_5g04730
malus_domestica	MD06G1108500.v1.1
prunus_persica	Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1
pyrus_communis	pycom06g10190
rosa_chinensis	RchiOBHm_Chr1g0363621 RchiOBHm_Chr6g0299231 RchiOBHm_Chr7g0194671
rosa_laevigata	RLG00000004184 RLG00000011451
rosa_multiflora	Rmu_sc0000548.1_g000009 Rmu_sc0015513.1_g000002
rosa_roxburghii	Rroxscaffold_3G00261180 Rroxscaffold_3G00261210 Rroxscaffold_7G00168890
rosa_rugosa	Rorug01G0308200 Rorug01G0308200 Rorug06G0286400 Rorug07G0016600 Rorug07G0016700
rosa_samantha	Rh1AG317100 Rh6CG412300 Rh6DG399000 Rh7BG144500 Rh7CG148400 Rh7DG145800
rosa_wichuraiana	Rw1G028120 Rw6G034810 Rw7G012340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	218
AccB7I	CCANNNNNTGG	2 cut(s)	700, 1154
AccBSI	CCGCTC	1 cut(s)	91
AccII	CGCG	4 cut(s)	18, 77, 229, 231
AclWI	GGATC	1 cut(s)	1299
AcoI	YGGCCR	1 cut(s)	181
AcsI	RAATTY	1 cut(s)	331
AcuI	CTGAAG	1 cut(s)	1077
AcvI	CACGTG	1 cut(s)	112
AcyI	GRCGYC	1 cut(s)	219
AfaI	GTAC	6 cut(s)	527, 594, 972, 1069, 1094, 1257
AgsI	TTSAA	2 cut(s)	301, 341
AhlI	ACTAGT	1 cut(s)	1022
AluBI	AGCT	3 cut(s)	246, 1039, 1252
AluI	AGCT	3 cut(s)	246, 1039, 1252
Alw21I	GWGCWC	4 cut(s)	14, 868, 1110, 1254
Alw26I	GTCTC	1 cut(s)	361
Alw44I	GTGCAC	1 cut(s)	1106
AlwI	GGATC	1 cut(s)	1299
AlwNI	CAGNNNCTG	1 cut(s)	1300
Ama87I	CYCGRG	1 cut(s)	40
AoxI	GGCC	7 cut(s)	124, 139, 181, 307, 377, 688, 1178
ApaI	GGGCCC	1 cut(s)	128
ApaLI	GTGCAC	1 cut(s)	1106
ApeKI	GCWGC	2 cut(s)	617, 623
ApoI	RAATTY	1 cut(s)	331
AspLEI	GCGC	2 cut(s)	221, 231
AspS9I	GGNCC	5 cut(s)	124, 125, 140, 650, 977
AsuC2I	CCSGG	4 cut(s)	98, 180, 195, 968
AsuHPI	GGTGA	1 cut(s)	226
AsuII	TTCGAA	1 cut(s)	1328
AvaI	CYCGRG	1 cut(s)	40
AvaII	GGWCC	2 cut(s)	650, 977
BaeGI	GKGCMC	2 cut(s)	128, 1110
BanI	GGYRCC	1 cut(s)	218
BanII	GRGCYC	2 cut(s)	128, 1254
BbrPI	CACGTG	1 cut(s)	112
BbsI	GAAGAC	2 cut(s)	219, 905
Bbv12I	GWGCWC	4 cut(s)	14, 868, 1110, 1254
BbvI	GCAGC	2 cut(s)	629, 635
BccI	CCATC	3 cut(s)	313, 887, 1110
BceAI	ACGGC	4 cut(s)	206, 224, 232, 616
BcgI	CGANNNNNNTGC	2 cut(s)	1308, 1342
BciVI	GTATCC	1 cut(s)	46
BcnI	CCSGG	4 cut(s)	98, 180, 195, 968
BcoDI	GTCTC	1 cut(s)	361
BcuI	ACTAGT	1 cut(s)	1022
BfaI	CTAG	2 cut(s)	381, 1023
BfmI	CTRYAG	2 cut(s)	1243, 1317
BfoI	RGCGCY	1 cut(s)	222
BfuI	GTATCC	1 cut(s)	46
BglII	AGATCT	1 cut(s)	1336
BisI	GCNGC	7 cut(s)	71, 82, 89, 170, 618, 621, 624
BlsI	GCNGC	7 cut(s)	72, 83, 90, 171, 619, 622, 625
Bme1390I	CCNGG	4 cut(s)	98, 180, 195, 968
Bme18I	GGWCC	2 cut(s)	650, 977
BmeT110I	CYCGRG	1 cut(s)	40
BmgT120I	GGNCC	5 cut(s)	124, 125, 140, 650, 977
BmiI	GGNNCC	2 cut(s)	126, 220
BmrFI	CCNGG	4 cut(s)	98, 180, 195, 968
BmsI	GCATC	4 cut(s)	598, 787, 983, 1042
BpiI	GAAGAC	2 cut(s)	219, 905
BplI	GAGNNNNNCTC	2 cut(s)	709, 741
Bpu14I	TTCGAA	1 cut(s)	1328
BpuMI	CCSGG	4 cut(s)	98, 180, 195, 968
BsaAI	YACGTR	1 cut(s)	112
BsaHI	GRCGYC	1 cut(s)	219
BsaJI	CCNNGG	5 cut(s)	41, 75, 149, 194, 1063
BsaWI	WCCGGW	1 cut(s)	1344
BsaXI	ACNNNNNCTCC	2 cut(s)	1323, 1353
Bse118I	RCCGGY	2 cut(s)	418, 571
Bse1I	ACTGG	1 cut(s)	1305
Bse3DI	GCAATG	1 cut(s)	740
BseDI	CCNNGG	5 cut(s)	41, 75, 149, 194, 1063
BseGI	GGATG	6 cut(s)	93, 400, 613, 998, 1198, 1397
BseMI	GCAATG	1 cut(s)	740
BseNI	ACTGG	1 cut(s)	1305
BseRI	GAGGAG	4 cut(s)	22, 463, 858, 1331
BseSI	GKGCMC	2 cut(s)	128, 1110
BseXI	GCAGC	2 cut(s)	629, 635
BseYI	CCCAGC	1 cut(s)	246
BsgI	GTGCAG	1 cut(s)	1217
Bsh1236I	CGCG	4 cut(s)	18, 77, 229, 231
BshFI	GGCC	7 cut(s)	126, 141, 183, 309, 379, 690, 1180
BshNI	GGYRCC	1 cut(s)	218
BsiHKAI	GWGCWC	4 cut(s)	14, 868, 1110, 1254
BsiHKCI	CYCGRG	1 cut(s)	40
BsiSI	CCGG	7 cut(s)	97, 180, 194, 419, 572, 967, 1345
BslFI	GGGAC	2 cut(s)	5, 277
BsmAI	GTCTC	1 cut(s)	361
BsmFI	GGGAC	2 cut(s)	5, 277
BsmI	GAATGC	3 cut(s)	329, 462, 493
BsnI	GGCC	7 cut(s)	126, 141, 183, 309, 379, 690, 1180
BsoBI	CYCGRG	1 cut(s)	40
Bsp119I	TTCGAA	1 cut(s)	1328
Bsp120I	GGGCCC	1 cut(s)	124
Bsp1286I	GDGCHC	5 cut(s)	14, 128, 868, 1110, 1254
Bsp1407I	TGTACA	1 cut(s)	592
Bsp143I	GATC	3 cut(s)	858, 1291, 1336
BspANI	GGCC	7 cut(s)	126, 141, 183, 309, 379, 690, 1180
BspFNI	CGCG	4 cut(s)	18, 77, 229, 231
BspLI	GGNNCC	2 cut(s)	126, 220
BspMAI	CTGCAG	1 cut(s)	1321
BspPI	GGATC	1 cut(s)	1299
BspT104I	TTCGAA	1 cut(s)	1328
BspT107I	GGYRCC	1 cut(s)	218
BsrBI	CCGCTC	1 cut(s)	91
BsrDI	GCAATG	1 cut(s)	740
BsrFI	RCCGGY	2 cut(s)	418, 571
BsrGI	TGTACA	1 cut(s)	592
BsrI	ACTGG	1 cut(s)	1305
BssAI	RCCGGY	2 cut(s)	418, 571
BssECI	CCNNGG	5 cut(s)	41, 75, 149, 194, 1063
BssMI	GATC	3 cut(s)	858, 1291, 1336
BssNI	GRCGYC	1 cut(s)	219
Bst4CI	ACNGT	1 cut(s)	1244
Bst6I	CTCTTC	2 cut(s)	194, 732
BstACI	GRCGYC	1 cut(s)	219
BstAPI	GCANNNNNTGC	1 cut(s)	797
BstAUI	TGTACA	1 cut(s)	592
BstBAI	YACGTR	1 cut(s)	112
BstBI	TTCGAA	1 cut(s)	1328
BstC8I	GCNNGC	7 cut(s)	75, 79, 86, 128, 793, 1182, 1186
BstDEI	CTNAG	2 cut(s)	654, 1405
BstDSI	CCRYGG	1 cut(s)	75
BstF5I	GGATG	6 cut(s)	93, 400, 613, 998, 1198, 1397
BstFNI	CGCG	4 cut(s)	18, 77, 229, 231
BstH2I	RGCGCY	1 cut(s)	222
BstHHI	GCGC	2 cut(s)	221, 231
BstKTI	GATC	3 cut(s)	861, 1294, 1339
BstMAI	GTCTC	1 cut(s)	361
BstMBI	GATC	3 cut(s)	858, 1291, 1336
BstMWI	GCNNNNNNNGC	5 cut(s)	468, 556, 617, 623, 797
BstNSI	RCATGY	1 cut(s)	795
BstSCI	CCNGG	4 cut(s)	96, 178, 193, 966
BstSFI	CTRYAG	2 cut(s)	1243, 1317
BstSLI	GKGCMC	2 cut(s)	128, 1110
BstUI	CGCG	4 cut(s)	18, 77, 229, 231
BstV1I	GCAGC	2 cut(s)	629, 635
BstV2I	GAAGAC	2 cut(s)	219, 905
BstX2I	RGATCY	1 cut(s)	1336
BstYI	RGATCY	1 cut(s)	1336
BsuI	GTATCC	1 cut(s)	46
BsuRI	GGCC	7 cut(s)	126, 141, 183, 309, 379, 690, 1180
BtgI	CCRYGG	1 cut(s)	75
BtgZI	GCGATG	1 cut(s)	810
BtsCI	GGATG	6 cut(s)	93, 400, 613, 998, 1198, 1397
BtsI	GCAGTG	1 cut(s)	691
BtsIMutI	CAGTG	3 cut(s)	691, 744, 1240
Cac8I	GCNNGC	7 cut(s)	75, 79, 86, 128, 793, 1182, 1186
CaiI	CAGNNNCTG	1 cut(s)	1300
CfoI	GCGC	2 cut(s)	221, 231
Cfr10I	RCCGGY	2 cut(s)	418, 571
Cfr13I	GGNCC	5 cut(s)	124, 125, 140, 650, 977
Cfr42I	CCGCGG	1 cut(s)	78
CseI	GACGC	2 cut(s)	7, 220
Csp6I	GTAC	6 cut(s)	526, 593, 971, 1068, 1093, 1256
CspCI	CAANNNNNGTGG	2 cut(s)	93, 128
CviAII	CATG	9 cut(s)	32, 364, 604, 771, 780, 792, 999, 1372, 1393
CviQI	GTAC	6 cut(s)	526, 593, 971, 1068, 1093, 1256
DdeI	CTNAG	2 cut(s)	654, 1405
DinI	GGCGCC	1 cut(s)	220
DpnI	GATC	3 cut(s)	860, 1293, 1338
DpnII	GATC	3 cut(s)	858, 1291, 1336
EaeI	YGGCCR	1 cut(s)	181
Eam1104I	CTCTTC	2 cut(s)	194, 732
EarI	CTCTTC	2 cut(s)	194, 732
EciI	GGCGGA	1 cut(s)	57
Ecl136II	GAGCTC	1 cut(s)	1252
Eco147I	AGGCCT	2 cut(s)	309, 379
Eco24I	GRGCYC	2 cut(s)	128, 1254
Eco47I	GGWCC	2 cut(s)	650, 977
Eco53kI	GAGCTC	1 cut(s)	1252
Eco57I	CTGAAG	1 cut(s)	1077
Eco72I	CACGTG	1 cut(s)	112
Eco88I	CYCGRG	1 cut(s)	40
EcoICRI	GAGCTC	1 cut(s)	1252
EcoO109I	RGGNCCY	1 cut(s)	650
EcoT22I	ATGCAT	2 cut(s)	613, 793
EcoT38I	GRGCYC	2 cut(s)	128, 1254
EgeI	GGCGCC	1 cut(s)	220
EheI	GGCGCC	1 cut(s)	220
FaeI	CATG	9 cut(s)	35, 367, 607, 774, 783, 795, 1002, 1375, 1396
FalI	AAGNNNNNCTT	4 cut(s)	245, 277, 839, 871
FaqI	GGGAC	2 cut(s)	5, 277
FatI	CATG	9 cut(s)	31, 363, 603, 770, 779, 791, 998, 1371, 1392
FauI	CCCGC	4 cut(s)	23, 70, 82, 135
Fnu4HI	GCNGC	7 cut(s)	71, 82, 89, 170, 618, 621, 624
FokI	GGATG	6 cut(s)	80, 407, 620, 1005, 1205, 1384
FriOI	GRGCYC	2 cut(s)	128, 1254
Fsp4HI	GCNGC	7 cut(s)	71, 82, 89, 170, 618, 621, 624
FspBI	CTAG	2 cut(s)	381, 1023
GlaI	GCGC	2 cut(s)	220, 230
GluI	GCNGC	7 cut(s)	71, 82, 89, 170, 618, 621, 624
GsaI	CCCAGC	1 cut(s)	250
HaeII	RGCGCY	1 cut(s)	222
HaeIII	GGCC	7 cut(s)	126, 141, 183, 309, 379, 690, 1180
HapII	CCGG	7 cut(s)	97, 180, 194, 419, 572, 967, 1345
HgaI	GACGC	2 cut(s)	7, 220
HhaI	GCGC	2 cut(s)	221, 231
Hin1I	GRCGYC	1 cut(s)	219
Hin1II	CATG	9 cut(s)	35, 367, 607, 774, 783, 795, 1002, 1375, 1396
Hin6I	GCGC	2 cut(s)	219, 229
HinP1I	GCGC	2 cut(s)	219, 229
HinfI	GANTC	5 cut(s)	271, 278, 453, 533, 1387
HpaII	CCGG	7 cut(s)	97, 180, 194, 419, 572, 967, 1345
HphI	GGTGA	1 cut(s)	226
Hpy166II	GTNNAC	2 cut(s)	1016, 1108
Hpy188I	TCNGA	7 cut(s)	318, 810, 840, 931, 1029, 1263, 1296
Hpy188III	TCNNGA	3 cut(s)	54, 275, 887
Hpy8I	GTNNAC	2 cut(s)	1016, 1108
Hpy99I	CGWCG	1 cut(s)	269
HpyCH4III	ACNGT	1 cut(s)	1244
HpyCH4IV	ACGT	2 cut(s)	111, 1012
HpyF10VI	GCNNNNNNNGC	5 cut(s)	468, 556, 617, 623, 797
HpyF3I	CTNAG	2 cut(s)	654, 1405
HpySE526I	ACGT	2 cut(s)	111, 1012
Hsp92I	GRCGYC	1 cut(s)	219
Hsp92II	CATG	9 cut(s)	35, 367, 607, 774, 783, 795, 1002, 1375, 1396
HspAI	GCGC	2 cut(s)	219, 229
KasI	GGCGCC	1 cut(s)	218
KspI	CCGCGG	1 cut(s)	78
Kzo9I	GATC	3 cut(s)	858, 1291, 1336
LmnI	GCTCC	3 cut(s)	9, 871, 1044
Lsp1109I	GCAGC	2 cut(s)	629, 635
LweI	GCATC	4 cut(s)	598, 787, 983, 1042
MaeI	CTAG	2 cut(s)	381, 1023
MaeII	ACGT	2 cut(s)	111, 1012
MaeIII	GTNAC	5 cut(s)	204, 232, 275, 1008, 1230
MalI	GATC	3 cut(s)	860, 1293, 1338
MbiI	CCGCTC	1 cut(s)	91
MboI	GATC	3 cut(s)	858, 1291, 1336
MboII	GAAGA	4 cut(s)	211, 224, 719, 910
MfeI	CAATTG	1 cut(s)	1074
MflI	RGATCY	1 cut(s)	1336
MhlI	GDGCHC	5 cut(s)	14, 128, 868, 1110, 1254
MluCI	AATT	5 cut(s)	119, 331, 566, 1074, 1377
Mly113I	GGCGCC	1 cut(s)	219
MlyI	GAGTC	2 cut(s)	272, 527
MmeI	TCCRAC	2 cut(s)	1007, 1364
Mph1103I	ATGCAT	2 cut(s)	613, 793
MseI	TTAA	6 cut(s)	252, 479, 501, 698, 1089, 1411
MslI	CAYNNNNRTG	1 cut(s)	98
MspA1I	CMGCKG	3 cut(s)	77, 246, 620
MspI	CCGG	7 cut(s)	97, 180, 194, 419, 572, 967, 1345
MspR9I	CCNGG	4 cut(s)	98, 180, 195, 968
MunI	CAATTG	1 cut(s)	1074
Mva1269I	GAATGC	3 cut(s)	329, 462, 493
MvnI	CGCG	4 cut(s)	18, 77, 229, 231
MwoI	GCNNNNNNNGC	5 cut(s)	468, 556, 617, 623, 797
NarI	GGCGCC	1 cut(s)	219
NciI	CCSGG	4 cut(s)	98, 180, 195, 968
NdeII	GATC	3 cut(s)	858, 1291, 1336
NlaIII	CATG	9 cut(s)	35, 367, 607, 774, 783, 795, 1002, 1375, 1396
NlaIV	GGNNCC	2 cut(s)	126, 220
NmeAIII	GCCGAG	2 cut(s)	174, 457
NmuCI	GTSAC	3 cut(s)	204, 232, 275
NsiI	ATGCAT	2 cut(s)	613, 793
NspI	RCATGY	1 cut(s)	795
NspV	TTCGAA	1 cut(s)	1328
PaeI	GCATGC	1 cut(s)	795
PceI	AGGCCT	2 cut(s)	309, 379
PctI	GAATGC	3 cut(s)	329, 462, 493
PfeI	GAWTC	3 cut(s)	271, 453, 1387
PflMI	CCANNNNNTGG	2 cut(s)	700, 1154
PkrI	GCNGC	7 cut(s)	72, 83, 90, 171, 619, 622, 625
PleI	GAGTC	2 cut(s)	272, 527
PluTI	GGCGCC	1 cut(s)	222
PmaCI	CACGTG	1 cut(s)	112
PmlI	CACGTG	1 cut(s)	112
PpsI	GAGTC	2 cut(s)	272, 527
Ppu21I	YACGTR	1 cut(s)	112
PpuMI	RGGWCCY	1 cut(s)	650
Psp124BI	GAGCTC	1 cut(s)	1254
Psp5II	RGGWCCY	1 cut(s)	650
PspCI	CACGTG	1 cut(s)	112
PspFI	CCCAGC	1 cut(s)	246
PspN4I	GGNNCC	2 cut(s)	126, 220
PspOMI	GGGCCC	1 cut(s)	124
PspPI	GGNCC	5 cut(s)	124, 125, 140, 650, 977
PspPPI	RGGWCCY	1 cut(s)	650
PstI	CTGCAG	1 cut(s)	1321
PstNI	CAGNNNCTG	1 cut(s)	1300
PsuI	RGATCY	1 cut(s)	1336
PvuII	CAGCTG	1 cut(s)	246
RsaI	GTAC	6 cut(s)	527, 594, 972, 1069, 1094, 1257
RsaNI	GTAC	6 cut(s)	526, 593, 971, 1068, 1093, 1256
RseI	CAYNNNNRTG	1 cut(s)	98
SacI	GAGCTC	1 cut(s)	1254
SacII	CCGCGG	1 cut(s)	78
SaqAI	TTAA	6 cut(s)	252, 479, 501, 698, 1089, 1411
SatI	GCNGC	7 cut(s)	71, 82, 89, 170, 618, 621, 624
Sau3AI	GATC	3 cut(s)	858, 1291, 1336
Sau96I	GGNCC	5 cut(s)	124, 125, 140, 650, 977
SchI	GAGTC	2 cut(s)	272, 527
ScrFI	CCNGG	4 cut(s)	98, 180, 195, 968
SduI	GDGCHC	5 cut(s)	14, 128, 868, 1110, 1254
SetI	ASST	8 cut(s)	114, 206, 248, 652, 882, 1015, 1041, 1254
SfaNI	GCATC	4 cut(s)	598, 787, 983, 1042
SfcI	CTRYAG	2 cut(s)	1243, 1317
SfoI	GGCGCC	1 cut(s)	220
Sfr303I	CCGCGG	1 cut(s)	78
SfuI	TTCGAA	1 cut(s)	1328
SgrBI	CCGCGG	1 cut(s)	78
SinI	GGWCC	2 cut(s)	650, 977
SmiMI	CAYNNNNRTG	1 cut(s)	98
SpeI	ACTAGT	1 cut(s)	1022
SphI	GCATGC	1 cut(s)	795
Sse9I	AATT	5 cut(s)	119, 331, 566, 1074, 1377
SseBI	AGGCCT	2 cut(s)	309, 379
SspDI	GGCGCC	1 cut(s)	218
SspI	AATATT	2 cut(s)	50, 1267
SspMI	CTAG	2 cut(s)	381, 1023
SstI	GAGCTC	1 cut(s)	1254
StuI	AGGCCT	2 cut(s)	309, 379
StyD4I	CCNGG	4 cut(s)	96, 178, 193, 966
TaaI	ACNGT	1 cut(s)	1244
TaiI	ACGT	2 cut(s)	114, 1015
TaqI	TCGA	5 cut(s)	55, 264, 446, 456, 1328
TasI	AATT	5 cut(s)	119, 331, 566, 1074, 1377
TatI	WGTACW	2 cut(s)	525, 592
TauI	GCSGC	5 cut(s)	73, 84, 91, 172, 623
TfiI	GAWTC	3 cut(s)	271, 453, 1387
Tru1I	TTAA	6 cut(s)	252, 479, 501, 698, 1089, 1411
Tru9I	TTAA	6 cut(s)	252, 479, 501, 698, 1089, 1411
TscAI	CASTG	3 cut(s)	691, 751, 1247
TseFI	GTSAC	3 cut(s)	204, 232, 275
TseI	GCWGC	2 cut(s)	617, 623
Tsp45I	GTSAC	3 cut(s)	204, 232, 275
TspDTI	ATGAA	2 cut(s)	653, 1388
TspGWI	ACGGA	1 cut(s)	1031
TspRI	CASTG	3 cut(s)	691, 751, 1247
Van91I	CCANNNNNTGG	2 cut(s)	700, 1154
VneI	GTGCAC	1 cut(s)	1106
VpaK11BI	GGWCC	2 cut(s)	650, 977
XapI	RAATTY	1 cut(s)	331
XceI	RCATGY	1 cut(s)	795
XspI	CTAG	2 cut(s)	381, 1023
Zsp2I	ATGCAT	2 cut(s)	613, 793

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.