Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0001833

Physical Location & Seq

Genomic Coordinates Reverse (-)

55522583 .. 55525386

Gene Structure 2804 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00261180.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1377 bp

ATGCGGAGAAGAATCGTAACCTACTGCCACGTGTCACTCTTTACTCGCCAATACTCTACCTTATCTCCTCCACCGCCGGAAACCGCCGGACCGCCGCCGCGCATCCGAGTGTCGAACCACGTCGCCAAACTGGGCCCACCGAAAGAAGGCCCGAAGCCCAGGCAGCTCCTCTCCTTGCCGCCTTTCCCCGGCGGTCGACATTTGCCGGGGAAGAAGAAGTCATGCCAAGACGGCGCCGTTTCACGACCCTCACACGTCACCGCCGTCAGTTGGCTCAAGTATTACTTCCATGAGATTGACGACACCGCCATTCGGTCCCATTTCAACAAAGGCCTTATTAGGCCTAGTGAGGTAATGGAGGAAGGTGCAAGAGTACATGTACCCGTATCGATTGCGGAGAGTAAAGTTTCAAGGAGATTCGACTGCATACCGAGTGAGACATTGTACCCAAATGCGGATGAGATTGACTACTTGCAAAGGCTTGTCAAGTACAAGGACTCTGCTATAATAGTGCTGAACAAGCCCCCAAAATTGCCAGTCAAGGGTAATGTGCCAGTCCATAACAGCATGGATGCATTGGCAGCAGCAGCATTATCTTATGATTATGATGAAGGTCCTAAATTGGTTCATCGGCTTGACAGAGAGAGCAGTGGCCTCCTTTTAATGGGAAGAACAAAAGACAGTGTATCTCTTCTGCATTCGTTATACACTGACATCAACATTGGGAAATCATCATGCAGGGCTTGGAATGATGCATGTAGAACAAAATGTCAGAGGTACTGGGCATTGGTTATAGGGTCGCCCAAGGAAAAGGAAGGCTTAATTTGTGCTCCTCTCATGAAGGTGCTTCTTGACGATGGGAAGACAGAGAGGGTCATCTTGGCTCATCAATCAGGATTAGAAGCTTCCCAACCGGCTCTAACTCAATATCGGGTGCTTGGTCCTACGATAAATGGATGCTCATGGATTGAATTACAACCACTCACTAGCCGAAAGCATCAGCTCCGTGTTCATTGTGCTGAAGCTCTAGGCACGCCAATTGTGGGTGACTATAAGTATGGGTGGTTTGTTCACCGGAGATGGAAACAAATGCCTCAAGTCGATATTGAGCCGGCAACTGGGCTACAATACAAGTTGCGGAGGCCAGCGGGTCTGGATGTACAGAAGGGAAGTGTTCTATCACAAGTCCCTCTTTTACATCTACACTGTAGAGAGCTCGTACTCCCCAATATTGCTAAGTTCCTCGTTTTATTGGATCAGAAGTTAGACAACCGGCACCCTGTAGATAGTTTGAAGCCAGATATCCTTCGGTTTGTGGCATCGATGCCAACCCACATGAAACTTAGTTGGAATCTCATGTCATCTTATTTGGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001522 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009451 GO:0009982 GO:0009987 GO:0010467 GO:0016070 GO:0016853 GO:0016866 GO:0031119 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PseudoU_synth_2

Protein Analysis

458

Amino Acids

51.48

Weight (kDa)

9.54

Isoelectric Point (pI)

54.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PseudoU_synth_2	PF00849	169 - 340	1.6e-21	RNA pseudouridylate synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001833)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G78910 AT1G78910
fragaria_vesca	FvH4_5g04730 FvH4_5g04730 FvH4_5g04730 FvH4_5g04730
malus_domestica	MD06G1108500.v1.1
prunus_persica	Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1
pyrus_communis	pycom06g10190
rosa_chinensis	RchiOBHm_Chr1g0363621 RchiOBHm_Chr6g0299231 RchiOBHm_Chr7g0194671
rosa_laevigata	RLG00000004184 RLG00000011451
rosa_multiflora	Rmu_sc0000548.1_g000009 Rmu_sc0015513.1_g000002
rosa_roxburghii	Rroxscaffold_3G00261180 Rroxscaffold_3G00261210 Rroxscaffold_7G00168890
rosa_rugosa	Rorug01G0308200 Rorug01G0308200 Rorug06G0286400 Rorug07G0016600 Rorug07G0016700
rosa_samantha	Rh1AG317100 Rh6CG412300 Rh6DG399000 Rh7BG144500 Rh7CG148400 Rh7DG145800
rosa_wichuraiana	Rw1G028120 Rw6G034810 Rw7G012340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	233, 1275
AccI	GTMKAC	1 cut(s)	196
AccII	CGCG	1 cut(s)	100
AclWI	GGATC	1 cut(s)	1263
AcuI	CTGAAG	1 cut(s)	1041
AcvI	CACGTG	1 cut(s)	31
AcyI	GRCGYC	1 cut(s)	234
AfaI	GTAC	7 cut(s)	375, 381, 446, 491, 779, 1161, 1221
AfiI	CCNNNNNNNGG	9 cut(s)	146, 188, 270, 312, 454, 542, 664, 1043, 1118
AflIII	ACRYGT	3 cut(s)	30, 253, 376
AgsI	TTSAA	4 cut(s)	325, 411, 971, 1294
AjiI	CACGTC	2 cut(s)	121, 256
AjnI	CCWGG	1 cut(s)	158
AluBI	AGCT	5 cut(s)	166, 905, 1003, 1025, 1216
AluI	AGCT	5 cut(s)	166, 905, 1003, 1025, 1216
Alw21I	GWGCWC	2 cut(s)	832, 1218
Alw26I	GTCTC	1 cut(s)	431
AlwI	GGATC	1 cut(s)	1263
AoxI	GGCC	6 cut(s)	133, 148, 331, 341, 652, 1142
ApaI	GGGCCC	1 cut(s)	137
ApeKI	GCWGC	4 cut(s)	163, 581, 584, 587
AspLEI	GCGC	2 cut(s)	102, 236
AspS9I	GGNCC	7 cut(s)	89, 133, 134, 149, 315, 614, 941
AsuC2I	CCSGG	2 cut(s)	189, 207
AsuHPI	GGTGA	3 cut(s)	250, 1058, 1064
AvaII	GGWCC	4 cut(s)	89, 315, 614, 941
BaeGI	GKGCMC	1 cut(s)	137
BanI	GGYRCC	2 cut(s)	233, 1275
BanII	GRGCYC	2 cut(s)	137, 1218
BbrPI	CACGTG	1 cut(s)	31
BbsI	GAAGAC	1 cut(s)	869
Bbv12I	GWGCWC	2 cut(s)	832, 1218
BbvI	GCAGC	4 cut(s)	175, 593, 596, 599
BccI	CCATC	2 cut(s)	851, 1074
BceAI	ACGGC	3 cut(s)	221, 247, 248
BciT130I	CCWGG	1 cut(s)	160
BcnI	CCSGG	2 cut(s)	189, 207
BcoDI	GTCTC	1 cut(s)	431
BfaI	CTAG	3 cut(s)	345, 987, 1028
BfmI	CTRYAG	2 cut(s)	1207, 1281
BfoI	RGCGCY	1 cut(s)	237
BglI	GCCNNNNNGGC	1 cut(s)	231
BisI	GCNGC	7 cut(s)	95, 98, 164, 179, 582, 585, 588
BlsI	GCNGC	7 cut(s)	96, 99, 165, 180, 583, 586, 589
Bme1390I	CCNGG	3 cut(s)	160, 189, 207
Bme18I	GGWCC	4 cut(s)	89, 315, 614, 941
BmgBI	CACGTC	2 cut(s)	121, 256
BmgT120I	GGNCC	7 cut(s)	89, 133, 134, 149, 315, 614, 941
BmiI	GGNNCC	4 cut(s)	135, 235, 317, 1277
BmrFI	CCNGG	3 cut(s)	160, 189, 207
BmrI	ACTGGG	3 cut(s)	140, 790, 1128
BmsI	GCATC	7 cut(s)	111, 562, 742, 947, 1006, 1314, 1328
BmuI	ACTGGG	3 cut(s)	140, 790, 1128
BpiI	GAAGAC	1 cut(s)	869
BpuEI	CTTGAG	2 cut(s)	260, 1080
BpuMI	CCSGG	2 cut(s)	189, 207
Bsa29I	ATCGAT	2 cut(s)	389, 1322
BsaAI	YACGTR	1 cut(s)	31
BsaHI	GRCGYC	1 cut(s)	234
BsaJI	CCNNGG	4 cut(s)	158, 187, 206, 804
BsaWI	WCCGGW	1 cut(s)	1074
BsaXI	ACNNNNNCTCC	2 cut(s)	49, 79
Bsc4I	CCNNNNNNNGG	9 cut(s)	146, 188, 270, 312, 454, 542, 664, 1043, 1118
Bse118I	RCCGGY	3 cut(s)	913, 1111, 1272
Bse1I	ACTGG	5 cut(s)	135, 536, 554, 785, 1123
BseBI	CCWGG	1 cut(s)	160
BseCI	ATCGAT	2 cut(s)	389, 1322
BseDI	CCNNGG	4 cut(s)	158, 187, 206, 804
BseGI	GGATG	5 cut(s)	102, 463, 577, 962, 1162
BseLI	CCNNNNNNNGG	9 cut(s)	146, 188, 270, 312, 454, 542, 664, 1043, 1118
BseNI	ACTGG	5 cut(s)	135, 536, 554, 785, 1123
BseRI	GAGGAG	3 cut(s)	57, 158, 822
BseSI	GKGCMC	1 cut(s)	137
BseXI	GCAGC	4 cut(s)	175, 593, 596, 599
Bsh1236I	CGCG	1 cut(s)	100
Bsh1285I	CGRYCG	1 cut(s)	196
BshFI	GGCC	6 cut(s)	135, 150, 333, 343, 654, 1144
BshNI	GGYRCC	2 cut(s)	233, 1275
BshVI	ATCGAT	2 cut(s)	389, 1322
BsiEI	CGRYCG	1 cut(s)	196
BsiHKAI	GWGCWC	2 cut(s)	832, 1218
BsiSI	CCGG	8 cut(s)	77, 87, 189, 206, 914, 1075, 1112, 1273
BslFI	GGGAC	2 cut(s)	301, 1172
BslI	CCNNNNNNNGG	9 cut(s)	146, 188, 270, 312, 454, 542, 664, 1043, 1118
BsmAI	GTCTC	1 cut(s)	431
BsmFI	GGGAC	2 cut(s)	301, 1172
BsmI	GAATGC	1 cut(s)	697
BsnI	GGCC	6 cut(s)	135, 150, 333, 343, 654, 1144
Bsp120I	GGGCCC	1 cut(s)	133
Bsp1286I	GDGCHC	3 cut(s)	137, 832, 1218
Bsp1407I	TGTACA	1 cut(s)	1159
Bsp143I	GATC	1 cut(s)	1255
BspANI	GGCC	6 cut(s)	135, 150, 333, 343, 654, 1144
BspDI	ATCGAT	2 cut(s)	389, 1322
BspFNI	CGCG	1 cut(s)	100
BspHI	TCATGA	1 cut(s)	837
BspLI	GGNNCC	4 cut(s)	135, 235, 317, 1277
BspPI	GGATC	1 cut(s)	1263
BspT107I	GGYRCC	2 cut(s)	233, 1275
BsrFI	RCCGGY	3 cut(s)	913, 1111, 1272
BsrGI	TGTACA	1 cut(s)	1159
BsrI	ACTGG	5 cut(s)	135, 536, 554, 785, 1123
BssAI	RCCGGY	3 cut(s)	913, 1111, 1272
BssECI	CCNNGG	4 cut(s)	158, 187, 206, 804
BssMI	GATC	1 cut(s)	1255
BssNI	GRCGYC	1 cut(s)	234
BssT1I	CCWWGG	1 cut(s)	804
Bst2UI	CCWGG	1 cut(s)	160
Bst4CI	ACNGT	2 cut(s)	683, 1208
Bst6I	CTCTTC	1 cut(s)	696
BstACI	GRCGYC	1 cut(s)	234
BstAUI	TGTACA	1 cut(s)	1159
BstBAI	YACGTR	1 cut(s)	31
BstC8I	GCNNGC	3 cut(s)	1034, 1113, 1146
BstDEI	CTNAG	2 cut(s)	1236, 1343
BstF5I	GGATG	5 cut(s)	102, 463, 577, 962, 1162
BstFNI	CGCG	1 cut(s)	100
BstH2I	RGCGCY	1 cut(s)	237
BstHHI	GCGC	2 cut(s)	102, 236
BstKTI	GATC	1 cut(s)	1258
BstMAI	GTCTC	1 cut(s)	431
BstMBI	GATC	1 cut(s)	1255
BstMCI	CGRYCG	1 cut(s)	196
BstMWI	GCNNNNNNNGC	5 cut(s)	163, 231, 520, 581, 587
BstNI	CCWGG	1 cut(s)	160
BstNSI	RCATGY	2 cut(s)	380, 759
BstSCI	CCNGG	3 cut(s)	158, 187, 205
BstSFI	CTRYAG	2 cut(s)	1207, 1281
BstSLI	GKGCMC	1 cut(s)	137
BstUI	CGCG	1 cut(s)	100
BstV1I	GCAGC	4 cut(s)	175, 593, 596, 599
BstV2I	GAAGAC	1 cut(s)	869
Bsu15I	ATCGAT	2 cut(s)	389, 1322
BsuRI	GGCC	6 cut(s)	135, 150, 333, 343, 654, 1144
BsuTUI	ATCGAT	2 cut(s)	389, 1322
BtrI	CACGTC	2 cut(s)	121, 256
BtsCI	GGATG	5 cut(s)	102, 463, 577, 962, 1162
BtsI	GCAGTG	1 cut(s)	655
BtsIMutI	CAGTG	4 cut(s)	655, 688, 708, 1204
Cac8I	GCNNGC	3 cut(s)	1034, 1113, 1146
CciI	TCATGA	1 cut(s)	837
CfoI	GCGC	2 cut(s)	102, 236
Cfr10I	RCCGGY	3 cut(s)	913, 1111, 1272
Cfr13I	GGNCC	7 cut(s)	89, 133, 134, 149, 315, 614, 941
ClaI	ATCGAT	2 cut(s)	389, 1322
CpoI	CGGWCCG	1 cut(s)	89
Csp6I	GTAC	7 cut(s)	374, 380, 445, 490, 778, 1160, 1220
CspI	CGGWCCG	1 cut(s)	89
CviQI	GTAC	7 cut(s)	374, 380, 445, 490, 778, 1160, 1220
DdeI	CTNAG	2 cut(s)	1236, 1343
DinI	GGCGCC	1 cut(s)	235
DpnI	GATC	1 cut(s)	1257
DpnII	GATC	1 cut(s)	1255
Eam1104I	CTCTTC	1 cut(s)	696
EarI	CTCTTC	1 cut(s)	696
Ecl136II	GAGCTC	1 cut(s)	1216
Eco130I	CCWWGG	1 cut(s)	804
Eco147I	AGGCCT	2 cut(s)	333, 343
Eco24I	GRGCYC	2 cut(s)	137, 1218
Eco32I	GATATC	1 cut(s)	1303
Eco47I	GGWCC	4 cut(s)	89, 315, 614, 941
Eco53kI	GAGCTC	1 cut(s)	1216
Eco57I	CTGAAG	1 cut(s)	1041
Eco72I	CACGTG	1 cut(s)	31
EcoICRI	GAGCTC	1 cut(s)	1216
EcoO109I	RGGNCCY	1 cut(s)	614
EcoRII	CCWGG	1 cut(s)	158
EcoRV	GATATC	1 cut(s)	1303
EcoT14I	CCWWGG	1 cut(s)	804
EcoT22I	ATGCAT	2 cut(s)	577, 757
EcoT38I	GRGCYC	2 cut(s)	137, 1218
EgeI	GGCGCC	1 cut(s)	235
EheI	GGCGCC	1 cut(s)	235
ErhI	CCWWGG	1 cut(s)	804
FalI	AAGNNNNNCTT	6 cut(s)	269, 301, 803, 835, 1176, 1208
FaqI	GGGAC	2 cut(s)	301, 1172
FauI	CCCGC	1 cut(s)	1141
FblI	GTMKAC	1 cut(s)	196
Fnu4HI	GCNGC	7 cut(s)	95, 98, 164, 179, 582, 585, 588
FokI	GGATG	5 cut(s)	89, 470, 584, 969, 1169
FriOI	GRGCYC	2 cut(s)	137, 1218
Fsp4HI	GCNGC	7 cut(s)	95, 98, 164, 179, 582, 585, 588
FspBI	CTAG	3 cut(s)	345, 987, 1028
GlaI	GCGC	2 cut(s)	101, 235
GluI	GCNGC	7 cut(s)	95, 98, 164, 179, 582, 585, 588
HaeII	RGCGCY	1 cut(s)	237
HaeIII	GGCC	6 cut(s)	135, 150, 333, 343, 654, 1144
HapII	CCGG	8 cut(s)	77, 87, 189, 206, 914, 1075, 1112, 1273
HhaI	GCGC	2 cut(s)	102, 236
Hin1I	GRCGYC	1 cut(s)	234
Hin6I	GCGC	2 cut(s)	100, 234
HinP1I	GCGC	2 cut(s)	100, 234
HincII	GTYRAC	1 cut(s)	197
HindII	GTYRAC	1 cut(s)	197
HindIII	AAGCTT	1 cut(s)	903
HinfI	GANTC	4 cut(s)	12, 417, 497, 1351
HpaII	CCGG	8 cut(s)	77, 87, 189, 206, 914, 1075, 1112, 1273
HphI	GGTGA	3 cut(s)	250, 1058, 1064
Hpy166II	GTNNAC	2 cut(s)	197, 1072
Hpy188I	TCNGA	3 cut(s)	107, 774, 1260
Hpy188III	TCNNGA	5 cut(s)	243, 838, 851, 894, 1154
Hpy8I	GTNNAC	2 cut(s)	197, 1072
Hpy99I	CGWCG	1 cut(s)	125
HpyAV	CCTTC	7 cut(s)	140, 356, 605, 809, 835, 1159, 1316
HpyCH4III	ACNGT	2 cut(s)	683, 1208
HpyCH4IV	ACGT	3 cut(s)	30, 120, 255
HpyCH4V	TGCA	7 cut(s)	368, 426, 475, 575, 697, 738, 755
HpyF10VI	GCNNNNNNNGC	5 cut(s)	163, 231, 520, 581, 587
HpyF3I	CTNAG	2 cut(s)	1236, 1343
HpySE526I	ACGT	3 cut(s)	30, 120, 255
Hsp92I	GRCGYC	1 cut(s)	234
HspAI	GCGC	2 cut(s)	100, 234
KasI	GGCGCC	1 cut(s)	233
KroI	GCCGGC	1 cut(s)	1111
KroNI	GCCGGC	1 cut(s)	1113
Kzo9I	GATC	1 cut(s)	1255
LmnI	GCTCC	3 cut(s)	171, 835, 1008
Lsp1109I	GCAGC	4 cut(s)	175, 593, 596, 599
LweI	GCATC	7 cut(s)	111, 562, 742, 947, 1006, 1314, 1328
MaeI	CTAG	3 cut(s)	345, 987, 1028
MaeII	ACGT	3 cut(s)	30, 120, 255
MaeIII	GTNAC	4 cut(s)	16, 33, 256, 1046
MalI	GATC	1 cut(s)	1257
MboI	GATC	1 cut(s)	1255
MboII	GAAGA	6 cut(s)	21, 223, 226, 681, 683, 874
MfeI	CAATTG	1 cut(s)	1038
MhlI	GDGCHC	3 cut(s)	137, 832, 1218
MluCI	AATT	5 cut(s)	530, 620, 822, 971, 1038
Mly113I	GGCGCC	1 cut(s)	234
MlyI	GAGTC	1 cut(s)	491
MmeI	TCCRAC	1 cut(s)	1328
Mph1103I	ATGCAT	2 cut(s)	577, 757
MroNI	GCCGGC	1 cut(s)	1111
MseI	TTAA	2 cut(s)	662, 821
MslI	CAYNNNNRTG	2 cut(s)	107, 842
MspA1I	CMGCKG	1 cut(s)	1148
MspI	CCGG	8 cut(s)	77, 87, 189, 206, 914, 1075, 1112, 1273
MspR9I	CCNGG	3 cut(s)	160, 189, 207
MunI	CAATTG	1 cut(s)	1038
Mva1269I	GAATGC	1 cut(s)	697
MvaI	CCWGG	1 cut(s)	160
MvnI	CGCG	1 cut(s)	100
MwoI	GCNNNNNNNGC	5 cut(s)	163, 231, 520, 581, 587
NaeI	GCCGGC	1 cut(s)	1113
NarI	GGCGCC	1 cut(s)	234
NciI	CCSGG	2 cut(s)	189, 207
NdeII	GATC	1 cut(s)	1255
NgoMIV	GCCGGC	1 cut(s)	1111
NlaIV	GGNNCC	4 cut(s)	135, 235, 317, 1277
NmuCI	GTSAC	3 cut(s)	33, 256, 1046
NsiI	ATGCAT	2 cut(s)	577, 757
NspI	RCATGY	2 cut(s)	380, 759
PagI	TCATGA	1 cut(s)	837
PceI	AGGCCT	2 cut(s)	333, 343
PciI	ACATGT	1 cut(s)	376
PcsI	WCGNNNNNNNCGW	1 cut(s)	261
PctI	GAATGC	1 cut(s)	697
PdiI	GCCGGC	1 cut(s)	1113
PfeI	GAWTC	3 cut(s)	12, 417, 1351
PkrI	GCNGC	7 cut(s)	96, 99, 165, 180, 583, 586, 589
PleI	GAGTC	1 cut(s)	491
PluTI	GGCGCC	1 cut(s)	237
PmaCI	CACGTG	1 cut(s)	31
PmlI	CACGTG	1 cut(s)	31
PpsI	GAGTC	1 cut(s)	491
Ppu21I	YACGTR	1 cut(s)	31
PpuMI	RGGWCCY	1 cut(s)	614
PscI	ACATGT	1 cut(s)	376
Psp124BI	GAGCTC	1 cut(s)	1218
Psp5II	RGGWCCY	1 cut(s)	614
Psp6I	CCWGG	1 cut(s)	158
PspCI	CACGTG	1 cut(s)	31
PspGI	CCWGG	1 cut(s)	158
PspN4I	GGNNCC	4 cut(s)	135, 235, 317, 1277
PspOMI	GGGCCC	1 cut(s)	133
PspPI	GGNCC	7 cut(s)	89, 133, 134, 149, 315, 614, 941
PspPPI	RGGWCCY	1 cut(s)	614
RsaI	GTAC	7 cut(s)	375, 381, 446, 491, 779, 1161, 1221
RsaNI	GTAC	7 cut(s)	374, 380, 445, 490, 778, 1160, 1220
RseI	CAYNNNNRTG	2 cut(s)	107, 842
Rsr2I	CGGWCCG	1 cut(s)	89
RsrII	CGGWCCG	1 cut(s)	89
SacI	GAGCTC	1 cut(s)	1218
SalI	GTCGAC	1 cut(s)	195
SaqAI	TTAA	2 cut(s)	662, 821
SatI	GCNGC	7 cut(s)	95, 98, 164, 179, 582, 585, 588
Sau3AI	GATC	1 cut(s)	1255
Sau96I	GGNCC	7 cut(s)	89, 133, 134, 149, 315, 614, 941
SchI	GAGTC	1 cut(s)	491
ScrFI	CCNGG	3 cut(s)	160, 189, 207
SduI	GDGCHC	3 cut(s)	137, 832, 1218
SfaNI	GCATC	7 cut(s)	111, 562, 742, 947, 1006, 1314, 1328
SfcI	CTRYAG	2 cut(s)	1207, 1281
SfoI	GGCGCC	1 cut(s)	235
SinI	GGWCC	4 cut(s)	89, 315, 614, 941
SmiMI	CAYNNNNRTG	2 cut(s)	107, 842
SmlI	CTYRAG	2 cut(s)	275, 1095
SmoI	CTYRAG	2 cut(s)	275, 1095
Sse9I	AATT	5 cut(s)	530, 620, 822, 971, 1038
SseBI	AGGCCT	2 cut(s)	333, 343
SspDI	GGCGCC	1 cut(s)	233
SspI	AATATT	1 cut(s)	1231
SspMI	CTAG	3 cut(s)	345, 987, 1028
SstI	GAGCTC	1 cut(s)	1218
StuI	AGGCCT	2 cut(s)	333, 343
StyD4I	CCNGG	3 cut(s)	158, 187, 205
StyI	CCWWGG	1 cut(s)	804
TaaI	ACNGT	2 cut(s)	683, 1208
TaiI	ACGT	3 cut(s)	33, 123, 258
TaqI	TCGA	6 cut(s)	113, 196, 389, 420, 1101, 1322
TaqII	GACCGA	1 cut(s)	303
TasI	AATT	5 cut(s)	530, 620, 822, 971, 1038
TatI	WGTACW	3 cut(s)	373, 489, 1159
TauI	GCSGC	3 cut(s)	97, 100, 181
TfiI	GAWTC	3 cut(s)	12, 417, 1351
Tru1I	TTAA	2 cut(s)	662, 821
Tru9I	TTAA	2 cut(s)	662, 821
TscAI	CASTG	4 cut(s)	655, 688, 715, 1211
TseFI	GTSAC	3 cut(s)	33, 256, 1046
TseI	GCWGC	4 cut(s)	163, 581, 584, 587
Tsp45I	GTSAC	3 cut(s)	33, 256, 1046
TspDTI	ATGAA	5 cut(s)	617, 624, 854, 1001, 1352
TspGWI	ACGGA	1 cut(s)	995
TspRI	CASTG	4 cut(s)	655, 688, 715, 1211
VpaK11BI	GGWCC	4 cut(s)	89, 315, 614, 941
XceI	RCATGY	2 cut(s)	380, 759
XmiI	GTMKAC	1 cut(s)	196
XspI	CTAG	3 cut(s)	345, 987, 1028
Zsp2I	ATGCAT	2 cut(s)	577, 757

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.