Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0001833

Physical Location & Seq

Genomic Coordinates Reverse (-)

56993054 .. 56995449

Gene Structure 2396 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000004184

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1437 bp

ATGCGGAGAAGAATCGTAACCTACTGCCACATGTCACTCTGTACTCGCCAATACTCTACCTTATCTCCTCCACCGCCGCAGACCGCCGGACCGCCGCCGCTCATCCGAGTGTCGAACCACGTCGCCAAACTGGGCCCACCGAAAGAAGGCCCGAAGCCCAGGCAGCTCCTCTCCTTGCCGCCTTTCCCCGGCGGTCGACATTTGCCGGGGAAGAAGAAGTCATGCCAAGACGGCGCCGTTTCACGACCCTCACACGTCACCGCCGTCAGTTGGCTCAAGTATTACTTCCCTGAGATTGACGACACCGCCATTCGGTCCCATTTCAACAAAGGCCTCGTTCAGATGGAATGCCCGGATTCAATTGGGAAGGAAGGGCAAGTGAAACGTATGAGAAAGATTAGGCCTAGTGAGGTCATGGAGGAAGCTGCAAGAGTATATGTACCCGTATCGATTGCAGAGAGTAAAGTTTCAAGGAGGTTCGACTGCATACCGAGTGAGACATTGTACCCAAATGCGGATGAGATTGACTACTTGCAAAGGCTTGTCAAGTACAAGGACTCTGCTATAATAGTGTTGAACAAGCCCCCAAAATTGCCCGTCAAGGGTAATGTGCCCGTCCATAACAGCATGGATGCATTGGCAGCAGCAGCATTATCTTATGATTATGATGAAGGTCCTAAATTGGTTCATCGGCTTGACAGAGAGAGCAGTGGCCTCCTTTTAATGGGAAGAACAAAAGACAGTGTATCTCTTCTGCATTCGTTATACACTGACGTCAACATTGGGAAATCATCATGCAGGGCTTGGAATGATGCATGTAGGGCAAAATGTCAGAGGTACTGGGCATTGGTTATAGGGTCGCCCAAGGAAAAGGAAGGCTTAATTTGTGCTCCTCTCATGAAGGTGCTTCTTGACGATGGGAAGACAGAGAGGGTCATCTTGGTTCATCAATCAGGATTAGAAGCTTCCCAACCGGCTCTAACTCAATATCGGGTGCTTGGTCCTACGATAAATGGATGCTCATGGATTGAATTACAACCACTCACTAGCCGAAAGCATCAGCTCCGTGTTCATTGTGCTGAAGCTCTAGGCACGCCAATTGTGGGTGACTATAAGTATGGGTGGTTTGTTTACCGGAGATGGAAACAAATGCCTCAAGTCAATATTGAGCCGGCAACTGGACTACAATACAAGTTGCGGAGGCCAGCAGGTCTGGATGTACAGAAGGGAAGTGTTCTATCAAAAGTCCCTCTTTTACATCTACACTGTAGAGAGCTCGTACTCCCCAATATTGCTAAGTTCCTTGTTTTATTGGATCAGAAGTCAGACAACCGGCACCCTGTAGATAGGTTGAAGCCGGATATCCTTCGGTTTGTGGCATCGATGCCGACCCACATGAAACTTAGTTGGAATCTCATGTCATCTTATTTGGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001522 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009451 GO:0009982 GO:0009987 GO:0010467 GO:0016070 GO:0016853 GO:0016866 GO:0031119 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PseudoU_synth_2

Protein Analysis

479

Amino Acids

53.78

Weight (kDa)

9.61

Isoelectric Point (pI)

57.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PseudoU_synth_2	PF00849	189 - 360	2.1e-21	RNA pseudouridylate synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001833)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G78910 AT1G78910
fragaria_vesca	FvH4_5g04730 FvH4_5g04730 FvH4_5g04730 FvH4_5g04730
malus_domestica	MD06G1108500.v1.1
prunus_persica	Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1 Prupe.5G121100_v2.0.a1
pyrus_communis	pycom06g10190
rosa_chinensis	RchiOBHm_Chr1g0363621 RchiOBHm_Chr6g0299231 RchiOBHm_Chr7g0194671
rosa_laevigata	RLG00000004184 RLG00000011451
rosa_multiflora	Rmu_sc0000548.1_g000009 Rmu_sc0015513.1_g000002
rosa_roxburghii	Rroxscaffold_3G00261180 Rroxscaffold_3G00261210 Rroxscaffold_7G00168890
rosa_rugosa	Rorug01G0308200 Rorug01G0308200 Rorug06G0286400 Rorug07G0016600 Rorug07G0016700
rosa_samantha	Rh1AG317100 Rh6CG412300 Rh6DG399000 Rh7BG144500 Rh7CG148400 Rh7DG145800
rosa_wichuraiana	Rw1G028120 Rw6G034810 Rw7G012340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	777
Acc36I	ACCTGC	1 cut(s)	1199
AccB1I	GGYRCC	2 cut(s)	233, 1335
AccBSI	CCGCTC	1 cut(s)	100
AccI	GTMKAC	1 cut(s)	196
AclWI	GGATC	1 cut(s)	1323
AcuI	CTGAAG	1 cut(s)	1101
AcyI	GRCGYC	2 cut(s)	234, 774
AfaI	GTAC	7 cut(s)	43, 441, 506, 551, 839, 1221, 1281
AfiI	CCNNNNNNNGG	9 cut(s)	146, 188, 270, 312, 514, 602, 724, 1103, 1178
AflIII	ACRYGT	2 cut(s)	30, 253
AgsI	TTSAA	6 cut(s)	325, 360, 471, 577, 1031, 1354
AjiI	CACGTC	2 cut(s)	121, 256
AjnI	CCWGG	1 cut(s)	158
AluBI	AGCT	6 cut(s)	166, 425, 965, 1063, 1085, 1276
AluI	AGCT	6 cut(s)	166, 425, 965, 1063, 1085, 1276
Alw21I	GWGCWC	2 cut(s)	892, 1278
Alw26I	GTCTC	1 cut(s)	491
AlwI	GGATC	1 cut(s)	1323
AoxI	GGCC	6 cut(s)	133, 148, 331, 401, 712, 1202
ApaI	GGGCCC	1 cut(s)	137
ApeKI	GCWGC	5 cut(s)	163, 425, 641, 644, 647
AspLEI	GCGC	1 cut(s)	236
AspS9I	GGNCC	7 cut(s)	89, 133, 134, 149, 315, 674, 1001
AsuC2I	CCSGG	3 cut(s)	189, 207, 353
AsuHPI	GGTGA	2 cut(s)	250, 1118
AvaII	GGWCC	4 cut(s)	89, 315, 674, 1001
BaeGI	GKGCMC	2 cut(s)	137, 615
BanI	GGYRCC	2 cut(s)	233, 1335
BanII	GRGCYC	2 cut(s)	137, 1278
BbsI	GAAGAC	1 cut(s)	929
Bbv12I	GWGCWC	2 cut(s)	892, 1278
BbvI	GCAGC	5 cut(s)	175, 412, 653, 656, 659
BccI	CCATC	3 cut(s)	337, 911, 1134
BceAI	ACGGC	3 cut(s)	221, 247, 248
BciT130I	CCWGG	1 cut(s)	160
BcnI	CCSGG	3 cut(s)	189, 207, 353
BcoDI	GTCTC	1 cut(s)	491
BfaI	CTAG	3 cut(s)	405, 1047, 1088
BfmI	CTRYAG	2 cut(s)	1267, 1341
BfoI	RGCGCY	1 cut(s)	237
BfuAI	ACCTGC	1 cut(s)	1199
BglI	GCCNNNNNGGC	1 cut(s)	231
BisI	GCNGC	9 cut(s)	77, 95, 98, 164, 179, 426, 642, 645, 648
BlsI	GCNGC	9 cut(s)	78, 96, 99, 165, 180, 427, 643, 646, 649
Bme1390I	CCNGG	4 cut(s)	160, 189, 207, 353
Bme18I	GGWCC	4 cut(s)	89, 315, 674, 1001
BmgBI	CACGTC	2 cut(s)	121, 256
BmgT120I	GGNCC	7 cut(s)	89, 133, 134, 149, 315, 674, 1001
BmiI	GGNNCC	4 cut(s)	135, 235, 317, 1337
BmrFI	CCNGG	4 cut(s)	160, 189, 207, 353
BmrI	ACTGGG	2 cut(s)	140, 850
BmsI	GCATC	6 cut(s)	622, 802, 1007, 1066, 1374, 1388
BmuI	ACTGGG	2 cut(s)	140, 850
BpiI	GAAGAC	1 cut(s)	929
BpuEI	CTTGAG	2 cut(s)	260, 1140
BpuMI	CCSGG	3 cut(s)	189, 207, 353
Bsa29I	ATCGAT	2 cut(s)	449, 1382
BsaHI	GRCGYC	2 cut(s)	234, 774
BsaJI	CCNNGG	4 cut(s)	158, 187, 206, 864
BsaWI	WCCGGW	1 cut(s)	1134
BsaXI	ACNNNNNCTCC	2 cut(s)	49, 79
Bsc4I	CCNNNNNNNGG	9 cut(s)	146, 188, 270, 312, 514, 602, 724, 1103, 1178
Bse118I	RCCGGY	3 cut(s)	973, 1171, 1332
Bse1I	ACTGG	3 cut(s)	135, 845, 1183
BseBI	CCWGG	1 cut(s)	160
BseCI	ATCGAT	2 cut(s)	449, 1382
BseDI	CCNNGG	4 cut(s)	158, 187, 206, 864
BseGI	GGATG	5 cut(s)	102, 523, 637, 1022, 1222
BseLI	CCNNNNNNNGG	9 cut(s)	146, 188, 270, 312, 514, 602, 724, 1103, 1178
BseMII	CTCAG	1 cut(s)	282
BseNI	ACTGG	3 cut(s)	135, 845, 1183
BseRI	GAGGAG	3 cut(s)	57, 158, 882
BseSI	GKGCMC	2 cut(s)	137, 615
BseXI	GCAGC	5 cut(s)	175, 412, 653, 656, 659
Bsh1285I	CGRYCG	1 cut(s)	196
BshFI	GGCC	6 cut(s)	135, 150, 333, 403, 714, 1204
BshNI	GGYRCC	2 cut(s)	233, 1335
BshVI	ATCGAT	2 cut(s)	449, 1382
BsiEI	CGRYCG	1 cut(s)	196
BsiHKAI	GWGCWC	2 cut(s)	892, 1278
BsiSI	CCGG	9 cut(s)	87, 189, 206, 353, 974, 1135, 1172, 1333, 1358
BslFI	GGGAC	2 cut(s)	301, 1232
BslI	CCNNNNNNNGG	9 cut(s)	146, 188, 270, 312, 514, 602, 724, 1103, 1178
BsmAI	GTCTC	1 cut(s)	491
BsmFI	GGGAC	2 cut(s)	301, 1232
BsmI	GAATGC	2 cut(s)	353, 757
BsnI	GGCC	6 cut(s)	135, 150, 333, 403, 714, 1204
Bsp120I	GGGCCC	1 cut(s)	133
Bsp1286I	GDGCHC	4 cut(s)	137, 615, 892, 1278
Bsp1407I	TGTACA	1 cut(s)	1219
Bsp143I	GATC	1 cut(s)	1315
BspANI	GGCC	6 cut(s)	135, 150, 333, 403, 714, 1204
BspCNI	CTCAG	1 cut(s)	283
BspDI	ATCGAT	2 cut(s)	449, 1382
BspHI	TCATGA	1 cut(s)	897
BspLI	GGNNCC	4 cut(s)	135, 235, 317, 1337
BspMI	ACCTGC	1 cut(s)	1199
BspPI	GGATC	1 cut(s)	1323
BspT107I	GGYRCC	2 cut(s)	233, 1335
BsrBI	CCGCTC	1 cut(s)	100
BsrFI	RCCGGY	3 cut(s)	973, 1171, 1332
BsrGI	TGTACA	1 cut(s)	1219
BsrI	ACTGG	3 cut(s)	135, 845, 1183
BssAI	RCCGGY	3 cut(s)	973, 1171, 1332
BssECI	CCNNGG	4 cut(s)	158, 187, 206, 864
BssMI	GATC	1 cut(s)	1315
BssNI	GRCGYC	2 cut(s)	234, 774
BssT1I	CCWWGG	1 cut(s)	864
Bst2UI	CCWGG	1 cut(s)	160
Bst4CI	ACNGT	2 cut(s)	743, 1268
Bst6I	CTCTTC	1 cut(s)	756
BstACI	GRCGYC	2 cut(s)	234, 774
BstAUI	TGTACA	1 cut(s)	1219
BstC8I	GCNNGC	3 cut(s)	1094, 1173, 1206
BstDEI	CTNAG	3 cut(s)	291, 1296, 1403
BstF5I	GGATG	5 cut(s)	102, 523, 637, 1022, 1222
BstH2I	RGCGCY	1 cut(s)	237
BstHHI	GCGC	1 cut(s)	236
BstKTI	GATC	1 cut(s)	1318
BstMAI	GTCTC	1 cut(s)	491
BstMBI	GATC	1 cut(s)	1315
BstMCI	CGRYCG	1 cut(s)	196
BstMWI	GCNNNNNNNGC	5 cut(s)	163, 231, 641, 647, 821
BstNI	CCWGG	1 cut(s)	160
BstNSI	RCATGY	2 cut(s)	34, 819
BstSCI	CCNGG	4 cut(s)	158, 187, 205, 351
BstSFI	CTRYAG	2 cut(s)	1267, 1341
BstSLI	GKGCMC	2 cut(s)	137, 615
BstV1I	GCAGC	5 cut(s)	175, 412, 653, 656, 659
BstV2I	GAAGAC	1 cut(s)	929
Bsu15I	ATCGAT	2 cut(s)	449, 1382
BsuRI	GGCC	6 cut(s)	135, 150, 333, 403, 714, 1204
BsuTUI	ATCGAT	2 cut(s)	449, 1382
BtrI	CACGTC	2 cut(s)	121, 256
BtsCI	GGATG	5 cut(s)	102, 523, 637, 1022, 1222
BtsI	GCAGTG	1 cut(s)	715
BtsIMutI	CAGTG	4 cut(s)	715, 748, 768, 1264
BveI	ACCTGC	1 cut(s)	1199
Cac8I	GCNNGC	3 cut(s)	1094, 1173, 1206
CciI	TCATGA	1 cut(s)	897
CfoI	GCGC	1 cut(s)	236
Cfr10I	RCCGGY	3 cut(s)	973, 1171, 1332
Cfr13I	GGNCC	7 cut(s)	89, 133, 134, 149, 315, 674, 1001
ClaI	ATCGAT	2 cut(s)	449, 1382
CpoI	CGGWCCG	1 cut(s)	89
Csp6I	GTAC	7 cut(s)	42, 440, 505, 550, 838, 1220, 1280
CspI	CGGWCCG	1 cut(s)	89
CviQI	GTAC	7 cut(s)	42, 440, 505, 550, 838, 1220, 1280
DdeI	CTNAG	3 cut(s)	291, 1296, 1403
DinI	GGCGCC	1 cut(s)	235
DpnI	GATC	1 cut(s)	1317
DpnII	GATC	1 cut(s)	1315
Eam1104I	CTCTTC	1 cut(s)	756
EarI	CTCTTC	1 cut(s)	756
Ecl136II	GAGCTC	1 cut(s)	1276
Eco130I	CCWWGG	1 cut(s)	864
Eco147I	AGGCCT	2 cut(s)	333, 403
Eco24I	GRGCYC	2 cut(s)	137, 1278
Eco32I	GATATC	1 cut(s)	1363
Eco47I	GGWCC	4 cut(s)	89, 315, 674, 1001
Eco53kI	GAGCTC	1 cut(s)	1276
Eco57I	CTGAAG	1 cut(s)	1101
EcoICRI	GAGCTC	1 cut(s)	1276
EcoO109I	RGGNCCY	1 cut(s)	674
EcoRII	CCWGG	1 cut(s)	158
EcoRV	GATATC	1 cut(s)	1363
EcoT14I	CCWWGG	1 cut(s)	864
EcoT22I	ATGCAT	2 cut(s)	637, 817
EcoT38I	GRGCYC	2 cut(s)	137, 1278
EgeI	GGCGCC	1 cut(s)	235
EheI	GGCGCC	1 cut(s)	235
ErhI	CCWWGG	1 cut(s)	864
FalI	AAGNNNNNCTT	6 cut(s)	269, 301, 863, 895, 1236, 1268
FaqI	GGGAC	2 cut(s)	301, 1232
FblI	GTMKAC	1 cut(s)	196
Fnu4HI	GCNGC	9 cut(s)	77, 95, 98, 164, 179, 426, 642, 645, 648
FokI	GGATG	5 cut(s)	89, 530, 644, 1029, 1229
FriOI	GRGCYC	2 cut(s)	137, 1278
Fsp4HI	GCNGC	9 cut(s)	77, 95, 98, 164, 179, 426, 642, 645, 648
FspBI	CTAG	3 cut(s)	405, 1047, 1088
GlaI	GCGC	1 cut(s)	235
GluI	GCNGC	9 cut(s)	77, 95, 98, 164, 179, 426, 642, 645, 648
HaeII	RGCGCY	1 cut(s)	237
HaeIII	GGCC	6 cut(s)	135, 150, 333, 403, 714, 1204
HapII	CCGG	9 cut(s)	87, 189, 206, 353, 974, 1135, 1172, 1333, 1358
HhaI	GCGC	1 cut(s)	236
Hin1I	GRCGYC	2 cut(s)	234, 774
Hin6I	GCGC	1 cut(s)	234
HinP1I	GCGC	1 cut(s)	234
HincII	GTYRAC	2 cut(s)	197, 778
HindII	GTYRAC	2 cut(s)	197, 778
HindIII	AAGCTT	1 cut(s)	963
HinfI	GANTC	4 cut(s)	12, 356, 557, 1411
HpaII	CCGG	9 cut(s)	87, 189, 206, 353, 974, 1135, 1172, 1333, 1358
HphI	GGTGA	2 cut(s)	250, 1118
Hpy166II	GTNNAC	3 cut(s)	197, 778, 1132
Hpy188I	TCNGA	5 cut(s)	107, 342, 834, 1320, 1327
Hpy188III	TCNNGA	5 cut(s)	243, 898, 911, 954, 1214
Hpy8I	GTNNAC	3 cut(s)	197, 778, 1132
Hpy99I	CGWCG	1 cut(s)	125
HpyAV	CCTTC	8 cut(s)	140, 361, 365, 665, 869, 895, 1219, 1376
HpyCH4III	ACNGT	2 cut(s)	743, 1268
HpyCH4IV	ACGT	4 cut(s)	120, 255, 385, 774
HpyCH4V	TGCA	8 cut(s)	428, 455, 486, 535, 635, 757, 798, 815
HpyF10VI	GCNNNNNNNGC	5 cut(s)	163, 231, 641, 647, 821
HpyF3I	CTNAG	3 cut(s)	291, 1296, 1403
HpySE526I	ACGT	4 cut(s)	120, 255, 385, 774
Hsp92I	GRCGYC	2 cut(s)	234, 774
HspAI	GCGC	1 cut(s)	234
KasI	GGCGCC	1 cut(s)	233
KroI	GCCGGC	1 cut(s)	1171
KroNI	GCCGGC	1 cut(s)	1173
Kzo9I	GATC	1 cut(s)	1315
LmnI	GCTCC	3 cut(s)	171, 895, 1068
Lsp1109I	GCAGC	5 cut(s)	175, 412, 653, 656, 659
LweI	GCATC	6 cut(s)	622, 802, 1007, 1066, 1374, 1388
MaeI	CTAG	3 cut(s)	405, 1047, 1088
MaeII	ACGT	4 cut(s)	120, 255, 385, 774
MaeIII	GTNAC	4 cut(s)	16, 33, 256, 1106
MalI	GATC	1 cut(s)	1317
MbiI	CCGCTC	1 cut(s)	100
MboI	GATC	1 cut(s)	1315
MboII	GAAGA	6 cut(s)	21, 223, 226, 741, 743, 934
MfeI	CAATTG	2 cut(s)	360, 1098
MhlI	GDGCHC	4 cut(s)	137, 615, 892, 1278
MluCI	AATT	6 cut(s)	360, 590, 680, 882, 1031, 1098
Mly113I	GGCGCC	1 cut(s)	234
MlyI	GAGTC	1 cut(s)	551
MmeI	TCCRAC	1 cut(s)	1388
Mph1103I	ATGCAT	2 cut(s)	637, 817
MroNI	GCCGGC	1 cut(s)	1171
MseI	TTAA	2 cut(s)	722, 881
MslI	CAYNNNNRTG	2 cut(s)	107, 902
MspI	CCGG	9 cut(s)	87, 189, 206, 353, 974, 1135, 1172, 1333, 1358
MspR9I	CCNGG	4 cut(s)	160, 189, 207, 353
MunI	CAATTG	2 cut(s)	360, 1098
Mva1269I	GAATGC	2 cut(s)	353, 757
MvaI	CCWGG	1 cut(s)	160
MwoI	GCNNNNNNNGC	5 cut(s)	163, 231, 641, 647, 821
NaeI	GCCGGC	1 cut(s)	1173
NarI	GGCGCC	1 cut(s)	234
NciI	CCSGG	3 cut(s)	189, 207, 353
NdeII	GATC	1 cut(s)	1315
NgoMIV	GCCGGC	1 cut(s)	1171
NlaIV	GGNNCC	4 cut(s)	135, 235, 317, 1337
NmuCI	GTSAC	3 cut(s)	33, 256, 1106
NsiI	ATGCAT	2 cut(s)	637, 817
NspI	RCATGY	2 cut(s)	34, 819
PagI	TCATGA	1 cut(s)	897
PceI	AGGCCT	2 cut(s)	333, 403
PciI	ACATGT	1 cut(s)	30
PcsI	WCGNNNNNNNCGW	1 cut(s)	261
PctI	GAATGC	2 cut(s)	353, 757
PdiI	GCCGGC	1 cut(s)	1173
PfeI	GAWTC	3 cut(s)	12, 356, 1411
PkrI	GCNGC	9 cut(s)	78, 96, 99, 165, 180, 427, 643, 646, 649
PleI	GAGTC	1 cut(s)	551
PluTI	GGCGCC	1 cut(s)	237
PpsI	GAGTC	1 cut(s)	551
PpuMI	RGGWCCY	1 cut(s)	674
PscI	ACATGT	1 cut(s)	30
Psp124BI	GAGCTC	1 cut(s)	1278
Psp5II	RGGWCCY	1 cut(s)	674
Psp6I	CCWGG	1 cut(s)	158
PspGI	CCWGG	1 cut(s)	158
PspN4I	GGNNCC	4 cut(s)	135, 235, 317, 1337
PspOMI	GGGCCC	1 cut(s)	133
PspPI	GGNCC	7 cut(s)	89, 133, 134, 149, 315, 674, 1001
PspPPI	RGGWCCY	1 cut(s)	674
RsaI	GTAC	7 cut(s)	43, 441, 506, 551, 839, 1221, 1281
RsaNI	GTAC	7 cut(s)	42, 440, 505, 550, 838, 1220, 1280
RseI	CAYNNNNRTG	2 cut(s)	107, 902
Rsr2I	CGGWCCG	1 cut(s)	89
RsrII	CGGWCCG	1 cut(s)	89
SacI	GAGCTC	1 cut(s)	1278
SalI	GTCGAC	1 cut(s)	195
SaqAI	TTAA	2 cut(s)	722, 881
SatI	GCNGC	9 cut(s)	77, 95, 98, 164, 179, 426, 642, 645, 648
Sau3AI	GATC	1 cut(s)	1315
Sau96I	GGNCC	7 cut(s)	89, 133, 134, 149, 315, 674, 1001
SchI	GAGTC	1 cut(s)	551
ScrFI	CCNGG	4 cut(s)	160, 189, 207, 353
SduI	GDGCHC	4 cut(s)	137, 615, 892, 1278
SfaNI	GCATC	6 cut(s)	622, 802, 1007, 1066, 1374, 1388
SfcI	CTRYAG	2 cut(s)	1267, 1341
SfoI	GGCGCC	1 cut(s)	235
SinI	GGWCC	4 cut(s)	89, 315, 674, 1001
SmiMI	CAYNNNNRTG	2 cut(s)	107, 902
SmlI	CTYRAG	2 cut(s)	275, 1155
SmoI	CTYRAG	2 cut(s)	275, 1155
Sse9I	AATT	6 cut(s)	360, 590, 680, 882, 1031, 1098
SseBI	AGGCCT	2 cut(s)	333, 403
SspDI	GGCGCC	1 cut(s)	233
SspI	AATATT	2 cut(s)	1165, 1291
SspMI	CTAG	3 cut(s)	405, 1047, 1088
SstI	GAGCTC	1 cut(s)	1278
StuI	AGGCCT	2 cut(s)	333, 403
StyD4I	CCNGG	4 cut(s)	158, 187, 205, 351
StyI	CCWWGG	1 cut(s)	864
TaaI	ACNGT	2 cut(s)	743, 1268
TaiI	ACGT	4 cut(s)	123, 258, 388, 777
TaqI	TCGA	5 cut(s)	113, 196, 449, 480, 1382
TaqII	GACCGA	1 cut(s)	303
TasI	AATT	6 cut(s)	360, 590, 680, 882, 1031, 1098
TatI	WGTACW	3 cut(s)	41, 549, 1219
TauI	GCSGC	4 cut(s)	79, 97, 100, 181
TfiI	GAWTC	3 cut(s)	12, 356, 1411
Tru1I	TTAA	2 cut(s)	722, 881
Tru9I	TTAA	2 cut(s)	722, 881
TscAI	CASTG	4 cut(s)	715, 748, 775, 1271
TseFI	GTSAC	3 cut(s)	33, 256, 1106
TseI	GCWGC	5 cut(s)	163, 425, 641, 644, 647
Tsp45I	GTSAC	3 cut(s)	33, 256, 1106
TspDTI	ATGAA	6 cut(s)	677, 684, 914, 935, 1061, 1412
TspGWI	ACGGA	1 cut(s)	1055
TspRI	CASTG	4 cut(s)	715, 748, 775, 1271
VpaK11BI	GGWCC	4 cut(s)	89, 315, 674, 1001
XceI	RCATGY	2 cut(s)	34, 819
XmiI	GTMKAC	1 cut(s)	196
XspI	CTAG	3 cut(s)	405, 1047, 1088
ZraI	GACGTC	1 cut(s)	775
Zsp2I	ATGCAT	2 cut(s)	637, 817

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.