Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0006613

Physical Location & Seq

Genomic Coordinates Forward (+)

27069602 .. 27075662

Gene Structure 6061 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1128300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3264 bp

ATGGCAGATTTGTCATCCGATTACGGAGGAGAAGTGAAACGGTGCCCTATTCACCGCAAGCGACCGAGACCGCCGAGAAGCGAAGCGAGTACGGCGGTTTCGGTCCACGTGAAGCAGCTCGACGTCCCTCTCACGTGTCCGGAAACCGGTTCTCCGGTCGATTCAATTTGTCTCCAACCGGATCGCCCTTACACGATCGGGCGGAGCAATCGTGGTTGTCATTTCGTCTTCGCCGATCGCCGTGTGGGTAAGAAACATTGCCAGATTATTTATAATTCTTTTGATCGGAAACTGTATGTTATTGACGGCGCATTGATTTCGGTTCCTCAATTTAGGAATCGATTGGAATTGGAAGAGTGTTGTAATGTTAGGGTTTCTTTGAATGGGGTTTTTGTGAATGGGATTAGGGTTAGGGAAGGCGTGGCGGTGGAGTTGTCGGATGGAGACCGGGTCTCACTCGGTTGCTCAACTGAGAATTGTTGTAATCCGATTCGGATTGGATTTGTTGTTAATAAGATTGTTTGTGAGGACCAAATTGTACAGAGTTTTGATAACCCCCAAGGACCCGTTAAACCTTTATGTGTATCATGTCCATGGGGACGTAAGAGGGTTTTTGCTTCCCGGGCTGAGGGTTCTCCGTCTTCAATCGTTGGAAGAGCAAACTTTCTCTTAAGTGAGTGCAGGCACATTTTGCTTAGCAATGACCCTATATCGTATATTCGAAAATGTTGTAGATATTCTTTTGAGAATGGATTTATACGAAGTAATGATATAGGAGAATCTCCAGGTCTCGATACTGATAGGCTTCCGCTTAACGGGGAAAAATCATTGTTGTGTCAAACCTCACACATAGATTGCGATGTGAAAAATAAAATGCAGTTCACTTCAGTCATGCATCAGTGTGAAAATGTAGTGGCTTCAGAACCAAATGGTGTCATCTTACATAAACAAGACAATGCTCAAGTTCCCTCTGAGACTGCTGTTGCTGATGGGAATCTTGAGACTTCATCTTTCAATTTTACGGGTAGGGAAGACCCTCCTAATTGTGATGGTATTGTCAAGAACAAAACCCGGTGCAGAAATGTTGCTTCACAACCTGGCAAGAACTTTTACTTGAATCGCCTTTCTTTTATGGGCCACAACTCTTCAACTTCAAGTCAAGATTCCGTTATTTCCTTGCCCGAGCTTCTTTATCCTGTGCAAAGTATTTCACAGATGTTTATTGCAACATTTACAAGTGACATTTTGTGGTTTTTGTCATCCTGTGAGATTCCTAGCCATCTGCCAGTTACAGTTGCTTGTCATAATACTGAAAGATGTTGGAGTTCAAGCCCTAATAAAAGAACATCTTCACCTTATCCAGATTTTCCGAAGTTAGTTTTAGTGTATCCCCCGTTTCCCGAGGCCATAGCTTTTGGTAATGACCGCAAGAGACATGGAATTGCTTGCCATCATCCAAAATTGTTGGTCCTGAAAAGAGATGATAGTATTCGTGTCATCATTACCTCTGCAAATTTAGTGTCAACACAGTGGAATGCAGTGACCAATACTGTCTGGTGGCAAGATTTCCCTTGTAGAAGCGCACCTGATTATACTTCCCTTTTTACTCAATTCCACGATGGGGAAACGAATCAAGACTCAAAAACAGATTTTGCTTCTCAGCTGGCTGGATTTATGGCAATGCTACTGACTGATGTACCTAGTCAGGCCCAGTGGATTGTTGACTTGACAAAATATGATTTTGGAGGTGCAACTGGACATTTAGTTGCTTCTGTTCCTGGAGTTCATTCTTATAAAACTCCCTTTATGTTGGATTCCAGGCACTTCGGGCTTGCTGATCATGGTGCGTCATCTGGTGCAAAATTCCTTGGATCTGTTGAAGCATCTGTAGTTGGTCTAAGCTACCTCTTTCGCAATGCAAAAGACTCAAATGGTGCAAAGCTTAAGAAATTGGCTTCATTTCTGAGGAAATCATGTGAACATGCAAAAGCTTTATCAATTGTTTTAACAAGAAACACAAATGTTCCTGCAGATGCAAATGCAGTGAATATTGTTGTTCCTGACTCCAATGAGGGAGACTCTGTTCAACTTGGCTTCTTGCCTAGAAATGTTGCAAAATGGGTCTCTCCACTGTGGGATATTGGACTCTTCAGCTTCTCTGGTTATATCTGTCCTAAAGAAGCTCTTACAGCAGCTTTAGGAGGAGGAAACAGCAAGAAAGTGCAACTGATACTGCATGTGTCACAGGGACCCAAGTTTGAGGATATATCAAATATCATGGAATCTCAACATATTTTTGCATTATGCTCTCTGATTGGTGCCGTTCAAAGGTGTACAGGCCTCTGGCGACTGCAGGAGGTTTTAGGTCAGTATAATTGGCCCGAATCACTTGACTCTGATTTTGTTTATGGCGCATCCTCAATTGGGTCAATCAATGCCAAATTTTTAGCTGCATTTTCAGTAGCTGCAGGAAAGAAATCATTGGAATTCGAGTCTGAAGAGTCCGATCCAGAGTGGGGCTGTTGGAGTGCTAGTCAAGAATCAAAAAGTCCATCCATTAAAATTGTTTTTCCTACCATAAAAAGAGTGAAGAATGCATGCAATGGAATCTTTCCTTCAAAACGCATTCTTTGCTTCTCTGAGAAAACATGGCAAAGGCTGAGGACTATAGACATACTTCATGATGCAATTCCTCACCCCTACGATAGAGTAGGACATCCAATGCACAGCAAGGTGGCACGGAGACGCTTCCACTCAAAGACAGACGGATCTTCATTTGGTTGGGTATATTGTGGTTCCCATAATTTCAGTGCAGCGGCCTGGGGACGACCAATCAATGGTCCATTTGGTTTAAACATGAACGGACTAGGGAAAGCCAACTCTACTTTGGGTCAGATGCTTCATATTTGCAACTATGAACTTGGGATCATTTTCACCTTTCCCCCAACAGACACAGATGGTAGCGCCAACAAAAATAGCGCAAAATTGGATAATATTGTTTTGCCATTCATTGTGCCTGCTCCAAAATACAGGCGAGGAGACAGGCCAGCAACAGGGAAGGCTATGAGGGAGGCATTAGCCGAACTTACTGAACAAGAGAGACAAAAACTTTTAGAAGCAGCTACAATCGAGGAAATTATGGAAGAGACTCCAGACGAAGATGAAGTAGTTGAGGCGGATGATTATGTTGCAGAAGAGAAGGAAGAGGAGAAGGCTTACGCCAAGATACTTTGGAGCCAAGTAGACTCATCCGAGAGCAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FHA HIRAN Tyr-DNA_phospho

Protein Analysis

1088

Amino Acids

120.16

Weight (kDa)

6.86

Isoelectric Point (pI)

49.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FHA	PF00498	64 - 154	4.4e-11	FHA domain
Tyr-DNA_phospho	PF06087	379 - 611	2e-24	Tyrosyl-DNA phosphodiesterase
HIRAN	PF08797	624 - 726	2.1e-09	HIRAN domain
Tyr-DNA_phospho	PF06087	778 - 1010	6e-23	Tyrosyl-DNA phosphodiesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006613)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07400 AT5G07400 AT5G07400 AT5G07400 AT5G07400 AT5G07400 AT5G07400 AT5G07400
fragaria_vesca	FvH4_5g10030
malus_domestica	MD06G1128300.v1.1 MD09G1063600.v1.1
prunus_persica	Prupe.5G138100_v2.0.a1
pyrus_communis	pycom06g11850
rosa_chinensis	RchiOBHm_Chr7g0188231
rosa_laevigata	RLG00000002473 RLG00000004716
rosa_multiflora	Rmu_sc0008750.1_g000014
rosa_roxburghii	Rroxscaffold_3G00266590
rosa_rugosa	Rorug06G0489000 Rorug06G0489300
rosa_samantha	Rh7AG093600 Rh7BG095000 Rh7CG095400 Rh7DG095500 Rh7DG095600
rosa_wichuraiana	Rw7G008060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	273, 1794
AatII	GACGTC	1 cut(s)	126
AccB1I	GGYRCC	2 cut(s)	42, 2318
AccB7I	CCANNNNNTGG	1 cut(s)	2837
AccI	GTMKAC	1 cut(s)	3243
AccIII	TCCGGA	1 cut(s)	139
AciI	CCGC	9 cut(s)	55, 71, 95, 202, 425, 809, 1426, 2816, 3176
AclWI	GGATC	5 cut(s)	189, 1879, 2501, 2776, 2933
AcsI	RAATTY	4 cut(s)	1513, 1862, 2441, 2486
AcuI	CTGAAG	4 cut(s)	870, 903, 2134, 2517
AcvI	CACGTG	2 cut(s)	109, 135
AcyI	GRCGYC	1 cut(s)	123
AfaI	GTAC	4 cut(s)	91, 540, 1698, 2335
AfiI	CCNNNNNNNGG	9 cut(s)	146, 628, 815, 1621, 2328, 2424, 2516, 2837, 3053
AflII	CTTAAG	2 cut(s)	670, 1943
AflIII	ACRYGT	1 cut(s)	134
AgeI	ACCGGT	1 cut(s)	146
AjnI	CCWGG	5 cut(s)	784, 1096, 1777, 1817, 2819
AloI	GAACNNNNNNTCC	2 cut(s)	2067, 2099
AlwI	GGATC	5 cut(s)	189, 1879, 2501, 2776, 2933
AlwNI	CAGNNNCTG	1 cut(s)	2465
Ama87I	CYCGRG	3 cut(s)	621, 1181, 1400
Aor13HI	TCCGGA	1 cut(s)	139
AoxI	GGCC	7 cut(s)	1135, 1404, 1707, 2338, 2378, 2817, 3044
ApeKI	GCWGC	6 cut(s)	115, 2192, 2450, 2465, 2813, 3119
ApoI	RAATTY	4 cut(s)	1513, 1862, 2441, 2486
ArsI	GACNNNNNNTTYG	4 cut(s)	1505, 1537, 2384, 2416
AsiGI	ACCGGT	1 cut(s)	146
Asp700I	GAANNNNTTC	1 cut(s)	1348
AspLEI	GCGC	5 cut(s)	311, 1583, 2414, 2966, 2981
AspS9I	GGNCC	9 cut(s)	103, 529, 563, 1135, 1468, 1708, 2249, 2379, 2840
AsuC2I	CCSGG	4 cut(s)	449, 622, 623, 1072
AsuHPI	GGTGA	4 cut(s)	44, 1344, 2687, 2926
AsuII	TTCGAA	1 cut(s)	721
AvaI	CYCGRG	3 cut(s)	621, 1181, 1400
AvaII	GGWCC	6 cut(s)	103, 529, 563, 1468, 2249, 2840
BaeGI	GKGCMC	1 cut(s)	47
BaeI	ACNNNNGTAYC	2 cut(s)	1680, 1713
BanI	GGYRCC	2 cut(s)	42, 2318
BbrPI	CACGTG	2 cut(s)	109, 135
BbsI	GAAGAC	3 cut(s)	220, 633, 1038
BbvCI	CCTCAGC	2 cut(s)	627, 2660
BbvI	GCAGC	6 cut(s)	127, 2204, 2437, 2452, 2825, 3131
BccI	CCATC	8 cut(s)	434, 983, 1043, 1287, 1458, 1613, 2560, 2951
BceAI	ACGGC	4 cut(s)	108, 225, 322, 2306
BcgI	CGANNNNNNTGC	2 cut(s)	300, 334
BciT130I	CCWGG	5 cut(s)	786, 1098, 1779, 1819, 2821
BciVI	GTATCC	1 cut(s)	1398
BclI	TGATCA	1 cut(s)	1837
BcnI	CCSGG	4 cut(s)	449, 622, 623, 1072
BfaI	CTAG	5 cut(s)	1275, 1701, 2103, 2532, 2867
BfmI	CTRYAG	5 cut(s)	1887, 2028, 2351, 2466, 2667
BfoI	RGCGCY	1 cut(s)	2967
BfrI	CTTAAG	2 cut(s)	670, 1943
BfuI	GTATCC	1 cut(s)	1398
BisI	GCNGC	7 cut(s)	116, 2193, 2451, 2466, 2814, 2817, 3120
BlpI	GCTNAGC	1 cut(s)	695
BlsI	GCNGC	7 cut(s)	117, 2194, 2452, 2467, 2815, 2818, 3121
Bme1390I	CCNGG	9 cut(s)	449, 622, 623, 786, 1072, 1098, 1779, 1819, 2821
Bme18I	GGWCC	6 cut(s)	103, 529, 563, 1468, 2249, 2840
BmeT110I	CYCGRG	3 cut(s)	621, 1181, 1400
BmgT120I	GGNCC	9 cut(s)	103, 529, 563, 1135, 1468, 1708, 2249, 2379, 2840
BmiI	GGNNCC	8 cut(s)	44, 324, 565, 2250, 2251, 2320, 2797, 3236
BmrFI	CCNGG	9 cut(s)	449, 622, 623, 786, 1072, 1098, 1779, 1819, 2821
BmrI	ACTGGG	1 cut(s)	1705
BmsI	GCATC	6 cut(s)	904, 1892, 2023, 2423, 2674, 2886
BmuI	ACTGGG	1 cut(s)	1705
BpiI	GAAGAC	3 cut(s)	220, 633, 1038
BplI	GAGNNNNNCTC	2 cut(s)	2063, 2095
BpmI	CTGGAG	3 cut(s)	768, 1800, 3135
Bpu10I	CCTNAGC	2 cut(s)	627, 2660
Bpu1102I	GCTNAGC	1 cut(s)	695
Bpu14I	TTCGAA	1 cut(s)	721
BpuEI	CTTGAG	2 cut(s)	945, 1019
BpuMI	CCSGG	4 cut(s)	449, 622, 623, 1072
Bsa29I	ATCGAT	1 cut(s)	340
BsaAI	YACGTR	2 cut(s)	109, 135
BsaBI	GATNNNNATC	1 cut(s)	993
BsaHI	GRCGYC	1 cut(s)	123
BsaI	GGTCTC	5 cut(s)	61, 438, 457, 794, 2128
BsaJI	CCNNGG	6 cut(s)	559, 593, 621, 1401, 1867, 2820
BsaWI	WCCGGW	4 cut(s)	139, 146, 154, 178
Bsc4I	CCNNNNNNNGG	9 cut(s)	146, 628, 815, 1621, 2328, 2424, 2516, 2837, 3053
Bse118I	RCCGGY	1 cut(s)	146
Bse1I	ACTGG	3 cut(s)	1286, 1711, 1759
Bse3DI	GCAATG	5 cut(s)	256, 706, 1686, 1921, 2608
Bse8I	GATNNNNATC	1 cut(s)	993
BseAI	TCCGGA	1 cut(s)	139
BseBI	CCWGG	5 cut(s)	786, 1098, 1779, 1819, 2821
BseCI	ATCGAT	1 cut(s)	340
BseDI	CCNNGG	6 cut(s)	559, 593, 621, 1401, 1867, 2820
BseGI	GGATG	9 cut(s)	14, 445, 1259, 1453, 2414, 2552, 2716, 3184, 3248
BseJI	GATNNNNATC	1 cut(s)	993
BseLI	CCNNNNNNNGG	9 cut(s)	146, 628, 815, 1621, 2328, 2424, 2516, 2837, 3053
BseMI	GCAATG	5 cut(s)	256, 706, 1686, 1921, 2608
BseMII	CTCAG	7 cut(s)	462, 618, 963, 1673, 1955, 2631, 2651
BseNI	ACTGG	3 cut(s)	1286, 1711, 1759
BseRI	GAGGAG	4 cut(s)	42, 2217, 3051, 3221
BseSI	GKGCMC	1 cut(s)	47
BseXI	GCAGC	6 cut(s)	127, 2204, 2437, 2452, 2825, 3131
BsgI	GTGCAG	3 cut(s)	700, 1096, 2832
Bsh1285I	CGRYCG	4 cut(s)	65, 159, 198, 238
BshFI	GGCC	7 cut(s)	1137, 1406, 1709, 2340, 2380, 2819, 3046
BshNI	GGYRCC	2 cut(s)	42, 2318
BshTI	ACCGGT	1 cut(s)	146
BshVI	ATCGAT	1 cut(s)	340
BsiEI	CGRYCG	4 cut(s)	65, 159, 198, 238
BsiHKCI	CYCGRG	3 cut(s)	621, 1181, 1400
BsiSI	CCGG	7 cut(s)	140, 147, 155, 179, 448, 622, 1072
BslFI	GGGAC	4 cut(s)	110, 612, 2262, 2838
BslI	CCNNNNNNNGG	9 cut(s)	146, 628, 815, 1621, 2328, 2424, 2516, 2837, 3053
BsmBI	CGTCTC	1 cut(s)	2737
BsmFI	GGGAC	4 cut(s)	110, 612, 2262, 2838
BsmI	GAATGC	3 cut(s)	1540, 2599, 2625
BsnI	GGCC	7 cut(s)	1137, 1406, 1709, 2340, 2380, 2819, 3046
Bso31I	GGTCTC	5 cut(s)	61, 438, 457, 794, 2128
BsoBI	CYCGRG	3 cut(s)	621, 1181, 1400
Bsp119I	TTCGAA	1 cut(s)	721
Bsp1286I	GDGCHC	1 cut(s)	47
Bsp13I	TCCGGA	1 cut(s)	139
Bsp1407I	TGTACA	2 cut(s)	538, 2333
Bsp143I	GATC	9 cut(s)	181, 195, 235, 283, 1837, 1871, 2506, 2768, 2925
Bsp1720I	GCTNAGC	1 cut(s)	695
Bsp19I	CCATGG	1 cut(s)	593
BspACI	CCGC	9 cut(s)	55, 71, 95, 202, 425, 809, 1426, 2816, 3176
BspANI	GGCC	7 cut(s)	1137, 1406, 1709, 2340, 2380, 2819, 3046
BspCNI	CTCAG	7 cut(s)	463, 619, 964, 1672, 1956, 2632, 2652
BspDI	ATCGAT	1 cut(s)	340
BspEI	TCCGGA	1 cut(s)	139
BspHI	TCATGA	1 cut(s)	2680
BspLI	GGNNCC	8 cut(s)	44, 324, 565, 2250, 2251, 2320, 2797, 3236
BspMAI	CTGCAG	3 cut(s)	2032, 2355, 2470
BspPI	GGATC	5 cut(s)	189, 1879, 2501, 2776, 2933
BspQI	GCTCTTC	1 cut(s)	649
BspT104I	TTCGAA	1 cut(s)	721
BspT107I	GGYRCC	2 cut(s)	42, 2318
BspTI	CTTAAG	2 cut(s)	670, 1943
BspTNI	GGTCTC	5 cut(s)	61, 438, 457, 794, 2128
BsrDI	GCAATG	5 cut(s)	256, 706, 1686, 1921, 2608
BsrFI	RCCGGY	1 cut(s)	146
BsrGI	TGTACA	2 cut(s)	538, 2333
BsrI	ACTGG	3 cut(s)	1286, 1711, 1759
BssAI	RCCGGY	1 cut(s)	146
BssECI	CCNNGG	6 cut(s)	559, 593, 621, 1401, 1867, 2820
BssMI	GATC	9 cut(s)	181, 195, 235, 283, 1837, 1871, 2506, 2768, 2925
BssNI	GRCGYC	1 cut(s)	123
BssT1I	CCWWGG	3 cut(s)	559, 593, 1867
Bst2UI	CCWGG	5 cut(s)	786, 1098, 1779, 1819, 2821
Bst4CI	ACNGT	6 cut(s)	42, 294, 1294, 1530, 1552, 2133
Bst6I	CTCTTC	8 cut(s)	348, 649, 1150, 2153, 2493, 3138, 3189, 3198
BstACI	GRCGYC	1 cut(s)	123
BstAFI	CTTAAG	2 cut(s)	670, 1943
BstAPI	GCANNNNNTGC	2 cut(s)	691, 2631
BstAUI	TGTACA	2 cut(s)	538, 2333
BstBAI	YACGTR	2 cut(s)	109, 135
BstBI	TTCGAA	1 cut(s)	721
BstC8I	GCNNGC	8 cut(s)	59, 683, 1447, 1665, 1833, 2599, 3018, 3048
BstDEI	CTNAG	9 cut(s)	471, 627, 695, 972, 1659, 1898, 1964, 2640, 2660
BstDSI	CCRYGG	1 cut(s)	593
BstF5I	GGATG	9 cut(s)	14, 445, 1259, 1453, 2414, 2552, 2716, 3184, 3248
BstH2I	RGCGCY	1 cut(s)	2967
BstHHI	GCGC	5 cut(s)	311, 1583, 2414, 2966, 2981
BstKTI	GATC	9 cut(s)	184, 198, 238, 286, 1840, 1874, 2509, 2771, 2928
BstMBI	GATC	9 cut(s)	181, 195, 235, 283, 1837, 1871, 2506, 2768, 2925
BstMCI	CGRYCG	4 cut(s)	65, 159, 198, 238
BstMWI	GCNNNNNNNGC	6 cut(s)	92, 623, 691, 1828, 2189, 2631
BstNI	CCWGG	5 cut(s)	786, 1098, 1779, 1819, 2821
BstNSI	RCATGY	3 cut(s)	1985, 2240, 2601
BstSCI	CCNGG	9 cut(s)	447, 620, 621, 784, 1070, 1096, 1777, 1817, 2819
BstSFI	CTRYAG	5 cut(s)	1887, 2028, 2351, 2466, 2667
BstSLI	GKGCMC	1 cut(s)	47
BstV1I	GCAGC	6 cut(s)	127, 2204, 2437, 2452, 2825, 3131
BstV2I	GAAGAC	3 cut(s)	220, 633, 1038
BstX2I	RGATCY	2 cut(s)	1871, 2768
BstYI	RGATCY	2 cut(s)	1871, 2768
Bsu15I	ATCGAT	1 cut(s)	340
BsuI	GTATCC	1 cut(s)	1398
BsuRI	GGCC	7 cut(s)	1137, 1406, 1709, 2340, 2380, 2819, 3046
BsuTUI	ATCGAT	1 cut(s)	340
BtgI	CCRYGG	1 cut(s)	593
BtgZI	GCGATG	1 cut(s)	873
BtsCI	GGATG	9 cut(s)	14, 445, 1259, 1453, 2414, 2552, 2716, 3184, 3248
BtsI	GCAGTG	2 cut(s)	1545, 2049
BtsIMutI	CAGTG	7 cut(s)	905, 1535, 1545, 1718, 2049, 2129, 2815
Cac8I	GCNNGC	8 cut(s)	59, 683, 1447, 1665, 1833, 2599, 3018, 3048
CaiI	CAGNNNCTG	1 cut(s)	2465
CciI	TCATGA	1 cut(s)	2680
CfoI	GCGC	5 cut(s)	311, 1583, 2414, 2966, 2981
Cfr10I	RCCGGY	1 cut(s)	146
Cfr13I	GGNCC	9 cut(s)	103, 529, 563, 1135, 1468, 1708, 2249, 2379, 2840
Cfr9I	CCCGGG	1 cut(s)	621
ClaI	ATCGAT	1 cut(s)	340
CseI	GACGC	2 cut(s)	1836, 2754
Csp6I	GTAC	4 cut(s)	90, 539, 1697, 2334
CspAI	ACCGGT	1 cut(s)	146
CviQI	GTAC	4 cut(s)	90, 539, 1697, 2334
DdeI	CTNAG	9 cut(s)	471, 627, 695, 972, 1659, 1898, 1964, 2640, 2660
DpnI	GATC	9 cut(s)	183, 197, 237, 285, 1839, 1873, 2508, 2770, 2927
DpnII	GATC	9 cut(s)	181, 195, 235, 283, 1837, 1871, 2506, 2768, 2925
DraI	TTTAAA	1 cut(s)	2853
Eam1104I	CTCTTC	8 cut(s)	348, 649, 1150, 2153, 2493, 3138, 3189, 3198
EarI	CTCTTC	8 cut(s)	348, 649, 1150, 2153, 2493, 3138, 3189, 3198
EciI	GGCGGA	2 cut(s)	217, 3191
Eco130I	CCWWGG	3 cut(s)	559, 593, 1867
Eco147I	AGGCCT	1 cut(s)	2340
Eco31I	GGTCTC	5 cut(s)	61, 438, 457, 794, 2128
Eco47I	GGWCC	6 cut(s)	103, 529, 563, 1468, 2249, 2840
Eco57I	CTGAAG	4 cut(s)	870, 903, 2134, 2517
Eco72I	CACGTG	2 cut(s)	109, 135
Eco88I	CYCGRG	3 cut(s)	621, 1181, 1400
EcoO109I	RGGNCCY	2 cut(s)	563, 2249
EcoRI	GAATTC	1 cut(s)	2486
EcoRII	CCWGG	5 cut(s)	784, 1096, 1777, 1817, 2819
EcoT14I	CCWWGG	3 cut(s)	559, 593, 1867
EcoT22I	ATGCAT	2 cut(s)	897, 2599
ErhI	CCWWGG	3 cut(s)	559, 593, 1867
Esp3I	CGTCTC	1 cut(s)	2737
FalI	AAGNNNNNCTT	4 cut(s)	1333, 1365, 2169, 2201
FaqI	GGGAC	4 cut(s)	110, 612, 2262, 2838
FbaI	TGATCA	1 cut(s)	1837
FblI	GTMKAC	1 cut(s)	3243
Fnu4HI	GCNGC	7 cut(s)	116, 2193, 2451, 2466, 2814, 2817, 3120
FokI	GGATG	8 cut(s)	452, 1246, 1440, 2401, 2539, 2703, 3191, 3235
Fsp4HI	GCNGC	7 cut(s)	116, 2193, 2451, 2466, 2814, 2817, 3120
FspBI	CTAG	5 cut(s)	1275, 1701, 2103, 2532, 2867
GlaI	GCGC	5 cut(s)	310, 1582, 2413, 2965, 2980
GluI	GCNGC	7 cut(s)	116, 2193, 2451, 2466, 2814, 2817, 3120
GsuI	CTGGAG	3 cut(s)	768, 1800, 3135
HaeII	RGCGCY	1 cut(s)	2967
HaeIII	GGCC	7 cut(s)	1137, 1406, 1709, 2340, 2380, 2819, 3046
HapII	CCGG	7 cut(s)	140, 147, 155, 179, 448, 622, 1072
HgaI	GACGC	2 cut(s)	1836, 2754
HhaI	GCGC	5 cut(s)	311, 1583, 2414, 2966, 2981
Hin1I	GRCGYC	1 cut(s)	123
Hin6I	GCGC	5 cut(s)	309, 1581, 2412, 2964, 2979
HinP1I	GCGC	5 cut(s)	309, 1581, 2412, 2964, 2979
HincII	GTYRAC	2 cut(s)	1524, 1723
HindII	GTYRAC	2 cut(s)	1524, 1723
HindIII	AAGCTT	2 cut(s)	1940, 1989
HpaII	CCGG	7 cut(s)	140, 147, 155, 179, 448, 622, 1072
HphI	GGTGA	4 cut(s)	44, 1344, 2687, 2926
Hpy166II	GTNNAC	7 cut(s)	106, 882, 1524, 1723, 1979, 2334, 3244
Hpy8I	GTNNAC	7 cut(s)	106, 882, 1524, 1723, 1979, 2334, 3244
Hpy99I	CGWCG	1 cut(s)	125
HpyAV	CCTTC	5 cut(s)	410, 2625, 3052, 3193, 3205
HpyCH4III	ACNGT	6 cut(s)	42, 294, 1294, 1530, 1552, 2133
HpyCH4IV	ACGT	4 cut(s)	108, 123, 134, 601
HpyF10VI	GCNNNNNNNGC	6 cut(s)	92, 623, 691, 1828, 2189, 2631
HpyF3I	CTNAG	9 cut(s)	471, 627, 695, 972, 1659, 1898, 1964, 2640, 2660
HpySE526I	ACGT	4 cut(s)	108, 123, 134, 601
Hsp92I	GRCGYC	1 cut(s)	123
HspAI	GCGC	5 cut(s)	309, 1581, 2412, 2964, 2979
KflI	GGGWCCC	1 cut(s)	2249
Kpn2I	TCCGGA	1 cut(s)	139
Ksp22I	TGATCA	1 cut(s)	1837
Kzo9I	GATC	9 cut(s)	181, 195, 235, 283, 1837, 1871, 2506, 2768, 2925
LguI	GCTCTTC	1 cut(s)	649
LmnI	GCTCC	3 cut(s)	204, 3025, 3234
Lsp1109I	GCAGC	6 cut(s)	127, 2204, 2437, 2452, 2825, 3131
LweI	GCATC	6 cut(s)	904, 1892, 2023, 2423, 2674, 2886
MaeI	CTAG	5 cut(s)	1275, 1701, 2103, 2532, 2867
MaeII	ACGT	4 cut(s)	108, 123, 134, 601
MaeIII	GTNAC	4 cut(s)	1238, 1288, 1540, 2241
MalI	GATC	9 cut(s)	183, 197, 237, 285, 1839, 1873, 2508, 2770, 2927
MboI	GATC	9 cut(s)	181, 195, 235, 283, 1837, 1871, 2506, 2768, 2925
MfeI	CAATTG	2 cut(s)	1998, 2421
MflI	RGATCY	2 cut(s)	1871, 2768
MhlI	GDGCHC	1 cut(s)	47
MmeI	TCCRAC	6 cut(s)	199, 417, 631, 1301, 1791, 2504
Mph1103I	ATGCAT	2 cut(s)	897, 2599
MroI	TCCGGA	1 cut(s)	139
MroXI	GAANNNNTTC	1 cut(s)	1348
MseI	TTAA	8 cut(s)	510, 570, 671, 813, 1944, 2006, 2559, 2852
MslI	CAYNNNNRTG	3 cut(s)	592, 832, 900
MspA1I	CMGCKG	2 cut(s)	1663, 2816
MspCI	CTTAAG	2 cut(s)	670, 1943
MspI	CCGG	7 cut(s)	140, 147, 155, 179, 448, 622, 1072
MspR9I	CCNGG	9 cut(s)	449, 622, 623, 786, 1072, 1098, 1779, 1819, 2821
MssI	GTTTAAAC	1 cut(s)	2853
MunI	CAATTG	2 cut(s)	1998, 2421
Mva1269I	GAATGC	3 cut(s)	1540, 2599, 2625
MvaI	CCWGG	5 cut(s)	786, 1098, 1779, 1819, 2821
MwoI	GCNNNNNNNGC	6 cut(s)	92, 623, 691, 1828, 2189, 2631
NciI	CCSGG	4 cut(s)	449, 622, 623, 1072
NcoI	CCATGG	1 cut(s)	593
NdeII	GATC	9 cut(s)	181, 195, 235, 283, 1837, 1871, 2506, 2768, 2925
NlaIV	GGNNCC	8 cut(s)	44, 324, 565, 2250, 2251, 2320, 2797, 3236
NmeAIII	GCCGAG	1 cut(s)	99
NmuCI	GTSAC	3 cut(s)	1238, 1540, 2241
NsiI	ATGCAT	2 cut(s)	897, 2599
NspI	RCATGY	3 cut(s)	1985, 2240, 2601
NspV	TTCGAA	1 cut(s)	721
PaeI	GCATGC	1 cut(s)	2601
PagI	TCATGA	1 cut(s)	2680
PceI	AGGCCT	1 cut(s)	2340
PciSI	GCTCTTC	1 cut(s)	649
PcsI	WCGNNNNNNNCGW	1 cut(s)	231
PctI	GAATGC	3 cut(s)	1540, 2599, 2625
PdmI	GAANNNNTTC	1 cut(s)	1348
PflFI	GACNNNGTC	1 cut(s)	449
PflMI	CCANNNNNTGG	1 cut(s)	2837
PfoI	TCCNGGA	1 cut(s)	1777
PinAI	ACCGGT	1 cut(s)	146
PkrI	GCNGC	7 cut(s)	117, 2194, 2452, 2467, 2815, 2818, 3121
Ple19I	CGATCG	2 cut(s)	198, 238
PmaCI	CACGTG	2 cut(s)	109, 135
PmeI	GTTTAAAC	1 cut(s)	2853
PmlI	CACGTG	2 cut(s)	109, 135
Ppu21I	YACGTR	2 cut(s)	109, 135
PpuMI	RGGWCCY	2 cut(s)	563, 2249
PsiI	TTATAA	2 cut(s)	273, 1794
Psp5II	RGGWCCY	2 cut(s)	563, 2249
Psp6I	CCWGG	5 cut(s)	784, 1096, 1777, 1817, 2819
PspCI	CACGTG	2 cut(s)	109, 135
PspGI	CCWGG	5 cut(s)	784, 1096, 1777, 1817, 2819
PspN4I	GGNNCC	8 cut(s)	44, 324, 565, 2250, 2251, 2320, 2797, 3236
PspPI	GGNCC	9 cut(s)	103, 529, 563, 1135, 1468, 1708, 2249, 2379, 2840
PspPPI	RGGWCCY	2 cut(s)	563, 2249
PstI	CTGCAG	3 cut(s)	2032, 2355, 2470
PstNI	CAGNNNCTG	1 cut(s)	2465
PsuI	RGATCY	2 cut(s)	1871, 2768
PsyI	GACNNNGTC	1 cut(s)	449
PvuI	CGATCG	2 cut(s)	198, 238
PvuII	CAGCTG	1 cut(s)	1663
RsaI	GTAC	4 cut(s)	91, 540, 1698, 2335
RsaNI	GTAC	4 cut(s)	90, 539, 1697, 2334
RseI	CAYNNNNRTG	3 cut(s)	592, 832, 900
SapI	GCTCTTC	1 cut(s)	649
SaqAI	TTAA	8 cut(s)	510, 570, 671, 813, 1944, 2006, 2559, 2852
SatI	GCNGC	7 cut(s)	116, 2193, 2451, 2466, 2814, 2817, 3120
Sau3AI	GATC	9 cut(s)	181, 195, 235, 283, 1837, 1871, 2506, 2768, 2925
Sau96I	GGNCC	9 cut(s)	103, 529, 563, 1135, 1468, 1708, 2249, 2379, 2840
ScrFI	CCNGG	9 cut(s)	449, 622, 623, 786, 1072, 1098, 1779, 1819, 2821
SduI	GDGCHC	1 cut(s)	47
SfaNI	GCATC	6 cut(s)	904, 1892, 2023, 2423, 2674, 2886
SfcI	CTRYAG	5 cut(s)	1887, 2028, 2351, 2466, 2667
SfuI	TTCGAA	1 cut(s)	721
SinI	GGWCC	6 cut(s)	103, 529, 563, 1468, 2249, 2840
SmaI	CCCGGG	1 cut(s)	623
SmiMI	CAYNNNNRTG	3 cut(s)	592, 832, 900
SmlI	CTYRAG	4 cut(s)	670, 960, 998, 1943
SmoI	CTYRAG	4 cut(s)	670, 960, 998, 1943
SphI	GCATGC	1 cut(s)	2601
SseBI	AGGCCT	1 cut(s)	2340
SsiI	CCGC	9 cut(s)	55, 71, 95, 202, 425, 809, 1426, 2816, 3176
SspI	AATATT	2 cut(s)	2050, 2995
SspMI	CTAG	5 cut(s)	1275, 1701, 2103, 2532, 2867
StuI	AGGCCT	1 cut(s)	2340
StyD4I	CCNGG	9 cut(s)	447, 620, 621, 784, 1070, 1096, 1777, 1817, 2819
StyI	CCWWGG	3 cut(s)	559, 593, 1867
TaaI	ACNGT	6 cut(s)	42, 294, 1294, 1530, 1552, 2133
TaiI	ACGT	4 cut(s)	111, 126, 137, 604
TaqI	TCGA	7 cut(s)	120, 159, 340, 721, 792, 2490, 3129
TaqII	GACCGA	2 cut(s)	79, 91
TatI	WGTACW	2 cut(s)	538, 2333
TauI	GCSGC	1 cut(s)	2819
Tru1I	TTAA	8 cut(s)	510, 570, 671, 813, 1944, 2006, 2559, 2852
Tru9I	TTAA	8 cut(s)	510, 570, 671, 813, 1944, 2006, 2559, 2852
TscAI	CASTG	7 cut(s)	905, 1535, 1545, 1718, 2049, 2136, 2815
TseFI	GTSAC	3 cut(s)	1238, 1540, 2241
TseI	GCWGC	6 cut(s)	115, 2192, 2450, 2465, 2813, 3119
Tsp45I	GTSAC	3 cut(s)	1238, 1540, 2241
TspGWI	ACGGA	6 cut(s)	39, 627, 1156, 2755, 2781, 2877
TspMI	CCCGGG	1 cut(s)	621
TspRI	CASTG	7 cut(s)	905, 1535, 1545, 1718, 2049, 2136, 2815
Tth111I	GACNNNGTC	1 cut(s)	449
Van91I	CCANNNNNTGG	1 cut(s)	2837
Vha464I	CTTAAG	2 cut(s)	670, 1943
VpaK11BI	GGWCC	6 cut(s)	103, 529, 563, 1468, 2249, 2840
XapI	RAATTY	4 cut(s)	1513, 1862, 2441, 2486
XceI	RCATGY	3 cut(s)	1985, 2240, 2601
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2884
XmaI	CCCGGG	1 cut(s)	621
XmiI	GTMKAC	1 cut(s)	3243
XmnI	GAANNNNTTC	1 cut(s)	1348
XspI	CTAG	5 cut(s)	1275, 1701, 2103, 2532, 2867
ZraI	GACGTC	1 cut(s)	124
Zsp2I	ATGCAT	2 cut(s)	897, 2599

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.