Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0003328

Physical Location & Seq

Genomic Coordinates Reverse (-)

27456418 .. 27464031

Gene Structure 7614 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1131000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2919 bp

ATGCCATTTGAAATAATGGATCAAAGGGGTGTATCTGTCACTTCCCACTTCTTTGATGAAATCCCTTTTCCTGCTGAGAGACAAGTCGGATTTCAGAAGCTGAAAACCATGTCCGATTACCAAGCGGGAGTCGGTGGAATGGTACCGACACCTGGCAGCAAGTTGGGATCTTCAATGCCATCTAAAATGTATTTACCAAATGGGGCACAGTCGGTGGATCACTTGGATCTGCCACAATCCAATCTGGCTGGGGAGCAAACAGAAAGACTAAGCATCGGTGGAGGGGTGCCCAACATCTCCAAGGCCTCGTGGAAACCTATGGATCACCAACCAAAATTGTGGTCAGACTTGTCCGCAAAGCCAACACCTTATAGCTCGGTTGGTGGCAAAACTGTTATTAATGGCGCCCATCATGAAAGTAGCCTGTTCTCAAGCTCTTTGTCTGAAATTTTTAGCAGAAAGTTGCGATTATTGAGGAGTGATTTTGTTTCTCATCAATCTACTAACAACGGTGCTTACCAACATGAGGAAGAGCCCTTTGAATCTCTTGAAGAAATTGAGGCACAAACAATTGGAAATCTCCTTCCAGATGAAAATGACTTGTTTTCTGGCATGACAGATGAGCCGGGTTATAAGGCTCATGTTAATATTGGTGATGGATTTGAAGATTTTGATCTCTTTAGCAGTGGTGGTGGAATGGAATTGGAAGGCGATGATCAAGGGGTTTATAATAGCTCAATTGCGGGTGAACACCCTTTTGGAGAACACCCTTCCCGAACACTTTTTGTCCGCAATATTAATAGCAACATTGAAGATTTGGAATTGAAGGCTCTTTTTGAGCAATATGGAGACATCCGGGCACTTTACACAGCCTGCAAGCATCGTGGGTTTGTTATGATTTCTTATTATGATATAAGGGCTGCCCAAAATGCACTAAGAGCACTTCAAAGTAAGCCACTGAGGCGTAGGAAGCTTGACATACATTATTCGATACCCAAAGACAATCCTTCTGATAAAGATATTAACCAAGGAACGCTAGTGTTATTCAACCTTGATTCTTCTGTTTCTAATGACGAGCTTTCTAAAACTTTTGGATCTTATGGAGAAATTAAAGAAATCCGTGAGACTCCACACAAGCCACATAACAAGTTCATAGAGTTTTATGATGTTAGAGCTGCAGAAGCTGCTCTCCATGCATTGAATAGGAGTGAGATTGGAGGGAAGCAGATCAAGCTTGAACCCAGCCGTCCAGGGGGCACAAGGAGGGGTTTGGCTCAAGTTTCCGAGCAGGAGCAAGATGAATTCAAATTTTGTCATAGCTTGTCTGACAGTTTATCAACAGGCACCAAGCCAACACTTCATCCTGGAGTGGTCACATCAAGCTGCATGGTTAATGGATTTGGCCTGGGCTTACACTCTGCACTCCGTTCACCTGTGAGCACATTTATTGAAAACGCTTTATCTCATCAGAGTTCAAGTGTTCCAGATACCTTGCCCGCTCCAGTGACAGGGGCATCTATAGGGAAAAAATTTAGCCTCCGTGACCCCGATCAGTCTCTGGATGACATGAATTTTGGTAATCAGAGTGTTCCCGGCATACATCCTCATTCGCTTCCTGAGTATAATGACAGTTTAGCCCATGGTATTCCATGCAACACTGCTGGTGCTATCGGAAGCATGAATGGCAATCTGGGACTTAGAATGAGAGAAGGAACAGACAGAAGGTCCACTGTCAGTGCAAATGGTCACCTAGTGGAACTGAACGGAGGAGCTTTTGGATCTCCTGGAAATGGGAGCCGCCCGGTTCATGCTCATCCCTATGTTTTGAATAACTCCAACTTGTACCAGCAGCATCCTTCAAGTCCCATGATGTGGCAGAATTCACCATCATTTATCAGTGGTCCTACTGCGCATCACTTTCCCCAAATGCCAGGATTCCACAGGACACCACCTCATATGCTGAGCGGTACTTCGCCTGTGCACCACCATGTAGGATCAGCTCCGGCTGTTAACCCTTCCCTTTGGGAAAGGCATGCCTTTTCAGGGGAGTCTCCTGACACTTCTAGCCTTCACTTGGGTTCTCTTGGCTTTGCACGTTTTTCCGGTAGTCCCCAGATGCATCCTGTGGATGTCTCTTCACTCAACCTCTTCTCTCTTACTGGTGGAAATTTCTTGGACATGACTACCAGTACCCGGCAGCGCTCTTCACAGGAGATAGGCCACATGTTTCCCGGTAGAAATTCGATGAATTCAATGCCTTCCTCTTTTGATTCTCAAAACGAACGTGGTGTTAGAAGCCTGTCTCACCGAAGAAATGAAGCAAACTCTAACAATGCTGATAAGAGACAATATGAGCTTGATATTGAACGAATACTCCATGGGGAAGACAGACGCACCACATTGATGATAAAGAACATTCCCAACAAGTACACTTCAAAGATGCTTCTTGCTGCAATTGATGAGCAGTGTCGAGGAATTTATGACTTTCTTTATTTGCCAATCGACTTCAAGAACAAATGCAATGTGGGATACGCATTCATCAACATGATCGATCCTCATCAGATTGTTCCATTCTATAAGGCATTCAATGGAAAAAAATGGGAGAAGTTCAACAGTGAAAAGGTGGCATCACTTGCATATGCTCGTATTCAGGGAAAAGCTGCTCTTATTGCCCATTTTCAGAACTCAAGCTTAATGAACGAGGACAAACGCTGCCGTCCCATTCTCTTCCACACGGAGGGTCCAAATGCTGGTGATCCGGAACCCTTTCCCATGGGTACCAACATCCGGTCAAGACCAGGAAAACCTCGAACAGCTGGCTACGATGATAACCATCATCACGGGAGTCCTTCGGCAACTGCAAACGGAGAGGAAAAATCCAAGGAATCCGAGCAAGACTTGTTCTACCCAGATTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0008150 GO:0010564 GO:0010638 GO:0033043 GO:0040008 GO:0040020 GO:0045787 GO:0045836 GO:0045927 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051445 GO:0051446 GO:0051726 GO:0051783 GO:0051785 GO:0065007 GO:0090068 GO:0097159 GO:1901363 GO:2000241 GO:2000243

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RRM_1 RRM_2

Protein Analysis

973

Amino Acids

107.15

Weight (kDa)

6.24

Isoelectric Point (pI)

55.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RRM_1	PF00076	261 - 325	7.8e-10	RNA recognition motif
RRM_1	PF00076	347 - 411	3.2e-09	RNA recognition motif
RRM_2	PF04059	798 - 894	1e-54	RNA recognition motif 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003328)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07290 AT5G61960 AT5G61960 AT5G61960 AT5G61960 AT5G61960 AT5G61960 AT5G61960 AT5G61960 AT5G61960 AT5G61960 AT5G61960
fragaria_vesca	FvH4_5g09460
malus_domestica	MD06G1131000.v1.1 MD14G1147600.v1.1
prunus_persica	Prupe.5G142200_v2.0.a1 Prupe.5G142200_v2.0.a1 Prupe.5G142200_v2.0.a1 Prupe.5G142200_v2.0.a1 Prupe.5G142200_v2.0.a1
pyrus_communis	pycom06g12260 pycom14g12230
rosa_chinensis	RchiOBHm_Chr7g0188731
rosa_laevigata	RLG00000004666
rosa_multiflora	Rmu_sc0003854.1_g000006
rosa_roxburghii	Rroxscaffold_3G00266090
rosa_rugosa	Rorug06G0493200.1 Rorug06G0493300.1 Rorug06G0493400 Rorug06G0493400 Rorug06G0493500
rosa_samantha	Rh7AG098000 Rh7BG099400 Rh7CG101200 Rh7DG099800
rosa_wichuraiana	Rw7G008440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	633, 729
Acc16I	TGCGCA	1 cut(s)	1911
Acc65I	GGTACC	2 cut(s)	142, 2777
AccB1I	GGYRCC	5 cut(s)	142, 286, 404, 1343, 2777
AccB7I	CCANNNNNTGG	2 cut(s)	1872, 2750
AccBSI	CCGCTC	2 cut(s)	1499, 1965
AccIII	TCCGGA	1 cut(s)	2758
AciI	CCGC	7 cut(s)	125, 354, 743, 790, 1497, 1798, 1965
AcyI	GRCGYC	1 cut(s)	405
AdeI	CACNNNGTG	1 cut(s)	1753
AfaI	GTAC	6 cut(s)	144, 1844, 1969, 2191, 2429, 2779
AfeI	AGCGCT	1 cut(s)	2201
AflIII	ACRYGT	1 cut(s)	2223
AhdI	GACNNNNNGTC	1 cut(s)	1723
AjnI	CCWGG	7 cut(s)	151, 1249, 1363, 1404, 1783, 1930, 2797
AjuI	GAANNNNNNNTTGG	2 cut(s)	741, 773
AloI	GAACNNNNNNTCC	2 cut(s)	2358, 2390
Alw21I	GWGCWC	3 cut(s)	943, 1442, 1983
Alw26I	GTCTC	8 cut(s)	73, 843, 1118, 1560, 2055, 2137, 2307, 2338
Alw44I	GTGCAC	1 cut(s)	1979
AlwNI	CAGNNNCTG	3 cut(s)	100, 1184, 1558
Aor13HI	TCCGGA	1 cut(s)	2758
Aor51HI	AGCGCT	1 cut(s)	2201
AoxI	GGCC	3 cut(s)	303, 1402, 2218
ApaLI	GTGCAC	1 cut(s)	1979
ArsI	GACNNNNNNTTYG	4 cut(s)	326, 358, 1556, 1588
AseI	ATTAAT	2 cut(s)	399, 798
Asp700I	GAANNNNTTC	1 cut(s)	2766
Asp718I	GGTACC	2 cut(s)	142, 2777
AspLEI	GCGC	3 cut(s)	407, 1912, 2202
AspS9I	GGNCC	3 cut(s)	1725, 1901, 2741
AsuC2I	CCSGG	6 cut(s)	627, 857, 1593, 1802, 2194, 2232
AsuHPI	GGTGA	8 cut(s)	317, 665, 758, 1422, 1739, 1875, 2297, 2765
AvaII	GGWCC	3 cut(s)	1725, 1901, 2741
BaeGI	GKGCMC	5 cut(s)	208, 291, 862, 1259, 1983
BanI	GGYRCC	5 cut(s)	142, 286, 404, 1343, 2777
BanII	GRGCYC	1 cut(s)	537
BauI	CACGAG	1 cut(s)	307
BbsI	GAAGAC	1 cut(s)	2391
Bbv12I	GWGCWC	3 cut(s)	943, 1442, 1983
BccI	CCATC	5 cut(s)	187, 417, 650, 1894, 2841
BceAI	ACGGC	2 cut(s)	1230, 2700
BciT130I	CCWGG	7 cut(s)	153, 1251, 1365, 1406, 1785, 1932, 2799
BciVI	GTATCC	1 cut(s)	2522
BclI	TGATCA	1 cut(s)	715
BcnI	CCSGG	6 cut(s)	627, 857, 1593, 1802, 2194, 2232
BcoDI	GTCTC	8 cut(s)	73, 843, 1118, 1560, 2055, 2137, 2307, 2338
BfaI	CTAG	3 cut(s)	1037, 1751, 2064
BfmI	CTRYAG	2 cut(s)	1176, 1518
BfoI	RGCGCY	2 cut(s)	408, 2203
BfuI	GTATCC	1 cut(s)	2522
BglI	GCCNNNNNGGC	1 cut(s)	961
BlpI	GCTNAGC	1 cut(s)	1961
Bme18I	GGWCC	3 cut(s)	1725, 1901, 2741
BmeRI	GACNNNNNGTC	1 cut(s)	1723
BmgT120I	GGNCC	3 cut(s)	1725, 1901, 2741
BmiI	GGNNCC	8 cut(s)	144, 288, 406, 1345, 1796, 2742, 2763, 2779
BmsI	GCATC	9 cut(s)	282, 889, 1523, 1861, 1921, 2106, 2128, 2430, 2636
BpiI	GAAGAC	1 cut(s)	2391
BpmI	CTGGAG	2 cut(s)	1386, 1485
Bpu1102I	GCTNAGC	1 cut(s)	1961
BpuEI	CTTGAG	3 cut(s)	415, 1260, 2671
BpuMI	CCSGG	6 cut(s)	627, 857, 1593, 1802, 2194, 2232
Bsa29I	ATCGAT	1 cut(s)	2550
BsaBI	GATNNNNATC	3 cut(s)	672, 2556, 2832
BsaHI	GRCGYC	1 cut(s)	405
BsaJI	CCNNGG	8 cut(s)	300, 1027, 1250, 1405, 1639, 2377, 2772, 2880
BsaWI	WCCGGW	3 cut(s)	2102, 2758, 2787
BsaXI	ACNNNNNCTCC	2 cut(s)	2358, 2388
Bse1I	ACTGG	3 cut(s)	1502, 2164, 2187
Bse3DI	GCAATG	1 cut(s)	2527
Bse8I	GATNNNNATC	3 cut(s)	672, 2556, 2832
BseAI	TCCGGA	1 cut(s)	2758
BseBI	CCWGG	7 cut(s)	153, 1251, 1365, 1406, 1785, 1932, 2799
BseCI	ATCGAT	1 cut(s)	2550
BseDI	CCNNGG	8 cut(s)	300, 1027, 1250, 1405, 1639, 2377, 2772, 2880
BseGI	GGATG	9 cut(s)	852, 1360, 1567, 1600, 1813, 1852, 2119, 2134, 2784
BseJI	GATNNNNATC	3 cut(s)	672, 2556, 2832
BseMI	GCAATG	1 cut(s)	2527
BseMII	CTCAG	4 cut(s)	66, 950, 1608, 1952
BseNI	ACTGG	3 cut(s)	1502, 2164, 2187
BseRI	GAGGAG	2 cut(s)	490, 1782
BseSI	GKGCMC	5 cut(s)	208, 291, 862, 1259, 1983
BseYI	CCCAGC	2 cut(s)	248, 1241
BsgI	GTGCAG	1 cut(s)	1404
BshFI	GGCC	3 cut(s)	305, 1404, 2220
BshNI	GGYRCC	5 cut(s)	142, 286, 404, 1343, 2777
BshVI	ATCGAT	1 cut(s)	2550
BsiHKAI	GWGCWC	3 cut(s)	943, 1442, 1983
BslFI	GGGAC	4 cut(s)	1707, 1848, 2094, 2703
BsmAI	GTCTC	8 cut(s)	73, 843, 1118, 1560, 2055, 2137, 2307, 2338
BsmFI	GGGAC	4 cut(s)	1707, 1848, 2094, 2703
BsmI	GAATGC	2 cut(s)	2534, 2582
BsnI	GGCC	3 cut(s)	305, 1404, 2220
Bsp1286I	GDGCHC	8 cut(s)	208, 291, 537, 862, 943, 1259, 1442, 1983
Bsp13I	TCCGGA	1 cut(s)	2758
Bsp1720I	GCTNAGC	1 cut(s)	1961
Bsp19I	CCATGG	3 cut(s)	1639, 2377, 2772
BspACI	CCGC	7 cut(s)	125, 354, 743, 790, 1497, 1798, 1965
BspANI	GGCC	3 cut(s)	305, 1404, 2220
BspCNI	CTCAG	4 cut(s)	67, 951, 1609, 1953
BspDI	ATCGAT	1 cut(s)	2550
BspEI	TCCGGA	1 cut(s)	2758
BspHI	TCATGA	1 cut(s)	412
BspLI	GGNNCC	8 cut(s)	144, 288, 406, 1345, 1796, 2742, 2763, 2779
BspMAI	CTGCAG	1 cut(s)	1180
BspQI	GCTCTTC	2 cut(s)	525, 2209
BspT107I	GGYRCC	5 cut(s)	142, 286, 404, 1343, 2777
BsrBI	CCGCTC	2 cut(s)	1499, 1965
BsrDI	GCAATG	1 cut(s)	2527
BsrI	ACTGG	3 cut(s)	1502, 2164, 2187
BssECI	CCNNGG	8 cut(s)	300, 1027, 1250, 1405, 1639, 2377, 2772, 2880
BssNI	GRCGYC	1 cut(s)	405
BssSI	CACGAG	1 cut(s)	307
BssT1I	CCWWGG	6 cut(s)	300, 1027, 1639, 2377, 2772, 2880
Bst2BI	CACGAG	1 cut(s)	307
Bst2UI	CCWGG	7 cut(s)	153, 1251, 1365, 1406, 1785, 1932, 2799
Bst4CI	ACNGT	7 cut(s)	210, 394, 512, 1331, 1631, 1732, 2615
Bst6I	CTCTTC	5 cut(s)	525, 2140, 2153, 2209, 2732
BstACI	GRCGYC	1 cut(s)	405
BstAPI	GCANNNNNTGC	2 cut(s)	1184, 2633
BstC8I	GCNNGC	5 cut(s)	874, 878, 1497, 2034, 2818
BstDEI	CTNAG	7 cut(s)	75, 269, 935, 959, 1617, 1697, 1961
BstDSI	CCRYGG	3 cut(s)	1639, 2377, 2772
BstEII	GGTNACC	1 cut(s)	1745
BstF5I	GGATG	9 cut(s)	852, 1360, 1567, 1600, 1813, 1852, 2119, 2134, 2784
BstH2I	RGCGCY	2 cut(s)	408, 2203
BstHHI	GCGC	3 cut(s)	407, 1912, 2202
BstMAI	GTCTC	8 cut(s)	73, 843, 1118, 1560, 2055, 2137, 2307, 2338
BstNI	CCWGG	7 cut(s)	153, 1251, 1365, 1406, 1785, 1932, 2799
BstNSI	RCATGY	2 cut(s)	2036, 2227
BstPI	GGTNACC	1 cut(s)	1745
BstSFI	CTRYAG	2 cut(s)	1176, 1518
BstSLI	GKGCMC	5 cut(s)	208, 291, 862, 1259, 1983
BstV2I	GAAGAC	1 cut(s)	2391
BstX2I	RGATCY	4 cut(s)	167, 226, 1094, 1778
BstXI	CCANNNNNNTGG	1 cut(s)	339
BstYI	RGATCY	4 cut(s)	167, 226, 1094, 1778
Bsu15I	ATCGAT	1 cut(s)	2550
BsuI	GTATCC	1 cut(s)	2522
BsuRI	GGCC	3 cut(s)	305, 1404, 2220
BsuTUI	ATCGAT	1 cut(s)	2550
BtgI	CCRYGG	3 cut(s)	1639, 2377, 2772
BtgZI	GCGATG	1 cut(s)	726
BtsCI	GGATG	9 cut(s)	852, 1360, 1567, 1600, 1813, 1852, 2119, 2134, 2784
BtsI	GCAGTG	3 cut(s)	691, 1656, 2471
BtsIMutI	CAGTG	9 cut(s)	691, 956, 1509, 1656, 1728, 1741, 1903, 2471, 2620
Cac8I	GCNNGC	5 cut(s)	874, 878, 1497, 2034, 2818
CaiI	CAGNNNCTG	3 cut(s)	100, 1184, 1558
CciI	TCATGA	1 cut(s)	412
CfoI	GCGC	3 cut(s)	407, 1912, 2202
Cfr13I	GGNCC	3 cut(s)	1725, 1901, 2741
ClaI	ATCGAT	1 cut(s)	2550
CseI	GACGC	1 cut(s)	2400
Csp6I	GTAC	6 cut(s)	143, 1843, 1968, 2190, 2428, 2778
CspCI	CAANNNNNGTGG	2 cut(s)	865, 900
CviQI	GTAC	6 cut(s)	143, 1843, 1968, 2190, 2428, 2778
DdeI	CTNAG	7 cut(s)	75, 269, 935, 959, 1617, 1697, 1961
DinI	GGCGCC	1 cut(s)	406
DraIII	CACNNNGTG	1 cut(s)	1753
DriI	GACNNNNNGTC	1 cut(s)	1723
Eam1104I	CTCTTC	5 cut(s)	525, 2140, 2153, 2209, 2732
Eam1105I	GACNNNNNGTC	1 cut(s)	1723
EarI	CTCTTC	5 cut(s)	525, 2140, 2153, 2209, 2732
Eco130I	CCWWGG	6 cut(s)	300, 1027, 1639, 2377, 2772, 2880
Eco147I	AGGCCT	1 cut(s)	305
Eco24I	GRGCYC	1 cut(s)	537
Eco47I	GGWCC	3 cut(s)	1725, 1901, 2741
Eco47III	AGCGCT	1 cut(s)	2201
Eco91I	GGTNACC	1 cut(s)	1745
EcoO65I	GGTNACC	1 cut(s)	1745
EcoRI	GAATTC	3 cut(s)	1301, 1879, 2248
EcoRII	CCWGG	7 cut(s)	151, 1249, 1363, 1404, 1783, 1930, 2797
EcoT14I	CCWWGG	6 cut(s)	300, 1027, 1639, 2377, 2772, 2880
EcoT22I	ATGCAT	2 cut(s)	1198, 2121
EcoT38I	GRGCYC	1 cut(s)	537
EgeI	GGCGCC	1 cut(s)	406
EheI	GGCGCC	1 cut(s)	406
ErhI	CCWWGG	6 cut(s)	300, 1027, 1639, 2377, 2772, 2880
FalI	AAGNNNNNCTT	2 cut(s)	2649, 2681
FaqI	GGGAC	4 cut(s)	1707, 1848, 2094, 2703
FauI	CCCGC	3 cut(s)	118, 736, 1504
FauNDI	CATATG	2 cut(s)	1956, 2638
FbaI	TGATCA	1 cut(s)	715
FokI	GGATG	9 cut(s)	839, 1347, 1574, 1587, 1800, 1839, 2106, 2141, 2771
FriOI	GRGCYC	1 cut(s)	537
FspBI	CTAG	3 cut(s)	1037, 1751, 2064
FspI	TGCGCA	1 cut(s)	1911
GlaI	GCGC	3 cut(s)	406, 1911, 2201
GsaI	CCCAGC	2 cut(s)	252, 1245
GsuI	CTGGAG	2 cut(s)	1386, 1485
HaeII	RGCGCY	2 cut(s)	408, 2203
HaeIII	GGCC	3 cut(s)	305, 1404, 2220
HgaI	GACGC	1 cut(s)	2400
HhaI	GCGC	3 cut(s)	407, 1912, 2202
Hin1I	GRCGYC	1 cut(s)	405
Hin6I	GCGC	3 cut(s)	405, 1910, 2200
HinP1I	GCGC	3 cut(s)	405, 1910, 2200
HincII	GTYRAC	1 cut(s)	2011
HindII	GTYRAC	1 cut(s)	2011
HindIII	AAGCTT	3 cut(s)	971, 1232, 2689
HinfI	GANTC	9 cut(s)	129, 542, 1055, 1126, 1935, 2048, 2270, 2845, 2885
HpaI	GTTAAC	1 cut(s)	2011
HphI	GGTGA	8 cut(s)	317, 665, 758, 1422, 1739, 1875, 2297, 2765
Hpy166II	GTNNAC	6 cut(s)	749, 1430, 1728, 1981, 2011, 2430
Hpy8I	GTNNAC	6 cut(s)	749, 1430, 1728, 1981, 2011, 2430
HpyCH4III	ACNGT	7 cut(s)	210, 394, 512, 1331, 1631, 1732, 2615
HpyCH4IV	ACGT	2 cut(s)	2095, 2284
HpyF3I	CTNAG	7 cut(s)	75, 269, 935, 959, 1617, 1697, 1961
HpySE526I	ACGT	2 cut(s)	2095, 2284
Hsp92I	GRCGYC	1 cut(s)	405
HspAI	GCGC	3 cut(s)	405, 1910, 2200
KasI	GGCGCC	1 cut(s)	404
Kpn2I	TCCGGA	1 cut(s)	2758
KpnI	GGTACC	2 cut(s)	146, 2781
Ksp22I	TGATCA	1 cut(s)	715
KspAI	GTTAAC	1 cut(s)	2011
LguI	GCTCTTC	2 cut(s)	525, 2209
LmnI	GCTCC	6 cut(s)	253, 1291, 1504, 1769, 1794, 2005
LweI	GCATC	9 cut(s)	282, 889, 1523, 1861, 1921, 2106, 2128, 2430, 2636
MaeI	CTAG	3 cut(s)	1037, 1751, 2064
MaeII	ACGT	2 cut(s)	2095, 2284
MaeIII	GTNAC	5 cut(s)	37, 1372, 1504, 1541, 1745
MbiI	CCGCTC	2 cut(s)	1499, 1965
MfeI	CAATTG	3 cut(s)	570, 738, 2454
MflI	RGATCY	4 cut(s)	167, 226, 1094, 1778
MhlI	GDGCHC	8 cut(s)	208, 291, 537, 862, 943, 1259, 1442, 1983
Mly113I	GGCGCC	1 cut(s)	405
MlyI	GAGTC	4 cut(s)	138, 1120, 2057, 2854
MmeI	TCCRAC	2 cut(s)	67, 1860
Mph1103I	ATGCAT	2 cut(s)	1198, 2121
MroI	TCCGGA	1 cut(s)	2758
MroXI	GAANNNNTTC	1 cut(s)	2766
MseI	TTAA	8 cut(s)	399, 645, 798, 1023, 1110, 1392, 2010, 2693
MslI	CAYNNNNRTG	4 cut(s)	1818, 1986, 2402, 2543
MspA1I	CMGCKG	1 cut(s)	2816
MunI	CAATTG	3 cut(s)	570, 738, 2454
Mva1269I	GAATGC	2 cut(s)	2534, 2582
MvaI	CCWGG	7 cut(s)	153, 1251, 1365, 1406, 1785, 1932, 2799
NarI	GGCGCC	1 cut(s)	405
NciI	CCSGG	6 cut(s)	627, 857, 1593, 1802, 2194, 2232
NcoI	CCATGG	3 cut(s)	1639, 2377, 2772
NdeI	CATATG	2 cut(s)	1956, 2638
NlaIV	GGNNCC	8 cut(s)	144, 288, 406, 1345, 1796, 2742, 2763, 2779
NmuCI	GTSAC	5 cut(s)	37, 1372, 1504, 1541, 1745
NsbI	TGCGCA	1 cut(s)	1911
NsiI	ATGCAT	2 cut(s)	1198, 2121
NspI	RCATGY	2 cut(s)	2036, 2227
PaeI	GCATGC	1 cut(s)	2036
PagI	TCATGA	1 cut(s)	412
PceI	AGGCCT	1 cut(s)	305
PciI	ACATGT	1 cut(s)	2223
PciSI	GCTCTTC	2 cut(s)	525, 2209
PctI	GAATGC	2 cut(s)	2534, 2582
PdmI	GAANNNNTTC	1 cut(s)	2766
PfeI	GAWTC	5 cut(s)	542, 1055, 1935, 2270, 2885
PflMI	CCANNNNNTGG	2 cut(s)	1872, 2750
PfoI	TCCNGGA	2 cut(s)	1363, 1783
PleI	GAGTC	4 cut(s)	137, 1120, 2056, 2853
PluTI	GGCGCC	1 cut(s)	408
PpsI	GAGTC	4 cut(s)	137, 1120, 2056, 2853
PscI	ACATGT	1 cut(s)	2223
PshBI	ATTAAT	2 cut(s)	399, 798
PsiI	TTATAA	2 cut(s)	633, 729
Psp6I	CCWGG	7 cut(s)	151, 1249, 1363, 1404, 1783, 1930, 2797
PspEI	GGTNACC	1 cut(s)	1745
PspFI	CCCAGC	2 cut(s)	248, 1241
PspGI	CCWGG	7 cut(s)	151, 1249, 1363, 1404, 1783, 1930, 2797
PspN4I	GGNNCC	8 cut(s)	144, 288, 406, 1345, 1796, 2742, 2763, 2779
PspPI	GGNCC	3 cut(s)	1725, 1901, 2741
PstI	CTGCAG	1 cut(s)	1180
PstNI	CAGNNNCTG	3 cut(s)	100, 1184, 1558
PsuI	RGATCY	4 cut(s)	167, 226, 1094, 1778
PvuII	CAGCTG	1 cut(s)	2816
RsaI	GTAC	6 cut(s)	144, 1844, 1969, 2191, 2429, 2779
RsaNI	GTAC	6 cut(s)	143, 1843, 1968, 2190, 2428, 2778
RseI	CAYNNNNRTG	4 cut(s)	1818, 1986, 2402, 2543
SapI	GCTCTTC	2 cut(s)	525, 2209
SaqAI	TTAA	8 cut(s)	399, 645, 798, 1023, 1110, 1392, 2010, 2693
Sau96I	GGNCC	3 cut(s)	1725, 1901, 2741
SchI	GAGTC	4 cut(s)	138, 1120, 2057, 2854
SduI	GDGCHC	8 cut(s)	208, 291, 537, 862, 943, 1259, 1442, 1983
SfaNI	GCATC	9 cut(s)	282, 889, 1523, 1861, 1921, 2106, 2128, 2430, 2636
SfcI	CTRYAG	2 cut(s)	1176, 1518
SfoI	GGCGCC	1 cut(s)	406
SinI	GGWCC	3 cut(s)	1725, 1901, 2741
SmiMI	CAYNNNNRTG	4 cut(s)	1818, 1986, 2402, 2543
SmlI	CTYRAG	3 cut(s)	430, 1275, 2686
SmoI	CTYRAG	3 cut(s)	430, 1275, 2686
SphI	GCATGC	1 cut(s)	2036
SseBI	AGGCCT	1 cut(s)	305
SsiI	CCGC	7 cut(s)	125, 354, 743, 790, 1497, 1798, 1965
SspDI	GGCGCC	1 cut(s)	404
SspI	AATATT	2 cut(s)	649, 796
SspMI	CTAG	3 cut(s)	1037, 1751, 2064
StuI	AGGCCT	1 cut(s)	305
StyI	CCWWGG	6 cut(s)	300, 1027, 1639, 2377, 2772, 2880
TaaI	ACNGT	7 cut(s)	210, 394, 512, 1331, 1631, 1732, 2615
TaiI	ACGT	2 cut(s)	2098, 2287
TaqI	TCGA	6 cut(s)	989, 2243, 2470, 2502, 2550, 2809
TatI	WGTACW	1 cut(s)	2427
TauI	GCSGC	1 cut(s)	1800
TfiI	GAWTC	5 cut(s)	542, 1055, 1935, 2270, 2885
Tru1I	TTAA	8 cut(s)	399, 645, 798, 1023, 1110, 1392, 2010, 2693
Tru9I	TTAA	8 cut(s)	399, 645, 798, 1023, 1110, 1392, 2010, 2693
TscAI	CASTG	9 cut(s)	691, 963, 1509, 1663, 1735, 1741, 1903, 2471, 2620
TseFI	GTSAC	5 cut(s)	37, 1372, 1504, 1541, 1745
Tsp45I	GTSAC	5 cut(s)	37, 1372, 1504, 1541, 1745
TspGWI	ACGGA	6 cut(s)	1109, 1415, 1529, 1779, 2750, 2880
TspRI	CASTG	9 cut(s)	691, 963, 1509, 1663, 1735, 1741, 1903, 2471, 2620
Van91I	CCANNNNNTGG	2 cut(s)	1872, 2750
VneI	GTGCAC	1 cut(s)	1979
VpaK11BI	GGWCC	3 cut(s)	1725, 1901, 2741
VspI	ATTAAT	2 cut(s)	399, 798
XceI	RCATGY	2 cut(s)	2036, 2227
XmnI	GAANNNNTTC	1 cut(s)	2766
XspI	CTAG	3 cut(s)	1037, 1751, 2064
Zsp2I	ATGCAT	2 cut(s)	1198, 2121

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.