Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0004303

Physical Location & Seq

Genomic Coordinates Forward (+)

28356819 .. 28357724

Gene Structure 906 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1138500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 453 bp

ATGGATCACAACAACCAAATATTCTTCCTTGGTTCATCAGCATCCTCATCAGCTTCTCCTGTTTTTTCATCGAGTCAGTCAAGTTTTCCACTTTGTCTCGGTGACAGAAATGCAGGCATGATGAAGTCAGGAAGAAAAGAAGGTGATAAGGTGAACAAGAAGCAGAAATATGCATTTCAAACAAGGAGCCAGGTTGACATACTTGACGACGGGTACCGGTGGCGAAAATACGGGCAAAAAGTTGTTAAGAACAATAAGTTTCCTAGAAGTTACTATAAGTGTACGCATCAAGGGTGCATGGTGAAGAAACAAATCCAACGCCTTTCGAAGGACGAAGGGATTGTGGTAACGACATACGATGGAATCCATACGCATCGGATTGATGAGTATTCTGCCGAAAATCTTGAGCAGATAATTAGGCATATGCAAGCATCTACTGTGCCTAATAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000122 GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0006950 GO:0007275 GO:0008150 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010015 GO:0010053 GO:0010055 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0022622 GO:0030154 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032101 GO:0032104 GO:0032107 GO:0032501 GO:0032502 GO:0033554 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043620 GO:0044212 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048364 GO:0048519 GO:0048523 GO:0048527 GO:0048528 GO:0048583 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0065007 GO:0080090 GO:0090558 GO:0090627 GO:0090696 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:1905392 GO:1990837 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

151

Amino Acids

17.15

Weight (kDa)

9.56

Isoelectric Point (pI)

53.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	69 - 125	2.4e-26	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004303)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13080
fragaria_vesca	FvH4_4g23480 FvH4_5g08610
malus_domestica	MD06G1138500.v1.1 MD13G1122100.v1.1 MD14G1154500.v1.1 MD16G1122400.v1.1
prunus_persica	Prupe.1G223200_v2.0.a1 Prupe.5G148700_v2.0.a1 Prupe.5G148700_v2.0.a1
pyrus_communis	pycom06g12970 pycom13g10630 pycom14g12860 pycom16g10490
rosa_chinensis	RchiOBHm_Chr4g0429851 RchiOBHm_Chr7g0189781
rosa_laevigata	RLG00000004591 RLG00000007038
rosa_multiflora	Rmu_sc0002225.1_g000001
rosa_roxburghii	Rroxscaffold_3G00265170 Rroxscaffold_5G00371610
rosa_rugosa	Rorug04G0238600 Rorug06G0499800
rosa_samantha	Rh4AG295000 Rh4BG301400 Rh4CG316600 Rh4DG298300 Rh7AG104900 Rh7CG109500 Rh7DG107900
rosa_wichuraiana	Rw4G025530 Rw7G009080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	213
AccB1I	GGYRCC	1 cut(s)	213
AclWI	GGATC	1 cut(s)	12
AfaI	GTAC	2 cut(s)	215, 283
AfiI	CCNNNNNNNGG	1 cut(s)	328
AgeI	ACCGGT	1 cut(s)	216
AgsI	TTSAA	1 cut(s)	179
AjnI	CCWGG	1 cut(s)	189
AluBI	AGCT	1 cut(s)	53
AluI	AGCT	1 cut(s)	53
Alw26I	GTCTC	1 cut(s)	101
AlwI	GGATC	1 cut(s)	12
AsiGI	ACCGGT	1 cut(s)	216
Asp718I	GGTACC	1 cut(s)	213
AsuHPI	GGTGA	4 cut(s)	113, 155, 163, 313
AsuII	TTCGAA	1 cut(s)	326
BanI	GGYRCC	1 cut(s)	213
BccI	CCATC	1 cut(s)	353
BciT130I	CCWGG	1 cut(s)	191
BcoDI	GTCTC	1 cut(s)	101
BfaI	CTAG	1 cut(s)	264
Bme1390I	CCNGG	1 cut(s)	191
BmiI	GGNNCC	2 cut(s)	188, 215
BmrFI	CCNGG	1 cut(s)	191
BmsI	GCATC	4 cut(s)	50, 295, 382, 440
Bpu14I	TTCGAA	1 cut(s)	326
BpuEI	CTTGAG	1 cut(s)	425
BsaJI	CCNNGG	1 cut(s)	28
BsaWI	WCCGGW	1 cut(s)	216
Bsc4I	CCNNNNNNNGG	1 cut(s)	328
Bse118I	RCCGGY	1 cut(s)	216
BseBI	CCWGG	1 cut(s)	191
BseDI	CCNNGG	1 cut(s)	28
BseGI	GGATG	1 cut(s)	41
BseLI	CCNNNNNNNGG	1 cut(s)	328
BshNI	GGYRCC	1 cut(s)	213
BshTI	ACCGGT	1 cut(s)	216
BsiSI	CCGG	1 cut(s)	217
BslI	CCNNNNNNNGG	1 cut(s)	328
BsmAI	GTCTC	1 cut(s)	101
Bsp119I	TTCGAA	1 cut(s)	326
Bsp143I	GATC	1 cut(s)	4
BspLI	GGNNCC	2 cut(s)	188, 215
BspPI	GGATC	1 cut(s)	12
BspT104I	TTCGAA	1 cut(s)	326
BspT107I	GGYRCC	1 cut(s)	213
BsrFI	RCCGGY	1 cut(s)	216
BssAI	RCCGGY	1 cut(s)	216
BssECI	CCNNGG	1 cut(s)	28
BssMI	GATC	1 cut(s)	4
BssT1I	CCWWGG	1 cut(s)	28
Bst2UI	CCWGG	1 cut(s)	191
Bst4CI	ACNGT	1 cut(s)	439
BstBI	TTCGAA	1 cut(s)	326
BstC8I	GCNNGC	2 cut(s)	115, 429
BstENI	CCTNNNNNAGG	1 cut(s)	326
BstF5I	GGATG	1 cut(s)	41
BstKTI	GATC	1 cut(s)	7
BstMAI	GTCTC	1 cut(s)	101
BstMBI	GATC	1 cut(s)	4
BstNI	CCWGG	1 cut(s)	191
BstSCI	CCNGG	1 cut(s)	189
BtsCI	GGATG	1 cut(s)	41
Cac8I	GCNNGC	2 cut(s)	115, 429
Cfr10I	RCCGGY	1 cut(s)	216
Csp6I	GTAC	2 cut(s)	214, 282
CspAI	ACCGGT	1 cut(s)	216
CviAII	CATG	2 cut(s)	118, 298
CviJI	RGCY	2 cut(s)	53, 189
CviKI_1	RGCY	2 cut(s)	53, 189
CviQI	GTAC	2 cut(s)	214, 282
DpnI	GATC	1 cut(s)	6
DpnII	GATC	1 cut(s)	4
Eco130I	CCWWGG	1 cut(s)	28
EcoNI	CCTNNNNNAGG	1 cut(s)	326
EcoRII	CCWGG	1 cut(s)	189
EcoT14I	CCWWGG	1 cut(s)	28
EcoT22I	ATGCAT	1 cut(s)	175
ErhI	CCWWGG	1 cut(s)	28
FaeI	CATG	2 cut(s)	121, 301
FaiI	YATR	9 cut(s)	119, 171, 200, 276, 299, 355, 369, 423, 425
FatI	CATG	2 cut(s)	117, 297
FauNDI	CATATG	1 cut(s)	423
FokI	GGATG	1 cut(s)	28
FspBI	CTAG	1 cut(s)	264
HapII	CCGG	1 cut(s)	217
Hin1II	CATG	2 cut(s)	121, 301
HincII	GTYRAC	1 cut(s)	196
HindII	GTYRAC	1 cut(s)	196
HinfI	GANTC	2 cut(s)	73, 363
HpaII	CCGG	1 cut(s)	217
HphI	GGTGA	4 cut(s)	113, 155, 163, 313
Hpy166II	GTNNAC	3 cut(s)	154, 196, 282
Hpy188I	TCNGA	1 cut(s)	378
Hpy188III	TCNNGA	2 cut(s)	129, 404
Hpy8I	GTNNAC	3 cut(s)	154, 196, 282
Hpy99I	CGWCG	1 cut(s)	212
HpyAV	CCTTC	3 cut(s)	134, 322, 329
HpyCH4III	ACNGT	1 cut(s)	439
HpyCH4V	TGCA	4 cut(s)	113, 173, 297, 427
Hsp92II	CATG	2 cut(s)	121, 301
KpnI	GGTACC	1 cut(s)	217
Kzo9I	GATC	1 cut(s)	4
LmnI	GCTCC	1 cut(s)	186
LpnPI	CCDG	6 cut(s)	72, 99, 114, 176, 203, 230
LweI	GCATC	4 cut(s)	50, 295, 382, 440
MaeI	CTAG	1 cut(s)	264
MaeIII	GTNAC	3 cut(s)	101, 269, 346
MalI	GATC	1 cut(s)	6
MboI	GATC	1 cut(s)	4
MboII	GAAGA	3 cut(s)	16, 144, 316
MluCI	AATT	2 cut(s)	414, 448
MlyI	GAGTC	1 cut(s)	82
MmeI	TCCRAC	1 cut(s)	340
MnlI	CCTC	1 cut(s)	55
Mph1103I	ATGCAT	1 cut(s)	175
MseI	TTAA	2 cut(s)	246, 451
MspI	CCGG	1 cut(s)	217
MspR9I	CCNGG	1 cut(s)	191
MvaI	CCWGG	1 cut(s)	191
NdeI	CATATG	1 cut(s)	423
NdeII	GATC	1 cut(s)	4
NlaIII	CATG	2 cut(s)	121, 301
NlaIV	GGNNCC	2 cut(s)	188, 215
NmuCI	GTSAC	1 cut(s)	101
NsiI	ATGCAT	1 cut(s)	175
NspV	TTCGAA	1 cut(s)	326
PfeI	GAWTC	1 cut(s)	363
PinAI	ACCGGT	1 cut(s)	216
PleI	GAGTC	1 cut(s)	81
PpsI	GAGTC	1 cut(s)	81
Psp6I	CCWGG	1 cut(s)	189
PspGI	CCWGG	1 cut(s)	189
PspN4I	GGNNCC	2 cut(s)	188, 215
RsaI	GTAC	2 cut(s)	215, 283
RsaNI	GTAC	2 cut(s)	214, 282
SaqAI	TTAA	2 cut(s)	246, 451
Sau3AI	GATC	1 cut(s)	4
SchI	GAGTC	1 cut(s)	82
ScrFI	CCNGG	1 cut(s)	191
SetI	ASST	4 cut(s)	55, 145, 153, 195
SfaNI	GCATC	4 cut(s)	50, 295, 382, 440
SfuI	TTCGAA	1 cut(s)	326
SmlI	CTYRAG	1 cut(s)	404
SmoI	CTYRAG	1 cut(s)	404
Sse9I	AATT	2 cut(s)	414, 448
SspI	AATATT	1 cut(s)	21
SspMI	CTAG	1 cut(s)	264
StyD4I	CCNGG	1 cut(s)	189
StyI	CCWWGG	1 cut(s)	28
TaaI	ACNGT	1 cut(s)	439
TaqI	TCGA	2 cut(s)	71, 326
TasI	AATT	2 cut(s)	414, 448
TfiI	GAWTC	1 cut(s)	363
Tru1I	TTAA	2 cut(s)	246, 451
Tru9I	TTAA	2 cut(s)	246, 451
TseFI	GTSAC	1 cut(s)	101
Tsp45I	GTSAC	1 cut(s)	101
TspDTI	ATGAA	3 cut(s)	24, 57, 137
XagI	CCTNNNNNAGG	1 cut(s)	326
XspI	CTAG	1 cut(s)	264
Zsp2I	ATGCAT	1 cut(s)	175

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.