Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0004303

Physical Location & Seq

Genomic Coordinates Forward (+)

24914608 .. 24915904

Gene Structure 1297 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1154500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 447 bp

ATGTACACTATACTTTTAGTCATAAATTTTTCCTCTCAGATGGATCAAAATAACCAAATATTCTTCCTCGGTTCATCAACAACCTCACCAGCTTTTCTTACTTGTTCAGCGAACCAGTCAAGCTTTCCTCATTTTCGCAGTGACAGAAGTGAAGGCCTGATGAAGTCAGGAGGAAAAGAAGGTGACAAAGAGAGCAAGAAGCACAAATATGCATTTCAAACAAGGAGCCAGGTTGATATACTTGATGACGGGTACCGGTGGCGAAAATACGGGCAAAAATTTGTTAAAAACAATAAATTTCCTAGAAGTTACTATAGGTGTACACATCGAGGATGCATCGTCAAGAAACAAGTCCAACGCCATTCGAAAGACGAGGGGATTGTGGTAACGACATACGATGGAATCCATACGCATCGGGTTGATGAGAATTCTGCTGGAAATTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000122 GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0006950 GO:0007275 GO:0008150 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010015 GO:0010053 GO:0010055 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0022622 GO:0030154 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032101 GO:0032104 GO:0032107 GO:0032501 GO:0032502 GO:0033554 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043620 GO:0044212 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048364 GO:0048519 GO:0048523 GO:0048527 GO:0048528 GO:0048583 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0065007 GO:0080090 GO:0090558 GO:0090627 GO:0090696 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:1905392 GO:1990837 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

149

Amino Acids

17.09

Weight (kDa)

9.59

Isoelectric Point (pI)

44.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	82 - 138	3.1e-26	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004303)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13080
fragaria_vesca	FvH4_4g23480 FvH4_5g08610
malus_domestica	MD06G1138500.v1.1 MD13G1122100.v1.1 MD14G1154500.v1.1 MD16G1122400.v1.1
prunus_persica	Prupe.1G223200_v2.0.a1 Prupe.5G148700_v2.0.a1 Prupe.5G148700_v2.0.a1
pyrus_communis	pycom06g12970 pycom13g10630 pycom14g12860 pycom16g10490
rosa_chinensis	RchiOBHm_Chr4g0429851 RchiOBHm_Chr7g0189781
rosa_laevigata	RLG00000004591 RLG00000007038
rosa_multiflora	Rmu_sc0002225.1_g000001
rosa_roxburghii	Rroxscaffold_3G00265170 Rroxscaffold_5G00371610
rosa_rugosa	Rorug04G0238600 Rorug06G0499800
rosa_samantha	Rh4AG295000 Rh4BG301400 Rh4CG316600 Rh4DG298300 Rh7AG104900 Rh7CG109500 Rh7DG107900
rosa_wichuraiana	Rw4G025530 Rw7G009080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	252
AccB1I	GGYRCC	1 cut(s)	252
AclWI	GGATC	1 cut(s)	51
AcsI	RAATTY	5 cut(s)	25, 278, 296, 427, 439
AfaI	GTAC	3 cut(s)	5, 254, 322
AgeI	ACCGGT	1 cut(s)	255
AgsI	TTSAA	1 cut(s)	218
AjnI	CCWGG	1 cut(s)	228
AluBI	AGCT	2 cut(s)	92, 123
AluI	AGCT	2 cut(s)	92, 123
AlwI	GGATC	1 cut(s)	51
AoxI	GGCC	1 cut(s)	154
ApoI	RAATTY	5 cut(s)	25, 278, 296, 427, 439
AsiGI	ACCGGT	1 cut(s)	255
Asp718I	GGTACC	1 cut(s)	252
AsuHPI	GGTGA	2 cut(s)	78, 194
AsuII	TTCGAA	1 cut(s)	365
BanI	GGYRCC	1 cut(s)	252
BccI	CCATC	2 cut(s)	34, 392
BciT130I	CCWGG	1 cut(s)	230
BfaI	CTAG	1 cut(s)	303
BfmI	CTRYAG	1 cut(s)	313
Bme1390I	CCNGG	1 cut(s)	230
BmiI	GGNNCC	2 cut(s)	227, 254
BmrFI	CCNGG	1 cut(s)	230
BmsI	GCATC	3 cut(s)	323, 345, 421
Bpu14I	TTCGAA	1 cut(s)	365
BsaJI	CCNNGG	1 cut(s)	67
BsaWI	WCCGGW	1 cut(s)	255
Bse118I	RCCGGY	1 cut(s)	255
Bse1I	ACTGG	1 cut(s)	115
BseBI	CCWGG	1 cut(s)	230
BseDI	CCNNGG	1 cut(s)	67
BseGI	GGATG	1 cut(s)	338
BseMII	CTCAG	1 cut(s)	50
BseNI	ACTGG	1 cut(s)	115
BshFI	GGCC	1 cut(s)	156
BshNI	GGYRCC	1 cut(s)	252
BshTI	ACCGGT	1 cut(s)	255
BsiSI	CCGG	1 cut(s)	256
BsnI	GGCC	1 cut(s)	156
Bsp119I	TTCGAA	1 cut(s)	365
Bsp1407I	TGTACA	2 cut(s)	3, 320
Bsp143I	GATC	1 cut(s)	43
BspANI	GGCC	1 cut(s)	156
BspCNI	CTCAG	1 cut(s)	49
BspLI	GGNNCC	2 cut(s)	227, 254
BspPI	GGATC	1 cut(s)	51
BspT104I	TTCGAA	1 cut(s)	365
BspT107I	GGYRCC	1 cut(s)	252
BsrFI	RCCGGY	1 cut(s)	255
BsrGI	TGTACA	2 cut(s)	3, 320
BsrI	ACTGG	1 cut(s)	115
BssAI	RCCGGY	1 cut(s)	255
BssECI	CCNNGG	1 cut(s)	67
BssMI	GATC	1 cut(s)	43
Bst2UI	CCWGG	1 cut(s)	230
BstAUI	TGTACA	2 cut(s)	3, 320
BstBI	TTCGAA	1 cut(s)	365
BstDEI	CTNAG	1 cut(s)	36
BstF5I	GGATG	1 cut(s)	338
BstKTI	GATC	1 cut(s)	46
BstMBI	GATC	1 cut(s)	43
BstNI	CCWGG	1 cut(s)	230
BstSCI	CCNGG	1 cut(s)	228
BstSFI	CTRYAG	1 cut(s)	313
BsuRI	GGCC	1 cut(s)	156
BtsCI	GGATG	1 cut(s)	338
BtsI	GCAGTG	1 cut(s)	145
BtsIMutI	CAGTG	1 cut(s)	145
Cfr10I	RCCGGY	1 cut(s)	255
Csp6I	GTAC	3 cut(s)	4, 253, 321
CspAI	ACCGGT	1 cut(s)	255
CviJI	RGCY	4 cut(s)	92, 123, 156, 228
CviKI_1	RGCY	4 cut(s)	92, 123, 156, 228
CviQI	GTAC	3 cut(s)	4, 253, 321
DdeI	CTNAG	1 cut(s)	36
DpnI	GATC	1 cut(s)	45
DpnII	GATC	1 cut(s)	43
Eco147I	AGGCCT	1 cut(s)	156
EcoRI	GAATTC	1 cut(s)	427
EcoRII	CCWGG	1 cut(s)	228
EcoT22I	ATGCAT	2 cut(s)	214, 338
FaiI	YATR	7 cut(s)	11, 23, 210, 239, 315, 394, 408
FokI	GGATG	1 cut(s)	345
FspBI	CTAG	1 cut(s)	303
HaeIII	GGCC	1 cut(s)	156
HapII	CCGG	1 cut(s)	256
HindIII	AAGCTT	1 cut(s)	121
HinfI	GANTC	1 cut(s)	402
HpaII	CCGG	1 cut(s)	256
HphI	GGTGA	2 cut(s)	78, 194
Hpy166II	GTNNAC	3 cut(s)	6, 321, 323
Hpy188I	TCNGA	1 cut(s)	39
Hpy188III	TCNNGA	2 cut(s)	168, 343
Hpy8I	GTNNAC	3 cut(s)	6, 321, 323
HpyAV	CCTTC	2 cut(s)	146, 173
HpyCH4V	TGCA	2 cut(s)	212, 336
HpyF3I	CTNAG	1 cut(s)	36
KpnI	GGTACC	1 cut(s)	256
Kzo9I	GATC	1 cut(s)	43
LmnI	GCTCC	1 cut(s)	225
LpnPI	CCDG	8 cut(s)	102, 128, 153, 170, 215, 242, 269, 420
LweI	GCATC	3 cut(s)	323, 345, 421
MaeI	CTAG	1 cut(s)	303
MaeIII	GTNAC	4 cut(s)	140, 182, 308, 385
MalI	GATC	1 cut(s)	45
MboI	GATC	1 cut(s)	43
MboII	GAAGA	1 cut(s)	55
MluCI	AATT	5 cut(s)	25, 278, 296, 427, 439
MmeI	TCCRAC	1 cut(s)	379
MnlI	CCTC	7 cut(s)	43, 77, 94, 138, 164, 323, 367
Mph1103I	ATGCAT	2 cut(s)	214, 338
MseI	TTAA	1 cut(s)	285
MslI	CAYNNNNRTG	1 cut(s)	207
MspI	CCGG	1 cut(s)	256
MspR9I	CCNGG	1 cut(s)	230
MvaI	CCWGG	1 cut(s)	230
NdeII	GATC	1 cut(s)	43
NlaIV	GGNNCC	2 cut(s)	227, 254
NmuCI	GTSAC	2 cut(s)	140, 182
NsiI	ATGCAT	2 cut(s)	214, 338
NspV	TTCGAA	1 cut(s)	365
PceI	AGGCCT	1 cut(s)	156
PfeI	GAWTC	1 cut(s)	402
PinAI	ACCGGT	1 cut(s)	255
Psp6I	CCWGG	1 cut(s)	228
PspGI	CCWGG	1 cut(s)	228
PspN4I	GGNNCC	2 cut(s)	227, 254
RsaI	GTAC	3 cut(s)	5, 254, 322
RsaNI	GTAC	3 cut(s)	4, 253, 321
RseI	CAYNNNNRTG	1 cut(s)	207
SaqAI	TTAA	1 cut(s)	285
Sau3AI	GATC	1 cut(s)	43
ScrFI	CCNGG	1 cut(s)	230
SetI	ASST	6 cut(s)	86, 94, 125, 184, 234, 320
SfaNI	GCATC	3 cut(s)	323, 345, 421
SfcI	CTRYAG	1 cut(s)	313
SfuI	TTCGAA	1 cut(s)	365
SmiMI	CAYNNNNRTG	1 cut(s)	207
Sse9I	AATT	5 cut(s)	25, 278, 296, 427, 439
SseBI	AGGCCT	1 cut(s)	156
SspI	AATATT	1 cut(s)	60
SspMI	CTAG	1 cut(s)	303
StuI	AGGCCT	1 cut(s)	156
StyD4I	CCNGG	1 cut(s)	228
TaqI	TCGA	2 cut(s)	328, 365
TasI	AATT	5 cut(s)	25, 278, 296, 427, 439
TatI	WGTACW	2 cut(s)	3, 320
TfiI	GAWTC	1 cut(s)	402
Tru1I	TTAA	1 cut(s)	285
Tru9I	TTAA	1 cut(s)	285
TscAI	CASTG	1 cut(s)	145
TseFI	GTSAC	2 cut(s)	140, 182
Tsp45I	GTSAC	2 cut(s)	140, 182
TspDTI	ATGAA	2 cut(s)	63, 176
TspRI	CASTG	1 cut(s)	145
XapI	RAATTY	5 cut(s)	25, 278, 296, 427, 439
XspI	CTAG	1 cut(s)	303
Zsp2I	ATGCAT	2 cut(s)	214, 338

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.