Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0004303

Physical Location & Seq

Genomic Coordinates Reverse (-)

8993050 .. 8995368

Gene Structure 2319 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1122100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 573 bp

ATGGAAAATTACCCAACATTCTTTTCTTCATCCACGGCGCCTGCTCCTTCTCCCCTGTCGTTGAACATGGGGAACCCAGCTCATCACGTTTACAATAGTAATGATCCTCGCCAATTCCAAAATAGGAAATCATCGAATGGGTTCTTAGGGCTGATGTCAGAGATGGAGGCTTCAAACAATATGATTAAGAACAATTTTAGTTCTCAGGGAAAAAGCTTTGGAGGATCTGAAAGTGGTGAAGCAACTGTGAGATTAGGGATGAAAAAAGGAGATCAGAAAAAAATAAGAAAACCCAGATATGCTTTTCAAACAAGAAGTCAAGTTGATATACTTGATGATGGATATAGATGGAGGAAGTATGGTCAAAAAGCAGTGAAGAACAACAAATTTCCAAGAAGCTACTACAGGTGCACACATCACGGTTGCAATGTGAAAAAGCAAGTTCAAAGGTTAACAAAAGATGAAGGAGTCGTTGTGACAACTTATGAAGGCATGCATTCTCATCCCATCGAGAAGTCTACTGATAACTTTGAGCATATTTTGAGCCAGATGAAAATCTACACTCCATTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000122 GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0006950 GO:0007275 GO:0008150 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010015 GO:0010053 GO:0010055 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0022622 GO:0030154 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032101 GO:0032104 GO:0032107 GO:0032501 GO:0032502 GO:0033554 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043620 GO:0044212 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048364 GO:0048519 GO:0048523 GO:0048527 GO:0048528 GO:0048583 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0065007 GO:0080090 GO:0090558 GO:0090627 GO:0090696 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:1905392 GO:1990837 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

191

Amino Acids

21.71

Weight (kDa)

9.74

Isoelectric Point (pI)

42.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	112 - 169	6.1e-27	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0004303)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13080
fragaria_vesca	FvH4_4g23480 FvH4_5g08610
malus_domestica	MD06G1138500.v1.1 MD13G1122100.v1.1 MD14G1154500.v1.1 MD16G1122400.v1.1
prunus_persica	Prupe.1G223200_v2.0.a1 Prupe.5G148700_v2.0.a1 Prupe.5G148700_v2.0.a1
pyrus_communis	pycom06g12970 pycom13g10630 pycom14g12860 pycom16g10490
rosa_chinensis	RchiOBHm_Chr4g0429851 RchiOBHm_Chr7g0189781
rosa_laevigata	RLG00000004591 RLG00000007038
rosa_multiflora	Rmu_sc0002225.1_g000001
rosa_roxburghii	Rroxscaffold_3G00265170 Rroxscaffold_5G00371610
rosa_rugosa	Rorug04G0238600 Rorug06G0499800
rosa_samantha	Rh4AG295000 Rh4BG301400 Rh4CG316600 Rh4DG298300 Rh7AG104900 Rh7CG109500 Rh7DG107900
rosa_wichuraiana	Rw4G025530 Rw7G009080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	37
AccI	GTMKAC	1 cut(s)	518
AclWI	GGATC	2 cut(s)	98, 232
AcsI	RAATTY	1 cut(s)	386
AcyI	GRCGYC	1 cut(s)	38
AgsI	TTSAA	4 cut(s)	64, 174, 308, 446
AjuI	GAANNNNNNNTTGG	2 cut(s)	7, 39
AluBI	AGCT	3 cut(s)	80, 216, 399
AluI	AGCT	3 cut(s)	80, 216, 399
Alw21I	GWGCWC	1 cut(s)	413
Alw44I	GTGCAC	1 cut(s)	409
AlwI	GGATC	2 cut(s)	98, 232
ApaLI	GTGCAC	1 cut(s)	409
ApoI	RAATTY	1 cut(s)	386
ArsI	GACNNNNNNTTYG	2 cut(s)	301, 333
Asp700I	GAANNNNTTC	1 cut(s)	140
AspLEI	GCGC	1 cut(s)	40
AsuHPI	GGTGA	1 cut(s)	248
BaeGI	GKGCMC	1 cut(s)	413
BanI	GGYRCC	1 cut(s)	37
Bbv12I	GWGCWC	1 cut(s)	413
BccI	CCATC	4 cut(s)	157, 332, 342, 515
BceAI	ACGGC	1 cut(s)	51
BfmI	CTRYAG	1 cut(s)	403
BfoI	RGCGCY	1 cut(s)	41
BmiI	GGNNCC	2 cut(s)	39, 74
BsaBI	GATNNNNATC	1 cut(s)	554
BsaHI	GRCGYC	1 cut(s)	38
BsaJI	CCNNGG	1 cut(s)	33
BsaXI	ACNNNNNCTCC	2 cut(s)	459, 489
Bse3DI	GCAATG	1 cut(s)	433
Bse8I	GATNNNNATC	1 cut(s)	554
BseDI	CCNNGG	1 cut(s)	33
BseGI	GGATG	3 cut(s)	29, 264, 502
BseJI	GATNNNNATC	1 cut(s)	554
BseMI	GCAATG	1 cut(s)	433
BseMII	CTCAG	1 cut(s)	218
BseSI	GKGCMC	1 cut(s)	413
BseYI	CCCAGC	1 cut(s)	76
BshNI	GGYRCC	1 cut(s)	37
BsiHKAI	GWGCWC	1 cut(s)	413
BsmI	GAATGC	1 cut(s)	496
Bsp1286I	GDGCHC	1 cut(s)	413
Bsp143I	GATC	3 cut(s)	103, 224, 271
BspCNI	CTCAG	1 cut(s)	217
BspLI	GGNNCC	2 cut(s)	39, 74
BspPI	GGATC	2 cut(s)	98, 232
BspT107I	GGYRCC	1 cut(s)	37
BsrDI	GCAATG	1 cut(s)	433
BssECI	CCNNGG	1 cut(s)	33
BssMI	GATC	3 cut(s)	103, 224, 271
BssNI	GRCGYC	1 cut(s)	38
Bst4CI	ACNGT	2 cut(s)	247, 422
BstACI	GRCGYC	1 cut(s)	38
BstC8I	GCNNGC	2 cut(s)	42, 494
BstDEI	CTNAG	2 cut(s)	145, 204
BstDSI	CCRYGG	1 cut(s)	33
BstF5I	GGATG	3 cut(s)	29, 264, 502
BstH2I	RGCGCY	1 cut(s)	41
BstHHI	GCGC	1 cut(s)	40
BstKTI	GATC	3 cut(s)	106, 227, 274
BstMBI	GATC	3 cut(s)	103, 224, 271
BstNSI	RCATGY	1 cut(s)	496
BstSFI	CTRYAG	1 cut(s)	403
BstSLI	GKGCMC	1 cut(s)	413
BstX2I	RGATCY	1 cut(s)	224
BstYI	RGATCY	1 cut(s)	224
BtgI	CCRYGG	1 cut(s)	33
BtsCI	GGATG	3 cut(s)	29, 264, 502
BtsI	GCAGTG	1 cut(s)	378
BtsIMutI	CAGTG	1 cut(s)	378
Cac8I	GCNNGC	2 cut(s)	42, 494
CfoI	GCGC	1 cut(s)	40
CviAII	CATG	2 cut(s)	67, 493
CviJI	RGCY	6 cut(s)	80, 151, 170, 216, 399, 546
CviKI_1	RGCY	6 cut(s)	80, 151, 170, 216, 399, 546
DdeI	CTNAG	2 cut(s)	145, 204
DinI	GGCGCC	1 cut(s)	39
DpnI	GATC	3 cut(s)	105, 226, 273
DpnII	GATC	3 cut(s)	103, 224, 271
EcoT22I	ATGCAT	1 cut(s)	498
EgeI	GGCGCC	1 cut(s)	39
EheI	GGCGCC	1 cut(s)	39
FaeI	CATG	2 cut(s)	70, 496
FaiI	YATR	9 cut(s)	68, 182, 300, 329, 345, 360, 486, 494, 537
FatI	CATG	2 cut(s)	66, 492
FblI	GTMKAC	1 cut(s)	518
FokI	GGATG	3 cut(s)	16, 271, 489
GlaI	GCGC	1 cut(s)	39
GsaI	CCCAGC	1 cut(s)	80
HaeII	RGCGCY	1 cut(s)	41
HhaI	GCGC	1 cut(s)	40
Hin1I	GRCGYC	1 cut(s)	38
Hin1II	CATG	2 cut(s)	70, 496
Hin6I	GCGC	1 cut(s)	38
HinP1I	GCGC	1 cut(s)	38
HincII	GTYRAC	1 cut(s)	453
HindII	GTYRAC	1 cut(s)	453
HindIII	AAGCTT	1 cut(s)	214
HinfI	GANTC	1 cut(s)	468
HpaI	GTTAAC	1 cut(s)	453
HphI	GGTGA	1 cut(s)	248
Hpy166II	GTNNAC	4 cut(s)	91, 411, 453, 519
Hpy188I	TCNGA	3 cut(s)	160, 229, 276
Hpy188III	TCNNGA	1 cut(s)	511
Hpy8I	GTNNAC	4 cut(s)	91, 411, 453, 519
HpyAV	CCTTC	3 cut(s)	57, 458, 482
HpyCH4III	ACNGT	2 cut(s)	247, 422
HpyCH4IV	ACGT	1 cut(s)	87
HpyCH4V	TGCA	3 cut(s)	411, 426, 496
HpyF3I	CTNAG	2 cut(s)	145, 204
HpySE526I	ACGT	1 cut(s)	87
Hsp92I	GRCGYC	1 cut(s)	38
Hsp92II	CATG	2 cut(s)	70, 496
HspAI	GCGC	1 cut(s)	38
KasI	GGCGCC	1 cut(s)	37
KspAI	GTTAAC	1 cut(s)	453
Kzo9I	GATC	3 cut(s)	103, 224, 271
LmnI	GCTCC	1 cut(s)	49
LpnPI	CCDG	7 cut(s)	54, 68, 90, 191, 307, 391, 560
MaeII	ACGT	1 cut(s)	87
MaeIII	GTNAC	1 cut(s)	475
MalI	GATC	3 cut(s)	105, 226, 273
MboI	GATC	3 cut(s)	103, 224, 271
MboII	GAAGA	2 cut(s)	18, 388
MflI	RGATCY	1 cut(s)	224
MhlI	GDGCHC	1 cut(s)	413
MluCI	AATT	4 cut(s)	7, 113, 193, 386
Mly113I	GGCGCC	1 cut(s)	38
MlyI	GAGTC	1 cut(s)	477
MnlI	CCTC	4 cut(s)	117, 160, 215, 345
Mph1103I	ATGCAT	1 cut(s)	498
MroXI	GAANNNNTTC	1 cut(s)	140
MseI	TTAA	3 cut(s)	186, 452, 571
Mva1269I	GAATGC	1 cut(s)	496
NarI	GGCGCC	1 cut(s)	38
NdeII	GATC	3 cut(s)	103, 224, 271
NlaIII	CATG	2 cut(s)	70, 496
NlaIV	GGNNCC	2 cut(s)	39, 74
NmuCI	GTSAC	1 cut(s)	475
NsiI	ATGCAT	1 cut(s)	498
NspI	RCATGY	1 cut(s)	496
PaeI	GCATGC	1 cut(s)	496
PctI	GAATGC	1 cut(s)	496
PdmI	GAANNNNTTC	1 cut(s)	140
PleI	GAGTC	1 cut(s)	476
PluTI	GGCGCC	1 cut(s)	41
PpsI	GAGTC	1 cut(s)	476
PspFI	CCCAGC	1 cut(s)	76
PspN4I	GGNNCC	2 cut(s)	39, 74
PsuI	RGATCY	1 cut(s)	224
SaqAI	TTAA	3 cut(s)	186, 452, 571
Sau3AI	GATC	3 cut(s)	103, 224, 271
SchI	GAGTC	1 cut(s)	477
SduI	GDGCHC	1 cut(s)	413
SetI	ASST	6 cut(s)	82, 90, 218, 401, 410, 452
SfcI	CTRYAG	1 cut(s)	403
SfoI	GGCGCC	1 cut(s)	39
SphI	GCATGC	1 cut(s)	496
Sse9I	AATT	4 cut(s)	7, 113, 193, 386
SspDI	GGCGCC	1 cut(s)	37
TaaI	ACNGT	2 cut(s)	247, 422
TaiI	ACGT	1 cut(s)	90
TaqI	TCGA	2 cut(s)	134, 510
TasI	AATT	4 cut(s)	7, 113, 193, 386
Tru1I	TTAA	3 cut(s)	186, 452, 571
Tru9I	TTAA	3 cut(s)	186, 452, 571
TscAI	CASTG	1 cut(s)	378
TseFI	GTSAC	1 cut(s)	475
Tsp45I	GTSAC	1 cut(s)	475
TspDTI	ATGAA	5 cut(s)	18, 275, 477, 501, 566
TspRI	CASTG	1 cut(s)	378
VneI	GTGCAC	1 cut(s)	409
XapI	RAATTY	1 cut(s)	386
XceI	RCATGY	1 cut(s)	496
XmiI	GTMKAC	1 cut(s)	518
XmnI	GAANNNNTTC	1 cut(s)	140
Zsp2I	ATGCAT	1 cut(s)	498

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.