Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0004303

Physical Location & Seq

Genomic Coordinates Forward (+)

60859012 .. 60873694

Gene Structure 14683 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG295000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 837 bp

ATGATGGAAACCTACCCAACATTTTACTCTTCTTCATCAACGACACCACCTGCTGCTGCTTCCTCTATGTCATTGAACATGGTGAACAGTCATCCTCATCATGCTTATAGTAACGATCAGCACCAAGCTAGCAACAATAAGTCAAATGGGTTCTTGGGGCTGATGTCAGAGATGGAGGTTTCGAACAGTATCAACAGCATGAGTAGTATTTCCCAGAGCATGAAAAGCTTTGGGGAGGGTGAAAGTAATACAGCAGTAAGGGCAGGGATGAAGAAGGGAGAGAAGAAGATCAGGAAGCCGAGATATGCGTTTCAGACCAGGAGCCAAGTCGATATACTTGACGATGGATACAGGTGGAGAAAGTATGGCCAAAAAGCAGTCAAGAACAACAAATTCCCAAGAAGCTACTATCGGTGCACGCATCAGGGCTGCAATGTGAAAAAGCAGGTTCAGAGACTAACCAAGGATGAAGGAGTTGTGGTGACTACATATGAAGGCATGCACTCTCATCCCATTGAGAAACGTCACGAAGCACAGCGTCTTGGTCGTATTCTGAGGGCTAAGGTTATCAAGGCAAATGCTGGTCTTACCCCTGAGCATTATCAGTTTTTCCTGTACCAGCTTGTTCGTGGTTTAAAGTATATTCATACAGGTTTATACACAACTAACCGCCTTCTATTTATTGCTCTAAATTCATCATTCTATCTCCATTATTTCAATGTATATCAAAAACGTAAGCAAGCGAAGATTATGCGAGCTATTGTTGTATACTTGCACAAGAATTCATTTGATGCAAAAATCAATGAGATGTCGGTGAAGACCGAACTAGTTAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000122 GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0006950 GO:0007275 GO:0008150 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010015 GO:0010053 GO:0010055 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0022622 GO:0030154 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032101 GO:0032104 GO:0032107 GO:0032501 GO:0032502 GO:0033554 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043620 GO:0044212 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048364 GO:0048519 GO:0048523 GO:0048527 GO:0048528 GO:0048583 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0065007 GO:0080090 GO:0090558 GO:0090627 GO:0090696 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:1905392 GO:1990837 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

278

Amino Acids

31.96

Weight (kDa)

9.94

Isoelectric Point (pI)

43.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	114 - 171	6.9e-27	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004303)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13080
fragaria_vesca	FvH4_4g23480 FvH4_5g08610
malus_domestica	MD06G1138500.v1.1 MD13G1122100.v1.1 MD14G1154500.v1.1 MD16G1122400.v1.1
prunus_persica	Prupe.1G223200_v2.0.a1 Prupe.5G148700_v2.0.a1 Prupe.5G148700_v2.0.a1
pyrus_communis	pycom06g12970 pycom13g10630 pycom14g12860 pycom16g10490
rosa_chinensis	RchiOBHm_Chr4g0429851 RchiOBHm_Chr7g0189781
rosa_laevigata	RLG00000004591 RLG00000007038
rosa_multiflora	Rmu_sc0002225.1_g000001
rosa_roxburghii	Rroxscaffold_3G00265170 Rroxscaffold_5G00371610
rosa_rugosa	Rorug04G0238600 Rorug06G0499800
rosa_samantha	Rh4AG295000 Rh4BG301400 Rh4CG316600 Rh4DG298300 Rh7AG104900 Rh7CG109500 Rh7DG107900
rosa_wichuraiana	Rw4G025530 Rw7G009080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	58
Acc36I	ACCTGC	2 cut(s)	58, 436
AccI	GTMKAC	1 cut(s)	768
AciI	CCGC	1 cut(s)	670
AcoI	YGGCCR	1 cut(s)	367
AcsI	RAATTY	3 cut(s)	392, 691, 781
AfaI	GTAC	1 cut(s)	617
AgsI	TTSAA	2 cut(s)	76, 718
AhlI	ACTAGT	1 cut(s)	826
AjnI	CCWGG	1 cut(s)	317
AluBI	AGCT	5 cut(s)	128, 228, 405, 622, 758
AluI	AGCT	5 cut(s)	128, 228, 405, 622, 758
Alw21I	GWGCWC	1 cut(s)	419
Alw26I	GTCTC	1 cut(s)	448
Alw44I	GTGCAC	1 cut(s)	415
AoxI	GGCC	1 cut(s)	367
ApaLI	GTGCAC	1 cut(s)	415
ApeKI	GCWGC	3 cut(s)	53, 56, 429
ApoI	RAATTY	3 cut(s)	392, 691, 781
AsuHPI	GGTGA	4 cut(s)	94, 251, 493, 826
AsuII	TTCGAA	1 cut(s)	182
AsuNHI	GCTAGC	1 cut(s)	128
BaeGI	GKGCMC	1 cut(s)	419
BalI	TGGCCA	1 cut(s)	369
BbsI	GAAGAC	1 cut(s)	824
Bbv12I	GWGCWC	1 cut(s)	419
BbvI	GCAGC	3 cut(s)	40, 43, 416
BccI	CCATC	2 cut(s)	166, 338
BcgI	CGANNNNNNTGC	2 cut(s)	733, 767
BciT130I	CCWGG	1 cut(s)	319
BciVI	GTATCC	1 cut(s)	341
BcoDI	GTCTC	1 cut(s)	448
BcuI	ACTAGT	1 cut(s)	826
BfaI	CTAG	2 cut(s)	129, 827
BfuAI	ACCTGC	2 cut(s)	58, 436
BfuI	GTATCC	1 cut(s)	341
BisI	GCNGC	3 cut(s)	54, 57, 430
BlsI	GCNGC	3 cut(s)	55, 58, 431
Bme1390I	CCNGG	1 cut(s)	319
BmiI	GGNNCC	1 cut(s)	323
BmrFI	CCNGG	1 cut(s)	319
BmsI	GCATC	2 cut(s)	430, 781
BmtI	GCTAGC	1 cut(s)	132
BpiI	GAAGAC	1 cut(s)	824
Bpu10I	CCTNAGC	2 cut(s)	561, 594
Bpu14I	TTCGAA	1 cut(s)	182
BsaJI	CCNNGG	1 cut(s)	462
BsaXI	ACNNNNNCTCC	2 cut(s)	465, 495
Bse3DI	GCAATG	1 cut(s)	439
BseBI	CCWGG	1 cut(s)	319
BseDI	CCNNGG	1 cut(s)	462
BseGI	GGATG	4 cut(s)	91, 273, 472, 508
BseMI	GCAATG	1 cut(s)	439
BseMII	CTCAG	2 cut(s)	545, 585
BseSI	GKGCMC	1 cut(s)	419
BseXI	GCAGC	3 cut(s)	40, 43, 416
BshFI	GGCC	1 cut(s)	369
BsiHKAI	GWGCWC	1 cut(s)	419
BsmAI	GTCTC	1 cut(s)	448
BsnI	GGCC	1 cut(s)	369
Bsp119I	TTCGAA	1 cut(s)	182
Bsp1286I	GDGCHC	1 cut(s)	419
Bsp143I	GATC	2 cut(s)	115, 288
BspACI	CCGC	1 cut(s)	670
BspANI	GGCC	1 cut(s)	369
BspCNI	CTCAG	2 cut(s)	546, 586
BspLI	GGNNCC	1 cut(s)	323
BspMI	ACCTGC	2 cut(s)	58, 436
BspOI	GCTAGC	1 cut(s)	132
BspT104I	TTCGAA	1 cut(s)	182
BsrDI	GCAATG	1 cut(s)	439
BssECI	CCNNGG	1 cut(s)	462
BssMI	GATC	2 cut(s)	115, 288
BssNAI	GTATAC	1 cut(s)	769
BssT1I	CCWWGG	1 cut(s)	462
Bst1107I	GTATAC	1 cut(s)	769
Bst2UI	CCWGG	1 cut(s)	319
Bst4CI	ACNGT	2 cut(s)	89, 188
Bst6I	CTCTTC	1 cut(s)	34
BstBI	TTCGAA	1 cut(s)	182
BstC8I	GCNNGC	5 cut(s)	130, 419, 500, 741, 756
BstDEI	CTNAG	3 cut(s)	554, 561, 594
BstF5I	GGATG	4 cut(s)	91, 273, 472, 508
BstKTI	GATC	2 cut(s)	118, 291
BstMAI	GTCTC	1 cut(s)	448
BstMBI	GATC	2 cut(s)	115, 288
BstMWI	GCNNNNNNNGC	2 cut(s)	225, 260
BstNI	CCWGG	1 cut(s)	319
BstNSI	RCATGY	1 cut(s)	502
BstSCI	CCNGG	1 cut(s)	317
BstSLI	GKGCMC	1 cut(s)	419
BstV1I	GCAGC	3 cut(s)	40, 43, 416
BstV2I	GAAGAC	1 cut(s)	824
BstZ17I	GTATAC	1 cut(s)	769
BsuI	GTATCC	1 cut(s)	341
BsuRI	GGCC	1 cut(s)	369
BtsCI	GGATG	4 cut(s)	91, 273, 472, 508
BveI	ACCTGC	2 cut(s)	58, 436
Cac8I	GCNNGC	5 cut(s)	130, 419, 500, 741, 756
CseI	GACGC	1 cut(s)	527
Csp6I	GTAC	1 cut(s)	616
CviAII	CATG	5 cut(s)	79, 101, 199, 220, 499
CviQI	GTAC	1 cut(s)	616
DdeI	CTNAG	3 cut(s)	554, 561, 594
DpnI	GATC	2 cut(s)	117, 290
DpnII	GATC	2 cut(s)	115, 288
DraI	TTTAAA	1 cut(s)	636
EaeI	YGGCCR	1 cut(s)	367
Eam1104I	CTCTTC	1 cut(s)	34
EarI	CTCTTC	1 cut(s)	34
Eco130I	CCWWGG	1 cut(s)	462
EcoRI	GAATTC	1 cut(s)	781
EcoRII	CCWGG	1 cut(s)	317
EcoT14I	CCWWGG	1 cut(s)	462
ErhI	CCWWGG	1 cut(s)	462
FaeI	CATG	5 cut(s)	82, 104, 202, 223, 502
FatI	CATG	5 cut(s)	78, 100, 198, 219, 498
FauNDI	CATATG	1 cut(s)	490
FblI	GTMKAC	1 cut(s)	768
Fnu4HI	GCNGC	3 cut(s)	54, 57, 430
FokI	GGATG	4 cut(s)	78, 280, 479, 495
Fsp4HI	GCNGC	3 cut(s)	54, 57, 430
FspBI	CTAG	2 cut(s)	129, 827
GluI	GCNGC	3 cut(s)	54, 57, 430
HaeIII	GGCC	1 cut(s)	369
HgaI	GACGC	1 cut(s)	527
Hin1II	CATG	5 cut(s)	82, 104, 202, 223, 502
HincII	GTYRAC	1 cut(s)	832
HindII	GTYRAC	1 cut(s)	832
HindIII	AAGCTT	1 cut(s)	226
HpaI	GTTAAC	1 cut(s)	832
HphI	GGTGA	4 cut(s)	94, 251, 493, 826
Hpy166II	GTNNAC	4 cut(s)	85, 417, 769, 832
Hpy188I	TCNGA	4 cut(s)	169, 315, 453, 555
Hpy188III	TCNNGA	3 cut(s)	292, 382, 527
Hpy8I	GTNNAC	4 cut(s)	85, 417, 769, 832
HpyAV	CCTTC	4 cut(s)	268, 464, 488, 683
HpyCH4III	ACNGT	2 cut(s)	89, 188
HpyCH4IV	ACGT	2 cut(s)	523, 733
HpyCH4V	TGCA	5 cut(s)	417, 432, 502, 775, 794
HpyF10VI	GCNNNNNNNGC	2 cut(s)	225, 260
HpyF3I	CTNAG	3 cut(s)	554, 561, 594
HpySE526I	ACGT	2 cut(s)	523, 733
Hsp92II	CATG	5 cut(s)	82, 104, 202, 223, 502
KspAI	GTTAAC	1 cut(s)	832
Kzo9I	GATC	2 cut(s)	115, 288
LmnI	GCTCC	1 cut(s)	321
Lsp1109I	GCAGC	3 cut(s)	40, 43, 416
LweI	GCATC	2 cut(s)	430, 781
MaeI	CTAG	2 cut(s)	129, 827
MaeII	ACGT	2 cut(s)	523, 733
MaeIII	GTNAC	3 cut(s)	110, 481, 524
MalI	GATC	2 cut(s)	117, 290
MboI	GATC	2 cut(s)	115, 288
MboII	GAAGA	7 cut(s)	21, 24, 283, 295, 298, 757, 829
MhlI	GDGCHC	1 cut(s)	419
MlsI	TGGCCA	1 cut(s)	369
MluCI	AATT	3 cut(s)	392, 691, 781
MluNI	TGGCCA	1 cut(s)	369
MnlI	CCTC	5 cut(s)	73, 105, 169, 229, 549
Mox20I	TGGCCA	1 cut(s)	369
MscI	TGGCCA	1 cut(s)	369
MseI	TTAA	2 cut(s)	635, 831
Msp20I	TGGCCA	1 cut(s)	369
MspR9I	CCNGG	1 cut(s)	319
MvaI	CCWGG	1 cut(s)	319
MwoI	GCNNNNNNNGC	2 cut(s)	225, 260
NdeI	CATATG	1 cut(s)	490
NdeII	GATC	2 cut(s)	115, 288
NheI	GCTAGC	1 cut(s)	128
NlaIII	CATG	5 cut(s)	82, 104, 202, 223, 502
NlaIV	GGNNCC	1 cut(s)	323
NmeAIII	GCCGAG	1 cut(s)	324
NmuCI	GTSAC	2 cut(s)	481, 524
NspI	RCATGY	1 cut(s)	502
NspV	TTCGAA	1 cut(s)	182
PaeI	GCATGC	1 cut(s)	502
PaqCI	CACCTGC	1 cut(s)	58
PkrI	GCNGC	3 cut(s)	55, 58, 431
Psp6I	CCWGG	1 cut(s)	317
PspGI	CCWGG	1 cut(s)	317
PspN4I	GGNNCC	1 cut(s)	323
RsaI	GTAC	1 cut(s)	617
RsaNI	GTAC	1 cut(s)	616
SaqAI	TTAA	2 cut(s)	635, 831
SatI	GCNGC	3 cut(s)	54, 57, 430
Sau3AI	GATC	2 cut(s)	115, 288
ScrFI	CCNGG	1 cut(s)	319
SduI	GDGCHC	1 cut(s)	419
SfaNI	GCATC	2 cut(s)	430, 781
SfuI	TTCGAA	1 cut(s)	182
SpeI	ACTAGT	1 cut(s)	826
SphI	GCATGC	1 cut(s)	502
Sse9I	AATT	3 cut(s)	392, 691, 781
SsiI	CCGC	1 cut(s)	670
SspMI	CTAG	2 cut(s)	129, 827
StyD4I	CCNGG	1 cut(s)	317
StyI	CCWWGG	1 cut(s)	462
TaaI	ACNGT	2 cut(s)	89, 188
TaiI	ACGT	2 cut(s)	526, 736
TaqI	TCGA	2 cut(s)	182, 330
TaqII	GACCGA	1 cut(s)	836
TasI	AATT	3 cut(s)	392, 691, 781
Tru1I	TTAA	2 cut(s)	635, 831
Tru9I	TTAA	2 cut(s)	635, 831
TseFI	GTSAC	2 cut(s)	481, 524
TseI	GCWGC	3 cut(s)	53, 56, 429
Tsp45I	GTSAC	2 cut(s)	481, 524
TspDTI	ATGAA	8 cut(s)	24, 236, 284, 483, 507, 635, 684, 774
VneI	GTGCAC	1 cut(s)	415
XapI	RAATTY	3 cut(s)	392, 691, 781
XceI	RCATGY	1 cut(s)	502
XcmI	CCANNNNNNNNNTGG	1 cut(s)	626
XmiI	GTMKAC	1 cut(s)	768
XspI	CTAG	2 cut(s)	129, 827

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.