MD06G1173700.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0002934
Physical Location & Seq
Forward (+)
31350632 .. 31391023
40392 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1173700.v1.1.491

Sequence Viewer

Length: 9063 bp
ATGGTGACTTCCAGAGACGTCCAGGAGATCGTCTCCAAGCTCTCCTCCGATAAAGCCAAGACTCGAGAAGAAGGGATAAAGTTTTTGAATACATGGTTAGAAGGAGAAAGATCGATTGGTTTCTGTAAATATCTTGGAAAGAAGACTGCAGAGCTTAAACCAGATGAAATTCCTCACTCTGAGACCTGGCCTTTCTTTACTAATTTGCTGATTCAGTGTGCTTCATTGGAGATATCCTCCTCAAGCAAGCGGCGATTGCCCAAGTTGATATGTGCAAAGAGTCTAAGGATAGTTGTGCAACACGCAGAGGATGATAAGTTTTCAGGCAAGCGGTTGCCTTTGTTAGCTGTGGTTAAAAATCTTTTTAATCATATATGTGAGGTCTTACATAATGATAATGTCCCAAGCTTTCAATTGGAATATGGGATTATTCTCCGGCATCTATTAACTGTCAGAGACTATCGATTTCAGATGAAGAGGCGCACCTATTGCAGCCTAATACAGAAGTACATGGATAAGATAGACGAAAGCTTGAGCCAATATAATACCAAGGAGGAGGTCTTTCGCTGCATTCTTACTCTCCATTCACTTTTGGAGAATCCCCCTGGAGATTTCCTACCAAATCTTAGGGAAGAGATTGTTGAGTGGTTTGTCAGAATTTTTTCTCACATTAGGGATGAAGGAAAGATTTCACGCAAACTTATGGACTGCATTAATACATTTTTGTTGAAAGATGGTCCAAATTTGGGTTCTGAGTCCTTGGAGATCCATAATGCTGTGCAGCAATTTGTGTTCCGGTGTTGGCTGACAACCCATGATCGAGCCCTTAAGGATGCACTTATTTTTTATGCAAGGTTACAGCTGAATATGACTAGAGGTGCTGCTGATGGTAGTTTTTTGGTACATCAACTTCTTGACATAGTTTACAAGGAGCTGGATCAAAGCTACGTATCCAATACTAATGTGCCTAGGGCTGATGGAGCTAAAGATGACAAGTTTGGAATTTTAAGTAGCTCACATTGTGGTTTGGTTGAGCTTGCAGCTGCTGTACTTTATCGGGCTTGTCATAACATGACTAAAGCACCGTCAACTGAAAAGCGGGTCAAGAGAGAGCATGCTGCTGCACAATTGAGAGAGGCTCTCATGAAAGGAAAATGGTTATGGAATGCTTCATTTTGTTGTCTGATTCGTAAGTACCACACACACATTAGTAAGGATCTTTTCATGTACTGGTTCGAGGGCATATGTGCAAGCTTTCAAAGAATTTTGAATGATGCAAACATGGTACATTCTTATGATGGTCTGCTATGGACACTGAGCTTGCAGGAACTTTCTTCTGTGCTGTTGCTTCCAGATTCAAAGTTGAAGAAACTGTCAAGCTCGTCTCCCTTGAATGAGTTTGATTGTGGTTGGCAAGTAGTGTGGAGTTGCCTGATGCAGGGGTTGCCATTGTTTAGTAATGTAGCTGCAGTTGTAGATGCTGCCCTGGTGCTCCTTGGAAACTTAATTTCAAATGATCTTGTGCATAAATGTGTAGTACCTCAGGAGGTTTGGGACCTTAGGTTATTCAAACAAATGCCATCAGTGTCTGTCTTGTACTTTATTTCATGTTACTTCTCGAGGAAAGGTACTCAAGGTGATGTTCGTGACATTTTACATCTGAGAAAAAACCTCCTGAGAGAACTTCTGGGTCAACTTCAGTTGATGGAGTCTTCCATGCTTAATGAGCACTTGGTGGTATTGTTACCTGCAGCTGTTTATGCTCTCTGTGCCGGTTGCACTCCTTTTGATCAATGGTGTAGAGGACTTCCTTTATCTTACTCTTTTGTCGATATTACTGAGGCTGTTGATAAGCCAGAAGATCCTGAGCACGATTGTCTGCATGGGCTGTTTGACTGCAATGTGGAAGTTCTCGCTAGTATCAATCCAGGTTCCAGCGTTGAGGTTCTTCCATCCCAAACCCATCCAAGTGTATGGCTTCCGCGACAATTAAGGGATCCACTGTTTTTTGAGATGGAAACCTTTATTCTTGAATCTCTAGTAGAGAAGGAGATGGGAAGGAGGCCTCTATCTGATGTGTTCTTTATATGTACGCTATTGTCAAACTTCATCTATGGTTCAATATTGACAAGGCAAAGAGAGGAAGCATCATTATTCCTTTCAAAATTGGGCCAATACCTGTTAGAATGGCTGGATTGTGCAGTTAAGGCCACCCAAGGAAACCACAATGATATTCTGCCTCTTGCTTGCTTGGGCTCAGATCTTGTCTCTAATGGAACAAGCTCCATTGTTGCTTCCTTAAAGAGTTTTCTTTACTCCCCTATATTCATGAGGTGGAGAGATCAAAATCATGCGGATGTTGACCTTTATGGTGCTATCATGCAAATGATGGAGAGGCTTTTAAAATCATTGGCTAGTGTGTATGAAGAATACTCTAGCAGTGCAAGTAGTCATAAGTTTGATGTGACTCAGCAAGACTTCTCTTCTGATAGAAGTAAAATTAGGATCATGGACATGGAGTTGGATGTGAATGATGATTCCAAAGATGTGGATGTATTAACTATTGGTGATAAAATTGGTGGTGGTGTATCCTCTTCCATAGAGAAATGGAAGCTGGGCATGATTTTGCTTATTTCAAATTTTTTCTCAGTTTCACACGTTACTTGGGATGTCCTATATGATCTCTTGTCAAAAGAAACTGATCAAATGGTTCGTGAAAATATTCTGCTCTTTCTTTGTCAACATCCATACTGGTCATCTCATGAAAATCTTACAGACATGATCAATTTAATGAATGAAATGGTTAAGATGCAAGCCAGACTTAAGCTTGATAGTGCTATGGTGTTATCTGCTATCCGTGGTTTGTTGGGCACCTTATCATCCTTGGACTCTATCAGGAAGGATAAATTTGTCATCGTCTCTTTGGGGCAAAGAGGATATGAGCAGAGCTTGATGCATCTTGGAGATTTGGTGAACAAAGTTGCTGAATGTGATCTTGACTGGTTTTGTCGTGCTAAGCTAATTGATTGCATATGCAATTTTATTTTACTTAGTCCTCAAATTGGTCAGACACTGATTGAAAGGTTGCTGTTGATGTTCCGAGATCCTGATTATCGAGTTCGGTTCTCCCTGGCTAGACAAATTGGTGTTCTTTTCCAAACATGGGATGGACATGAAGAATTATTCCATGATATTTGTTCCAACTTTGGTGTTGTGTTGGTGTTTTTCTCTCAAGAAAAATGTGTTGCTGCAAGTGAGGTCATAGCGGCCGGTCCACAGCCTCGTCCCACAATGGAGACTGTCATTATTACTCTTATGCATCTTGCTTTGCATAGTGAGAAAATAGAGTTCGAGGCTGTATTCATGATATGTGTGGTTTCTGCTATTGATCCTTGTCAGAGGGAATTGGTCTTTGCAGTACTTGATAATCTCTCCAGCCAGCTGCATTATACAAATAGGTTCAAGTACCTGGAGGAGATTATAGGATCAATTCTCTTTTGTTGGATTGCTTGTGGTGTAAGCCTTGCTGCACTTGTGGAGATTAGGCAATTGTTTGTATCAGATTCGGAACCTAGTTATTTCATGCAGTATTGCTGTCATTGGCTTCTCCCGGCTCTACTTCTGCATGGGGACAGCTCCAATCTCAGCTGGGTTGCCAAGATTGCTGGTCAGCCTCTGGAAGTTCTGGTGAAAAATCACTTTGTGCAGATTTTTTCAGTTTGTATGGGATTGCATTGTAGTAAGAAATCTGGGTGGGAAAAGGGAGCAGATGTGCTTCAGAATTCAGTTTTGCGTCTTGCTCAGATATCTGAGAATGAGCGGGACAAACTAATTAAGAAACATATGGCATCTATTGTCAGCCACATTCTGTCTCTTGGCTCGTCTGCATCAAACCCTGCAGTTCCATTCTTTTCTAGGAACACGATTGCAAGTGCAATTCAAACAGTTGTTGACGGATTTTTGGAGAAGGAGGATTATGCTACAAGTATCTGTGTTGTTGACAAGATTAACATTTTTCGACCAGATAGGGTTTTCAAGTTTATAGTGGAACTGCACTATAAGATTGCAGCAGCAACTCACCATAGGCATGCATGTCACCGGCTTGTTGGAGTTGAGGTGCTTATAGATATTCTTGGGTATAGAGCTGCTGTTGCTAGCACGTCCAATTATCTTTTCAATTTGGTTGGGCAATTCATTGGCTGCCAGGCTTTACTAGATCAATGTTGTCGTATCATTTCTGCACTGCTGAAAACTTTTAAAAGTAAACCATCAAAAGAAATTATCTGTGTTCTGGGTGAGCAACTTCAGTTTTTAGTGTCAAAGTTGGTGACATGTTGCATTCCCTCTGAAGCTAAGGGAGAAGAATCTGGCAGATCATCCCAAGTCTTATCTTTGCTGCTCCAGCTTACTGTGGATTCTGATCCATCTCTTTATGATTATATCAGAGAATTGGAACCCTTCCCTGAAATAGATATCTTTGATGGGATTAGAAAGTTCCATCAGGAATTATGCCAAGCTTACTCTTCAAGAGATCATTTGTTGAAGTTTGCTGAGAGATCTTGTTACCTTCCGCCGAGATTACTTCTGTGGAGTCTCCAAGCACTACACAAGAAGTTTCTTTTGGGTGAGACATTTCAAAGGAAAAAGAAAGCAGTAGAACTTGTGAACGATACATATTGGCATCATGACCATGAAATTACCAATGCTGTTTGGTCACTTGTCCGCACGTGTGGCTTGGATGATACAAATACCGTTGGAGTTTTGCTCTCTGATTTTATATCTAGGGTTGGAATTGGGGACCCACATTGTGTTGTTTTTCATCTTCCAGGAAACTCTAGTAACATCCATGTCTGCCGACCTATTAATCATGACTCTTCTATAGAAGTCAAATTTCAGATGGATACCGGCTTATCTGAAGAACTTTTGGTTATGCTTTTAAAACTTCTGAAGAAATACTTGATGGATGATTCCGTCAAAATAGTAGATATGACATCTCAAGCTCTTCGGGGAATTCTTTCAACTCAAAGAGGTCAAAGTGCCCTGTTGTCTTTTGATTCTTATGAGAGGTCTCTTATTGAGGTTCACTCTAAGGGTGTCAACATTGAGCTGGTGGAAAAGCTCCTTTTGGATCTGGATATTAAGTTTAAGGCTGAAGCAATTCCATTAGAGAACTCTACTGTATGGGCAACACAGGGTAAAACTTTTGATACATGGATTTGTCCACTTGTCTTCTCACTGATTGGTTATTGCAATGACGTAATACTGAGATTATGTCAGGATATTGTGTTAGCAAAAGCTGAAGCTGCGGAGCTTCTTTTACCAAGCCTTGTTGTCAATCTTGCTGGAAGGAAGGATATAGATGTTGACCTCTTCAAATTAATCTCTTTGCAGGTGCAGGAATCTATATTTACAGACTCTAATAAGTTAATCAAATCAATTCAAATTTGGTTGAATGCTCTTAATGAACTTCGGTTATGTCATGTGATACAAAAATCTTCATCGTTCCCATCAAGGGCGGAAATATCTAAGAGCGCTAAACCTGCTAGTTGTAGTTCTAAATCCCGTTCCACCCCCGGAAAGGCCAAAGATTCAGCTCCTACATTAAGTGCAGCGGCAATGTCGACGTCTTTATGGGACAAGGTTTATTGGCTTTCTATTGATTATCTTGTCGTTGCCAAGTCAGCAGTCATTTGTGGCTCATACTTTACTGCTGTGATGTACGTGGAGCATTGGTGTGAAGAGCATTTCAACAGCCTCACACTGGGGAGCCCTGATTTCTCTCACATTGAAGTGTTGCCACATCATATCGAAATACTTGTCGCTGCGGTCACTCAGATCAATGAACCTGACAGCTTATATGGGATCATCCAGTCACACAAGTTGAGCTCTCAAATCATTACCTTCGAGCATGAGGGAAACTGGAGCAAGGCCCTTGAGTACTATGACTTGCAAGTACGTTCGGCTGCCTTGGTACCGGTGGATTTTGGTTCCAGAAATTTGTCAATGGAAGAGACTCAACCAACAGATTGTTTATCCAATCCCATATTGGAAAATGCGATGAGGCAGAGGAAACCATATAAAGGTTTGATTAGGTCATTGCAGCAAACTGGTTGTATGCATGTTCTGGATTTGTATTGCCAAGGATTGACAACTCGGAAGGGACAGTTTGATCATGATCTGGAGTTTACTGAATTGCAGTATGAGGCTGCTTGGCGTACTGCAAACTGGGATTTCTCCTTACTTTATGTTGGTGACAATTCTATTTCATCAAGTTTGCACATCAAAAGTGATAATTTCAATGAAAATTTGCATAGTTGTTTGAGGGCACTGAAAGAGGGAGATTACAATGAATTCCATGGAAAGCTGAGATATTCAAAGCAGGAGCTTGTGTGGTCTGTTTCTCGTGCAAGTGAAGAAAGTACTGAGCACATATATTCAGCAATAATTAAACTTCAGATCCTTTATCATCTTGGCATGGCTTGGGATTTACGTTGGACATCATCTGATTATGATGAGGGTATAAGTTTTCATCCAAAGGTGGAAGAAATGAATTCTGAACCTATAATCCCTACCATGGATCAGTTGTCTTGGCTGAACATGGATTGGAGTTCCATTTTGGAACGGACACAGTTACACATGAATTTACTGGAGCCTTTTATAGCGTTCAGGCGAGTGCTACTTCAAATCTTAAGCTGTAGGGAGTGTACGGAGCAACATCTTCTGCAGTCCACTTCTACCCTCCGTAAGGGTTCTAGATTTTCTCAAGCGGCTGCAGCCTTGCATGAGTTTAAGTTGCTGTGTGTCGAATCAGGAGAGCAAGATTCATCTCTATACTGGCTTGGAAGGCTTGAAGAAGCAAAGCTTCTGCGTGGCCAAGGTCAGCATGAGATGGCAATCAGCCTTGCAAAATATGTCTCGCAAAATTTCGTGTCCAATGAAGAGTCTTCAGATGTTCATCGCTTGGTTGGGAAGTGGCTGGCAGAGACTAGATCTAGCAACTCAAGAACTATTTTAGAGAAGTATTTGAAGCCTGCTGTTTCGCTTGCTGAGAATCAGAAGTATACAAAAGAGATGTCCAGAGGTAGACAAAGCCAAACACATTTTCATCTTGCACATTATGCAGATGCTCTTTTCAGGAGTTATGAAGAAAGACTTACCTCTAATGAATGGCAAGCAGCAATGCGTTTAAGGAAACACAAGACAATGGAACTGGAAGCACTTATTAAACGGCTAAGATCTTCTACAAAGGGAGAGAAGAGTGACTATTCGGTCAAAATTCAAGAGTTGCAAAAGCAACTTGCAATGGACAAGGAGGAGGCTGAAAAACTCCAGGAAGACAGGGACAATTTCCTCAGCCTAGCATTGGAGGGATATAACCGTTGTTTGGTTGTTGGTGACAAATATGATGTGCGAGTGGTTTTTCGGTTAATTTCCCTGTGGTTCTCTCTCTCTTCAAGGAAAAATGTCATAGATAGCATGCTTGCTGCAATCAGTGAGGTTCAGTCTTACAAATTCATCCCTTTAGTGTACCAAATTGCTTCAAGAATGGGTAGCTTAAAGGATTGTCCAGGACCTCAAAATTTTCAGTTTGCCTTGGTTTCTCTTCTGAAGAAAATGGCCATTGACCATCCATACCATACTATCTTTCAGCTTCTAGCATTGGCAAATGGTGACCGTATCAAGGACAAGCAGCGCAGTAGGAACTCATTTGTGGTAGATATGGATAAAAAGCTTGCAGCAGAGAATCTCTTACAAGAGTTAACATCGTATCATGGAGGCATTATAAATCAAATGAAGCAAATGGTGGAGATCTATATCAAACTTGCCGAACTAGAAACGAAGAGAGAGGATACCAATAGAAAGGTAATGCTACCGAGAGAATTGCGTAATCTTCAACCGCTGGAACTTGTGCCTGTTGTGACGGCTACCTTTCCCATTGACCGTAGTTGTCAATATCATGAAGGCTCTTTCCCATATTTCAAAGGATTAGGAGATTCAGTTGTAGTAATGAACGGTATAAATGCTCCAAAGGTGGTTGAATGCCTGGGTTCTGATGGTTGCCGATACAAACAGCTGGCAAAATCTGGAAATGATGATCTAAGACAAGATGCTGTAATGGAACAATTTTTTGGTTTAGTCAACACCTTTTTACAGAACCACCGGGATACATGGAAAAGAAGATTAGGAGTGCGTACATACAAGGTAGTTCCATTCACTCCAAGTGCTGGTGTTCTTGAATGGGTTAATGGCACTCTTCCTCTTGGTGACTATCTTATAGGAAGCACGAGAAATGGAGGGGCCCATGGTCGATATGGAGTGGGGGACTGGTCATTTCTCAAATGCCGAGAACACATGGCAAATGGAAAGGACAAGCGCAAGGCATTCAAGGAAGTTTGTACGAAGTTCAGGCCTGTCATGCATCATTTTTTCTTGGAGAGGTTCTTGCAACCAGCTGACTGGTTTGAGAAGAGACTTTCGTACACGCGAAGTGTTGCCACTAGTTCAATGGTTGGTTATATCGTTGGCCTTGGAGATCGACATTCTATGAATATCTTAATTGATCAAACCACTGCAGAAGTTGTTCATATAGATCTTGGCGTTGCCTTTGAACAAGGCTTGATGCTCAAAACACCAGAACGGGTTCCATTCCGACTTACAAGGGACATCATCGATGGCATGGGTGTGACTGGAGTGGAAGGGGTTTTCAGAAGATGCTGTGAAGAAACTCTTTCTGTTATGAGGACAAATAAAGAAGCACTACTGACCATCGTTGAAGTTTTTATCCATGATCCACTTTATAAGTGGGCTTTATCACCTTTGAAAGCTTTGCAGCGTCAGAAGGAAACGGATGACGACTTGAACTTAAGCTTTGAAGGCTCGCAAGATGAATATGAAGGTAACAAGGATGCTGCACGTGCACTGATGCGCGTAAAACAAAAACTCGATGGATATGAAGACGGGGAAATGCGAAGCATACATGGACAGGTTCAACAACTTATACAAGATGCGATCGATCCCGAGCGTCTATGTCAACTGTTTCCTGGATGGGGAGCATGGTTATGA

Protein Analysis

3021

Amino Acids

343.59

Weight (kDa)

6.28

Isoelectric Point (pI)

41.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAN PF11640 8 - 161 8.2e-06 Telomere-length maintenance and DNA damage repair
TPR_ATM PF25360 1185 - 1382 8.1e-101 ATM-like, TPR repeats
FAT PF02259 2064 - 2477 1.5e-10 FAT domain
PI3_PI4_kinase PF00454 2695 - 2936 9.1e-55 Phosphatidylinositol 3- and 4-kinase
FATC PF02260 2991 - 3020 1.3e-11 FATC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 7784, 8799
AarI CACCTGC 1 cut(s) 5380
AasI GACNNNNNNGTC 1 cut(s) 7268
AatII GACGTC 2 cut(s) 21, 5627
Acc36I ACCTGC 3 cut(s) 1756, 5380, 5548
Acc65I GGTACC 1 cut(s) 5971
AccB1I GGYRCC 2 cut(s) 2870, 5971
AccB7I CCANNNNNTGG 1 cut(s) 8225
AccBSI CCGCTC 1 cut(s) 3820
AccI GTMKAC 3 cut(s) 5621, 7061, 7084
AccII CGCG 3 cut(s) 1984, 8485, 8928
AcoI YGGCCR 3 cut(s) 3267, 6871, 7617
AcvI CACGTG 2 cut(s) 4728, 8915
AcyI GRCGYC 2 cut(s) 18, 5624
AdeI CACNNNGTG 4 cut(s) 1022, 1735, 3703, 4808
AfeI AGCGCT 1 cut(s) 5533
AflII CTTAAG 4 cut(s) 827, 2822, 6688, 8863
AflIII ACRYGT 3 cut(s) 2656, 4331, 4727
AgeI ACCGGT 1 cut(s) 5974
AhlI ACTAGT 1 cut(s) 8498
AjiI CACGTC 1 cut(s) 4161
AloI GAACNNNNNNTCC 2 cut(s) 6549, 6581
Alw21I GWGCWC 6 cut(s) 1494, 1731, 1872, 5888, 6429, 8920
Alw44I GTGCAC 1 cut(s) 8916
AlwNI CAGNNNCTG 4 cut(s) 1046, 1589, 3073, 3676
Ama87I CYCGRG 3 cut(s) 63, 1618, 9017
Aor51HI AGCGCT 1 cut(s) 5533
ApaI GGGCCC 1 cut(s) 8302
ApaLI GTGCAC 1 cut(s) 8916
ArsI GACNNNNNNTTYG 4 cut(s) 5802, 5834, 7058, 7090
AseI ATTAAT 3 cut(s) 714, 4863, 5378
AsiGI ACCGGT 1 cut(s) 5974
Asp700I GAANNNNTTC 7 cut(s) 661, 688, 2721, 5014, 5157, 8580, 8640
Asp718I GGTACC 1 cut(s) 5971
AspA2I CCTAGG 1 cut(s) 968
AspLEI GCGC 5 cut(s) 483, 5534, 7695, 8376, 8928
AspS9I GGNCC 9 cut(s) 737, 1555, 2170, 3272, 4798, 5929, 7571, 8298, 8299
AsuC2I CCSGG 3 cut(s) 3611, 5574, 8162
AsuNHI GCTAGC 1 cut(s) 4154
AvaI CYCGRG 3 cut(s) 63, 1618, 9017
AvaII GGWCC 5 cut(s) 737, 1555, 3272, 4798, 7571
AvrII CCTAGG 1 cut(s) 968
AxyI CCTNAGG 2 cut(s) 1542, 1559
BaeGI GKGCMC 5 cut(s) 2873, 5041, 6328, 8302, 8920
BalI TGGCCA 2 cut(s) 6873, 7619
BamHI GGATCC 1 cut(s) 1996
BanI GGYRCC 2 cut(s) 2870, 5971
BanII GRGCYC 5 cut(s) 826, 2258, 5771, 5888, 8302
BarI GAAGNNNNNNTAC 2 cut(s) 894, 926
BauI CACGAG 2 cut(s) 6402, 8284
BbrPI CACGTG 2 cut(s) 4728, 8915
BbsI GAAGAC 6 cut(s) 149, 1704, 5221, 6936, 7341, 8961
Bbv12I GWGCWC 6 cut(s) 1494, 1731, 1872, 5888, 6429, 8920
BbvCI CCTCAGC 1 cut(s) 7352
BceAI ACGGC 2 cut(s) 7244, 7938
BcgI CGANNNNNNTGC 2 cut(s) 7864, 7898
BciVI GTATCC 5 cut(s) 961, 2599, 4894, 7843, 8158
BclI TGATCA 5 cut(s) 1789, 2701, 2781, 6169, 8560
BcnI CCSGG 3 cut(s) 3611, 5574, 8162
BcuI ACTAGT 1 cut(s) 8498
BfmI CTRYAG 9 cut(s) 147, 1467, 1749, 3897, 4878, 6694, 6722, 6771, 8571
BfoI RGCGCY 1 cut(s) 5535
BfrI CTTAAG 4 cut(s) 827, 2822, 6688, 8863
BfuAI ACCTGC 3 cut(s) 1756, 5380, 5548
BfuI GTATCC 5 cut(s) 961, 2599, 4894, 7843, 8158
BglII AGATCT 6 cut(s) 2260, 4556, 6989, 7235, 7809, 8590
BlnI CCTAGG 1 cut(s) 968
BlpI GCTNAGC 1 cut(s) 3015
BmcAI AGTACT 3 cut(s) 3420, 5939, 6421
Bme18I GGWCC 5 cut(s) 737, 1555, 3272, 4798, 7571
BmeT110I CYCGRG 3 cut(s) 63, 1618, 9017
BmgBI CACGTC 1 cut(s) 4161
BmgT120I GGNCC 9 cut(s) 737, 1555, 2170, 3272, 4798, 5929, 7571, 8298, 8299
BmrI ACTGGG 2 cut(s) 5771, 6235
BmtI GCTAGC 1 cut(s) 4158
BmuI ACTGGG 2 cut(s) 5771, 6235
BpiI GAAGAC 6 cut(s) 149, 1704, 5221, 6936, 7341, 8961
BpmI CTGGAG 9 cut(s) 627, 3418, 3491, 4385, 5941, 6200, 6668, 7313, 8709
Bpu1102I GCTNAGC 1 cut(s) 3015
BpuEI CTTGAG 8 cut(s) 226, 553, 1617, 3216, 4980, 5954, 6747, 6985
BpuMI CCSGG 3 cut(s) 3611, 5574, 8162
Bsa29I ATCGAT 4 cut(s) 113, 463, 8670, 9012
BsaAI YACGTR 4 cut(s) 949, 4728, 5722, 8915
BsaBI GATNNNNATC 6 cut(s) 2346, 4419, 6174, 6893, 6954, 7814
BsaHI GRCGYC 2 cut(s) 18, 5624
BsaI GGTCTC 2 cut(s) 176, 5073
BsaWI WCCGGW 2 cut(s) 795, 5974
Bse118I RCCGGY 5 cut(s) 1772, 3269, 4098, 4904, 5974
Bse21I CCTNAGG 2 cut(s) 1542, 1559
Bse3DI GCAATG 6 cut(s) 1906, 5257, 5622, 6095, 7185, 7308
Bse8I GATNNNNATC 6 cut(s) 2346, 4419, 6174, 6893, 6954, 7814
BseCI ATCGAT 4 cut(s) 113, 463, 8670, 9012
BseJI GATNNNNATC 6 cut(s) 2346, 4419, 6174, 6893, 6954, 7814
BseMI GCAATG 6 cut(s) 1906, 5257, 5622, 6095, 7185, 7308
BseRI GAGGAG 5 cut(s) 34, 229, 569, 3488, 7328
BseSI GKGCMC 5 cut(s) 2873, 5041, 6328, 8302, 8920
BseX3I CGGCCG 1 cut(s) 3267
BseYI CCCAGC 2 cut(s) 2614, 3648
Bsh1236I CGCG 3 cut(s) 1984, 8485, 8928
Bsh1285I CGRYCG 2 cut(s) 3270, 9012
BshNI GGYRCC 2 cut(s) 2870, 5971
BshTI ACCGGT 1 cut(s) 5974
BshVI ATCGAT 4 cut(s) 113, 463, 8670, 9012
BsiEI CGRYCG 2 cut(s) 3270, 9012
BsiHKAI GWGCWC 6 cut(s) 1494, 1731, 1872, 5888, 6429, 8920
BsiHKCI CYCGRG 3 cut(s) 63, 1618, 9017
BsmBI CGTCTC 4 cut(s) 9, 37, 1389, 2923
BsmI GAATGC 6 cut(s) 570, 1171, 4338, 5458, 8045, 8381
Bso31I GGTCTC 2 cut(s) 176, 5073
BsoBI CYCGRG 3 cut(s) 63, 1618, 9017
Bsp120I GGGCCC 1 cut(s) 8298
Bsp1720I GCTNAGC 1 cut(s) 3015
Bsp19I CCATGG 3 cut(s) 6355, 6573, 8302
BspDI ATCGAT 4 cut(s) 113, 463, 8670, 9012
BspFNI CGCG 3 cut(s) 1984, 8485, 8928
BspHI TCATGA 8 cut(s) 1143, 2328, 2761, 3363, 4684, 4867, 6172, 7957
BspMAI CTGCAG 7 cut(s) 151, 1471, 1753, 3901, 6726, 6775, 8575
BspMI ACCTGC 3 cut(s) 1756, 5380, 5548
BspOI GCTAGC 1 cut(s) 4158
BspQI GCTCTTC 2 cut(s) 5007, 5733
BspT107I GGYRCC 2 cut(s) 2870, 5971
BspTI CTTAAG 4 cut(s) 827, 2822, 6688, 8863
BspTNI GGTCTC 2 cut(s) 176, 5073
BsrBI CCGCTC 1 cut(s) 3820
BsrDI GCAATG 6 cut(s) 1906, 5257, 5622, 6095, 7185, 7308
BsrFI RCCGGY 5 cut(s) 1772, 3269, 4098, 4904, 5974
BssAI RCCGGY 5 cut(s) 1772, 3269, 4098, 4904, 5974
BssNAI GTATAC 1 cut(s) 7062
BssNI GRCGYC 2 cut(s) 18, 5624
BssSI CACGAG 2 cut(s) 6402, 8284
Bst1107I GTATAC 1 cut(s) 7062
Bst2BI CACGAG 2 cut(s) 6402, 8284
BstACI GRCGYC 2 cut(s) 18, 5624
BstAFI CTTAAG 4 cut(s) 827, 2822, 6688, 8863
BstAPI GCANNNNNTGC 3 cut(s) 489, 1444, 7118
BstBAI YACGTR 4 cut(s) 949, 4728, 5722, 8915
BstDSI CCRYGG 4 cut(s) 2857, 6355, 6573, 8302
BstEII GGTNACC 1 cut(s) 7670
BstENI CCTNNNNNAGG 2 cut(s) 1436, 6743
BstFNI CGCG 3 cut(s) 1984, 8485, 8928
BstH2I RGCGCY 1 cut(s) 5535
BstHHI GCGC 5 cut(s) 483, 5534, 7695, 8376, 8928
BstMCI CGRYCG 2 cut(s) 3270, 9012
BstNSI RCATGY 6 cut(s) 1118, 4091, 4095, 4335, 6122, 7480
BstPI GGTNACC 1 cut(s) 7670
BstSFI CTRYAG 9 cut(s) 147, 1467, 1749, 3897, 4878, 6694, 6722, 6771, 8571
BstSLI GKGCMC 5 cut(s) 2873, 5041, 6328, 8302, 8920
BstSNI TACGTA 1 cut(s) 949
BstUI CGCG 3 cut(s) 1984, 8485, 8928
BstV2I GAAGAC 6 cut(s) 149, 1704, 5221, 6936, 7341, 8961
BstXI CCANNNNNNTGG 3 cut(s) 1974, 2548, 8457
BstZ17I GTATAC 1 cut(s) 7062
BstZI CGGCCG 1 cut(s) 3267
Bsu15I ATCGAT 4 cut(s) 113, 463, 8670, 9012
Bsu36I CCTNAGG 2 cut(s) 1542, 1559
BsuI GTATCC 5 cut(s) 961, 2599, 4894, 7843, 8158
BsuTUI ATCGAT 4 cut(s) 113, 463, 8670, 9012
BtgI CCRYGG 4 cut(s) 2857, 6355, 6573, 8302
BtgZI GCGATG 2 cut(s) 6071, 6941
BtrI CACGTC 1 cut(s) 4161
BtsI GCAGTG 3 cut(s) 2446, 4241, 8568
BveI ACCTGC 3 cut(s) 1756, 5380, 5548
CaiI CAGNNNCTG 4 cut(s) 1046, 1589, 3073, 3676
CciI TCATGA 8 cut(s) 1143, 2328, 2761, 3363, 4684, 4867, 6172, 7957
CfoI GCGC 5 cut(s) 483, 5534, 7695, 8376, 8928
Cfr10I RCCGGY 5 cut(s) 1772, 3269, 4098, 4904, 5974
Cfr13I GGNCC 9 cut(s) 737, 1555, 2170, 3272, 4798, 5929, 7571, 8298, 8299
ClaI ATCGAT 4 cut(s) 113, 463, 8670, 9012
CseI GACGC 3 cut(s) 3782, 8822, 9011
CspAI ACCGGT 1 cut(s) 5974
CspCI CAANNNNNGTGG 2 cut(s) 1403, 1438
DraI TTTAAA 3 cut(s) 2403, 4258, 4938
DraIII CACNNNGTG 4 cut(s) 1022, 1735, 3703, 4808
DrdI GACNNNNNNGTC 1 cut(s) 7268
DseDI GACNNNNNNGTC 1 cut(s) 7268
EaeI YGGCCR 3 cut(s) 3267, 6871, 7617
EagI CGGCCG 1 cut(s) 3267
EciI GGCGGA 2 cut(s) 4560, 5531
Ecl136II GAGCTC 1 cut(s) 5886
EclXI CGGCCG 1 cut(s) 3267
Eco105I TACGTA 1 cut(s) 949
Eco147I AGGCCT 2 cut(s) 2065, 8410
Eco24I GRGCYC 5 cut(s) 826, 2258, 5771, 5888, 8302
Eco31I GGTCTC 2 cut(s) 176, 5073
Eco32I GATATC 3 cut(s) 234, 3807, 4474
Eco47I GGWCC 5 cut(s) 737, 1555, 3272, 4798, 7571
Eco47III AGCGCT 1 cut(s) 5533
Eco52I CGGCCG 1 cut(s) 3267
Eco53kI GAGCTC 1 cut(s) 5886
Eco72I CACGTG 2 cut(s) 4728, 8915
Eco81I CCTNAGG 2 cut(s) 1542, 1559
Eco88I CYCGRG 3 cut(s) 63, 1618, 9017
Eco91I GGTNACC 1 cut(s) 7670
EcoICRI GAGCTC 1 cut(s) 5886
EcoNI CCTNNNNNAGG 2 cut(s) 1436, 6743
EcoO109I RGGNCCY 5 cut(s) 1555, 4798, 5929, 7571, 8298
EcoO65I GGTNACC 1 cut(s) 7670
EcoRI GAATTC 4 cut(s) 3781, 5010, 6350, 6550
EcoRV GATATC 3 cut(s) 234, 3807, 4474
EcoT22I ATGCAT 5 cut(s) 2958, 3321, 4093, 6120, 8421
EcoT38I GRGCYC 5 cut(s) 826, 2258, 5771, 5888, 8302
Esp3I CGTCTC 4 cut(s) 9, 37, 1389, 2923
FauI CCCGC 2 cut(s) 1092, 3813
FauNDI CATATG 3 cut(s) 1244, 3032, 3843
FbaI TGATCA 5 cut(s) 1789, 2701, 2781, 6169, 8560
FblI GTMKAC 3 cut(s) 5621, 7061, 7084
FriOI GRGCYC 5 cut(s) 826, 2258, 5771, 5888, 8302
GlaI GCGC 5 cut(s) 482, 5533, 7694, 8375, 8927
GsaI CCCAGC 2 cut(s) 2618, 3652
GsuI CTGGAG 9 cut(s) 627, 3418, 3491, 4385, 5941, 6200, 6668, 7313, 8709
HaeII RGCGCY 1 cut(s) 5535
HgaI GACGC 3 cut(s) 3782, 8822, 9011
HhaI GCGC 5 cut(s) 483, 5534, 7695, 8376, 8928
Hin1I GRCGYC 2 cut(s) 18, 5624
Hin6I GCGC 5 cut(s) 481, 5532, 7693, 8374, 8926
HinP1I GCGC 5 cut(s) 481, 5532, 7693, 8374, 8926
HindIII AAGCTT 9 cut(s) 406, 529, 1252, 2825, 4515, 6860, 7730, 8823, 8866
HpaI GTTAAC 1 cut(s) 7761
Hpy99I CGWCG 1 cut(s) 5626
Hsp92I GRCGYC 2 cut(s) 18, 5624
HspAI GCGC 5 cut(s) 481, 5532, 7693, 8374, 8926
KflI GGGWCCC 1 cut(s) 4798
KpnI GGTACC 1 cut(s) 5975
Ksp22I TGATCA 5 cut(s) 1789, 2701, 2781, 6169, 8560
KspAI GTTAAC 1 cut(s) 7761
LguI GCTCTTC 2 cut(s) 5007, 5733
MbiI CCGCTC 1 cut(s) 3820
MfeI CAATTG 3 cut(s) 413, 1127, 3548
MlsI TGGCCA 2 cut(s) 6873, 7619
MluNI TGGCCA 2 cut(s) 6873, 7619
MmeI TCCRAC 8 cut(s) 2502, 3225, 3482, 4087, 4735, 4768, 6473, 8674
Mox20I TGGCCA 2 cut(s) 6873, 7619
Mph1103I ATGCAT 5 cut(s) 2958, 3321, 4093, 6120, 8421
MroXI GAANNNNTTC 7 cut(s) 661, 688, 2721, 5014, 5157, 8580, 8640
MscI TGGCCA 2 cut(s) 6873, 7619
Msp20I TGGCCA 2 cut(s) 6873, 7619
MspA1I CMGCKG 9 cut(s) 862, 1043, 1754, 3442, 3648, 5612, 7900, 8074, 8453
MspCI CTTAAG 4 cut(s) 827, 2822, 6688, 8863
MunI CAATTG 3 cut(s) 413, 1127, 3548
Mva1269I GAATGC 6 cut(s) 570, 1171, 4338, 5458, 8045, 8381
MvnI CGCG 3 cut(s) 1984, 8485, 8928
NciI CCSGG 3 cut(s) 3611, 5574, 8162
NcoI CCATGG 3 cut(s) 6355, 6573, 8302
NdeI CATATG 3 cut(s) 1244, 3032, 3843
NheI GCTAGC 1 cut(s) 4154
NmeAIII GCCGAG 2 cut(s) 4599, 8369
NsiI ATGCAT 5 cut(s) 2958, 3321, 4093, 6120, 8421
NspI RCATGY 6 cut(s) 1118, 4091, 4095, 4335, 6122, 7480
PaeI GCATGC 3 cut(s) 1118, 4091, 7480
PaeR7I CTCGAG 2 cut(s) 63, 1618
PagI TCATGA 8 cut(s) 1143, 2328, 2761, 3363, 4684, 4867, 6172, 7957
PaqCI CACCTGC 1 cut(s) 5380
PceI AGGCCT 2 cut(s) 2065, 8410
PciI ACATGT 1 cut(s) 4331
PciSI GCTCTTC 2 cut(s) 5007, 5733
PctI GAATGC 6 cut(s) 570, 1171, 4338, 5458, 8045, 8381
PdmI GAANNNNTTC 7 cut(s) 661, 688, 2721, 5014, 5157, 8580, 8640
PflMI CCANNNNNTGG 1 cut(s) 8225
PfoI TCCNGGA 5 cut(s) 21, 4825, 7329, 7567, 9040
PinAI ACCGGT 1 cut(s) 5974
Ple19I CGATCG 1 cut(s) 9012
PmaCI CACGTG 2 cut(s) 4728, 8915
PmlI CACGTG 2 cut(s) 4728, 8915
Ppu21I YACGTR 4 cut(s) 949, 4728, 5722, 8915
PpuMI RGGWCCY 3 cut(s) 1555, 4798, 7571
PscI ACATGT 1 cut(s) 4331
PshBI ATTAAT 3 cut(s) 714, 4863, 5378
PsiI TTATAA 2 cut(s) 7784, 8799
Psp124BI GAGCTC 1 cut(s) 5888
Psp5II RGGWCCY 3 cut(s) 1555, 4798, 7571
PspCI CACGTG 2 cut(s) 4728, 8915
PspEI GGTNACC 1 cut(s) 7670
PspFI CCCAGC 2 cut(s) 2614, 3648
PspOMI GGGCCC 1 cut(s) 8298
PspPI GGNCC 9 cut(s) 737, 1555, 2170, 3272, 4798, 5929, 7571, 8298, 8299
PspPPI RGGWCCY 3 cut(s) 1555, 4798, 7571
PstI CTGCAG 7 cut(s) 151, 1471, 1753, 3901, 6726, 6775, 8575
PstNI CAGNNNCTG 4 cut(s) 1046, 1589, 3073, 3676
PvuI CGATCG 1 cut(s) 9012
PvuII CAGCTG 7 cut(s) 862, 1043, 1754, 3442, 3648, 8074, 8453
SacI GAGCTC 1 cut(s) 5888
SalI GTCGAC 1 cut(s) 5620
SapI GCTCTTC 2 cut(s) 5007, 5733
Sau96I GGNCC 9 cut(s) 737, 1555, 2170, 3272, 4798, 5929, 7571, 8298, 8299
ScaI AGTACT 3 cut(s) 3420, 5939, 6421
SfcI CTRYAG 9 cut(s) 147, 1467, 1749, 3897, 4878, 6694, 6722, 6771, 8571
Sfr274I CTCGAG 2 cut(s) 63, 1618
SinI GGWCC 5 cut(s) 737, 1555, 3272, 4798, 7571
SlaI CTCGAG 2 cut(s) 63, 1618
SnaBI TACGTA 1 cut(s) 949
SpeI ACTAGT 1 cut(s) 8498
SphI GCATGC 3 cut(s) 1118, 4091, 7480
SseBI AGGCCT 2 cut(s) 2065, 8410
SspI AATATT 2 cut(s) 2124, 2722
SstI GAGCTC 1 cut(s) 5888
StuI AGGCCT 2 cut(s) 2065, 8410
TaqII GACCGA 1 cut(s) 7258
TatI WGTACW 7 cut(s) 507, 1048, 1227, 1596, 3418, 5937, 6419
TauI GCSGC 4 cut(s) 253, 3269, 5615, 6770
TspGWI ACGGA 7 cut(s) 2846, 3969, 4960, 6637, 6722, 6731, 8861
Van91I CCANNNNNTGG 1 cut(s) 8225
Vha464I CTTAAG 4 cut(s) 827, 2822, 6688, 8863
VneI GTGCAC 1 cut(s) 8916
VpaK11BI GGWCC 5 cut(s) 737, 1555, 3272, 4798, 7571
VspI ATTAAT 3 cut(s) 714, 4863, 5378
XagI CCTNNNNNAGG 2 cut(s) 1436, 6743
XbaI TCTAGA 1 cut(s) 6752
XceI RCATGY 6 cut(s) 1118, 4091, 4095, 4335, 6122, 7480
XcmI CCANNNNNNNNNTGG 5 cut(s) 3164, 6043, 8309, 8503, 8799
XhoI CTCGAG 2 cut(s) 63, 1618
XmaJI CCTAGG 1 cut(s) 968
XmiI GTMKAC 3 cut(s) 5621, 7061, 7084
XmnI GAANNNNTTC 7 cut(s) 661, 688, 2721, 5014, 5157, 8580, 8640
ZraI GACGTC 2 cut(s) 19, 5625
ZrmI AGTACT 3 cut(s) 3420, 5939, 6421
Zsp2I ATGCAT 5 cut(s) 2958, 3321, 4093, 6120, 8421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.