Rroxscaffold_4G00324420

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002934
Physical Location & Seq
Reverse (-)
55843913 .. 55849957
6045 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00324420.1

Sequence Viewer

Length: 483 bp
ATGGCACATAAAGTTATTAAAATCAACACATCCTTGGAGAAATTGATTAATATGGCAAGTGCTATTGAGTTGAGTGCTAAGCCGTCCATTTATGCAATGAGCTCCAATGGTCAAAGAATTGACCGGGAAACATACTCTCATTTTGAAGCTATAGTCCGATTTGAGCACACTCTTGTTCGAGGTTTGGATCCATCCCTTAATCCCTTCAAACCTCCACCACAAGCTGCTTTATCATCTTCAACGGTGGCCCCGCCGCTTGGAGTAGCCGTCCTTCTTCTTCGTCATGCGAGCAAACATGCTCTTAGCGATTCTGTTCCGATTGAGATCCGAAGCCTCGCAACAACAATCCTATTCTCGCAGACTTGGCCGCCGGAGAATTCAACGCCTTCCCATGGATCACTCTCATCGTCGTCACCGCTCCGTTGCTCCGAAGCTCTTCGACCTGCTTCGCTTTTGAGCCAAAGCCAAGGCCGACCGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 ko04014 map03460 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

160

Amino Acids

17.3

Weight (kDa)

9.8

Isoelectric Point (pI)

57.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 451
AccBSI CCGCTC 1 cut(s) 418
AccII CGCG 1 cut(s) 478
AciI CCGC 5 cut(s) 251, 254, 368, 416, 476
AclWI GGATC 4 cut(s) 182, 195, 319, 403
AcoI YGGCCR 1 cut(s) 365
AcsI RAATTY 1 cut(s) 376
AfiI CCNNNNNNNGG 2 cut(s) 257, 392
AgsI TTSAA 4 cut(s) 146, 208, 240, 381
AluBI AGCT 4 cut(s) 102, 149, 224, 434
AluI AGCT 4 cut(s) 102, 149, 224, 434
Alw21I GWGCWC 2 cut(s) 104, 168
AlwI GGATC 4 cut(s) 182, 195, 319, 403
AoxI GGCC 3 cut(s) 246, 365, 469
ApeKI GCWGC 1 cut(s) 224
ApoI RAATTY 1 cut(s) 376
AseI ATTAAT 1 cut(s) 48
Asp700I GAANNNNTTC 1 cut(s) 435
AspS9I GGNCC 1 cut(s) 247
AsuC2I CCSGG 1 cut(s) 125
AsuHPI GGTGA 1 cut(s) 405
BamHI GGATCC 1 cut(s) 187
BanII GRGCYC 1 cut(s) 104
BarI GAAGNNNNNNTAC 2 cut(s) 255, 287
Bbv12I GWGCWC 2 cut(s) 104, 168
BbvI GCAGC 1 cut(s) 211
BccI CCATC 1 cut(s) 199
BceAI ACGGC 2 cut(s) 67, 251
BcnI CCSGG 1 cut(s) 125
BfmI CTRYAG 1 cut(s) 150
BfuAI ACCTGC 1 cut(s) 451
BisI GCNGC 3 cut(s) 225, 254, 368
BlpI GCTNAGC 1 cut(s) 78
BlsI GCNGC 3 cut(s) 226, 255, 369
Bme1390I CCNGG 1 cut(s) 125
BmgT120I GGNCC 1 cut(s) 247
BmiI GGNNCC 2 cut(s) 189, 249
BmrFI CCNGG 1 cut(s) 125
Bpu1102I GCTNAGC 1 cut(s) 78
BpuMI CCSGG 1 cut(s) 125
BsaBI GATNNNNATC 1 cut(s) 323
BsaJI CCNNGG 3 cut(s) 33, 391, 466
BsaXI ACNNNNNCTCC 2 cut(s) 252, 282
Bsc4I CCNNNNNNNGG 2 cut(s) 257, 392
Bse3DI GCAATG 1 cut(s) 102
Bse8I GATNNNNATC 1 cut(s) 323
BseDI CCNNGG 3 cut(s) 33, 391, 466
BseGI GGATG 2 cut(s) 29, 191
BseJI GATNNNNATC 1 cut(s) 323
BseLI CCNNNNNNNGG 2 cut(s) 257, 392
BseMI GCAATG 1 cut(s) 102
BseXI GCAGC 1 cut(s) 211
Bsh1236I CGCG 1 cut(s) 478
Bsh1285I CGRYCG 1 cut(s) 476
BshFI GGCC 3 cut(s) 248, 367, 471
BsiEI CGRYCG 1 cut(s) 476
BsiHKAI GWGCWC 2 cut(s) 104, 168
BsiSI CCGG 2 cut(s) 124, 371
BslI CCNNNNNNNGG 2 cut(s) 257, 392
BsnI GGCC 3 cut(s) 248, 367, 471
Bsp1286I GDGCHC 2 cut(s) 104, 168
Bsp143I GATC 3 cut(s) 187, 324, 395
Bsp1720I GCTNAGC 1 cut(s) 78
Bsp19I CCATGG 1 cut(s) 391
BspACI CCGC 5 cut(s) 251, 254, 368, 416, 476
BspANI GGCC 3 cut(s) 248, 367, 471
BspFNI CGCG 1 cut(s) 478
BspLI GGNNCC 2 cut(s) 189, 249
BspMI ACCTGC 1 cut(s) 451
BspPI GGATC 4 cut(s) 182, 195, 319, 403
BspQI GCTCTTC 1 cut(s) 441
BsrBI CCGCTC 1 cut(s) 418
BsrDI GCAATG 1 cut(s) 102
BssECI CCNNGG 3 cut(s) 33, 391, 466
BssMI GATC 3 cut(s) 187, 324, 395
BssT1I CCWWGG 3 cut(s) 33, 391, 466
Bst4CI ACNGT 1 cut(s) 244
Bst6I CTCTTC 1 cut(s) 441
BstC8I GCNNGC 1 cut(s) 289
BstDEI CTNAG 2 cut(s) 78, 302
BstDSI CCRYGG 1 cut(s) 391
BstF5I GGATG 2 cut(s) 29, 191
BstFNI CGCG 1 cut(s) 478
BstKTI GATC 3 cut(s) 190, 327, 398
BstMBI GATC 3 cut(s) 187, 324, 395
BstMCI CGRYCG 1 cut(s) 476
BstMWI GCNNNNNNNGC 1 cut(s) 364
BstNSI RCATGY 1 cut(s) 299
BstSCI CCNGG 1 cut(s) 123
BstSFI CTRYAG 1 cut(s) 150
BstUI CGCG 1 cut(s) 478
BstV1I GCAGC 1 cut(s) 211
BstX2I RGATCY 2 cut(s) 187, 324
BstYI RGATCY 2 cut(s) 187, 324
BsuRI GGCC 3 cut(s) 248, 367, 471
BtgI CCRYGG 1 cut(s) 391
BtsCI GGATG 2 cut(s) 29, 191
BveI ACCTGC 1 cut(s) 451
Cac8I GCNNGC 1 cut(s) 289
Cfr13I GGNCC 1 cut(s) 247
CviAII CATG 3 cut(s) 284, 296, 392
DdeI CTNAG 2 cut(s) 78, 302
DpnI GATC 3 cut(s) 189, 326, 397
DpnII GATC 3 cut(s) 187, 324, 395
EaeI YGGCCR 1 cut(s) 365
Eam1104I CTCTTC 1 cut(s) 441
EarI CTCTTC 1 cut(s) 441
Ecl136II GAGCTC 1 cut(s) 102
Eco130I CCWWGG 3 cut(s) 33, 391, 466
Eco24I GRGCYC 1 cut(s) 104
Eco53kI GAGCTC 1 cut(s) 102
EcoICRI GAGCTC 1 cut(s) 102
EcoRI GAATTC 1 cut(s) 376
EcoT14I CCWWGG 3 cut(s) 33, 391, 466
EcoT38I GRGCYC 1 cut(s) 104
ErhI CCWWGG 3 cut(s) 33, 391, 466
FaeI CATG 3 cut(s) 287, 299, 395
FaiI YATR 8 cut(s) 9, 53, 93, 133, 152, 285, 297, 393
FatI CATG 3 cut(s) 283, 295, 391
FauI CCCGC 1 cut(s) 258
Fnu4HI GCNGC 3 cut(s) 225, 254, 368
FokI GGATG 2 cut(s) 16, 178
FriOI GRGCYC 1 cut(s) 104
Fsp4HI GCNGC 3 cut(s) 225, 254, 368
GluI GCNGC 3 cut(s) 225, 254, 368
HaeIII GGCC 3 cut(s) 248, 367, 471
HapII CCGG 2 cut(s) 124, 371
Hin1II CATG 3 cut(s) 287, 299, 395
HinfI GANTC 1 cut(s) 308
HpaII CCGG 2 cut(s) 124, 371
HphI GGTGA 1 cut(s) 405
Hpy188I TCNGA 4 cut(s) 158, 318, 329, 430
Hpy99I CGWCG 1 cut(s) 412
HpyAV CCTTC 3 cut(s) 214, 281, 396
HpyCH4III ACNGT 1 cut(s) 244
HpyCH4V TGCA 1 cut(s) 95
HpyF10VI GCNNNNNNNGC 1 cut(s) 364
HpyF3I CTNAG 2 cut(s) 78, 302
Hsp92II CATG 3 cut(s) 287, 299, 395
Kzo9I GATC 3 cut(s) 187, 324, 395
LguI GCTCTTC 1 cut(s) 441
LmnI GCTCC 3 cut(s) 107, 423, 431
LpnPI CCDG 3 cut(s) 137, 384, 456
Lsp1109I GCAGC 1 cut(s) 211
MaeIII GTNAC 1 cut(s) 411
MalI GATC 3 cut(s) 189, 326, 397
MbiI CCGCTC 1 cut(s) 418
MboI GATC 3 cut(s) 187, 324, 395
MboII GAAGA 4 cut(s) 228, 266, 269, 428
MflI RGATCY 2 cut(s) 187, 324
MhlI GDGCHC 2 cut(s) 104, 168
MluCI AATT 3 cut(s) 41, 117, 376
MnlI CCTC 3 cut(s) 173, 222, 344
MroXI GAANNNNTTC 1 cut(s) 435
MseI TTAA 4 cut(s) 18, 48, 198, 481
MspI CCGG 2 cut(s) 124, 371
MspR9I CCNGG 1 cut(s) 125
MvnI CGCG 1 cut(s) 478
MwoI GCNNNNNNNGC 1 cut(s) 364
NciI CCSGG 1 cut(s) 125
NcoI CCATGG 1 cut(s) 391
NdeII GATC 3 cut(s) 187, 324, 395
NlaIII CATG 3 cut(s) 287, 299, 395
NlaIV GGNNCC 2 cut(s) 189, 249
NmuCI GTSAC 1 cut(s) 411
NspI RCATGY 1 cut(s) 299
PciSI GCTCTTC 1 cut(s) 441
PdmI GAANNNNTTC 1 cut(s) 435
PfeI GAWTC 1 cut(s) 308
PkrI GCNGC 3 cut(s) 226, 255, 369
PshBI ATTAAT 1 cut(s) 48
Psp124BI GAGCTC 1 cut(s) 104
PspN4I GGNNCC 2 cut(s) 189, 249
PspPI GGNCC 1 cut(s) 247
PsuI RGATCY 2 cut(s) 187, 324
SacI GAGCTC 1 cut(s) 104
SapI GCTCTTC 1 cut(s) 441
SaqAI TTAA 4 cut(s) 18, 48, 198, 481
SatI GCNGC 3 cut(s) 225, 254, 368
Sau3AI GATC 3 cut(s) 187, 324, 395
Sau96I GGNCC 1 cut(s) 247
ScrFI CCNGG 1 cut(s) 125
SduI GDGCHC 2 cut(s) 104, 168
SetI ASST 7 cut(s) 104, 151, 184, 214, 226, 436, 445
SfcI CTRYAG 1 cut(s) 150
Sse9I AATT 3 cut(s) 41, 117, 376
SsiI CCGC 5 cut(s) 251, 254, 368, 416, 476
SstI GAGCTC 1 cut(s) 104
StyD4I CCNGG 1 cut(s) 123
StyI CCWWGG 3 cut(s) 33, 391, 466
TaaI ACNGT 1 cut(s) 244
TaqI TCGA 2 cut(s) 178, 439
TasI AATT 3 cut(s) 41, 117, 376
TauI GCSGC 2 cut(s) 256, 370
TfiI GAWTC 1 cut(s) 308
Tru1I TTAA 4 cut(s) 18, 48, 198, 481
Tru9I TTAA 4 cut(s) 18, 48, 198, 481
TseFI GTSAC 1 cut(s) 411
TseI GCWGC 1 cut(s) 224
Tsp45I GTSAC 1 cut(s) 411
TspGWI ACGGA 1 cut(s) 410
VspI ATTAAT 1 cut(s) 48
XapI RAATTY 1 cut(s) 376
XceI RCATGY 1 cut(s) 299
XmnI GAANNNNTTC 1 cut(s) 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.