MD06G1182300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0011067
Physical Location & Seq
Reverse (-)
32099011 .. 32101593
2583 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1182300.v1.1.491

Sequence Viewer

Length: 1032 bp
ATGGAGGAGGAAAAAGCGGCGGCGTACTACGACGAGCTGACTCGGCGCGGCGAAGGAGCCGCCCGATTCAAACAAGGCCTCGGCTTTTCCTCCGCAAACTCGGATTCCGAAAATACCCCTGTGCGCGGCTCAGCGCTGGCGTACTCCACCTCGTCCTCCTTCCTCAGTAAGTTCGTTAAGGCGTCCAGCCCGAAGAAAGAGTCGGAGCTCCGAAATCAGGCCCAAATCGAGTCCATACAGAACAAGCTCAAGAGGAAGAGGCCCGACGCGGCGGAGGAAAGAGAAGAGAAACAACCGGCTAGGGTTTCGCAGAGAGATAATAAGCATTCGAGGAAGCGGAGCAGGAGCAGGAGCAGAGAGAGGCATTCGAGGCGGAGAAGTAGGAGCAGAGAGAGACATAGAGAAAGAGATAGGGTTAGAGAGAGAAGGAAGAGGAGTAGGTCCGGGAGCGATTCCGATGGAGGTAGACGACGGAGAGGGAGGAGCCGGAGTCGGAGCTCGTCGCCGAGGGAGAGGGTTTCGGAGAGGACCAAAGGTAAATCGGGGAAGGAGAGGAAAGGCGGCGGTGCTGATTATTCCAAGCTGATTGAAGGCTATGATAAGATGTCCGCGGCCGAGAGAGTGAAGGCGAAGATGAAGCTTCAACTTGCCGAAACTTCTGAGAAGGATGCAGCGAAAGGGATGGGCTCGGGTTGGGAAAGGTTCGAGTTCAACAAAGATGCACCACTTGATGATGAAGAAGTTGAAGCTGCTGATGATGATGCAGCACTAGTTAAGCACATTGGCCAAAGCTTCCGGTTTTCTGCAGTTGAGACAAAAAGGGAGGAGCAGCTCAGATCTGCTCATGATGAGGCTATGTTTGGTGCATCAGATGATCGACCGACCATTGTGATGGATAGTGAAATTGAGGCAGAGAACGACACAAAGGATAGCAAAGAAAGTGAGCCTACAACTACAGTCCTCTTGAGTGATACGGTACTTGCAAAGAAACAAGGTTCATGGCGTGATCGTATACGTGACAAAAGGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

344

Amino Acids

39.12

Weight (kDa)

10.0

Isoelectric Point (pI)

83.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011067)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48120 AT3G48120
fragaria_vesca FvH4_5g16290
malus_domestica MD06G1182300.v1.1 MD14G1188300.v1.1
prunus_persica Prupe.5G180400_v2.0.a1
pyrus_communis pycom06g16120
rosa_chinensis RchiOBHm_Chr7g0179841
rosa_laevigata RLG00000005319
rosa_multiflora Rmu_sc0001754.1_g000002 Rmu_sc0004156.1_g000013 Rmu_sc0035735.1_g000001
rosa_roxburghii Rroxscaffold_3G00273410
rosa_rugosa Rorug06G0428800
rosa_samantha Rh7AG028500 Rh7BG027500 Rh7BG027600 Rh7CG029400 Rh7DG028800
rosa_wichuraiana Rw7G002280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 466, 1012
AccII CGCG 4 cut(s) 48, 126, 269, 611
AcoI YGGCCR 2 cut(s) 612, 784
AcyI GRCGYC 1 cut(s) 182
AfaI GTAC 3 cut(s) 26, 143, 978
AfeI AGCGCT 1 cut(s) 135
AfiI CCNNNNNNNGG 2 cut(s) 125, 217
AgsI TTSAA 5 cut(s) 70, 590, 644, 712, 746
AhlI ACTAGT 1 cut(s) 769
AluBI AGCT 9 cut(s) 37, 208, 247, 498, 583, 640, 749, 792, 832
AluI AGCT 9 cut(s) 37, 208, 247, 498, 583, 640, 749, 792, 832
Alw21I GWGCWC 2 cut(s) 210, 500
Alw26I GTCTC 2 cut(s) 388, 806
Ama87I CYCGRG 1 cut(s) 688
Aor51HI AGCGCT 1 cut(s) 135
AoxI GGCC 5 cut(s) 76, 219, 260, 612, 784
ApeKI GCWGC 4 cut(s) 671, 749, 764, 829
ArsI GACNNNNNNTTYG 2 cut(s) 185, 217
AspLEI GCGC 3 cut(s) 48, 126, 136
AspS9I GGNCC 4 cut(s) 220, 261, 441, 528
AsuC2I CCSGG 1 cut(s) 445
AvaI CYCGRG 1 cut(s) 688
AvaII GGWCC 2 cut(s) 441, 528
BalI TGGCCA 1 cut(s) 786
BanII GRGCYC 3 cut(s) 210, 500, 689
Bbv12I GWGCWC 2 cut(s) 210, 500
BbvI GCAGC 4 cut(s) 683, 736, 776, 841
BccI CCATC 3 cut(s) 452, 676, 886
BcnI CCSGG 1 cut(s) 445
BcoDI GTCTC 2 cut(s) 388, 806
BcuI ACTAGT 1 cut(s) 769
BfaI CTAG 2 cut(s) 300, 770
BfmI CTRYAG 2 cut(s) 804, 954
BfoI RGCGCY 1 cut(s) 137
BglII AGATCT 1 cut(s) 836
BlpI GCTNAGC 1 cut(s) 130
Bme1390I CCNGG 1 cut(s) 445
Bme18I GGWCC 2 cut(s) 441, 528
BmeT110I CYCGRG 1 cut(s) 688
BmgT120I GGNCC 4 cut(s) 220, 261, 441, 528
BmiI GGNNCC 2 cut(s) 58, 485
BmrFI CCNGG 1 cut(s) 445
BmsI GCATC 4 cut(s) 658, 709, 751, 875
Bpu1102I GCTNAGC 1 cut(s) 130
BpuEI CTTGAG 2 cut(s) 233, 985
BpuMI CCSGG 1 cut(s) 445
BsaAI YACGTR 1 cut(s) 1016
BsaHI GRCGYC 1 cut(s) 182
BsaJI CCNNGG 3 cut(s) 79, 506, 609
BsaWI WCCGGW 1 cut(s) 795
BsaXI ACNNNNNCTCC 2 cut(s) 475, 505
Bsc4I CCNNNNNNNGG 2 cut(s) 125, 217
Bse118I RCCGGY 1 cut(s) 295
BseDI CCNNGG 3 cut(s) 79, 506, 609
BseGI GGATG 2 cut(s) 673, 687
BseLI CCNNNNNNNGG 2 cut(s) 125, 217
BseMII CTCAG 4 cut(s) 144, 178, 651, 847
BseRI GAGGAG 4 cut(s) 20, 448, 496, 839
BseX3I CGGCCG 1 cut(s) 612
BseXI GCAGC 4 cut(s) 683, 736, 776, 841
Bsh1236I CGCG 4 cut(s) 48, 126, 269, 611
Bsh1285I CGRYCG 2 cut(s) 615, 881
BshFI GGCC 5 cut(s) 78, 221, 262, 614, 786
BsiEI CGRYCG 2 cut(s) 615, 881
BsiHKAI GWGCWC 2 cut(s) 210, 500
BsiHKCI CYCGRG 1 cut(s) 688
BsiSI CCGG 4 cut(s) 296, 444, 487, 796
BslI CCNNNNNNNGG 2 cut(s) 125, 217
BsmAI GTCTC 2 cut(s) 388, 806
BsmI GAATGC 2 cut(s) 325, 364
BsnI GGCC 5 cut(s) 78, 221, 262, 614, 786
BsoBI CYCGRG 1 cut(s) 688
Bsp1286I GDGCHC 3 cut(s) 210, 500, 689
Bsp143I GATC 3 cut(s) 836, 874, 1006
Bsp1720I GCTNAGC 1 cut(s) 130
BspANI GGCC 5 cut(s) 78, 221, 262, 614, 786
BspCNI CTCAG 4 cut(s) 143, 177, 652, 846
BspFNI CGCG 4 cut(s) 48, 126, 269, 611
BspHI TCATGA 1 cut(s) 844
BspLI GGNNCC 2 cut(s) 58, 485
BspMAI CTGCAG 1 cut(s) 808
BsrFI RCCGGY 1 cut(s) 295
BssAI RCCGGY 1 cut(s) 295
BssECI CCNNGG 3 cut(s) 79, 506, 609
BssMI GATC 3 cut(s) 836, 874, 1006
BssNAI GTATAC 1 cut(s) 1013
BssNI GRCGYC 1 cut(s) 182
Bst1107I GTATAC 1 cut(s) 1013
Bst4CI ACNGT 2 cut(s) 958, 976
Bst6I CTCTTC 3 cut(s) 251, 279, 425
BstACI GRCGYC 1 cut(s) 182
BstBAI YACGTR 1 cut(s) 1016
BstC8I GCNNGC 1 cut(s) 138
BstDEI CTNAG 4 cut(s) 130, 164, 660, 833
BstDSI CCRYGG 1 cut(s) 609
BstF5I GGATG 2 cut(s) 673, 687
BstFNI CGCG 4 cut(s) 48, 126, 269, 611
BstH2I RGCGCY 1 cut(s) 137
BstHHI GCGC 3 cut(s) 48, 126, 136
BstKTI GATC 3 cut(s) 839, 877, 1009
BstMAI GTCTC 2 cut(s) 388, 806
BstMBI GATC 3 cut(s) 836, 874, 1006
BstMCI CGRYCG 2 cut(s) 615, 881
BstMWI GCNNNNNNNGC 2 cut(s) 43, 370
BstSCI CCNGG 1 cut(s) 443
BstSFI CTRYAG 2 cut(s) 804, 954
BstUI CGCG 4 cut(s) 48, 126, 269, 611
BstV1I GCAGC 4 cut(s) 683, 736, 776, 841
BstX2I RGATCY 1 cut(s) 836
BstXI CCANNNNNNTGG 1 cut(s) 892
BstYI RGATCY 1 cut(s) 836
BstZ17I GTATAC 1 cut(s) 1013
BstZI CGGCCG 1 cut(s) 612
BsuRI GGCC 5 cut(s) 78, 221, 262, 614, 786
BtgI CCRYGG 1 cut(s) 609
BtsCI GGATG 2 cut(s) 673, 687
Cac8I GCNNGC 1 cut(s) 138
CciI TCATGA 1 cut(s) 844
CfoI GCGC 3 cut(s) 48, 126, 136
Cfr10I RCCGGY 1 cut(s) 295
Cfr13I GGNCC 4 cut(s) 220, 261, 441, 528
Cfr42I CCGCGG 1 cut(s) 612
CseI GACGC 2 cut(s) 171, 275
Csp6I GTAC 3 cut(s) 25, 142, 977
CviAII CATG 2 cut(s) 845, 999
CviQI GTAC 3 cut(s) 25, 142, 977
DdeI CTNAG 4 cut(s) 130, 164, 660, 833
DpnI GATC 3 cut(s) 838, 876, 1008
DpnII GATC 3 cut(s) 836, 874, 1006
EaeI YGGCCR 2 cut(s) 612, 784
EagI CGGCCG 1 cut(s) 612
Eam1104I CTCTTC 3 cut(s) 251, 279, 425
EarI CTCTTC 3 cut(s) 251, 279, 425
EciI GGCGGA 2 cut(s) 287, 388
Ecl136II GAGCTC 2 cut(s) 208, 498
EclXI CGGCCG 1 cut(s) 612
Eco147I AGGCCT 1 cut(s) 78
Eco24I GRGCYC 3 cut(s) 210, 500, 689
Eco47I GGWCC 2 cut(s) 441, 528
Eco47III AGCGCT 1 cut(s) 135
Eco52I CGGCCG 1 cut(s) 612
Eco53kI GAGCTC 2 cut(s) 208, 498
Eco88I CYCGRG 1 cut(s) 688
EcoICRI GAGCTC 2 cut(s) 208, 498
EcoT38I GRGCYC 3 cut(s) 210, 500, 689
FaeI CATG 2 cut(s) 848, 1002
FaiI YATR 7 cut(s) 236, 399, 597, 846, 857, 1000, 1013
FatI CATG 2 cut(s) 844, 998
FblI GTMKAC 2 cut(s) 466, 1012
FokI GGATG 2 cut(s) 680, 694
FriOI GRGCYC 3 cut(s) 210, 500, 689
FspBI CTAG 2 cut(s) 300, 770
GlaI GCGC 3 cut(s) 47, 125, 135
HaeII RGCGCY 1 cut(s) 137
HaeIII GGCC 5 cut(s) 78, 221, 262, 614, 786
HapII CCGG 4 cut(s) 296, 444, 487, 796
HgaI GACGC 2 cut(s) 171, 275
HhaI GCGC 3 cut(s) 48, 126, 136
Hin1I GRCGYC 1 cut(s) 182
Hin1II CATG 2 cut(s) 848, 1002
Hin6I GCGC 3 cut(s) 46, 124, 134
HinP1I GCGC 3 cut(s) 46, 124, 134
HindIII AAGCTT 2 cut(s) 638, 790
HinfI GANTC 7 cut(s) 40, 66, 104, 200, 230, 452, 490
HpaII CCGG 4 cut(s) 296, 444, 487, 796
Hpy166II GTNNAC 2 cut(s) 467, 1013
Hpy188III TCNNGA 3 cut(s) 250, 845, 964
Hpy8I GTNNAC 2 cut(s) 467, 1013
Hpy99I CGWCG 4 cut(s) 35, 269, 474, 505
HpyAV CCTTC 7 cut(s) 47, 169, 420, 541, 584, 619, 658
HpyCH4III ACNGT 2 cut(s) 958, 976
HpyCH4IV ACGT 1 cut(s) 1015
HpyCH4V TGCA 6 cut(s) 671, 722, 764, 806, 866, 983
HpyF10VI GCNNNNNNNGC 2 cut(s) 43, 370
HpyF3I CTNAG 4 cut(s) 130, 164, 660, 833
HpySE526I ACGT 1 cut(s) 1015
Hsp92I GRCGYC 1 cut(s) 182
Hsp92II CATG 2 cut(s) 848, 1002
HspAI GCGC 3 cut(s) 46, 124, 134
KspI CCGCGG 1 cut(s) 612
Kzo9I GATC 3 cut(s) 836, 874, 1006
Lsp1109I GCAGC 4 cut(s) 683, 736, 776, 841
LweI GCATC 4 cut(s) 658, 709, 751, 875
MaeI CTAG 2 cut(s) 300, 770
MaeII ACGT 1 cut(s) 1015
MaeIII GTNAC 1 cut(s) 1016
MalI GATC 3 cut(s) 838, 876, 1008
MboI GATC 3 cut(s) 836, 874, 1006
MboII GAAGA 6 cut(s) 205, 268, 296, 442, 643, 749
MflI RGATCY 1 cut(s) 836
MhlI GDGCHC 3 cut(s) 210, 500, 689
MlsI TGGCCA 1 cut(s) 786
MluCI AATT 2 cut(s) 903, 1027
MluNI TGGCCA 1 cut(s) 786
MlyI GAGTC 4 cut(s) 34, 209, 239, 499
MmeI TCCRAC 2 cut(s) 183, 473
Mox20I TGGCCA 1 cut(s) 786
MscI TGGCCA 1 cut(s) 786
MseI TTAA 3 cut(s) 177, 774, 1030
MslI CAYNNNNRTG 1 cut(s) 890
Msp20I TGGCCA 1 cut(s) 786
MspA1I CMGCKG 1 cut(s) 611
MspI CCGG 4 cut(s) 296, 444, 487, 796
MspR9I CCNGG 1 cut(s) 445
Mva1269I GAATGC 2 cut(s) 325, 364
MvnI CGCG 4 cut(s) 48, 126, 269, 611
MwoI GCNNNNNNNGC 2 cut(s) 43, 370
NciI CCSGG 1 cut(s) 445
NdeII GATC 3 cut(s) 836, 874, 1006
NlaIII CATG 2 cut(s) 848, 1002
NlaIV GGNNCC 2 cut(s) 58, 485
NmeAIII GCCGAG 4 cut(s) 22, 60, 531, 640
NmuCI GTSAC 1 cut(s) 1016
PagI TCATGA 1 cut(s) 844
PceI AGGCCT 1 cut(s) 78
PctI GAATGC 2 cut(s) 325, 364
PfeI GAWTC 3 cut(s) 66, 104, 452
PfoI TCCNGGA 1 cut(s) 443
PleI GAGTC 4 cut(s) 34, 208, 238, 498
PpsI GAGTC 4 cut(s) 34, 208, 238, 498
Ppu21I YACGTR 1 cut(s) 1016
Psp124BI GAGCTC 2 cut(s) 210, 500
PspN4I GGNNCC 2 cut(s) 58, 485
PspPI GGNCC 4 cut(s) 220, 261, 441, 528
PstI CTGCAG 1 cut(s) 808
PsuI RGATCY 1 cut(s) 836
RsaI GTAC 3 cut(s) 26, 143, 978
RsaNI GTAC 3 cut(s) 25, 142, 977
RseI CAYNNNNRTG 1 cut(s) 890
SacI GAGCTC 2 cut(s) 210, 500
SacII CCGCGG 1 cut(s) 612
SaqAI TTAA 3 cut(s) 177, 774, 1030
Sau3AI GATC 3 cut(s) 836, 874, 1006
Sau96I GGNCC 4 cut(s) 220, 261, 441, 528
SchI GAGTC 4 cut(s) 34, 209, 239, 499
ScrFI CCNGG 1 cut(s) 445
SduI GDGCHC 3 cut(s) 210, 500, 689
SfaNI GCATC 4 cut(s) 658, 709, 751, 875
SfcI CTRYAG 2 cut(s) 804, 954
Sfr303I CCGCGG 1 cut(s) 612
SgrBI CCGCGG 1 cut(s) 612
SinI GGWCC 2 cut(s) 441, 528
SmiMI CAYNNNNRTG 1 cut(s) 890
SmlI CTYRAG 2 cut(s) 248, 964
SmoI CTYRAG 2 cut(s) 248, 964
SpeI ACTAGT 1 cut(s) 769
Sse9I AATT 2 cut(s) 903, 1027
SseBI AGGCCT 1 cut(s) 78
SspMI CTAG 2 cut(s) 300, 770
SstI GAGCTC 2 cut(s) 210, 500
StuI AGGCCT 1 cut(s) 78
StyD4I CCNGG 1 cut(s) 443
TaaI ACNGT 2 cut(s) 958, 976
TaiI ACGT 1 cut(s) 1018
TaqI TCGA 5 cut(s) 228, 329, 368, 705, 877
TaqII GACCGA 1 cut(s) 895
TasI AATT 2 cut(s) 903, 1027
TauI GCSGC 8 cut(s) 20, 23, 51, 62, 129, 272, 564, 614
TfiI GAWTC 3 cut(s) 66, 104, 452
Tru1I TTAA 3 cut(s) 177, 774, 1030
Tru9I TTAA 3 cut(s) 177, 774, 1030
TseFI GTSAC 1 cut(s) 1016
TseI GCWGC 4 cut(s) 671, 749, 764, 829
Tsp45I GTSAC 1 cut(s) 1016
TspDTI ATGAA 3 cut(s) 650, 750, 987
TspGWI ACGGA 1 cut(s) 487
VpaK11BI GGWCC 2 cut(s) 441, 528
XmiI GTMKAC 2 cut(s) 466, 1012
XspI CTAG 2 cut(s) 300, 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.