RLG00000005319

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0011067
Physical Location & Seq
Forward (+)
66754174 .. 66756483
2310 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005319

Sequence Viewer

Length: 1041 bp
ATGGAGGAAGCAAAAGCGGCGGCGTACTACGAAGACCTAACCCGGCGCGGCGAAGGCGCCGCCAGATTCAAACAAGGCCTCGGCTTCTCATCGGCGAAGTCCGATTCCGAAAATGCCCCTGCGCGCGGTTCGGCGGTGGCGTATACTTCCTCGTCTTCCTTCGTGAGCAAATTCGTCAAGGCCTCCAGCCCTAGCAAGACGACGGAGGTCGAAAAGCAAGCGCAGATCCAGTCGATTCAGAACAAGCTGAAGAGGAAGAAGCCGGAGGAGCTGGCCGGAGAGGAGGAGAAGCGGCGGCCTAGGGTTTCGGAGAGGGATAAGCGGAGTAGAAGCAGAGAGAGGGAGTCGAGGCGTCGGAGTAGGAGCAGAGAGAGGTATAGAGATAGGGAGAGAGATAGAGAGAGAACGAGGAGGAGGAGGAGCAGGTCTGGGAGTGAATCGGACGGAGATAGGAGACGAAGGAGTCGGTCGCCTCGGAATCGGAGAAAGGAACGGCGGAGGAGCCGGAGCCGGAGCTCGTCGCCGCCGAGGGAGAGGGGTTTGGAGAAGAGCAGAGGTAAATTGGGGAAGGAGAGGAGTGGCGGTGGTGCTGACTATTACAAATTGATTCAAGGCTATGACAAAATGTCTGCAGCGGAGAGAGTAAAAGCGAAGATGAAACTCCAACTTGCTGAAACGGCTGAGAAGGACACAACCAAAGGCATGGATGGTTGGGAAAGATTTGAATTCGATAAAGATGCACCACTAGACGATGAGGAGATTGAAGTTGCTGAAGATGATGCAGCGCTCGTCAATCACCTAGGCAAAAGCTTTCGGTTTTCAGCATTTGAGACAAAAAGGGAGGAGCGGATCAAAGCTGCTCATGATGAGGCAATGTTTGGTGCATCAGATAATCAACCATCCATTATGATAGACAGTGACGTTGAGGCAGAGAATGATAATAAGGACTGCAGAGAAAGTGAGCCTCCAATCCTCTTGAGTGAGAAGGTACTTGCCAAAAAACAAGGTTCATGGCGTGATCGTATACGCCACAACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

347

Amino Acids

39.92

Weight (kDa)

10.0

Isoelectric Point (pI)

87.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011067)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48120 AT3G48120
fragaria_vesca FvH4_5g16290
malus_domestica MD06G1182300.v1.1 MD14G1188300.v1.1
prunus_persica Prupe.5G180400_v2.0.a1
pyrus_communis pycom06g16120
rosa_chinensis RchiOBHm_Chr7g0179841
rosa_laevigata RLG00000005319
rosa_multiflora Rmu_sc0001754.1_g000002 Rmu_sc0004156.1_g000013 Rmu_sc0035735.1_g000001
rosa_roxburghii Rroxscaffold_3G00273410
rosa_rugosa Rorug06G0428800
rosa_samantha Rh7AG028500 Rh7BG027500 Rh7BG027600 Rh7CG029400 Rh7DG028800
rosa_wichuraiana Rw7G002280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 462
Acc36I ACCTGC 1 cut(s) 414
AccB1I GGYRCC 1 cut(s) 56
AccBSI CCGCTC 1 cut(s) 847
AccI GTMKAC 2 cut(s) 143, 1024
AccII CGCG 3 cut(s) 48, 124, 126
AclWI GGATC 2 cut(s) 220, 857
AcoI YGGCCR 1 cut(s) 273
AcsI RAATTY 2 cut(s) 170, 725
AcuI CTGAAG 2 cut(s) 269, 792
AcyI GRCGYC 2 cut(s) 57, 352
AfaI GTAC 2 cut(s) 26, 990
AfeI AGCGCT 1 cut(s) 786
AfiI CCNNNNNNNGG 1 cut(s) 125
AgsI TTSAA 4 cut(s) 70, 611, 725, 764
AhdI GACNNNNNGTC 1 cut(s) 625
AluBI AGCT 5 cut(s) 247, 271, 516, 810, 857
AluI AGCT 5 cut(s) 247, 271, 516, 810, 857
Alw21I GWGCWC 1 cut(s) 518
Alw26I GTCTC 2 cut(s) 448, 824
AlwI GGATC 2 cut(s) 220, 857
Aor51HI AGCGCT 1 cut(s) 786
AoxI GGCC 4 cut(s) 76, 180, 273, 296
ApeKI GCWGC 3 cut(s) 632, 782, 857
ApoI RAATTY 2 cut(s) 170, 725
AspA2I CCTAGG 2 cut(s) 299, 799
AspLEI GCGC 6 cut(s) 48, 59, 124, 126, 223, 787
AsuC2I CCSGG 1 cut(s) 43
AsuHPI GGTGA 1 cut(s) 788
AvrII CCTAGG 2 cut(s) 299, 799
BanI GGYRCC 1 cut(s) 56
BanII GRGCYC 1 cut(s) 518
BbsI GAAGAC 2 cut(s) 39, 147
Bbv12I GWGCWC 1 cut(s) 518
BbvI GCAGC 3 cut(s) 644, 794, 844
BccI CCATC 2 cut(s) 701, 907
BceAI ACGGC 2 cut(s) 509, 693
BcgI CGANNNNNNTGC 2 cut(s) 719, 753
BcnI CCSGG 1 cut(s) 43
BcoDI GTCTC 2 cut(s) 448, 824
BfaI CTAG 4 cut(s) 192, 300, 746, 800
BfmI CTRYAG 2 cut(s) 630, 949
BfoI RGCGCY 2 cut(s) 60, 788
BfuAI ACCTGC 1 cut(s) 414
BlnI CCTAGG 2 cut(s) 299, 799
Bme1390I CCNGG 1 cut(s) 43
BmeRI GACNNNNNGTC 1 cut(s) 625
BmiI GGNNCC 3 cut(s) 58, 503, 509
BmrFI CCNGG 1 cut(s) 43
BmsI GCATC 3 cut(s) 727, 769, 893
BoxI GACNNNNGTC 1 cut(s) 206
BpiI GAAGAC 2 cut(s) 39, 147
BpmI CTGGAG 1 cut(s) 169
BpuEI CTTGAG 1 cut(s) 997
BpuMI CCSGG 1 cut(s) 43
BsaHI GRCGYC 2 cut(s) 57, 352
BsaJI CCNNGG 5 cut(s) 79, 299, 473, 527, 799
BsaXI ACNNNNNCTCC 2 cut(s) 568, 598
Bsc4I CCNNNNNNNGG 1 cut(s) 125
Bse1I ACTGG 1 cut(s) 229
Bse3DI GCAATG 1 cut(s) 879
BseDI CCNNGG 5 cut(s) 79, 299, 473, 527, 799
BseGI GGATG 2 cut(s) 712, 899
BseLI CCNNNNNNNGG 1 cut(s) 125
BseMI GCAATG 1 cut(s) 879
BseMII CTCAG 1 cut(s) 672
BseNI ACTGG 1 cut(s) 229
BsePI GCGCGC 1 cut(s) 122
BseXI GCAGC 3 cut(s) 644, 794, 844
Bsh1236I CGCG 3 cut(s) 48, 124, 126
Bsh1285I CGRYCG 1 cut(s) 470
BshFI GGCC 4 cut(s) 78, 182, 275, 298
BshNI GGYRCC 1 cut(s) 56
BsiEI CGRYCG 1 cut(s) 470
BsiHKAI GWGCWC 1 cut(s) 518
BsiSI CCGG 5 cut(s) 43, 263, 276, 505, 511
BslI CCNNNNNNNGG 1 cut(s) 125
BsmAI GTCTC 2 cut(s) 448, 824
BsmBI CGTCTC 1 cut(s) 448
BsnI GGCC 4 cut(s) 78, 182, 275, 298
Bsp1286I GDGCHC 1 cut(s) 518
Bsp143I GATC 3 cut(s) 225, 849, 1018
BspANI GGCC 4 cut(s) 78, 182, 275, 298
BspCNI CTCAG 1 cut(s) 673
BspFNI CGCG 3 cut(s) 48, 124, 126
BspHI TCATGA 1 cut(s) 862
BspLI GGNNCC 3 cut(s) 58, 503, 509
BspMAI CTGCAG 2 cut(s) 634, 953
BspMI ACCTGC 1 cut(s) 414
BspPI GGATC 2 cut(s) 220, 857
BspQI GCTCTTC 1 cut(s) 542
BspT107I GGYRCC 1 cut(s) 56
BsrBI CCGCTC 1 cut(s) 847
BsrDI GCAATG 1 cut(s) 879
BsrI ACTGG 1 cut(s) 229
BssECI CCNNGG 5 cut(s) 79, 299, 473, 527, 799
BssHII GCGCGC 1 cut(s) 122
BssMI GATC 3 cut(s) 225, 849, 1018
BssNAI GTATAC 2 cut(s) 144, 1025
BssNI GRCGYC 2 cut(s) 57, 352
BssT1I CCWWGG 2 cut(s) 299, 799
Bst1107I GTATAC 2 cut(s) 144, 1025
Bst4CI ACNGT 1 cut(s) 917
Bst6I CTCTTC 2 cut(s) 245, 542
BstACI GRCGYC 2 cut(s) 57, 352
BstC8I GCNNGC 3 cut(s) 124, 219, 273
BstDEI CTNAG 1 cut(s) 681
BstF5I GGATG 2 cut(s) 712, 899
BstFNI CGCG 3 cut(s) 48, 124, 126
BstH2I RGCGCY 2 cut(s) 60, 788
BstHHI GCGC 6 cut(s) 48, 59, 124, 126, 223, 787
BstKTI GATC 3 cut(s) 228, 852, 1021
BstMAI GTCTC 2 cut(s) 448, 824
BstMBI GATC 3 cut(s) 225, 849, 1018
BstMCI CGRYCG 1 cut(s) 470
BstMWI GCNNNNNNNGC 4 cut(s) 17, 54, 268, 677
BstPAI GACNNNNGTC 1 cut(s) 206
BstSCI CCNGG 1 cut(s) 41
BstSFI CTRYAG 2 cut(s) 630, 949
BstUI CGCG 3 cut(s) 48, 124, 126
BstV1I GCAGC 3 cut(s) 644, 794, 844
BstV2I GAAGAC 2 cut(s) 39, 147
BstX2I RGATCY 1 cut(s) 225
BstXI CCANNNNNNTGG 1 cut(s) 703
BstYI RGATCY 1 cut(s) 225
BstZ17I GTATAC 2 cut(s) 144, 1025
BsuRI GGCC 4 cut(s) 78, 182, 275, 298
BtsCI GGATG 2 cut(s) 712, 899
BtsIMutI CAGTG 1 cut(s) 922
BveI ACCTGC 1 cut(s) 414
Cac8I GCNNGC 3 cut(s) 124, 219, 273
CciI TCATGA 1 cut(s) 862
CfoI GCGC 6 cut(s) 48, 59, 124, 126, 223, 787
CseI GACGC 1 cut(s) 341
Csp6I GTAC 2 cut(s) 25, 989
CspCI CAANNNNNGTGG 1 cut(s) 1019
CviAII CATG 3 cut(s) 703, 863, 1011
CviQI GTAC 2 cut(s) 25, 989
DdeI CTNAG 1 cut(s) 681
DinI GGCGCC 1 cut(s) 58
DpnI GATC 3 cut(s) 227, 851, 1020
DpnII GATC 3 cut(s) 225, 849, 1018
DrdI GACNNNNNNGTC 1 cut(s) 462
DriI GACNNNNNGTC 1 cut(s) 625
DseDI GACNNNNNNGTC 1 cut(s) 462
EaeI YGGCCR 1 cut(s) 273
Eam1104I CTCTTC 2 cut(s) 245, 542
Eam1105I GACNNNNNGTC 1 cut(s) 625
EarI CTCTTC 2 cut(s) 245, 542
EciI GGCGGA 1 cut(s) 511
Ecl136II GAGCTC 1 cut(s) 516
Eco130I CCWWGG 2 cut(s) 299, 799
Eco147I AGGCCT 2 cut(s) 78, 182
Eco24I GRGCYC 1 cut(s) 518
Eco47III AGCGCT 1 cut(s) 786
Eco53kI GAGCTC 1 cut(s) 516
Eco57I CTGAAG 2 cut(s) 269, 792
EcoICRI GAGCTC 1 cut(s) 516
EcoRI GAATTC 1 cut(s) 725
EcoT14I CCWWGG 2 cut(s) 299, 799
EcoT38I GRGCYC 1 cut(s) 518
EgeI GGCGCC 1 cut(s) 58
EheI GGCGCC 1 cut(s) 58
ErhI CCWWGG 2 cut(s) 299, 799
Esp3I CGTCTC 1 cut(s) 448
FaeI CATG 3 cut(s) 706, 866, 1014
FaiI YATR 8 cut(s) 144, 378, 618, 704, 864, 908, 1012, 1025
FatI CATG 3 cut(s) 702, 862, 1010
FblI GTMKAC 2 cut(s) 143, 1024
FokI GGATG 2 cut(s) 719, 886
FriOI GRGCYC 1 cut(s) 518
FspBI CTAG 4 cut(s) 192, 300, 746, 800
GlaI GCGC 6 cut(s) 47, 58, 123, 125, 222, 786
GsuI CTGGAG 1 cut(s) 169
HaeII RGCGCY 2 cut(s) 60, 788
HaeIII GGCC 4 cut(s) 78, 182, 275, 298
HapII CCGG 5 cut(s) 43, 263, 276, 505, 511
HgaI GACGC 1 cut(s) 341
HhaI GCGC 6 cut(s) 48, 59, 124, 126, 223, 787
Hin1I GRCGYC 2 cut(s) 57, 352
Hin1II CATG 3 cut(s) 706, 866, 1014
Hin6I GCGC 6 cut(s) 46, 57, 122, 124, 221, 785
HinP1I GCGC 6 cut(s) 46, 57, 122, 124, 221, 785
HindIII AAGCTT 1 cut(s) 808
HinfI GANTC 8 cut(s) 66, 104, 235, 344, 437, 463, 478, 607
HpaII CCGG 5 cut(s) 43, 263, 276, 505, 511
HphI GGTGA 1 cut(s) 788
Hpy166II GTNNAC 2 cut(s) 144, 1025
Hpy188I TCNGA 9 cut(s) 103, 109, 240, 310, 357, 442, 477, 483, 889
Hpy188III TCNNGA 3 cut(s) 163, 863, 976
Hpy8I GTNNAC 2 cut(s) 144, 1025
Hpy99I CGWCG 3 cut(s) 205, 357, 523
HpyAV CCTTC 6 cut(s) 47, 169, 453, 562, 679, 979
HpyCH4III ACNGT 1 cut(s) 917
HpyCH4IV ACGT 1 cut(s) 921
HpyCH4V TGCA 5 cut(s) 632, 740, 782, 884, 951
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 54, 268, 677
HpyF3I CTNAG 1 cut(s) 681
HpySE526I ACGT 1 cut(s) 921
Hsp92I GRCGYC 2 cut(s) 57, 352
Hsp92II CATG 3 cut(s) 706, 866, 1014
HspAI GCGC 6 cut(s) 46, 57, 122, 124, 221, 785
KasI GGCGCC 1 cut(s) 56
Kzo9I GATC 3 cut(s) 225, 849, 1018
LguI GCTCTTC 1 cut(s) 542
LmnI GCTCC 7 cut(s) 268, 363, 420, 501, 507, 513, 844
Lsp1109I GCAGC 3 cut(s) 644, 794, 844
LweI GCATC 3 cut(s) 727, 769, 893
MaeI CTAG 4 cut(s) 192, 300, 746, 800
MaeII ACGT 1 cut(s) 921
MaeIII GTNAC 1 cut(s) 917
MalI GATC 3 cut(s) 227, 851, 1020
MbiI CCGCTC 1 cut(s) 847
MboI GATC 3 cut(s) 225, 849, 1018
MboII GAAGA 7 cut(s) 44, 147, 262, 268, 559, 664, 785
MfeI CAATTG 1 cut(s) 1036
MflI RGATCY 1 cut(s) 225
MhlI GDGCHC 1 cut(s) 518
MluCI AATT 5 cut(s) 170, 560, 602, 725, 1036
Mly113I GGCGCC 1 cut(s) 57
MlyI GAGTC 2 cut(s) 353, 472
MmeI TCCRAC 2 cut(s) 335, 688
MspA1I CMGCKG 1 cut(s) 635
MspI CCGG 5 cut(s) 43, 263, 276, 505, 511
MspR9I CCNGG 1 cut(s) 43
MunI CAATTG 1 cut(s) 1036
MvnI CGCG 3 cut(s) 48, 124, 126
MwoI GCNNNNNNNGC 4 cut(s) 17, 54, 268, 677
NarI GGCGCC 1 cut(s) 57
NciI CCSGG 1 cut(s) 43
NdeII GATC 3 cut(s) 225, 849, 1018
NlaIII CATG 3 cut(s) 706, 866, 1014
NlaIV GGNNCC 3 cut(s) 58, 503, 509
NmeAIII GCCGAG 2 cut(s) 60, 552
NmuCI GTSAC 1 cut(s) 917
PagI TCATGA 1 cut(s) 862
PauI GCGCGC 1 cut(s) 122
PceI AGGCCT 2 cut(s) 78, 182
PciSI GCTCTTC 1 cut(s) 542
PcsI WCGNNNNNNNCGW 2 cut(s) 137, 524
PfeI GAWTC 6 cut(s) 66, 104, 235, 437, 478, 607
PleI GAGTC 2 cut(s) 352, 471
PluTI GGCGCC 1 cut(s) 60
PpsI GAGTC 2 cut(s) 352, 471
PshAI GACNNNNGTC 1 cut(s) 206
Psp124BI GAGCTC 1 cut(s) 518
PspN4I GGNNCC 3 cut(s) 58, 503, 509
PstI CTGCAG 2 cut(s) 634, 953
PsuI RGATCY 1 cut(s) 225
PteI GCGCGC 1 cut(s) 122
RsaI GTAC 2 cut(s) 26, 990
RsaNI GTAC 2 cut(s) 25, 989
SacI GAGCTC 1 cut(s) 518
SapI GCTCTTC 1 cut(s) 542
Sau3AI GATC 3 cut(s) 225, 849, 1018
SchI GAGTC 2 cut(s) 353, 472
ScrFI CCNGG 1 cut(s) 43
SduI GDGCHC 1 cut(s) 518
SfaNI GCATC 3 cut(s) 727, 769, 893
SfcI CTRYAG 2 cut(s) 630, 949
SfoI GGCGCC 1 cut(s) 58
SmlI CTYRAG 1 cut(s) 976
SmoI CTYRAG 1 cut(s) 976
Sse9I AATT 5 cut(s) 170, 560, 602, 725, 1036
SseBI AGGCCT 2 cut(s) 78, 182
SspDI GGCGCC 1 cut(s) 56
SspMI CTAG 4 cut(s) 192, 300, 746, 800
SstI GAGCTC 1 cut(s) 518
StuI AGGCCT 2 cut(s) 78, 182
StyD4I CCNGG 1 cut(s) 41
StyI CCWWGG 2 cut(s) 299, 799
TaaI ACNGT 1 cut(s) 917
TaiI ACGT 1 cut(s) 924
TaqI TCGA 4 cut(s) 210, 233, 347, 729
TaqII GACCGA 1 cut(s) 456
TasI AATT 5 cut(s) 170, 560, 602, 725, 1036
TauI GCSGC 7 cut(s) 20, 23, 51, 62, 295, 298, 526
TfiI GAWTC 6 cut(s) 66, 104, 235, 437, 478, 607
TscAI CASTG 1 cut(s) 922
TseFI GTSAC 1 cut(s) 917
TseI GCWGC 3 cut(s) 632, 782, 857
Tsp45I GTSAC 1 cut(s) 917
TspDTI ATGAA 2 cut(s) 671, 999
TspGWI ACGGA 2 cut(s) 218, 459
TspRI CASTG 1 cut(s) 922
XapI RAATTY 2 cut(s) 170, 725
XmaJI CCTAGG 2 cut(s) 299, 799
XmiI GTMKAC 2 cut(s) 143, 1024
XspI CTAG 4 cut(s) 192, 300, 746, 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.