MD14G1188300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011067
Physical Location & Seq
Reverse (-)
28003658 .. 28006342
2685 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1188300.v1.1.491

Sequence Viewer

Length: 1068 bp
ATGGAGGAGGCAAAAGCGGCGGCGTACTATGACGAGCTGACTCGGCGGGGCGAAGGAGCCGCCAGATTCAAACAAGGCCTCGGCTTCTCTTCGGCAAACTCGGATTCCGAAAATGCCTCTGAGCGCGGCTCAGCGCTGCCGTATTCCACCTCGTCTTCCTTCCTCAGTAAGTTTGTTAAGGCGTCCAGCCCGAAGAAGGAGTCGGAGCTTCAGAAGCAAGCACAGATTGAATCCATACAGAACAAGCTCAAGAGGAAGAAGGCCAACGCGGCGGAGGAAAGAGAGAATAGGCAACCGGCTAGGGTTTCGGAGAGAGAGAATAGGCATTCGAGGAAGCGGAGCAGGAGCAGAGAGAGGCATTCGATTCGGAGAAGTAGGAGCAGAGAGAGGTATAGAGAAAGAGATAGGGGTAGAGAGAGGAGGAGGATGAGGTCTGAGAGCGATTCCGATGGAGGTAGACGACGGAGATCGAGAAGCCGGAGTCGGAGTCCGTCGCCTCGGGATCGGAGAAAGGAGCGGAGGAGGAGTCGGAGCTCGTCACCGAGGGAGAGGGGTTCGGAGAGAAGCAAAGGTAAAGCGGGGAAGGAGAGGAATGGAGGCAGTGCTGATCATTCCAAGTTGATTGAAGGCTATGATAAGATGTCAGCGGCCGAGAGAGTGAAGGCGAAGATGAAGCTTCAACTTGCTGAAACTGCTGAGAAGGATACGACGAAAGGGATGGGCTCGGGTTGGGAAAGATTCGAGTTCAACAAAGATGCACCACTTGATGATGAAGACGTTGAAGTTGCTGAAGATGATGCAGCATTAGTTAAGCACATTGGCCAAAGCTTCCGGTTTTCTGCAGTTGAGACAAAAAGGGAGGAGCAGCTCAGATCTGCTCATGATGAGGCTATGTTTGGTGCATCAGATGATCGACCGACCATTGTGATGGATAGTGAGATTGAGGCAGAGAACAACATAAACGACAGCATAGAAAGTGAGCCTACAACTACAAGCCTCCTGAGTGAGAAGGTACTTTCAAAGAAACAAGGTTCATGGCGTGATCGTATACGTGACAAAGGGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

356

Amino Acids

40.62

Weight (kDa)

10.09

Isoelectric Point (pI)

88.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011067)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48120 AT3G48120
fragaria_vesca FvH4_5g16290
malus_domestica MD06G1182300.v1.1 MD14G1188300.v1.1
prunus_persica Prupe.5G180400_v2.0.a1
pyrus_communis pycom06g16120
rosa_chinensis RchiOBHm_Chr7g0179841
rosa_laevigata RLG00000005319
rosa_multiflora Rmu_sc0001754.1_g000002 Rmu_sc0004156.1_g000013 Rmu_sc0035735.1_g000001
rosa_roxburghii Rroxscaffold_3G00273410
rosa_rugosa Rorug06G0428800
rosa_samantha Rh7AG028500 Rh7BG027500 Rh7BG027600 Rh7CG029400 Rh7DG028800
rosa_wichuraiana Rw7G002280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 517
AccI GTMKAC 2 cut(s) 457, 1048
AccII CGCG 2 cut(s) 126, 269
AclWI GGATC 1 cut(s) 510
AcoI YGGCCR 2 cut(s) 648, 820
AcuI CTGAAG 2 cut(s) 194, 810
AcyI GRCGYC 1 cut(s) 182
AfaI GTAC 2 cut(s) 26, 1014
AfeI AGCGCT 1 cut(s) 135
AfiI CCNNNNNNNGG 1 cut(s) 196
AgsI TTSAA 7 cut(s) 70, 230, 626, 680, 748, 782, 1020
AluBI AGCT 7 cut(s) 37, 208, 247, 534, 676, 828, 868
AluI AGCT 7 cut(s) 37, 208, 247, 534, 676, 828, 868
Alw21I GWGCWC 1 cut(s) 536
Alw26I GTCTC 1 cut(s) 842
AlwI GGATC 1 cut(s) 510
Ama87I CYCGRG 2 cut(s) 498, 724
Aor51HI AGCGCT 1 cut(s) 135
AoxI GGCC 4 cut(s) 76, 261, 648, 820
ApeKI GCWGC 3 cut(s) 136, 800, 865
ArsI GACNNNNNNTTYG 2 cut(s) 185, 217
AspLEI GCGC 2 cut(s) 126, 136
AsuHPI GGTGA 1 cut(s) 531
AvaI CYCGRG 2 cut(s) 498, 724
BalI TGGCCA 1 cut(s) 822
BanII GRGCYC 2 cut(s) 536, 725
BbsI GAAGAC 2 cut(s) 147, 780
Bbv12I GWGCWC 1 cut(s) 536
BbvI GCAGC 3 cut(s) 123, 812, 877
BccI CCATC 3 cut(s) 443, 712, 922
BceAI ACGGC 1 cut(s) 124
BcgI CGANNNNNNTGC 2 cut(s) 347, 381
BciVI GTATCC 1 cut(s) 697
BclI TGATCA 1 cut(s) 607
BcoDI GTCTC 1 cut(s) 842
BfaI CTAG 1 cut(s) 300
BfmI CTRYAG 1 cut(s) 840
BfoI RGCGCY 1 cut(s) 137
BfuI GTATCC 1 cut(s) 697
BglI GCCNNNNNGGC 1 cut(s) 269
BglII AGATCT 1 cut(s) 872
BisI GCNGC 9 cut(s) 18, 21, 60, 127, 137, 270, 648, 801, 866
BlpI GCTNAGC 1 cut(s) 130
BlsI GCNGC 9 cut(s) 19, 22, 61, 128, 138, 271, 649, 802, 867
BmeT110I CYCGRG 2 cut(s) 498, 724
BmiI GGNNCC 1 cut(s) 58
BmsI GCATC 3 cut(s) 745, 787, 911
BpiI GAAGAC 2 cut(s) 147, 780
BplI GAGNNNNNCTC 2 cut(s) 113, 145
Bpu1102I GCTNAGC 1 cut(s) 130
BpuEI CTTGAG 1 cut(s) 233
BsaAI YACGTR 1 cut(s) 1052
BsaHI GRCGYC 1 cut(s) 182
BsaJI CCNNGG 3 cut(s) 79, 497, 542
BsaWI WCCGGW 1 cut(s) 831
BsaXI ACNNNNNCTCC 4 cut(s) 472, 502, 511, 541
Bsc4I CCNNNNNNNGG 1 cut(s) 196
Bse118I RCCGGY 1 cut(s) 295
BseDI CCNNGG 3 cut(s) 79, 497, 542
BseGI GGATG 2 cut(s) 432, 723
BseLI CCNNNNNNNGG 1 cut(s) 196
BseMII CTCAG 7 cut(s) 111, 144, 178, 426, 687, 883, 992
BseRI GAGGAG 6 cut(s) 20, 433, 436, 535, 538, 875
BseX3I CGGCCG 1 cut(s) 648
BseXI GCAGC 3 cut(s) 123, 812, 877
Bsh1236I CGCG 2 cut(s) 126, 269
Bsh1285I CGRYCG 2 cut(s) 651, 917
BshFI GGCC 4 cut(s) 78, 263, 650, 822
BsiEI CGRYCG 2 cut(s) 651, 917
BsiHKAI GWGCWC 1 cut(s) 536
BsiHKCI CYCGRG 2 cut(s) 498, 724
BsiSI CCGG 3 cut(s) 296, 478, 832
BslI CCNNNNNNNGG 1 cut(s) 196
BsmAI GTCTC 1 cut(s) 842
BsmI GAATGC 2 cut(s) 325, 358
BsnI GGCC 4 cut(s) 78, 263, 650, 822
BsoBI CYCGRG 2 cut(s) 498, 724
Bsp1286I GDGCHC 2 cut(s) 536, 725
Bsp143I GATC 6 cut(s) 467, 502, 607, 872, 910, 1042
Bsp1720I GCTNAGC 1 cut(s) 130
BspANI GGCC 4 cut(s) 78, 263, 650, 822
BspCNI CTCAG 7 cut(s) 112, 143, 177, 427, 688, 882, 993
BspFNI CGCG 2 cut(s) 126, 269
BspHI TCATGA 1 cut(s) 880
BspLI GGNNCC 1 cut(s) 58
BspMAI CTGCAG 1 cut(s) 844
BspPI GGATC 1 cut(s) 510
BsrBI CCGCTC 1 cut(s) 517
BsrFI RCCGGY 1 cut(s) 295
BssAI RCCGGY 1 cut(s) 295
BssECI CCNNGG 3 cut(s) 79, 497, 542
BssMI GATC 6 cut(s) 467, 502, 607, 872, 910, 1042
BssNAI GTATAC 1 cut(s) 1049
BssNI GRCGYC 1 cut(s) 182
Bst1107I GTATAC 1 cut(s) 1049
Bst6I CTCTTC 1 cut(s) 94
BstACI GRCGYC 1 cut(s) 182
BstBAI YACGTR 1 cut(s) 1052
BstC8I GCNNGC 1 cut(s) 219
BstDEI CTNAG 7 cut(s) 120, 130, 164, 435, 696, 869, 1001
BstF5I GGATG 2 cut(s) 432, 723
BstFNI CGCG 2 cut(s) 126, 269
BstH2I RGCGCY 1 cut(s) 137
BstHHI GCGC 2 cut(s) 126, 136
BstKTI GATC 6 cut(s) 470, 505, 610, 875, 913, 1045
BstMAI GTCTC 1 cut(s) 842
BstMBI GATC 6 cut(s) 467, 502, 607, 872, 910, 1042
BstMCI CGRYCG 2 cut(s) 651, 917
BstMWI GCNNNNNNNGC 5 cut(s) 17, 43, 214, 269, 692
BstSFI CTRYAG 1 cut(s) 840
BstUI CGCG 2 cut(s) 126, 269
BstV1I GCAGC 3 cut(s) 123, 812, 877
BstV2I GAAGAC 2 cut(s) 147, 780
BstX2I RGATCY 1 cut(s) 872
BstXI CCANNNNNNTGG 1 cut(s) 928
BstYI RGATCY 1 cut(s) 872
BstZ17I GTATAC 1 cut(s) 1049
BstZI CGGCCG 1 cut(s) 648
BsuI GTATCC 1 cut(s) 697
BsuRI GGCC 4 cut(s) 78, 263, 650, 822
BtsCI GGATG 2 cut(s) 432, 723
BtsI GCAGTG 1 cut(s) 607
BtsIMutI CAGTG 1 cut(s) 607
Cac8I GCNNGC 1 cut(s) 219
CciI TCATGA 1 cut(s) 880
CfoI GCGC 2 cut(s) 126, 136
Cfr10I RCCGGY 1 cut(s) 295
CseI GACGC 1 cut(s) 171
Csp6I GTAC 2 cut(s) 25, 1013
CviAII CATG 2 cut(s) 881, 1035
CviQI GTAC 2 cut(s) 25, 1013
DdeI CTNAG 7 cut(s) 120, 130, 164, 435, 696, 869, 1001
DpnI GATC 6 cut(s) 469, 504, 609, 874, 912, 1044
DpnII GATC 6 cut(s) 467, 502, 607, 872, 910, 1042
EaeI YGGCCR 2 cut(s) 648, 820
EagI CGGCCG 1 cut(s) 648
Eam1104I CTCTTC 1 cut(s) 94
EarI CTCTTC 1 cut(s) 94
EciI GGCGGA 1 cut(s) 287
Ecl136II GAGCTC 1 cut(s) 534
EclXI CGGCCG 1 cut(s) 648
Eco147I AGGCCT 1 cut(s) 78
Eco24I GRGCYC 2 cut(s) 536, 725
Eco47III AGCGCT 1 cut(s) 135
Eco52I CGGCCG 1 cut(s) 648
Eco53kI GAGCTC 1 cut(s) 534
Eco57I CTGAAG 2 cut(s) 194, 810
Eco88I CYCGRG 2 cut(s) 498, 724
EcoICRI GAGCTC 1 cut(s) 534
EcoT38I GRGCYC 2 cut(s) 536, 725
FaeI CATG 2 cut(s) 884, 1038
FatI CATG 2 cut(s) 880, 1034
FauI CCCGC 2 cut(s) 39, 571
FbaI TGATCA 1 cut(s) 607
FblI GTMKAC 2 cut(s) 457, 1048
Fnu4HI GCNGC 9 cut(s) 18, 21, 60, 127, 137, 270, 648, 801, 866
FokI GGATG 2 cut(s) 439, 730
FriOI GRGCYC 2 cut(s) 536, 725
Fsp4HI GCNGC 9 cut(s) 18, 21, 60, 127, 137, 270, 648, 801, 866
FspBI CTAG 1 cut(s) 300
GlaI GCGC 2 cut(s) 125, 135
GluI GCNGC 9 cut(s) 18, 21, 60, 127, 137, 270, 648, 801, 866
HaeII RGCGCY 1 cut(s) 137
HaeIII GGCC 4 cut(s) 78, 263, 650, 822
HapII CCGG 3 cut(s) 296, 478, 832
HgaI GACGC 1 cut(s) 171
HhaI GCGC 2 cut(s) 126, 136
Hin1I GRCGYC 1 cut(s) 182
Hin1II CATG 2 cut(s) 884, 1038
Hin6I GCGC 2 cut(s) 124, 134
HinP1I GCGC 2 cut(s) 124, 134
HindIII AAGCTT 2 cut(s) 674, 826
HpaII CCGG 3 cut(s) 296, 478, 832
HphI GGTGA 1 cut(s) 531
Hpy166II GTNNAC 2 cut(s) 458, 1049
Hpy188III TCNNGA 5 cut(s) 250, 471, 500, 881, 1000
Hpy8I GTNNAC 2 cut(s) 458, 1049
Hpy99I CGWCG 3 cut(s) 465, 496, 712
HpyAV CCTTC 9 cut(s) 47, 169, 190, 253, 577, 620, 655, 694, 1003
HpyCH4IV ACGT 2 cut(s) 777, 1051
HpyCH4V TGCA 4 cut(s) 758, 800, 842, 902
HpyF10VI GCNNNNNNNGC 5 cut(s) 17, 43, 214, 269, 692
HpyF3I CTNAG 7 cut(s) 120, 130, 164, 435, 696, 869, 1001
HpySE526I ACGT 2 cut(s) 777, 1051
Hsp92I GRCGYC 1 cut(s) 182
Hsp92II CATG 2 cut(s) 884, 1038
HspAI GCGC 2 cut(s) 124, 134
Ksp22I TGATCA 1 cut(s) 607
Kzo9I GATC 6 cut(s) 467, 502, 607, 872, 910, 1042
LmnI GCTCC 8 cut(s) 56, 205, 339, 345, 378, 514, 531, 862
LpnPI CCDG 7 cut(s) 76, 199, 309, 328, 491, 845, 1013
Lsp1109I GCAGC 3 cut(s) 123, 812, 877
LweI GCATC 3 cut(s) 745, 787, 911
MaeI CTAG 1 cut(s) 300
MaeII ACGT 2 cut(s) 777, 1051
MaeIII GTNAC 2 cut(s) 537, 1052
MalI GATC 6 cut(s) 469, 504, 609, 874, 912, 1044
MbiI CCGCTC 1 cut(s) 517
MboI GATC 6 cut(s) 467, 502, 607, 872, 910, 1042
MboII GAAGA 7 cut(s) 81, 147, 205, 268, 679, 785, 803
MflI RGATCY 1 cut(s) 872
MhlI GDGCHC 2 cut(s) 536, 725
MlsI TGGCCA 1 cut(s) 822
MluCI AATT 1 cut(s) 1063
MluNI TGGCCA 1 cut(s) 822
MlyI GAGTC 5 cut(s) 34, 209, 490, 496, 535
MmeI TCCRAC 3 cut(s) 183, 464, 509
Mox20I TGGCCA 1 cut(s) 822
MscI TGGCCA 1 cut(s) 822
MseI TTAA 2 cut(s) 177, 810
MslI CAYNNNNRTG 1 cut(s) 926
Msp20I TGGCCA 1 cut(s) 822
MspA1I CMGCKG 1 cut(s) 647
MspI CCGG 3 cut(s) 296, 478, 832
Mva1269I GAATGC 2 cut(s) 325, 358
MvnI CGCG 2 cut(s) 126, 269
MwoI GCNNNNNNNGC 5 cut(s) 17, 43, 214, 269, 692
NdeII GATC 6 cut(s) 467, 502, 607, 872, 910, 1042
NlaIII CATG 2 cut(s) 884, 1038
NlaIV GGNNCC 1 cut(s) 58
NmeAIII GCCGAG 3 cut(s) 22, 60, 676
NmuCI GTSAC 2 cut(s) 537, 1052
PagI TCATGA 1 cut(s) 880
PceI AGGCCT 1 cut(s) 78
PctI GAATGC 2 cut(s) 325, 358
PfeI GAWTC 6 cut(s) 66, 104, 230, 364, 443, 738
PkrI GCNGC 9 cut(s) 19, 22, 61, 128, 138, 271, 649, 802, 867
PleI GAGTC 5 cut(s) 34, 208, 489, 495, 534
PpsI GAGTC 5 cut(s) 34, 208, 489, 495, 534
Ppu21I YACGTR 1 cut(s) 1052
Psp124BI GAGCTC 1 cut(s) 536
PspN4I GGNNCC 1 cut(s) 58
PstI CTGCAG 1 cut(s) 844
PsuI RGATCY 1 cut(s) 872
RsaI GTAC 2 cut(s) 26, 1014
RsaNI GTAC 2 cut(s) 25, 1013
RseI CAYNNNNRTG 1 cut(s) 926
SacI GAGCTC 1 cut(s) 536
SaqAI TTAA 2 cut(s) 177, 810
SatI GCNGC 9 cut(s) 18, 21, 60, 127, 137, 270, 648, 801, 866
Sau3AI GATC 6 cut(s) 467, 502, 607, 872, 910, 1042
SchI GAGTC 5 cut(s) 34, 209, 490, 496, 535
SduI GDGCHC 2 cut(s) 536, 725
SfaNI GCATC 3 cut(s) 745, 787, 911
SfcI CTRYAG 1 cut(s) 840
SmiMI CAYNNNNRTG 1 cut(s) 926
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 1 cut(s) 1063
SseBI AGGCCT 1 cut(s) 78
SspMI CTAG 1 cut(s) 300
SstI GAGCTC 1 cut(s) 536
StuI AGGCCT 1 cut(s) 78
TaiI ACGT 2 cut(s) 780, 1054
TaqI TCGA 5 cut(s) 329, 362, 470, 741, 913
TaqII GACCGA 1 cut(s) 931
TasI AATT 1 cut(s) 1063
TauI GCSGC 6 cut(s) 20, 23, 62, 129, 272, 650
TfiI GAWTC 6 cut(s) 66, 104, 230, 364, 443, 738
Tru1I TTAA 2 cut(s) 177, 810
Tru9I TTAA 2 cut(s) 177, 810
TscAI CASTG 1 cut(s) 607
TseFI GTSAC 2 cut(s) 537, 1052
TseI GCWGC 3 cut(s) 136, 800, 865
Tsp45I GTSAC 2 cut(s) 537, 1052
TspDTI ATGAA 3 cut(s) 686, 786, 1023
TspGWI ACGGA 2 cut(s) 478, 480
TspRI CASTG 1 cut(s) 607
XmiI GTMKAC 2 cut(s) 457, 1048
XspI CTAG 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.